TW202402395A - Biochip detection device - Google Patents

Abstract

A biological chip detection device includes a base and a reaction module. The reaction module is arranged on the base and includes a reaction membrane, a diffusion membrane, at least one filter membrane and an upper cover stacked from bottom to top. The reaction membrane is adjacent to the base, and the diffusion membrane has at least one liquid suction opening and is located at the periphery of the liquid suction opening a liquid suction area of A second drop hole for the agent. The reaction membrane and the diffusion membrane are arranged at positions corresponding to the operation area of the upper cover, and the filter membrane can move between the operation area and the retreat area corresponding to the upper cover.

Description

Biochip detection device

The present invention relates to a biological chip detection device, and in particular to a technical field that can simultaneously detect a variety of different detection specimens and improve the accuracy of detection results.

Immunochromatography technology has played a very important role in today's medical system. It can be used to qualitatively or quantitatively measure changes in various biological indicators to provide laboratory personnel or medical personnel with information for disease diagnosis or drug treatment.

The vast majority of test strips currently on the market are qualitatively designed to allow the examiner to obtain the test results through visual inspection. Although a variety of different test items can be tested at the same time, the coloring agent needs to flow to the reactions of multiple liquids to be tested. zone, the colorant flows sideways in a single direction, and then flows to each reaction zone sequentially to react with the liquid to be tested. This method will cause the capacity of the colorant to flow to each reaction zone to gradually change. Rarely, due to the uneven distribution of the coloring agent, the liquid to be tested receives different coloring agents, resulting in inspection errors, or even inaccuracies in inspection. Therefore, how to use innovative detection device designs to simultaneously detect a variety of different detection items and improve detection accuracy is an urgent problem to be solved.

In addition, since the test specimen is taken from the human body or animal, in addition to the liquid part, sometimes the test specimen also contains some tissue debris, which may affect the test results.

In view of this, the object of the present invention is to provide a method that can detect multiple different test specimens at the same time. Only a trace amount of the test liquid can be reacted with the coloring agent, which can not only reduce the difficulty of inspection and greatly increase the detection time. The sample also utilizes the peripheral diffusion flow of the chromogen to evenly combine and react with all the liquids to be tested at the same time, which can effectively improve the accuracy of the test results.

An embodiment of the biochip detection device of the present invention includes a base and a reaction module. The reaction module is arranged on the base and includes a reaction membrane, a diffusion membrane, at least one filter membrane and an upper cover stacked from bottom to top. The reaction membrane is adjacent to the base, and the diffusion membrane has at least one liquid suction opening and is located around the liquid suction opening. A liquid suction area, the upper cover is arranged on the base and includes an operating area and a retreat area adjacent to the operating area. The operating area of the upper cover has a plurality of first dripping holes for dripping the liquid to be tested and for dripping to develop color. A second drop of agent into the hole. The reaction membrane and the diffusion membrane are arranged at a position corresponding to the operating area of the upper cover, and the filter membrane can move between a position corresponding to the operating area and the retreat area of the upper cover.

In another embodiment, the reaction module further includes at least one carrier, which is movably disposed between the base and the upper cover, and at least one filter membrane is disposed on the at least one carrier and moves with the at least one carrier.

In another embodiment, the carrier includes a body and a pull rod connected to the body, the body has a reaction opening, and the filter membrane is disposed on the body and corresponds to the reaction opening.

In another embodiment, a flange surrounding the edge of the upper cover is provided on one side of the upper cover close to the base. The body of the carrier is disposed in the area surrounded by the flange. A limiting groove is formed on one side of the flange through which the pull rod of the carrier extends. The limiting groove is exposed on the upper cover.

In another embodiment, the pore diameter of the reaction opening is larger than the pore diameter of the liquid-absorbing opening, and the liquid-absorbing area is partially exposed to the reaction opening.

In another embodiment, the wall of each drip hole is conical.

In another embodiment, the base is provided with a recess, the reaction membrane and the diffusion film are disposed and positioned in the recess, and the recess corresponds to the operating area of the upper cover.

In another embodiment, the first dripping hole is arranged at intervals outward from the center of the operating area, and the second dripping hole is located at the center of the first dripping hole.

In another embodiment, the size of the reaction film is equal to the size of the diffusion film, and the reaction film is in contact with the liquid-absorbing opening and part of the liquid-absorbing area.

