JP4590638B2 - 家族性筋萎縮性側索硬化症(fals)発症するトランスジェニックマウス - Google Patents
家族性筋萎縮性側索硬化症(fals)発症するトランスジェニックマウス Download PDFInfo
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- JP4590638B2 JP4590638B2 JP2006077514A JP2006077514A JP4590638B2 JP 4590638 B2 JP4590638 B2 JP 4590638B2 JP 2006077514 A JP2006077514 A JP 2006077514A JP 2006077514 A JP2006077514 A JP 2006077514A JP 4590638 B2 JP4590638 B2 JP 4590638B2
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Description
に関する。
人間の分子遺伝学、2003、第12巻、No.21、第2753頁〜第2764頁(Human Molecular Genetics,2003,Vol.12,No.21 P.2753−P.2764)
Wild human SOD1 amino acid
Ala Thr Lys Ala Val Cys Val Leu Lys Gly Asp Gly Pro Val Gln Gly Ile IleAsn Phe
5 10 15 20
Glu Gln Lys GluSer Asn Gly Pro Val Lys Val Trp GlySer Ile Lys Gly Leu Thr Glu
25 30 35 40
Gly Leu His Gly PheHis Val His Glu Phe Gly Asp Asn Thr Ala Gly Cys Thr Ser Ala
45 50 55 60
Gly Pro His Phe Asn Pro LeuSer Arg Lys His Gly Gly Pro Lys Asp Glu Glu Arg His
65 70 75 80
Val Gly Asp Leu Gly AsnVal Thr Ala Asp Lys Asp Gly Val Ala Asp Val Ser IleGlu
85 90 95 100
Asp Ser Val IleSer Leu Ser Gly Asp His Cys Ile IleGly Arg ThrLeu Val Val His
105 110 115 120
Glu Lys Ala Asp Asp Leu Gly LysGly Gly AsnGlu Glu Ser Thr Lys ThrGly Asn Ala
125 130 135 140
Gly Ser Arg Leu Ala Cys Gly Val Ile GlyIle Ala Gln ***
145 150 155
作製した、2塩基欠失SOD1ゲノム(D)の遺伝子のアミノ酸配列は、下記の通りである。
D human SOD1 amino acid
Ala Thr Lys Ala Val Cys Val Leu Lys Gly Asp Gly Pro Val Gln Gly Ile IleAsn Phe
5 10 15 20
Glu Gln Lys GluSer Asn Gly Pro Val Lys Val Trp GlySer Ile Lys Gly Leu Thr Glu
25 30 35 40
Gly Leu His Gly PheHis Val His Glu Phe Gly Asp Asn Thr Ala Gly Cys Thr Ser Ala
45 50 55 60
Gly Pro His Phe Asn Pro LeuSer Arg Lys His Gly Gly Pro Lys Asp Glu Glu Arg His
65 70 75 80
Val Gly Asp Leu Gly AsnVal Thr Ala Asp Lys Asp Gly Val Ala Asp Val Ser IleGlu
85 90 95 100
Asp Ser Val IleSer Leu Ser Gly Asp His Cys Ile IleGly Arg ThrLeu Val Val His
105 110 115 120
Glu Lys Ala Asp Asp Gly Gln ArgTrp Lys ***
125 130 135
また、2塩基欠失SOD1ゲノム(D)の全塩基配列は、下記の通りである。
D human SOD1
1 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
61 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
121 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
181 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCTGCCA
241 ACCAAATAAG AAACTCTATA CTAAGGACTA AGAAAATTGC AGGGGAAGAA AAGGTAAGTC
301 CCGGGATTGA GGTGTAGCGA CTTTCTATAC CCTCAGAAAA CTAAAAAACA AGACAAAAAA
361 ATGAAAACTA CAAAAGCATC CATCTTGGGG CGTCCCAATT GCTGAGTAAC AAATGAGACG
421 CTGTGGCCAA ACTCAGTCAT AACTAATGAC ATTTCTAGAC AAAGTGACTT CAGATTTTCA
481 AAGCGTACCC TGTTTACATC ATTTTGCCAA TTTCGCGTAC TGCAACCGGC GGGCCACGCC
541 CCCGTGAAAA GAAGGTTGTT TTCTCCACAT TTCGGGGTTC TGGACGTTTC CCGGCTGCGG
601 GGCGGGGGGA GTCTCCGGCG CACGCGGCCC CTTGGCCCCG CCCCCAGTCA TTCCCGGCCA
661 CTCGCGACCC GAGGCTGCCG CAGGGGGCGG GCTGAGCGCG TGCGAGGCGA TTGGTTTGGG
721 GCCAGAGTGG GCGAGGCGCG GAGGTCTGGC CTATAAAGTA GTCGCGGAGA CGGGGTGCTG
781 GTTTGCGTCG TAGTCTCCTG CAGCGTCTGG GGTTTCCGTT GCAGTCCTCG GAACCAGGAC
841 CTCGGCGTGG CCTAGCGAGT TATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGC
901 CCAGTGCAGG GCATCATCAA TTTCGAGCAG AAGGCAAGGG CTGGGACGGA GGCTTGTTTG
961 CGAGGCCGCT CCCACCCGCT CGTCCCCCCG CGCACCTTTG CTAGGAGCGG GTCGCCCGCC
1021 AGGCCTCGGG GCCGCCCTGG TCCAGCGCCC GGTCCCGGCC CGTGCCGCCC GGTCGGTGCC
1081 TTCGCCCCCA GCGGTGCGGT GCCCAAGTGC TGAGTCACCG GGCGGGCCCG GGCGCGGGGC
1141 GTGGGACCGA GGCCGCCGCG GGGCTGGGCC TGCGCGTGGC GGGAGCGCGG GGAGGGATTG
1201 CCGCGGGCCG GGGAGGGGCG GGGGCGGGCG TGCTGCCCTC TGTGGTCCTT GGGCCGCCGC
1261 CGCGGGTCTG TCGTGGTGCC TGGAGCGGCT GTGCTCGTCC CTTGCTTGGC CGTGTTCTCG
1321 TTCCTGAGGG TCCCGCGGAC ACCGAGTGGC GCAGTGCCAG GCCCAGCCCG GGGATGGCGA
1381 CTGCGCCTGG GCCCGCCTGG TGTCTTCGCA TCCCTCTCCG CTTTCCGGCT TCAGCGCTCT
1441 AGGTCAGGGA GTCTTCGCTT TTGTACAGCT CTAAGGCTAG GAATGGTTTT TATATTTTTA
1501 AAAGGCTTTG GAAAACAAAA ATACGCAACA GAGACCGTTT GTGTGACACT TTGCAGGGAA
1561 GTTTGCTGGC CTCTGTTCTA GGTCATGATT GGGCTGCAAG GGCAGAGAAG GTAGCCTTGA
1621 ACAGAGGTCC TTTTCCTCCT CCTAAGCTCC GGGAGCCAGA GGTTTAACTG ACCCTTTTGG
