JPH08334702A - Plastic slide for optical microscope - Google Patents

Abstract

PURPOSE: To provide a slide for optical microscope with which thicknesses of the liquid phases of samples are held constant regardless the kinds and quantities of the samples and which has no fair that the samples are adhered to human body at the time of handling. CONSTITUTION: Cells 5 for holding samples are formed with a base 1, a cover 2, struts 15 and partitions 3 and the base 1 and the cover 2 are made of transparent plastic and the partition 3 seal the lefts and rights of cells 5 hermetically and also have a function holding the gap between the base 1 and the cover 2 constant together with the struts 15 and this slide has opening parts for injecting the samples at front parts of cells 5 and minute air passing holes through which air can permeate but liquid do not flow at rear parts of the cells 5.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a slide for an optical microscope. The optical microscope slide of the present invention allows the sample thickness to be kept constant by a simple operation without touching the human body with the sample, and thus allows safe and simple microscopy.

[0002]

2. Description of the Related Art As a base and a cover of a slide for an optical microscope, those made of glass are conventionally used.
Since it is difficult to process the surface of glass, the surfaces of the base and cover were almost flat. Therefore, when observing a liquid sample with an optical microscope,
A sample is dropped on glass, and the sample is covered with a cover glass for observation, but the thickness of the liquid phase of the sample changes each time, and thus a complicated operation was required to keep this constant.
Further, since the sample adheres to the periphery of the cover glass, it was difficult to avoid the sample adhering to the fingertip. In recent years, with the development of plastics, plastic bases and covers have come to be made, and those in which a base and a cover are integrally molded are commercially available. In conventional monolithic slides for optical microscopes, the thickness of the liquid phase is constant, but the thickness is extremely thick at 80-100 μm, making it unsuitable for high-magnification observation. It has the drawback that it easily leaks and easily adheres to the hands of the inspector. Especially in recent medical examinations, because there is a possibility that harmful viruses such as hepatitis virus may be mixed in the sample, slides
It is an important issue to prevent the sample leaking from the glass from adhering to the microscopic examiner and other human bodies. In order to solve such problems, cell for sample holding is based,
There has been proposed a plastic slide for an optical microscope, which is formed of a cover and a partition, and has an opening for sample injection in the front part of the cell and a gas flow port in the rear part of the cell (see JP-A-4-214519).

[0003]

The plastic slide for an optical microscope described above is capable of keeping the thickness of the sample constant, and there is no fear of the sample coming into contact with the human body, but when a sample having a large surface tension is injected. If the injection volume of the sample is less than the capacity of the cell, the cover will bend due to surface tension,
There was a problem that the microscopic examination might be hindered.
Therefore, an object of the present invention is to provide a slide for an optical microscope, which keeps the thickness of the liquid phase of the sample constant regardless of the type and amount of the sample, and does not cause the sample to adhere to the human body during handling. is there.

[0004]

According to the present invention, there are provided the following objects: (1) A cell for holding a sample is formed by a base, a cover, a support and a partition, and the base and the cover are made of transparent plastic. The partition wall has a function of sealing the left and right sides of the cell and maintaining a constant distance between the base and the cover together with the support.A sample injection opening is formed in the front portion of the cell, and gas is passed through the rear portion of the cell. A slide for an optical microscope characterized by having a minute gas flow port through which a liquid does not flow, (2) a base, a cover, a support and a partition are integrally made of plastic to form a cell for holding a sample. The length of the cover in the front-rear direction is shorter than that of the base, and the left and right sides of the cell are closed by partition walls between the base and the cover, and grooves are formed on the base along the front and rear edges of the cover. The gap between the front part of the cover and the cover serves as a sample injection port, the gap between the rear part of the base and the cover serves as a gas flow port, and a cell for holding the sample is defined by the left and right partitions and the front and rear grooves. A slide for an optical microscope characterized by having (3) a base, a cover,
The pillar and partition are made of plastic integrally, and the cell that holds the sample is formed by them.The length of the cover in the front-back direction is shorter than the base, and the space between the base and the cover is blocked by the partition on the left and right of the cell. , The front and rear parts of the base are provided with a tapered portion in which the distance between the base and the cover is gradually widened toward the outside, and a groove is formed on the base along the edges of the front and rear parts of the cover on the outside of the tapered portion. Is provided, the gap between the base and the tip of the cover serves as a gas flow port, and the left and right partition walls and the front and rear taper portions form a sample holding cell having a defined predetermined interval, and further the front groove. The slide for an optical microscope, which has a sample injection port that communicates with the front and rear edges of the base, and (4) the cell-side surface of the base (contacting the sample). It is achieved by providing a slide for an optical microscope according to the above (1) to (3), characterized in that a convex graduation line is provided on the surface).

