TWM633777U - Biochip detection device - Google Patents

Abstract

A biological chip detection device includes a base, a reaction module and at least one sliding cover. The reaction module is attached to the base, and the reaction module includes a reaction membrane, a diffusion membrane and an upper cover stacked from bottom to top, the reaction membrane is adjacent to the base, and the diffusion membrane has at least one liquid-absorbing opening and a liquid suction area, the upper cover has a reaction opening and a pair of sliding grooves, and the reaction opening and the liquid suction opening are in communication. At least one sliding cover is arranged on the reaction module, the sliding cover has a body and a plurality of dropping holes on the body, the body is slidably arranged in the pair of chutes of the upper cover, and the positions of the dropping holes correspond to the opening of the reaction. The pores are connected to each other through the reaction membrane.

Description

Biochip Detection Device

The invention relates to a biochip detection device, in particular to a technical field that can simultaneously detect a variety of different detection samples and improve the accuracy of detection results.

Immunochromatographic technology has played a very important role in today's medical system. It can be used to qualitatively or quantitatively measure the changes of various biological indicators, so as to provide references for inspectors or medical personnel for disease diagnosis or drug treatment.

At present, most of the test strips on the market are qualitatively designed to allow the tester to know the test results visually, such as the "Upper and Lower Gastrointestinal Cancer Screening Test Set and Its Sampling Solution and Sampling" in Patent No. I561820 of the Republic of China method", but the test strips in the previous case can only detect a single item and cannot detect a variety of different test items at the same time. Another example is the "multi-sample rapid detection test strip device (1)" of the Republic of China Patent No. M554567. It is a single-direction lateral flow, and then flows to each reaction area in sequence to react with the liquid to be tested. This method will cause the capacity of the coloring agent to flow to each reaction area to decrease gradually, because the coloring agent The non-uniform distribution makes the color-forming agent received by the liquid to be tested be different, resulting in inspection errors and even inaccurate inspections. Therefore, how to simultaneously detect a variety of different detection items and improve detection accuracy through an innovative detection device design is an urgent problem to be solved.

In addition, because the test sample is taken from the human body or animal, in addition to the liquid part, sometimes the test sample also contains some debris of the tissue, which may affect the test result.

In view of this, the purpose of this creation is to provide a method that can detect multiple different test objects at the same time, and only need a small amount of the liquid to be tested to react with the coloring agent, which can not only reduce the difficulty of sampling and greatly increase the detection The sample also uses the peripheral diffusion flow mode of the coloring agent to uniformly combine and react with all the liquids to be tested at the same time, which can effectively improve the accuracy of the test results.

An embodiment of the biochip testing device of the present invention includes a base, a reaction module and at least one sliding cover. The reaction module is attached to the base. The reaction module includes a reaction membrane, a diffusion membrane and an upper cover stacked from bottom to top. The reaction membrane is adjacent to the base. The diffusion membrane has at least one liquid-absorbing opening and is located around the liquid-absorbing opening A liquid suction area, the upper cover has a reaction opening and a pair of slide grooves, and the reaction opening is connected with the liquid suction opening. At least one sliding cover is arranged on the reaction module. The sliding cover has a body and a plurality of drip holes on the body. The body is slidably arranged in the pair of slide grooves of the upper cover, and the position of the drip holes corresponds to the reaction opening. pores, and communicate with each other in the reaction membrane.

In another embodiment, the reaction module further includes at least one filter membrane disposed between the upper cover and the sliding cover, and corresponding to the dropping holes of the sliding cover and the reaction openings of the upper cover.

In another embodiment, the body of the sliding cover is provided with a first recess, and the filter membrane is disposed and positioned in the first recess.

In another embodiment, the wall of each drip hole is conical.

In another embodiment, the base is provided with a second recess, and the reaction film is disposed and positioned in the second recess.

In another embodiment, the body has a drop-in area, and the drip-in holes are located in the drop-in area, and are arranged around the center of the drop-in area outward at intervals.

In another embodiment, the diameter of the reaction opening is larger than that of the liquid-absorbing opening, and the liquid-absorbing area is partially exposed to the reaction opening.

