JP2006177875A - Plate for microscopic specimen - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a plate for microscopic specimen which hardly involves air, prevents leak of a liquid sample, is easily handled, and does not contaminate the periphery. <P>SOLUTION: This plate comprises a liquid smear part 4 surrounded by an inside recess 3a and an outside recess 3b, provided on a surface of a glass plate 2. A liquid sample such as a urinary sediment or the like is smeared to the smear part 4. The liquid sample is dammed by the inside recess 3a, and the liquid sample which cannot be dammed by the inside recess 3a is dammed by the outside recess 3b to prevent the leak to the circumference and contamination thereof. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a microscope specimen plate, and more particularly, to a microscope specimen plate used in a clinical examination in which a liquid sample is difficult to leak and air is hardly involved.

The urinalysis is one of the basic and important indexes of clinical examination for grasping individual health conditions, and urinalysis is always performed at the time of health examination and illness. Thus, urinalysis is one of the important indicators of physical condition.
The urine test includes a urine qualitative test for examining urine pH, protein, occult blood reaction, and the like, and a urine sediment test for examining a formed component in urine.
The components of urine sediment are mainly various cylinders, crystals, red blood cells, white blood cells, epithelial cells, and other bacteria derived from the urinary system.

  The sample for urine sediment is prepared by centrifuging the collected urine, removing the supernatant, staining the remaining sediment components, dropping an appropriate amount of the stained sediment components on a slide glass, and then applying a cover glass. Observed.

Regarding such specimens, a plate for preparing a preparation is proposed in which a retention surface surrounded by a frame of a predetermined thickness is formed on a slide glass by screen printing, and a urine sediment staining solution is applied to the retention surface in advance. (For example, refer to Patent Document 1).
JP 7-113731 A

  However, since the plate for preparing a preparation forms a staying surface by screen printing the surrounding frame, the staying surface is formed on the slide glass as a space portion having a minute height by the surrounding frame. Therefore, the staying surface formed in this way is easy to entrain air in the space part, and since the upper and lower directions are open to the staying surface, the urine sediment leaks out from this opening part and contaminates the surroundings. There's a problem.

  On the other hand, there is no practical use of an open part that forms a stay surface that is surrounded by a surrounding frame, but in this case, there is no leakage from the open part as described above. The problem is that it leaks over the surrounding frame and enters the adjacent staying surface, causing contamination and reducing the accuracy of the inspection.

  In view of such a situation, the present invention provides a microscope specimen plate that eliminates the above-described problems of the prior art and is not only difficult to entrain air but also difficult to leak out a liquid sample and is hygienic and excellent in workability. is there.

  A first aspect of the present invention for solving the above-described problems includes a microscope specimen plate characterized in that a liquid sample smear portion surrounded by a concave groove is provided on the surface of a glass plate.

  A second aspect of the present invention includes the microscope specimen plate according to the first aspect, wherein the concave groove is a double concave groove.

  According to a third aspect of the present invention, the inner concave groove has a width of 0.3 to 1.0 mm and a depth of 0.3 to 1.0 mm, the outer concave groove has a width of 0.5 to 1.5 mm, and a depth of 0.00. The microscope specimen plate according to claim 2, comprising 5 to 1.5 mm.

  A fourth aspect of the present invention includes the microscope specimen plate according to any one of the first to third aspects, wherein the liquid sample smear portion is provided at a position slightly lower than the surface of the glass plate.

  A fifth aspect of the present invention is the microscope specimen plate according to any one of the first to fourth aspects, wherein the liquid sample smear portion is provided at a position lower by 0.1 to 0.3 mm than the surface of the glass plate. Content.

  A sixth aspect of the present invention includes the microscope specimen plate according to any one of the first to fifth aspects, wherein a damming portion is provided on a part or all of the outer periphery of the liquid sample smearing portion.

  A seventh aspect of the present invention includes the microscope specimen plate according to any one of the first to sixth aspects, wherein a recording portion is provided in the vicinity of the liquid sample smearing portion.

  Claim 8 of the present invention is for a clinical examination, and includes the plate for a microscope specimen according to any one of claims 1 to 7.

  The microscope specimen plate of the present invention is provided with a liquid sample smearing portion surrounded by a concave groove on the surface of the glass plate, so that it is difficult to entrain air, and an excess liquid sample is accumulated in the concave groove and is blocked. Hygienic and good workability.

