JP2011032237A - Composition containing low-molecule polysaccharide which has anti-obesity action, and composition using property of the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition which has an anti-obesity action and is used as a supplement. <P>SOLUTION: The composition prevents obesity of an animal and contains a low-molecule polysaccharide. The composition is ingested together with food or forage so as that weight ratio of the food or the forage ingested by the animal to the low-molecule polysaccharide is 1:130 to 20:80, comprises the polysaccharide having approximately 16 pieces of D-glucose, and has a helical structure regarding homoglucose chain of the approximately 6 pieces of D-glucose as a unit. Preferably, the composition contains histidine, a bonito extract and dietary fiber further. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a composition comprising a low-molecular-weight polysaccharide having a helical structure and having an anti-obesity effect or a property of enhancing anti-obesity effect. The present invention also relates to a composition utilizing the anti-obesity action or anti-obesity action of this low molecular weight polysaccharide. The present invention further relates to a method for preventing obesity using these compositions.

  The anti-feeding action of proteins containing a large amount of histidine, for example, seafood proteins, is also elucidating the mechanism of action, and histamine produced by metabolism and derivatization of histidine in the body activates histamine neurons. It is said that it contributes to the prevention of overeating (Non-Patent Documents 1 to 12).

  For example, Non-Patent Document 1 suggests that high histidine-containing protein suppresses food intake from dietary surveys. Non-Patent Document 2 describes that ingested histidine is converted into histamine in the body and activates histamine neurons to show an eating suppression effect, and the tendency is more pronounced in women than in men. ing. Non-Patent Document 3 recognizes that there is a significant negative correlation between BMI and histidine intake per body weight. Further, Non-Patent Document 4 recognizes that when consuming seafood, the histidine intake per protein intake increases and a positive correlation is established. Non-Patent Document 5 reports that the lower the body fat percentage, the higher the histidine intake, and the reduction of body fat occurs due to the feeding suppression action and lipolysis action via nerve histamine, leading to obesity prevention. Non-Patent Document 6 reports that the weight gain was relatively minimal when histidine-rich boiled was given, based on the results of one-week rat breeding with casein, boiled and dried oysters. Non-patent document 7 reports that histidine absorbed in the body shows an antifeeding effect in about one day from a rat breeding experiment with a histidine diet (histidine 4 g / kg) and an arginine diet (arginine 4 g / kg). ing. Non-patent document 8 shows that, from a rat breeding experiment with a histidine diet (histidine 4 g / kg) and an arginine diet (arginine 4 g / kg), neutrality in liver tissue was observed, although no significant difference was observed in feed intake. Since the amount of fat showed a significantly low value in the histidine feed group, it is described that histidine may promote energy consumption. Non-Patent Document 9 reports that the concentration at which histamine develops an antifeedant action is 29 mg / g feed. Non-Patent Document 10 demonstrates the suppression of feeding and the promotion of lipolysis by a feed supplemented with a bonito hot water extract (histamine 37 mg / g).

Nakajima Shigeru, Tomita Kaoru et al .: Inhibition of eating by high histidine-containing protein observed in low-energy intakes, Nichiei / Food Journal 2000, 53: 207-214. Nakajima Shigeru, Tanaka Kaoru et al: Correlation between energy intake and histidine intake in women in the Seto Inland Sea area, obesity study 2001, 7: 276-282. Makiko Tsuji, Shigeru Nakajima et al .: Correlation between BMI and histidine intake, obesity study 2002, 8: 302-305. Nakajima Shigeru, Tanaka Kaoru et al .: Research study on histidine intake by food group supplying protein, obesity study 2004, 10: 66-72. Makiko Tsuji, Seiichi Kasaoka et al .: Correlation between body fat percentage and histidine intake per body weight, obesity study 2004, 10: 173-176. Nakajima Shigeru, Satoshi Hamada et al .: Inhibitory effect of niboshi on food intake. Fishers Science 2000, 66: 795-797. Nakajima Shigeru, Kasaoka Seiichi et al .: Antifeedant action of rats with histidine-added diet using the cafeteria method, obesity study 2002, 8: 55-60. Seiichi Kasaoka, Shigeru Nakajima et al .: Neutral fat lowering effect of rat liver tissue by diet containing histidine, obesity study 2002, 8: 168-172. Kasaoka S .; Kasaoka T et al .: Histidene supplementation supplements food intake and fat accumulation in rats. Nutrition 2004, 20: 991-996. Seiichi Kasaoka, Mika Nabeshima et al .: Suppressing eating and promoting lipolysis of histidine-rich substances obtained in the bonito production process, obesity study 2005, 1: 63-68.

