JP5345751B2 - Preventive or ameliorating agent for anemia for domestic animals - Google Patents

Description

The present invention relates to a preventive or ameliorating agent for livestock animals containing difructose anhydride III (hereinafter sometimes referred to as DFAIII) and / or difructose anhydride IV (hereinafter also referred to as DFAIV) as an active ingredient. Is.

Newborn pups are prone to anemia, and anemia of pups is listed as one of the typical examples of dysfunction caused by mineral deficiency. Since the newborn pups just after birth are difficult to maintain a constant body temperature, the function of iron that promotes the hematopoietic function is important. Iron plays an important role in improving the hematopoietic function as a component of hemoglobin. It is known that when newborn pups cannot maintain the homeostasis of body temperature, harmful microorganisms easily enter the pups and the survival rate of the pups decreases due to the occurrence of diarrhea, pneumonia and the like.

According to a report on the occurrence of debilitating diseases of Japanese black cattle cattle, anemia was observed in calves that seemed to be healthy within 59 days of age, and the serum iron concentration decreased especially at 30-59 days of age. Was. Since the disease occurs frequently at this time, this is considered to be caused by a decrease in anti-disease due to anemia.

In recent years, with the practical application of fertilized egg transplantation technology, twin production has great expectations as a measure for expanding beef resources. However, it is reported that twin cattle have a low survival rate after birth, and one of the causes is that twin cattle have a high incidence of anemia. Decreased hematopoietic function has been observed immediately after birth, with twin calves being more than single calves, calves born from first calves more than calves born from heifers, and calves more than female calves.

In newborn piglets, it is known that when the piglet starts sucking colostrum, the components of the absorbed colostrum enter the blood and the circulating plasma volume increases, resulting in blood dilution and anemia. ing. The growth rate of suckling piglets is very fast compared to other young animals, so that the number of red blood cells and hemoglobin (hemoglobin) in the blood can be maintained at normal levels in response to the rapid increase in circulating plasma volume. A large amount of iron is required. However, mother pigs are kept in a closed house-keeping system, and trace elements such as iron that were originally ingested from soil and grass are scarce, and the amount of iron in milk is reduced. Piglets are prone to anemia.

In this way, anemia in newborn calves and newborn piglets is one of the typical dysfunctions due to mineral deficiencies, and calves and piglets born in anemia have a lower survival rate and subsequent growth rate. Furthermore, the decrease in hematopoietic function also adversely affects the immune function of newborn pups.

Currently, the administration of iron is recommended as a method for improving the hematopoietic function of newborn calves and newborn piglets. However, excessive administration of iron adversely affects the growth of harmful microorganisms in the gastrointestinal tract and tissue damage due to the promotion of peroxidation. To develop a new method.

For example, Patent Document 1 discloses that by administering lactoferrin and iron to a newborn calf, early improvement can be obtained in improving the hematopoietic function and preventing anemia of the newborn calf, and plasma iron immediately after administration. It describes that side effects such as toxicity are alleviated by suppressing a rapid increase in content. However, lactoferrin alone does not improve the hematopoietic function and prevent anemia, and it is necessary to use lactoferrin and iron together, so this technique does not eliminate the risk of side effects on digestive tissues caused by iron administration . In addition, lactoferrin is vulnerable to moisture, heat, acid, enzymes, etc., and will be decomposed in the stomach if taken as it is, so it is stable over a wide pH range for a long time and has heat resistance. Processing is required (see, for example, Patent Document 2).

Moreover, there is a report that peptide iron has an effect of improving anemia of piglets (see, for example, Non-Patent Document 1), and it is widely used as a feed additive at home and abroad. Peptidized minerals are thought to be absorbed directly into the body from the peptide absorption site in the small intestine. Compared to inorganic minerals, it has better absorbability, less excretion outside the body, better stability, etc. It has the characteristics. However, it has also been pointed out that it is not desirable to add more than necessary to eliminate deficiency, since the balance of mineral nutrition is important and excess is as bad as deficiency.

On the other hand, with regard to DFAIII and DFAIV, which are oligosaccharides formed by binding two fructose molecules, it has already been disclosed that DFAIII and / or DFAIV is effective in preventing and treating calcium deficiency and edema in domestic animals ( For example, see Patent Document 3). However, it does not describe that it promotes iron absorption and has an effect on the prevention or improvement of anemia.
Journal of Animal Science, 1978, Vol. 47, No. 5, p1135 Japanese Patent Laid-Open No. 08-289737 Japanese Patent Laid-Open No. 07-304798 JP 2004-329110 A

In view of the present state of the art, the present invention is a highly safe anemia-preventing agent or ameliorating agent for livestock animals that can more efficiently promote iron absorption, enhance hematopoietic function, and improve anemia. And the purpose of developing a feed containing the same.

