JP5066670B2 - Anti-obesity agent and edible composition - Google Patents

Description

The present invention relates to an anti-obesity agent comprising at least actinidine as an active ingredient and an edible composition comprising the same.

In recent years, obesity has become an important health concern in Japan. The reason why obesity is attracting attention is due to the fact that obese people frequently develop so-called lifestyle-related diseases such as heart disease, arteriosclerosis, hypertension, and diabetes, and have a high mortality rate. Obesity is known to be a major factor in inducing diabetes, hyperlipidemia, hypertension, fatty liver, and arteriosclerosis. The major causes of death among Japanese are cancer, heart disease, and stroke. However, considering that heart disease and stroke are the main causes of arteriosclerosis, it is understood that there are very many causes of death from arteriosclerotic diseases. it can.

The state of obesity is generally the result of abnormal accumulation of energy sources such as carbohydrates and lipids that are not consumed by various activities such as exercise among the ingested energy, A state in which the body weight has increased beyond the limits of the skeletal system or physiological function. And it becomes clear that the distribution state of the accumulated fat in which the energy source is accumulated as fat in the living body is important in relation to the above-mentioned various diseases. In particular, internal fat type obesity (also called visceral obesity or abdominal obesity) ) Is a combination of two or more of hyperglycemia, hypertension and hyperlipidemia is called metabolic syndrome (metabolic syndrome), and even if each symptom is mild, it is synergistic by overlapping multiple symptoms As the incidence of arteriosclerotic diseases increases, the prevention and treatment are attracting attention as a high-risk group.

Conventionally, the degree of deviation from the standard weight, the BMI (body mass index), the body fat percentage, and the like have been used as indices for determining the obesity state, but these can determine the obesity state only visually or indirectly. For this reason, a method of measuring the distribution state of accumulated fat using a CT (Computed Tomography) apparatus, obtaining the accumulated fat area from the image, and determining obesity based on this area has been developed and is becoming popular.

By the way, in order to prevent obesity, many attempts have been made to prevent obesity through treatment and prevention in the medical field as well as through daily meals and various foods and drinks. For example, L-carnitine or a livestock peptide containing the same as an edible material that aims to regulate metabolism of ingested lipids, promote combustion of body fat, or suppress accumulation (Patent Documents 1, 2, and 3) , Algal extracts (Patent Document 4), carotenoids (Patent Document 5), anthocyanidins (Patent Document 6), green tea catechins (Patent Document 7), porphyran modified low molecular weight compounds (Patent Document 8), etc. Yes.

However, most of them have proposed the possibility of application to humans based on the test data of cultured cells or experimental animals. There were a few actual cases. Therefore, there has been a demand for an edible material that has a clear obesity-preventing effect or body fat accumulation-suppressing effect even in practical use.

JP-A-7-196485 JP-A-10-66515 JP 2000-256200 A JP 2000-72642 A JP 2003-95930 A JP 2002-153240 A JP 2006-131512 A JP 2006-104100 A

In view of such circumstances, an object of the present invention is to provide an anti-obesity agent that is excellent in safety, can be easily incorporated into a diet, and is effective even in humans, and an edible composition containing the same. .

In order to solve the above-mentioned problems, the present inventors have made extensive studies on obesity symptoms and materials for improving them, and as a result, actinidine unexpectedly significantly improves the accumulation of visceral fat and / or subcutaneous fat. The present invention has been completed by finding that it is possible, effective in improving obesity symptoms, and that it can be effectively used in edible compositions such as foods and drinks.

That is, according to the present invention, there is provided an anti-obesity agent comprising actinidine as an active ingredient.

The anti-obesity agent of the present invention is more preferably one containing actinidine and ascorbic acid as active ingredients, and more preferably one containing actinidine, ascorbic acid and dietary fiber as active ingredients.

In the anti-obesity agent of the present invention, the above-mentioned active ingredients, that is, actinidin, actinidine and ascorbic acid, and actinidin and ascorbic acid and dietary fiber are preferably obtained from kiwifruit, and desirable aspects of kiwifruit are Kiwi fruit pulp, fruit juice, or an extract or purified product thereof.

The active ingredient of the anti-obesity agent of the present invention is preferably in a form in which at least actinidine is coated with or adsorbed to a hydrophilic substance.