In the present invention, the base and cover forming the cell for holding the sample must be made of plastic, and the partition walls and columns forming the cell are also preferably made of plastic. A wide range of materials are available for the type of plastic, for example:
Thermoplastic such as acrylic resin such as polymethylmethacrylate, polystyrene resin, polycarbonate resin, polysulfone resin, polyester resin, nylon resin, ethylene acrylic resin, polyethylene resin, polypropylene resin, poly-4-methylpentene-1 resin, polyvinyl chloride resin, etc. Examples of the plastic include thermosetting plastics such as phenol resin and furan resin, and these may be appropriately mixed and used. Since some of these plastics contain a plasticizer, the material should be selected in consideration of the properties of the sample, and in the case of heat sterilization, the heat resistance of the plastic should also be considered. Is good. For example, when sterilization is required, a plastic having high heat resistance such as polycarbonate resin, polysulfone resin, poly-4-methylpentene-1 resin is preferable. It is necessary that the plastic used for the base and the cover be transparent, and it is preferable that the plastic has high optical isotropy, especially when observed at a high magnification of 1000 times or more. In general, many thermoplastics have crystallinity and exhibit birefringence by stretching, so it is necessary to take care not to cause birefringence during molding.

In recent years, with the development of plastic lenses, plastics having high transparency and high optical isotropy have been developed, and those having further improved light transmission by a surface coating treatment are used. Acrylic resin is mainly used as a material for these optical plastics. These are also extremely excellent as materials for the slide for an optical microscope of the present invention. The base and the cover may be made of the same plastic material or different plastic materials. The material of the partition walls and the columns is not particularly limited, but it is preferable to use plastic to integrally form the base or the cover because it is easy to keep the distance between the base and the cover constant. Also, an adhesive strip or adhesive may be applied to the partition. The shape and structure of the sample inlet at the front of the cell are not particularly limited. The sample is mainly a liquid, but a wide range can be used as long as the cell can be filled.

In the present invention, it is necessary to provide at least one pillar inside the cell in order to prevent the cover from being curved. If the number of the columns is too large, it will hinder the microscopic examination, so that the number of the columns is preferably 3 or less. As the place to install the pillar, if there is one pillar, near the center of the cell, 2
In the case of one, near the sample inlet and near the gas flow port, 3
In the case of individual cells, the vicinity of the center of the cell, the vicinity of the sample injection port and the vicinity of the gas flow port are preferable. The shape of the column is not particularly limited, and examples thereof include a columnar shape, a conical shape, a truncated cone shape, and a hemispherical shape. The size of the column is not particularly limited, but from the viewpoint of not hindering the field of view during a microscopic examination, if the column has a columnar shape, a conical shape, a truncated cone shape, a hemispherical shape, or the like, the diameter is 0.5. It is preferably less than or equal to mm.

