JP5444437B2 - Method and composition for liquid mixture of non-detoxified sulfur and useful microorganisms for prevention and control and crop growth - Google Patents

Method and composition for liquid mixture of non-detoxified sulfur and useful microorganisms for prevention and control and crop growth Download PDF

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JP5444437B2
JP5444437B2 JP2012224586A JP2012224586A JP5444437B2 JP 5444437 B2 JP5444437 B2 JP 5444437B2 JP 2012224586 A JP2012224586 A JP 2012224586A JP 2012224586 A JP2012224586 A JP 2012224586A JP 5444437 B2 JP5444437 B2 JP 5444437B2
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パク、ギョンヒ
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株式会社 ファンセムバイオ
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Description

本発明は、防除防疫及び作物の生育のため、除毒されない硫黄と有用微生物群とを液状混合する方法及びその組成物に関するものである。詳細には、水に溶解されず、除毒されない硫黄を有用微生物群と液状混合して作物の薬剤防除のための効果はもちろん、作物の細胞膜を丈夫にし、かつ栄養分の細胞内の吸収を円滑に助けて作物がよく育つようにするだけでなく、硫黄の強抗酸化作用により作物の保存性を高めて、増収効果及び品質が向上し、硫黄の殺菌、
殺虫力により土壌に含まれている有害な微生物を死滅及び増殖を抑制することにより、有用微生物を活性化させて有用微生物の力価を高めることができる防除防疫及び作物生育のために、除毒されない硫黄と有用微生物群とを液状混合する方法及びその組成物に関する。
The present invention relates to a method of liquid mixing sulfur and non-detoxified microorganisms and useful microorganisms for control and prevention and crop growth, and a composition thereof. Specifically, sulfur, which is not dissolved in water and not detoxified, is mixed with useful microorganisms in a liquid state to ensure the effect of crop chemical control, as well as making the cell membrane of the crop strong and smooth absorption of nutrients in the cells. In addition to helping crops grow well, sulfur's strong antioxidant action increases crop preservation and improves yield and quality, sulfur sterilization,
Detoxification for the prevention and control of epidemics and crop growth that can increase the potency of useful microorganisms by activating useful microorganisms by killing and suppressing the harmful microorganisms contained in the soil by insecticidal power The present invention relates to a method of mixing liquid sulfur and useful microorganisms and a composition thereof.

硫黄は、土壌の7大必須栄養素であり地球上のすべての有機体の必須元素であるとともに、硫黄特有の毒性を持っていて害虫と有害菌防除にも効果が卓越すると知られている。近年、生活の質が高くなり消費者が有機農産物を多く求めるようになると、化学肥料や農薬の代わりに硫黄を害虫と有害菌防除及び作物生育に使用して親環境農産物を生産しようとする農家が増えている。   Sulfur is one of the seven essential nutrients in the soil and is an essential element of all living organisms on the earth. It is also known to have a peculiar effect on the control of pests and harmful fungi due to its toxicity. In recent years, when the quality of life has improved and consumers are demanding a lot of organic agricultural products, farmers are trying to produce environmentally friendly agricultural products by using sulfur instead of chemical fertilizers and pesticides for pest and harmful bacteria control and crop growth. Is increasing.

農薬の代わりに硫黄で効果的な薬剤防除をするためには、硫黄をすべての植物に効果的に塗布するための液状化が先行されなければならないが、硫黄は水に溶解されないため、粉末状で植物と土壌に直接撤かざるを得なくなる。
このような場合、硫黄粉末が風により飛ばされたり凝集されたりするなど、作物に均等に粘着されないため薬剤防除効果が低下し、特に、粉末状であるため、植物の吸収率が低くなり必要な時期に植物の栄養素的機能も期待することができない。
また、粉末硫黄は、作業者が不注意に特定地域、特定部位に適正量を超過して過量散布する場合、その周辺の作物と土壌微生物に致命的なことがあり、粉末状の硫黄は、広域散布が可能な液状に比べて、作業能率が劣るため、結局、生産費用の上昇の要因となる。
In order to effectively control chemicals with sulfur instead of pesticides, liquefaction must be preceded to effectively apply sulfur to all plants. It must be removed directly to the plant and soil.
In such a case, the sulfur powder is not evenly adhered to the crop, such as being blown or agglomerated by the wind, so the chemical control effect is reduced. It is not possible to expect the nutrient function of plants at any time.
In addition, powdered sulfur may be fatal to the surrounding crops and soil microorganisms if the worker inadvertently overspreads the appropriate amount over a specific area or site, and the powdered sulfur may be fatal. Since the work efficiency is inferior compared to liquids that can be spread over a wide area, it eventually causes an increase in production costs.

