JP4643754B1 - Hair restorer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new use of an extract extracted from an ovarian membrane of a salmon fish.
In the present invention, an extract extracted by treating a ovarian membrane of a salmon fish with a proteolytic enzyme is used as a hair growth agent as a transdermal administration agent.
[Selection] Figure 1

Description

  The present invention relates to a hair restorer comprising an extract extracted from the ovarian membrane of salmon fish.

  Conventionally, a method is known in which fish ovary membrane (egg egg skin) is treated with ozone water in advance, and then myofibrillar protein, which is a constituent protein, is enzymatically decomposed to extract amino acids and peptides (for example, patents). Reference 1).

  It is said that the amino acids and peptides can be used as physiologically active substances or as food fortifiers, and more specifically, have ACE inhibitory activity and act as blood pressure increase inhibitors (hypertensive agents).

  In addition, it is known that an extract obtained by treating the ovarian membrane of a fish of the cod family, cod family, and herring family with a protease that is a proteolytic enzyme is used as a cosmetic preparation (see, for example, Patent Document 2). .

  The extract obtained by treating the ovarian membrane of the codfish family, codfish family, and herring family with a protease activates and suppresses the action of sex hormones and normalizes the balance of sex hormones (sex Hormone modifying action). The cosmetic preparation is said to be effective in improving acne, pimples, rough skin, wrinkles and the like by suppressing the secretion of sebum by the sex hormone modifying action of the extract.

  Furthermore, it is known to use an extract extracted by treating the ovarian membrane of salmon with a proteolytic enzyme as an acceleration pulse wave aging index increasing agent, an IGF-1 level increasing agent, and a rough skin improving agent (patent) Reference 3-5).

JP 2004-73186 A JP 2006-225270 A Japanese Patent No. 3946239 Japanese Patent No. 3948238 Japanese Patent No. 3899116

  However, the extract extracted from the ovarian membrane of fish has no effects other than the high-pressure action of blood pressure, the sex hormone modification action, the acceleration pulse wave aging index raising action, the IGF-1 value raising action, and the rough skin improving action. Development of many more applications is desired.

  In view of such circumstances, an object of the present invention is to provide a new use of an extract extracted from the ovarian membrane of salmon fish.

In order to achieve such an object, the agent of the present invention is characterized by using a component eluted in water as a transdermal administration agent for a hair growth agent by degrading the ovarian membrane of salmon fish with a proteolytic enzyme. .

  The hair repeats hair growth and hair loss at regular intervals. This is called a hair cycle, and is roughly classified into three stages: a growth period, a regression period, and a rest period. Growth period is a period in which hair matrix cells repeat division and hair grows. The regression phase is the period during which the root of the hair that has finished growing moves to the scalp surface. Is the period.

  In a living body, male pattern alopecia, seborrheic alopecia, scoliotic alopecia, alopecia areata and the like may occur, and the amount of hair may decrease. Such a reduction in the amount of hair is caused by, for example, sex hormones, stress, inheritance, diet / lifestyle, and the like, but as a result, the growth period is shortened.

  However, according to the hair restorer of the present invention, since the period of the growth period can be extended, the hair growth effect of alleviating and improving the decrease in the amount of hair associated with hair loss and increasing the amount of healthy hair during the growth period. Obtainable.

  In the present application, the term “hair growth” includes the concept of terms such as “hair growth” and “hair growth”.

The photograph which shows the skin color change of the hair loss area | region of a mouse | mouth when administering the hair restorer of embodiment of this invention.

  The hair growth agent of this embodiment is a transdermal administration agent containing an extract (hereinafter abbreviated as ovarian membrane extract) extracted by treating the ovarian membrane of salmon fish with a proteolytic enzyme.

  Examples of the salmon fish include white salmon, red salmon, silver salmon, rainbow trout, cherry salmon, and salmon. The ovarian membrane of the salmon fish is the fish egg shell of the salmon fish, and can be obtained by washing only the shell after the fish egg is collected from the ovary.

  The ovarian membrane extract is obtained by, for example, a method in which a solution from which protein decomposed by enzymatic treatment of the ovarian membrane separated from fish eggs is precipitated with methanol, and the resulting precipitate is collected by filtration. Obtainable.

  Specifically, in the method, first, salmon fish, for example, Hokkaido ovary membrane of white salmon is used as a raw material, water is added to the ovarian membrane, and the mixture is stirred and mixed. Next, proteolytic enzyme alloase NS (manufactured by Yakult Pharmaceutical Co., Ltd.) is added as a proteolytic enzyme, maintained at pH in the range of 6.5-7, and stirred for 2 hours at a temperature in the range of 43-52 ° C. While reacting. As a result, among the components contained in the ovarian membrane, the components decomposed by the proteolytic enzyme are eluted in water, and an aqueous solution of the components is obtained.