The obesity prevention in the anti-obesity agent of the present invention is preferably due to a reduction in body fat. The reduction of body fat is preferably suppression of visceral fat accumulation and / or suppression of subcutaneous fat accumulation, and more preferably by suppression of visceral fat accumulation.

According to the present invention, there is also provided an edible composition comprising any of the anti-obesity agents, and the edible composition is preferably a food or drink. Furthermore, the edible composition contains any of the anti-obesity agents, and is said to prevent obesity, reduce body fat, suppress visceral fat accumulation, or subcutaneously. At least one of the indications that fat accumulation is suppressed is given.

The anti-obesity agent of the present invention is excellent in safety because it uses substances that have been conventionally used for food and obtained from raw materials with abundant dietary experience, and when ingested by humans, body weight, visceral fat and subcutaneous fat Can significantly reduce obesity symptoms. Further, the anti-obesity agent of the present invention can be taken alone orally and used as a dietary supplement for improving obesity, and can also be taken as a processed food for a normal meal menu. It can also be used in the form of animals other than humans, for example, livestock or pet food or oral intake. Furthermore, the anti-obesity agent of the present invention can also be expected to have an effect of preventing the development of obesity symptoms. Therefore, the anti-obesity agent of the present invention and the edible composition containing the same can be applied as an oral intake corresponding to lifestyle-related diseases and metabolic syndrome.

The present invention is described in detail below. First, the antiobesity agent of the present invention is characterized by containing actinidine as an active ingredient.

Actinidine is a kind of proteolytic enzyme having a molecular weight of about 24 kDa, and is classified as a cysteine protease (EC 3.4.22.14). It is contained in the fruits of plants belonging to the genus Actinidia such as kiwifruit, matatabi, and sarunasi, and is used as a food additive. It is known for its actions such as gelatin gelatinization suppression, meat softening and decomposition, and tongue coating removal. It has been. In the anti-obesity agent of the present invention, such actinidine is contained alone or together with the specific raw material components described below as an active ingredient.

The mode of the actinidine according to the present invention may be one or more selected from the group consisting of fruits, fruit juices, dried products, extracts, and purified products of the genus Matabi, but handleability From the viewpoint of product stability and production cost, the extract is preferable. The extract is obtained by appropriately chopping or squeezing the whole fruit of the genus Matabia plant or the flesh excluding the outer skin and / or the fruit core part to obtain fruit juice, which is equi-weight to about 50 times weight of water or After adding water-containing ethanol and mixing well, the insoluble matter can be removed by filtration or centrifugation, the extract is collected, and the extraction solvent can be removed by drying under reduced pressure, freeze drying, or the like. Here, Kiwifruit is preferable as the genus Matabi, because it is easily available. In order to prevent the activity of actinidin from decreasing, it is desirable that the water-containing ethanol has an ethanol concentration of about 10% by weight or less. Each treatment should be operated at about 60 ° C. or less, more preferably 30 ° C. or less. The origin of the actinidine according to the present invention is not limited to the plant of the genus Matabi, and actinidine is obtained by purifying the extract to a higher purity by known means such as fractionation, fractionation, column chromatography and the like. But you can. In view of the object of the present invention, the actinidine according to the present invention and its inclusions contain a hydrophobic substance such as hardened fats and oils, wax, solid paraffin, cellulose, etc. in order to suppress a decrease in actinidin activity in the stomach during oral ingestion. It is desirable to coat with a known method using a hydrophilic substance such as a cellulose derivative or modified starch, and process it into a granular or powdered product.

A more desirable aspect of the anti-obesity agent of the present invention is characterized by containing actinidine and ascorbic acid as active ingredients.

In this embodiment, the aforementioned actinidine can be used. Ascorbic acid includes ascorbic acid, its sodium, potassium, calcium and magnesium salts, esters with fatty acids such as palmitic acid and oleic acid, iron ascorbate, ascorbic acid diphosphate, etc. Well-known ones can be used. The ratio of ascorbic acid based on actinidine (weight basis; the same applies hereinafter) is actinidine: ascorbic acid = 1: 0.01-10, more preferably 1: 0.05-5, and even more desirably 1: 0. .1-3. In the present invention, by using actinidine and ascorbic acid in combination, a stronger anti-obesity effect can be expressed as compared with the case where only actinidine is used as the active ingredient. If the ratio between the two is less than the lower limit of the above range, the desired effect due to the combined use is hardly exhibited, and even if the combined ratio exceeds the upper limit of the above range, no further desired effect can be expected.