  Despite the above effects of preventing overeating, histidine is metabolized and converted to histamine when ingested by the body. If histamine is excessive, allergic symptoms are manifested, and excessive histamine and allergic symptoms are manifested. It is shown that there is a significant correlation with. Therefore, it is desired to avoid the excessive intake of histidine and at the same time obtain the effect of preventing overeating using histidine.

  The present invention utilizes a low-molecular-weight polysaccharide having a helical structure prepared from, for example, starch, in order to solve the above-mentioned problems associated with overdose of histidine. The present inventors have found that a low-molecular-weight polysaccharide having a specific helical structure has an anti-obesity action or a property of enhancing the anti-obesity action, and has completed the present invention. This low-molecular-weight polysaccharide itself has an anti-obesity effect, and synergistically increases each other's anti-obesity effect by being present with histidine.

More specifically, the present invention can relate to the following items.
(Item 1) A composition for preventing obesity in an animal, comprising a low molecular weight polysaccharide, wherein the composition has a weight ratio of food or feed consumed by the animal and the low molecular weight polysaccharide of 1: Ingested with the food or feed in a ratio of 130 to 20:80, the low-molecular polysaccharide is composed of about 16 D-glucose, and about 6 D-glucose homoglucose chains as a unit. A composition having a helical structure.
(Item 2) The composition according to item 1, further comprising histidine, bonito extract or dietary fiber.
(Item 3) The composition according to item 2, comprising the histidine and the low molecular weight polysaccharide in a weight ratio of about 1:10 to about 1: 127.
(Item 4) The composition according to item 2, comprising the low molecular weight polysaccharide and the dietary fiber in a weight ratio of about 1: 1.
(Item 5) The composition according to any one of Items 1 to 4, which is a form selected from the group consisting of tablets, granule capsule preparations, and cookies.

  The low-molecular-weight polysaccharide is a homoglucose body comprising about 16 D-glucose, having a molecular weight of about 3,000, and having a helical structure having a unit of about 6 D-glucose homoglucose chains. It is water soluble and can be prepared, for example, from starch.

Preferably, the low molecular weight polysaccharide may have a right-handed spiral structure in which one turn of the spiral is composed of 6 D-glucose residues, and as a result, iodine molecule I ³ is incorporated into the center of the spiral to form iodine. It has a structure that develops color by a color reaction. Such a low-molecular-weight polysaccharide is commercially available as, for example, Potacogen (registered trademark) from Bizen Kasei Co., Ltd. (363 Tokutomi, Akamine, Okayama Prefecture).

  According to the present invention, a combination of histidine and a low-molecular-weight polysaccharide having a helical structure, typically Potacogen (registered trademark), exhibits a known anti-feeding effect and obtains an anti-obesity effect. The amount of histidine used can be reduced, and at the same time, the fat content of animal tissues can be reduced synergistically.

