JP5276004B2 - アンギオゲニンの調製方法 - Google Patents
アンギオゲニンの調製方法 Download PDFInfo
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- JP5276004B2 JP5276004B2 JP2009535525A JP2009535525A JP5276004B2 JP 5276004 B2 JP5276004 B2 JP 5276004B2 JP 2009535525 A JP2009535525 A JP 2009535525A JP 2009535525 A JP2009535525 A JP 2009535525A JP 5276004 B2 JP5276004 B2 JP 5276004B2
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- Prior art keywords
- angiogenin
- enriched fraction
- cation exchange
- mobile phase
- resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/14—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
- A23C9/146—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by ion-exchange
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/20—Milk; Whey; Colostrum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/515—Angiogenesic factors; Angiogenin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Public Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Vascular Medicine (AREA)
- Toxicology (AREA)
- Epidemiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Heart & Thoracic Surgery (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cardiology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Description
(a)乳製品をカチオン交換樹脂に接触させることと、
(b)移動相を用いてアンギオゲニン富化分画をカチオン交換樹脂から溶出させることと、及び
(c)溶出させたアンギオゲニン富化分画を回収することと、を含み、前記乳製品は段階(a)に先立ちカチオン交換クロマトグラフィーに付さない方法を提供する。
10cm長のカラムにSPセファロースビッグビーズ(GEヘルスケア)をカラム中の全ベッド体積が29.7Lとなるように充填した。ウシスキムミルクを線流量331cm/h(スキムミルク34L/樹脂(1L)/時)で2時間カラムに流通させ、適用したスキムミルクの体積がカラムに充填した樹脂の68倍となるようにした。
Claims (24)
- 乳製品からのアンギオゲニン富化分画の調製方法であって、該方法は、
(a)乳製品をカチオン交換樹脂に接触させることと、
(b)0.30〜0.38M塩化ナトリウム(1.75〜2.25%w/v)又はこれのイオン強度等価物の移動相を用いてアンギオゲニン低減化ラクトペルオキシダーゼ分画をカチオン交換カラムから溶出させることと、
(c)移動相を用いてアンギオゲニン富化分画をカチオン交換樹脂から溶出させることと、
(d)溶出させたアンギオゲニン富化分画を回収することと、を含み、前記乳製品は段階(a)に先立ちカチオン交換クロマトグラフィーに付さない方法。 - 前記乳製品は全乳、スキムミルク及びバターミルクからなる群から選択される、請求項1に記載の方法。
- 前記カチオン交換樹脂に接触させる乳製品の量は、16カラム体積〜600カラム体積である、請求項1又は2に記載の方法。
- 前記カチオン交換樹脂に接触させる乳製品の量は、16カラム体積〜150カラム体積である、請求項1又は2に記載の方法。
- 前記カチオン交換樹脂に接触させる乳製品の量は、68カラム体積である、請求項1又は2に記載の方法。
- 前記アンギオゲニン低減化ラクトペルオキシダーゼ分画は、0.34M塩化ナトリウム(2.0%w/v)又はこれのイオン強度等価物を含み、pHが6.5である移動相を用いて溶出させる、請求項1に記載の方法。
- 前記アンギオゲニン富化分画の溶出に先立ち、カチオン交換樹脂を低イオン強度(<0.34Mの塩又はその等価物)のバッファー又は水で洗うことにより、カラムに残存する乳製品を除去する、請求項1〜6のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画は0.38〜1.5M塩化ナトリウム(2.25〜8.7%w/v)又はこれのイオン強度等価物を含む移動相を用いて溶出させる、請求項1〜7のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画は0.38〜0.59M塩化ナトリウム(2.25〜3.5%w/v)又はこれのイオン強度等価物を含む移動相を用いて溶出させる、請求項1〜8のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画は0.43M塩化ナトリウム(2.5%w/v)又はこれのイオン強度等価物を含む移動相を用いて溶出させる、請求項1〜9のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画はpH4.0〜9.0を有する移動相を用いて溶出させる、請求項1〜10のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画はpH5.5〜7.5を有する移動相を用いて溶出させる、請求項1〜11のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画はpH6.5を有する移動相を用いて溶出させる、請求項1〜12のいずれか一項に記載の方法。
- 前記カチオン交換樹脂はセファローズビーズを含むものである、請求項1〜13のいずれか一項に記載の方法。
- 前記セファローズビーズの粒径は45〜900μmである、請求項14に記載の方法。
- 前記セファローズビーズの粒径は90〜300μmである、請求項14又は15に記載の方法。
- 段階(a)の間、前記カチオン交換樹脂を流量6〜90L/L(樹脂)/時に付す、請求項1〜16のいずれか一項に記載の方法。
- 接触流量は6〜50L/L(樹脂)/時である、請求項17に記載の方法。
- 接触流量は33L/L(樹脂)/時である、請求項17又は18に記載の方法。
- 前記方法は連続方法又はバッチ方法である、請求項1〜19のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画はアンギオゲニンを2%w/w以上含む、請求項1〜20のいずれか一項に記載の方法。