このように硫黄は、すべての植物の栄養素的機能と害虫、有害菌を退治死滅する薬剤防除の機能も持っているが、粉末状の硫黄は、液状に比べて植物の円滑な吸収を期待し難く、植物の葉や幹に適正水準まで癒着されないため、持続的な栄養供給と薬剤防除を遂行し難い問題点があった。   In this way, sulfur has the nutrient function of all plants, and also has the function of controlling chemicals to combat and kill pests and harmful bacteria, but powdered sulfur is expected to absorb plants more smoothly than liquid. It is difficult, and since it does not adhere to plant leaves and stems to an appropriate level, there is a problem that it is difficult to carry out continuous nutrient supply and chemical control.

従来には、水に溶解されない硫黄を液状化することを目的に、石灰と硫黄を混合、高熱で沸かして粘液質の状態(石灰硫黄合剤)に作って、20〜40ccを20リットルの水に溶いて使用したり、 硫黄を苛性ソーダ(NaOH)に溶かして液状化した後、黄土とその他の農業資材を混合した黄土硫黄合剤を作って使用している。
また、近年には、硫黄粉末を液状化するため、洗濯石鹸液、台所用洗剤などを硫黄粉末とともにミキサーで高速回転して水に溶けるようにするコロイド化する方法を用いたこともある。
また、界面活性剤である糖蜜と硫黄粉末をミキサーで高速回転して粘液質化した後、水に溶いて使用しているが、この方法は糖蜜の糖分により、むしろ有害菌と害虫が群集する問題が現れて、一部の耕作者は、有害菌、害虫の群集を予防するため、糖蜜に過酸化水素、塩素などを添加していることが実情である。
Conventionally, for the purpose of liquefying sulfur that is not dissolved in water, lime and sulfur are mixed and boiled with high heat to make a mucous state (lime sulfur mixture), and 20 to 40 cc is made into 20 liters of water. It is used after being dissolved, or sulfur is dissolved in caustic soda (NaOH) and liquefied, and then a loess sulfur mixture is prepared by mixing ocher and other agricultural materials.
In recent years, in order to liquefy sulfur powder, a colloidalization method has been used in which laundry soap solution, kitchen detergent and the like are dissolved together with sulfur powder by high-speed rotation with a mixer.
In addition, the molasses and sulfur powder, which are surfactants, are turned into a mucus by rotating at high speed with a mixer and then used by dissolving in water. However, this method has a problem that harmful fungi and pests gather due to the sugar content of molasses. In fact, some farmers are adding hydrogen peroxide, chlorine, etc. to molasses to prevent harmful fungi and pest communities.

しかし、従来の硫黄を加熱して液状化する方法は、硫黄を加熱する過程で悪臭及び有毒ガスが発生、作業者の健康と安全を脅かすなど過程が非常に煩わしく、硫黄を苛性ソーダ(NaOH−pH13)、即ち、灰汁に溶かして液状化する黄土硫黄合剤方法は、作業者はもちろん、作物にも非常に危ないだけでなく、消費者も灰汁を用いて生産する農産物を忌避するため、好ましくない。
また、硫黄粉末を洗濯石鹸液や台所用洗剤を界面活性剤として用いて液状化する方法は、土壌と河川に富栄養化現象をもたらすなど、他の汚染源となる問題点があり、消費者も洗濯石鹸や台所用洗剤が付いた農産物を忌避するようになり問題がある。
また、 糖蜜の糖分により有害菌と害虫が群集する問題点を解決するため、糖蜜に過酸化水素、塩素などを添加しなければならないが、これは植物の細胞を損傷させる致命的な物質であるため、この方法も好ましくないという結論に到逹した。
However, the conventional method of liquefaction by heating sulfur is very troublesome in the process of heating sulfur, generating bad odors and toxic gases, threatening the health and safety of workers. ) That is, the loess sulfur mixture method that is dissolved in lye and liquefied is not preferable because it is not only dangerous for workers but also for crops, and consumers also avoid agricultural products produced using lye. .
In addition, the method of liquefying sulfur powder using laundry soap solution or kitchen detergent as a surfactant has other problems that cause eutrophication to soils and rivers, and causes other sources of pollution. There is a problem because it avoids agricultural products with laundry soap and kitchen detergent.
In addition, in order to solve the problem of harmful bacteria and pests crowding due to the sugar content of molasses, it is necessary to add hydrogen peroxide, chlorine, etc. to molasses, which is a deadly substance that damages the cells of plants. Therefore, we came to the conclusion that this method is also not preferable.

そこで、本発明は、上記従来技術の問題点を解決し、防除防疫及び作物生育のために硫黄を好適に適用できる方法及びその組成物を提供することを課題とする。   Then, this invention makes it a subject to solve the said problem of the said prior art, and to provide the method and composition which can apply sulfur suitably for control prevention and epidemic and crop growth.