  This Potacogen (registered trademark) is also effective in reducing the subcutaneous fat of animals (lower abdominal subcutaneous fat; 19% lower, intraperitoneal fat; 24% lower) even in fructose-loaded rats using fructose, which is known to have a weight gain effect ), And the combined use of histidine and Potacogen (registered trademark) can be expected to reduce the amount of histidine used to obtain an anti-obesity effect and synergistically reduce the subcutaneous fat of animals. . Similarly, Potacogen (registered trademark) can be expected to act synergistically to reduce the amount of subcutaneous fat or body fat in animals by combining with dietary fiber such as okara. Therefore, the present invention can be applied to the elimination of obesity by applying it to animals including humans and pets.

  The water-soluble polysaccharide of the present invention itself has an anti-obesity action, and further, when used in combination with histidine, reduces the amount of histidine used to obtain an anti-obesity effect and synergizes body fat. To reduce. According to the present invention, a composite preparation or food composition with enhanced anti-obesity action is produced using this water-soluble polysaccharide.

  According to the present invention, a combination of anti-obesity histidine and Potacogen (registered trademark) produces a synergistic effect, reducing the amount of each component used to obtain the anti-obesity effect, and reducing body fat. The method for producing breads and confectionery such as tablets, granule capsule preparations or cookies shown can be applied to humans and pets to help maintain health.

  According to the present invention, in order to synergistically enhance the body fat reducing action of histidine, a food composition comprising a low-molecular polysaccharide and dietary fiber such as histidine or okara can be provided.

It is a figure which shows the anti-obesity effect of the composition of this invention. The vertical axis of the graph shown in the figure represents the lower abdominal subcutaneous fat content of the rats used in the experiment, and the horizontal axis of the figure represents the experimental group. From the left in the figure, the normal group, control group, test control group, PoG Each group is shown. It is a figure which shows the anti-obesity effect of the composition of this invention. The vertical axis of the graph shown in the figure represents the intraperitoneal white fat content of the rats used in the experiment, and the horizontal axis of the figure represents the experimental group. From the left in the figure, the normal group, the control group, the test control group, PoG Each group is shown.

  The term “anti-obesity effect” as used herein, when taken by an animal as food or feed, or as part of food or feed, properly controls the animal's food intake and, as a result, It refers to an action that suppresses or decreases the increase in body weight, or an action that suppresses or decreases the increase in the body fat or the subcutaneous fat content of tissues of this animal. The composition of the present invention can be ingested usually as an ingestible feed or food supplement (also referred to herein as “foods or food additives”).

  Preferably, the composition of the present invention is used as a feed or food supplement to be ingested, and the composition has a weight ratio of food or feed to be consumed by the animal and the low molecular weight polysaccharide of from 1: 130 to Ingested with the food or feed at a ratio of 20:80.

  Preferably, the composition of the present invention comprises the histidine and the low molecular weight polysaccharide in a weight ratio of about 1: 5 to about 1: 127, more preferably the composition of the present invention comprises It contains histidine and the low molecular weight polysaccharide in a ratio of about 1:10 weight ratio.

  Also preferably, the composition of the present invention comprises the low molecular weight polysaccharide and the bonito extract or dietary fiber in a proportion by weight ratio ranging from about 1: 2 to 2: 1, more preferably The composition of the invention comprises the low molecular weight polysaccharide and the bonito extract or dietary fiber in a weight ratio of about 1: 1. In addition, when mentioning% in this specification, unless otherwise specified, it means weight%.

  The “animal” to which the composition of the present invention is applied includes, without limitation, primates, mammals, reptiles, amphibians or birds, pet animals, livestock and the like.

  An effective amount of the anti-obesity effect of the composition of the present invention can be readily ascertained by a suitable mammalian model. Such information can then be used to determine further useful amounts for ingestion in humans. The “effective amount for exhibiting an anti-obesity effect” in humans can be easily determined according to, for example, experiments using rats described in the present specification. Alternatively, since the composition of the present invention does not contain harmful components, the “effective amount for showing an anti-obesity effect” in humans can be easily confirmed by ingestion by humans.