  Next, the proteolytic enzyme contained in the aqueous solution is inactivated. The deactivation can be performed, for example, by heating the aqueous solution at a temperature of 85 ° C. or higher for 10 minutes or more.

  Next, activated carbon is added to the aqueous solution to deodorize, decolorize, and degrease the filtrate. Deodorization, decolorization, and degreasing of the filtrate can be performed by adding activated carbon to the filtrate and heating it at a temperature of 60 ° C. for 30 minutes, for example.

  Next, perlite (manufactured by Toko Perlite Kogyo Co., Ltd.) as a filter aid is added to the filtrate, and the filtrate is filtered by a filter press. The obtained filtrate is reduced to about 1/5 at a temperature of 60 ° C., for example. Concentrate to Thereafter, methanol having a concentration of 99.8% or more of the four times volume ratio is added to the concentrated filtrate, and the precipitate is collected by pressure filtration.

  Next, purified water is added to the obtained precipitate so that the final volume becomes 1 L with respect to 1 kg of the raw material, and an ovarian membrane extract can be obtained.

  The ovarian membrane extract contains 0.04% by mass or more of an active ingredient in terms of nitrogen.

  The hair growth agent of the present invention can be used as a transdermal administration agent, for example, as a coating agent such as cream, emulsion, lotion, hair tonic, spray, pack, solution, ointment, emulsion, suspension. Moreover, the hair restorer of this invention can be mix | blended and used for a shampoo, a conditioner, or a treatment.

  Next, examples of the present invention and comparative examples will be described.

  In this example, C57BL / 6 mice (female, 6-7 weeks old) purchased from Japan SLC Co., Ltd. were prepared as experimental animals, and the experiment was started after acclimatization for 7 days.

  In this experiment, mixed anesthesia prepared with ketamine hydrochloride and xylocaine hydrochloride so as to be 10 mg / kg body weight of each mouse was intramuscularly injected into the back of the mouse. Thereafter, a mixture of pine resin and wax in a weight ratio of 1: 1 was heated and dissolved, and then applied to the back of the mouse. When the mixture solidified, the back of the mouse was depilated by removing the mixture.

  In the mouse, the color of the skin changes depending on the growth stage of the hair, black in the growth period, gray in the regression period, and red in the rest period. Therefore, by observing the color of the mouse skin, it is possible to confirm when the hair is.

  In this example, as described above, the hair cycle can be induced in the growth period by removing the hair of the mouse.

  Next, 0.2 ml of the ovarian membrane extract was applied as a transdermal agent twice a day to the hair loss region of the mouse, and was reared until 25 days after hair removal. Then, record the number of days it takes for the skin color after hair removal to change to black, the number of days it takes for it to change to black, and the number of days it takes for it to change to black after turning black. did. The results are shown in Table 1.

  When the skin color of the mouse hair loss area changed to black, it means that the hair has progressed to the growth phase, and from black to gray means that the hair has progressed from the growth phase to the regression phase. To do.

  Moreover, about the mouse | mouth of a present Example, after the hair removal, the photograph of the hair loss area | region in the 0th day, the 3rd day, the 15th day, and the 25th day was image | photographed. The results are shown in FIG.

[Comparative example]
In this example, a mouse having a hair loss region on the back was prepared in exactly the same manner as in the example. Thereafter, the hair loss region was kept untreated until the 25th day after hair removal. Then, record the number of days it takes for the skin color after hair removal to change to black, the number of days it takes for it to change to black, and the number of days it takes for it to change to black after turning black. did. The results are shown in Table 1.

Moreover, about the mouse | mouth of a present Example, after the hair removal, the photograph of the mouse | mouth back in the 0th day, the 3rd day, the 15th day, and the 25th day was image | photographed. The results are shown in FIG.


A t-test was performed between each example and the comparative example, and when p <0.05, it was determined that there was a statistically significant difference.

  According to FIG. 1 and Table 1, the mice that were transdermally administered with the vaginal ovarian membrane extract of Example 1 had a statistically longer growth period compared to the untreated mice after hair loss of Comparative Example 1. It is clear that it is very significantly longer (p <0.01).

  Here, the terms “hair growth”, “hair growth”, and “hair growth” are generally not clearly defined, but in this application, the term “hair growth”, “hair growth”, and “hair growth” It means to increase the amount of hair.

  According to the hair restorer containing 含有 ovarian membrane extract, as described above, the period of the hair growth period can be lengthened, so the reduction in the amount of hair associated with hair loss is alleviated and improved, and the growth period is healthy. It is clear that the effect of hair growth, hair growth, and hair nourishment that increases the amount of hair can be obtained.

Claims (1)

A hair restorer characterized by being a transdermal administration material containing components eluted in water by degrading the ovarian membrane of salmon fish with a proteolytic enzyme.