JP5443488B2 - 微生物に対する受容体アレイを形成する方法 - Google Patents
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Description
電子ビーム・リソグラフィを用いて高解像度ナノテンプレートを作製した。ポリ(メチルメタクリレート)(Poly(methyl methacrylate):PMMA)レジストでコーティングしたシリコン・ウエハを、ドイツ、ドルトムントのRaith GmbH製造のe−ライン電子ビーム・リソグラフィ・システム(電圧:20キロボルト(kV)、アパーチャ:10μm、ビーム電流:29ピコアンペア(pA))を用いて露光した。PMMAレジストを、1:3の割合のメチルイソブチルケトン(methyl isobutyl ketone:MIBK):イソプロパノールの溶液中で30秒(s)現像し、イソプロパノール中に1分(min)浸漬し、N2気流を吹きつけて、乾燥させた。エッチング用の前駆物質として六フッ化硫黄(SF6)および側壁のパッシベーション用にオクタフルオロシクロブタン(C4F8)(フランス、アヌシーのAlcatel Vacuum Technology France製造)を使用するバランスの取れたプロセスで、低エッチング速度の反応性イオン・エッチャ(Alcatel Vacuum Technology France、アヌシー、フランス)を用いて25秒間エッチングすることによって、シリコン基板内にPMMAパターンを転写した。
ミシガン州ミッドランドのダウコーニングから市販されているSylgard(登録商標)184ポリジメチルシロキサン(polydimethylsiloxane:PDMS)エラストマーを、ペトリ皿に入れ、60℃で少なくとも24時間硬化した。各エラストマーの、ペトリ皿に接触している方の側を約100マイクロリットル(μl)の抗体溶液で45分間インク付けした。Anti−fd Bacteriophage(米国ミズーリ州セントルイスのシグマが販売するB7786)を、リン酸緩衝食塩水(phosphate buffered saline:PBS)(シグマが販売するA7906)中に0.1ミリグラム/ミリリットル(mg/ml)の濃度で用いた。インク付けの後、PBSおよび脱イオン水を用いてエラストマーをリンスし、N2気流を約30秒吹きつけ、乾燥した。
サブトラクティブ・プリンティング技術の詳細は、たとえば、Coyer,S.R.,Garcia,A.J.& Delamarche,“E.Facile preparation of complex protein architectures with sub−100 nm resolution on surfaces”,Angew.Chem.Int.Ed.vol.46,p.1−5(2007)の中で以前に発表されており、これを参照によって本明細書に組み込む。簡単に説明すると、米国ケンタッキー州フローレンスのTechnics Plasma 100−E製造のデバイスを用いて、酸素プラズマ処理を200ワットで60秒間行って、シリコン基板およびナノテンプレートを清浄にした。エラストマーをナノテンプレートに15秒接触させることによって、均一にインク付けされたエラストマー上のタンパク質を選択された領域で除去し、その後、手でエラストマーをナノテンプレートから取り外した。30秒間のプリンティング・ステップを用いてエラストマーから最終基板にタンパク質のパターンを転写した。エラストマーをナノテンプレート/基板上に手で置き、ピンセットで軽度の圧力を加えると、エラストマーとナノテンプレート/基板の間の密着が生じた。ナノテンプレートは、再利用する前に、酸素プラズマでの処理を繰り返し行うことによって有機材料を除去して清浄にした。
スイス、ヌーシャテルのNanosensorsが販売している174〜191キロヘルツ(kHz)のシリコン・カンチレバーを用いてタッピング・モードで操作されたNanoscope Dimension 3000(米国カリフォルニア州サンタバーバラのDigital Instrumentsが販売)を用いて、原子間力顕微鏡(AFM)像を得た。NanoScope 6.12r1ソフトウェアを用いて、AFM像を平坦化し、表示し、分析した。
Barbas,C.F.,Burton,D.R.,Scott,J.K.,Phage Display:A Laboratory Manual.Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,2001に記載されている標準ファージ方法を用いて、宿主細菌である大腸菌(ER2738 NEB)の中でM13バクテリオファージ・ストック(NEB)を増幅した。この文献を参照によって本明細書に組み込む。簡単に説明すると、細菌を終夜培養して1:100に希釈したものにファージ・ストック(1×1012pfu/ml)を添加し、37℃で振盪させながら5.5時間インキュベートした。遠心分離によって細菌からファージを分離し、4℃での終夜に亘るポリエチレングリコール/塩化ナトリウムの沈殿によって濃縮し、続いて遠心分離した。得られたファージを透析して過剰な塩を除去し、確実に適正なpHにした。
トリス緩衝食塩水(tris−buffered saline:TBS)および0.1重量%のTween−20(シグマ・アルドリッチが販売)の溶液中の5mlのファージ・ストックを、サブトラクティブ・プリンティングされた基板とともに1時間インキュベートし、続いてTBST(TBS+Tween−20)、TBS、および水(18.2メガオームの電気抵抗を有する)を用いて、穏やかにではあるが徹底的に洗浄し、圧縮窒素で乾燥した。AFM分析の前に、試料を終夜真空デシケータ内に置いた。
Claims (10)
- M13バクテリオファージに対する受容体アレイを形成する方法であって、
第1の基板上にストラクチャのアレイをパターニングして、前記第1の基板の表面上にテンプレートを形成するステップであって、前記ストラクチャ間の平均ピッチが、5〜700マイクロメートルである、前記形成するステップと、
第2の基板のフェイス面にタンパク質受容体材料を付けるステップであって、前記第2の基板がエラストマーである、前記付けるステップと、
前記テンプレートに前記第2の基板の前記フェイス面を接触させて、前記第2の基板から前記タンパク質受容体材料の一部を除去するステップであって、当該接触及び当該除去によって、前記第2の基板上に受容体アレイを形成する、前記除去するステップと、
前記第2の基板上の前記受容体アレイに対象基板を接触させて、前記対象基板上に当該受容体アレイのパターンを転写するステップであって、当該転写された受容体アレイの前記パターンが、平均横寸法が200nm×200nm以下である複数の受容体部位を備えている、前記転写するステップと、
107〜109プラーク形成単位(pfu)/mlの濃度を有するM13バクテリオファージ含有溶液に前記受容体アレイのパターンが転写された前記対象基板を接触させて前記受容体アレイに前記M13バクテリオファージの一部を転写するステップであって、前記受容体アレイ中の受容体部位の過半数が単一のM13バクテリオファージに占有される、前記転写するステップと
を含む、前記方法。 - 前記エラストマーがポリジメチルシロキサンを含む、請求項1に記載の方法。
- 前記受容体アレイに転写された前記M13バクテリオファージを、マイクロ流体プローブを用いて、操作、回収、または染色するステップをさらに含む、請求項1又は2に記載の方法。
- 前記マイクロ流体プローブが、溶離液、別のタンパク質受容体を含む液体、前記受容体アレイに転写された前記M13バクテリオファージに付着可能なリガンドを含む液体、またはこれら液体の少なくとも1つを含む組み合わせを、前記基板に供給する、請求項3に記載の方法。