The optical microscope slide of the present invention is mainly used for microscopic examination of liquids, and most frequently used for examination of blood, cells, urine, etc. in medical institutions, such as water, physiological saline, ethylene glycol, It is often used after diluting with a medium used in the medical field such as dimethyl sulfoxide. For this reason, a minute gas flow port through which gas flows but liquid does not flow is provided at the rear of the cell. Therefore, when the sample is injected, the air inside the cell is released to the outside through the gas flow port, the inside of the cell is replaced with the sample, and the leakage of the sample to the outside is prevented. The gap of the gas flow port can be appropriately set according to the relationship between the adhesive force and the cohesive force based on the degree of hydrophilicity and hydrophobicity, which acts between the liquid and the base and the cover, and other physical properties such as the viscosity of the liquid. Although the liquid may hardly leak even when the thickness is 1 mm, it is preferably 500 μm or less, and more preferably 100 μm or less. When the gap of the gas flow port is 5 μm, normally air can flow but liquid cannot flow and the gap is
Almost no liquid flows even when it reaches 10 to 15 μm. As the shape of the gas flow port, a slit, other groove, or a micropore structure can be adopted.

Next, the structure of the slide for an optical microscope of the present invention will be described with reference to the drawings. FIG. 1 is a plan view showing one embodiment of the slide for an optical microscope of the present invention, which is an example in which three cells are installed and one pillar is provided near the intersection of the diagonal lines of each cell to prevent bending. . 2 is a sectional view taken along the line AA in FIG. 1, and FIG. 3 is a sectional view taken along the line BB in FIG. The width of the cover 2 is shorter than the front and rear width of the base 1 (see FIG. 3), and the cell 5 (see FIG. 2) is provided between the base 1 and the cover 2.
(Reference) is formed, and a sample is injected here. The left and right sides of the cell 5 are closed by partition walls 3. In the cell 5 shown by the strip-shaped portion between the partition walls, the distance between the base 1 and the cover 2 is kept constant, and the transmitted light in this portion is observed with a microscope. The thickness of this portion, that is, the thickness of the liquid phase filled in the cell 5 is, when observed at a high magnification of several hundred times or more,
Considering the depth of focus and the attenuation of transmitted light, 10 to 20 μm is appropriate, but when observing blood, urine, cells, etc. at 200 to 400 times, which is often used in medical institutions in recent years, it is 40 to 40 μm. 50
μm is suitable. When a sample having a large surface tension is injected, the cover between the partition walls may be bent in the conventional optical microscope slide, which may hinder the microscopic examination. However, in the optical microscope slide of the present invention, this bending is suppressed by the column 15. The uniform sediment component can be microscopically examined. The size of the observation portion (cell) is appropriately selected depending on the purpose of use, but is preferably within the range of 10 mm × 10 mm to 18 mm × 18 mm. In addition, the thickness of the base and cover is closely related to the performance of the condenser and objective lens of the microscope.
The thickness of the base is preferably 0.5 to 1.5 mm, and the thickness of the cover is preferably 0.06 to 0.25 mm. These are selected according to usage conditions, but the thickness of the cover most often used is 0.15 to 0.18 mm according to JIS NO.1-5.

A groove serving as a liquid reservoir 10 is provided on the base 1 along the front edge of the cover 2. Sample is a reservoir 1
When injected to 0, the sample is instantaneously filled in the cell 5 through the slit 7 between the front part of the base and the cover due to the capillary phenomenon, and the surplus is collected in the liquid reservoir 10 in the front part of the cell. When the sample is stored in the liquid reservoir 10, even if the sample is slightly evaporated, it is replenished from the liquid reservoir 10 to the cell 5 by a capillary phenomenon.
No bubbles are generated inside the cell. Therefore,
By using the optical microscope slide of the present invention, a sample for microscopic examination can be prepared very easily. 9
Has a narrow slit shape and has the function of the minute gas flow port described above, and when the sample is injected, the internal air is released from here, but even if the sample fills the cell, or Even if the optical microscope slide is tilted, the sample hardly leaks to the outside. According to the test results, in the case of the water-based sample, the appropriate interval is 10 to 20 μm.

By the way, since the thickness of the liquid phase filled in the cell is thin and the sample is protected by a strong force in this region by the capillary phenomenon, in the case of a sample having a large surface tension, the cover is curved toward the base side. I have something to do. Also, when a sample less than the capacity of the cell is put, the cover may be curved toward the base due to surface tension. When observing at high magnification, generally the sample (liquid phase) is made thin, and the capillary force (the force of adhesion of the liquid to the base and cover) holds the sample even stronger, and the distance between the base and the cover increases. A force that narrows the work. Due to these phenomena,
The distribution of the formed components of the sample may not be uniform depending on the field of view, resulting in a false diagnosis. In the optical microscope slide of the present invention, since the column is provided inside the cell, the cover does not bend due to the force acting on the sample, surface tension, or the like.