本発明の防除防疫及び作物の生育用に用いるため、除毒されない硫黄と有用微生物群を液状混合する方法は、EM原液、活性液、醗酵液を含む液状形態の有用微生物群(EM:Effective Microorganism)と除毒されない硫黄粉末とを1:1ないし99:1の体積割合で混合した液状混合物を保存する保存槽の内外部に加熱装置を設けて間接加熱で25〜60℃の温度が保持された状態で24時間放置するステップと、その後、除毒されない硫黄と有用微生物群の液状混合物を木棒や撹拌装置で撹拌するステップと、からなることを特徴とする。   The method for liquid mixing of non-detoxified sulfur and useful microorganisms for use in the control and prevention of the present invention and crop growth is based on a liquid form of useful microorganisms (EM: Effective Microorganism including EM stock solution, active solution and fermentation broth). ) And non-detoxified sulfur powder mixed at a volume ratio of 1: 1 to 99: 1, a heating device is provided inside and outside the storage tank to maintain a temperature of 25 to 60 ° C. by indirect heating. And a step of stirring the liquid mixture of non-detoxified sulfur and useful microorganisms with a wooden stick or a stirring device.

また、本発明の防除防疫及び作物の生育用に用いるため、除毒されない硫黄と有用微生物群を液状混合した組成物は、EM原液、 活性液、 醗酵液を含む液状形態の有用微生物群(EM:Effective Microorganism)と除毒されない硫黄粉末を1:1 ないし99:1の体積割合で混合した液状混合物を保存する保存槽の内外部に加熱装置を設けて間接加熱で25〜60℃の温度が保持された状態で24時間放置するステップと、その後、除毒されない硫黄と有用微生物群の液状混合物を木棒や撹拌装置で撹拌するステップと、により製造されたものであることを特徴とする。   In addition, the composition of liquid mixture of non-detoxified sulfur and useful microorganism group for use in the control and prevention of the present invention and crop growth is a liquid form of useful microorganism group (EM : Effective Microorganism) and sulfur powder that is not detoxified mixed in a volume ratio of 1: 1 to 99: 1. A heating device is provided inside and outside the storage tank for storing the liquid mixture. It is produced by a step of leaving it for 24 hours in a held state and a step of stirring a liquid mixture of non-detoxified sulfur and useful microorganisms with a wooden stick or a stirring device.

本発明によると、水に溶解されない、除毒されない硫黄を有用微生物群と液状混合して作物の薬剤防除のための効果はもちろん、作物の細胞膜を丈夫にし、栄養分の細胞内の吸収を円滑に助けて作物をよく育つようにするだけでなく、強抗酸化作用により作物の保存性を高めて、増収効果及び品質が向上される効果がある。
また、硫黄の殺菌、殺虫力により土壌に含まれている有害な微生物を死滅させたり、増殖を抑制することにより、土壌を消毒し、土壌内の有用微生物を活性化させて有用微生物の力価を高めることができる有用な発明である。
According to the present invention, sulfur, which is not dissolved in water and not detoxified, is mixed with useful microorganisms in a liquid state, as well as an effect for controlling the chemicals of the crop, making the cell membrane of the crop strong and smooth absorption of nutrients in the cells. In addition to helping the crops grow well, it has the effect of increasing the yield and quality of the crops by increasing the storage stability of the crops through strong antioxidant action.
In addition, by killing harmful microorganisms contained in the soil by sterilizing sulfur and killing insects, or suppressing the growth, the soil is disinfected and the useful microorganisms in the soil are activated. It is a useful invention that can enhance the

先ず、除毒されない硫黄を粉末状に作った後、除毒されない硫黄をえさにして活動する有用微生物群の菌を混合する。
前記のように有用微生物群と除毒されない硫黄粉末を混合する時の割合は、1:1ないし99: 1の体積割合で混合する。
ここで、前記有用微生物群(EM:Effective Microorganism)とは、乳酸菌群、 酵母菌群、 光合性菌群を含む。
First, sulfur that is not detoxified is made into a powder form, and then a group of useful microorganisms that are active on the sulfur that is not detoxified is mixed.
As described above, the mixing ratio of the useful microorganism group and the non-detoxified sulfur powder is 1: 1 to 99: 1.
Here, the useful microorganism group (EM: Effective Microorganism) includes a lactic acid bacteria group, a yeast group, and a photosynthesis bacteria group.

一方、前記の割合で液状混合した状態で25〜60℃の温度が保持されるようにしながら24時間を放置すると、
硫黄をえさにして活動する有用微生物群の活性液が触れる部分から除毒されない硫黄が徐々に沈み始める。
その後、2日目からは除毒されない硫黄と有用微生物群の液状混合物を木棒や攪拌機装置を用いてかき混ぜるが、これは、有用微生物群の菌の中で好気性微生物の活性化のため、適正量の空気が入るようにして活性を誘導するとともに、除毒されない硫黄と有用微生物群を撹拌して早く混合されるようにするためのものである。
On the other hand, if 24 hours is allowed to stand while maintaining a temperature of 25 to 60 ° C. in the liquid mixture at the above ratio,
Sulfur, which is not detoxified, gradually begins to sink from the area where the active liquid of useful microorganisms that are active on sulfur is in contact.
Then, from the second day, the liquid mixture of sulfur and useful microorganisms that are not detoxified is stirred using a wooden stick or a stirrer device. This is because of the activation of aerobic microorganisms among the bacteria of the useful microorganisms. The activity is induced by allowing an appropriate amount of air to enter, and the non-detoxified sulfur and the useful microorganism group are agitated to be mixed quickly.