In another embodiment, the reaction membrane group includes two filter membranes, and the upper cover includes two operating areas and two retreat areas. The two operating areas are arranged adjacently, and each filter membrane corresponds to one operating area and one evacuation area. Position movement between retreat zones.

In another embodiment, each first dripping hole is used for a liquid to be tested to drip into and penetrate through the filter membrane, reaction openings, and suction openings to the reaction membrane to form an area to be tested, and the filter membrane moves from the operating area to Moving to the retreat area, the second dripping hole and the liquid suction opening allow a coloring agent to be dripped into the center of the area around the area to be measured, and the coloring agent is adsorbed and diffused toward the surrounding area through the liquid suction area to flow to the area to be measured. The regions react with each other and obtain the final detection result.

In another embodiment, the coloring agent includes a reactive antibody and colloidal gold, and the capacity of the test solution is between 0.1 microgram and 0.2 microgram.

The biological chip detection device of the present invention can detect multiple detection specimens at the same time. It only requires a trace amount of the liquid to be tested to react with the coloring agent. It can not only reduce the difficulty of inspection and greatly increase the number of detection samples, but also utilize the coloring agent. The peripheral diffusion flow method evenly combines and reacts with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the test results, which can effectively improve the accuracy of the test results as a whole. In addition, the biological chip detection device of the present invention also includes a filter membrane, which can filter out tissue debris or foreign matter in the test subject, and before dripping the chromogenic agent, the filter membrane can be moved from the operating position. to the retreat position to avoid affecting the color development of the final test result.

Please refer to FIG. 1, FIG. 2, and FIG. 3, which is an embodiment of the biochip detection device of the present invention. The biological chip detection device of the present invention is applied to immunochromatography technology. The biological chip detection device of this embodiment includes a base 10 and a reaction module 20 . The reaction module 20 is arranged on the base 10 .

The reaction module 20 includes a reaction membrane 21, a diffusion membrane 22, a filter membrane 24 and an upper cover 23 stacked from bottom to top. The reaction film 21 is adjacent to the base 10 . The reaction membrane 21 can be a nitro-cellulose membrane, a PVDF membrane (polyvinylidone (PVDF) membrane), a nylon membrane, etc., and is used to adsorb the protein to be detected in the test liquid 40 . The diffusion film 22 has at least one liquid suction opening 221 and a liquid suction area 222 located around the liquid suction opening 221. The upper cover 23 is engaged with the base 10 through the engagement post A and the engagement hole B provided in the base 10. , and the upper cover 23 is provided with a flange 235 on the surface close to the base 10, so that when the upper cover 23 is engaged with the base 10, a receiving space is formed between the upper cover 23 and the base 10, and the reaction membrane 21, the diffusion membrane 22 and the filter membrane are 24 are arranged in the accommodation space within the flange 235.

The upper cover 23 is rectangular and includes an operating area 231 and a retreat area 232 . The upper cover 23 forms a plurality of first dripping holes 233 and a second dripping hole 234 in the operating area 231 . The first dripping holes 233 are arranged at intervals outward from the center of the operating area 231 , and the second dripping holes 234 are located at the center of the operating area 231 and surrounded by a plurality of first dripping holes 233 .

The reaction module 20 further includes at least one carrier 25, which is movably disposed between the base 10 and the upper cover 23. The filter membrane 24 is disposed on the carrier 25 and moves with the carrier 25. The carrier 25 of this embodiment includes a body 251 and a pull rod 252 connected to the body 251. The body 251 has a reaction opening 253, and the filter membrane 24 is disposed on the body 251 and corresponds to the reaction opening 253. A limiting groove 236 is provided on one side of the flange 235 of the upper cover 23 . The pull rod 252 extends through the limiting groove 236 and is exposed on the upper cover 23 . The user pulls the pull rod 252 to move the carrier 25 between the upper cover 23 and the base 10 . The filter membrane 24 can filter the biological tissue debris in the liquid to be tested 40 to prevent the biological tissue debris from affecting the detection results.

The base 10 is provided with a recess 11 . The reaction film 21 and the diffusion film 22 are arranged and positioned in the recess 11 . The recess 11 corresponds to the operating area 231 of the upper cover 23 . The reaction film 21 and the diffusion film 22 form a configuration fit with the recess 11 , whereby the reaction film 21 is positioned on the base 10 .