A further desirable aspect of the anti-obesity agent of the present invention is characterized by comprising actinidine, ascorbic acid and dietary fiber as active ingredients.

In this embodiment, the aforementioned actinidine and ascorbic acid can be used. Dietary fiber is defined in Japan as “the total of indigestible ingredients in foods that are not digested by human digestive enzymes” (“Five Japanese Food Standard Ingredients Table” (edited by the Agency for Science and Technology Resources)), almost all As a physiological action, it is known to improve colon function (improvement of bowel movement and colon environment), improve blood sugar level, improve blood cholesterol, and the like. Generally classified into insoluble dietary fiber and water-soluble dietary fiber, the former mainly contains polysaccharides such as cellulose, hemicellulose, and pectin, lignin, chitin, etc., which are components of plant cell walls, and the latter is plant cytoplasm Pectin, seaweed polysaccharides (alginic acid, laminarin, fucoidan, etc.), glucomannan, guar gum, gum arabic, acacia fiber, galactomannan, microbial polysaccharides (xanthan gum, curdlan, pullulan, etc.), chemical processed products (methylcellulose, Cellulose derivatives such as hydroxypropylcellulose, modified starch, indigestible dextrin, polydextrose and the like), oligosaccharides and the like.

In the anti-obesity agent of the present invention, the dietary fiber used in combination with actinidine is not limited to the above-mentioned examples, and known ones can be used. The ratio of actinidine to dietary fiber is actinidine: dietary fiber = 1: 0.1 to 100, more preferably 1: 1 to 50, and even more desirably 1: 2 to 30. The ratio of actinidine and ascorbic acid may be the same as described above. In the present invention, by using actinidine, ascorbic acid and dietary fiber in combination, the effect of preventing obesity is even more prominent than when only actinidine is used as an active ingredient or when actinidine and ascorbic acid are used as active ingredients. Can be made. If the ratio of these three components is below the lower limit of the range, the desired effect due to the combined use tends to be reduced, and even if the upper limit of the range is exceeded, a further desired effect cannot be expected.

The obesity-preventing agent of the present invention may be used in combination with known obesity-improving action, weight-reducing action, dieting action, dietary lipid absorption inhibiting action, body fat burning promoting action, and the like, and materials containing the same. Examples of these are green tea catechin, soybean oil unsaponifiable, soybean isoflavone, α-lipoic acid, carnitine, coenzyme Q10, phaseolamin, caffeic acid, dihydroxychalcones, branched amino acids (valine, leucine, isoleucine), anthocyanidins, levan, Betaine, ellagitannin, low molecular weight chitosan, rosmarinic acid and its esters, conjugated polyenoic acid, curcumin, resveratrol, forskolin, bergenin, sclerotioline, diacylglycerol, low molecular compounds of porphyran, chlorogenic acid, carotenoid (lycopene, lutein, Zeaxanthin, etc.), gymnemic acid, hydroxycitric acid, astilbine, heat-modified gum arabic, hesperidin, low molecular weight pectin with a molecular weight of 50,000 to 150,000, beer yeast production mannan, Gin, oleanol, oleanolic acid, eugenol, theaflavin, soybean saponin, phenyl-OD-glucose, 3,4,6-trimethoxy-OD-glucose, itaconic acid and its salt, hydroxyproline and its N-acyl derivative , Α-starch and sucrose fatty acid ester conjugate, procyanidin, cyclic and chain polylactic acid having a polymerization degree of 3 to 19, α-methyl-D-xyloside, xylooligosaccharide, L-arabinose, indigestible dextrin, baronea Examples include acids and glycosides thereof, capsaicin, polyphenol, salacinol, ganoderic acid, soy peptide, whey peptide, beef peptide, bran, rice bran, psyllium seed coat, and isolated soy protein.

