JP4494167B2 - Sample inspection plate - Google Patents

Description

The present invention is a lightweight, breakage hardly, not bulky, relates inexpensive sample testing plate can be easily be multiple sample testing, and more particularly, especially, good optimal sample pathogenic E. coli immune serum agglutination It relates to an inspection plate.

Recently, the lifestyle has changed due to urban intensive life, expansion of distribution system, imported foods and imported foods, etc., causing diversification, enlargement and widening of food poisoning.
As a modern approach to food poisoning and infectious enteritis, prompt response to test results is indispensable. Pathogenic Escherichia coli is a gram-negative bacilli belonging to the family Enterobacteriaceae included in the normal intestinal flora of humans, and is a causative bacterium of intestinal infections such as diarrhea, acute gastroenteritis or colitis. Pathogenic Escherichia coli is roughly classified into four types: enteropathogenic E. coli, intestinal invasive Escherichia coli, enterotoxic Escherichia coli, and enterohemorrhagic Escherichia coli, depending on the pathogenic mechanism. Each pathogenic E. coli is examined for a specific serotype to estimate the pathogenic E. coli.

As this type, conventionally, a drained slide glass (76 x 26 ml) is used and divided into 8 sections with a glass pencil, or the boundary line is partitioned with white paint as a water drained slide glass with 8 frame numbers. A split one is used (for example, see Non-Patent Document 1). As a feature of this, microscopic observation is possible, and E. coli suspension is dropped into each compartment, mixed serum is added dropwise, and the presence or absence of an agglutination reaction is observed and determined.
"Slide glass for bacteria test" (Matsunami Glass Industry Co., Ltd. catalog)

  However, the above drained slide glass may accidentally cut fingers during operation, and further, it is heavy and easily damaged, and is bulky. There are many problems such as easy to cause and uncertain determination, and many problems are included in terms of workability, efficiency, worker safety, and the like.

  In addition, it is expensive because it is made of glass, and therefore it is often not reused but cleaned and reused. There are also problems such as adversely affecting test results.

  In view of such circumstances, the present invention eliminates the above-mentioned problems of the prior art, prevents contamination of the specimen, is lightweight, is not easily damaged, has high worker safety, and is suitable for disposable use. An inspection plate is provided.

Claims of the present invention to solve the above problems 1, sampling portion which is surrounded by at least one raised wall on the synthetic resin sheet is provided at least one, the bottom of the sample collection unit 1 It comprises a sample test plate for pathogenic E. coli immune serum agglutination reaction characterized by a flat surface inclined at ˜5 degrees .

  According to a second aspect of the present invention, the sample inspection plate according to the first aspect includes a plurality of sample collection portions.

  A third aspect of the present invention includes the sample inspection plate according to the first or second aspect, wherein the sample collection portion is rectangular or elliptical.

A fourth aspect of the present invention includes the sample inspection plate according to any one of the first to third aspects, wherein an identification symbol is provided in the vicinity of the sample collection portion.

A fifth aspect of the present invention includes the sample inspection plate according to any one of the first to fourth aspects, wherein a recording unit is provided.

A sixth aspect of the present invention includes the sample inspection plate according to any one of the first to fifth aspects, wherein the sample collection portion is surrounded by two inner and outer raised walls.

A seventh aspect of the present invention includes the sample inspection plate according to any one of the first to sixth aspects, wherein a raised frame is provided around the outer periphery.

The eighth aspect of the present invention includes the sample inspection plate according to any one of the first to seventh aspects, in which a grip portion is extended.

Claim 9 of the present invention, any of the sampling unit 8 becomes, according to claim 8 in which the identification means between the 1st sampling portion of the first second sampling portion is subjected The sample inspection plate described in item 1 is included.

Claim 10 of the present invention, claim identification means and the first sampling portion of the first second sampling portion is made of a wider spacing than the spacing of the second to eighth-th sampling portion 9 It contains the sample inspection plate described.

The eleventh aspect of the present invention includes the sample inspection plate according to any one of the first to tenth aspects formed by vacuum forming.

A twelfth aspect of the present invention includes the sample inspection plate according to any one of the first to tenth aspects formed by injection molding.

