TW202403281A - Biochip detection device - Google Patents

Abstract

A biological chip detection device includes a base and a reaction module. The reaction module is arranged on the base. The reaction module includes a reaction membrane, a diffusion membrane, a filter membrane and an upper cover stacked from bottom to top. The reaction membrane is adjacent to the base. A liquid suction area around the hole, the upper cover is arranged on the base and includes a first operation area and a second operation area adjacent to the first operation area, and the first operation area of the upper cover has a plurality of droplets for dropping the liquid to be tested. An inlet hole, the second operation area has a reaction opening hole for dripping the colorant. The filter membrane is arranged at a position corresponding to the first operation area of the upper cover, and the reaction membrane and the diffusion membrane can move between the first operation area and the second operation area corresponding to the upper cover.

Description

Biochip detection device

The present invention relates to a biological chip detection device, and in particular to a technical field that can simultaneously detect a variety of different detection specimens and improve the accuracy of detection results.

Immunochromatography technology has played a very important role in today's medical system. It can be used to qualitatively or quantitatively measure changes in various biological indicators to provide laboratory personnel or medical personnel with information for disease diagnosis or drug treatment.

The vast majority of test strips currently on the market are qualitatively designed to allow the examiner to obtain the test results through visual inspection. Although a variety of different test items can be tested at the same time, the coloring agent needs to flow to the reactions of multiple liquids to be tested. zone, the colorant flows sideways in a single direction, and then flows to each reaction zone sequentially to react with the liquid to be tested. This method will cause the capacity of the colorant to flow to each reaction zone to gradually change. Rarely, due to the uneven distribution of the coloring agent, the liquid to be tested receives different coloring agents, resulting in inspection errors, or even inaccuracies in inspection. Therefore, how to use innovative detection device designs to simultaneously detect a variety of different detection items and improve detection accuracy is an urgent problem to be solved.

In addition, since the test specimen is taken from the human body or animal, in addition to the liquid part, sometimes the test specimen also contains some tissue debris, which may affect the test results.

In view of this, the object of the present invention is to provide a method that can detect multiple different test specimens at the same time. Only a trace amount of the test liquid can be reacted with the coloring agent, which can not only reduce the difficulty of inspection and greatly increase the detection time. The sample also utilizes the peripheral diffusion flow of the chromogen to evenly combine and react with all the liquids to be tested at the same time, which can effectively improve the accuracy of the test results.

An embodiment of the biochip detection device of the present invention includes a base and a reaction module. The reaction module is set on a base. The reaction module includes a reaction membrane, a diffusion membrane, a filter membrane and an upper cover stacked from bottom to top. The reaction membrane is adjacent to the base. The diffusion membrane has at least one liquid suction opening and is located in the liquid suction opening. There is a liquid suction area around the hole. The upper cover is arranged on the base and includes a first operating area and a second operating area adjacent to the first operating area. The first operating area of the upper cover has a plurality of openings for dripping a liquid to be tested. The dropping hole, the second operating area has a reaction opening for dripping a coloring agent. The filter membrane is arranged at a position corresponding to the first operating area of the upper cover, and the reaction membrane and the diffusion membrane are movable between the positions corresponding to the first operating area and the second operating area of the upper cover.

In another embodiment, the reaction membrane includes a body and a tie rod connected to the body, and the diffusion membrane is disposed on the body.

In another embodiment, the body size of the reaction membrane is equal to the size of the diffusion membrane, and the reaction membrane contacts the liquid absorption opening and the local liquid absorption area.

In another embodiment, a flange surrounding the edge of the base is provided on one side of the base close to the upper cover. The body of the reaction membrane is disposed in the area surrounded by the flange. A limiting groove is formed on one side of the flange. The pull rod of the reaction membrane Extends through the limiting groove and is exposed on the base and upper cover.

In another embodiment, the pore diameter of the reaction opening is larger than the pore diameter of the liquid-absorbing opening, and when the diffusion membrane moves to a position corresponding to the second operating area, the liquid-absorbing area is partially exposed to the reaction opening.

In another embodiment, the wall of each drip hole is conical.

In another embodiment, the upper cover is provided with a recess, which is disposed in the first operating area, and the filter membrane is disposed and positioned in the recess.