Moreover, the dried material of each site | part of the following plant, seaweed, moss, etc., those water, or a lower alcohol extract can be illustrated. That is, guava leaves, gymnema sylvestre leaves, Garcinia cambodia peel, perilla leaves or seeds, evening primrose seeds, quilla bark, eucalyptus leaves, peony flowers, totu leaves, black rice, peanut astringent bark, oolong tea leaves, banana peels, itadori stems, grape seeds Pericarp, cassis fruit, cypress root, yellow leaves, olive leaves, kehi bark, asunaro whole grass, chestnut astringent bark, kumaza leaf, birch bark, amla fruit, mulberry leaves, chickpeas, red pepper root, coleus forskori root, kawara ketsumei leaf or saya, Turmeric Root, Seaweed, Clove Mushroom, Oregano Whole Grass, Red Megashi Bark, Vulgaris Bark, Hypericum Whole Grass, Salacia Reticulata Root, Shrimp Leaf, Aloe Mesophyll, Ekokogi Root, Ganoderma, Maitake, Enokitake, Birch Rice germ, kale And the like. In addition, the antiobesity agent of this invention is not restrict | limited by these illustrations.

The anti-obesity agent of the present invention comprises about 0.01% to 100% by weight, more preferably about 0.1% to about 80% by weight of actinidine as an essential raw material component with respect to the total amount of the agent, Ascorbic acid and dietary fiber are appropriately selected within the range of the above-mentioned ratios to actinidine, and together with these, known ingredients and their containing materials having the above-mentioned action related to obesity prevention, as needed for oral intake Commonly used known excipients, binders, disintegrants, lubricants, wetting agents, fluidizing agents, preservatives, surfactants, stabilizers, diluents, solubilizers, isotonic agents, bactericides In addition, additives such as preservatives, flavoring agents, flavoring agents, coloring agents, and fragrances can be appropriately used in combination, and can be prepared by a conventional method. The form of the agent can be an oral preparation such as a granule, a tablet, a capsule or a liquid, or a composition in a form blended with a general processed food or drink.

The usefulness of the anti-obesity agent of the present invention is that it exhibits remarkable effects not only in terms of weight reduction but also in reducing body fat, more specifically in suppressing visceral fat accumulation and subcutaneous fat accumulation. Therefore, the anti-obesity agent of the present invention is suitably applied for reducing body fat, further for suppressing visceral fat accumulation and / or for suppressing subcutaneous fat accumulation.

In the anti-obesity agent of the present invention, it is preferable to use an active ingredient in a form in which at least actinidine is coated with a hydrophilic substance or adsorbed or mixed with a hydrophilic substance. By forming such a coating, adsorbate, or mixture, it is possible to suppress a decrease in activity due to actinidin oxidation and the like to enhance the stability, and it is easy to blend in an aqueous composition such as a beverage. Here, the hydrophilic substance is a substance having an affinity for an aqueous substance, and preferably has a coating film-forming ability. Specific examples include polysaccharides (xanthan gum, guar gum, tamarind seed gum, psyllium seed gum). Etc.), starch and modified starch, yeast cell wall components, glucan, mannan, shellac, sodium alginate, gelatin, carrageenan, pullulan, various cellulose derivatives such as carboxymethylcellulose, soy protein, whey protein, zein, etc. . More preferred are one or more selected from the group consisting of polysaccharides, starch, yeast cell wall components, shellac, gelatin, soybean protein, zein and mannan, and more preferred are yeast cell wall components and shellac. In order to coat such a hydrophilic substance, the hydrophilic substance is appropriately made into a liquid substance dissolved in water, ethanol or other solvent, and this is adhered to an actinidine or an actinidine-containing substance and dried to form a hydrophilic substance. A coating film can be formed. It is also a desirable aspect to form an inclusion using cyclodextrin (α type, β type, γ type, δ type, etc.).

The anti-obesity agent of the present invention comprises actinidine, more preferably actinidin and ascorbic acid, more preferably actinidin, ascorbic acid and dietary fiber as essential components, and itself contains food and drink, pharmaceuticals, feeds, It can be made into various products in the cosmetics and other industrial fields, and can also be used in an embodiment in which it is used as a part of blended raw materials of these various products. It can be applied to oral uses such as edible compositions, and is particularly suitable for food and drink use. This example will be described below, but the present invention is not limited by these.