For precise observation at high magnification, a thin cover is required due to the design of the optical system of the microscope. The specific thickness of the cover varies slightly depending on the microscope and manufacturer, but is often specified as 0.17 mm at 1000 times magnification. With this thickness, even a glass cover bends to the base side to some extent, and the liquid phase becomes thin in the central portion of the cell, but it is even more so in the case of a plastic cover. According to the slide of the present invention, it is possible to prevent the cover from bending even in such a case.

When a microscope is used, the light transmitted through the sample inside the cell and the base and cover is magnified and observed by the optical system of the microscope. Therefore, the base and the cover are required to have high optical isotropy and flatness. However, due to the progress of mold manufacturing technology and plastic molding technology at present, molded products comparable to glass have been obtained.

The optical microscope slide shown in FIG. 1 is integrally formed by laminating two kinds of resin plates. However, as apparent from the sectional views shown in FIGS. In view of the structure of the reservoir and the structure of the slit between the base and the cover, so-called integral molding cannot be performed. Therefore, after the base portion and the cover portion are separately molded, for example, the partition wall 3 and the cover 2 or the partition wall 3 and the base 1 are fusion-bonded by ultrasonic waves or the like, or are bonded by a plastic adhesive. It is necessary to make one by such methods. In the present invention, “made integrally” means such a structure. When actually making a slide for an optical microscope, the cover is cut into a strip of a large sheet, and the other parts (base, support pillars and partition walls) are integrally molded with a mold, and the cover and partition walls are fused together. Wearing or adhering is most efficient. There is also a method of inserting the pillar between the base and the cover and then fusing or adhering the two, but considering the difficulty of positioning the pillar or the pillar moving and shifting in the cell after completion. By integrally molding the same material, including the columns, when manufacturing the base portion, the problems of positioning and misalignment can be solved. The column and the cover do not necessarily need to be fused or bonded.

FIG. 4 is also a plan view showing one embodiment of the slide for an optical microscope of the present invention, and FIGS. 5, 6 and 7 are sectional views taken along lines CC, DD and EE in FIG. 4, respectively. Is shown. The structures of the base 1, cover 2, partition wall 3 and cell 5 shown in these figures are the same as those described with reference to FIGS. 1 to 3 above, and the relationship between the thickness of the cell and the observation magnification of the microscope,
The description regarding the area of the observation portion of the cell, the thickness of the base and the cover, the function of the sample being instantaneously filled in the cell by the capillary phenomenon, the action of the slits 7 and 9 and the method for producing a slide for an optical microscope are also shown in FIGS. This is the same as the description regarding FIG.

However, in the slide for an optical microscope shown in FIGS. 4 to 7, a tapered portion 8 is provided between the cell 5 and the grooves 4 and 6 so that the distance between the base 1 and the cover 2 gradually increases toward the outside. ing. Here, the shape of the tapered portion may be, for example, a trumpet shape or any other shape, as long as the liquid can be sucked into the center of the cell by the capillary phenomenon. Further, slits 7 and 9 are provided between the front and rear edges of the base and the front and rear edges of the cover, respectively, so that gas flows but liquid does not. Further, a sample injection port 11 communicating with the groove 4 is provided. Furthermore, even if the sample leaks to the outside for some reason and adheres between the slits 7 and 9 and the weir 13,
Can't spill out because of. Therefore, if a person who handles the sample holds the outside of the weir 13, the sample does not come into direct contact with the body, and it is possible to prevent the infection of harmful virus such as hepatitis by the inspection of the medical institution. This is one of the advantages of the present invention. Further, when the sample injection amount is large and the sample is also accumulated in the grooves 4 and 6, if only the sample injection port 11 is closed, only the slits 7 and 9 communicate with the outside air. Since the slits are narrow, the air flows. However, the liquid does not leak out.