有用微生物群の菌の中で植物の光合性活動に有用であり、植物を丈夫で健康にする微生物である光合性菌は、25〜60℃で活性度が最も高く、温度が下がるほど活性が鈍化され、5℃以下で生命活動がほとんど停止するため、常に25〜60℃を保持することが好ましい。
もちろん、乳酸菌、酵母のような一部微生物は、60℃を超過しても活性度が高いものの、植物の生長、発育のために光合性菌が必ず必要であるため、有用微生物群の活性液の最高温度は、60℃を超過してはならないのである。
Among the microorganisms of the useful microorganism group, the photosynthesis bacteria that are useful for the photosynthesis activity of the plant and that make the plant strong and healthy have the highest activity at 25 to 60 ° C., and the activity decreases as the temperature decreases. It is preferable that the temperature is always maintained at 25 to 60 ° C. because the life activity is almost stopped at 5 ° C. or less.
Of course, although some microorganisms such as lactic acid bacteria and yeast have high activity even when the temperature exceeds 60 ° C., photosynthesis bacteria are indispensable for the growth and development of plants. The maximum temperature should not exceed 60 ° C.

このように有用微生物群の活性液の適正温度を保持するため、保存槽の加熱装置に温度調節装置を付けると、作業者が現場を離れていても望みの温度を続けて保持することができるため、便利である。
この時、加熱装置を有用微生物群の活性液に直接投入、加熱することになると、加熱器に近接した微生物が高温により死滅する恐れがあるため、保存槽の内外部に加熱装置を設けて間接加熱することが好ましい。
In order to maintain the appropriate temperature of the active liquid of the useful microorganism group in this way, if a temperature control device is attached to the heating device of the storage tank, the desired temperature can be continuously maintained even if the worker is away from the site. Because it is convenient.
At this time, if the heating device is directly put into the active liquid of the useful microorganism group and heated, the microorganisms close to the heater may die due to high temperature. It is preferable to heat.

ここで、前記有用微生物群のうち、乳酸菌の種類は、PH4に近い酸性である時、活動性が最も強く作用することになるが、このような有用な乳酸菌の種類は、土壌と作物に使用する時、有害な微生物の生育を抑制したり、またはえさ活動を阻害するため、有害微生物を死滅させる役目をすることになる。
このような有用微生物群の活性液に含まれる除毒されない硫黄は、特有の殺菌、殺虫力により土壌と作物の有害微生物の死滅及び増殖を抑制しながら、 有用微生物群を活性化させ、除毒されない硫黄は、有用微生物群中の一部の乳酸菌の代謝活動に用いられるが、このような過程で生成される代謝産物は、土壌内の難分解性物質を可溶化し、これを作物が栄養分として用いることになる。
Here, in the group of useful microorganisms, when the type of lactic acid bacteria is acidic close to PH4, the activity is the strongest, but this kind of useful lactic acid bacteria is used for soil and crops. When doing so, it will serve to kill harmful microorganisms in order to suppress the growth of harmful microorganisms or inhibit food activity.
The non-detoxified sulfur contained in the active liquid of such useful microorganisms activates the useful microorganisms and suppresses detoxification while suppressing the killing and growth of harmful microorganisms in soil and crops by its specific sterilization and insecticidal power. Unused sulfur is used for the metabolic activity of some lactic acid bacteria in the useful microbial community, but the metabolites produced in this process solubilize persistent substances in the soil, which are used by the crop as nutrients. Will be used as

前記のような方法により製造された本発明の防除防疫及び作物の生育のため、除毒されない硫黄と有用微生物群を液状混合する方法により組成された組成物は、一般の水によく希釈されて作物に直接散布することができ、 本願の除毒されない硫黄と有用微生物群の液状混合物が散布された作物は、害虫の中でアブラムシの場合、3日後 76.0%、7日後82.9%の防除効果を表した。
また、有害菌の中で白粉病の場合、作物に散布7日後、85.9%の防除効果が表れることが観察され、栄養供給が同時に可能であるため、作物の細胞膜を丈夫にし、細胞内の栄養分の吸収を円滑に助けて作物をよく育つようにするだけでなく、強抗酸化作用により生産作物の保存性を高め、増収効果及び品質が向上する効果を表した。
For the prevention and control of the present invention and the growth of crops produced by the method as described above, the composition prepared by the liquid mixing of the non-detoxified sulfur and the useful microorganism group is well diluted in general water. In the case of aphids among the pests, 76.0% after 7 days and 82.9% after 7 days when the liquid mixture of non-detoxified sulfur and useful microorganisms that can be applied directly to the crop is sprayed. The control effect of was expressed.
In addition, in the case of white powder disease among harmful bacteria, it was observed that a control effect of 85.9% appeared 7 days after spraying on the crop, and because nutrient supply is possible at the same time, the cell membrane of the crop is made strong, In addition to helping to absorb the nutrients smoothly, the crops grow well, and the effect of increasing the yield and quality is enhanced by enhancing the storage stability of the crops produced by strong antioxidant action.