  By making the concave groove into double concave grooves, liquid samples that could not be blocked by the inner concave grooves can be blocked by the outer concave grooves, so it is more effective to contaminate the surroundings. To be prevented.

  By providing the liquid sample smear part at a position slightly lower than the surface of the glass plate, preferably at a position lower by about 0.1 to 0.3 mm, it becomes easy to cope with a large amount of smear.

  In addition, by providing a damming part on the whole or part of the outer periphery of the liquid sample smearing part, the liquid sample overflowing from the outermost concave groove is also dammed by the damming part and leaks out of the plate. It is prevented.

  Also, by providing a recording unit in the vicinity of the liquid sample smearing unit, the name, number, symbol, etc. of the liquid sample provider can be described, and the workability and reliability of the inspection can be improved.

  The microscope specimen plate of the present invention is useful for clinical examination, particularly for urine sediment observation.

  The microscope specimen plate of the present invention is characterized in that a liquid sample smear part surrounded by a concave groove is provided on the surface of a glass plate.

  The liquid sample smeared portion may be any of a rectangle, a circle, an ellipse, a polygon, and the like, and the size is not particularly limited, but usually one side in the case of a rectangle and about 8 to 15 mm in the diameter in the case of a circle. Are preferred. If it is smaller than this, it is difficult to smear the liquid sample. On the other hand, if it is larger than this, it is meaningless.

  The concave groove defines at least one liquid sample smearing part and is used to block the liquid sample from leaking out from the smearing part. The upper limit is not particularly limited, but is preferably about 3 (triple), more preferably 2 (double) in terms of practicality.

  The concave groove preferably has a width of about 0.3 to 1.5 mm and a depth of about 0.5 to 1.5 mm. In order to prevent leakage of the liquid sample more reliably, a double structure consisting of an inner concave groove and an outer concave groove, or a triple structure consisting of an inner concave groove, an intermediate concave groove and an outer concave groove. Is preferable. For example, in the case of a double concave groove, the inner concave groove is 0.3 to 1.0 mm wide and 0.3 to 1.0 mm deep, and the outer concave groove is 0.5 to 1.5 mm wide and deep. A thickness of about 0.5 to 1.5 mm is preferable. In order to dam the liquid sample leaking from the inner concave groove with the outer concave groove, it is preferable to design the outer concave groove larger or deeper than the inner concave groove. Even when triple concave grooves are provided, it is preferable to design the inner, middle, outer and concave grooves to be large or deep.

  In order to cope with a liquid sample having a large amount of smear, the liquid sample smearing part is preferably slightly lower than the surface of the glass plate, preferably about 0.1 to 0.3 mm, more preferably 0.1 mm. It is preferable to provide it at a low position. If it is provided at a low position exceeding 0.3 mm, air tends to be engulfed, and, for example, red blood cells tend to move, which tends to be inconvenient.

The glass plate is preferably a rectangle having a side of about 50 to 100 mm from the viewpoint of workability and handleability, and the thickness is preferably about 1 to 3 mm.
The glass plate is provided with at least one liquid sample smearing portion, but it is preferable to provide a plurality of liquid sample spraying portions in view of workability and efficiency, and more preferably five or more. The upper limit is not particularly limited, but if it is too large, the size of the synthetic resin sheet is inevitably increased, and workability and handleability are reduced, so about 20 is preferable.

  By providing a damming portion on a part or all of the outer periphery of the liquid sample smearing part, even if the liquid sample overflows from the outermost concave groove, this liquid sample can be dammed and the plate Leakage to the outside can be prevented. As such a blocking part, for example, a convex streak or a concave groove may be provided on a part or the whole of the outer periphery (entire periphery), or a raised part having a slightly increased overall outer periphery.

By providing a recording unit for recording the name, number, symbol, and the like of the liquid sample provider in the vicinity of the liquid sample smearing unit, workability and requestability can be improved. The recording unit is provided, for example, by roughening the surface or forming a satin finish. In addition, it is possible to preliminarily engrave numbers or symbols in the vicinity of the liquid sample smearing portion of the glass plate at the time of molding, or by printing or the like.
In addition, markings such as changing the roundness (R) of the corners of the glass plate so that the upper and lower sides of the microscope specimen plate can be distinguished, markings, etc. are given to improve the workability of specimen preparation and the reliability of inspection. Is possible.