The optical microscope slide of the present invention may be provided with a convex scale line on the cell-side surface of the base. The convex cross section of the scale line and the shape of the ridge may be any shape. In order that the image of the scale line can be observed sharply, it is most preferable that the cross section of the top has an acute angle and a constant height, that is, the shape of a triangular pyramid. The scale line may be provided on only a part of the cell or on the entire surface.
Further, the graduation lines are preferably arranged in a grid pattern (see FIG. 8) or parallel lines (see FIG. 9), but may have other shapes. In addition, when there is a possibility that the cell membrane of the sample is damaged by the convex portion having a sharp cross section, it is preferable to crimp the top portion.

Furthermore, it is necessary to consider that the depth of focus of the microscope is very shallow. Specifically, about 300 times about 5 ~
8μm, 700 times about 1.5-2.5μm, 1000 times about 0.9-1μ
m or lower. In other words, the graduation line is observed as a sharp straight line only when the focal point is aligned with the top of the graduation line, since only a very small area can be seen.

Even if the scale line has the shape of a triangular pyramid as described above, two parallel lines are formed when the focus is on the middle side, and a scale line is formed when the focus is on the top. I can't observe. It is considered that the height of the graduation line is up to about one third of the cell thickness under normal use conditions, so that the graduation line cannot be observed in more than half of the area. Therefore, as shown in FIG. 10, if a conical projection is provided on a part of the scale line and its height is matched with the distance between the base and the cover, the scale can be observed regardless of which part of the cell is focused. . For example, when focusing near the base surface, the field of view of the microscope is shown in FIG.
It is observed as shown in FIG. 11A, and when it is focused near the cover, it is observed as shown in FIG. 11B. Therefore, no matter which part of the sample is observed by moving the focus up and down, the sample and the clear scale can be observed at the same time. Some conventional optical slides integrally molded with an optical microscope have a scale on the outside of the cell, but the scale cannot be observed at all in the microscope's field of view. This is obvious considering the relationship between the depth of focus and the position of the scale. In the present invention, since the scale line is provided on the cell-side surface of the base, not only can it be clearly observed with the sample, but the scale line can be shaped as shown in FIG. The scale and sample can be observed simultaneously.

It is not practical to provide a ridge-like protruding portion on the inner surface of the cell because it is very difficult and expensive to use glass. Further, it is relatively easy to engrave a groove-shaped scale line on the glass, but the scale cannot be effectively used due to the depth of focus, as described above. But,
Once the plastic is processed in that way,
It is easy to duplicate a large number of molded products. Therefore, it can be said that the slide for an optical microscope provided with the graduation lines having a complicated shape as shown in FIG. 10 is a structure that makes the best use of the characteristics of plastic.

The optical microscope slide of the present invention may be provided with one cell on one base, and as shown in FIGS. 1, 4, 8, 9 and 12, two or more cells may be provided. Can be provided. Recently, many microscopic examinations have been carried out in medical institutions and the like, so that providing two or more cells on one base can greatly improve the efficiency of the examination.

Furthermore, colored plastics can be used in the present invention. Depending on the nature of the sample and the purpose of observation, it is often necessary to observe through a filter. For example, the green filter is often used in observing cells. The use of pre-stained optical microscope slides eliminates the need for filters.
It is possible to tint the base glass to give it the same function as the filter, but it is very expensive and rarely is the tinted base glass used. However, coloring a plastic is extremely easy and inexpensive.

FIGS. 13 and 14 use a base having a convex shape and a triangular cross section, a line interval of 0.3 mm, a width of 19 μm and a height of 11 μm and having orthogonal graduation lines, and blood is diluted with physiological saline. FIG. 8 is a view of red blood cells 17 taken with a microscope and enlarged 2 times to 200 times and 320 times. From this, it is recognized that both red blood cells and scale lines can be clearly observed, and counting of red blood cells and the like becomes easy. Further, since the scale line has a height of 11 μm, the scale line can be clearly observed even when the focus is moved and observed.