以下に、実施例を示す。   Examples are shown below.

<実施例1>
唐辛子のワタアブラムシ防除試験:
この試験は、韓国公州大学校産業科学大学に依頼して表れた資料に基づいて作成した。
1.試験目的:優れた製剤を選抜して作物害虫管理用資材に活用しようとする。
2.試験方法:
イ.試験作物(品種):唐辛子(緑光)
ロ.対象害虫:唐辛子ワタアブラムシ(Aphis gossypii)
ハ.対象害虫発生状況:薬剤処理前の無処理区の発生密度が、区当たり平均82.5匹であり、防除効果を検討するに十分である。
ニ.耕種概要: 一般農家慣行栽培法に準じた。 他の害虫の防除のための薬剤散布はなかった。
ホ.試験区配置及び面積:乱塊法3回繰り返し
<Example 1>
Pepper Cotton Aphid Control Test:
This examination was made based on the materials presented at the request of Gongju University of Industrial Science.
1. Test Objective: To select excellent formulations and use them for crop pest management materials.
2. Test method:
Test crop (variety): chili pepper (green light)
B. Target pests: pepper cotton aphid (Aphis gossypii)
C. Status of occurrence of target pests: The average density of the untreated group before chemical treatment is 82.5 per group, which is sufficient for examining the control effect.
D. Cultivation outline: According to the general farming method. There was no drug spray for controlling other pests.
E. Test plot layout and area: repeated 3 times

Figure 0005444437
Figure 0005444437

ヘ.処理内容   Processing contents

Figure 0005444437
Figure 0005444437

ト.薬剤散布前後の気象状況
薬効に影響を及ぼすに値する特別な気象状況はなかった。
G. Weather conditions before and after drug application There were no special weather conditions worthy of affecting drug efficacy.

3.調査方法:   3. Survey method:

Figure 0005444437
Figure 0005444437

4.試験成績:
イ.薬効試験
− 唐辛子ワタアブラムシに対する薬剤防除効果(薬剤処理3日後)
4). Test results:
B. Drug efficacy test-Drug control effect on chili cotton aphid (3 days after drug treatment)

Figure 0005444437
Figure 0005444437

−唐辛子ワタアブラムシに対する薬剤防除効果(薬剤処理7日後)   -Drug control effect on chili pepper aphid (7 days after drug treatment)

Figure 0005444437
Figure 0005444437

ロ.薬害試験
−薬剤処理後3、5、7日後の調査
B) Chemical damage test-Investigation after 3, 5 and 7 days after drug treatment

Figure 0005444437
Figure 0005444437

5.結果要約:
イ.薬効
唐辛子ワタアブラムシに対する試験薬剤である除毒されない硫黄(硫黄+有用微生物)は、薬剤処理3日後、76.0%、薬剤処理7日後、82.9%の防除効果を表した。
ロ.薬害
前記試験薬剤の基準量及び倍量をそれぞれ茎葉処理した後、3回にかけて薬害有無を逹観調査した結果、薬剤処理で症状が観察されなかった。
6.試験担当者意見:
試験薬剤が唐辛子ワタアブラムシに対する防除薬剤として実用性があると判断される。
5. Result summary:
A. Medicinal effect Non-detoxified sulfur (sulfur + useful microorganism), which is a test drug against chili pepper aphid, exhibited a control effect of 76.0% after 3 days of chemical treatment and 82.9% after 7 days of chemical treatment.
(B) Drug damage After the stems and leaves were treated with the reference amount and the doubled amount of the test drug, the symptom was not observed in the drug treatment as a result of a thorough survey on the presence or absence of the drug damage.
6). Examiner opinion:
It is judged that the test drug is practical as a control agent for chili cotton aphid.

<実施例2>
いちご白粉病防除試験:
この試験は、韓国公州大学校産業科学大学に依頼して表れた資料に基づいて作成した。
1.試験目的:優れた製剤を選抜して作物病害管理用資材に活用しようとする。
2.試験方法:
イ.試験作物(品種):いちご(梅香)
ロ.対象病害:いちご白粉病(Sphaerotheca fuliginea)
ハ.対象病害発生状況:薬剤処理前の無処理区のいちご白粉病の発病度は、区当り46.5であり、薬剤間の防除効果を検討するに十分である。
ニ.耕種概要: 一般農家の慣行栽培法に準じた。 他の病害の防除のための薬剤散布はなかった。
ホ.試験区配置及び面積:乱塊壊法3回繰り返し
<Example 2>
Strawberry white powder disease control test:
This examination was made based on the materials presented at the request of Gongju University of Industrial Science.
1. Test purpose: Select excellent products and use them for crop disease management materials.
2. Test method:
A. Test crop (variety): Strawberry (Umeka)
B. Target disease: Strawberry white powder disease (Sphaerotheca fuliginea)
C. Target disease occurrence status: The incidence of strawberry white powder disease in the untreated group before drug treatment is 46.5 per group, which is sufficient for examining the control effect between drugs.
D. Cultivation outline: According to the conventional cultivation method of general farmers. There were no drug sprays to control other diseases.
E. Test plot layout and area: repeated 3 times