  The sample inspection plate of the present invention is provided with at least one sample collection part surrounded by a raised wall on a synthetic resin sheet, so that it is lighter than conventional glass ones, is not easily damaged, is not bulky, Very safe for the operator.

Moreover, the sample collection part consists of a flat surface whose bottom is inclined by 1 to 5 degrees. Thereby, in the pathogenic E. coli immune serum agglutination reaction, the mixed solution of E. coli suspension and mixed serum or plain serum is moved up and down or left and right within the sample collection part to determine the presence or absence of aggregation. It becomes easy to move.

  In addition, since the sample collection part is surrounded by the raised wall, the sample does not leak out. Especially when a plurality of sample collection parts are provided, the adjacent sample collection parts are clearly divided by the raised wall. Therefore, it is possible to prevent the samples of the adjacent sampling units from being mixed with each other, and it is possible to prevent a trouble that the determination is uncertain due to contamination.

Further, the sample collection part is preferably rectangular or elliptical. As described above, in the pathogenic E. coli immune serum agglutination reaction, a mixed solution of E. coli suspension and mixed serum or plain serum is moved up and down or left and right in the sample collection unit to determine the presence or absence of aggregation. because, in order to ease Ku take the distance to move.

  In addition, when two raised walls forming the sample collecting part are provided, for example, double inside and outside, the sample leaking from the collecting part is first a recess between the adjacent inner raised wall and the outer raised wall. Even if it leaks from the concave portion, it is preferable because it is blocked by the outer raised frame and the leakage is prevented.

  In addition, since the desired number of sample collection units can be easily provided on the synthetic resin sheet, a plurality of samples can be collected on a single plate at the same time, and the workability and efficiency of inspection are greatly improved. It is done.

  Further, by providing an identification symbol in the vicinity of the sample collection unit, each sample collection unit can be clearly distinguished, and the workability and reliability of the inspection can be improved.

  Further, by providing the recording unit, the name, number, symbol, etc. of the sample provider can be described, and the workability and reliability of the inspection can be improved.

  Further, by providing a raised frame around the outer periphery of the sample collection plate, the sample leaking from the collection part is blocked by the raised frame, and leakage from the plate is prevented. Further, by providing a raised frame, rigidity is imparted even when a thin synthetic resin sheet is used, so that the amount of raw material resin can be saved and an inexpensive sample inspection plate can be provided.

  Further, by providing the grip portion, the plate can be easily handled and the workability is greatly improved. In this case, preferably by providing a raised grip part, even if the sample leaks from the collection part, it is hygienic without touching the hand.

Moreover, because of the aggregation determination of mixed-serum provided eight sampling unit, for aggregate determination using the following plain serum, mix serum for control the first-th sampling portion It is preferable that the second and subsequent sampling parts are used for simple serum, and that the first mixed serum and the second and subsequent simple serum are not confused with each other. Preferably, as such a distinction, if the interval between the first sample collection unit and the second sample collection unit is wider than the interval between the second to eighth sample collection unit, It is preferable in that it can be performed simultaneously with molding and can be distinguished at a glance.

  The sample inspection plate of the present invention can be provided easily and inexpensively by producing a synthetic resin by vacuum molding or injection molding.

Sample testing plate of the present invention are for pathogenic E. coli immune serum agglutination, sampling portion which is surrounded by at least one raised wall on the synthetic resin sheet is provided at least one, of the sample collection unit The bottom is a flat surface inclined at 1 to 5 degrees .

  The synthetic resin used in the present invention is not particularly limited as long as it is excellent in optical properties such as transparency and chemical resistance, and has low water absorption. For example, polyethylene terephthalate, polybutylene terephthalate, polynaphthalene terephthalate, etc. Examples include polyester, polycarbonate, polyamide, polyarylate, and the like. Among these, polyester is preferable in terms of transparency and economy.

  On the synthetic resin sheet, a sample collection unit surrounded by a raised wall is provided. The shape of the sampling part may be any of a rectangle, a circle, an ellipse, a polygon, etc., and its size is not particularly limited, but usually one side is rectangular if it is a rectangle, and the diameter is oval if it is a circle. In that case, the major axis or the minor axis is preferably about 8 to 20 mm. If it is too small, it is difficult to collect a sample. On the other hand, if it is too large, it is meaningless. On the other hand, handling becomes worse and material costs are increased, which is uneconomical.