As shown in FIG. 3 , when the body 251 of the carrier 25 moves to the operating position, the reaction opening 253 and the liquid suction opening 221 are connected. The size of the reaction opening 253 is larger than the pore diameter of the liquid suction opening 221 , and the liquid suction area 222 is partially exposed to the reaction opening 253 , so that the liquid to be tested 40 can filter out tissue debris through the filter membrane 24 and then enter the reaction membrane 21 . The material of the upper cover 23 is transparent, which helps the detector to directly observe the reaction process and status of the detection. The size of the reaction film 21 is substantially equal to the size of the diffusion film 22. The reaction film 21 is in contact with the liquid suction opening 221 and the liquid suction area 222. As long as the reaction film 21 can cover the range of the liquid suction opening 221, it can ensure the desired dripping. It suffices that the liquid (such as the liquid to be tested, the coloring agent and the cleaning liquid, etc.) can fall into the reaction film 21 . The diffusion membrane 22 of this embodiment has a liquid-absorbing opening 221, and the liquid-absorbing opening 221 is circular.

As shown in Figure 3, the hole wall of each first dropping hole 233 is conical, and the pore diameter on the side close to the reaction membrane 21 is smaller than the pore diameter on the side far away from the reaction membrane 21. For example, when the liquid to be tested 40 enters the first dropping hole 233, the first dripping hole 233 uses the conical hole wall to gather the liquid to be tested 40 at a specific position to avoid overflow, and wait for the liquid to be tested 40 to gradually spread in the reaction membrane 21. After the liquid to be tested 40 is dripped from the first dropping hole 233, the liquid to be tested 40 will penetrate through the filter membrane 24 and then pass through the reaction opening 253 and the liquid suction opening 221 to the reaction membrane 21 to form a region to be measured.

Then, as shown in FIGS. 4 and 5 , the carrier 25 is pulled to move the filter membrane 24 from the operating area 231 to the retreat area 232 . At this time, the inspector can directly view multiple areas to be tested formed on the reaction membrane 21 . The sides of the first drip hole 233 may have different drip marks, such as numbers, patterns, or English letters. In this embodiment, the numbers 1 and 2 are marked next to the first drip hole 233. ,3,4,5,6,7, as shown in Figure 4. The drip mark corresponds to a piece of test data of the liquid to be tested 40. The test data includes relevant information such as subject information, specimens, and test items. In this way, the examiner can clearly identify each corresponding test item through the drip mark. Testing data can effectively prevent inspectors from making mistakes. Each first dripping hole 233 is for dripping a liquid to be tested 40 , which can be body fluid or blood. Here, four first dripping holes 233 are used as an example. Each first dripping hole 233 is used for dripping. The test liquids 40 dropped into 233 are all different and belong to different test data. The subject data and the specimen are different, but the test items can be the same or different, and can be adjusted appropriately according to the test requirements.

Then, as shown in FIG. 6 , the toner 50 is dropped from the second dropping hole 234 . As shown in FIG. 7 , the coloring agent 50 is dripped into the center position surrounded by multiple areas to be measured through the liquid suction opening 221 , and the coloring agent 50 is adsorbed by the liquid suction area 222 and diffuses toward the surroundings to flow to the multiple areas to be measured. to react with each other. Among them, the liquid absorbing area 222 is located around the liquid absorbing opening 221, so the coloring agent 50 is subject to the capillary action of the liquid absorbing area 222, and the coloring agent 50 flows uniformly and simultaneously toward multiple areas to be measured at a free angle until the liquid absorbing area. 222, so that all areas to be measured can receive the coloring agent 50 evenly, so that the coloring agent 50 and the liquid to be measured 40 can combine and react. It is worth noting that the capacity of the liquid to be tested 40 is between 0.1 microgram and 0.2 microgram. The present invention only requires a trace amount of the liquid to be tested 40 to carry out the subsequent detection reaction. Compared with the conventional detection test strip, which requires a small amount of liquid to be tested. The liquid volume must be as high as 1 mg or more for detection. The present invention can indeed reduce the difficulty of inspection and greatly increase the efficiency of detecting samples.