Figure 0005444437
Figure 0005444437

ヘ.処理内容   Processing contents

Figure 0005444437
Figure 0005444437

ト.薬剤散布前後の気象状況
薬効に影響を及ぼすに値する特別な気象状況はなかった。
3.調査方法:
G. Weather conditions before and after drug application There were no special weather conditions worthy of affecting drug efficacy.
3. Survey method:

Figure 0005444437
Figure 0005444437

4.試験成績:
イ.薬効試験
−いちご白粉病に対する薬剤防除効果(薬剤処理7日後)
4). Test results:
A. Drug efficacy test-Drug control effect against strawberry white powder disease (7 days after drug treatment)

Figure 0005444437
Figure 0005444437

ロ.薬害試験
−薬剤処理後3、5、7日後の調査
B) Chemical damage test-Investigation after 3, 5 and 7 days after drug treatment

Figure 0005444437
Figure 0005444437

5.結果要約:
イ.薬効
いちご白粉病に対する試験薬剤である除毒されない硫黄(硫黄+有用微生物)は、薬剤処理7日後、85.9%の防除効果を表した。
ロ.薬害
前記試験薬剤の基準量及び倍量をそれぞれ茎葉処理した後、3回にかけて薬害有無を逹観調査した結果、薬剤処理で薬害症状が観察されなかった。
6.試験担当者意見:
試験薬剤が比較的高い防除効果を表して、いちご白粉病に対する防除薬剤に実用性があると判断される。
前記のように本願の除毒されない硫黄と有用微生物群(EM)の液状混合物が散布された唐辛子は、アブラムシの場合、3日後76.0%、7日後82.9%の防除効果が表れ、いちご白粉病の場合、7日後85.9%の防除効果が表れるものと観察された。
5. Result summary:
A. Medicinal effect Non-detoxified sulfur (sulfur + useful microorganisms), which is a test drug for strawberry white powder disease, showed a control effect of 85.9% after 7 days of drug treatment.
B. Chemical damage After the stems and leaves were treated with the reference amount and the doubled amount of the test drug, the presence or absence of the chemical damage was surveyed three times. As a result, no chemical damage symptoms were observed in the drug treatment.
6). Examiner opinion:
Since the test drug exhibits a relatively high control effect, it is judged that the control drug against strawberry white powder disease is practical.
As described above, chili pepper sprayed with a liquid mixture of non-detoxified sulfur and useful microorganisms (EM) of the present application is 76.0% after 3 days and 82.9% after 7 days in the case of aphids. In this case, after 8 days, 85.9% of the control effect was observed.

<実施例3>
2011.4.〜2011.6. 韓国国立江原大学
1.試験目的:千年硫黄−ゲル(硫黄+有用微生物)が作物に及ぶ効果を知るためのものである(肥効)
2.試験方法:
イ. 試験作物(品種):きゅうり
ロ.処理方法:茎葉散布
ハ.処理内容
<Example 3>
2011.4. ~ 2011.6. National Gangwon University, Korea Test purpose: To know the effects of millennial sulfur-gel (sulfur + useful microorganisms) on crops (fertilization effect)
2. Test method:
Test crop (variety): Cucumber b. Treatment method: foliage spray c. Processing content

Figure 0005444437
Figure 0005444437

ホ.生育特性   E. Growth characteristics

Figure 0005444437
*肥害:0−肥害無し、5 − 枯死
Figure 0005444437
* Fertilization: 0-no fertilization, 5-withering

