JP2012032589A - Preparation for inspection of liquid sample - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a preparation for inspection of a liquid sample capable of easily storing a sample and performing microscopic inspection irrespective of difference in viscosity.SOLUTION: A preparation 1 for inspection of a liquid sample comprises: a substrate part 2; and a lid part 3. A recessed cavity part 2a for storing a sample is provided on the bottom surface of the substrate part 2 and the lid part 3 is provided integrally with the substrate part 2 through a hinge part 3a and can be coated on the recessed cavity part 2a. A notch part 3b, which is an opening for sample injection in coating the lid part 3 on the recessed cavity part 2a, is provided on the lid part 3. The depth of the recessed cavity part 2a is a depth in which a capillary phenomenon occurs between the lower surface of the lid part 3 and the bottom surface of the recessed cavity part 2a in a state of coating the lid part 3 on the recessed cavity part.

Description

  The present invention relates to a preparation for inspecting a liquid sample such as a body fluid with an optical microscope, and in particular, for a liquid sample inspection that can easily and uniformly accommodate a sample regardless of the level of viscosity. Regarding preparations.

In the case of observing a liquid sample with a microscope, a preparation is conventionally prepared by dropping a few drops of the liquid sample on a rectangular glass plate called a slide glass, performing a predetermined process such as staining, and covering with a cover glass. It was.
However, it is not easy to make a liquid sample to a certain thickness, so that preparation of a preparation requires considerable skill, and the liquid sample leaks between the slide glass and the cover glass to contaminate the fingers. There was also. Further, since both the slide glass and the cover glass are made of glass, there is a problem that they are heavy and easily broken.

  In order to eliminate such drawbacks, the plastic slide glass and the cover glass are closely attached via a slight gap, and the partition wall seals the right and left of the gap (cell), and the opening for sample injection before and after the gap. A slide (see Patent Document 1) provided with a portion and a vent is proposed. In this slide, if a liquid sample is dropped into the opening for sample injection, the sample is automatically drawn into the cell by capillary action and spreads, and the thickness of the sample becomes uniform, so that a preparation can be easily made.

JP-A-4-214519

However, not only samples with low viscosity but also samples with high viscosity such as urine sediments are to be examined. Even if such a highly viscous sample is dropped into the opening of the slide described in Patent Document 1, the sample is not automatically sucked due to capillary action, and the sample is stored in the cell. Impossible.
In view of such circumstances, the present invention solves the above-mentioned problems of the prior art, and prepares a specimen for liquid sample inspection that can easily accommodate a sample and create a preparation not only for a sample having a low viscosity but also for a sample having a high viscosity. The purpose is to provide.

  In order to achieve the above object, a first feature of the present invention is that it includes a substrate portion and a lid portion, and the substrate portion is provided with a concave portion for receiving a sample, and the lid portion is a hinge. Is provided integrally with the substrate portion via the portion, and can cover the concave portion, and the lid portion includes an opening for sample injection when the concave portion is covered with the lid portion. The depth of the concave portion is such that a capillary phenomenon occurs between the lower surface of the lid portion and the bottom surface of the concave portion with the lid portion covered on the concave portion. The preparation for liquid sample inspection is the depth.

  The second feature of the present invention is the above-described preparation for liquid sample inspection, in which a groove deeper than the recess is provided around the recess.

  A third feature of the present invention is the liquid sample inspection preparation as described above, wherein a wall portion is provided on all or part of the inside of the groove portion.

  According to a fourth aspect of the present invention, a finger insertion recess is provided in the vicinity of the recessed portion at the edge of the substrate portion, a finger hooking projection is provided at the edge of the lid portion, and the cover portion is formed in the recessed portion. The above-mentioned preparation for liquid sample inspection is such that the finger-hanging projection protrudes into the finger-insertion recess when coated.

  A fifth feature of the present invention is that the liquid sample is provided with a locking structure for fixing the lid portion to the substrate portion between the finger hooking projection of the lid portion and the finger insertion recess of the substrate portion. The preparation preparation is the content.

  A sixth feature of the present invention is the above-described preparation for liquid sample inspection in which the surface of the substrate portion excluding the concave portion is roughened.

The preparation for liquid sample inspection according to the present invention is provided with a notch portion serving as an opening for sample injection at the edge of the lid portion, and in a state where the lid portion is covered on the concave portion that is a sample storage portion. Since the gap between the bottom surface of the lid and the bottom surface of the recess is configured so that capillary action occurs, in the case of a sample with low viscosity, the lid is covered on the recess for sample injection. When the sample is dropped into the opening, the sample automatically spreads between the lid and the recess due to capillary action, and the sample is accommodated in the recess.
When the viscosity of the sample is high, the sample can be easily accommodated in the concave portion by lifting the lid.