In another embodiment, the drip holes are arranged at intervals outward from the center of the first operating area.

In another embodiment, the reaction module includes two reaction membranes, two diffusion membranes and two filtration membranes. The upper cover includes two first operating areas and two second operating areas. The two first operating areas are in phase. They are arranged adjacent to each other, and each reaction membrane and diffusion membrane respectively move between positions corresponding to a first operating area and a second operating area.

In another embodiment, each drop-in hole is for a liquid to be tested to be dropped into and penetrate through the filter membrane, and the liquid suction hole is opened to the reaction membrane to form an area to be tested, and the reaction membrane moves from the first operating area to the second In the operating area, the reaction opening and the suction opening allow a colorant to be dropped into the center around the area to be measured. The colorant is adsorbed by the liquid suction area and diffuses towards the surrounding area to form a reaction with each other. , and obtain the final test results.

In another embodiment, the coloring agent includes a reactive antibody and colloidal gold, and the capacity of the test solution is between 0.1 microgram and 0.2 microgram.

The biological chip detection device of the present invention can detect multiple detection specimens at the same time. It only requires a trace amount of the liquid to be tested to react with the coloring agent. It can not only reduce the difficulty of inspection and greatly increase the number of detection samples, but also utilize the coloring agent. The peripheral diffusion flow method evenly combines and reacts with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the test results, which can effectively improve the accuracy of the test results as a whole. In addition, the biological chip detection device of the present invention also includes a filter membrane, which can filter out tissue debris or foreign matter in the test subject. Moreover, the reaction membrane of the present invention is movable and can be moved before dripping the coloring agent. First, move the reaction membrane from the first operating area to the second operating area so that the coloring agent can be dripped from the reaction opening to avoid affecting the color development of the final test result.

Please refer to FIG. 1, FIG. 2, and FIG. 3, which is an embodiment of the biochip detection device of the present invention. The biological chip detection device of the present invention is applied to immunochromatography technology. The biological chip detection device of this embodiment includes a base 10 and a reaction module 20 . The reaction module 20 is arranged on the base 10 .

The reaction module 20 includes a reaction membrane 21, a diffusion membrane 22, a filter membrane 24 and an upper cover 23 stacked from bottom to top. The reaction film 21 is adjacent to the base 10 . The reaction membrane 21 can be a nitro-cellulose membrane, a PVDF membrane (polyvinylidone (PVDF) membrane), a nylon membrane, etc., and is used to adsorb the protein to be detected in the test liquid 40 . The diffusion film 22 has at least one liquid suction opening 221 and a liquid suction area 222 located around the liquid suction opening 221. The upper cover 23 is engaged with the base 10 through the engagement post A and the engagement hole B provided in the base 10. , and the base 10 is provided with a flange 11 on the surface close to the upper cover 23, so that when the upper cover 23 is engaged with the base 10, an accommodation space is formed between the upper cover 23 and the base 10, and the reaction film 21 and the diffusion film 22 are both arranged on in the accommodation space within the recess 235 .

The upper cover 23 is rectangular and includes a first operating area 231 and a second operating area 232. The second operating area 232 is adjacent to the first operating area 231. The upper cover 23 forms a plurality of drip holes 233 in the first operating area 231 , and the upper cover 23 forms a reaction opening 234 in the second operating area 232 . The dripping holes 233 are arranged at intervals outward from the center of the first operating area 231, and the reaction openings 234 are square. The size of the reaction opening 234 is larger than the pore diameter of the liquid absorbing opening 221 .

The reaction membrane 21 of this embodiment includes a body 211 and a tie rod 212 connected to the body 211. The diffusion membrane 22 is provided on the body 211. A limiting groove 12 is formed on one side of the flange 11 of the base 10 . The tie rod 212 extends from the body 211 and passes through the limiting groove 12 to be exposed on the base 10 and the upper cover 23 . The user pulls the pull rod 212 to move the reaction film 21 and the diffusion film 22 between the upper cover 23 and the base 10 and between the first operating area 231 and the second operating area 232 . The filter membrane 24 can filter the biological tissue debris in the liquid to be tested 40 to prevent the biological tissue debris from affecting the detection results.