The present invention has been made in order to solve the above-mentioned problems, and the present inventors have derived from natural products having an effect of promoting iron absorption and improving anemia of newborn dairy animals such as newborn calves and newborn piglets. As a result of diligent research on these materials, DFAIII, an oligosaccharide derived from inulin, and DFAIV, an oligosaccharide derived from levan, have a significant effect on promoting iron absorption and improving anemia in newborns. As a result, the present invention has been completed.

That is, the present invention relates to a preventive or ameliorating agent for livestock animals containing DFAIII and / or DFAIV as an active ingredient, and also relates to a feed for livestock animals.

DFAIII (difructose anhydride III) used as an active ingredient in the present invention is a disaccharide in which two molecules of fructose are linked by 1, 2 'and 2, 3' (di-D-fructofuranose-1, 2 ': 2, 3 'dianhydride). In addition, DFA IV (difructose anhydride IV) used as an active ingredient is a disaccharide (di-D-fructofranose-2, 6 ′: 6, 2) in which two molecules of fructose are bound at 2, 6 ′ and 6, 2 ′. 'dianhydride). In addition, as an active ingredient, DFAIII and / or DFAIV refined product, crude refined product, intermediate product produced in the production process, the same effluent, these processed products (concentrate, pasted product, dried product, diluted product, At least one selected from suspensions and emulsions is included.

In the present invention, DFAIII and / or DFAIV is used as an active ingredient, and if desired, iron citrate, sodium iron succinate, DL-threonine iron, ferrous fumarate, iron sulfate (dried), An iron agent recognized as a feed additive such as peptide iron can be used in combination. In consideration of the balance with other minerals, the dose is preferably as small as possible within a range that does not become excessive, but there is no particular limitation.

According to the present invention, an anemia-preventing or ameliorating agent for livestock animals and a feed containing the same are prepared using the above-mentioned active ingredients, and the dosage of DFAIII and / or DFAIV is as follows: The dose is 0.01 to 2.0 g, preferably 0.05 to 0.5 g per kg of the animal body weight.

Moreover, the administration form may be administered as it is, or it may be added to colostrum, raw milk, milk substitute or artificial milk for administration.

The anemia preventive or ameliorating agent of the present invention and feed containing the same can be applied to various livestock such as buffalo, sheep, goats, horses, dogs, cats, etc. in addition to cattle and pigs.

According to the present invention, it is possible to improve anemia of newborn kittens such as newborn calves and newborn piglets, to reduce the mortality rate, to enhance subsequent growth, and to make a great contribution to livestock management.

Examples of the present invention are shown below, but the present invention is not limited to the following examples.

Eighteen newborn Holstein calves were divided into three groups of 6 groups, a control group, a DFAIII group, and a DFAIV group. After feeding colostrum for 7 days after birth, the control group is given 2 L of a commercially available 15% milk substitute solution, and the DFA III and DFA IV groups are mixed with 100 ml of a 5% solution of DFA III or DFA IV, respectively. Gave. Milk replacers were fed twice a day, up to 4 weeks of age, and commercially available calf feed and water were freely consumed. At the age of 4 weeks of age, blood was collected from the jugular vein of calves, and the serum iron concentration, hematocrit value and hemoglobin concentration in the blood were measured. Serum iron was measured by a ferrozine colorimetric method, and hematocrit value and hemoglobin concentration in blood were measured by QBCV Autoread (IDEX Laboratory Co., Ltd.). The results are shown in Table 1.

Holstein calves to which DFAIII and DFAIV were administered had higher serum iron levels, hematocrit values and hemoglobin levels and improved hematopoietic function compared to calves that had not been administered.

Sixty six newborn piglets were divided into three groups, a control group, a DFAIII group, and a DFAIV group. The piglets were allowed to live with the mother pigs, and commercially available feed and water for suckling piglets were freely consumed. In DFAIII and DFAIV groups, 20 ml of a 5% solution of DFAIII or DFAIV was orally administered by gavage once a day until 5 days of age. The survey item was survival rate (%) up to 24 days of age. The results are shown in FIG.

The piglets that received DFAIII and DFAIV had an increased survival rate compared to the piglets that did not.

From the above results, it was revealed that administration of DFAIII and / or DFAIV to newborn calves and newborn piglets improved the hematopoietic function and increased the survival rate.

It is the graph which showed the effect which administration of DFAIII and DFAIV has on the survival rate (%) of a newborn piglet.

Claims (2)

A milk substitute for preventing or improving anemia in newborn calves, characterized by adding difructose anhydride III and / or difructose anhydride IV and not using an iron component in combination . A method for preventing or improving anemia in a newborn calf, characterized by adding difructose anhydride III and / or difructose anhydride IV and administering a milk substitute not using an iron component to a newborn calf.

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