In another embodiment, the size of the reaction membrane is smaller than the size of the diffusion membrane, and the reaction membrane interferes with the liquid absorption opening and the local liquid absorption area.

In another embodiment, the diffusion membrane has two liquid absorption openings, and the size of the reaction opening is greater than the sum of the dimensions of the two liquid absorption openings.

In another embodiment, the two sliding covers are separated from each other and slidably disposed in the pair of sliding grooves of the upper cover.

In another embodiment, the dropping holes of the sliding cover are divided into two groups, and the dropping holes of the two groups respectively correspond to two liquid suction openings.

In another embodiment, the diffusion film includes a first half-diffusion film and a second half-diffusion film, and the first half-diffusion film and the second half-diffusion film are partially overlapped and connected to opposite sides of the reaction film, and the first The half-diffusion film and the second half-diffusion film are separated by a predetermined distance to form liquid-absorbing openings.

In another embodiment, each drop-in hole is for a liquid to be tested to drop into and penetrate through the reaction opening, absorb the liquid through the opening to the reaction membrane to form a region to be tested, and move away from the reaction mold on the body When combined, the reaction opening and the liquid-absorbing opening are used to allow a color-forming agent to drop into the center around the area to be tested, and the color-forming agent is adsorbed by the liquid-absorbing area to diffuse and flow to the area to be tested to form a reaction with each other. , and get the final test result.

In another embodiment, the coloring agent includes reactive antibodies and colloidal gold, and the volume of the test solution is between 0.1 micrograms and 0.2 micrograms.

The biological chip detection device created by this invention can detect multiple detection samples at the same time, and only needs a small amount of the liquid to be tested to react with the coloring agent, which not only reduces the difficulty of sampling and greatly increases the number of detection samples, but also uses the coloring agent The surrounding diffusion flow method uniformly combines and reacts with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the detection results, which can effectively improve the accuracy of the detection results as a whole. In addition, the biochip detection device of the present invention also includes a filter membrane arranged between the upper cover and the sliding cover, which can filter out tissue debris or foreign matter in the test sample, so as to avoid affecting the color development of the final test result.

Please refer to FIG. 1 , FIG. 2 , FIG. 3 , FIG. 4A and FIG. 4B , which are an embodiment of the biochip detection device of the present invention. The biological chip detection device of the invention is applied to immunochromatography technology. The biochip testing device of this embodiment includes a base 10 , a reaction module 20 and at least one sliding cover 30 . The reaction module 20 is disposed on the base 10 , and at least one sliding cover 30 is slidably disposed on the reaction module 20 .

The reaction module 20 includes a reaction film 21 , a diffusion film 22 and a cover 23 stacked from bottom to top. The upper cover 23 has an engaging column A, and the base 10 has an engaging hole B. The engaging column A is engaged in the engaging hole B, so that the reaction film 21 is adjacent to the base 10 . The reaction membrane 21 can be a nitrocellulose membrane, a PVDF membrane [polyvinylidone (PVDF) membrane], a nylon membrane, etc., and is used to adsorb the protein to be detected in the test solution 40 . The diffusion film 22 has at least one liquid absorption opening 221 and a liquid absorption area 222 located around the liquid absorption opening 221 . The upper cover 23 has a reaction opening 231 , a pair of slide grooves 232 and a pair of stop protrusions 233 . The reaction opening 231 communicates with the liquid absorption opening 221 . The size of the reaction opening 231 is larger than the diameter of the liquid absorption opening 221 , and the liquid absorption area 222 is partially exposed to the reaction opening 231 , which helps the tester to directly observe the reaction process and status of the test. The sliding slots 232 are disposed on opposite sides of the upper cover 23 for installing the sliding cover 30 described later. The stop protrusions 233 are located at opposite ends of the upper cover 23 respectively, and are used to stop the slide cover 30 described later, so as to limit the movement limit position of the slide cover 30 .