- 前記マイクロ流体プローブが、蛍光標識した受容体を含む液体を前記基板に供給する、請求項3に記載の方法。
- 前記受容体アレイに転写された前記M13バクテリオファージが、免疫蛍光染色を用いて検出される、請求項1〜5のいずれか一項に記載の方法。
- 前記マイクロ流体プローブが、前記受容体アレイに転写された前記M13バクテリオファージ上のタンパク質コーティングに結合可能な蛍光標識した抗体を含む液体を前記対象基板に供給する、請求項3に記載の方法。
- 前記受容体アレイに転写された前記M13バクテリオファージを、溶離を用いて回収するステップをさらに含む、請求項1〜7のいずれか一項に記載の方法。
- 前記方法は、前記M13バクテリオファージの前記転写された一部をM13バクテリオファージアレイに配置し、前記M13バクテリオファージアレイの方向が流体流を用いて調整される、請求項1〜8のいずれか一項に記載の方法。
- 前記対象基板が、スライドガラス、自然生成した二酸化シリコンもしくはこれより厚い二酸化シリコン・レイヤでコーティングされたシリコン・ウエハ、または前記コーティングされたシリコン・ウエハの一部である、請求項1〜9のいずれか一項に記載の方法。
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US12/195,577 US8680023B2 (en) | 2008-08-21 | 2008-08-21 | Methods for screening and arraying microrganisms such as viruses using subtractive contact printing background |
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PCT/IB2009/053098 WO2010020893A1 (en) | 2008-08-21 | 2009-07-16 | Methods for screening and arraying microrganisms such as viruses using subtractive contact printing background |
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CA2956645A1 (en) * | 2003-07-12 | 2005-03-31 | David A. Goldberg | Sensitive and rapid biodetection |
US7341841B2 (en) * | 2003-07-12 | 2008-03-11 | Accelr8 Technology Corporation | Rapid microbial detection and antimicrobial susceptibility testing |
US20120077206A1 (en) | 2003-07-12 | 2012-03-29 | Accelr8 Technology Corporation | Rapid Microbial Detection and Antimicrobial Susceptibility Testing |
WO2011109368A2 (en) | 2010-03-01 | 2011-09-09 | Cornell University | Patterning of biomaterials using fluorinated materials and fluorinated solvents |
FR2964391B1 (fr) | 2010-09-03 | 2014-04-11 | Centre Nat Rech Scient | Biopuces pour l'analyse de la dynamique de molecules d'acide nucleique |
ES2551922T3 (es) | 2011-03-07 | 2015-11-24 | Accelerate Diagnostics, Inc. | Sistemas rápidos de purificación celular |
US10254204B2 (en) | 2011-03-07 | 2019-04-09 | Accelerate Diagnostics, Inc. | Membrane-assisted purification |
US9677109B2 (en) | 2013-03-15 | 2017-06-13 | Accelerate Diagnostics, Inc. | Rapid determination of microbial growth and antimicrobial susceptibility |
EP3011061B1 (en) * | 2013-06-21 | 2021-12-29 | The Johns Hopkins University | Virion display array for profiling functions and interactions of human membrane proteins |
US10732189B2 (en) | 2015-01-14 | 2020-08-04 | Bio-Rad Europe Gmbh | Blood analysis systems and methods |
EP3248012B8 (en) | 2015-01-23 | 2019-11-13 | Bio-Rad Laboratories, Inc. | Immunoblotting systems and methods |
US10023895B2 (en) | 2015-03-30 | 2018-07-17 | Accelerate Diagnostics, Inc. | Instrument and system for rapid microogranism identification and antimicrobial agent susceptibility testing |
US10253355B2 (en) | 2015-03-30 | 2019-04-09 | Accelerate Diagnostics, Inc. | Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing |
WO2019219757A1 (de) * | 2018-05-15 | 2019-11-21 | Albert-Ludwig-Universität Freiburg | Mikroarray-transformer |
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EP1691196B1 (en) | 2003-09-25 | 2012-12-26 | Toyama Prefecture | Microwell array chip and its manufacturing method |
WO2006055925A2 (en) | 2004-11-19 | 2006-05-26 | Swiss Federal Institute Of Technology | Microarrays for analyte detection |
ES2362797T3 (es) | 2005-08-31 | 2011-07-13 | Northwestern University | Nanoarreglos de partículas biológicas, métodos para la fabricación de los mismos. |
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