1681 GGATTTTTGA GGGCAGTGAT CTTAACTTTG GGTGCACAGT TAGCTTATTT GAAGATCTTA
1741 CTAAAAATAC ACCAGAGCCC AACCTCCGAC CAATTACATC AAAACCTGTC CTAGTGCAGG
1801 GTGAGTATTG CTGTTTTTTG AAAGTTTCCA AAAGTGATTT TGATGTGCAC CTACGATTGA
1861 GAACTGTCGT TTGAGGACAG TGGGTGGAGT TTCGTATTTG GAAATTAGAA GACCTGGAGT
1921 TTCCATTACA CCGAATTGGC ACTTAATAAC TGTTGTCGGA GCATTTCTTA AGCCACATTT
1981 TCGTAAAGTG GCTTTAAAAT TGCTCTGCCA GTAGGCAGGT TGCTAAGATG GTCAGAGACA
2041 AACTTCTGAA CGACTCTTGT AAAATATACA GAAATATTTT CAGAACTTTT ATCAGTAAAA
2101 TTACAAAACG TGTTGCAAGG AAGGTGCTTG TGATAACACT GTCCCCAGAA CCTTAGTGAA
2161 GTTACCAACT GGTGGAAAAT TTTCTCTTGC ACTCGGCTTA AAAATCATGA GGGAATATTT
2221 ACTATACGAA TGAGATTCAG TCTTTAAAGG GGTTTACAGA AACGTGAGAG GACAGGAACA
2281 GTTAGTCTGT GTAAATGTCT GAAATATATG TGAGGGAGAT AATGAGTTTA GCCTTTTTCT
2341 TTAATAGGTC TCCAGATTTT CTGGAAAAGG TTCTTTGGCA TTTGACTCCA TTTTGCTGTT
2401 TCATTTGTCA GACTTCTTTT TGTCCCTCTT TACTTCTCCC CACATAATTC ACCAGTACTA
2461 GTGTTTTGTT TTTCAGACCA AGTCTCGCTC TGTCGCCCAG GCTGGAGTGC AGTGGCGCGA
2521 TCTCAGCTCA CTGCAACCTC CGCCTCCCAG GTTCAAGCAA TTCTCCTGCC TCAGCCTCCC
2581 GGGTAGCTGG GACTACAGGC GCGCGCCGCC ACGCCTGGCT AATTTTTTAT ATTTTAGTAG
2641 AGACGGCGTT TCACCATGTT GGCCAGGATG GTCTCGATCT GTTGACGTCG TGATCCACCC
2701 GCCTCGGCCT CCCAAAGTGC TGGGATTACA GGCGTGAGCC ACCCCGCCCG GCCACCAGTG
2761 CTATTCTTAA GACGCCTCTG AGGAATCCCT TCTCCCTGGC CATTGAGAAT CCATGCATGA
2821 ACCCAGGTTT TCCACCTTCC CTGAGCAGCT TGCATAGTTC CTTCTTTTAA GCGCCTGACT
2881 TCGTTTTGTT TGGTGCCCGT TGTACCTGAG AATGAGCCTT GGATAGTGGA GCATTCCAGC
2941 TTTCCAGATA TGCAGAGATA ATACATTGGC TATCAGCTAC TTGGCTTGGC CTATTCCGTG
3001 TTTAAAATCT TGGACTCTTT GCTAGTTTTT ACAGATCAGA ATTTTTCACG TATTAATCCA
3061 GTTTTCCTAG CTTCTCTTGA AGAATTTTTG GAGATCTCTT CATACTGAGC CTTCATTAGC
3121 CCAGGACAGT ACTGCTGTAG CAGTTCATAT ATTTTTTCGC TTCCCAGGCC TGTGTTATTC
3181 ACTTAAGTTC ATAGCCTGGT CCCTGCAGGG TTGTACCCGA GCACAGCTAC TTAGATGTCC
3241 TGAATGTATT ACCGGTTAAA TGGAGGTTTC AAAGAACCTG CTGTTTTTGG CCCTGTGCTC
3301 TTGATAACAG AGTGTTTGAG GGACAACTTT CACATTTGAG TTTTTCCAAA ATTAAAGGTT
3361 GTAGAAGAGT CACAGTATCT ATTGTCAAAA AGAAAAGAAT TTAAAAAGGC AGCAATTGCC
3421 AGGATACTTC ATTTGAGCAA TGATATTTTC CAGTGGAAAG TCACATCTTA AGGGTTAATG
3481 CCCCTTAACT GTTGGCCGTA TTTGAAAACA AACCAAGCTA AAAACAAGAG ACACTGACAT
3541 GTTGTATGAC GGTGTGGTGT GGATGTTGTG TTTATTTTAG TCCTGAGATC TAGTTGTAAC
3601 TTCCTTGATT TCTGTATGTA GCCACGGAGC ACCATTACCT GTCACCATTA CCTGAATGGC
3661 TATACTGCTT GCTTTCATTT TGGTAGAGTG GAAAGGTTAC CTAGGTTTCA GTGCTTGAAA
3721 AGATTTCAGA AAGCAGTAGT ACGTCTGGTT AGACTAGAAT CAGTCCTCTC CTGGGGGCAG
3781 TGGAATATAA TATTTTCTGA CTGCTAATTA AAAATACCTG TGATAGCCGG GCGTGGTGGC
3841 TTACGCCTGT AATCCCAGCA CTTTGGGAGG CCGAGACGGG TGGATCACGA GGTCAGCAGA
3901 TGGAGACCAT CCTGGCTAAC ACGGTGAAAC CCCGTCTCTA CTAAAAATGC AAAAAAATTA
3961 GCCGGGTGTG GTGGTGGGCG CCTGTAGTCC CAGCTACTCA GGAGGCTGAG GCAGGAGAAT
4021 GGCATGAACC TGGGAGGCGG AGCTTGCAGT GAGCCGAGAT CATGTCACTG CACTCCAGCC
4081 TGGGCGACAG AGCGAGACTC GTCTCAAAAA AAAAAAGAAA AAAACTTATG ATGGACACTT
4141 AAAAACACTC ACTGAGTGGG GAGTGGAGAG CAGGGGTCCC AGGGTAGCCT GTTGGACATT
4201 TCCAGGGCGA CTTTTTCTTT TTTTTTTTTT AAAGTCAAGT GAGTATGCCA TATGGAAAAG
4261 GGTGTGCGTG GAGAAAAAGC AAGGGGCTCC AGAGTGTAGG ATGAGACATA CACCTTTTGG
4321 GTTAAAAAGG CTGAGGCAGG AGAATGGCGT GAACCCGGGA GGCGGAGCTT GCAGTGAGCT
4381 GAGATCATGC CACTGCACTC CAGCCTGGGC GACAGAGCGA GACTCTTGTC TCAAAATAAA
4441 AAACGTTTAC ATGTACATGT ATATTCAACA TGTACAAATA TAACCTATTC AAAAGTATTT
4501 ACTACATAAA TAGGTACTTA CATTACCTAT TTACTGTAAT AGTCAAAGCC TATGAAGTAT
4561 CTAACACTGA TGTGTAGGTA CTCACTTTGC TTGCCACTCT ATTAGGTGCT TTTTATGTTA
4621 TTTAATCATG AAGCCTGGCC ACAGGGTGCT TGTGCATTGA GTGTGGGAAC AAGATTACCA
4681 TCTCCCTTTT GAGGACACAG GCCTAGAGCA GTTAAGCAGC TTGCTGGAGG TTCACTGGCT
4741 AGAAAGTGGT CAGCCTGGGA TTTGGACACA GATTTTTCCA CTCCCAAGTC TGGCTGCTTT
4801 TTACTTCACT GTGAGGGGTA AAGGTAAATC AGCTGTTTTC TTTGTTCAGA AACTCTCTCC
4861 AACTTTGCAC TTTTCTTAAA GGAAAGTAAT GGACCAGTGA AGGTGTGGGG AAGCATTAAA
4921 GGACTGACTG AAGGCCTGCA TGGATTCCAT GTTCATGAGT TTGGAGATAA TACAGCAGGT
4981 GGGTGTTGTG CTGTGCTGGT GACCCATACT TGTTCACCCT AGTTAGATAA ACAGTAGAGT