The size of the reaction film 21 is substantially equal to the size of the diffusion film 22. The reaction film 21 is in contact with the liquid absorption opening 221 and the local liquid absorption area 222. As long as the reaction film 21 can cover the scope of the liquid absorption opening 221, it can ensure that the liquid to be dripped Liquids (such as liquids to be tested, colorants, cleaning liquids, etc.) can fall into the reaction membrane 21 . The diffusion membrane 22 of this embodiment has two liquid-absorbing openings 221 , and the liquid-absorbing openings 221 are circular. The upper cover 23 of this embodiment has a reaction opening 231 , the reaction opening 231 is rectangular, and the length and width of the reaction opening 231 are larger than the diameter of the liquid absorption opening 221 .

The reaction module 20 of this embodiment further includes two filter membranes 24 corresponding to the two liquid-absorbing openings 221 of the diffusion membrane 22 . The filter membrane 24 can filter the biological tissue debris in the liquid to be tested 40, so as to prevent the biological tissue debris from affecting the detection result.

The biochip detection device of this embodiment has two sliding covers 30 disposed on the reaction module 20 , respectively corresponding to the two liquid-absorbing openings 221 of the two filter membranes 24 and the diffusion membrane 22 . Each sliding cover 30 has a main body 31 , a stop groove 32 disposed at one end of the sliding cover 30 , and a plurality of drip holes 33 located on the main body 31 . The main body 31 is slidably disposed in the pair of sliding slots 232 of the upper cover 23 , and the position of the dropping hole 33 corresponds to the reaction opening 231 , and communicates with the reaction membrane 21 . The body 31 of each sliding cover 30 has a first concave portion 311 on the surface close to the upper cover 23 , and the filter membrane 24 is disposed and positioned in the first concave portion 311 . The movement stroke of the sliding cover 30 is limited by the stopping groove 32 of the sliding cover 30 being stopped by the stopping projection 233 of the sliding cover 30 . The body 31 of this embodiment has a drop-in area F, and the drop-in holes 33 are located in the drop-in area F, and are arranged around the center of the drop-in area F outward at intervals. Each sliding cover 30 in this embodiment has four drip holes 33 , and the four drip holes 33 are arranged around the center of the drip area F at equiangular distances. In order to detect different test data at the same time, the sides of the drip holes 33 can have different drip marks, such as numbers, patterns or English letters. The infusion mark 34 is a test data corresponding to the liquid to be tested 40, and the test data includes relevant information such as the subject data, the specimen and the test items, so that the tester can clearly identify each corresponding liquid through the infusion mark. The testing data can effectively prevent the inspectors from making mistakes. Each drop-in hole 33 is for a liquid to be tested 40 to drop into, and the liquid to be tested 40 can be body fluid or blood. Here, four drop-in holes 33 are used as an example to illustrate, and each drop-in hole 33 drips the liquid to be tested. The testing liquids 40 are all different and belong to different testing data, wherein the data of the subject and the sample are different, but the testing items can be the same or different, and can be properly adjusted according to the testing requirements.

As shown in Figure 4B, the hole wall of each drop-in hole 33 is conical, and the aperture near the reaction membrane 21 side is smaller than the aperture away from the reaction membrane 21 side. The inlet hole 33 gathers the liquid to be tested 40 at a specific position to avoid overflow through the conical hole wall, and waits for the liquid to be tested 40 to gradually diffuse in the reaction membrane 21 .

The base 10 of this embodiment is provided with a second concave portion 11 , and the reaction film 21 is disposed and positioned in the second concave portion 11 . The second recess 11 in this embodiment is rectangular, and the reaction film 21 forms a configuration fit with the second recess 11 , so that the reaction film 21 is positioned on the base 10 .

As shown in Figure 5, since the position of the drop-in hole 33 corresponds to the reaction opening 231 and is connected to each other, after the liquid to be tested 40 is dropped from the drop-in hole 33, the liquid to be tested 40 will permeate through the reaction opening 231, The liquid suction hole 221 reaches the reaction membrane 21 to form a region to be tested. At this time, the two slide covers 30 can be moved to both sides along the chute 232 to move away from the reaction module 20 to reveal the region to be tested of the reaction membrane 21 , at this time, the inspector can directly observe the eight regions to be tested formed on the reaction film 21 , such as a group of regions 1 , 2 , 3 , 4 and another group of regions 5 , 6 , 7 , 8 as shown in FIG. 5 .