  The amount of the low molecular weight polysaccharide, the low molecular weight polysaccharide, and the histidine, bonito extract or dietary fiber, which are actually taken with feed or food and exhibit anti-obesity action, depends on the weight, health condition, etc. of the applied individual. And can be adjusted so that the anti-obesity effect is optimally achieved.

  The tablet and granule capsule preparation can be prepared using a tableting aid, granule aid, and capsule aid.

  The term “tablet processing aid” used in the present specification is not limited, and includes any sugars such as granulated sugar, super white sugar, powdered sugar, reduced maltose starch syrup, lactose, glucose, pullulan, erythritol, starch, dextrin, Dietary fibers such as crystalline cellulose, gum arabic, and okara, food calcium such as corn protein and calcium phosphate, food extracts, food dried powders, natural fruit juice powders, surfactants such as sucrose fatty acid esters, powdered fats and oils , Oils and fats such as glycerin, fatty acid esters, etc., various sweeteners used in chewable tablets (eating tablets), various acidulants, various flavoring materials such as fragrances, coating materials or shellac, corn protein, yeast cell wall, starch, Reduced maltose starch syrup, sugarless sugar coating, maltitol, guri Including phosphorus, sorbitol, HPMC, the HPC.

  The term “granular processing aid” used in the present specification is not limited, and includes any sugar, crystals such as granulated sugar, super white sugar, powdered sugar, reduced maltose starch syrup, lactose, glucose, pullulan, erythritol, starch, and dextrin. Includes dietary fibers such as cellulose and gum arabic, food calcium such as corn protein and calcium phosphate, food extracts, food dry powders, and natural fruit juice powders.

  The term “capsule processing aid” as used herein is not limited, and is used to prepare hard capsule type capsules, granulated sugar, super white sugar, powdered sugar, reduced maltose starch syrup, lactose, glucose, pullulan, All sugars such as erythritol, starch, dextrin, dietary fibers such as crystalline cellulose and gum arabic, food calcium such as corn protein and calcium phosphate, food extracts, dried food powders, natural fruit juices, and soft capsule type capsules It contains content viscosity modifiers such as food fats and oils, beeswax and glycerin fatty acid esters for preparation.

  A tableting formulation is usually prepared using a tableting machine. A tableting preparation is blended with a seasoning material to form a chewable tablet, or the tablet surface is coated using an automatic coating machine, a spray granulator or a hand pan. Various granulators of the spray granulator type, the kneading (extruding) type, or the high-speed stirring granulator type can be used for forming the granule preparation. For preparation of capsule preparation, capsule filling machines (hard type and soft type) can be used by mixing capsule aids.

  The invention is illustrated using the examples described below. The following examples are not intended to limit the invention, but are merely illustrative of the invention.

Example 1 Anti-obesity effect of low molecular weight polysaccharide
1. Sample, feed, and animal MF standard feed (Oriental Yeast Industry Co., Ltd.) were added with 20% Potacogen (registered trademark) to prepare a sample. SD rats (female, 7 weeks old) were purchased from Nippon Charles River Co., Ltd.).

2. Experimental Method Three groups of 6 SD female rats were prepared. The control group and Potacogen (registered trademark) administration group (experimental group) were allowed to freely take a 25% fructose aqueous solution and the normal group as tap water for 4 weeks. Meanwhile, 20% Potacogen (registered trademark) -containing diet was freely given to the experimental group, and a standard diet was freely given to the control group and normal group.

3. Experimental Results From the 15th day after the start of breeding, the body weight of the experimental group showed a slightly decreasing tendency compared to the control group. In the control group and the experimental group, the average daily diet per rat during the 27-day breeding period was 9.27 g, which was less than the average daily diet of the normal group, which was about 14 g. The average daily intake of fructose was 23-25 mL / day / animal although the control group was slightly higher than the experimental group.

