JP4698236B2 - Hair growth cosmetics - Google Patents

Description

  The present invention relates to a hair-growth cosmetic characterized by blending a plurality of amino acids constituting a storage region of keratin-associated protein which is a hair structure factor.

  In the past, ingredients that have a hair-growth effect that prevent or suppress hair loss and promote hair growth and hair growth are searched for, for example, vasodilators such as assembly extract, vitamin E acetate, minoxidil, stimulants such as chili tincture, Vitamins such as pantothenic acid and biotin, hormone agents, anti-inflammatory agents, bactericides, and the like have been used.

On the other hand, keratin which is the main component of hair contains 20 kinds of amino acids shown in the table below. It has been pointed out that lack of these amino acids leads to hair root nutritional deficiency, which causes hair loss.

  There are many examples of hair restorers that contain amino acids. However, in many patents it is common to use amino acids as humectants rather than as ingredients with hair-growth effects. Among them, examples in which amino acids and higher fatty acids, higher alcohols, adducts, N-acylamino acids, tea extracts, tannins, soybean saponins, peppers, citrus fruits and the like are used in combination have been reported. (See Patent Documents 1-7)

  One report is known as an example of a hair restorer composed of an amino acid alkyl ester or an ester salt of cysteine, glutamic acid, and arginine essential as active ingredients. (See Patent Document 8)

Furthermore, although there is an example of a cosmetic (see Patent Document 9) in which nine types of amino acids are blended as a mixture, amino acids are blended as a moisturizing agent.
Japanese Patent Laid-Open No. 5-286634 JP-A-6-227949 JP 7-131644 A JP-A-9-175945 Japanese Patent Laid-Open No. 10-059829 Japanese Patent Laid-Open No. 11-363035 JP 2004-143129 A JP 2002-363035 A JP-A-9-87126

  To provide a hair-growth cosmetic that exhibits an excellent hair-growth effect.

  It is surprising that a group of amino acids derived from keratin-associated protein, which is a hair structure factor, was formulated with a plurality of amino acids, which are components derived from keratin-associated protein, which is a hair structure factor, based on the knowledge that it has a hair-growth effect on human hair. Has also been found to have a hair-growth effect.

  As a result of examining the cell activation effect of the keratin-associated protein-derived amino acid mixture, which is a hair structure factor, on human hair matrix cells that have been separated and cultured, the keratin-associated protein-derived amino acid mixture-treated cell, which is a hair structure factor, is compared with untreated cells. A significant cell activation effect was observed. In addition, as a result of investigating the effects of keratin-associated protein-derived amino acid mixed preparations, which are hair structure factors, on human hair growth, a significant increase in hair growth rate was achieved by continuously applying the keratin-associated protein-derived amino acid mixture, which is a hair structure factor. Was observed.

The present inventors identified a peptide derived from a hair structure factor as an ingredient having a hair-growth effect, and identified an amino acid optimal composition in which hair-growth action was observed in collaboration with Keio University, and identified the polypeptide as a high sulfur type, It was roughly divided into three groups: high glycine / tyrosine type and high glycine / systemine / serine type. The contents were as shown in the table below.

  In these three groups, highly conserved amino acid sequences appeared repeatedly, and the physiological activity of the design peptides composed of these conserved amino acid sequences was investigated, but the function of the keratin-associated protein was not clear so far. The inventors have found that a peptide derived from a conserved sequence present in a protein associated with keratin has a growth promoting action for increasing the hair growth rate on mouse hair, and the result (“Japanese Patent Application No. 2001-348050”) "Novel hair keratin-associated protein" and "Japanese Patent Application No. 2003-393014", title of the invention "cell activator, hair growth agent, and hair growth promoter").

  However, in application to human hair restorers for humans, there is no general versatility in terms of cost in the peptide state, and there is room for improvement in percutaneous penetration due to the molecular weight being too large. A mixture of amino acids was prepared at a composition ratio equivalent to the amino acid sequence constituting this design peptide, and the effect on human hair growth was studied.

  That is, in the three groups constituting the design peptide, for the amino acid mixture with reference to high sulfur type, the molar ratio of serine, cysteine, alanine, valine, lysine, arginine, glutamine, proline is 7: 11: 1: The amino acid mixture was prepared at a ratio of 1: 1: 1: 4: 4, and the amino acid mixture with reference to high glycine / tyrosine type was adjusted to have a molar ratio of glycine, tyrosine, and cysteine of 8: 5: 2. A mixture was prepared, and an amino acid mixture was prepared at a molar ratio of glycine, serine, cysteine, and lysine of 16: 7: 6: 1 with respect to the amino acid mixture with reference to high glycine / systemine / serine type.