The reaction opening 231 and the liquid absorption opening 221 are for the color forming agent 50 to drip into the central position surrounded by the areas 1, 2, 3, 4 and drop into the central position surrounded by the areas 5, 6, 7, 8, The coloring agent 50 is adsorbed and diffused toward the surroundings through the liquid absorption area 222 to flow to areas 1, 2, 3, 4 and areas 5, 6, 7, 8 to form reactions with each other. Wherein, the liquid-absorbing area 222 is located around the liquid-absorbing opening 221, so the coloring agent 50 is subjected to the capillary action of the liquid-absorbing area 222, and the coloring agent 50 is uniformly and simultaneously directed to areas 1, 2, 3, 4 and 5 at a free angle. , 6, 7, and 8 areas flow until the liquid-absorbing area 222, so that all areas 1, 2, 3, 4 and areas 5, 6, 7, and 8 can receive the coloring agent 50 evenly, so that the coloring agent 50 and the coloring agent 50 The test solution 40 of the colorant 50 is combined and reacted. It is worth noting that the capacity of the test solution 40 is between 0.1 micrograms and 0.2 micrograms. This invention only needs a small amount of the test solution 40 to carry out subsequent detection reactions. The liquid capacity is as high as 1 mg or more to detect. This creation can indeed reduce the difficulty of sampling and greatly increase the efficacy of testing samples.

The coloring agent 50 is composed of a mixture of reactive antibody and colloidal gold, wherein the colloidal gold is combined with the reactive antibody in advance and retains the region where the reactive antibody can bind to the protein to be detected. Colloidal gold has been increasingly widely used in the fields of biomedical research, especially in medical testing. Colloidal gold is essentially a coating process in which polymers such as proteins are adsorbed to the surface of colloidal gold particles, that is, the negative charges on the surface of colloidal gold particles and proteins, such as staphylococcal protein A, immunoglobulins, toxins, glycoproteins, enzymes, Non-covalent positively charged groups such as antibiotics and hormones form strong bonds due to electrostatic adsorption. Due to capillary action, the coloring agent 50 moves along the direction of the absorbent region 222 of the adsorbent. The protein will react with the reactive antibody in the test solution 40 to produce a specific binding reaction between the antibody and the antigen, and present different colors.

Please refer to FIG. 6 , FIG. 7 and FIG. 8 , which represent another embodiment of the biochip detection device of the present invention. This embodiment has partly the same structure as the previous embodiment, so the same elements are given the same symbols and their descriptions are omitted. The difference between this embodiment and the previous embodiment is that this embodiment includes a filter membrane 24 and a sliding cover 30 . The sliding cover 30 includes a body 31 and a plurality of drip holes 33 disposed on the body 31 . The plurality of drop-in holes 33 in this embodiment are divided into two groups, and the drop-in holes 33 of each group are equidistantly arranged around a central position, and the drop-in holes 33 of each group correspond to the diffusion film 22 A liquid suction opening 221.

After the liquid to be tested 40 is dripped into the drip hole 33, the liquid to be tested 40 will permeate through the reaction opening 231 and the liquid suction opening 221 to the reaction membrane 21 to form a region to be tested. At this time, the slide cover 30 can be moved along the The chute 232 moves to the left or the right to reveal the area to be tested of the reaction film 21. At this time, the inspector can directly watch the four areas to be tested formed on the reaction film 21, as shown in Figure 8, a group of areas 1, 2, 3, 4 and another set of areas 5, 6, 7, 8. The coloring agent 50 is dropped into the central positions surrounded by the areas 1, 2, 3, 4 and into the central positions surrounded by the areas 5, 6, 7, 8, and the coloring agent 50 is adsorbed by the liquid absorption area 222 Diffusion flows towards the surroundings to areas 1, 2, 3, 4 and areas 5, 6, 7, 8 to form reactions with each other.