  On the final day of the experiment, the rats were sacrificed, and the lower abdominal subcutaneous fat and intraperitoneal fat were removed and their weights were measured and compared. As a result, the experimental group had 24.8% lower abdominal subcutaneous fat and 19.3% less intraperitoneal white fat than the control group. From this result, it is clear that Potacogen (registered trademark) has an anti-obesity effect, exhibiting a weight-reducing action and an action to reduce subcutaneous fat content even in the presence of fructose, which is known to have a weight-gaining action. It was.

(Example 2)
1. Sample, feed and animal Potacogen (registered trademark) 50%, L-histidine 5%, L-citrulline 7.5%, powder Mabit 50M 35.5%, sugar ester 1% and tribasic calcium phosphate 1% , PoG-His blended powder) was prepared and used as a specimen.

  High fat high sucrose diet (beef tallow, lard fat content 30%, Oriental Yeast Co., Ltd.), MF Standard Feed (Oriental Yeast Industry Co., Ltd.) and SD female rats (7 weeks old, Charles River Japan ( Co.)) was purchased and prepared.

2. Experimental Method SD female rats were divided into 4 groups (5 each), high fat high sucrose diet for the control group and high fat high sucrose diet for the test control group so that the rat MF standard diet was 10%. As a PoG group, 10% of a PoG-His compound powder mixed with a high-fat and high-sucrose diet was fed as a PoG group, and a rat MF standard diet as a normal group, and the following items ( 1-4) were tested. In 1 g of feed of the PoG group, 50 mg of Potacogen (registered trademark) and 5 mg of L-histidine are contained.
(1) Body weight was measured for 39 consecutive days every 3 days after the start of the experiment and after the start of the experiment.
(2) On the last day of the experiment, the lower abdominal subcutaneous fat and intraperitoneal white fat were excised at the sacrifice of the rat, and these fats were measured.
(3) As the food intake of each group, the average intake per rat was calculated every three days from one week before the start of the experiment to the end of the experiment.
(4) Statistical analysis was performed by Dunnett, t-test for the difference between the mean values, and p <0.05 and p <0.01 were significant differences * and **, respectively.

3. Results Rats in the high-fat high-sucrose diet group (control group) gained an average weight gain of 2.5 times or more on average compared to rats in the MF standard diet group (normal group) by breeding for 39 days. Indicated. And the high fat high sucrose diet administration group (test control group) to which 10% MF standard feed was added, although the average body weight was reduced by about 11% from the high fat high sucrose diet administration group (control group), About 26% weight loss was observed in the high fat high sucrose diet administration group (PoG group) added with 10% PoG-His.

  As a result of examining the body fat content as the contents of the weight loss, it was as follows. That is, the lower abdominal subcutaneous fat content of the PoG group was reduced by about 34% on average from that of the test control group (FIG. 1). In addition, the intraperitoneal white fat content in the PoG group decreased by about 48% on average from that in the test control group (FIG. 2). Thus, it was clarified that administration of a high-fat high-sucrose diet added with a PoG-His compound powder significantly reduced the increase in rat lower abdominal subcutaneous fat and intraperitoneal white fat. The average daily intake of rat food is about 16.4 g in the normal group, while it is about 14 g in both the control group and the experimental group of the test control group. In the high fat high sucrose diet group, Antifeedant effect was observed. This is probably because the high-fat and high-sucrose diet has higher energy than the MF standard feed.

(Example 3)
1. Analyte, 24.47% flour as a base feed and animal cookies, 10% sugar, unsalted butter 31.55%, sweetener (stevia) 0.1%, trehalose 5.62%, whole eggs 5.26% , 3.85% dextrin was mixed. Separately, Potacogen (registered trademark) and bonito extract (containing histidine: prepared as described below) and okara (commercially available at supermarkets, etc.) were mixed at a ratio of 150: 75: 150. Sample B was prepared by mixing sample A, Potacogen (registered trademark) and okara in a ratio of 150: 150. Samples A and B were each made into a cookie of Sample I and Sample II by adding 19.15% to the above-mentioned base to give a total of 100%.