Specific examples of food and drink include beverages such as vegetable juice, fruit juice drink, soft drink, tea, soup, jelly, pudding, yogurt, cake premix product, confectionery, sprinkle, miso, soy sauce, sauce, dressing, mayonnaise, Vegetable cream, miso, seasonings such as grilled meat sauce and noodle soup, noodles, udon, soba noodles, spaghetti, ham and sausage, etc. , Powders such as spreads and cheese, solid or liquid dairy products, margarine, bread, cakes, cookies, chocolates, candy, gummi, gums, and other general processed foods, powders, granules, pills , Tablets, soft capsules, hard capsules, pasty or liquid dietary supplements, food for specified health use, machine Sex food, mention may be made of health food products, the concentrated liquid diet and dysphagia for food diet and the like. In the edible composition of the present invention, the effect that the edible composition is for obesity prevention, the effect of reducing body fat, the effect of suppressing visceral fat accumulation, or Further, it is possible to adopt an aspect in which at least one indication is given to the effect of suppressing subcutaneous fat accumulation.

In order to produce these foods and drinks, the known raw materials and the anti-obesity agent of the present invention may be used, or a part of the known raw materials may be replaced with the anti-obesity agent of the present invention and produced by conventional methods. For example, the obesity-preventing agent of the present invention may be added to excipients such as glucose (dextrose), dextrin, lactose, starch or processed product thereof, cellulose powder, vitamins, minerals, fats and oils of animals and plants and seafood, proteins as necessary. (Proteins derived from plants and animals, hydrolysates thereof, etc.), carbohydrates, pigments, fragrances, antioxidants, surfactants, other edible additives, edible materials such as powders and extracts containing various nutritional functional ingredients Mixed with it and processed into powder, granules, pellets, tablets, etc., processed into general foods in the above examples by conventional methods, and mixed liquids such as gelatin, sodium alginate, carboxymethylcellulose, etc. It is suitable to use as a dietary supplement or health food by coating with an agent and forming into a capsule, or processing into a beverage (drink) form. In particular, tablets, capsules and drinks are desirable.

The ratio of the anti-obesity agent of the present invention to be blended in such food and drink is different in the form of the food and drink, the content of actinidine, ascorbic acid and dietary fiber in the anti-obesity agent of the present invention, and the types and ingredients of other ingredients. However, actinidin, its so that the actinidine content in the food and drink is about 0.01 wt% to about 90 wt%, more preferably about 0.1 wt% to about 50 wt% In the case of using inclusions and using dietary fiber in combination, actinidin: dietary fiber = 1: 0.1 to 100, more preferably 1: 1 to 50, and even more preferably 1: 2 to 30 When dietary fiber and ascorbic acid are used in combination, the ratio of dietary fiber to actinidine is the same as described above, and actinidine: ascorbic acid = 1: 0.01-10, more preferably 1: 0.0. ~ 5, more desirably, using ascorbic acid at a ratio of 1: 0.1-3, and appropriately combining these with other raw materials for food and beverage production to design a recipe and preparing the desired food and beverage according to conventional methods do it. In foods and beverages with an actinidine content of less than about 0.01% by weight, a large amount of the foods and beverages must be consumed in order to expect the desired effect of actinidin, while about 90% by weight of the foods and beverages of the present invention. The maximum practical amount. In the case of humans (adults), the food and drink of the present invention can be applied to any method with a daily actinidine intake standard of about 10 mg to about 1000 mg, preferably about 20 mg to about 500 mg, more preferably about 50 mg to about 200 mg. For example, it can be taken into the body by methods such as oral ingestion and tube administration.

Next, although an Example is given and this invention is demonstrated in detail, this invention is not limited at all by this. In each example,%, part and ratio are all on a weight basis.

Production Example 1 (Purified product of actinidine)
Two commercially available New Zealand kiwifruits (Hayward seeds) were squeezed by removing the fruit skin of 210 g of fruit skin, and the juice was obtained by centrifugation (4 ° C., 10,000 rpm, 10 minutes) to obtain the supernatant. Sorted. After adjusting the pH to 6.0 by adding a 5 molar aqueous sodium hydroxide solution to this supernatant, the method of Blocklehurst et al. (Blocklehurst K, et.al., The Biochemical Journal, Vol. 197, pages 739 to 746, 1981), an actinidine-containing fraction was obtained by an ammonium sulfate precipitation treatment. Subsequently, this fraction was purified by covalent chromatography using a thiopropyl-Sepharose 6B (GE Healthcare Bioscience) column, and a purified actinidine-containing liquid containing actinidin as almost a single component was collected, freeze-dried 49 mg of purified actinidine (sample 1) was prepared. This was almost pure actinidine (molecular weight: a single band of about 24 kDa) as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method.

