CN218726545U - Slide glass for quantitative microscopic examination of body fluid sample - Google Patents
Slide glass for quantitative microscopic examination of body fluid sample Download PDFInfo
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- CN218726545U CN218726545U CN202122394273.7U CN202122394273U CN218726545U CN 218726545 U CN218726545 U CN 218726545U CN 202122394273 U CN202122394273 U CN 202122394273U CN 218726545 U CN218726545 U CN 218726545U
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Abstract
The utility model discloses a glass slide for quantitative microscopic examination of a body fluid sample, which comprises a glass slide body; the microscopic examination frame is arranged on the upper surface of the glass slide body, is of an open frame structure, is surrounded into a sample adding microscopic examination area for dripping the body fluid sample to complete microscopic examination determination, and is provided with at least one broken opening for discharging redundant body fluid sample. The utility model discloses the advantage lies in ensuring that the body fluid sample in the application of sample mirror examination district is the even thin one deck of thickness, realizes the ration count, and the definition is better when making artifical microscope mirror examine, shoot, avoids appearing lou examining the phenomenon, ensures to carry out body fluid sample detection by the tangible composition analytical instrument of perfect adaptation, improves the mirror examination analytical accuracy, is favorable to promoting medical technology's progress, is favorable to different hospital's results to mutually recognize, avoids causing unnecessary economic burden for the patient.
Description
Technical Field
The utility model belongs to the technical field of the medical treatment consumptive material technique and specifically relates to a body fluid sample ration slide glass for microscopic examination is related to.
Background
At present, a glass slide commonly used for manual microscope microscopy in clinic is a rectangular glass sheet, a cover glass is covered after a sample is coated in a microscopy area, high power microscopy is carried out under a microscope, and the number range of visible components in each high power microscopy field is obtained as a detection result by counting the visible components in a plurality of fields. For the manual microscopic analysis of the visible components in body fluid samples (such as urine, feces, cerebrospinal fluid, serosal cavity hydrops, vaginal secretion and the like), the direct smear method is needed for preparing the slices, and the counting result has larger difference due to different sample adding amount, sample consistency, cover glass size and the like.
Along with the automatic and intelligent development of the visible component analyzer, the visible component analyzer with two different film production modes appears on the market: one is an analyzer (such as a stool visible component analyzer) using a planar laminar flow technique, which counts samples by microscopic photographing and image recognition while a sample liquid flows through a detection cell; because the sample composition is complicated, consequently for preventing the liquid path jam, the measuring cell has certain thickness, and the tangible composition can be three-dimensional when passing through the measuring cell and distribute, and the tangible composition definition that is not on the focusing surface at this moment is relatively poor or can't see, easily causes the missed detection phenomenon, leads to the counting result inaccurate. The other is an analyzer adopting a special slide set, such as a slide set in which a cover glass and a slide glass are adhered together to finish slide making by capillary action, or a slide set in which the cover glass and the slide glass are automatically assembled by an instrument to finish slide making; because the sample is complicated, the height of a detection pool of the slide kit for completing slide production by capillary action has great influence on slide production and detection results, the thickness of sample liquid is higher due to the overhigh height of the detection pool, the detection of visible components which are not on the focal plane is avoided, and the hole is blocked due to the overlow height of the detection pool, so that the problems of detection leakage, hole blockage and the like exist in the slide kit, and the slide kit is not favorable for accurate counting or higher failure rate; and the slide kit for preparing the slide by automatic assembly of the instrument has the advantages that the thickness of the sample liquid under the cover glass is related to the viscosity of the sample, and the thickness of the sample liquid is variable after automatic cover glass is performed, so that quantitative analysis cannot be performed. In summary, various problems existing in the existing body fluid sample visible component analyzer are not beneficial to the examiner to jointly research and solve clinical problems, the progress of medical technology is not promoted, the results of different hospitals are not mutually acknowledged, and unnecessary economic burden is caused to the patient.
Disclosure of Invention
The utility model aims at providing a body fluid sample ration slide glass for microscopic examination.