Please refer to FIG. 9 , which shows yet another embodiment of the biochip detection device of the present invention. This embodiment has partly the same structure as the previous embodiment, so the same elements are given the same symbols and their descriptions are omitted. The difference between this embodiment and the previous embodiment is that the diffusion film 22 includes a first half-diffusion film 223 and a second half-diffusion film 224, and the first half-diffusion film 223 and the second half-diffusion film 224 are partially overlapped and connected to the reaction film respectively. 21 and a predetermined distance between the first half-diffusion film 223 and the second half-diffusion film 224 to form liquid-absorbing openings 221 . The diffusion membrane 22 of this embodiment is not provided with a round-hole-shaped liquid-absorbing opening 221 , but divides the diffusion membrane 22 into two halves, a first half-diffusion membrane 223 and a second half-diffusion membrane 224 . After the liquid to be tested 40 is dripped from the dripping hole 33, the coloring agent 50 is dripped into the central position surrounded by the area to be tested in the eight areas to be tested, and the first half of the left and right halves The diffusion film 223 and the second semi-diffusion film 224 absorb the coloring agent 50 and diffuse toward the surroundings and flow to each detection area to form a reaction with each other.

The biological chip detection device created by this invention can detect multiple detection samples at the same time, and only needs a small amount of the liquid to be tested to react with the coloring agent, which not only reduces the difficulty of sampling and greatly increases the number of detection samples, but also uses the coloring agent The surrounding diffusion flow method uniformly combines and reacts with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the detection results, which can effectively improve the accuracy of the detection results as a whole. In addition, the biochip detection device of the present invention also includes a filter membrane arranged between the upper cover and the sliding cover, which can filter out tissue debris or foreign matter in the test sample, so as to avoid affecting the color development of the final test result.

However, the above is only a preferred embodiment of this creation, and should not limit the scope of implementation of this creation, that is, all simple equivalent changes and modifications made according to the patent scope of this creation and the content of the new description, All still belong to the scope covered by this creation patent. In addition, any embodiment or patent scope of this creation does not need to achieve all the purposes or advantages or characteristics disclosed in this creation. In addition, the abstract part and the title are only used to assist in the search of patent documents, and are not used to limit the scope of rights of this creation. In addition, terms such as "first" and "second" mentioned in this specification or the scope of the patent application are only used to name elements (elements) or to distinguish different embodiments or ranges, and are not used to limit the number of elements. upper or lower limit.

1, 2, 3, 4, 5, 6, 7, 8: areas to be tested 10: Base 11: Second recess 20: React Module 21: Reaction film 22: Diffusion film 23: Upper cover 24: filter membrane 30: slide cover 31: Ontology 32: Stop groove 33: Drip hole 34: drip mark 40: liquid to be tested 50: coloring agent 221: suction hole 222: suction area 223: The first semi-diffusion film 224: second half-diffusion film 231:Reaction opening 232: Chute 233: Stop bump 311: the first recess A: snap column B: Fastening hole F: drop into area

FIG. 1 is a perspective view of an embodiment of a biochip detection device of the present invention. FIG. 2 is a partially exploded view of the biochip detection device of FIG. 1 . FIG. 3 is an exploded view of the biochip detection device in FIG. 1 . FIG. 4A is a cross-sectional view of the biowafer detection device of FIG. 1 . FIG. 4B is a partially enlarged view of FIG. 4A. FIG. 5 is a schematic diagram of the color-forming agent dripping into the reaction membrane through the reaction opening and the liquid absorption opening after the sliding cover of the biochip detection device in FIG. 1 is slid away and moving toward the liquid to be tested. Fig. 6 is a perspective view of another embodiment of the biowafer detection device of the present invention. FIG. 7 is a partially exploded view of the biowafer detection device of FIG. 6 . FIG. 8 is a schematic diagram showing that the coloring agent drops into the reaction membrane through the reaction opening and the liquid absorption opening after the sliding cover of the biochip detection device in FIG. Fig. 9 is a perspective view of yet another embodiment of the biowafer detection device of the present invention.