- 前記アンギオゲニン富化分画は、組成物中の非アンギオゲニンタンパク質及び/又は塩の量を低減するために更に処理される、請求項1〜21のいずれか一項に記載の方法。
- 請求項1〜22のいずれかの方法によりアンギオゲニン富化分画を得、得られたアンギオゲニン富化分画を食品物質、栄養補助食品又は幼児食サプリメントに配合することを特徴とするアンギオゲニン富化分画を含有する食品物質、栄養補助食品又は幼児食サプリメントの製造法。
- 請求項1〜22のいずれかの方法によりアンギオゲニン富化分画を得、得られたアンギオゲニン富化分画及び医薬的に許容される担体を配合することを特徴とするアンギオゲニン富化分画含有医薬組成物の製造法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2006906288 | 2006-11-10 | ||
AU2006906288A AU2006906288A0 (en) | 2006-11-10 | Process for the preparation of angiogenin | |
PCT/AU2007/001719 WO2008055310A1 (en) | 2006-11-10 | 2007-11-09 | Process for the preparation of angiogenin |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2010508827A JP2010508827A (ja) | 2010-03-25 |
JP2010508827A5 JP2010508827A5 (ja) | 2010-09-24 |
JP5276004B2 true JP5276004B2 (ja) | 2013-08-28 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009535525A Expired - Fee Related JP5276004B2 (ja) | 2006-11-10 | 2007-11-09 | アンギオゲニンの調製方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US8551547B2 (ja) |
EP (1) | EP2117330B1 (ja) |
JP (1) | JP5276004B2 (ja) |
CN (1) | CN101626693B (ja) |
AU (1) | AU2007317200B8 (ja) |
CA (1) | CA2702645C (ja) |
NZ (1) | NZ577195A (ja) |
WO (1) | WO2008055310A1 (ja) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5717625B2 (ja) | 2008-05-14 | 2015-05-13 | アグリカルチャー ヴィクトリア サービス ピーティーワイ エルティーディーAgriculture Victoria Services Pty Ltd | アンギオゲニン濃縮ミルク分画 |
MX2010012437A (es) | 2008-05-14 | 2011-06-01 | Agriculture Victoria Serv Pty | Uso de angiogenina o agonistas de angiogenina para tratar enfermedades y trastornos. |
EP2307044A4 (en) * | 2008-05-14 | 2011-10-05 | Agriculture Victoria Serv Pty | ORAL ADMINISTRATIVE DOSAGE FORMS COMPRISING ANGIOGENIN AND USES THEREOF |
JP2013511261A (ja) * | 2009-11-18 | 2013-04-04 | マリー ゴールバーン シーオー−オペレイティブ シーオー.リミテッド | 組換え微生物 |
WO2011113100A1 (en) * | 2010-03-17 | 2011-09-22 | Murray Goulburn Co-Operative Co. Limited | Method of treating a bone disorder |
AU2013204740C1 (en) | 2012-05-10 | 2015-10-01 | Agriculture Victoria Services Pty Ltd | Methods of treating cancer using angiogenin or an angiogenin agonist |
CA2879962C (en) * | 2012-07-31 | 2018-11-20 | Megmilk Snow Brand Co., Ltd. | A drink of the combination of angiogenin and lactoperoxidase for use in prevention or treatment of bone disease |
BR112015002049A2 (ja) * | 2012-07-31 | 2018-06-12 | Megmilk Snow Brand Co., Ltd. | A drink and a manufacturing method for the same |
MY167580A (en) * | 2012-07-31 | 2018-09-20 | Megmilk Snow Brand Co Ltd | Novel fermented milk product and method for producing the same |
JP6203724B2 (ja) * | 2012-07-31 | 2017-09-27 | 雪印メグミルク株式会社 | 骨疾患の予防又は治療用タンパク質素材及びその製造方法 |
CA2879992C (en) | 2012-07-31 | 2020-08-25 | Megmilk Snow Brand Co., Ltd. | Novel fermented milk product and method for producing the same |
AU2013204858B2 (en) * | 2012-10-08 | 2015-06-25 | Saputo Dairy Australia Pty Limited | Improved process for purifying milk proteins and products thereof |
JP2016152809A (ja) * | 2016-04-05 | 2016-08-25 | 雪印メグミルク株式会社 | 飲料及びその製造方法 |
JP6562958B2 (ja) * | 2017-02-28 | 2019-08-21 | 雪印メグミルク株式会社 | 発酵乳類及びその製造方法 |
JP6562957B2 (ja) * | 2017-02-28 | 2019-08-21 | 雪印メグミルク株式会社 | 発酵乳類及びその製造方法 |
JP6562956B2 (ja) * | 2017-02-28 | 2019-08-21 | 雪印メグミルク株式会社 | 飲料及びその製造方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