  The microscope specimen plate of the present invention can be provided with a synthetic resin cover film, if necessary. The cover film has effects such as prevention of evaporation of the liquid sample and dust, and can also serve as a cover glass. Therefore, the cover film needs to have a size sufficient to cover at least the liquid sample smear. The cover film is preferably a synthetic resin film having an adhesive property that adheres closely to the microscope specimen plate and has a moisture barrier property and transparency that does not allow moisture to permeate, and examples of such a film include polyethylene, biaxially oriented polypropylene, and moisture-proof cellophane. , Polyester, polyamide, ethylene-vinyl alcohol copolymer resin, vinyl chloride resin, vinylidene chloride resin, and the like. The thickness of the cover film is not particularly limited, but is preferably about 50 to 300 μm.

The cover film may be detachably bonded to the microscope specimen plate with adhesive or adhesive at both ends of the cover film. The cover film may be hinged with a seal, an adhesive, or an adhesive, and the other end of the cover film may be freely opened and closed. In this case, when it is desired to laminate the adhesive or pressure-sensitive adhesive and release paper in this order, and to bond or temporarily bond them, the release paper can be peeled off and bonded or temporarily bonded with the exposed adhesive or pressure-sensitive adhesive.
Further, the cover film can be easily opened and closed by notching a part of the end portion of the glass plate so that the cover film is made only as a cover film and the cover film is easily picked up with a finger.

  In the microscope specimen plate of the present invention, a cover plate made of glass or synthetic resin and having a certain degree of rigidity can be used instead of the film. The glass that is the material of the cover plate may be a glass that is usually used as a cover glass, and the synthetic resin may be a synthetic resin listed as a material for the cover film.

  The microscope specimen plate of the present invention as described above is useful for clinical examinations, particularly for microscopic examination of liquid samples such as urine sediment, saliva, blood and the like, but is particularly suitable for microscopic examination of urine sediment.

  Hereinafter, the present invention will be described in more detail with reference to the drawings illustrating examples. However, the present invention is not limited to the examples.

Example 1
1 to 3 show the present embodiment. FIG. 1 is a top view of a microscope specimen plate, FIG. 2 is a cross-sectional view taken along line AA, and FIG. 3 is a liquid sample smear surrounded by a concave groove. It is an enlarged view.
In these figures, the microscope specimen plate 1 has five liquid sample smears 4 formed on the surface of a glass plate 2 and surrounded by a concave groove 3 in each of an upper stage and a lower stage.

The glass plate 2 is 50 mm × 85 mm × 2 mm, the inner concave groove 3 a made of a concave groove has a width of 0.5 mm, a depth of 0.5 mm, an inner side of 8 mm, and the outer concave groove 3 b made of a concave groove has a width of 1 mm and a depth. The liquid sample smear part (8 mm × 8 mm) 4 is formed of 1 mm and an inner side of 11 mm and surrounded by the inner concave groove 3a and the outer concave groove 3b.
The upper left corner 2a of the glass plate 2 is designed to have a larger radius than the other three corners. This is because the upper and lower sides of the microscope specimen plate 1 are easily distinguished, and the workability of specimen preparation is improved. And to improve the reliability of the inspection.

  In order to prepare a microscope specimen using the above-described microscope specimen plate 1, urine is collected and centrifuged to remove the supernatant, and the remaining sediment is smeared on the liquid sample smearing part 4 of the plate 1. After staining with a staining reagent, usually, as described below, a cover film or a cover plate is attached to form a specimen, which is then subjected to microscopic examination.

Example 2
As shown in FIGS. 4 and 5, the microscope specimen plate 1 of this embodiment has a cover film 5 attached to the surface of a glass plate 2. One end (left end portion in the figure) of the cover film 5 is bonded to the glass plate 2 by a heat seal 6.
In FIG. 4, a notch 7 is provided at substantially the center of the right end portion of the glass plate 2, and this is because when the cover film 5 is opened and closed, the portion of the notch 7 is only the cover film 5. Therefore, the cover film 5 is designed so that it can be easily opened and closed with a finger. Further, by bonding the left end portion of the cover film 5 with a heat seal or an adhesive, the bonding portion 7 serves as a hinge, and the cover film 5 can be easily opened and closed.