10: Base

11: Second recess

20: React Module

21: Reaction film

22: Diffusion film

23: Upper cover

24: filter membrane

30: slide cover

31: Ontology

32: Stop groove

33: Drip hole

221: suction hole

222: suction area

231:Reaction opening

232: Chute

233: Stop bump

311: the first recess

Claims (14)

A biochip detection device comprising: a base (10); A reaction module (20), which is attached to the base (10), the reaction module (20) includes a reaction film (21), a diffusion film (22) and an upper cover (23) stacked from bottom to top , the reaction membrane (21) is adjacent to the base (10), the diffusion membrane (22) has at least one liquid absorption opening (221) and a liquid absorption area (222) located around the liquid absorption opening (221), The upper cover (23) has a reaction opening (231) and a pair of slide grooves (232), and the reaction opening (231) is in communication with the liquid suction opening (221); and At least one slide cover (30) is arranged on the reaction module (20), the slide cover (30) has a body (31) and a plurality of drip holes (33) on the body (31), the The main body (31) is slidably arranged in the pair of sliding grooves (232) of the upper cover (23), and the positions of the dropping holes (33) correspond to the reaction openings (231), and communicate with each other for the reaction Membrane (21). The biochip detection device according to claim 1, wherein the reaction module (20) further includes at least one filter membrane (24), which is arranged between the upper cover (23) and the sliding cover (30), and corresponds to The dropping holes (33) of the sliding cover (30) and the reaction opening (231) of the upper cover (23). The biochip detection device as described in claim 2, wherein the body (31) of the sliding cover (30) is provided with a first recess (311), and the filter membrane (24) is set and positioned in the first recess (311 )Inside. The biochip detection device as claimed in claim 1, wherein the hole wall of each drip hole (33) is conical. The biochip detection device according to claim 1, wherein the base (10) is provided with a second recess (11), and the reaction film (21) is arranged and positioned in the second recess (11). The biochip detection device as described in claim 1, wherein the body (31) has a drop-in area (F), and the drop-in holes (33) are located in the drop-in area (F), and the drop-in area (F) The central position of the area (F) is outwardly arranged in a spaced circle. The biochip detection device as claimed in item 1, wherein the aperture of the reaction opening (231) is larger than the aperture of the liquid absorption opening (221), and the liquid absorption area (222) is partially exposed in the reaction opening ( 231). The biochip detection device as described in claim 1, wherein the size of the reaction film (21) is smaller than the size of the diffusion film (22), and the reaction film (21) resists the suction hole (221) and the local suction liquid area (222). The biochip detection device as claimed in item 1, wherein the diffusion film (22) has two liquid-absorbing openings (221), and the size of the reaction opening (231) is larger than the two liquid-absorbing openings (221 ) is the sum of the dimensions. The biochip detection device according to claim 9, wherein the two sliding covers (30) are separately arranged in the pair of slide slots (232) slidably arranged in the upper cover (23). The biochip detection device as described in claim 9, wherein the dropping holes (33) of the sliding cover (30) are divided into two groups, and the dropping holes (33) of the two groups correspond to On the two suction holes (221). The biochip detection device as described in claim 1, wherein the diffusion film (22) includes a first half-diffusion film (223) and a second half-diffusion film (224), the first half-diffusion film (223) and The second half-diffusion film (224) is partially overlapped and connected to the opposite sides of the reaction film (21), and the first half-diffusion film (223) and the second half-diffusion film (224) are separated The liquid-absorbing opening (221) is formed at a predetermined distance. The biochip detection device as described in claim item 1, wherein each of the drip holes (33) is for a liquid to be tested (40) to drip into and penetrate through the reaction opening (231), the liquid suction opening ( 221) to the reaction film (21) to form a region to be measured, when the body (31) moves away from the reaction module (20) through the chutes (232), the reaction opening (231) and the The liquid-absorbing opening (221) is for a color-forming agent (50) to drip into the center around the area to be tested, and the color-forming agent (50) is adsorbed and diffused toward the surrounding through the liquid-absorbing area (222) to the area to be tested to form a reaction with each other and obtain the final test result. The biochip detection device according to claim 13, wherein the color forming agent (50) includes reactive antibodies and colloidal gold, and the volume of the test solution (40) is between 0.1 micrograms and 0.2 micrograms.

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