ヘ.収穫特性   F. Harvest characteristics

Figure 0005444437
Figure 0005444437

ヘ.果実特性   F. Fruit characteristics

Figure 0005444437
Figure 0005444437

3.肥効試験結果:
完全任意配置法で40cm間隔で1株ずつ定植し、定植15日後、15日間隔で4回茎葉散布し、最後の散布後、17日後に生育及び収穫量を調査した結果、きゅうり生育の特性である草長が無処理区より処理区で有意的に高い傾向を表す。
きゅうりの収獲特性である果径の場合、収穫量が無処理区より有意的に高い傾向を示す。 また、きゅうり果実特性の場合、無処理区より千年硫黄−ゲル処理区で非定常果率が有意的に減少(71.8%)する傾向を表した。
4.試験担当者の意見:
千年硫黄−ゲル(硫黄+有用微生物)は、肥害が全然なくて無処理区に比べて有意的に優れた生育効果及び収獲特性を表すと判断される。
3. Fertilization test results:
Plants were planted one by one at 40 cm intervals using a completely arbitrary arrangement method, and foliage was sprayed four times at 15-day intervals, 15 days after planting, and the growth and yield were investigated 17 days after the last spraying. A certain plant length tends to be significantly higher in the treated area than in the untreated area.
In the case of fruit size, which is the harvest characteristic of cucumber, the yield tends to be significantly higher than that of the untreated area. In addition, in the case of cucumber fruit characteristics, the unsteady fruit rate was significantly decreased (71.8%) in the millennium-gel treated group than in the untreated group.
4). Opinion Opinion:
Sennen Sulfur-Gel (Sulfur + Useful Microorganisms) is judged to exhibit significantly better growth effects and harvest characteristics compared to the untreated plot without any fertilization.

<実施例4>
1.試験目的:千年硫黄−ゲル(硫黄+有用微生物)が作物に及ぶ効果を知るためのものである。(肥効)
2.試験方法:
イ.試験作物(品種):じゃがいも
ロ.処理方法:茎葉散布
ハ.処理内容
<Example 4>
1. Test purpose: To know the effects of millennial sulfur-gel (sulfur + useful microorganisms) on crops. (Fertilization effect)
2. Test method:
A. Test crop (variety): Potato b. Treatment method: foliage spraying c. Processing content

Figure 0005444437
Figure 0005444437

ホ.生育特性   E. Growth characteristics

Figure 0005444437
*肥害:0−肥害無し、5−枯死
Figure 0005444437
* Fertilization: 0-no fertilization, 5-death

ヘ.収穫特性   F. Harvest characteristics

Figure 0005444437
Figure 0005444437

3.肥効試験結果:
完全任意配置法により40cm間隔1株ずつ定植し、定植40日後、10日間隔で3回降雨により15日後、1回茎葉散布し、最後の散布後、17日後に生育及び収穫量を調査した結果、じゃがいも生育の特性である草長、葉長、葉幅、及びSPADは、無処理区より処理区で有意的に高い傾向を表した。
収穫量及び果長の場合にも無処理区より有意的に高い傾向(15.4%増収)を表す。
4.試験担当者の意見
千年硫黄−ゲルは、肥害が全然なく、無処理区に比べて有意的に優れた生育効果及び収獲特性を表すと判断される。
3. Fertilization test results:
The result of investigating the growth and yield after 17 days after the last spraying, after the last spraying, 15 days after the last spraying, after 15 days by raining 3 times every 10 days after planting, after planting at 40cm intervals one by one The plant length, leaf length, leaf width, and SPAD, which are characteristics of potato growth, showed a significantly higher tendency in the treated group than in the untreated group.
The yield and fruit length also show a significantly higher tendency (15.4% increase) than the untreated plot.
4). Opinion of the test personnel It is judged that millennial sulfur-gel has no fertilization and exhibits significantly better growth effects and harvest characteristics than the untreated plot.

<実施例5>
1.試験目的:千年硫黄−ゲル(硫黄+有用微生物)が作物に及ぶ効果を知るためのものである。(肥効)
2.試験方法:
イ.試験作物(品種):チシャ
ロ.処理方法:茎葉散布
ハ.処理内容
<Example 5>
1. Test purpose: To know the effects of millennial sulfur-gel (sulfur + useful microorganisms) on crops. (Fertilization effect)
2. Test method:
A. Test crop (variety): Chicharo. Treatment method: foliage spraying c. Processing content

Figure 0005444437
Figure 0005444437

ホ.生育特性   E. Growth characteristics

Figure 0005444437
Figure 0005444437

ヘ.収穫量   F. Yield

Figure 0005444437
Figure 0005444437

3.肥効試験結果:
完全任意配置法及び40cm間隔で1株ずつ定植し、定植21日後、基準量1,000倍及び倍量500倍を7日間隔で3回茎葉散布し、最後の散布14日後(2011.3.7)調査したところ、チシャの生育特性である葉長及び葉幅、葉重及び個重が有意的に優れて高く、SPAD も千年硫黄−ゲル処理区が無処理区より有意的に高く、チシャの「千年硫黄−ゲル」肥料処理区が無処理区よりさらに高い収穫量(34.67%増収)を表した。
4.試験担当者意見:
千年硫黄−ゲル処理区は、肥害が全然なく、無処理区に比べて有意的に優れた生育効果を表す。
3. Fertilization test results:
Planted one plant at a time with a completely arbitrary placement method and 40 cm intervals, 21 days after planting, 1,000 times the standard amount and 500 times the double amount were sprayed 3 times at 7-day intervals, and the last 14 days after spraying (2011.3.7) However, the leaf length and leaf width, leaf weight and individual weight, which are the characteristics of chisha, are significantly superior and high, and SPAD is also significantly higher in the millennium sulfur-gel treated group than in the untreated group. -"Gel" fertilizer treated area showed higher yield (34.67% increase) than untreated area.
4). Examiner opinion:
The millennium sulfur-gel treated zone has no fertilization and exhibits a significantly superior growth effect compared to the untreated zone.

