JP5230196B2 - 核酸のより効率的な単離のための器具および方法 - Google Patents

核酸のより効率的な単離のための器具および方法 Download PDF

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Publication number
JP5230196B2
JP5230196B2 JP2007520771A JP2007520771A JP5230196B2 JP 5230196 B2 JP5230196 B2 JP 5230196B2 JP 2007520771 A JP2007520771 A JP 2007520771A JP 2007520771 A JP2007520771 A JP 2007520771A JP 5230196 B2 JP5230196 B2 JP 5230196B2
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Prior art keywords
column
membrane
nucleic acids
buffer
binding
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JP2008506932A (ja
JP2008506932A5 (enExample
Inventor
トルシュテン ジンガー
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キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
    • B01D15/125—Pre-filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28033—Membrane, sheet, cloth, pad, lamellar or mat
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B01J20/28085—Pore diameter being more than 50 nm, i.e. macropores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/86—Sorbents applied to inner surfaces of columns or capillaries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/527—Physical parameters structural properties sorbent material in form of a membrane
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/177692—Oxides of nitrogen
    • Y10T436/178459—Only nitrogen dioxide

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Nanotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2007520771A 2004-07-15 2005-07-15 核酸のより効率的な単離のための器具および方法 Expired - Lifetime JP5230196B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102004034474.4 2004-07-15
DE102004034474A DE102004034474A1 (de) 2004-07-15 2004-07-15 Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren
PCT/EP2005/007726 WO2006008085A1 (de) 2004-07-15 2005-07-15 Vorrichtung und verfahren zur effizienteren isolierung von nukleinsäuren

Publications (3)

Publication Number Publication Date
JP2008506932A JP2008506932A (ja) 2008-03-06
JP2008506932A5 JP2008506932A5 (enExample) 2010-07-01
JP5230196B2 true JP5230196B2 (ja) 2013-07-10

Family

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JP2007520771A Expired - Lifetime JP5230196B2 (ja) 2004-07-15 2005-07-15 核酸のより効率的な単離のための器具および方法

Country Status (5)

Country Link
US (1) US8685742B2 (enExample)
EP (1) EP1771242B1 (enExample)
JP (1) JP5230196B2 (enExample)
DE (1) DE102004034474A1 (enExample)
WO (1) WO2006008085A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8034562B2 (en) * 2003-05-28 2011-10-11 Qiagen Gmbh Method for obtaining plasmid-DNA by means of an aqueous biphasic system
DE102004034474A1 (de) 2004-07-15 2006-02-16 Qiagen Gmbh Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren
DE602008001350D1 (de) 2007-10-31 2010-07-08 Roche Diagnostics Gmbh Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
US10883100B2 (en) * 2011-03-29 2021-01-05 Phynexus, Inc Devices and methods for plasmid purification
DE102014216016A1 (de) 2014-08-13 2016-02-18 Axagarius Gmbh & Co. Kg Vorrichtung zur Aufreinigung von Nukleinsäuren
RU2729113C2 (ru) 2014-11-21 2020-08-04 Оккам Байолэбс, Инк. Система и способ сбора образца нуклеиновой кислоты
WO2023056558A1 (en) * 2021-10-06 2023-04-13 Fredsense Technologies Corp. Methods and kits for isolating nucleic acids

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US4647380A (en) * 1982-09-22 1987-03-03 Dasgupta Purnendu K Annular dual membrane permselective device and method
JP2816739B2 (ja) * 1989-03-23 1998-10-27 旭光学工業株式会社 ウイルス及び細胞の分離器
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
EP0461477A1 (en) 1990-05-31 1991-12-18 Shimadzu Corporation Method of bacterial examination and apparatus therefor
JPH0436198A (ja) * 1990-05-31 1992-02-06 Shimadzu Corp 食中毒菌の検査方法
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
US5503816A (en) * 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
US5578459A (en) * 1993-11-24 1996-11-26 Abbott Laboratories Method and apparatus for collecting a cell sample from a liquid specimen
DE19512369A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh Vorrichtung zur Isolierung von Nukleinsäuren
EP1023460A4 (en) * 1997-06-25 2003-09-10 Promega Corp METHOD FOR ISOLATING RNA
DE19734135A1 (de) * 1997-08-07 1999-02-11 Boehringer Mannheim Gmbh System zum Zurverfügungstellen biologischer Materialien
JP2000162208A (ja) * 1998-11-27 2000-06-16 Fuji Photo Film Co Ltd ガラス繊維濾紙の製造方法
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
JP2002209580A (ja) 2001-01-15 2002-07-30 Jsr Corp 核酸の分離方法および球状担体
JP2002345465A (ja) * 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 核酸精製ユニット及び核酸精製方法
US6818762B2 (en) * 2001-05-25 2004-11-16 Maine Molecular Quality Controls, Inc. Compositions and methods relating to nucleic acid reference standards
JP2002191351A (ja) * 2001-10-19 2002-07-09 Hitachi Ltd 核酸の精製用装置および核酸捕捉用チップ
EP1321176A1 (en) 2001-12-18 2003-06-25 Boehringer Ingelheim International GmbH Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
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US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
DE102004034474A1 (de) 2004-07-15 2006-02-16 Qiagen Gmbh Vorrichtung und Verfahren zur effizienteren Isolierung von Nukleinsäuren

Also Published As

Publication number Publication date
WO2006008085A1 (de) 2006-01-26
JP2008506932A (ja) 2008-03-06
US8685742B2 (en) 2014-04-01
US20080039619A1 (en) 2008-02-14
DE102004034474A1 (de) 2006-02-16
EP1771242B1 (de) 2020-04-22
EP1771242A1 (de) 2007-04-11

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