In particular, pathogenic E. coli immune serum agglutination reaction, since determining the presence or absence of agglutination with the naked eye by moving the mixed solution of E. coli bacteria cloudy solution mixed serum or plain serum vertically or horizontally, taking the distance to move A rectangular or elliptical sample collection part is preferred. In this case, a rectangular shape or an elliptical shape having a long side or a long diameter of 8 to 25 mm and a short side or a short diameter of 3 to 8 mm is preferable from the viewpoint of the amount of liquid and workability to be determined.

  The raised wall is used to block the sample so that it does not leak out from the sampling part, and is provided with at least one (single layer). The upper limit is not particularly limited, but is preferably about three (triple) from the viewpoint of practicality, and more preferably two on the inner side and the outer side (double).

  The raised wall preferably has a width of about 0.5 to 1.5 mm and a height of about 0.5 to 5.0 mm. When providing a plurality of raised walls, the interval is preferably about 1.5 to 3 mm.

The sample inspection plate of the present invention is preferably a rectangle having a side of about 50 to 200 mm, preferably about 50 to 150 mm, and a thickness of about 1.0 to 5 mm in terms of workability and handling.
The sample inspection plate is provided with at least one sample collection unit, but it is preferable to provide a plurality of sample collection units from the viewpoint of workability and efficiency, and more preferably three or more. The upper limit is not particularly limited, but if it is too large, the size of the inspection plate is inevitably increased, and workability and handleability deteriorate, so about 20 is preferable. For example, in the case of pathogenic E. coli immune serum agglutination reaction, it is preferable to provide eight.

Further, pathogenic E. coli immune serum agglutination reaction is to observe the first presence of aggregates in a mixed serum (mixed 1-8), then, if it is determined that the positive mixed sera, a plain serum constituting the mixture Serum Similarly, the presence or absence of aggregation is determined.
Therefore, 8 sample collection parts are required for the determination of aggregation of the former mixed serum, and for the aggregation determination using the latter simple serum, for example, the first sample collection part is used as a control. Therefore, it is preferable to use the second and subsequent sampling parts for plain serum. Further, it is preferable to distinguish the first mixed serum and the second and subsequent simple sera so as not to be confused. For such distinction, the first sampling section and the second sampling section are distinguished. The interval between the first sample collection unit and the second sample collection is set to be slightly wider than the interval between the other sample collection units, and a division line is drawn. , Etc. are preferred.

Further, pathogenic E. coli immune serum agglutination reaction is a mixture of a mixed serum or plain serum and E. coli bacteria cloudy solution is moved in a sampling portion (flowing a bottom surface) so determined with the naked eye, to facilitate moving For this reason, the bottom portion is a flat surface inclined. In the case of a rectangle, the inclination is in the direction of the long side, and in the case of an ellipse, the inclination is in the direction of the long diameter, and the inclination angle is 1 to 5 degrees, preferably 1 to 3 degrees.

In order to distinguish each sample collection unit in the vicinity of the sample collection unit, an identification symbol such as a number or alphabet is provided, or a recording unit is provided for recording the name, number, symbol, etc. of the sample provider. Therefore, workability and reliability can be improved. The identification symbol can be provided by printing or the like, but it is preferable to engrave it in a convex shape or a concave shape simultaneously with molding. The recording unit is provided, for example, by roughening the surface or making a satin finish in order to improve the writing property.
In addition, markings such as changing the roundness of the corners of the synthetic resin sheet and making marks so that the top and bottom of the sample inspection plate can be distinguished at a glance are provided to improve workability and reliability of inspection. Is also possible.

  By providing a raised frame around the outer periphery of the sample collection plate of the present invention, the sample leaking from the collection part is blocked by the raised frame, and leakage outside the plate is prevented. Further, since rigidity is imparted even if a thin synthetic resin sheet is used, the amount of resin can be saved, and an inexpensive sample inspection plate can be provided.

In addition, the sample inspection plate of the present invention is provided with a gripping portion, thereby improving handling and improving inspection workability. The gripping part may be provided in the outer peripheral raised frame, or may be extended outside the raised frame. In the case of being provided in the outer peripheral raised frame, it is preferable that a protruding wall or a concave groove is interposed between the sample collection part or the sample collection part so as to prevent the leaked sample from touching the hand.
The shape in the case of extending the grip part outside the raised frame is not particularly limited. It is preferable that the gripping portion is formed by extending it obliquely upward at a predetermined angle during use.