Example 3
As shown in FIG. 6, the microscope specimen plate 1 of the present embodiment is provided with a recording portion 8 made of a satin finish on the upper part of the liquid sample smearing portion 4 surrounded by the inner concave groove 3 a and the outer concave groove 3 b. Is the same as in the second embodiment.
Thus, by providing the recording unit 8, the name, number, symbol, and the like of the urine sediment provider can be recorded, so that workability and reliability are improved.

Example 4
As shown in FIG. 7, the microscope specimen plate 1 of this example is provided with a number 10 in the vicinity of the liquid sample smearing portion 4 of the glass plate 2 (in this example, the upper part is the upper part and the lower part is the lower part), The cover film 5 is adhered to the glass plate 2 with an adhesive (adhesive) instead of heat sealing at the left end, and the same as in Example 2 except that the adhesive portion 9 is a hinge portion.

Example 5
As shown in FIG. 8, the microscope specimen plate 1 of this embodiment is provided with a number 10 in the vicinity of the liquid sample smearing portion 4 of the glass plate 2 (in this example, the upper part is the upper part and the lower part is the lower part). Is the same as in the second embodiment. In the present example, unlike the case of the fourth embodiment, the numbers are assigned from the left to the right in the upper and lower stages.

Example 6
As shown in FIG. 9, the microscope specimen plate 1 of this example is detachably adhered to the surface of the glass plate 2 by temporarily (weakly) adhering the cover film 5 with an adhesive near the left and right ends. Others are the same as in the second embodiment. In the figure, reference numerals 11a and 11b denote adhesive portions.
In this case, it is good also as a hinge part using a heat seal or an adhesive agent like Example 2 instead of the adhesion part 11b.

Example 7
As shown in FIG. 10, the microscope specimen plate 1 of this example has a depth of the inner concave groove 3 a of 0.4 mm, a depth of the outer concave groove 3 b of 0.7 mm, and the liquid sample smearing portion 4. Example 1 is the same as Example 1 except that it is provided at a position 0.1 mm lower than the surface of the glass plate 2. The microscope specimen plate 1 of this embodiment is suitable for a liquid sample with a large amount of smear.

Example 8
As shown in FIGS. 11 and 12, the microscope specimen plate 1 of the present embodiment changes the vertical and horizontal ratios of the glass plate 2, makes the liquid sample smear part 4 rectangular, and the liquid sample smear part 4. No. 10 is provided in the vicinity of, and a dam portion 12 made of a concave groove is provided on the right side of the plate 1 in the same manner as in the first embodiment. Even if the liquid sample leaks out of the outer concave groove 3b, the plate 1 is tilted to the right side to accumulate in the concave groove of the damming portion 12, and leakage out of the plate is prevented.

Example 9
As shown in FIGS. 13 and 14, the microscope specimen plate 1 of this example is the same as that of Example 8 except that a damming portion 13 a formed of a concave groove is provided along the periphery of the plate 1. It is. Even if the liquid sample leaks out of the outer concave groove 3b, the plate 1 is inclined to the left, right, up, down or up, so that it accumulates in the concave groove of the damming portion 13a and is prevented from leaking out of the plate. Is larger than the plate of Example 8 in which the blocking portion 12 is partially provided.

Example 10
As shown in FIGS. 15 and 16, the microscope specimen plate 1 of this example is the same as that of Example 8 except that the number of liquid sample smearing parts 4 is five.

Example 11
As shown in FIGS. 17 and 18, the microscope specimen plate 1 of this example is the same as that of Example 9 except that the number of liquid sample smearing parts 4 is five.

Example 12
As shown in FIGS. 19 and 20, the microscope specimen plate 1 of the present embodiment is the same as that of the ninth embodiment except that the damming portion 13a made of a concave groove is a damming portion 13b made of a convex line. It is. Even if the liquid sample leaks out from the outer concave groove 3b, the liquid sample is blocked by the blocking portion 13b made of convex streaks, and leakage out of the plate is prevented.

Example 13
As shown in FIGS. 21 and 22, the microscope specimen plate 1 of the present embodiment is the same as that of the ninth embodiment except that the damming portion 13a made of a concave groove is replaced with a damming portion 13c made of a raised portion. is there. Even if the liquid sample leaks out of the outer concave groove 3b, the liquid sample is blocked by the blocking portion 13c formed of a raised portion, and leakage outside the plate is prevented.