  The amount of the amino acid mixture of three types, namely high sulfur type, high glycine / tyrosine type, and high glycine / cysteine / serine type, is 0.0001 to 20% by weight. More preferably, it is 0.001 to 10% by weight.

In addition to the above components, the components used in the hair-growth cosmetics within a range that does not impair the effects of the present invention, such as oils, higher alcohols, fatty acids, ultraviolet absorbers, surfactants, polyhydric alcohols / sugars, polymers, Bioactive ingredients, antioxidants, solvents, fragrances, preservatives, and the like can be blended.
Specific examples are shown below.

(1) Examples of oil components Ester-based oil phase components: cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, myristic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate, isoaralkyl neopentanoate, tri 2-ethylhexanoic acid trimethylolpropane, triisostearic acid trimethylolpropane, tetra-2-ethylhexanoic acid pentaerythritol, capryl Cetyl, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl myristate, iso myristate Stearyl, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetostearyl 2-ethylhexanoate, 2-ethylhexanoic acid Stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene dicaprate Glycol, di (caprylic / capric) propylene glycol, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, octyldodecyl neopentanoate, isostearyl octoate, octyl isononanoate, hexyldecyl neodecanoate, neodecanoic acid Octyldodecyl, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, dipropyl carbonate, dialkyl carbonate (C12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, Cetyl lactate, octyl decyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, citric acid Trioctyl, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate , Dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, isostearyl 12-stearoylhydroxystearate, and the like.
Animal and vegetable oils and their hydrogenated oils, and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated tallow, horse oil, hydrogenated horse oil, mink oil, orange luffy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils and Its hardened oil, avocado oil, almond oil, olive oil, cacao butter, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, camellia oil Corn oil, rapeseed oil, hardened rapeseed oil, palm kernel oil, hardened palm kernel oil, palm oil, hardened palm oil, peanut oil, hardened peanut oil, castor oil, hardened castor oil, sunflower oil, grape seed oil, jojoba oil , Hardened jojoba oil, macadamia nut oil, medhome oil, cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil and other vegetable oils and their hardened oils, beeswax, high acid value beeswax, la Phosphorus, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax and montan wax.
Silicone oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, dimethylsiloxane / methylcetyloxysiloxane Examples thereof include a polymer, a dimethylsiloxane / methylstearoxysiloxane copolymer, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicic acid, and silicone RTV rubber.
Fluorine-based oil phase components: perfluoropolyether, fluorinated pitch, fluorocarbon, fluoroalcohol and the like.

(2) Examples of higher alcohols Lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol and the like can be mentioned.

(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid , Erucic acid, 2-ethylhexanoic acid and the like.

(4) Examples of ultraviolet absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyldihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene glycol salicylate, salicylic acid Octyl, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate, mono 2-diparamethoxycinnamate Glyceryl ethylhexanoate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamate, diisopropyl diisopropylke Cinnamic acid ester mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetra Hydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2- (2-hydroxy-5-methylphenyl) ) Benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate, phenol Rubenzimidazole sulfate, 3- (4-methylbenzylidene) camphor, isopropyldibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like, and These polymer derivatives, silane derivatives and the like can be mentioned.

(5) Examples of surfactants Anionic surfactants: fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate , Alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl amide phosphate, alkyloyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetic acid Examples thereof include sodium, acyl isethionate, acylated hydrolyzed collagen peptide salt, and perfluoroalkyl phosphate.
Cationic surfactant: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride , Behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, and the like. Moreover, tertiary amines, such as fatty acid amide dialkylamine, and its salt are also mentioned.
Amphoteric surfactants: carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type and the like.
Nonionic surfactant: propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbit fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE cured castor Oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid and the like.
Natural surfactant: lecithin, saponin, sugar surfactant and the like.

(6) Examples of polyhydric alcohols and sugars Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3 -Butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan and the like. These chemically modified compounds can also be used.