5041 AGCCCCTAAA CGTTAAAACC CCTCAACTTG TTTTTGTTTT TGAGAAAGGG TCTTGCTCTG
5101 TCGCTCAGGC TGGAGTGCAG TGGCGCTGTG CGATCATGGC TGACCTTAGC CTTGACCTCC
5161 CAGGCTCCAT TGATCCTCAT GCCTTGGCCC GTAGCTGGGA CTACAGGTAC ACACCACCAC
5221 GCCTGGCTAA TTTTTGTATT TTTTTCTAGA GGTGGGGTTT CATCATGTTG CCCAGGCTGG
5281 TCTTGAACTG CTGGGCTCAA GTGGTCTATC CTCCTCGACC TCCCAAAGTG CTGGGATTAC
5341 ATGTGTGAGC CACTGTGCCT GGGAAAACCC TCAACTTTTC TTTTAAAAAA GAGGTCAACT
5401 TTATTGTATA TAAGCACTGT GCTAAAATTG CAGGAACTGG GACCATATCC TGATTTTTGT
5461 AATAATGCCA GCAGAGTACA CACAAGAAAA GTAACTGCAC TAGATTGTGA AGACTGGGGT
5521 GGACCTGCTT CTGAAGGTCC AGTGCCCTTT GTCTTAAGAT TTGGTGTAGT GTGTCTTTAG
5581 AAACCAAAAA AAGAGAAGAA GATCAACCTT AAGATTAGCC ACAAAACTGG GCTTTGATAC
5641 CTAGGTGTGG AAAAGAAAGG GAAAGAGTTG ATGTTTTGTC TTACAGCATC ATTGTAGAAG
5701 AGGGTGTTTT TTTGTTTGTT TGTTTTTTGA GACGGAGTCT TACTCTGTGG CCCAGGCTGG
5761 AGTGCAGTGG CGCGATCTCG GCTCACTGCA AGCTCCGCCT CCCGGGTTCA TGCCATTCTC
5821 CTGCCTCAGC CCCCTGAGTA GCTGGGACTA CAGGTGCCCG CCACCCCGCC TGGCTAATTT
5881 TTTGTATTTT TAGTAGAGAC GGGGTTTCAC TGTGTTAGCC AAGATGGTCT CTCTCCTGAC
5941 CTCGTGATCC GCCTGTCTCA GCCTCCCAAA GTGCTGGGAT TACAGGCATG AGCCACCGCA
6001 CCCAGCCAGA AGAGGGTGTT TTTTAAAGAA GGCAAATAGG AAATAAAAAC TTGGGCTCTT
6061 AACTTTTGTA ATGATCCCAG GTGTTTGAGC TGGGGGTTGA GGGTGGGTGC CTCGAGCAAA
6121 GGGGCTGCAT TTATTTGCAT AATGCCATGT AAGAGTAGCT CTACACCCCA AACACAGGCT
6181 TCTTAGTGGG ACCAAAGTAT GATACAAACT GAAGATGGAA TGCAGAGGAT TATTGGTACT
6241 TTGGAATATG CTTAAAAAAA ATTTTTTTAA AGTATTTTTA AAAAATCAGG CAACCCCTGA
6301 ACCAGAGTAG GTTCAGAGAA ACTGCCAAAT TTTATTTTCT TAATTTGGGA TTGGAAGCAA
6361 GTTAACAGAA GTTTATGAGT TAAGTTGCAT TTAGTGATCT TTTGCCATAT TTGAGTAATA
6421 ATCTGATTTT TTTGTTTATA GATTTCTTCT TAAATTAACT TTATTCATCT TGCTAATTTA
6481 GTTTCAAATA GTGATTTGTA ATGATCAGAT TTGATCCATT TCTGTAATTG CTGAAATTCC
6541 CCCGAGTTGC TTTTTGGCTT TACCGCCTCT GGTCTGGGAG GTGATTGCTC TGCTGCTTCC
6601 TGTAACTTGC CTGCCTTTCT CCCTGTGTGG GACTCCTGCG GGTGAGAGCG TGGCTGAAGA
6661 CAGCCGTGTT ATGAAAGGGC CTCCTGTGCT GTCGAGGTTG TGCTCTGTGA ATGTCATCCC
6721 CTGGTGCACA GCAGCACCTT CTACACAGGA TACAGTTGGA ATGCCGCCCC CTCGAGTTGT
6781 GTAAGGCAGC AGCCTTGGCC CTTGCACATA AGATGCTGTT GAATATTCTG CCTGCACCAA
6841 GTAAAGGGCA CAGATAGAAC TGCTTGGCAT ATGTTGCTGG GGAGATGAGT TTTTTGTAAA
6901 GTATACTACG TTCTTAAGAA TTTGGATCAT AACCATGGGA TTTTAATAAT AGAAAAACTG
6961 TTGAAGATCA GTCTGGTCCC TTATTTTTAC AGTGAAGAAG CCAAAGCCCA GAGAAGGGTG
7021 TTAACTTTAC AAGTGTCAGA CAGTAGTTAG AACTTGGTGG GGTTTTTTTT TTTTTTTTTT
7081 TGAGATGGAG TCTTGCTCTG TTGCCCAGGC TGGAGTGCAG TGGTGCGATC TCAGCTCACT
7141 GCAACCTCTG CCTCCCAGGT TCAAGCGATT CTCCTGCCTC AGCCTACTAA GTAGCTGGGA
7201 CTATAGGTGC GCACCACCAC GCCTAGCTAA TTTTTGTATT TTTTCAGTAG AGACAGGGTT
7261 TTGCTATGCT GGCCAGGCTG GTCTCAAACT CCTGACCTCA GATGATCCAG CCACCTCAGC
7321 TTCCCAAAGT GCTGGGGTTC CAGGTGTTAG CCACCATGCC TGGCCATAGA CTTGTTTCTG
7381 TTCCCTTCTC ACTGTGGCTG TACCAAGGTG TTGCTTATCC CAGAAGTCGT GATGCAGGTC
7441 AGCACTTTCT CCATGGGAAG TTTTAGCAGT GTTTCTTTTT AGAATGTATT TGGGAACTTT
7501 AATTCATAAT TTAGCTTTTT TTTCTTCTTC TTATAAATAG GCTGTACCAG TGCAGGTCCT
7561 CACTTTAATC CTCTATCCAG AAAACACGGT GGGCCAAAGG ATGAAGAGAG GTAACAAGAT
7621 GCTTAACTCT TGTAATAATG GCGATAGCTT TCTGGAGTTC ATATGGTATA CTACTTGTAA
7681 ATATGTGCTA AGATAATTCC GTGTTTCCCC CACCTTTGCT TTTGAACTTG CTGACTCATC
7741 TAAACCCCTG CTCCCAAATG CTGGAATGCT TTTACTTCCT GGGCTTAAAG GAATTGACAA
7801 ATGGGGACAC TTAAAACGAT TTGGTTTTGT AGCATTTATT GAATATAGAA CTAATACAAG
7861 TGCCAAAGGG GAACTAATAC AGGAAATGTC ATGAACAGTA CTGTCAACCA CTAGCAAAAT
7921 CAATCATCAT TGTGAAACAT AGGAAGCTTC TGTAGATAAA AAAAAAAATT GATACTGAAA
7981 ACTAGTCGAG ACTCCATTTA TATGTGTATG TTTTCTGAAA GCCTTTCAGA AAAATATTAA
8041 ATTTAAGGAC AAGATTTTTA TATCAGAGGC CTTGGGACAT AGCTTTGTTA GCTATGCCAG
8101 TAATTAACAG GCATAACTCA GTAACTGAGA GTTTACCCTT TGGTACTTCT GAAATCAGGT
8161 GCAGCCCCAT CTTTCTTCCC AGAGCATTAG TGTGTAGACG TGAAGCCTTG TTTGAAGAGC
8221 TGTATTTAGA ATGCCTAGCT ACTTGTTTGC AAATTTGTGT CTACTCAGTC AAGTTTTAAT
8281 TTAGCTCATG AACTACCTTG ATGTTTAGTG GCATCAGCCC TAATCCATCT GATGCTTTTT
8341 CATTATTAGG CATGTTGGAG ACTTGGGCAA TGTGACTGCT GACAAAGATG GTGTGGCCGA
8401 TGTGTCTATT GAAGATTCTG TGATCTCACT CTCAGGAGAC CATTGCATCA TTGGCCGCAC