-Preparation method of bonito extract-
500 g of flower bonito was placed in 4 L of distilled water, boiled, and extracted by heating for 2 hours. After cooling, the filtrate was collected by filtration. 4 L of distilled water was again added to the filtered flower bonito and boiled, followed by heating and extraction for another hour. After allowing to cool, the filtrate was collected in the same manner, and the filtrate was collected. The filtrate was combined with the previous filtrate, and then concentrated under reduced pressure to 240 mL. Ethanol was added to the obtained concentrated liquid, and the resulting precipitate was collected by filtration to obtain a skipjack extract (11.4 g). A part of the precipitate was dissolved in water and thin-layer chromatography (developing solution; developed with n-butanol: glacial acetic acid: ethanol: distilled water = 4: 1: 1: 2 to confirm histidine spots).
Samples I and II were pulverized and added to a high-fat high-sucrose diet at a constant rate. The histidine content in Sample I was 0.6 mg / g as a result of measurement using an amino acid automatic measuring instrument. Potacogen (registered trademark) is contained in 16.6 g of Sample I at 76.6 mg.

  High fat high sucrose diet (beef tallow, lard fat content 30%, Oriental Yeast Industry Co., Ltd.), MF Standard Feed (Oriental Yeast Industry Co., Ltd.) and SD female rats (6 weeks old, Charles River Japan ( Co.)) was purchased and prepared.

2. Experimental Method SD female rats were divided into 4 groups (6 rats each), and the control group was mixed with a high fat and high sucrose diet so that the rat MF standard diet was 10%, and was freely ingested. In addition, the sample cookie of sample I and sample II was ground and then mixed with 10% high fat and high sucrose diet as the experimental group, and the normal group was allowed to freely ingest the rat MF standard diet. Items (1-4) were tested.
(1) The body weight was measured for 6 consecutive weeks on the start date of the experiment and every other week after the start of the experiment.
(2) The rats were sacrificed on the last day of the experiment, and the lower abdominal subcutaneous fat and intraperitoneal white fat were removed and their weights were measured.
(3) The food intake of each group was calculated as the average intake per rat every three days from one week before the start of the experiment to the end of the experiment.
(4) Statistical analysis was performed by Dunnett, t-test for the difference between the mean values, and p <0.05 and p <0.01 were significant differences * and **, respectively.

3. Results The anti-obesity effect of cookies containing Potacogen (registered trademark) and histidine on rat body fat accumulation by administration of a high-fat and high-sucrose diet was examined.

  Rats in the high fat and high sucrose diet group (control group) showed an average weight gain of about 2.3 times that of rats in the MF standard feed group (normal group) after 6 weeks of breeding. It was. In contrast, in the experimental group to which Sample I and Sample II were added, an average of 34.3% and 27.8% weight loss was observed, respectively, compared with the control group of rats. In addition, the change in the abdominal subcutaneous fat content was found to decrease by 39.2% and 31.7% on average in the rats of the cookie addition experimental group of Sample I and Sample II, respectively, as compared with the rats of the control group. It was. The changes in intraperitoneal white fat were significantly decreased in the samples of the sample I and sample II cookie-added experimental groups, on average, 54.9% and 49.3%, respectively, as compared with the rats in the control group. .

  Thus, it has been clarified that the intake of the high fat and high sucrose diet to which the cookies of Sample I and Sample II are added significantly reduces the increase in rat lower abdominal subcutaneous fat and intraperitoneal white fat.

  In addition, from the results of the cookie addition experiment group of Sample II, the amount of subcutaneous fat in the lower abdomen and the amount of white fat in the abdominal cavity of the rat decreased, so that combination of Potacogen (registered trademark) and okara also increased body fat. It became clear that it decreased significantly. The average daily intake of rat food is about 16 g in the normal group, and about 14 g in both the control group and the experimental group, and the high-fat and high-sucrose diet group has an antifeeding effect. It was.