In order to achieve the above purpose, the utility model can adopt the following technical proposal:
body fluid sample ration slide glass for microscopic examination, its characterized in that: comprises that
A slide body; and
the microscopic examination frame is arranged on the glass slide body, is of an open frame structure, is surrounded into a sample adding microscopic examination area for dripping the body fluid sample to complete microscopic examination judgment in the middle, and is provided with at least one broken opening for discharging redundant body fluid sample.
Furthermore, an anti-overflow frame is arranged on the glass slide body, the anti-overflow frame is of a closed frame structure, an anti-overflow area for preventing the body fluid sample from overflowing is formed by surrounding the middle of the anti-overflow frame, and the microscopic examination frame is located in the anti-overflow area.
Further, the microscopic examination frame is positioned in the center of the overflow-proof area.
Furthermore, the microscopic examination frame and the spill-proof frame are both formed by paint coatings coated on the glass slide body, and the coating thickness of the paint coatings is 10-50 micrometers; certainly, the microscopic examination frame and the overflow prevention frame can also be formed by adhesive tapes attached to the glass slide body, and the thickness of the adhesive tapes is 10-50 μm; of course, the microscopic examination frame and the overflow prevention frame can also be formed by grooves formed in the glass slide body.
Furthermore, the anti-overflow frame is of a rectangular structure, the microscopic examination frame is of a rectangular structure consistent with the center of the anti-overflow frame, four break openings are formed in the microscopic examination frame, and the four break openings are respectively located at four corners of the microscopic examination frame; certainly, the anti-overflow frame can also be of a circular structure, the microscopic examination frame is of a circular structure consistent with the center of the anti-overflow frame, and four break openings are uniformly arranged on the microscopic examination frame at intervals along the circumferential direction; in addition, the anti-overflow frame can also be of a polygonal structure, the microscopic examination frame is of a polygonal structure with the same shape and the same center as the anti-overflow frame, and each included angle of the microscopic examination frame is provided with one broken opening.
The utility model has the advantages that the microscopic examination frame with an open frame structure is arranged on the glass carrier body, thereby when the body fluid sample is dripped into the microscopic examination area at the inner side of the microscopic examination frame, the extrusion of the cover glass ensures that the redundant body fluid sample flows out from the disconnection port on the microscopic examination frame, the body fluid sample in the microscopic examination area is ensured to be a thin layer with uniform thickness, the quantitative counting is realized, the microscopic examination of an artificial microscope is ensured, the definition is better when photographing, the omission phenomenon is avoided, the perfect adaptation of a visible component analyzer is ensured to detect the body fluid sample, the accuracy of microscopic examination analysis is improved, the progress of medical technology is facilitated, the mutual recognition of different hospital results is facilitated, and the unnecessary economic burden of a patient is avoided; in addition, still enclose the anti-overflow frame that is equipped with a closed frame type structure in the frame outside is examined to the mirror, can prevent effectively that the body fluid sample that is extruded by the cover glass from flowing outward to the laboratory bench at will, guarantee clean, clean and tidy of experiment operation.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a bottom view of fig. 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
As shown in fig. 1 and 2, the glass slide for quantitative microscopic examination of body fluid samples of the present invention comprises a glass slide body 1, a microscopic examination frame 2 is arranged on the glass slide body, the microscopic examination frame 2 is an open frame structure, the middle part of the glass slide body is enclosed into a sample adding microscopic examination region 3 for dropping body fluid samples to complete microscopic examination determination, and the microscopic examination frame 2 is provided with at least one disconnection port 4 for discharging redundant body fluid samples; in addition, still be provided with an anti-overflow frame 5 on the upper surface of slide glass body 1, anti-overflow frame 5 is closed frame type structure, its middle part encloses into the anti-overflow district 6 that is used for preventing the body fluid sample from spilling over, at this moment, frame 2 should be located anti-overflow district 6 to the mirror, specifically can be located the center department of anti-overflow district 6, disconnected opening 4 on the frame 2 is examined to the mirror just can communicate application of sample mirror examination district 3 and anti-overflow district 6, make 3 discharged unnecessary body fluid samples from application of sample mirror examination district flow to anti-overflow district 6 in, effectively prevent that the body fluid sample from flowing to the laboratory bench at will, avoid polluting the laboratory bench, guarantee that experiment operation's is clean, clean and tidy.