  Since the groove portion deeper than the concave portion is provided around the concave portion, an extra sample is accommodated in the groove portion and is prevented from leaking out and being contaminated.

  By providing a wall on the whole or part of the inside of the groove, the sample dripped into the opening is blocked by the wall, and after the sample is filled in the recess, the excess sample overflows into the groove. Therefore, troubles such as shortage of sample or bubbles remaining in the sample in the recess are prevented, and when the wall is provided in part, the absence of the wall functions as an air vent, Troubles in which air remains in the sample can be prevented more effectively.

  When the finger insertion recess is provided in the vicinity of the recessed portion at the edge of the substrate portion, the finger hooking projection is provided on the edge of the lid portion, and the cover portion is covered with the recessed portion, By projecting in the finger insertion recess, the lid can be freely opened and closed by placing a finger on the finger hooking projection, and the finger hooking projection fits within the outer shape of the substrate portion and does not protrude. Troubles that obstruct the microscope observation or that the lid part unexpectedly opens when hitting an instrument or the like are prevented.

  In the case of providing the finger hooking protrusion and the finger insertion recess, by providing a locking structure for fixing the lid portion to the substrate portion, there is no possibility that the lid portion opens unexpectedly and the sample is not spoiled.

  If the surface of the substrate portion excluding the concave portion is roughened, it is possible to easily distinguish between the place where the sample is stored and the other place at the time of microscopic observation, thereby improving workability. In addition, the name and symbol of the subject can be written or printed.

FIG. 1A is a schematic view showing an example of a preparation for liquid sample inspection of the present invention, FIG. 1B is a cross-sectional view taken along the line AA in FIG. 1A, and FIG. It is sectional drawing. FIG. 2A is a schematic view showing a state in which the lid portion is covered with the concave portion in FIG. 1, and FIG. 2B is a cross-sectional view taken along the line CC in FIG. FIG. 3 is an enlarged cross-sectional view in a state where the lid portion is covered with the concave portion in FIG. FIG. 4 is a schematic view of a preparation in which the surface of the substrate portion excluding the concave portion is roughened. FIG. 5 is a schematic view showing another example of the preparation for liquid sample inspection of the present invention. FIG. 6 is a schematic view showing a state in which the lid portion is covered with the concave portion in FIG. FIG. 7 is a schematic view showing another example of the preparation for liquid sample inspection of the present invention. FIG. 8 is a schematic view showing another example of the preparation for liquid sample inspection of the present invention.

  The liquid sample inspection preparation 1 of the present invention comprises a substrate portion 2 and a lid portion 3 as shown in FIGS. 1 to 3, and the substrate portion 2 is provided with a concave portion 2a for accommodating a sample. The lid portion 3 is provided integrally with the substrate portion 2 via a hinge portion 3a, and can be covered with the concave portion 2a. The lid portion 3 includes the lid portion 3 and the concave portion. A notch 3b is provided as an opening for sample injection when the part 2a is covered, and the depth of the concave part 2a is such that the lid part 3 is covered on the concave part 2a. The depth is such that capillary action occurs between the lower surface of the portion 3 and the bottom surface of the recessed portion 2a.

As shown in FIG. 1, the preparation for liquid sample inspection 1 of the present invention comprises a substrate portion 2 and a lid portion 3. The lid portion 3 is connected to the substrate portion 2 via a hinge portion 3a.
The shape of the substrate part 2 is not particularly limited, but it is preferable to use a horizontally long rectangular shape like a slide glass that has been frequently used for microscope observation, since it can be set on a microscope in the same manner as in the past and the sample can be observed.

  The substrate portion 2 is provided with a concave portion 2a for accommodating a sample, and the concave portion 2a is covered with the lid portion 3 as shown in FIG. The shape of the lid portion 3 and the concave portion 2a is not particularly limited as long as the concave portion 2a can be covered with the lid portion 3 except for an opening for sample injection which will be described later. Similarly, if a square shape with a side of about 15 to 20 mm is used, the appearance will be the same as when a normal slide glass and cover glass are used, so that the inspection can be performed in the same manner as before.

The hinge portion 3a in the present invention is a portion for allowing the lid portion 3 to be opened and closed on the concave portion 2a. Although the structure is not particularly limited, a preparation for specimen inspection is often handled as a disposable, and it is sufficient if it has a strength enough to withstand several uses.
For example, when the lid portion 3 and the substrate portion 2 are formed, the space between the two portions can be a thin film, which can be used as the hinge portion 3a. If the substrate part 2 and the cover part 3 are connected by such a hinge part 3a, depending on the material, the cover part 3 will not break even if it is opened and closed as many times as required for the preparation (about 2 to 3 times). This structure can be manufactured at low cost. Moreover, it can replace with a thin film form and can make a notch (notch) on the back side, and can also be set as a hinge part.