  The sample inspection plate of the present invention is easily molded by injection molding, compression molding, vacuum molding, pressure forming, etc. In particular, since the vacuum molding can be molded using a thin synthetic resin sheet (film), the amount of raw resin This is preferable in that a sample inspection plate that is light and inexpensive can be provided.

  The sample inspection plate of the present invention as described above can be provided with a cover as required. The cover has effects such as prevention of sample evaporation, dust contamination, and leakage. Accordingly, the cover needs to be of a size sufficient to cover at least the sample collection part. The cover is preferably a synthetic resin film having adhesiveness to be in close contact with the sample inspection plate and having a moisture barrier property and transparency that does not allow moisture to permeate. Examples of such a film include polyethylene, biaxially stretched polypropylene, moisture-proof cellophane, Examples include polyester, polyamide, ethylene-vinyl alcohol copolymer resin, vinyl chloride resin, and vinylidene chloride resin. The thickness of the cover film is not particularly limited, but is preferably about 50 to 300 μm.

If necessary, the cover may be detachably bonded to the plate with adhesive and pressure-sensitive adhesives and pressure-sensitive adhesives. In this case, the adhesive or pressure-sensitive adhesive and release paper are laminated in this order. When bonding or temporary (weak) bonding is desired, it is convenient to peel off the release paper and expose the adhesive or pressure-sensitive adhesive to allow temporary (weak) bonding. Further, one end may be bonded to one end of the sample inspection plate by heat sealing, an adhesive, or an adhesive, and the other end of the cover may be freely opened and closed.
In addition, the opening and closing operation of the cover is facilitated by partially cutting off the end portion of the synthetic resin sheet so that the portion is only the cover, or by partially protruding so that the cover can be easily picked up with fingers. be able to.

  Hereinafter, the present invention will be described in more detail with reference to the drawings illustrating examples. However, the present invention is not limited to the examples.

Example 1
FIG. 1 shows a sample inspection plate according to the present embodiment. FIG. 1A is a top view, FIG. 1B is an AA sectional view , and FIG . 1C is an BB enlarged sectional view. It is.
In these drawings, the sample inspection plate 1 is formed by vacuum forming, and eight sample collection parts 4 surrounded by the raised walls 3 are formed on the surface of the synthetic resin sheet 2, and the raised frame 5 is provided around the outer periphery. Further, a raised gripping part 6 and / or recording part 9 is provided on one side on the short side.

  In the present embodiment, the raised wall 3 is formed in a tapered shape with the sample collection part 4 side widened so that the sample does not stay in the corner of the sample collection part 4. In addition, a number is provided as an identification symbol (recessed in this example) in the upper part of each sample collection unit 4, whereby each sample in each sample collection unit is identified.

  In the sample inspection plate 1, a sample such as E. coli suspension separated from blood and stool is collected in the sample collection unit 4. The sample leaking out from the collecting part 4 is blocked by the outer raised frame 5. In this case, since the grip part 6 and / or the recording part 9 are formed so as to be raised, the finger is not contaminated by the leaked sample. In the recording unit 9, a sample provider or the like can be recorded.

The bottom 4a of the sample collection unit 4 is an inclined flat surface. That is, as shown in FIG. 1C, the bottom 4a of the sample collection unit 4 is inclined by about 2 degrees in the long side direction (in FIG. 1, the upper identification symbol 11 side is high and the lower part is low. Is indicated by two lines).
By configuring in this way, for example, when the pathogenic E. coli immune serum agglutination reaction is observed using the sample inspection plate 1 of the present embodiment, a mixture of the E. coli suspension and mixed serum or plain serum is collected. By moving (flowing) on the bottom 4a of the portion 4, it becomes easy to observe the aggregation reaction with the naked eye.

Example 2
FIG. 2 is a top view showing the sample inspection plate of the present embodiment, which is the same as that of the first embodiment except that the grip portion 6 is provided on the other side of the short side, that is, on the right side.
The sample inspection plate 1 of the present embodiment is suitable for holding the grip portion 6 with the right hand while the sample inspection plate 1 of the embodiment 1 grips the grip portion 6 with the left hand.