Specifically, frame 2 and anti-overflow frame 5 are examined by the mirror and are constituted by coating the paint coating on slide glass body 1, and anti-overflow frame 5 and the mirror examine the paint coating thickness of frame 2 unanimously, be 10 mu m ~50 mu m, ensure that the coverslip steadily covers on anti-overflow frame 5, and simultaneously, the coverslip can also extrude the sample liquid (its highly should be higher than the thickness of paint coating when the dropwise add of this sample liquid) of dropwise add in sample mirror examination district 3, make unnecessary sample liquid extrude outside mirror examination frame 2 through break-off opening 4, ensure that mirror examination frame 2 inboard can leave even one deck sample liquid, make the definition of follow-up mirror examination shooing good, ensure can perfect adaptation tangible composition analytical instrument carry out body fluid sample detection, improve the accuracy of mirror examination analysis, realize the ration count. Certainly, the microscopic examination frame 2 and the spill-proof frame 5 can also be formed by adhesive tapes attached to the glass slide body 1, and the thickness of the adhesive tapes is ensured to be 10-50 μm; in addition, the microscopic examination frame 2 and the overflow preventing frame 5 can also be formed by grooves arranged on the glass slide body 1.
Further, because the conventional cover glass is generally in a rectangular structure, the overflow-preventing frame 5 is also in a rectangular structure and can be consistent with the cover glass in size or slightly smaller than the cover glass, at this time, the microscopic examination frame 2 can be in a rectangular structure consistent with the center of the overflow-preventing frame 5, the microscopic examination frame 2 is also consistent with the overflow-preventing frame 5 in shape, and the four corners of the microscopic examination frame 2 in the rectangular structure are provided with the breaking openings 4, namely the four breaking openings 4 are respectively located at the four corners of the microscopic examination frame 2. Certainly, the anti-overflow frame 5 may also be in a circular structure or a polygonal structure, when the anti-overflow frame 5 is in a circular structure, the corresponding microscopic examination frame 2 is also in a circular structure consistent with the center of the anti-overflow frame 5, and at this time, four cut-off openings 4 may be uniformly formed on the microscopic examination frame 2 at intervals along the circumferential direction; when the anti-overflow frame 5 is in a polygonal structure, the corresponding microscopic examination frame 2 is also in a polygonal structure with the same shape and the same center as the anti-overflow frame 5, and at the moment, a broken opening 4 is formed at each corner of the microscopic examination frame 2.
The specific examples of the slide glass for quantitative microscopic examination of the body fluid sample are as follows:
example one, vaginal secretion has a composition analysis:
uniformly mixing the vaginal secretion sample, dripping one drop of the mixture to the center of a microscopic frame with the thickness of 50 mu m of the paint coating, covering a cover glass, and placing the mixture under a microscope to observe 5 visual fields, wherein the number of red blood cells in each visual field is respectively 6, 10, 12, 8 and 13, and the red blood cell content calculation method comprises the following steps: the 22-field eyepiece high power lens field diameter =22/40=0.55mm, and the field area is: 3.14X (0.55/2) 2 =0.237mm 2 The volume of the sample liquid in the visual field was 0.237 × 0.05=0.01185 μ L. Red blood cell concentration per field = red cell number per field ÷ sample volume per field, the results are given in the following table:
the red blood cell concentration of the vaginal secretion samples was averaged based on the number of fields of view required for microscopic examination in each laboratory. For example: the mean red blood cell concentration of the above 5 fields was 827/. Mu.L.