  In the present invention, if the concave portion 2a is covered with the lid portion 3, a space surrounded by the concave portion 2a and the lid portion 3 is formed as shown in FIG. 3, and this space (hereinafter referred to as the sample chamber 5) is formed. In some cases, the sample is injected and contained. The shape of the sample chamber 5 may be determined arbitrarily so that the sample can be easily observed according to the sample and the content of the inspection, but is usually about 15 to 20 mm in length and width and about 0.2 to 0.5 mm in height. .

In the present invention, the lid portion 3 is provided with a notch 3 b serving as an opening for dropping a liquid sample into the sample chamber 5. More specifically, as shown in FIG. 2, if the concave portion 2a is covered with the lid portion 3 provided with the notch portion 3b as shown in FIG. 1, the notch portion 3b is formed as shown in FIG. When the sample is dropped into this opening for sample injection, the sample is injected and accommodated in the sample chamber 5 by capillary action between the lid 3 and the recess 2a.
The position of the cutout portion 3b is not particularly limited as long as it is a portion that overlaps the concave portion 2a when the lid portion 3 is put on the concave portion 2a, but the concave portion 2a does not interfere with sample observation with a microscope. It is preferable to provide the notch portion 3b at a position overlapping with the end portion. In the example shown in FIGS. 1 to 3, a notch 3b is provided on the hinge 3a side, and a part of the notch 3b is formed when the lid 3 is covered with the recess 2a. It is configured to overlap with the end of the recessed portion 2a to be an opening for sample injection.

  As described above, in the present invention, when a liquid sample is dropped into the sample injection opening 3b, the liquid sample is spread and accommodated in the sample chamber 5 by capillary action. Specifically, for example, by setting the distance between the lower surface of the lid 3 and the bottom surface of the concave portion 2a (that is, the height of the sample chamber 5) to about 0.2 to 0.5 mm, urine, blood, A liquid sample having a relatively low viscosity such as tissue fluid is automatically injected and stored in the sample chamber 5 by capillary action.

  In the present invention, as shown in FIG. 1, a groove 2b deeper than the recess 2a is provided around the recess 2a. As a result, an extra sample that does not fully enter the sample chamber 5 flows into the groove 2b, so that the risk of the extra sample leaking out from the sample injection opening 3b and contaminating the surface of the substrate portion 2 is reduced. Further, since it is not necessary to worry about the leakage of the sample, it is possible to prevent a trouble that the amount of the dropped sample is too small and a sufficient sample is not injected into the sample chamber 5.

  The liquid sample testing preparation 1 of the present invention is often used for testing a sample with low viscosity. For example, when the sample has a high viscosity, such as urine sediment, the sample is dropped as described above. It is difficult to inject and accommodate in the sample chamber 5 due to capillary action. In such a case, the lid 3 is lifted and the sample is directly stored in the concave portion 2a, and then the lid 3 is covered on the concave portion 2a. In this case, if necessary, the sample is spread from the top of the lid 3.

As a method for making the lid 3 easy to lift, for example, as shown in FIG. 1, a finger hooking projection 3 c can be provided at the tip of the lid 3, and the lid 3 can be opened by hooking a finger on this portion. The method of doing is mentioned. However, if the finger hooking projection 3c protrudes from the edge of the substrate portion 2, there is a greater risk that the inspection device or the like will hit the finger hooking projection 3c during inspection and the lid portion 3 will open unexpectedly. It is difficult to lift the lid 3 with a finger.
Therefore, as shown in FIG. 2, the finger insertion recess 2c is provided in the vicinity of the recess 2a on the edge of the substrate portion 2, and the finger hooking projection 3c protrudes in the finger insertion recess 2c. It is preferable to configure so as not to protrude within the outer shape of the substrate portion 2. In this way, it is possible to place a finger on the finger hooking protrusion 3c, and the risk that the inspection instrument or the like hits the finger hooking protrusion 3c and the lid portion 3 opens unexpectedly is reduced.

  In order to prevent the lid portion 3 from opening unexpectedly, it is preferable to provide a locking structure 4. Although a specific structure is not particularly limited, for example, as shown in FIG. 3, the protrusion 4 a is provided from the tip of the finger hooking protrusion 3 c of the lid portion 3 toward the inside, and the finger insertion recess 2 c of the substrate portion 2. There is a structure in which the protrusions 4b are provided from the inside to the outside, and these protrusions 4a and 4b are engaged with each other. When the lid 3 is opened, the engagement between the protrusions 4a and 4b can be easily released by placing a finger on the finger hooking protrusion 3c and bending it slightly upward.

As shown in FIG. 4, the substrate portion 2 can be distinguished from the concave portion 2 and other places at a glance by roughening the surface excluding the concave portion 2, for example, By simply looking through the microscope, it is possible to immediately determine whether or not the concave portion 2 is under the objective lens, and workability is improved.
In addition, by roughening the surface of the substrate part 2, the name and number of the subject can be written or printed as necessary to prevent troubles such as mistaking the subject. Can do.