The upper cover 23 is provided with a recess 235 in the first operating area 231 . The filter membrane 24 is disposed and positioned in the recess 235 . The recess 235 corresponds to the plurality of drip holes 233 in the first operating area 231 of the upper cover 23 .

As shown in FIG. 3 , when the reaction membrane 21 moves to the first operating area 231 , the dripping hole 233 and the liquid suction opening 221 are connected. After the liquid to be tested 40 is dripped from the dripping hole 233 , the liquid to be tested 40 can filter out tissue debris through the filter membrane 24 and then enter the reaction membrane 21 through the liquid suction opening 221 . The material of the upper cover 23 is transparent, which helps the detector to directly observe the reaction process and status of the detection. The size of the reaction film 21 is substantially equal to the size of the diffusion film 22. The reaction film 21 is in contact with the liquid suction opening 221 and the liquid suction area 222. As long as the reaction film 21 can cover the range of the liquid suction opening 221, it can ensure the desired dripping. It suffices that the liquid (such as the liquid to be tested, the coloring agent and the cleaning liquid, etc.) can fall into the reaction film 21 . The diffusion membrane 22 of this embodiment has a liquid-absorbing opening 221, and the liquid-absorbing opening 221 is circular.

As shown in FIG. 3 , the hole wall of each dripping hole 233 is conical, and the pore diameter on the side close to the reaction membrane 21 is smaller than the pore diameter on the side far away from the reaction membrane 21 . For example, after the liquid to be tested 40 enters the dripping hole 233 , The inlet hole 233 uses the conical hole wall to collect the liquid to be tested 40 at a specific position to avoid overflow, and wait for the liquid to be tested 40 to gradually diffuse in the reaction membrane 21 . After the liquid to be tested 40 is dripped from the dripping hole 233, the liquid to be tested 40 will penetrate through the filter membrane 24 and then pass through the reaction opening 234 and the liquid suction opening 221 to the reaction membrane 21 to form a region to be measured.

Then, as shown in Figures 4 and 5, the pull rod 212 of the reaction film 21 is pulled to move the reaction film 21 and the diffusion film 22 from the first operating area 231 to the second operating area 232, so that the reaction film 21 is formed on the body 211 of the reaction film 21. The area to be measured corresponds to the reaction opening 234. As shown in FIG. 6 , the sides of the drip hole 233 can each have a different drip mark, such as a numerical number, a pattern, or an English letter. In this embodiment, the number 1 is marked next to the drip hole 233 . ,2,3,4,5,6,7. The drip mark corresponds to a piece of test data of the liquid to be tested 40. The test data includes relevant information such as subject information, specimens, and test items. In this way, the examiner can clearly identify each corresponding test item through the drip mark. Testing data can effectively prevent inspectors from making mistakes. Each dripping hole 233 is for dripping a liquid to be tested 40, which can be body fluid or blood. Here, seven dripping holes 233 are used as an example. Each dripping hole 233 drips the liquid to be tested. The test liquids 40 are all different and belong to different test data, in which the test subject data and the specimen are different, but the test items can be the same or different, and can be appropriately adjusted according to the test requirements.

Then, as shown in FIG. 6 , the coloring agent 50 is dropped into the reaction opening 234 . The coloring agent 50 is dripped into the center position surrounded by multiple areas to be measured through the liquid suction openings 221. The coloring agent 50 is adsorbed by the liquid absorption area 222 and diffuses toward the surroundings to flow to the multiple areas to be measured to form reactions with each other. Among them, the liquid absorbing area 222 is located around the liquid absorbing opening 221, so the coloring agent 50 is subject to the capillary action of the liquid absorbing area 222, and the coloring agent 50 flows uniformly and simultaneously toward multiple areas to be measured at a free angle until the liquid absorbing area. 222, so that all areas to be measured can receive the coloring agent 50 evenly, so that the coloring agent 50 and the liquid to be measured 40 can combine and react. It is worth noting that the capacity of the liquid to be tested 40 is between 0.1 microgram and 0.2 microgram. The present invention only requires a trace amount of the liquid to be tested 40 to carry out the subsequent detection reaction. Compared with the conventional detection test strip, which requires a small amount of liquid to be tested. The liquid volume must be as high as 1 mg or more for detection. The present invention can indeed reduce the difficulty of inspection and greatly increase the efficiency of detecting samples.