Production Example 2 (mixture of actinidine and ascorbic acid)
Sample 1 and ascorbic acid (manufactured by DSM Nutrition Japan Co., Ltd., fine powder) were mixed to prepare a mixed composition of sample 1: ascorbic acid = 1: 2 (sample 2).

Production Example 3 (mixture of actinidine, ascorbic acid and dietary fiber)
Sample 1 and ascorbic acid (DSM Nutrition Japan Co., Ltd., fine powder) were mixed with indigestible dextrin (Matsuya Chemical Industry Co., Ltd., Fiber Sol 2 (registered trademark)) as a dietary fiber. A mixed composition (sample 3) of 1: ascorbic acid: dietary fiber = 1: 0.5: 15 was prepared.

Production Example 4 (Actinidin-containing extract)
Squeeze 980 g of commercially available New Zealand kiwifruit (Hayward seed) 980 g to obtain squeezed juice, add 5 times the amount of water to this squeeze, mix, filter insolubles, freeze A powdery actinidine-containing extract (sample 4) was prepared by drying and grinding. This included 3.7% actinidin, 0.9% ascorbic acid and 9.4% water soluble dietary fiber. The actinidine content was determined by the method described in the literature by Nishiyama et al. (Ichiro Nishiyama et al., Japanese Journal of Food Science and Technology, Vol. 49, pages 401 to 408, 2002). That is, an electrophoretic image obtained by SDS-PAGE using 12% polyacrylamide gel with sample 1 as a standard substance and bovine serum albumin (Sigma Co., Reagent) as an internal standard, gel imaging device and image analysis system (Ato Co., Ltd., AE-6940V-FX densitograph) was quantitatively analyzed.

Production Example 5 (Actinidin coating)
After mixing 30 g of Sample 1 separately prepared by the method described in Production Example 1 and 100 mL of a dispersion containing 7.5% yeast cell wall (trade name: Yeast Wrap (registered trademark) manufactured by Kirin Brewery Co., Ltd.) Then, it was freeze-dried to prepare a coated product (sample 5) of actinidine purified product.

Production Example 6 (Coating of a mixture of actinidine and ascorbic acid)
After mixing 200 g of Sample 2 separately prepared by the method described in Production Example 2 and 900 mL of a dispersion containing 7.6% yeast cell wall (trade name: Yeast Wrap (registered trademark), manufactured by Kirin Brewery Co., Ltd.) Then, it was freeze-dried to prepare a coating (sample 6) of a mixture of purified actinidine and ascorbic acid.

Production Example 7 (Adsorbed material containing actinidine)
50 mL of an aqueous solution containing 10 g of Sample 4 separately prepared by the method described in Production Example 4 and 4.5 g of microcrystalline cellulose (manufactured by Kenko Tsusho Co., Ltd.) are mixed and then dried at 10 ° C. to contain actinidin A product adsorbate (Sample 7) was prepared.

Test example 1 (anti-obesity effect)
The effect of the anti-obesity agent of the present invention on the recovery and improvement of obesity symptoms was examined by the following method. That is, gelatin hard capsules each filled with 155 mg of Samples 1 to 7 according to the present invention were prepared. As a comparative sample, a sample obtained by heat-treating sample 1 at 80 ° C. for 30 minutes was similarly filled in a hard capsule (sample 8). Under the guidance of a doctor, each sample was served twice a day for volunteers including those with a BMI of 25 or more (5 men and 5 women in total, age: 20 to 77 years old) for each sample. Before 2 weeks 3 capsules / dose, 5 capsules / day was taken for 3 weeks. During this test period, at the start of ingestion and after 3 weeks, height and weight measurement, BMI calculation, and abdominal tomography were performed using an X-ray CT apparatus, and visceral fat area and subcutaneous fat area were measured. Some results are shown in Tables 1 and 2. The numerical values in each table are average values. BMI = weight (kg) ÷ height (m) ÷ height (m).