The coloring agent 50 includes a mixture of reactive antibodies and colloidal gold, wherein the colloidal gold is previously combined with the reactive antibodies and retains a region where the reactive antibodies can bind to the protein to be detected. Colloidal gold is increasingly widely used in biomedical research fields, especially in medical testing. Colloidal gold is essentially a coating process in which macromolecules such as proteins are adsorbed to the surface of colloidal gold particles, that is, the negative charge on the surface of colloidal gold particles interacts with proteins, such as staphylococcal protein A, immunoglobulins, toxins, glycoproteins, enzymes, Non-covalent positively charged groups such as antibiotics and hormones form strong bonds due to electrostatic adsorption. Due to capillary action, the coloring agent 50 moves along the direction of the absorption area 222 of the adsorbent. When it moves to the area to be measured, the protein to be detected in the coloring agent 50 will generate antibodies with the reactive antibodies in the liquid to be measured 40 The specific binding reaction with the antigen gives rise to different colors.

Please refer to FIG. 8 , which shows another embodiment of the biological chip detection device of the present invention. This embodiment has partially the same structure as the previous embodiment, so the same elements are given the same symbols and their descriptions are omitted. The difference between this embodiment and the previous embodiment is that this embodiment includes two filter membranes 24 and two carriers 25 . The upper cover 23 includes two operating areas 231 and two retreat areas 232. The two operating areas 231 are arranged adjacently, and each filter membrane 24 moves to a position corresponding to one operating area 231 and one retreat area 232. The tie rods 252 of the carriers 25 on both sides extend in opposite directions, thereby causing the two carriers 25 to carry the filter membrane 24 and move in opposite directions.

The biological chip detection device of the present invention can detect multiple detection specimens at the same time. It only requires a trace amount of the liquid to be tested to react with the coloring agent. It can not only reduce the difficulty of inspection and greatly increase the number of detection samples, but also utilize the coloring agent. The peripheral diffusion flow method evenly combines and reacts with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the test results, which can effectively improve the accuracy of the test results as a whole. In addition, the biological chip detection device of the present invention also includes a filter membrane, which can filter out tissue debris or foreign matter in the test subject, and before dripping the chromogenic agent, the filter membrane can be moved from the operating position. to the retreat position to avoid affecting the color development of the final test result.

However, the above are only preferred embodiments of the present invention, and should not be used to limit the scope of the present invention. That is, simple equivalent changes and modifications may be made based on the patent scope and novel description content of the present invention. All are still within the scope of the patent of this invention. In addition, any embodiment or patentable scope of the present invention does not necessarily achieve all the purposes, advantages or features disclosed in the present invention. In addition, the abstract section and title are only used to assist in searching patent documents and are not intended to limit the scope of the invention. In addition, terms such as "first" and "second" mentioned in this specification or the patent application are only used to name elements or distinguish different embodiments or scopes, and are not used to limit the number of elements. upper or lower limit.

10: Base 11: concave part 20:Reaction module 21:Reaction membrane 22: Diffusion film 23: Upper cover 24:Filter membrane 25: Carrier 40: Liquid to be tested 50: Coloring agent 221: Liquid suction opening 222: Liquid suction area 231: Operation area 232:Retreat area 233:The first drop into the hole 234: Second drip hole 235: Nosed edge 236:Limit slot 251:Ontology 252:Tie rod 253:Reaction opening A:Latching post B:Latching hole

Figure 1 is a perspective view of an embodiment of the biowafer detection device of the present invention. FIG. 2 is an exploded view of the biological chip detection device of FIG. 1 . Figure 3 is a cross-sectional view of the biological chip detection device of Figure 1, in which the liquid to be tested is dripped from the first dripping hole. FIG. 4 is a schematic diagram of the biological chip detection device in FIG. 1 in use. FIG. 5 is a perspective view of the carrier of the biowafer detection device in FIG. 4 moving from the operating position to the retreat position at the upper cover. FIG. 6 is a cross-sectional view of the biowafer detection device of FIG. 4 , in which the coloring agent is dripped from the second dripping hole. FIG. 7 is a schematic diagram of the chromogen of the biowafer detection device in FIG. 6 being dropped into the reaction membrane and then diffusing and moving toward the liquid to be tested. Figure 8 is a schematic diagram of another embodiment of the biological chip detection device of the present invention.