<実施例6>
1.試験目的:千年硫黄−ゲル(硫黄+有用微生物)が作物に及ぶ効果を知るためのものである。(肥効)
2.試験方法:
イ.試験作物(品種):トマト
ロ.処理方法:茎葉散布
ハ.処理内容
<Example 6>
1. Test purpose: To know the effects of millennial sulfur-gel (sulfur + useful microorganisms) on crops. (Fertilization effect)
2. Test method:
A. Test crop (variety): Tomato b. Treatment method: foliage spray
C. Processing content

Figure 0005444437
Figure 0005444437

ホ.生育特性   E. Growth characteristics

Figure 0005444437
*肥害:0 − 肥害無し、5 − 枯死
Figure 0005444437
* Fertilization: 0-no fertilization, 5-withering

ヘ.収穫量   F. Yield

Figure 0005444437
Figure 0005444437

3.肥効試験結果:
40cm間隔で1株ずつ定植21日後、基準量1,000倍及び倍量500倍を7日間隔で3回茎葉散布し、14日後に2011年3月7日に調査したところ、トマトの生育特性である草長、葉長、葉幅、茎径が無処理区より千年硫黄−ゲルが有意的に優れ、 SPADも千年硫黄−ゲル処理区が無処理区より有意的に高く、収穫量においても「千年硫黄−ゲル」肥料処理区が無処理区より高い収穫量(14.50%増収)を表した。
4.試験担当者の意見:
千年硫黄−ゲル肥料は、肥害が全然なく、無処理区に比べて有意的に優れた生育効果を表すと判断される。
3. Fertilization test results:
After planting 21 days after planting one plant at 40 cm intervals, 1,000 times the standard amount and 500 times the double amount were sprayed 3 times at 7-day intervals, and 14 days later, on March 7, 2011, it was the growth characteristics of tomatoes The plant length, leaf length, leaf width, and stem diameter are significantly better for the millennium-gel than the untreated plot, and the SPAD is also significantly higher for the millennium-gel treated plot than the untreated plot. The “sulfur-gel” fertilizer treated area showed higher yield (14.50% increase) than the untreated area.
4). Opinion Opinion:
Sennen sulfur-gel fertilizer has no fertilization and is judged to exhibit a significantly superior growth effect compared to the untreated section.

本発明によると、硫黄を好適に適用し、防除防疫及び作物生育に寄与するので、産業上利用価値が高い。   According to the present invention, since sulfur is suitably applied and contributes to control and prevention and crop growth, the industrial utility value is high.

Claims (2)

EM原液、活性液、醗酵液を含む液状形態の有用微生物群(EM:Effective Microorganism)と、除毒されない硫黄粉末を1:1〜99:1の体積割合で混合した液状混合物を、収容する保存槽の内外部に、加熱装置を設けて、間接加熱で25〜60℃の温度に24時間保持するステップと、
除毒されない硫黄と有用微生物群の液状混合物を、撹拌装置で撹拌するステップと、を有する
ことを特徴とする防除防疫及び作物生育のために除毒されない硫黄と有用微生物群を液状混合する方法。
Preservation that contains a liquid mixture of EM (Effective Microorganism) containing EM stock solution, active solution and fermentation broth, and non-detoxified sulfur powder in a volume ratio of 1: 1 to 99: 1 A step of providing a heating device inside and outside the tank and maintaining the temperature at 25 to 60 ° C. for 24 hours by indirect heating;
Stirring a liquid mixture of non-detoxified sulfur and useful microorganisms with a stirrer, a method for liquid mixing sulfur and non-detoxified microorganisms for control and prevention and crop growth.
EM原液、活性液、醗酵液を含む液状形態の有用微生物群(EM:Effective Microorganism)と、除毒されない硫黄粉末を1:1〜99:1の体積割合で混合した液状混合物を、収容する保存槽の内外部に、加熱装置を設けて、間接加熱で25〜60℃の温度に24時間保持するステップと、
除毒されない硫黄と有用微生物群の液状混合物を、撹拌装置で撹拌するステップと、により製造された
ことを特徴とする防除防疫及び作物生育のために除毒されない硫黄と有用微生物群を液状混合した組成物。
Preservation that contains a liquid mixture of EM (Effective Microorganism) containing EM stock solution, active solution and fermentation broth, and non-detoxified sulfur powder in a volume ratio of 1: 1 to 99: 1 A step of providing a heating device inside and outside the tank and maintaining the temperature at 25 to 60 ° C. for 24 hours by indirect heating;
A liquid mixture of non-detoxified sulfur and useful microorganisms was stirred with a stirring device, and the non-detoxified sulfur and useful microorganisms were mixed in a liquid mixture for control and prevention and crop growth. Composition.
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