Example 3
FIG. 3 shows the sample inspection plate of the present example. In the sample inspection plate 1 of Example 1, the sample collection unit 4A with the identification symbol “1” and the identification symbol “2” are attached. An example in which the interval between the sample collection unit 4B is increased so that the sample collection unit 4A with the identification symbol “1” can be distinguished from the sample collection unit 4B with the identification symbol 2-8. Show.
With this configuration, for example, when agglutination determination using simple serum is performed, the sample collection unit 4A is used for mixed serum for control, and the remaining seven sample collection units 4B are used as simple serum. By using it for the purpose, the trouble that both are confused is prevented.

Example 4
FIG. 4 shows a sample inspection plate according to the present embodiment. The sample inspection plate 1 according to the first embodiment is the same as the first embodiment except that a recording section 12 is provided above each sample collection section 4. It is.

Example 5
Figure 5 shows a sample testing plate of the present embodiment, FIG. 5 (a) is a top view, FIG. 5 (b) C - at C cross-sectional view, in the sample testing plate 1 of Example 1, the cover 7 The rest is the same as in the first embodiment.
That is, in this example, the hinge 8 is formed by connecting the side of the short side of the cover 7 made of a synthetic resin film that is not provided with the grip 6 and / or the recording unit 9 to the raised frame 5 by heat sealing. In the present embodiment, the grip 7 and / or the recording unit 9 side of the cover 7 is slightly lengthened to provide the protruding edge 7a. Therefore, the cover 7 can be easily opened and closed by grasping the protruding edge 7a. .
In the sample inspection plate 1 of the present embodiment, the cover 7 is opened via the hinge 8 and the sample is collected at the sample collection unit 4, and then the cover 7 is attached to prevent the sample from evaporating or dust. Can be prevented.

Example 6
Figure 6 shows a sample testing plate of the present embodiment, in the sample testing plate of Example 1, except that a grip portion 13 provided to extend is the same as in Example 1.
That is, a tongue-like (semicircular) gripping portion 13 is extended on the outer peripheral raised frame 5 on the opposite side of the gripping portion 6 and / or the recording portion 9 of the sample inspection plate 1. A fold line 14 is engraved in the vicinity of the raised frame 5 of the gripping part 13, and if necessary, the fold line 14 is erected at a desired angle obliquely upward or substantially upright through the fold line 14 so that it can be easily picked.

Example 7
Figure 7 shows a sample testing plate of the present embodiment, in the sample testing plate of Example 3, except that a grip portion 13 provided to extend is the same as in Example 3.
That is, a rectangular gripping piece is extended on the outer peripheral raised frame 5 on the opposite side of the gripping portion 6 and / or the recording portion 9 of the sample inspection plate 1, and the substantially triangular gripping portions 13 a and 13 b are connected by the folding line 14. The gripping portions 13a and 13b are formed to stand up obliquely upward or substantially upright at a desired angle via the fold line 14 so that they can be easily picked.

Example 8
Figure 8 shows a sample testing plate of the present embodiment, FIG. 8 (a) top view, FIG. 8 (b) D - a D cross-sectional view.
In these drawings, the sample inspection plate 1 is formed by injection molding, and the sample collection parts 4 that are doubly surrounded by the inner raised wall 3a and the outer raised wall 3b on the surface of the synthetic resin sheet 2 are arranged in 8 rows. Each is formed, and a raised frame 5 is provided on the outer periphery, and gripping portions 6 are provided on both sides on the short side.

  Also, a number is engraved as an identification number in the upper part of each sample collection unit 4, thereby identifying the sample in each sample collection unit.

  In the sample inspection plate 1, a sample such as E. coli suspension separated from blood and stool is collected in the sample collection unit 4. The sample leaking from the sampling portion 4 first remains in the recess 10 between the inner raised portion 3a and the outer raised wall 3b, and leakage from the sampling portion 4 is prevented. It is blocked by the peripheral raised frame 5.

Example 9
FIG. 9 shows a sample inspection plate according to the present embodiment. In the sample inspection plate according to the first embodiment, an example in which the sample collection unit 4 has an elliptical shape is shown. The effect is substantially the same as in the first embodiment.