Example 4
Potacogen (registered trademark): L-histidine: L-citrulline = 10: 1: 1.5 ratio mixture was made and mixed powder A was obtained. 62% of mixed powder A, 35.5% of reduced maltose starch syrup powder, 1% of sucrose fatty acid ester and 1% of tribasic calcium phosphate were mixed to form a tableted product having a diameter of 8 mm and 250 mg / grain. The numerical value of one grain was an average weight of 249.7 g / grain, an average tablet hardness of 12.5 kg / cm ² , and an average tablet thickness of 4.9 mm.

  Tableting is possible at an addition amount of the mixed powder A of 95% to 10%, and the content can be controlled according to the weight shape of the tablet. The calculated contents of Potacogen®, L-histidine and L-citrulline in one tablet are 124 mg, 12.4 mg and 18.6 mg, respectively.

(Example 5)
34% of mixed powder A, reduced maltose starch syrup powder 25%, trehalose 10%, acidulant 5%, high sweetener 0.2% milk calcium 3%, fruit flavor 1%, fruit juice powder 3%, dextrin 48.8% The granulation was carried out using a spray granulator at a weight ratio of 5 and water as the spray liquid. This product was able to produce fruit-type granules with good fluidity and sourness and sweetness. The mixed powder A can be spray granulated by adding 90 to 10% of a paste solution as a thickener at a content of 1% to 90%.

(Example 6)
Capsule tablets were produced with a hard capsule filling machine of a mixture of mixed powder A 98%, tricalcium phosphate 1% and sucrose fatty acid ester 1%.

(Example 7)
A soft capsule-filled preparation was obtained by mixing 37% of mixed powder A, 55% vegetable oil, and 8% beeswax glycerin fatty acid ester.

(Summary)
1. First, the fat reducing effect of Potacogen (registered trademark) was examined in Example 1. As a result, in the experimental group in which 25% fructose aqueous solution was ingested freely during the 4-week rearing period, in the group of rats fed with a diet containing 0.2 g of Potacogen (registered trademark) / 1 g of diet, Potacogen (registered trademark) ), The lower abdominal subcutaneous fat was reduced by 24.8% and the intraperitoneal white fat was reduced by 19.3%. From these results, it was clarified that Potacogen (registered trademark) exhibits an effect of reducing body weight and an effect of decreasing the subcutaneous fat content of animal tissues even in the presence of fructose having an effect of increasing body weight.

  The mechanism of reducing the fat of Potacogen® is that the administration of Potacogen® increases insulin, acts on the insulin-sensitive hormone leptin, activates AMP kinase, and activates AMP kinase. Regulates lipid metabolism (Minokoshi, Y et al .: Leptin stimulated fatty acidification by activating AMP-activated protein kinase, Nature, 415: 339-343, 2002). .

  2. Whether or not a combination of histidine and Potacogen (registered trademark) exhibits a body fat reduction effect when the amount of histidine used is lower than the 29 mg / g expression level of histamine feeding suppression described in Non-Patent Document 9. Examined (Example 2). In Example 2, SD female rats were divided into 4 groups (5 each), the control group was mixed with a high fat high sucrose diet, and the test control group was mixed with a 10% high fat high sucrose diet. And allowed to ingest freely. In addition, a diet in which 10% of the mixed powder of histidine and Potacogen (registered trademark) as a sample was mixed with a high-fat high-sucrose diet was used as the PoG group. Rats were raised for 39 consecutive days and sacrificed, and body weight, abdominal subcutaneous fat content, and abdominal subcutaneous fat content were measured. Potacogen (registered trademark) 50 mg and L-histidine 5 mg are contained in 1 g of feed of the PoG group (Example 2). Potacogen (registered trademark) 50 mg and L-histidine 5 mg have a ratio of 10: 1, but the ratio is not limited.