(7) Examples of physiologically active ingredients Examples of physiologically active ingredients include substances that impart some physiological activity to the skin when applied to the skin. For example, whitening, anti-inflammatory, anti-aging, UV protection, slimming, squeezing, anti-oxidation, hair growth / hair growth, moisturizing, blood circulation promotion, antibacterial / sterilization, cold / warm feeling, promotion of wound healing, stimulation relaxation, analgesia, cells It is a component having effects such as activation, and examples thereof include plant extracts, seaweed extracts, vitamins and derivatives thereof, biopolymers such as amino acids, peptides, mucopolysaccharides, enzyme components, and the like. Examples of these suitable ingredients include, for example, Ashitaba extract, avocado extract, amateur extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, isoflavone, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract. , Ages extract, Echinacea leaf extract, Ogon extract, Oat extract, Auren extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Cacao extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat Powder, hydrolyzed silk, pumpkin seed extract, chamomile extract, carrot extract, cormorant extract, licorice extract, calcade extract, oyster extract, kiwi extract, kina extract, cuecan -Extract, Guanosine, Gardenia extract, Kumazasa extract, Clara extract, Cranberry extract, Walnut extract, Grapefruit extract, Clematis extract, Chlorella extract, Mulberry extract, Gentian extract, Black tea extract, Yeast extract, Burdock extract, Rice bran extract, Rice germ oil, Comfrey extract, Collagen, Cowberry extract, Saishin extract, Psycho extract, Saitai extract, Salvia extract, Soap extract, Sasa extract, Hawthorn extract, Salamander extract, Shiitake extract, Giant extract, Shikon extract, Perilla extract, Linden extract, Shimotake extract Peonies extract, ginger root extract, birch extract, horsetail extract, horseshoe extract, hawthorn extract, elderberry extract, cereal Achillea millefolium extract, Atlantic mint extract, sage extract, mallow extract, senkyu extract, assembly extract, soybean extract, taisou extract, fermented soybean extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, evening primrose extract, cinnamon extract, terminaria Extract, Toki Extract, Toki-Senka Extract, Tonin Extract, Spruce Extract, Dokudami Extract, Tomato Extract, Natto Extract, Carrot Extract, Garlic Extract, Novara Extract, Hibiscus Extract, Bacmond Extract, Parsley Extract, Honey, Banana Flower Extract, Hamamelis Extract , Parietalia extract, toad extract, bisabolol, loquat extract, dandelion extract, tomato extract, bukkuri extract, butcher bloom extract Grape extract Examples include Yuzu extract, Yakuinin extract, Artemisia extract, Lavender extract, Apple extract, Lychee extract, Lettuce extract, Lemon extract, Astragalus extract, Rose extract, Rosemary extract, Roman chamomile extract, Royal jelly extract and the like.

  In addition, biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membrane, amino acids other than those used in the present invention, hydrolyzed peptides, lactic acid Moisturizing ingredients such as sodium, urea, sodium pyrrolidonecarboxylate, betaine, whey, trimethylglycine, lysine / arginine condensate, oily ingredients such as sphingolipid, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, hydrocortisone, tea tree oil and other anti-inflammatory agents, vitamin A and its derivatives, vitamin B2 and its derivatives Body, vitamin B6 and its derivatives, vitamin C and its derivatives, vitamin D and its derivatives, vitamin E and its derivatives, vitamins such as calcium pantothenate, biotin, nicotinamide, allantoin, diisopropylamine dichloroacetate, 4-amino Active ingredients such as methylcyclohexanecarboxylic acid, antioxidants such as tocopherols, carotenoids, flavonoids, tannins, lignans, saponins, cell activators such as α-hydroxy acids, β-hydroxy acids, γ-oryzanol, vitamin E derivatives, etc. Blood circulation promoters, wound healing agents such as retinol and retinol derivatives, arbutin, kojic acid, placenta extract, whitening agents such as sulfur, ellagic acid, linoleic acid, tranexamic acid, glutathione, cephalanthin, licorice extract, red pepper Tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivative, calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, Allantoin, isopropylmethylphenol, estradiol, ethinylestradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, takanal, camphor, salicylic acid, nonyl acid vanillylamide, nonanoic acid vanillylamide, piroctone olamine, pentadecanoic acid glyceryl, L-menthol, mono Nitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, canta Suchinki, cyclosporine, zinc pyrithione, hydrocortisone call Chizon, minoxidil, polyoxyethylene monostearate sorbitan, peppermint oil, and the like hair-growing agent such Sasanishiki extract.

(8) Examples of antioxidants Sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolyl biguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxy Examples include plant extracts having an antioxidant effect such as toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoid, flavonoid, tannin, lignan, saponin, apple extract and clove extract.