10: Base

11: concave part

20:Reaction module

21:Reaction membrane

22: Diffusion film

23: Upper cover

24:Filter membrane

25: Carrier

221: Liquid suction opening

222: Liquid suction area

231: Operation area

232:Retreat area

233:The first drop into the hole

234: Second drip hole

235: Nosed edge

236:Limit slot

251:Ontology

252:Tie rod

253:Reaction opening

A:Latching post

B:Latching hole

Claims (12)

A biological chip detection device, which includes: a base (10); and A reaction module (20) is provided on the base (10). The reaction module (20) includes a reaction membrane (21), a diffusion membrane (22), and at least one filter membrane (24) stacked from bottom to top. ) and an upper cover (23). The reaction membrane (21) is adjacent to the base (10). The diffusion membrane (22) has at least one liquid suction opening (221) and a liquid suction opening (221) located around the periphery of the liquid suction opening (221). Liquid suction area (222), the upper cover (23) is provided on the base (10) and includes an operating area (231) and a retreat area (232) adjacent to the operating area (231), the upper cover (23) The operating area (231) has a plurality of first dropping holes (233) for dropping a liquid to be tested (40) and a second dropping hole (234) for dropping a coloring agent (50); The reaction membrane (21) and the diffusion membrane (22) are arranged at a position corresponding to the operating area (231) of the upper cover (23), and the filter membrane (24) can be located corresponding to the upper cover (23). The position between the operation area (231) and the retreat area (232) is moved. The biological chip detection device according to claim 1, wherein the reaction module (20) further includes at least one carrier (25), which is movably disposed between the base (10) and the upper cover (23), the At least one filter membrane (24) is disposed on the at least one carrier (25) and moves with the at least one carrier (25). The biological chip detection device according to claim 2, wherein the carrier (25) includes a body (251) and a tie rod (252) connected to the body (251), and the body (251) has a reaction opening ( 253), the filter membrane (24) is disposed on the body (251) and corresponds to the reaction opening (253). The biological chip detection device as claimed in claim 3, wherein the side of the upper cover (23) close to the base (10) is provided with a flange (235) surrounding the edge of the upper cover (23), and the side of the carrier (25) The body (251) is disposed in the area surrounded by the flange (235). A limiting groove (236) is formed on one side of the flange (235), and the tie rod (252) of the carrier (25) extends through the limiting groove. slot (236) and exposed on the upper cover (23). The biological chip detection device according to claim 3, wherein the pore diameter of the reaction opening (253) is larger than the pore diameter of the liquid suction opening (221), and the liquid suction area (222) is partially exposed to the reaction opening (221). 253). The biological chip detection device according to claim 1, wherein the hole wall of each first drip hole (233) is conical. The biological chip detection device as claimed in claim 1, wherein the base (10) is provided with a recess (11), the reaction film (21) and the diffusion film (22) are provided and positioned in the recess (11), and the The recess (11) corresponds to the operating area (231) of the upper cover (23). The biological chip detection device according to claim 1, wherein the first drip holes (233) are arranged at intervals outward from the center of the operating area (231), and the second drip holes (234) are located at The center position of the first drip holes (233). The biological chip detection device according to claim 1, wherein the size of the reaction film (21) is equal to the size of the diffusion film (22), and the reaction film (21) abuts the liquid absorption opening (221) and the local The suction area (222). The biological chip detection device as claimed in claim 1, wherein the reaction membrane group (20) includes two filter membranes (24), and the upper cover (23) includes two of the operation areas (231) and two of the retreat areas ( 232), the two operating areas (231) are arranged adjacently, and each filter membrane (24) moves to a position corresponding to one of the operating areas (231) and one of the retreat areas (232). The biological chip detection device as claimed in claim 1, wherein each first drip hole (233) is for a test liquid (40) to drip into and penetrate through the filter membrane (24), the reaction opening ( 253), the liquid suction opening (221) to the reaction membrane (21) form an area to be measured, the filter membrane (24) moves from the operating area (231) to the retreat area (232), the second drop The inlet hole (234) and the liquid suction opening (221) are for a coloring agent (50) to be dripped into the center position surrounding the area to be measured, and the coloring agent (50) is transferred through the liquid suction area (222). 50) The adsorption diffuses toward the surrounding area and flows to the area to be measured to form a reaction with each other and obtain the final detection result. The biochip detection device of claim 11, wherein the chromogen (50) includes reactive antibodies and colloidal gold, and the capacity of the test liquid (40) is between 0.1 microgram and 0.2 microgram.

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