  Rats in the high fat high sucrose diet group (control group) showed a 2.5-fold increase in body weight after 39 days of breeding compared to rats in the MF standard feed group (normal group). The 10% MF standard feed-added high fat high sucrose diet administered group (test control group) was 10% PoG-, although the body weight decreased by about 11% compared to the high fat high sucrose diet administered group (control group). In the high fat high sucrose diet administration group (PoG group) to which the His compound was added, weight loss of about 26% was observed. As a result of examining the fat content as the contents of the weight loss, it was as follows. That is, the abdominal subcutaneous fat content of the PoG group was reduced by about 34% from that of the test control group. In addition, the intraperitoneal white fat content in the PoG group was reduced by about 48% from that in the test control group. Thus, it was revealed that administration of a high-fat and high-sucrose diet containing PoG-His compounded powder significantly reduced the increase in rat lower abdominal subcutaneous fat and intraperitoneal white fat.

  As a result, 1 mg of bait contains 50 mg of Potacogen (registered trademark) and 5 mg of L-histidine. This is lower than the Potacogen (registered trademark) content 0.2 g / g 1 g shown in Example 1, and the histamine feeding inhibitory expression level described in Non-Patent Document 9 is lower than 29 mg / g. It shows body fat reduction effect. This revealed that the combination of histidine and Potacogen (registered trademark) has a synergistic effect on body fat reduction.

  3. Furthermore, in order to clarify the synergistic effect of histidine and Potacogen (registered trademark), the effect of rats with histidine and Potacogen (registered trademark) on rats (bred for 6 weeks) was examined and the following results were obtained. (Example 3).

  The rats in the high fat and high sucrose diet group (control group) showed about 2.3-fold increase in body weight after breeding for 6 weeks compared with the rats in the MF standard diet group (normal group). In contrast, a histidine content of 0.6 mg / g per 1 g of feed and Potacogen (registered trademark) of 76.6 mg content showed a weight loss of 34.3% that of the control group. The changes in the abdominal subcutaneous fat content and intraperitoneal white fat were 39.2% and 54.9%, respectively, in the control group, showing a significant decrease. The combination of histidine and Potacogen (registered trademark) reduced body fat. It was revealed that both histidine and Potacogen (registered trademark) show a synergistic effect at a low content.

  The bait of Example 3 was used for the purpose of increasing the amount of the high content of dietary fiber from the waste for the purpose of waste utilization, but by using a cookie in which 76.6 mg of Potacogen (registered trademark) and okara were added to 1 g of bait. A weight loss of 27.8% of that of the control group was observed. In addition, changes in the abdominal subcutaneous fat content and intraperitoneal white fat were significantly reduced to 31.7% and 49.3% in the control group, respectively. This revealed that the combination of Potacogen (registered trademark) and okara was also effective in reducing body fat in rats. It is also conceivable to use dietary fibers containing water-soluble plant fibers such as crystalline cellulose, gum arabic and alginic acid instead of okara.

  Compositions having anti-obesity activity are provided. This composition can be added as a feed or food supplement and applied to animals including humans and pets to help eliminate obesity.

Claims (5)

A composition for preventing obesity of an animal, comprising a low molecular weight polysaccharide, wherein the composition has a weight ratio of food or feed consumed by the animal and the low molecular weight polysaccharide of 1: 130 to 20: Ingested with the food or feed in a ratio of 80, the low-molecular-weight polysaccharide is composed of about 16 D-glucose, and has a helical structure with about 6 D-glucose homoglucose chains as a unit. Having a composition. The composition of claim 1 further comprising histidine, bonito extract or dietary fiber. 3. The composition of claim 2, comprising the histidine and the low molecular weight polysaccharide in a weight ratio of about 1:10 to about 1: 127. The composition of claim 2, comprising the low molecular weight polysaccharide and the dietary fiber in a weight ratio of about 1: 1. The composition of any one of Claims 1-4 which is a form selected from the group which consists of a tablet, a granular capsule formulation, and a cookie.

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