The coloring agent 50 includes a mixture of reactive antibodies and colloidal gold, wherein the colloidal gold is previously combined with the reactive antibodies and retains a region where the reactive antibodies can bind to the protein to be detected. Colloidal gold is increasingly widely used in biomedical research fields, especially in medical testing. Colloidal gold is essentially a coating process in which macromolecules such as proteins are adsorbed to the surface of colloidal gold particles, that is, the negative charge on the surface of colloidal gold particles interacts with proteins, such as staphylococcal protein A, immunoglobulins, toxins, glycoproteins, enzymes, Non-covalent positively charged groups such as antibiotics and hormones form strong bonds due to electrostatic adsorption. Due to capillary action, the coloring agent 50 moves along the direction of the absorption area 222 of the adsorbent. When it moves to the area to be measured, the protein to be detected in the coloring agent 50 will generate antibodies with the reactive antibodies in the liquid to be measured 40 The specific binding reaction with the antigen gives rise to different colors.

Please refer to FIG. 7 , which shows another embodiment of the biological chip detection device of the present invention. This embodiment has partially the same structure as the previous embodiment, so the same elements are given the same symbols and their descriptions are omitted. The difference between this embodiment and the previous embodiment is that this embodiment includes two reaction membranes 21 , two diffusion membranes 22 and two filter membranes 24 . The upper cover 23 includes two first operating areas 231 and two second operating areas 232. The two first operating areas 231 are arranged adjacently, and the two filter membranes 24 correspond to the two first operating areas 231 respectively. The tie rods 212 of the reaction membranes 21 on both sides extend in opposite directions, thereby causing the two reaction membranes 21 to carry the diffusion membrane 22 and move in opposite directions.

The biological chip detection device of the present invention can detect multiple detection specimens at the same time. It only requires a trace amount of the liquid to be tested to react with the coloring agent. It can not only reduce the difficulty of inspection and greatly increase the number of detection samples, but also utilize the coloring agent. The peripheral diffusion flow method evenly combines and reacts with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the test results, which can effectively improve the accuracy of the test results as a whole. In addition, the biological chip detection device of the present invention also includes a filter membrane, which can filter out tissue debris or foreign matter in the test subject. Moreover, the reaction membrane of the present invention is movable and can be moved before dripping the coloring agent. First, move the reaction membrane from the first operating area to the second operating area so that the coloring agent can be dripped from the reaction opening to avoid affecting the color development of the final test result.

However, the above are only preferred embodiments of the present invention, and should not be used to limit the scope of the present invention. That is, simple equivalent changes and modifications may be made based on the patent scope and novel description content of the present invention. All are still within the scope of the patent of this invention. In addition, any embodiment or patentable scope of the present invention does not necessarily achieve all the purposes, advantages or features disclosed in the present invention. In addition, the abstract section and title are only used to assist in searching patent documents and are not intended to limit the scope of the invention. In addition, the terms "first" and "second" mentioned in this specification or the patent application are only used to name elements or to distinguish different embodiments or scopes, and are not used to limit the number of elements. upper or lower limit.

10: Base 11: Nose 12:Limit slot 20:Reaction module 21:Reaction membrane 22: Diffusion film 23: Upper cover 24:Filter membrane 40: Liquid to be tested 50: Coloring agent 211:Ontology 212:Tie rod 221: Liquid suction opening 222: Liquid suction area 231: First operating area 232: Second operating area 233:Drip hole 234:Reaction opening 235: concave part A:Latching post B:Latching hole

Figure 1 is a perspective view of an embodiment of the biowafer detection device of the present invention. FIG. 2 is an exploded view of the biological chip detection device of FIG. 1 . Figure 3 is a cross-sectional view of the biological chip detection device of Figure 1, in which the liquid to be tested is dripped from the drip hole. FIG. 4 is a schematic diagram of the reaction membrane and diffusion membrane of the biowafer detection device in FIG. 1 moving from the first operating area to the second operating area. FIG. 5 is a perspective view of the reaction membrane and diffusion membrane of the biowafer detection device in FIG. 4 located in the second operating area. FIG. 6 is a schematic diagram of the chromogen of the biowafer detection device in FIG. 5 after being dropped into the reaction membrane and then diffusing and moving toward the liquid to be tested. Figure 7 is a schematic diagram of another embodiment of the biological chip detection device of the present invention.