[0024]

EXAMPLES The present invention will be specifically described below with reference to examples. The present invention is not limited to these examples.

Example 1 A part (26 mm × 76 mm) in which a base, a support and a partition were integrally molded was made by molding an acrylic resin, and this and a polycarbonate plate (18 mm × 66 mm) were bonded together with an adhesive, An optical microscope slide having a hemispherical column with a diameter of 0.5 mm was prepared at the intersection of the diagonal lines of the cell. A plan view and a cross-sectional view of the produced optical microscope slide are shown in FIGS.

Example 2 In the same manner as in Example 1, a part (26 mm × 76 mm) in which an acrylic resin was molded to integrally form a base, a pillar and a partition was prepared, and this and a polycarbonate plate (18 mm × 66 mm) were manufactured. An optical microscope slide having a hemispherical column with a diameter of 0.5 mm was prepared at the intersection of diagonal lines of the cells by bonding with an adhesive.
Plan views and cross-sectional views of the manufactured optical microscope slides are shown in FIGS. The difference from Example 1 is that a taper portion is provided at the sample injection port and the gas flow port to facilitate suction of the sample to the center of the cell.

Examples 3 and 4 In the same manner as in Example 1, a part (26 mm x 76 mm) in which an acrylic resin was molded to integrally form a base, a support and a partition wall was prepared, and this was combined with a polycarbonate plate (18 mm x 66 mm). And were adhered with an adhesive to prepare a slide for an optical microscope.
A plan view of the manufactured optical microscope slide is shown in FIG. 8 (Example 3) and FIG. 9 (Example 4), and a front view and a side view of scale lines are shown in the upper and lower views of FIG. 10, respectively. The difference from Example 1 is that a grid (Example 3) or parallel line (Example 4) scales are formed on the base during molding of the base.

Example 5 Two recesses were formed per cell for forming columns in a molding die.
The same operation as in Example 1 was carried out except that the number was provided, to obtain a slide for an optical microscope having two columns per cell. The plan view and cross-sectional view are shown in FIGS.

Example 6 Three recesses were formed per cell for forming columns in a molding die.
The same operation as in Example 1 was carried out except that the slides were provided, so that a slide for an optical microscope having three columns per cell was obtained. The plan view and sectional view are shown in FIGS.

Test Example 1 The same as Example 1 except that a slide for an optical microscope (hereinafter, abbreviated as slide A) in which one column was provided in the center of the cell prepared in Example 1 and no column was provided. Shaped optical microscope slides (hereinafter, abbreviated as slide B) are prepared, and a blood sample having a high surface tension is injected into each slide, and the points a to c in the cell shown in FIG. 21 are centered. The number of red blood cells in the formed components in the visual field was measured, and the results are shown in Table 1. In Table 1, each field of view (points a to c)
The average value of the three measurements is shown.

[Table 1] As shown in Table 1, in slide A, the cover was not curved, and the red blood cell count of the formed particles in the measured sample was almost constant no matter which visual field inside the cell was measured. However, in slide B, the cover was curved due to the surface tension of the sample, and the measured number of red blood cells in the sample varied considerably depending on the visual field inside the cell. Although the pillar of the slide A exists at the same center as the point b, the pillar can be clearly recognized as a pillar during the microscopic examination, and it cannot be mistaken for a formed component (red blood cells, white blood cells, etc.) in the sample. There wasn't.

[0031]

According to the present invention, there is provided a slide for an optical microscope in which the thickness of the liquid phase of the sample is kept constant irrespective of the type and amount of the sample and the sample does not adhere to the human body during handling. Provided.

[Brief description of drawings]

FIG. 1 is a plan view showing an optical microscope slide obtained in Example 1. FIG.

FIG. 2 is A in the optical microscope slide shown in FIG.
It is a sectional view of -A.

FIG. 3 is B in the optical microscope slide shown in FIG.
It is a sectional view of -B.

FIG. 4 is a plan view showing an optical microscope slide obtained in Example 2.

FIG. 5 is C in the optical microscope slide shown in FIG.
It is sectional drawing of -C.