8461 ACTGGTGGTA AGTTTTCATA AAAGGATATG CATAAAACTT CTTCTAACAT ACAGTCATGT
8521 ATCTTTTCAC TTTGATTGTT AGTCGCGGTT TCTAAAGATC CAGATAAACT GTACTTGCAG
8581 TTCAAATTAG GAAAAGCAAT TTTATTGGAC AATTACGGTG AAAATGAATT ATTTTATCTA
8641 GGTCAGTTAA GAACACTGTT CTGCTAAGAT GCAGTAAAAA GCAGGTTACA TTTGACCATA
8701 TTAGATCTGA GTTTGGAAAA CAGAAGTAGT CTTTAGTTTT AAAATGGCCA GATTTTCTTG
8761 CCAGGATTGG GTTTCTCACT TGTTAAACAG AACATTTTGT TAAGTTTAAA ACCTGGGATG
8821 GACTTAAGTA TTCATGTTCA TTCATGTTCA TTCAGGACTG CAGGTTATCA TGACTTGTTT
8881 AACTTGTGGG AAGCTGTTGT CCCAAGTTAT CCTGGGGAAC TGCATCTGGT TCTTGCAAAA
8941 CACCAAGTAG ACAGGCTCTC TTTTACCTCC CCTTGAGGGC ATTAACATTC AGTAGTCACT
9001 TCCATTCAGT TAACCCTTTA TTTTTATGGT TTTTCTTGAG CCATAGTTGT AAAGCAGAAA
9061 AATCATTTAT AAAGGTTTGT TGAACAAAAT TCAAAATACT GTTGCTTAAA GTATTAAGAT
9121 TTTTTAGGAT TATACCTTAC TTATAGGCCC GTCATTCATT TGGCATGAAA TTTTGAGTTT
9181 TATTCACTTT CACTTTCCTT TTTTTCCAAA GCAATTAAAA AAACTGCCAA AGTAAGAGTG
9241 ACTGCGGAAC TAAGGTTACT GTAACTTACC ATGGAGGATT AAGGGTAGCG TGTGGTGGTC
9301 TACAACATAG TTATTTGGGT TTTAGTATTT CATTTAGACA GCAACACTTA CCTAATGTTT
9361 AAAGGTAATG TCTTTGCAAC ACCAAGAAAA AGCTTTGAGT AGTAGTTTCT ACTTTTAAAC
9421 TACTAAATAT TAGTATATCT CTCTACTAGG ATTAATGTTA TTTTTCTAAT ATTATGAGGT
9481 TCTTAAACAT CTTTTGGGTA TTGTTGGGAG GAGGTAGTGA TTACTTGACA GCCCAAAGTT
9541 ATCTTCTTAA AATTTTTTAC AGGTCCATGA AAAAGCAGAT GACGGGCAAA GGTGGAAATG
9601 AAGAAAGTAC AAAGACAGGA AACGCTGGAA GTCGTTTGGC TTGTGGTGTA ATTGGGATCG
9661 CCCAATAAAC ATTCCCTTGG ATGTAGTCTG AGGCCCCTTA ACTCATCTGT TATCCTGCTA
9721 GCTGTAGAAA TGTATCCTGA TAAACATTAA ACACTGTAAT CTTAAAAGTG TAATTGTGTG
9781 ACTTTTTCAG AGTTGCTTTA AAGTACCTGT AGTGAGAAAC TGATTTATGA TCACTTGGAA
9841 GATTTGTATA GTTTTATAAA ACTCAGTTAA AATGTCTGTT TCAATGACCT GTATTTTGCC
9901 AGACTTAAAT CACAGATGGG TATTAAACTT GTCAGAATTT CTTTGTCATT CAAGCCTGTG
9961 AATAAAAACC CTGTATGGCA CTTATTATGA GGCTATTAAA AGAATCCAAA TTCAAACTAA
10021 ATTAGCTCTG ATACTTATTT ATATAAACAG CTTCAGTGGA ACAGATTTAG TAATACTAAC
10081 AGTGATAGCA TTTTATTTTG AAAGTGTTTT GAGACCATCA AAATGCATAC TTTAAAACAG
10141 CAGGTCTTTT AGCTAAAACT AACACAACTC TGCTTAGACA AATAGGCTGT CCTTTGAAAG
10201 CTTTAGGGAA ATGTTCCTGC TTAGTCATTT TAGCATTTTG ATTCATAAAG TACCTCCTCA
10261 TTTTAAAAAG ACATTATGAT GTAAGAGAGC CATTTGATAA CTTTTTAGTG AGCTTTGAAA
10321 GGCAAGTTAC AGCCTCAGCT AGCTAGTAAG ATTATCTACC TGCCAGAATG GCACAAATTC
10381 TACATTCAAG GGTAGACGCT GGCACAACCT ACTTACAGAT TAGCCCTTTA AAGCAATCTG
10441 TAGCATTAGA AGATGGAACC AAGGAAATGT TTGACTGTGG GTTCTGGCTG TTGAGAAATA
10501 ATTTACACAC CGAATTAGTG AAATGAGTCA CTTTCTCTTA ATGTATTTAT GTACCTGAGA
10561 GAATGCTTTT CAATGTTAAC CTAACTCAGG TTTGACTAAA TTATTCAATT GGAAATTGTA
10621 GAATATTATT TCTGATAAAC CAGAAATAAG TGAAATGCTG TTTGTTCATA AATATGTACT
10681 TTATCAAATG TAGGAGAGAT CATTTAGGAG AGGAAAAGCT AAATTGGAAG ACAAATCTGT
10741 AGTGTTTCCA AAGTTTTAAA ATTATGGTAA ACAACAGTAT GTTCACAGTA AGTGGTTAAA
10801 ACAACCATTC TTTAAATCTC AGTAGAGAAT TTTTAAAAAG CAGTATTTAA CACATTTCCC
10861 TAATGTAGTT TGTTGCCTAT GTGGAATAAC TCAATTAGAG ACTCACTTAT GCCTTTTGAA
10921 ACTTCAAATA TAATTACACT ACCAGTTTTT ACATGTGCAT ATAGGATGGT CCCAATACTT
10981 TAAATTGGAA ATACAGGCTG TAAGTCCTTC AAGTCTGGAT GTTGGGTAAT CACGTTTTCT
11041 TCCAGAAGCC ATTTGTTAGG ACTTTAAAAC TTCTCAGTGG GCCAGTGTAA AATTAAGGAC
11101 AAGTTTTATA ATTTAAATTT ACAGATAAAT ATAAAACAAT TTTCTCTCAT TTTCTAAAGG
11161 CAGGAATATA AGGACATTGC CCTAGATATA TTCTCCCCAT CAAACCAAAG GTCTTGCTGC
11221 TGAGTATTAT TTAAAAACCA GAGTAATAAA GCAGCTTCGC CTGAGGATGA AGGCAAAGAC
11281 AAAGTTAACT GCTCAGCATA CTGAAACCAG GAGTTTGAAA AAGTAATTCA AAACAACTTC
11341 AAGGCCATAA ATACTTGGAT AGTGTGACAT CAGGTCTTGG CACTGGATTT CCTGCAATTT
11401 CAGATGAAGG CTTGACTTGG CTTTAGATGT TTTCATGTGA TAATTCTTGG ATAACCTACA
11461 ACGATTTTTC CTATTTAACC CACCAACTTT AAGCAAAAGA AAGAACGTAT ATGTGGGGGA
11521 AAAGTTCGTT TACCTCTGCC CCGCAGGGGA ATTAAAACGC TGGTGAAGAA AGGCAACAGG
11581 CAAGGCTTAT ATAAGGAAAC AGTAGTAGGC TGCACTGAAG TCCTCAAACA CAACAAAAAG
11641 CTCTTAGGTT GCAAATGTTA ACCTTGATTC TTTTACCCTT TTGAAAAATT CAATGGGATG
11701 GTTGGAAAAA AAAAAAACCA CAGACACACA CACACACACA CGCAACCTTC TAACGTAATA