Example two, urine formation analysis:
10mL of urine is taken, centrifuged at 4000rpm for 10min, 9.8mL of supernatant is discarded by a micropipette, the residual sediments are sucked and uniformly stirred, a drop is dripped to the center of a microscopic frame with the paint coating thickness of 50 mu m, a cover glass is covered, 5 visual fields are observed under a microscope, the number of white blood cells in each visual field is respectively 3, 7, 5, 6 and 2, and the calculation method of the content of the white blood cells comprises the following steps: the 22-field eyepiece high power lens field diameter =22/40=0.55mm, and the field area is: 3.14X (0.55/2) 2 =0.237mm 2 The volume of the sample liquid in the visual field is 0.237 × 0.05=0.01185 μ L; sample centrifugation concentration factor = volume before centrifugation/sediment volume after centrifugation =10mL/0.2ml =50 mL, white blood cell concentration per field = number of white blood cells per field ÷ sample volume per field ÷ concentration factor, the results are shown in the following table:
the leukocyte concentration of the urine sample is calculated according to the number of visual fields required to be examined under the microscope in each laboratory. For example: the mean of the leukocyte concentrations in the 5 fields above is 8/μ L.
Can clearly learn by above-mentioned example one and example two, adopt body fluid sample ration microscopic examination use slide glass, can ensure that the body fluid sample in the microscopic examination district of application of sample is the even thin one deck of thickness, realize the ration count, the definition is better when making artifical microscope microscopic examination shoot, avoid appearing the phenomenon of lou examining, ensure can the perfect adaptation tangible composition analysis instrument carry out body fluid sample test, improve the accuracy of microscopic examination analysis, be favorable to promoting the progress of medical technology, be favorable to different hospitals to mutually recognize the result, avoid causing unnecessary economic burden for the patient.
Claims (9)
1. A slide glass for quantitative microscopic examination of a body fluid sample is characterized in that: comprises that
A slide body; and
the microscopic examination frame is arranged on the glass slide body, is of an open frame structure, is surrounded into a sample adding microscopic examination area for dripping the body fluid sample to complete microscopic examination judgment in the middle, and is provided with at least one broken opening for discharging redundant body fluid sample.
2. The slide glass for quantitative microscopic examination of a body fluid sample according to claim 1, characterized in that: the anti-overflow frame is arranged on the glass slide body and is of a closed frame structure, an anti-overflow area used for preventing the body fluid sample from overflowing is formed by surrounding the middle of the anti-overflow frame, and the microscopic examination frame is located in the anti-overflow area.
3. The slide glass for quantitative microscopy of a body fluid sample according to claim 2, wherein: the microscopic examination frame is positioned in the center of the anti-overflow area.
4. The slide glass for quantitative microscopic examination of a body fluid sample according to claim 2, characterized in that: the microscopic examination frame and the anti-overflow frame are both formed by paint coatings coated on the glass slide body, and the coating thickness of the paint coatings is 10-50 microns.
5. The slide glass for quantitative microscopic examination of a body fluid sample according to claim 2, characterized in that: the microscopic examination frame and the anti-overflow frame are both formed by adhesive tapes attached to the glass slide body, and the thickness of each adhesive tape is 10-50 microns.
6. The slide glass for quantitative microscopic examination of a body fluid sample according to claim 2, characterized in that: the microscopic examination frame and the anti-overflow frame are both formed by grooves formed in the glass slide body.
7. The slide glass for quantitative microscopy of a body fluid sample according to claim 2, wherein: the anti-overflow frame is of a rectangular structure, the mirror inspection frame is of a rectangular structure consistent with the center of the anti-overflow frame, four break openings are formed in the mirror inspection frame, and the four break openings are located at four corners of the mirror inspection frame respectively.
8. The slide glass for quantitative microscopy of a body fluid sample according to claim 2, wherein: the anti-overflow frame is of a circular structure, the microscopic examination frame is of a circular structure consistent with the center of the anti-overflow frame, and four cut-off openings are uniformly formed in the microscopic examination frame in a circumferential direction at intervals.
9. The slide glass for quantitative microscopic examination of a body fluid sample according to claim 2, characterized in that: the anti-overflow frame is of a polygonal structure, the mirror inspection frame is of a polygonal structure with the same shape and the same center as the anti-overflow frame, and each included angle of the mirror inspection frame is provided with one cut-off opening.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122394273.7U CN218726545U (en) | 2021-09-30 | 2021-09-30 | Slide glass for quantitative microscopic examination of body fluid sample |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122394273.7U CN218726545U (en) | 2021-09-30 | 2021-09-30 | Slide glass for quantitative microscopic examination of body fluid sample |
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| CN218726545U true CN218726545U (en) | 2023-03-24 |
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