JP5190213B2 - 厚味付与機能を有する新規ペプチド、並びにこれらを使用する食品の厚味付与方法 - Google Patents
厚味付与機能を有する新規ペプチド、並びにこれらを使用する食品の厚味付与方法 Download PDFInfo
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Description
(1)酵母から生成でき、厚味付与機能を有するペプチド。
(2)下記配列式(I)(II)(III)(IV)(V)(VI)(VII)(VIII)(IX)(X)で表される配列のいずれか1つまたは2つ以上からなる厚味付与機能を有するペプチド。
配列式(I)Asp−Trp−Arg−Gly−Gly−Arg
配列式(II)Asp−Trp−Arg−Gly−Gly−Arg−Thr
配列式(III)Phe−Lys−Tyr
配列式(IV)Val−Leu−Gly−Tyr
配列式(V)Leu−Gly−Gln−Trp
配列式(VI)Ala−Ile−Gly−Glu−Trp
配列式(VII)Ile−Thr−Trp−Glu
配列式(VIII)Val−Trp−Glu−Tyr
配列式(IX)Phe−Asp−Trp
配列式(X)Phe−Glu−Trp
(3)(2)記載の配列式中トリプトファン残基を含有することを特徴とする、下記配列式(a)(b)(c)(d)(e)(f)(g)(h)で表される配列から、いずれか1つまたは2つ以上を選択してなる(2)記載の厚味付与機能を有するペプチド。
配列式(a)Asp−Trp−Arg−Gly−Gly−Arg
配列式(b)Asp−Trp−Arg−Gly−Gly−Arg−Thr
配列式(c)Leu−Gly−Gln−Trp
配列式(d)Ala−Ile−Gly−Glu−Trp
配列式(e)Ile−Thr−Trp−Glu
配列式(f)Val−Trp−Glu−Tyr
配列式(g)Phe−Asp−Trp
配列式(h)Phe−Glu−Trp
(4)(2)又は(3)記載のペプチドを含有することを特徴とする酵母蛋白質分解物または酵母エキス。
(5)(2)又は(3)記載のペプチド又は(4)記載の酵母蛋白質分解物または酵母エキスを含有する調味料または食品。
(6)食用素材を酵素処理又は自己消化又は抽出し、生成することを特徴とする、(2)又は(3)記載のペプチドの製造方法。
(7)化学的合成法により合成することを特徴とする(2)又は(3)記載のペプチドの製造方法。
配列式(I)Asp−Trp−Arg−Gly−Gly−Arg
配列式(II)Asp−Trp−Arg−Gly−Gly−Arg−Thr
配列式(III)Phe−Lys−Tyr
配列式(IV)Val−Leu−Gly−Tyr
配列式(V)Leu−Gly−Gln−Trp
配列式(VI)Ala−Ile−Gly−Glu−Trp
配列式(VII)Ile−Thr−Trp−Glu
配列式(VIII)Val−Trp−Glu−Tyr
配列式(IX)Phe−Asp−Trp
配列式(X)Phe−Glu−Trp
配列式(I) 10
配列式(II) 17
配列式(III) 17
配列式(IV) 12
配列式(V) 21
配列式(VI) 6
配列式(VII) 5
配列式(VIII) 6
配列式(IX) 3
配列式(X) 2
厚味付与ペプチドの単離精製および合成を実施例1に示す。
酵母エキスはイーストエキス21TF(日本たばこ産業(株))を使用した。この酵母エキスを固形分が10%相当になる様に加水した上で遠心分離を実施し、上清を回収後、0.45μmフィルトレーションにより分画前サンプル(以下21TF)とした。本サンプルをオクタデシルシリカ(ODS)樹脂にて充填されたカラムに通液した。素通りした液を回収し、続いて蒸留水を通水し画分を得た(以下水画分)。続いて5%エタノールを通液し溶出画分(以下5%画分)を得た。同様に10%エタノールを用いて溶出画分(以下10%画分)、30%エタノール、60%エタノールおよび100%エタノールにて溶出画分(以下100%画分)を得た。