From the data in Table 1 and Table 2, when samples 1 to 7 according to the present invention were ingested, body weight, BMI, visceral fat area, subcutaneous fat area and body fat after 3 weeks compared to the start of ingestion It became clear that the total area decreased. These reduction rates were as follows: body weight 1.3% to 2.7%, BMI 4.3% to 9.4%, visceral fat area 12.9% to 23.0%, subcutaneous fat area 11. The total body fat area was 13.0% to 18.7%. On the other hand, when the sample 9 (comparative sample) was ingested, the rate of decrease of the evaluation index was as follows: body weight 0.6%, BMI 1.5%, visceral fat area 6.7%, subcutaneous fat area Was 5.8%, and the total body fat area was 6.2%. From this, by taking actinidin, it is possible to reduce body weight and BMI, to reduce and suppress body fat accumulation, and particularly to reduce and inhibit visceral fat accumulation that has a large causal relationship with the above-mentioned various diseases. It has been confirmed that the anti-obesity effect is practical and useful in human subjects. This effect is remarkable when ascorbic acid is used in combination with actinidine (sample 2) or when ascorbic acid and dietary fiber are used together (sample 3). In the case of these coatings (sample 5 to sample 7) But it was the same. Furthermore, since all these samples are taken before every meal, it is possible to sufficiently expect a preventive effect as well as an effect of improving obesity symptoms.

(Example 1: Food and drink)
Actinidin purified product (sample 1), actinidine purified product and ascorbic acid mixture (sample 2), actinidine purified product and a mixture of ascorbic acid and dietary fiber (sample 3), actinidine-containing extract ( Sample 4), actinidine purified product coating (sample 5), actinidine purified product and ascorbic acid mixture coating (sample 6), and actinidine-containing extract adsorbate (sample 7) Using a filling machine, a gelatin-coated hard capsule preparation having an inner volume of 150 mg per grain was prepared by a conventional method. The other samples were processed in the same manner to prepare 7 types of gelatin-coated hard capsule formulations. These capsule preparations can be used as dietary supplements that can be taken orally.

(Example 2: Food and drink)
30 parts of actinidine-containing extract (sample 4) as an obesity preventing agent of the present invention, 0.5 part of senna stem extract (manufactured by BN Co., Ltd.), 105 parts of corn starch (manufactured by Nippon Corn Starch Co., Ltd.), triphosphate 50 parts of calcium (manufactured by Yoneyama Chemical Co., Ltd.) and 7 parts of riboflavin (manufactured by DSM Nutrition Japan Co., Ltd.) were charged into a mixer and stirred and mixed for 10 minutes. This mixture was subjected to a direct compression tableting machine to prepare uncoated tablets having a diameter of 7 mm, a height of 4 mm, and a weight of 150 mg / piece, and then a shellac film was formed by the coating machine to produce a tablet-shaped food.

(Example 6: Food and drink)
A homogeneous orange flavored beverage was prepared by adding 15 g of a mixture of a purified product of actinidine as an anti-obesity agent of the present invention and a coating of ascorbic acid (sample 6) to 1 L of commercially available orange juice and mixing well. Even when stored in the refrigerator for 3 weeks, the appearance and flavor were neither abnormal nor uncomfortable. This product is intended to prevent obesity, to reduce body fat, to suppress visceral fat accumulation, or to suppress subcutaneous fat accumulation. At least one indication can be given.

Since the anti-obesity agent comprising actinidin, actinidine and ascorbic acid, actinidine and ascorbic acid and dietary fiber as active ingredients of the present invention has an action of improving obesity symptoms by ingesting this, obesity symptoms It can be effectively used for foods and drinks, pharmaceuticals, quasi drugs, feeds, etc.

Claims (8)

An anti-obesity agent containing actinidine as an active ingredient (except for food and drink use) . An anti-obesity agent containing actinidine and ascorbic acid as active ingredients (excluding food and drink use) . An anti-obesity agent containing actinidin, ascorbic acid and dietary fiber as active ingredients (excluding food and drink use) .   The anti-obesity agent according to any one of claims 1 to 3, wherein the active ingredient is obtained from kiwifruit as a raw material.   The anti-obesity agent according to claim 4, wherein the kiwifruit is kiwifruit pulp, fruit juice, or an extract or purified product thereof.   The anti-obesity agent according to any one of claims 1 to 5, wherein at least actinidine among the active ingredients is coated with a hydrophilic substance or adsorbed to the hydrophilic substance.   The obesity prevention agent according to any one of claims 1 to 6, wherein obesity prevention is due to reduction of body fat.   The anti-obesity agent according to claim 7, wherein the reduction in body fat is suppression of visceral fat accumulation and / or suppression of subcutaneous fat accumulation.