11761 CCCAGGCAGT CCGATAATTT ATAGTACAAC GTGTAACACT GGGATCCTCT AGAGTCGACC
11821 TGCAGGCATG CAAGCTTGGC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT
11881 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC
11941 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGC
//
また、作製した2塩基欠失SOD1ゲノム(D)蛋白のC末端に、フラグ(FLAG)タグ(DYKDDDDK)を付加したコンストラクト(DF)のアミノ酸配列は、下記の通りである。
DF human SOD1 amino acid
Ala Thr Lys Ala Val Cys Val Leu Lys Gly Asp Gly Pro Val Gln Gly Ile IleAsn Phe
5 10 15 20
Glu Gln Lys GluSer Asn Gly Pro Val Lys Val Trp GlySer Ile Lys Gly Leu Thr Glu
25 30 35 40
Gly Leu His Gly PheHis Val His Glu Phe Gly Asp Asn Thr Ala Gly Cys Thr Ser Ala
45 50 55 60
Gly Pro His Phe Asn Pro LeuSer Arg Lys His Gly Gly Pro Lys Asp Glu Glu Arg His
65 70 75 80
Val Gly Asp Leu Gly AsnVal Thr Ala Asp Lys Asp Gly Val Ala Asp Val Ser IleGlu
85 90 95 100
Asp Ser Val IleSer Leu Ser Gly Asp His Cys Ile IleGly Arg ThrLeu Val Val His
105 110 115 120
Glu Lys Ala Asp Asp Gly Gln ArgTrp Lys Asp Tyr Lys Asp Asp Asp Asp Lys ***
125 130 135 140
また、作製した2塩基欠失SOD1ゲノム(D)蛋白のC末端に、フラグ(FLAG)タグ(DYKDDDDK)を付加したコンストラクト(DF)の塩基配列は、下記の通りである。
DF human SOD1
1 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
61 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
121 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
181 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCTGCCA
241 ACCAAATAAG AAACTCTATA CTAAGGACTA AGAAAATTGC AGGGGAAGAA AAGGTAAGTC
301 CCGGGATTGA GGTGTAGCGA CTTTCTATAC CCTCAGAAAA CTAAAAAACA AGACAAAAAA
361 ATGAAAACTA CAAAAGCATC CATCTTGGGG CGTCCCAATT GCTGAGTAAC AAATGAGACG
421 CTGTGGCCAA ACTCAGTCAT AACTAATGAC ATTTCTAGAC AAAGTGACTT CAGATTTTCA
481 AAGCGTACCC TGTTTACATC ATTTTGCCAA TTTCGCGTAC TGCAACCGGC GGGCCACGCC
541 CCCGTGAAAA GAAGGTTGTT TTCTCCACAT TTCGGGGTTC TGGACGTTTC CCGGCTGCGG
601 GGCGGGGGGA GTCTCCGGCG CACGCGGCCC CTTGGCCCCG CCCCCAGTCA TTCCCGGCCA
661 CTCGCGACCC GAGGCTGCCG CAGGGGGCGG GCTGAGCGCG TGCGAGGCGA TTGGTTTGGG
721 GCCAGAGTGG GCGAGGCGCG GAGGTCTGGC CTATAAAGTA GTCGCGGAGA CGGGGTGCTG
781 GTTTGCGTCG TAGTCTCCTG CAGCGTCTGG GGTTTCCGTT GCAGTCCTCG GAACCAGGAC
841 CTCGGCGTGG CCTAGCGAGT TATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGC
901 CCAGTGCAGG GCATCATCAA TTTCGAGCAG AAGGCAAGGG CTGGGACGGA GGCTTGTTTG
961 CGAGGCCGCT CCCACCCGCT CGTCCCCCCG CGCACCTTTG CTAGGAGCGG GTCGCCCGCC
1021 AGGCCTCGGG GCCGCCCTGG TCCAGCGCCC GGTCCCGGCC CGTGCCGCCC GGTCGGTGCC
1081 TTCGCCCCCA GCGGTGCGGT GCCCAAGTGC TGAGTCACCG GGCGGGCCCG GGCGCGGGGC
1141 GTGGGACCGA GGCCGCCGCG GGGCTGGGCC TGCGCGTGGC GGGAGCGCGG GGAGGGATTG
1201 CCGCGGGCCG GGGAGGGGCG GGGGCGGGCG TGCTGCCCTC TGTGGTCCTT GGGCCGCCGC
1261 CGCGGGTCTG TCGTGGTGCC TGGAGCGGCT GTGCTCGTCC CTTGCTTGGC CGTGTTCTCG
1321 TTCCTGAGGG TCCCGCGGAC ACCGAGTGGC GCAGTGCCAG GCCCAGCCCG GGGATGGCGA
1381 CTGCGCCTGG GCCCGCCTGG TGTCTTCGCA TCCCTCTCCG CTTTCCGGCT TCAGCGCTCT
1441 AGGTCAGGGA GTCTTCGCTT TTGTACAGCT CTAAGGCTAG GAATGGTTTT TATATTTTTA
1501 AAAGGCTTTG GAAAACAAAA ATACGCAACA GAGACCGTTT GTGTGACACT TTGCAGGGAA
1561 GTTTGCTGGC CTCTGTTCTA GGTCATGATT GGGCTGCAAG GGCAGAGAAG GTAGCCTTGA
1621 ACAGAGGTCC TTTTCCTCCT CCTAAGCTCC GGGAGCCAGA GGTTTAACTG ACCCTTTTGG
1681 GGATTTTTGA GGGCAGTGAT CTTAACTTTG GGTGCACAGT TAGCTTATTT GAAGATCTTA
1741 CTAAAAATAC ACCAGAGCCC AACCTCCGAC CAATTACATC AAAACCTGTC CTAGTGCAGG
1801 GTGAGTATTG CTGTTTTTTG AAAGTTTCCA AAAGTGATTT TGATGTGCAC CTACGATTGA
1861 GAACTGTCGT TTGAGGACAG TGGGTGGAGT TTCGTATTTG GAAATTAGAA GACCTGGAGT
1921 TTCCATTACA CCGAATTGGC ACTTAATAAC TGTTGTCGGA GCATTTCTTA AGCCACATTT
1981 TCGTAAAGTG GCTTTAAAAT TGCTCTGCCA GTAGGCAGGT TGCTAAGATG GTCAGAGACA
2041 AACTTCTGAA CGACTCTTGT AAAATATACA GAAATATTTT CAGAACTTTT ATCAGTAAAA
2101 TTACAAAACG TGTTGCAAGG AAGGTGCTTG TGATAACACT GTCCCCAGAA CCTTAGTGAA
2161 GTTACCAACT GGTGGAAAAT TTTCTCTTGC ACTCGGCTTA AAAATCATGA GGGAATATTT
2221 ACTATACGAA TGAGATTCAG TCTTTAAAGG GGTTTACAGA AACGTGAGAG GACAGGAACA
2281 GTTAGTCTGT GTAAATGTCT GAAATATATG TGAGGGAGAT AATGAGTTTA GCCTTTTTCT
2341 TTAATAGGTC TCCAGATTTT CTGGAAAAGG TTCTTTGGCA TTTGACTCCA TTTTGCTGTT
2401 TCATTTGTCA GACTTCTTTT TGTCCCTCTT TACTTCTCCC CACATAATTC ACCAGTACTA