FIG. 6 is D in the optical microscope slide shown in FIG.
It is sectional drawing of -D.

FIG. 7 E in the optical microscope slide shown in FIG.
It is a sectional view of -E.

FIG. 8 is a plan view of a slide for an optical microscope in which a grid-like scale line is provided on the cell-side surface of the base obtained in Example 3.

FIG. 9 is a plan view of a slide for an optical microscope in which a scale line parallel to the cell side on the base obtained in Example 4 is provided.

FIG. 10 is a front view (top) and a plan view (bottom) of a part of one graduation line.

11 (a) is a field of view obtained by observing with a microscope a portion near the slide surface of the cell (focusing on the ridge of the convex portion of the scale line) when the scale line in FIG. 8 has the shape as in FIG. And (b) shows an image of the visual field in the vicinity of the cover.

FIG. 12 is a plan view of a slide for an optical microscope in which a large number of cells are provided on one base.

FIG. 13 is a diagram of erythrocytes and graduation lines that are magnified 200 times by photographing a erythrocyte diluted with a physiological saline solution with a microscope and using a base having a graduation line having a triangular cross-section. Is shown.

FIG. 14 is a diagram of red blood cells and a scale line which are 300 times magnified by taking a red blood cell diluted with a physiological saline solution with a microscope and using a base having a convex scale line having a triangular section. Is shown.

FIG. 15 is a plan view showing an optical microscope slide obtained in Example 5.

16 is a cross-sectional view of F-F in the optical microscope slide shown in FIG.

17 is a cross-sectional view of GG in the optical microscope slide shown in FIG.

FIG. 18 is a plan view showing an optical microscope slide obtained in Example 6.

19 is a cross-sectional view taken along line HH of the optical microscope slide shown in FIG.

20 is a cross-sectional view taken along line I-I of the optical microscope slide shown in FIG.

21 is a diagram showing microscopic inspection points inside a cell in Test Example 1. FIG.

[Explanation of symbols]

 1 Base 2 Cover 3 Partition 4, 6 Groove 5 Cell 7, 9 Slit Between Base and Cover 8 Tapered Part Between Base and Cover 10 Liquid Reservoir 11 Sample Inlet 12 Scale Line 13 Weir 14 Sample 15 Strut 16 Red Blood Cell

Claims (4)

[Claims] 1. A cell for holding a sample is formed by a base, a cover, a support and a partition, and the base and the cover are made of transparent plastic, and the partition seals the left and right of the cell and, together with the support, the base and the cover. An optical microscope having a function of holding a constant interval, an opening for sample injection in the front part of the cell, and a minute gas flow port through which a gas flows but a liquid does not flow in the rear part of the cell. Slides. 2. A base, a cover, a support, and a partition are integrally made of plastic to form a cell for holding a sample. The length of the cover in the front-rear direction is shorter than that of the base, and the left and right of the cell are the base. The space between the cover and the cover is closed, and grooves are formed on the base along the edges of the front and rear parts of the cover.The gap between the front part of the base and the cover serves as the sample injection port, and the gap between the rear part of the base and the cover. Is a gas flow port, and has a sample holding cell having a predetermined interval partitioned by the left and right partition walls and the front and rear grooves, and a slide for an optical microscope. 3. A base, a cover, a support, and a partition are integrally made of plastic to form a cell for holding a sample. The length of the cover in the front-rear direction is shorter than that of the base, and the left and right of the cell are the base. The space between the cover and the cover is closed by a partition wall, and the front and rear parts of the base are provided with a tapered portion in which the distance between the base and the cover gradually increases toward the outside, and the front and rear parts of the cover are provided outside the tapered portion. A groove is provided on the base along the edge of the base, a gap between the base and the tip of the cover serves as a gas flow port, and a cell for holding a sample having a predetermined space defined by the left and right partition walls and front and rear tapered portions. The slide for an optical microscope is characterized in that there is a sample injection port communicating with the groove in the front part, and the edges of the front part and the rear part of the base are weirs. 4. The slide for an optical microscope according to claim 1, wherein a convex scale line is provided on the cell-side surface of the base.