実施例1で示した本発明のペプチド混合物の、添加量による厚味付与効果への影響を調べるために以下の実験をした。
まず始めに、訓練されたパネラー9名にイーストエキス21TF(日本たばこ産業(株))を試飲させ、厚味の認識をさせた。
官能試験はそれぞれの濃度におけるイーストエキスと厚味の強度が同じものを5点とし、大きく劣るものを1点とし、1〜5点のスコアを標記させ、平均点で表した。試験は前記の訓練されたパネル9人によって行った。結果を表3に示す。
実施例2で示した、好ましい呈味効果を得る為の該ペプチドの添加量の範囲において、該10種ペプチドの混合比を検討した。ここではイーストエキス21TFの1.0%液を基準とし、その時のペプチド溶液のペプチド総量は実施例2と同一とした上で、その混合比のみを変更した検討を行った。混合比の変更は該10種ペプチドそれぞれについて、実施例2における比を基準とした3通りを調製し、官能試験1〜10を実施した。官能試験それぞれは実施例2における比での厚味の強度における点数を3点とし、大きく勝るものを5点、大きく劣るものを1点とし、1〜5点のスコアを標記させ、平均点で表した。試験は前記の訓練されたパネル9人によって行った。結果を表4〜13に示す。
該ペプチドによる他エキスへの厚味付与効果を、カツオ、野菜、ポークおよびチキンエキスにおいても検討した。試験は訓練されたパネル8人によって行った。ここで添加するペプチドは実施例2に準拠し、固形分濃度0.5%のイーストエキス21TFに含有される量(0.000175%)および相対比を有するペプチド混合物を用いた。カツオエキス、ポークエキスおよびチキンエキスは次に示す自社製品を使用した。カツオエキス「水出しかつおだしP」、ポークエキス「ポークエキスHP」、チキンエキス「チキンエキスS」。また野菜エキスは井村屋製菓(株)製品「ヤサイブイヨンIM」を使用した。結果を表14に示す。
実施例2に準拠する形で対照液のかわりに新たに和風ダシを作成し、当該10種ペプチド混合物の添加による和風ダシへの厚味の付与効果を確認した。添加量は21TFの固形分1.0%溶液に含まれる量(0.00035%)となるように添加した。和風ダシの処方を表15に示す。
Claims (5)
- 下記配列式(I)(II)(III)(IV)(V)(VI)(VII)(VIII)で表される配列のいずれか1つまたは2つ以上からなる酵母由来の厚味付与機能を有するペプチド。
配列式(I)Asp−Trp−Arg−Gly−Gly−Arg
配列式(II)Asp−Trp−Arg−Gly−Gly−Arg−Thr
配列式(III)Val−Leu−Gly−Tyr
配列式(IV)Leu−Gly−Gln−Trp
配列式(V)Ala−Ile−Gly−Glu−Trp
配列式(VI)Ile−Thr−Trp−Glu
配列式(VII)Val−Trp−Glu−Tyr
配列式(VIII)Phe−Asp−Trp - 請求項1記載の配列式中トリプトファン残基を含有することを特徴とする、下記配列式(a)(b)(c)(d)(e)(f)(g)で表される配列から、いずれか1つまたは2つ以上を選択してなる請求項1記載の酵母由来の厚味付与機能を有するペプチド。
配列式(a)Asp−Trp−Arg−Gly−Gly−Arg
配列式(b)Asp−Trp−Arg−Gly−Gly−Arg−Thr
配列式(c)Leu−Gly−Gln−Trp
配列式(d)Ala−Ile−Gly−Glu−Trp
配列式(e)Ile−Thr−Trp−Glu
配列式(f)Val−Trp−Glu−Tyr
配列式(g)Phe−Asp−Trp - 請求項1又は2記載のペプチドを添加することを特徴とする調味料又は食品への厚味付与方法。
- 酵母を酵素処理又は自己消化若しくは抽出することを特徴とする請求項1又は2記載のペプチドの製造方法。
- 化学的合成法により合成することを特徴とする請求項1又は2記載のペプチドの製造方法。
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