Example 10
FIG. 10 shows a sample inspection plate according to the present embodiment. In the sample inspection plate according to the third embodiment, an example in which the sample collection unit 4 has an elliptical shape is shown. The effect is substantially the same as in the third embodiment.

  As described above, since the sample inspection plate of the present invention is made of a synthetic resin sheet, it is lightweight, hard to break, thin and not bulky, and is excellent in safety and handling.

Moreover, since the sample collection part consists of a flat surface whose bottom is inclined by 1 to 5 degrees, in the pathogenic E. coli immune serum agglutination reaction, the mixed liquid can be easily moved, and the E. coli suspension and the mixed serum or simple serum It becomes easy to determine the presence or absence of agglomeration by moving the mixed solution up and down or left and right in the sample collection section.

  In addition, since the sample collection part is surrounded by the raised wall, the sample does not leak out. Especially when a plurality of sample collection parts are provided, the adjacent sample collection parts are clearly divided by the raised wall. Therefore, it is possible to prevent the samples of the adjacent sampling units from being mixed with each other, and it is possible to prevent a trouble that the determination is uncertain due to contamination.

  In addition, since the sample inspection plate of the present invention can process a large number of samples at a time by providing a plurality of sample collection units, workability and efficiency are dramatically improved. The sample inspection plate of the present invention is particularly suitable as a general-purpose plate for pathogenic E. coli immune serum agglutination reaction.

  In addition, since the sample inspection plate of the present invention can be easily formed by vacuum molding, injection molding, or the like, an inexpensive sample inspection plate can be provided, and is particularly suitable for disposable use.

The plate for a sample test | inspection of Example 1 is shown, (a) is a top view, (b) is AA sectional drawing , (c) is BB sectional drawing . 6 is a top view showing a sample inspection plate of Example 2. FIG. It is shown to the top view of the specimen inspection plate of Example 3. 10 is a top view showing a sample inspection plate of Example 4. FIG. It shows the sample testing plate of Example 5, is (a) is a top view, (b) is sectional view taken along line C-C. It is shown to the top view of the specimen inspection plate of Example 6. 10 is a top view showing a sample inspection plate of Example 7. FIG. It shows the sample testing plate of Example 8, (a) is a top view, (b) is sectional view taken along line D-D. It is shown to the top view of the specimen inspection plate of Example 9. 10 is a top view showing a sample inspection plate of Example 10. FIG.

DESCRIPTION OF SYMBOLS 1 Sample inspection plate 2 Synthetic resin sheet 3 Raised wall 3a Inner raised wall 3b Outer raised wall 4 Sampling part 4a Bottom part 5 Periphery raised frame 6 Grip part 7 Cover 7a Projection part 8 Hinge 9 Recording part 10 Recessed part 11 Identification symbol 12 Recording part 13, 13a, 13b Grip part 14 Folding line

Claims (12)

Sampling portion which is surrounded by at least one raised wall on the synthetic resin sheet is provided at least one,
A sample inspection plate for pathogenic E. coli immune serum agglutination reaction, wherein the bottom of the sample collection part is a flat surface inclined at 1 to 5 degrees .   2. The sample inspection plate according to claim 1, wherein there are a plurality of sample collection portions.   The sample inspection plate according to claim 1 or 2, wherein the sample collection part is rectangular or elliptical. Any one sample testing plate according to Section of the claims 1-3 for the vicinity of the sample collecting part provided with identification symbols. Sample testing plate according to any one of claims 1 to 4 provided a recording unit. Sample testing plate according to any one of claims 1 to 5, sampling portion is surrounded by the inner and outer two raised walls. Sample testing plate according to any one of claims 1 to 6 having provided around a raised frame on the outer circumference. Sample testing plate according to any one of claims 1 to 7 which extend the grip portion. The sample inspection according to any one of claims 1 to 8, wherein the sample collection unit includes eight sample collection units, and identification means is provided between the first sample collection unit and the second sample collection unit. Plate. 10. The sample inspection plate according to claim 9, wherein the means for discriminating between the first sample collection unit and the second sample collection unit has an interval wider than the interval between the second to eighth sample collection units. The sample inspection plate according to any one of claims 1 to 10 , which is formed by vacuum forming. The sample inspection plate according to any one of claims 1 to 10 , which is formed by injection molding.

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