2461 GTGTTTTGTT TTTCAGACCA AGTCTCGCTC TGTCGCCCAG GCTGGAGTGC AGTGGCGCGA
2521 TCTCAGCTCA CTGCAACCTC CGCCTCCCAG GTTCAAGCAA TTCTCCTGCC TCAGCCTCCC
2581 GGGTAGCTGG GACTACAGGC GCGCGCCGCC ACGCCTGGCT AATTTTTTAT ATTTTAGTAG
2641 AGACGGCGTT TCACCATGTT GGCCAGGATG GTCTCGATCT GTTGACGTCG TGATCCACCC
2701 GCCTCGGCCT CCCAAAGTGC TGGGATTACA GGCGTGAGCC ACCCCGCCCG GCCACCAGTG
2761 CTATTCTTAA GACGCCTCTG AGGAATCCCT TCTCCCTGGC CATTGAGAAT CCATGCATGA
2821 ACCCAGGTTT TCCACCTTCC CTGAGCAGCT TGCATAGTTC CTTCTTTTAA GCGCCTGACT
2881 TCGTTTTGTT TGGTGCCCGT TGTACCTGAG AATGAGCCTT GGATAGTGGA GCATTCCAGC
2941 TTTCCAGATA TGCAGAGATA ATACATTGGC TATCAGCTAC TTGGCTTGGC CTATTCCGTG
3001 TTTAAAATCT TGGACTCTTT GCTAGTTTTT ACAGATCAGA ATTTTTCACG TATTAATCCA
3061 GTTTTCCTAG CTTCTCTTGA AGAATTTTTG GAGATCTCTT CATACTGAGC CTTCATTAGC
3121 CCAGGACAGT ACTGCTGTAG CAGTTCATAT ATTTTTTCGC TTCCCAGGCC TGTGTTATTC
3181 ACTTAAGTTC ATAGCCTGGT CCCTGCAGGG TTGTACCCGA GCACAGCTAC TTAGATGTCC
3241 TGAATGTATT ACCGGTTAAA TGGAGGTTTC AAAGAACCTG CTGTTTTTGG CCCTGTGCTC
3301 TTGATAACAG AGTGTTTGAG GGACAACTTT CACATTTGAG TTTTTCCAAA ATTAAAGGTT
3361 GTAGAAGAGT CACAGTATCT ATTGTCAAAA AGAAAAGAAT TTAAAAAGGC AGCAATTGCC
3421 AGGATACTTC ATTTGAGCAA TGATATTTTC CAGTGGAAAG TCACATCTTA AGGGTTAATG
3481 CCCCTTAACT GTTGGCCGTA TTTGAAAACA AACCAAGCTA AAAACAAGAG ACACTGACAT
3541 GTTGTATGAC GGTGTGGTGT GGATGTTGTG TTTATTTTAG TCCTGAGATC TAGTTGTAAC
3601 TTCCTTGATT TCTGTATGTA GCCACGGAGC ACCATTACCT GTCACCATTA CCTGAATGGC
3661 TATACTGCTT GCTTTCATTT TGGTAGAGTG GAAAGGTTAC CTAGGTTTCA GTGCTTGAAA
3721 AGATTTCAGA AAGCAGTAGT ACGTCTGGTT AGACTAGAAT CAGTCCTCTC CTGGGGGCAG
3781 TGGAATATAA TATTTTCTGA CTGCTAATTA AAAATACCTG TGATAGCCGG GCGTGGTGGC
3841 TTACGCCTGT AATCCCAGCA CTTTGGGAGG CCGAGACGGG TGGATCACGA GGTCAGCAGA
3901 TGGAGACCAT CCTGGCTAAC ACGGTGAAAC CCCGTCTCTA CTAAAAATGC AAAAAAATTA
3961 GCCGGGTGTG GTGGTGGGCG CCTGTAGTCC CAGCTACTCA GGAGGCTGAG GCAGGAGAAT
4021 GGCATGAACC TGGGAGGCGG AGCTTGCAGT GAGCCGAGAT CATGTCACTG CACTCCAGCC
4081 TGGGCGACAG AGCGAGACTC GTCTCAAAAA AAAAAAGAAA AAAACTTATG ATGGACACTT
4141 AAAAACACTC ACTGAGTGGG GAGTGGAGAG CAGGGGTCCC AGGGTAGCCT GTTGGACATT
4201 TCCAGGGCGA CTTTTTCTTT TTTTTTTTTT AAAGTCAAGT GAGTATGCCA TATGGAAAAG
4261 GGTGTGCGTG GAGAAAAAGC AAGGGGCTCC AGAGTGTAGG ATGAGACATA CACCTTTTGG
4321 GTTAAAAAGG CTGAGGCAGG AGAATGGCGT GAACCCGGGA GGCGGAGCTT GCAGTGAGCT
4381 GAGATCATGC CACTGCACTC CAGCCTGGGC GACAGAGCGA GACTCTTGTC TCAAAATAAA
4441 AAACGTTTAC ATGTACATGT ATATTCAACA TGTACAAATA TAACCTATTC AAAAGTATTT
4501 ACTACATAAA TAGGTACTTA CATTACCTAT TTACTGTAAT AGTCAAAGCC TATGAAGTAT
4561 CTAACACTGA TGTGTAGGTA CTCACTTTGC TTGCCACTCT ATTAGGTGCT TTTTATGTTA
4621 TTTAATCATG AAGCCTGGCC ACAGGGTGCT TGTGCATTGA GTGTGGGAAC AAGATTACCA
4681 TCTCCCTTTT GAGGACACAG GCCTAGAGCA GTTAAGCAGC TTGCTGGAGG TTCACTGGCT
4741 AGAAAGTGGT CAGCCTGGGA TTTGGACACA GATTTTTCCA CTCCCAAGTC TGGCTGCTTT
4801 TTACTTCACT GTGAGGGGTA AAGGTAAATC AGCTGTTTTC TTTGTTCAGA AACTCTCTCC
4861 AACTTTGCAC TTTTCTTAAA GGAAAGTAAT GGACCAGTGA AGGTGTGGGG AAGCATTAAA
4921 GGACTGACTG AAGGCCTGCA TGGATTCCAT GTTCATGAGT TTGGAGATAA TACAGCAGGT
4981 GGGTGTTGTG CTGTGCTGGT GACCCATACT TGTTCACCCT AGTTAGATAA ACAGTAGAGT
5041 AGCCCCTAAA CGTTAAAACC CCTCAACTTG TTTTTGTTTT TGAGAAAGGG TCTTGCTCTG
5101 TCGCTCAGGC TGGAGTGCAG TGGCGCTGTG CGATCATGGC TGACCTTAGC CTTGACCTCC
5161 CAGGCTCCAT TGATCCTCAT GCCTTGGCCC GTAGCTGGGA CTACAGGTAC ACACCACCAC
5221 GCCTGGCTAA TTTTTGTATT TTTTTCTAGA GGTGGGGTTT CATCATGTTG CCCAGGCTGG
5281 TCTTGAACTG CTGGGCTCAA GTGGTCTATC CTCCTCGACC TCCCAAAGTG CTGGGATTAC
5341 ATGTGTGAGC CACTGTGCCT GGGAAAACCC TCAACTTTTC TTTTAAAAAA GAGGTCAACT
5401 TTATTGTATA TAAGCACTGT GCTAAAATTG CAGGAACTGG GACCATATCC TGATTTTTGT
5461 AATAATGCCA GCAGAGTACA CACAAGAAAA GTAACTGCAC TAGATTGTGA AGACTGGGGT
5521 GGACCTGCTT CTGAAGGTCC AGTGCCCTTT GTCTTAAGAT TTGGTGTAGT GTGTCTTTAG
5581 AAACCAAAAA AAGAGAAGAA GATCAACCTT AAGATTAGCC ACAAAACTGG GCTTTGATAC
5641 CTAGGTGTGG AAAAGAAAGG GAAAGAGTTG ATGTTTTGTC TTACAGCATC ATTGTAGAAG
5701 AGGGTGTTTT TTTGTTTGTT TGTTTTTTGA GACGGAGTCT TACTCTGTGG CCCAGGCTGG
5761 AGTGCAGTGG CGCGATCTCG GCTCACTGCA AGCTCCGCCT CCCGGGTTCA TGCCATTCTC
5821 CTGCCTCAGC CCCCTGAGTA GCTGGGACTA CAGGTGCCCG CCACCCCGCC TGGCTAATTT
