JP5258084B2 - タンパク質間相互作用の検出方法 - Google Patents

タンパク質間相互作用の検出方法 Download PDF

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Publication number
JP5258084B2
JP5258084B2 JP2008000789A JP2008000789A JP5258084B2 JP 5258084 B2 JP5258084 B2 JP 5258084B2 JP 2008000789 A JP2008000789 A JP 2008000789A JP 2008000789 A JP2008000789 A JP 2008000789A JP 5258084 B2 JP5258084 B2 JP 5258084B2
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JP
Japan
Prior art keywords
protein
domain
amino acid
acid sequence
tlucc
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Expired - Fee Related
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JP2008000789A
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English (en)
Japanese (ja)
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JP2009159889A (ja
JP2009159889A5 (enExample
Inventor
岳昌 小澤
アワイス ムハンマド
研二 三浦
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Priority to JP2008000789A priority Critical patent/JP5258084B2/ja
Priority to PCT/JP2009/000033 priority patent/WO2009087967A1/en
Publication of JP2009159889A publication Critical patent/JP2009159889A/ja
Publication of JP2009159889A5 publication Critical patent/JP2009159889A5/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
JP2008000789A 2008-01-07 2008-01-07 タンパク質間相互作用の検出方法 Expired - Fee Related JP5258084B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008000789A JP5258084B2 (ja) 2008-01-07 2008-01-07 タンパク質間相互作用の検出方法
PCT/JP2009/000033 WO2009087967A1 (en) 2008-01-07 2009-01-07 Method for detecting a protein-protein interaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2008000789A JP5258084B2 (ja) 2008-01-07 2008-01-07 タンパク質間相互作用の検出方法

Publications (3)

Publication Number Publication Date
JP2009159889A JP2009159889A (ja) 2009-07-23
JP2009159889A5 JP2009159889A5 (enExample) 2011-03-17
JP5258084B2 true JP5258084B2 (ja) 2013-08-07

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JP2008000789A Expired - Fee Related JP5258084B2 (ja) 2008-01-07 2008-01-07 タンパク質間相互作用の検出方法

Country Status (2)

Country Link
JP (1) JP5258084B2 (enExample)
WO (1) WO2009087967A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12529699B2 (en) 2020-04-17 2026-01-20 Regeneron Pharmaceuticals, Inc Detection assays for coronavirus neutralizing antibodies

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4849698B2 (ja) * 2009-05-29 2012-01-11 国立大学法人 東京大学 タンパク質間相互作用の高感度検出方法
PL450655A1 (pl) * 2024-12-20 2026-06-22 Echia Lab Spółka Z Ograniczoną Odpowiedzialnością System fragmentów lucyferazy z uniwersalnymi adapterami przeciwciał i białek do homogenicznej detekcji substancji w próbce lub testów interakcji molekularnych

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7879540B1 (en) * 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US20080233589A1 (en) * 2005-09-01 2008-09-25 Washington University In St. Louis Complementation Systems Utilizing Complexes of Heteroproteins

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12529699B2 (en) 2020-04-17 2026-01-20 Regeneron Pharmaceuticals, Inc Detection assays for coronavirus neutralizing antibodies

Also Published As

Publication number Publication date
JP2009159889A (ja) 2009-07-23
WO2009087967A1 (en) 2009-07-16

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