10: Base

11: Nose

12:Limit slot

20:Reaction module

21:Reaction membrane

22: Diffusion film

24:Filter membrane

211:Ontology

212:Tie rod

221: Liquid suction opening

222: Liquid suction area

231: First operating area

232: Second operating area

233:Drip hole

234:Reaction opening

A:Latching post

B:Latching hole

Claims (11)

A biological chip detection device, which includes: a base (10); and A reaction module (20) is provided on the base (10). The reaction module (20) includes a reaction membrane (21), a diffusion membrane (22), and a filter membrane (24) stacked from bottom to top. And an upper cover (23), the reaction film (21) is adjacent to the base (10), the diffusion film (22) has at least one liquid suction opening (221) and a suction hole located around the liquid suction opening (221). Liquid area (222), the upper cover (23) is provided on the base (10) and includes a first operating area (231) and a second operating area (232) adjacent to the first operating area (231), the The first operating area (231) of the upper cover (23) has a plurality of dripping holes (233) for dripping a test liquid (40), and the second operating area (232) has a plurality of dripping holes (233) for dripping a colorant. One reaction of (50) opens pores (234); The filter membrane (24) is disposed at a position corresponding to the first operating area (231) of the upper cover (23), and the reaction membrane (21) and the diffusion membrane (22) can be located at a position corresponding to the upper cover (23). 23) position movement between the first operating area (231) and the second operating area (232). The biological chip detection device according to claim 1, wherein the reaction film (21) includes a body (211) and a tie rod (212) connected to the body (211), and the diffusion film (22) is provided on the body (211). The biological chip detection device according to claim 2, wherein the size of the body (211) of the reaction membrane (21) is equal to the size of the diffusion membrane (22), and the reaction membrane (21) abuts the liquid absorbing opening. holes (221) and localized liquid-absorbing areas (222). The biological chip detection device as claimed in claim 2, wherein the side of the base (10) close to the upper cover (23) is provided with a flange (11) surrounding the edge of the base (10), and the reaction membrane (21) The body (211) is disposed in the area surrounded by the flange (11). A limiting groove (12) is formed on one side of the flange (11), and the tie rod (212) of the reaction membrane (21) extends through The limiting groove (12) is exposed on the base (10) and the upper cover (23). The biological chip detection device according to claim 3, wherein the pore diameter of the reaction opening (234) is larger than the pore diameter of the liquid absorption opening (221), and when the diffusion membrane (22) moves to correspond to the second operation When the liquid absorbing area (222) is in the position of the area (232), part of the liquid absorbing area (222) is exposed to the reaction opening (234). The biological chip detection device as claimed in claim 1, wherein the hole wall of each drip hole (233) is conical. The biological chip detection device according to claim 1, wherein the upper cover (23) is provided with a recess (235), which is provided in the first operating area (231), and the filter membrane (24) is provided and positioned in the recess (235) ) within. The biological chip detection device according to claim 1, wherein the drip holes (233) are arranged at intervals around the center of the first operating area (231). The biological chip detection device according to claim 1, wherein the reaction module (20) includes two reaction membranes (21), two diffusion membranes (22) and two filter membranes (24), and the upper cover (23) includes two first operating areas (231) and two second operating areas (232). The two first operating areas (231) are arranged adjacently. Each reaction membrane (21) and The diffusion film (22) moves between positions corresponding to one of the first operating areas (231) and one of the second operating areas (232). The biological chip detection device as claimed in claim 1, wherein each dripping hole (233) is for the test liquid (40) to drip into and penetrate through the filter membrane (24), the liquid suction opening (221) ) to the reaction film (21) to form an area to be measured, the reaction film (21) moves from the first operating area (231) to the second operating area (232), the reaction opening (234) and the suction The liquid opening (221) is for the coloring agent (50) to be dripped into the center position surrounding the area to be measured, and the coloring agent (50) is adsorbed and diffused toward the surroundings through the liquid absorption area (222). The areas to be detected react with each other and the final detection result is obtained. The biochip detection device of claim 1, wherein the chromogen (50) includes reactive antibodies and colloidal gold, and the capacity of the test liquid (40) is between 0.1 microgram and 0.2 microgram.

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