5881 TTTGTATTTT TAGTAGAGAC GGGGTTTCAC TGTGTTAGCC AAGATGGTCT CTCTCCTGAC
5941 CTCGTGATCC GCCTGTCTCA GCCTCCCAAA GTGCTGGGAT TACAGGCATG AGCCACCGCA
6001 CCCAGCCAGA AGAGGGTGTT TTTTAAAGAA GGCAAATAGG AAATAAAAAC TTGGGCTCTT
6061 AACTTTTGTA ATGATCCCAG GTGTTTGAGC TGGGGGTTGA GGGTGGGTGC CTCGAGCAAA
6121 GGGGCTGCAT TTATTTGCAT AATGCCATGT AAGAGTAGCT CTACACCCCA AACACAGGCT
6181 TCTTAGTGGG ACCAAAGTAT GATACAAACT GAAGATGGAA TGCAGAGGAT TATTGGTACT
6241 TTGGAATATG CTTAAAAAAA ATTTTTTTAA AGTATTTTTA AAAAATCAGG CAACCCCTGA
6301 ACCAGAGTAG GTTCAGAGAA ACTGCCAAAT TTTATTTTCT TAATTTGGGA TTGGAAGCAA
6361 GTTAACAGAA GTTTATGAGT TAAGTTGCAT TTAGTGATCT TTTGCCATAT TTGAGTAATA
6421 ATCTGATTTT TTTGTTTATA GATTTCTTCT TAAATTAACT TTATTCATCT TGCTAATTTA
6481 GTTTCAAATA GTGATTTGTA ATGATCAGAT TTGATCCATT TCTGTAATTG CTGAAATTCC
6541 CCCGAGTTGC TTTTTGGCTT TACCGCCTCT GGTCTGGGAG GTGATTGCTC TGCTGCTTCC
6601 TGTAACTTGC CTGCCTTTCT CCCTGTGTGG GACTCCTGCG GGTGAGAGCG TGGCTGAAGA
6661 CAGCCGTGTT ATGAAAGGGC CTCCTGTGCT GTCGAGGTTG TGCTCTGTGA ATGTCATCCC
6721 CTGGTGCACA GCAGCACCTT CTACACAGGA TACAGTTGGA ATGCCGCCCC CTCGAGTTGT
6781 GTAAGGCAGC AGCCTTGGCC CTTGCACATA AGATGCTGTT GAATATTCTG CCTGCACCAA
6841 GTAAAGGGCA CAGATAGAAC TGCTTGGCAT ATGTTGCTGG GGAGATGAGT TTTTTGTAAA
6901 GTATACTACG TTCTTAAGAA TTTGGATCAT AACCATGGGA TTTTAATAAT AGAAAAACTG
6961 TTGAAGATCA GTCTGGTCCC TTATTTTTAC AGTGAAGAAG CCAAAGCCCA GAGAAGGGTG
7021 TTAACTTTAC AAGTGTCAGA CAGTAGTTAG AACTTGGTGG GGTTTTTTTT TTTTTTTTTT
7081 TGAGATGGAG TCTTGCTCTG TTGCCCAGGC TGGAGTGCAG TGGTGCGATC TCAGCTCACT
7141 GCAACCTCTG CCTCCCAGGT TCAAGCGATT CTCCTGCCTC AGCCTACTAA GTAGCTGGGA
7201 CTATAGGTGC GCACCACCAC GCCTAGCTAA TTTTTGTATT TTTTCAGTAG AGACAGGGTT
7261 TTGCTATGCT GGCCAGGCTG GTCTCAAACT CCTGACCTCA GATGATCCAG CCACCTCAGC
7321 TTCCCAAAGT GCTGGGGTTC CAGGTGTTAG CCACCATGCC TGGCCATAGA CTTGTTTCTG
7381 TTCCCTTCTC ACTGTGGCTG TACCAAGGTG TTGCTTATCC CAGAAGTCGT GATGCAGGTC
7441 AGCACTTTCT CCATGGGAAG TTTTAGCAGT GTTTCTTTTT AGAATGTATT TGGGAACTTT
7501 AATTCATAAT TTAGCTTTTT TTTCTTCTTC TTATAAATAG GCTGTACCAG TGCAGGTCCT
7561 CACTTTAATC CTCTATCCAG AAAACACGGT GGGCCAAAGG ATGAAGAGAG GTAACAAGAT
7621 GCTTAACTCT TGTAATAATG GCGATAGCTT TCTGGAGTTC ATATGGTATA CTACTTGTAA
7681 ATATGTGCTA AGATAATTCC GTGTTTCCCC CACCTTTGCT TTTGAACTTG CTGACTCATC
7741 TAAACCCCTG CTCCCAAATG CTGGAATGCT TTTACTTCCT GGGCTTAAAG GAATTGACAA
7801 ATGGGGACAC TTAAAACGAT TTGGTTTTGT AGCATTTATT GAATATAGAA CTAATACAAG
7861 TGCCAAAGGG GAACTAATAC AGGAAATGTC ATGAACAGTA CTGTCAACCA CTAGCAAAAT
7921 CAATCATCAT TGTGAAACAT AGGAAGCTTC TGTAGATAAA AAAAAAAATT GATACTGAAA
7981 ACTAGTCGAG ACTCCATTTA TATGTGTATG TTTTCTGAAA GCCTTTCAGA AAAATATTAA
8041 ATTTAAGGAC AAGATTTTTA TATCAGAGGC CTTGGGACAT AGCTTTGTTA GCTATGCCAG
8101 TAATTAACAG GCATAACTCA GTAACTGAGA GTTTACCCTT TGGTACTTCT GAAATCAGGT
8161 GCAGCCCCAT CTTTCTTCCC AGAGCATTAG TGTGTAGACG TGAAGCCTTG TTTGAAGAGC
8221 TGTATTTAGA ATGCCTAGCT ACTTGTTTGC AAATTTGTGT CTACTCAGTC AAGTTTTAAT
8281 TTAGCTCATG AACTACCTTG ATGTTTAGTG GCATCAGCCC TAATCCATCT GATGCTTTTT
8341 CATTATTAGG CATGTTGGAG ACTTGGGCAA TGTGACTGCT GACAAAGATG GTGTGGCCGA
8401 TGTGTCTATT GAAGATTCTG TGATCTCACT CTCAGGAGAC CATTGCATCA TTGGCCGCAC
8461 ACTGGTGGTA AGTTTTCATA AAAGGATATG CATAAAACTT CTTCTAACAT ACAGTCATGT
8521 ATCTTTTCAC TTTGATTGTT AGTCGCGGTT TCTAAAGATC CAGATAAACT GTACTTGCAG
8581 TTCAAATTAG GAAAAGCAAT TTTATTGGAC AATTACGGTG AAAATGAATT ATTTTATCTA
8641 GGTCAGTTAA GAACACTGTT CTGCTAAGAT GCAGTAAAAA GCAGGTTACA TTTGACCATA
8701 TTAGATCTGA GTTTGGAAAA CAGAAGTAGT CTTTAGTTTT AAAATGGCCA GATTTTCTTG
8761 CCAGGATTGG GTTTCTCACT TGTTAAACAG AACATTTTGT TAAGTTTAAA ACCTGGGATG
8821 GACTTAAGTA TTCATGTTCA TTCATGTTCA TTCAGGACTG CAGGTTATCA TGACTTGTTT
8881 AACTTGTGGG AAGCTGTTGT CCCAAGTTAT CCTGGGGAAC TGCATCTGGT TCTTGCAAAA
8941 CACCAAGTAG ACAGGCTCTC TTTTACCTCC CCTTGAGGGC ATTAACATTC AGTAGTCACT
9001 TCCATTCAGT TAACCCTTTA TTTTTATGGT TTTTCTTGAG CCATAGTTGT AAAGCAGAAA
9061 AATCATTTAT AAAGGTTTGT TGAACAAAAT TCAAAATACT GTTGCTTAAA GTATTAAGAT
9121 TTTTTAGGAT TATACCTTAC TTATAGGCCC GTCATTCATT TGGCATGAAA TTTTGAGTTT
9181 TATTCACTTT CACTTTCCTT TTTTTCCAAA GCAATTAAAA AAACTGCCAA AGTAAGAGTG