Example 14
As shown in FIG. 23, the microscope specimen plate 1 of this example is the same as that of Example 8 except that a cover plate 14 is attached so as to cover at least the liquid sample smearing part.

Example 15
As shown in FIG. 24, the microscope specimen plate 1 of the present embodiment is the same as that of the ninth embodiment except that a cover plate 14 is attached so as to cover at least the liquid sample smear portion.

Example 16
As shown in FIG. 25, the microscope specimen plate 1 of the present example is the same as that of Example 10 except that a cover plate 14 is attached so as to cover at least the liquid sample smear part.

Example 17
As shown in FIG. 26, the microscope specimen plate 1 of this example is the same as that of Example 11 except that the cover plate 14 is attached so as to cover at least the liquid sample smearing part.

  As described above, the microscope specimen plate of the present invention is provided with a liquid smear part surrounded by a concave groove, so that it is difficult to entrain air and can effectively prevent leakage of a liquid sample. Easy to handle and hardly contaminates the surroundings.

2 is a top view showing a microscope specimen plate of Example 1. FIG. It is AA sectional drawing of FIG. It is an enlarged view of the liquid sample smear part enclosed by the inner side concave groove and the outer side concave groove. 6 is a top view showing a microscope specimen plate of Example 2. FIG. It is the BB sectional drawing. 6 is a top view showing a microscope specimen plate of Example 3. FIG. 6 is a top view showing a microscope specimen plate of Example 4. FIG. 10 is a top view showing a microscope specimen plate of Example 5. FIG. 6 is a top view showing a microscope specimen plate of Example 6. FIG. FIG. 10 is a cross-sectional view of a main part showing a microscope specimen plate of Example 7. 10 is a top view showing a microscope specimen plate of Example 8. FIG. It is CC sectional drawing. 10 is a top view showing a microscope specimen plate of Example 9. FIG. It is the DD sectional drawing. 10 is a top view showing a microscope specimen plate of Example 10. FIG. It is EE sectional drawing. 10 is a top view showing a microscope specimen plate of Example 11. FIG. It is the FF sectional drawing. 10 is a top view showing a microscope specimen plate of Example 12. FIG. It is the same GG sectional view. 14 is a top view showing a microscope specimen plate of Example 13. FIG. It is the HH sectional view. 16 is a top view showing a microscope specimen plate of Example 14. FIG. FIG. 16 is a top view showing a microscope specimen plate of Example 15; 18 is a top view showing a microscope specimen plate of Example 16. FIG. 18 is a top view showing a microscope specimen plate of Example 17. FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Microscope specimen plate 2 Glass plate 3a Inner concave groove 3b Outer concave groove 4 Liquid sample smear part 5 Cover film 6 Heat seal 7 Notch 8 Recording part 9 Adhesion part 10 Number 11a, 11b Adhesive part 12 Weir part (concave part) groove)
13a Damping part (concave groove)
13b Damping part (convex streak)
13c Damping part (raised part)
14 Cover plate

Claims (8)

  A microscope specimen plate, characterized in that a liquid sample smear part surrounded by a concave groove is provided on the surface of a glass plate.   2. The microscope specimen plate according to claim 1, wherein the concave groove is a double concave groove.   The inner concave groove has a width of 0.3 to 1.0 mm and a depth of 0.3 to 1.0 mm, and the outer concave groove has a width of 0.5 to 1.5 mm and a depth of 0.5 to 1.5 mm. Item 3. The microscope specimen plate according to Item 2.   The plate for microscope specimens of any one of Claims 1-3 which provided the liquid sample smearing part in the position slightly lower than the surface of a glass plate.   The plate for microscope specimens of any one of Claims 1-4 which provided the liquid sample smear part in the position 0.1-0.3 mm lower than the surface of a glass plate.   The microscope specimen plate according to any one of claims 1 to 5, wherein a damming portion is provided on a part or all of the outer periphery of the liquid sample smearing portion.   The plate for a microscope specimen according to any one of claims 1 to 6, wherein a recording unit is provided in the vicinity of the liquid sample smearing unit.   The plate for a microscope specimen according to any one of claims 1 to 7, which is for clinical examination.

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