JP4537573B2 - 熱安定ルシフェラーゼと生産方法 - Google Patents

熱安定ルシフェラーゼと生産方法 Download PDF

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JP4537573B2
JP4537573B2 JP2000511874A JP2000511874A JP4537573B2 JP 4537573 B2 JP4537573 B2 JP 4537573B2 JP 2000511874 A JP2000511874 A JP 2000511874A JP 2000511874 A JP2000511874 A JP 2000511874A JP 4537573 B2 JP4537573 B2 JP 4537573B2
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luciferase
synthetic
plate
mutagenesis
beetle
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JP2001516585A (ja
JP2001516585A5 (https=
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ウツド，キース・ブイ
ホール，メアリー・ピー
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プロメガ・コーポレイシヨン
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2000511874A 1997-09-19 1998-09-18 熱安定ルシフェラーゼと生産方法 Expired - Lifetime JP4537573B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5937997P 1997-09-19 1997-09-19
US60/059,379 1997-09-19
PCT/US1998/019494 WO1999014336A2 (en) 1997-09-19 1998-09-18 Thermostable luciferases and methods of production

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2010006999A Division JP5580607B2 (ja) 1997-09-19 2010-01-15 熱安定ルシフェラーゼと生産方法

Publications (3)

Publication Number Publication Date
JP2001516585A JP2001516585A (ja) 2001-10-02
JP2001516585A5 JP2001516585A5 (https=) 2006-01-05
JP4537573B2 true JP4537573B2 (ja) 2010-09-01

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JP2000511874A Expired - Lifetime JP4537573B2 (ja) 1997-09-19 1998-09-18 熱安定ルシフェラーゼと生産方法
JP2010006999A Expired - Lifetime JP5580607B2 (ja) 1997-09-19 2010-01-15 熱安定ルシフェラーゼと生産方法

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JP2010006999A Expired - Lifetime JP5580607B2 (ja) 1997-09-19 2010-01-15 熱安定ルシフェラーゼと生産方法

Country Status (6)

Country Link
US (1) US20030068801A1 (https=)
EP (2) EP2860252A1 (https=)
JP (2) JP4537573B2 (https=)
AU (1) AU754312C (https=)
CA (1) CA2301828A1 (https=)
WO (1) WO1999014336A2 (https=)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2301828A1 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
US6602677B1 (en) * 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) * 1998-10-28 1998-12-23 Secr Defence Novel enzyme
AU6094100A (en) * 1999-07-13 2001-01-30 Heska Corporation Fc epsilon receptor-luminescence inducing protein chimeric nucleic acid molecules, fusion proteins and uses thereof
GB9925161D0 (en) * 1999-10-26 1999-12-22 Secr Defence Novel enzyme
NZ518413A (en) * 1999-10-26 2004-11-26 Secr Defence Novel enzyme
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7083911B2 (en) 2001-02-16 2006-08-01 Promega Corporation Method for detection of ATP
GB0111275D0 (en) 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
AU2003268489B2 (en) 2002-09-06 2008-01-24 Promega Corporation Method for detecting transferase enzymatic activity
US7413874B2 (en) 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
JP4485470B2 (ja) 2002-12-23 2010-06-23 プロメガ コーポレイション ルシフェラーゼ酵素活性を保護するための方法及びキット
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
CA2541765A1 (en) 2003-10-10 2004-10-01 Promega Corporation Luciferase biosensor
US7422868B2 (en) * 2004-07-02 2008-09-09 Promega Corporation Microbial ATP extraction and detection system
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
GB0505763D0 (en) * 2005-03-22 2005-04-27 Enigma Diagnostics Ltd Polymorphism detection method
GB0517005D0 (en) 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2327768B1 (en) * 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
WO2009142735A2 (en) 2008-05-19 2009-11-26 Promega Corporation LUCIFERASE BIOSENSORS FOR cAMP
EP2326953B1 (en) 2008-07-22 2018-03-21 Promega Corporation Adp detection based luminescent phosphotransferase or atp hydrolase assay
LT3409764T (lt) * 2009-05-01 2020-06-10 Promega Corporation Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US8735559B2 (en) 2010-05-11 2014-05-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
BR112013010487B1 (pt) 2010-11-02 2021-02-02 Promega Corporation compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro
WO2012061477A1 (en) 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
IN2014KN00742A (https=) * 2011-09-09 2015-10-02 Quidel Corp
US9487814B2 (en) 2013-02-22 2016-11-08 Promega Corporation Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
EP3204509B1 (en) 2014-10-08 2019-07-10 Promega Corporation Bioluminescent succinate detection assay
JP6688499B2 (ja) * 2014-10-27 2020-04-28 国立大学法人 鹿児島大学 安定性が増加したウミシイタケ（Ｒｅｎｉｌｌａｒｅｎｉｆｏｒｍｉｓ）由来ルシフェラーゼ変異体
CN105200022B (zh) * 2015-11-03 2018-11-16 南京工业大学 萤火虫荧光素酶热稳定性改造的方法
EP3219722B1 (en) * 2016-03-14 2019-03-27 Hung Guang Biotech Co. Ltd. Modified colostrum protein and application thereof
WO2020009215A1 (ja) * 2018-07-06 2020-01-09 キッコーマン株式会社 ルシフェラーゼ変異体
CN110456052A (zh) * 2019-08-22 2019-11-15 中国科学院武汉病毒研究所 一种血清中梅毒螺旋体抗体的快速检测方法及其应用
JPWO2024158050A1 (https=) 2023-01-27 2024-08-02

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3048466B2 (ja) * 1991-06-27 2000-06-05 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
US5229285A (en) * 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
GB9501170D0 (en) * 1994-03-23 1995-03-08 Secr Defence Luciferases
DE69633171T2 (de) * 1995-01-20 2005-08-18 The Secretary Of State For Defence, Salisbury Mutierte luziferasen
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
CA2301828A1 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production

Also Published As

Publication number Publication date
WO1999014336A3 (en) 1999-06-03
AU754312C (en) 2005-11-10
EP1015601B1 (en) 2015-01-07
AU9492198A (en) 1999-04-05
EP2860252A1 (en) 2015-04-15
JP2001516585A (ja) 2001-10-02
WO1999014336A2 (en) 1999-03-25
US20030068801A1 (en) 2003-04-10
AU754312B2 (en) 2002-11-14
JP2010104377A (ja) 2010-05-13
JP5580607B2 (ja) 2014-08-27
EP1015601A2 (en) 2000-07-05
CA2301828A1 (en) 1999-03-25

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