9241 ACTGCGGAAC TAAGGTTACT GTAACTTACC ATGGAGGATT AAGGGTAGCG TGTGGTGGTC
9301 TACAACATAG TTATTTGGGT TTTAGTATTT CATTTAGACA GCAACACTTA CCTAATGTTT
9361 AAAGGTAATG TCTTTGCAAC ACCAAGAAAA AGCTTTGAGT AGTAGTTTCT ACTTTTAAAC
9421 TACTAAATAT TAGTATATCT CTCTACTAGG ATTAATGTTA TTTTTCTAAT ATTATGAGGT
9481 TCTTAAACAT CTTTTGGGTA TTGTTGGGAG GAGGTAGTGA TTACTTGACA GCCCAAAGTT
9541 ATCTTCTTAA AATTTTTTAC AGGTCCATGA AAAAGCAGAT GACGGGCAAA GGTGGAAAGA
9601 CTACAAAGAC GATGACGACA AGTGATGGAA GTCGTTTGGC TTGTGGTGTA ATTGGGATCG
9661 CCCAATAAAC ATTCCCTTGG ATGTAGTCTG AGGCCCCTTA ACTCATCTGT TATCCTGCTA
9721 GCTGTAGAAA TGTATCCTGA TAAACATTAA ACACTGTAAT CTTAAAAGTG TAATTGTGTG
9781 ACTTTTTCAG AGTTGCTTTA AAGTACCTGT AGTGAGAAAC TGATTTATGA TCACTTGGAA
9841 GATTTGTATA GTTTTATAAA ACTCAGTTAA AATGTCTGTT TCAATGACCT GTATTTTGCC
9901 AGACTTAAAT CACAGATGGG TATTAAACTT GTCAGAATTT CTTTGTCATT CAAGCCTGTG
9961 AATAAAAACC CTGTATGGCA CTTATTATGA GGCTATTAAA AGAATCCAAA TTCAAACTAA
10021 ATTAGCTCTG ATACTTATTT ATATAAACAG CTTCAGTGGA ACAGATTTAG TAATACTAAC
10081 AGTGATAGCA TTTTATTTTG AAAGTGTTTT GAGACCATCA AAATGCATAC TTTAAAACAG
10141 CAGGTCTTTT AGCTAAAACT AACACAACTC TGCTTAGACA AATAGGCTGT CCTTTGAAAG
10201 CTTTAGGGAA ATGTTCCTGC TTAGTCATTT TAGCATTTTG ATTCATAAAG TACCTCCTCA
10261 TTTTAAAAAG ACATTATGAT GTAAGAGAGC CATTTGATAA CTTTTTAGTG AGCTTTGAAA
10321 GGCAAGTTAC AGCCTCAGCT AGCTAGTAAG ATTATCTACC TGCCAGAATG GCACAAATTC
10381 TACATTCAAG GGTAGACGCT GGCACAACCT ACTTACAGAT TAGCCCTTTA AAGCAATCTG
10441 TAGCATTAGA AGATGGAACC AAGGAAATGT TTGACTGTGG GTTCTGGCTG TTGAGAAATA
10501 ATTTACACAC CGAATTAGTG AAATGAGTCA CTTTCTCTTA ATGTATTTAT GTACCTGAGA
10561 GAATGCTTTT CAATGTTAAC CTAACTCAGG TTTGACTAAA TTATTCAATT GGAAATTGTA
10621 GAATATTATT TCTGATAAAC CAGAAATAAG TGAAATGCTG TTTGTTCATA AATATGTACT
10681 TTATCAAATG TAGGAGAGAT CATTTAGGAG AGGAAAAGCT AAATTGGAAG ACAAATCTGT
10741 AGTGTTTCCA AAGTTTTAAA ATTATGGTAA ACAACAGTAT GTTCACAGTA AGTGGTTAAA
10801 ACAACCATTC TTTAAATCTC AGTAGAGAAT TTTTAAAAAG CAGTATTTAA CACATTTCCC
10861 TAATGTAGTT TGTTGCCTAT GTGGAATAAC TCAATTAGAG ACTCACTTAT GCCTTTTGAA
10921 ACTTCAAATA TAATTACACT ACCAGTTTTT ACATGTGCAT ATAGGATGGT CCCAATACTT
10981 TAAATTGGAA ATACAGGCTG TAAGTCCTTC AAGTCTGGAT GTTGGGTAAT CACGTTTTCT
11041 TCCAGAAGCC ATTTGTTAGG ACTTTAAAAC TTCTCAGTGG GCCAGTGTAA AATTAAGGAC
11101 AAGTTTTATA ATTTAAATTT ACAGATAAAT ATAAAACAAT TTTCTCTCAT TTTCTAAAGG
11161 CAGGAATATA AGGACATTGC CCTAGATATA TTCTCCCCAT CAAACCAAAG GTCTTGCTGC
11221 TGAGTATTAT TTAAAAACCA GAGTAATAAA GCAGCTTCGC CTGAGGATGA AGGCAAAGAC
11281 AAAGTTAACT GCTCAGCATA CTGAAACCAG GAGTTTGAAA AAGTAATTCA AAACAACTTC
11341 AAGGCCATAA ATACTTGGAT AGTGTGACAT CAGGTCTTGG CACTGGATTT CCTGCAATTT
11401 CAGATGAAGG CTTGACTTGG CTTTAGATGT TTTCATGTGA TAATTCTTGG ATAACCTACA
11461 ACGATTTTTC CTATTTAACC CACCAACTTT AAGCAAAAGA AAGAACGTAT ATGTGGGGGA
11521 AAAGTTCGTT TACCTCTGCC CCGCAGGGGA ATTAAAACGC TGGTGAAGAA AGGCAACAGG
11581 CAAGGCTTAT ATAAGGAAAC AGTAGTAGGC TGCACTGAAG TCCTCAAACA CAACAAAAAG
11641 CTCTTAGGTT GCAAATGTTA ACCTTGATTC TTTTACCCTT TTGAAAAATT CAATGGGATG
11701 GTTGGAAAAA AAAAAAACCA CAGACACACA CACACACACA CGCAACCTTC TAACGTAATA
11761 CCCAGGCAGT CCGATAATTT ATAGTACAAC GTGTAACACT GGGATCCTCT AGAGTCGACC
11821 TGCAGGCATG CAAGCTTGGC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT
11881 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC
11941 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGC
//
Claims (2)
- 配列番号3又は配列番号5で表される、エキソン5において2塩基の欠失を有するSOD1ゲノムDNAを導入した、家族性筋萎縮性側索硬化症(FALS)発症するトランスジェニックマウス。
- 前記2塩基が、SOD1遺伝子のエキソン5のコドン126の、2個のチミンである、請求項1に記載の家族性筋萎縮性側索硬化症(FALS)発症するトランスジェニックマウス。
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JPH09509046A (ja) * | 1993-11-29 | 1997-09-16 | ユニヴァーシティ オブ サウス フロリダ | トランスジェニックマウス |
JP2003524393A (ja) * | 1999-02-10 | 2003-08-19 | エラン ファーマシューティカルズ,インコーポレイテッド | βセクレターゼ酵素組成物および方法 |
JP2003535605A (ja) * | 2000-06-20 | 2003-12-02 | ザ ガバニング カウンシル オブ ザ ユニバーシティ オブ トロント | 神経変性性障害のトランスジェニック動物モデル |
JP2002142610A (ja) * | 2000-11-07 | 2002-05-21 | Tohoku Techno Arch Co Ltd | Alsモデルラット |
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