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KR930008447B1 (ko) * | 1987-10-23 | 1993-09-04 | 쉐링 코포레이션 | 단백질 정제방법 |
FR2623509B1 (fr) | 1987-11-19 | 1990-04-20 | Centre Nat Rech Scient | Proteine de 17 kd a action angiogenique, son procede d'isolement a partir de lait de mammiferes, compositions therapeutiques la contenant, procede de detection et/ou de dosage et reactifs immunologiques de detection et de dosage des angiogenines de mammiferes, de leurs homologues et de leurs fragments |
NZ249235A (en) * | 1992-01-15 | 1995-12-21 | Campina Melkunie Bv | Isolating lactoperoxidase or lactoferrin from milk and/or milk derivatives |
JP3112637B2 (ja) | 1994-09-30 | 2000-11-27 | 雪印乳業株式会社 | 骨強化剤 |
JP2974604B2 (ja) * | 1996-01-23 | 1999-11-10 | 雪印乳業株式会社 | 塩基性タンパク質組成物、塩基性ペプチド組成物及びその利用 |
RU2110066C1 (ru) | 1996-12-27 | 1998-04-27 | Московский государственный университет прикладной биотехнологии | Способ количественного определения ангиогенина в биологическом сырье |
NL1005677C2 (nl) | 1997-03-27 | 1998-09-29 | Campina Melkunie Bv | Werkwijze voor het winnen van groeifactoren, of een samenstelling die één of meer groeifactoren bevat, uit melk of een derivaat daarvan. |
RU2204262C2 (ru) | 2001-07-06 | 2003-05-20 | Московский государственный университет прикладной биотехнологии | Способ получения биологически активного белкового концентрата, обогащенного панкреатической рибонуклеазой а, ангиогенином и лизоцимом, из молочного ультрафильтрата |
JP4291967B2 (ja) * | 2001-11-08 | 2009-07-08 | 雪印乳業株式会社 | 皮膚コラーゲン産生促進剤 |
EP1529054A4 (en) * | 2001-11-27 | 2005-12-28 | Univ Washington | THERAPEUTIC PROTEIN AND TREATMENTS |
JP4698935B2 (ja) * | 2003-05-07 | 2011-06-08 | 雪印乳業株式会社 | 皮膚コラーゲン産生促進剤 |
WO2004106491A2 (en) * | 2003-05-22 | 2004-12-09 | The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Angiogenin-based hiv-1 therapies |
GB0425625D0 (en) | 2004-11-22 | 2004-12-22 | Royal College Of Surgeons Ie | Treatment of disease |
US20060251739A1 (en) * | 2005-05-04 | 2006-11-09 | National Yang-Ming University | Composition and method for treating autoimmune disease and mucosal disorder |
-
2007
- 2007-11-09 CN CN200780041515.4A patent/CN101626693B/zh not_active Expired - Fee Related
- 2007-11-09 NZ NZ577195A patent/NZ577195A/en unknown
- 2007-11-09 AU AU2007317200A patent/AU2007317200B8/en active Active
- 2007-11-09 US US12/447,889 patent/US8551547B2/en not_active Expired - Fee Related
- 2007-11-09 EP EP07815522.3A patent/EP2117330B1/en not_active Not-in-force
- 2007-11-09 WO PCT/AU2007/001719 patent/WO2008055310A1/en active Application Filing
- 2007-11-09 CA CA2702645A patent/CA2702645C/en not_active Expired - Fee Related
- 2007-11-09 JP JP2009535525A patent/JP5276004B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2702645C (en) | 2018-01-02 |
NZ577195A (en) | 2011-12-22 |
EP2117330A1 (en) | 2009-11-18 |
AU2007317200B8 (en) | 2012-02-02 |
JP2010508827A (ja) | 2010-03-25 |
WO2008055310A1 (en) | 2008-05-15 |
CN101626693B (zh) | 2014-05-14 |
AU2007317200A8 (en) | 2012-02-02 |
US8551547B2 (en) | 2013-10-08 |
AU2007317200A1 (en) | 2008-05-15 |
WO2008055310A8 (en) | 2008-09-04 |
AU2007317200B2 (en) | 2011-12-08 |
EP2117330B1 (en) | 2017-07-26 |
CN101626693A (zh) | 2010-01-13 |
EP2117330A4 (en) | 2011-05-04 |
CA2702645A1 (en) | 2008-05-15 |
US20100136172A1 (en) | 2010-06-03 |
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