(9) Examples of solvents Purified water, ethanol, lower alcohol, fluoroalcohol, volatile linear silicone and the like can be mentioned.
Examples of application of the hair growth cosmetic of the present invention include hair creams, lotions, hair essences, shampoos, rinses, hair spray type hair cosmetics, and the like.
As the dosage form of the external preparation for skin of the present invention, conventionally known dosage forms such as bilayer, water-in-oil emulsion, oil-in-water emulsion, gel, spray, mousse, and oil can be used.
EXAMPLES The present invention will be described more specifically with reference to examples below, but the technical scope of the present invention is not limited to these examples.

(1) Evaluation Method and Evaluation Criteria Test Example 1. Maternal cell activation test

1-1. Exam overview
The activation effect of the amino acid mixture derived from keratin-associated protein, which is a hair structure factor, on human hair matrix cells that have been cultured separately is evaluated.

1-2. experimental method
Maternal cells separated and cultured from human hair removed were seeded in a 96-well microplate so as to be almost confluent using Dulbecco's modified MEM (DMEM / 5) containing 5% calf serum (FBS). 24 hours after sowing, the medium was replaced with 1% FBS-containing DMEM (DMEM / 1) containing an amino acid mixture having a predetermined concentration. After culturing for 48 hours using sample-containing DMEM / 1, the culture medium was replaced with DMEM / 1 containing 0.4 mg / mL MTT and cultured for 2 hours. After removing the medium, the amount of MTT reduction was determined by measuring the absorbance at 550 nm of the cell lysate dissolved by adding 2-propanol.
The amount of MTT reduction was expressed as a percentage with the absorbance of the cultured cells (control) to which the sample was not added being 100, as an activation effect (%).

1-3. result
From the results in Table 1, a significant cell activation effect in the amino acid mixture-treated cells was recognized as compared with untreated cells.

試験例２．毛乳頭細胞の賦活作用試験

Test Example 2 Test of activation of hair papilla cells

2-1. Exam overview
The activation effect of the amino acid mixture derived from keratin-associated protein, which is a hair structure factor, on human hair papilla cells is evaluated.

2-2. experimental method
Papilla cells were seeded in a 96-well microplate so as to be almost confluent using Dulbecco's modified MEM (DMEM / 5) containing 5% calf serum (FBS). 24 hours after sowing, the medium was replaced with 1% FBS-containing DMEM (DMEM / 1) containing an amino acid mixture having a predetermined concentration. After culturing for 48 hours using sample-containing DMEM / 1, the culture medium was replaced with DMEM / 1 containing 0.4 mg / mL MTT and cultured for 2 hours. After removing the medium, the amount of MTT reduction was determined by measuring the absorbance at 550 nm of the cell lysate dissolved by adding 2-propanol.
The amount of MTT reduction was expressed as a percentage with the absorbance of the cultured cells (control) to which the sample was not added being 100, as an activation effect (%).

2-3. result
From the results in Table 2, a significant cell activation effect in the amino acid mixture-treated cells was recognized as compared with untreated cells.

毛髪は主として毛母細胞および毛乳頭細胞より構成されており、これら２つの細胞の賦活化は、毛髪のサイクル、特に成長に対して作用することがわかる。
試験例３．ヒト育毛試験

It can be seen that hair is mainly composed of hair matrix cells and dermal papilla cells, and activation of these two cells acts on the hair cycle, particularly growth.
Test Example 3 Human hair growth test

3-1. Outline of the test The effect of the keratin-associated protein-derived amino acid mixed preparation, which is a hair structure factor, on human hair growth is evaluated.

3-2. Create a frame of about 1 cm x 1 cm on the side of the experimental method panel (as close to the top of the head as possible), capture the image of the shaved part with a microscope, and capture the same part with the microscope again three days later. It is. For the same hair, the length of the hair grown in 3 days was measured, and the hair growth rate per day was calculated (unit: mm / day). The sample was applied for one month, and the growth rate of the hair grown again by the above-described procedure was calculated. The effect of the sample was evaluated by comparing the hair growth rate before and after using the sample.

3-3. Results From the results in Table 3, a significant increase in hair growth rate was observed for B, C, and D, to which the keratin-associated protein-derived amino acid mixture, which is a hair structure factor, was continuously applied. On the other hand, in the comparative example A, no significant increase in hair growth rate was observed.