  In the above example, as shown in FIG. 1, the example in which the groove portion 2b is provided around the entire periphery of the concave portion 2a has been shown. However, as shown in FIGS. The groove 2b can be omitted in the vicinity of the cutout 3b. This makes it difficult for the sample to flow into the groove 2b, so that even when the amount of the sample to be dropped is small, the inspection can be performed without problems. Further, it is possible to prevent a trouble that the sample flows into the entire groove 2b first and bubbles remain in the center.

Moreover, in order to prevent a sample from flowing into the groove part 2b previously, as shown in FIG. 7, the wall part 2d can also be provided inside the groove part 2b. In this way, the sample dripped into the opening is blocked by the wall 2d, and after the recess 2a is filled with the sample first, the excess sample overflows into the groove 2b. Is further prevented from becoming insufficient or bubbles remaining in the sample in the concave portion 2a.
Further, the wall 2d may be provided on the entire inside of the groove 2b as shown in FIG. 7, but as shown in FIG. 8, the wall 2d faces the side where the opening for dropping the sample is provided. If the wall portion 2d is not provided in a part of the side and the absence portion of the wall portion 2d is vented, the trouble that air bubbles remain in the sample in the concave portion 2a can be more effectively prevented.

As shown in FIGS. 2 and 6, when a plurality of concave portions 2a are provided, identification symbols for distinguishing the concave portions 2a (in the drawings, indicated as A, B, and C). Is preferred). The identification symbol that can be used is not limited to the alphabet as shown, but may be anything that can distinguish the concave portions 2a such as hiragana, katakana, numerals, and geometric patterns.
The place to which the identification symbol is attached is not particularly limited as long as each recessed portion 2a can be distinguished, and the identification symbol may be attached to the finger hooking projection 3c as shown in the figure, and the circumference of each recessed portion 2a, for example, each recessed portion You may attach | subject between the pair of hinge parts 3a of the cavity 2a.
The method of attaching the identification symbol is not particularly limited, and for example, the identification symbol may be engraved on a mold for forming the preparation 1 of the present invention so that the identification symbol protrudes in a relief shape. An identification symbol may be printed after molding.

  The material of the liquid sample inspection preparation 1 in the present invention is not particularly limited as long as it is a transparent polymer material, and can be selected from ordinary optical transparent resins. Specific examples thereof include acrylic resins, polycarbonate, polystyrene, polyvinyl chloride, polypropylene, polymethylpentene and other polyolefins, cyclic polyolefins, polyethylene terephthalate, polybutylene terephthalate, polyethylene naphthalate and other polyester resins, polyamide resins, poly An arylate, a polyimide, etc. can be mentioned, Among these, an acrylic resin, a polycarbonate, and cyclic polyolefin are especially preferable.

  As described above, according to the preparation for liquid sample inspection of the present invention, a preparation can be prepared very easily not only when the viscosity of the liquid sample is low but also when the viscosity is high, and its usefulness is greatly increased.

DESCRIPTION OF SYMBOLS 1 Preparation for liquid sample inspection 2 Substrate part 2a Recessed part 2b Groove part 2c Finger insertion recessed part 2d Wall part 3 Lid part 3a Hinge part 3b Notch part (opening part for sample injection)
3c Finger-hanging projection 4 Locking structure 4a, 4b Projection 5 Sample chamber

Claims (6)

It consists of a substrate part and a lid part,
The substrate portion is provided with a recess for receiving a sample on the bottom surface,
The lid portion is provided integrally with the substrate portion via a hinge portion, and can cover the concave portion.
The lid part is provided with a notch part that becomes an opening for sample injection when the concave part is covered with the lid part,
The depth of the concave portion is a depth at which capillary action occurs between the lower surface of the lid portion and the bottom surface of the concave portion with the lid portion covered on the concave portion. Preparation for liquid sample inspection.   The preparation for liquid sample inspection according to claim 1, wherein a groove portion deeper than the recessed portion is provided around the recessed portion.   The liquid sample inspection preparation according to claim 2, wherein a wall portion is provided on all or part of the inside of the groove portion.   A finger insertion recess is provided in the vicinity of the recessed portion at the edge of the base plate portion, and a finger hooking projection is provided on the edge of the lid portion. The preparation for a liquid sample inspection according to any one of claims 1 to 3, wherein is in a state of protruding in the finger insertion recess.   The liquid sample according to claim 4, wherein a locking structure is provided between the finger hooking protrusion of the lid portion and the finger insertion concave portion of the substrate portion to fix the lid portion to the substrate portion. Preparation for inspection.   6. The preparation for liquid sample inspection according to claim 1, wherein the surface of the substrate portion excluding the concave portion is roughened.

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