毛髪成長速度に対するアミノ酸混合物の作用（ｎ＝４）
Ａ ５０％ エタノール水溶液
Ｂ Ｈｉｇｈ ｓｕｌｆｕｒ ｔｙｐｅのアミノ酸混合物
Ｃ Ｈｉｇｈ ｔｙｒｏｓｉｎｅ／ｇｌｙｃｉｎｅ ｔｙｐｅのアミノ酸混合物
Ｄ Ｈｉｇｈ ｇｌｙｃｉｎｅ／ｓｅｒｉｎｅ／ｃｙｓｔｅｉｎｅ ｔｙｐｅのアミノ酸混合物
（＊Ｂ、Ｃ、Ｄはアミノ酸濃度が３００ｍＭの５０％ エタノール水溶液である。）

Effect of amino acid mixture on hair growth rate (n = 4)
A 50% ethanol aqueous solution B High sulfur type amino acid mixture C High tyrosine / glycine type amino acid mixture D High glycine / serine / cystein type amino acid mixture (* B, C 300% is there.)

As described above, Japanese Patent Application Laid-Open No. 2002-363035 uses three types of amino acid esters, that is, cysteine, arginine, and glutamic acid esters as essential amino acids. However, the amino acid composition of High sulfur type does not include glutamic acid. It has been demonstrated that the hair growth rate is accelerated.
Next, application examples of actual hair-growth cosmetics are given in Examples 2 to 5. However, it is not limited only to these examples. In addition, a compounding quantity is shown by the mass%.

Example 2 Lotion ethyl alcohol 60.0%
Polyoxyethylene hydrogenated castor oil (EO60) 0.5
Dl-α-tocopherol acetate 0.1
β-glycyrrhetinic acid 0.1
1,3-butylene glycol 3.0
High tyrosine / glycine type amino acid mixture 0.01
Purified water total 100

Example 3 Transparent shampoo POE (2) Sodium lauryl ether sulfate aqueous solution 40.0%
Coconut oil fatty acid amidopropyldimethylaminoacetic acid betaine aqueous solution 15.0
POE (5) Lauryl ether 2.0
Palm oil fatty acid diethanolamide 2.0
1,3-butylene glycol 2.0
Edetate disodium 0.2
Methylparaben 0.2
High Glycine / Serine / Cysteine type amino acid mixture 0.1
Purified water total 100

Example 4 Hair cream sorbitan monostearate 2.0%
POE (15) cetyl ether 2.0
Tetraoleic acid POE (30) Sorbit 1.0
Cetanol 0.7
Stearyl alcohol 1.0
Cetyl palmitate 2.0
Hexamethyltetracosane 5.0
Jojoba oil 5.0
Liquid paraffin 25.0
Butylparaben 0.1
1,3-butylene glycol 5.0
Methylparaben 0.1
High sulfur type derived amino acid complex 8.0
Purified water total 100

Example 5 FIG. Cream rinse glyceryl monostearate 1.0%
Stearyltrimethylammonium chloride solution 4.0
Cetanol 2.5
Glyceryl tri-2-ethylhexanoate 2.0
1,3-butylene glycol 5.0
Propylparaben 0.05
Methylparaben 0.2
High Glycine / Serine / Cysteine type amino acid mixture 2.0
Purified water total 100
The above-mentioned 2 to 5 hair-growth cosmetics were prepared by a usual method.

  Each of the hair growth cosmetics of Examples 2 to 5 has good stability, and one month each for 10 male panels for each hair growth cosmetic in the same manner as the evaluation of the human hair growth test of Test Example 3. An actual use test was conducted. Every day, twice a day in the morning and evening, the hair was used in an appropriate amount, the state of the hair before and after use one month later was visually observed, and the hair growth rate was evaluated by comparison. It was confirmed that the hair-growth effect was demonstrated.

It has become possible to provide a hair-growth cosmetic that exhibits an excellent hair-growth effect.

Claims (3)

Among a plurality of amino acids constituting the conserved region of the keratin-associated protein that is a hair structural factor, 8 types of serine, cysteine, alanine, valine, lysine, arginine, glutamine, and proline are used as 7: 11: 1: 1: 1: 1: 1. : A hair-growth cosmetic characterized by containing 0.001 to 10% by weight in a molar ratio of 4: 4. Among the plurality of amino acids constituting the conserved region of the keratin-associated protein that is a hair structure factor, it contains 0.001 to 10% by weight of glycine, tyrosine, and cysteine in a molar ratio of 8: 5: 2. Hair growth cosmetics. Among a plurality of amino acids constituting the conserved region of keratin-associated protein, which is a hair structure factor, 0.004 to 10% by weight of four types of glycine, serine, cysteine, and lysine in a molar ratio of 16: 7: 6: 1 A hair growth cosmetic characterized by the above.