JPH04138364A - Plate for shaking examination - Google Patents
Plate for shaking examinationInfo
- Publication number
- JPH04138364A JPH04138364A JP26204590A JP26204590A JPH04138364A JP H04138364 A JPH04138364 A JP H04138364A JP 26204590 A JP26204590 A JP 26204590A JP 26204590 A JP26204590 A JP 26204590A JP H04138364 A JPH04138364 A JP H04138364A
- Authority
- JP
- Japan
- Prior art keywords
- liquid reservoir
- plate
- shaking
- side wall
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000012360 testing method Methods 0.000 claims description 26
- 230000004520 agglutination Effects 0.000 abstract description 18
- 238000006243 chemical reaction Methods 0.000 abstract description 17
- 238000000034 method Methods 0.000 abstract description 5
- 238000007689 inspection Methods 0.000 description 6
- 238000000465 moulding Methods 0.000 description 5
- 238000004220 aggregation Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009535 clinical urine test Methods 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- -1 polynystyl Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003856 thermoforming Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
【発明の詳細な説明】
〈産業上の利用分野〉
本発明は振盪検査用プレートに関し、詳しくは免疫学的
凝集反応検査において凝集反応の観察および判定が容易
となる臨床検査用プレートに関するものである。[Detailed Description of the Invention] <Industrial Application Field> The present invention relates to a shaking test plate, and more particularly to a clinical test plate that facilitates the observation and determination of agglutination reactions in immunological agglutination tests. .
〈従来の技術〉
一般に免疫学的凝集反応の検査は、免疫学的な反応を起
こす抗原または抗体の何れか一方、または両者を組み合
わせて使用し、これに体液などの被検液とを混合して抗
原抗体反応を行ない、凝集像の有無により抗体または抗
原の存在を確認している。<Prior art> In general, immunological agglutination tests use either antigens or antibodies that cause an immunological reaction, or a combination of both, and are mixed with test fluids such as body fluids. The presence of antibodies or antigens is confirmed by the presence or absence of aggregated images.
通常、このような凝集反応を利用した臨床検査では、被
検液と検査試薬とを混合、攪拌して反応させる際に、凹
状の液体溜めを設けてなる検査用プレートが用いられて
おり、この液体溜め内で凝集反応を行なっている。この
検査プレーＩ−は機械的強度の点からはプラスチック製
のプレートが好ましく、各種形状のプレートか提案され
ている。Normally, in clinical tests that utilize such agglutination reactions, a test plate with a concave liquid reservoir is used when the test liquid and test reagent are mixed, stirred, and reacted. A flocculation reaction is taking place within the liquid reservoir. The test plate I- is preferably made of plastic from the viewpoint of mechanical strength, and plates of various shapes have been proposed.
凝集反応によって生じる凝集像は肉眼または顕微鏡にて
観察するが、四部に投入し７だ被検液および検査試薬と
か均一に混合されないと、凝集反応が均一・に起こりが
たく、また判定しにくいという問題がある。従って、微
妙な凝集反応を観察する場合は液体の均一・な混合が特
に重要条件となるのである。The agglutination image produced by the agglutination reaction can be observed with the naked eye or with a microscope, but unless the test liquid and test reagent are mixed uniformly, the agglutination reaction will not occur uniformly and it will be difficult to judge. There's a problem. Therefore, uniform mixing of liquids is a particularly important condition when observing delicate aggregation reactions.
検査プレートに設けられている凹状の液体溜めの形状と
しては、実開昭５４−７４５９３号公報に開示されてい
るような半球状や半球状の底部か平坦なものが提案され
ている。半球状の液体溜めでは底部の中央が深くなって
いるので、凝集反応の際に凝集塊が中央部に偏り、凝集
像の観察を行ない難く、特に微妙な凝集反応を行なうに
は不利である。また底部か平坦な液体溜めでもよ液体か
均一に混ざりにくいだけてなく、液体および凝集塊が底
部の周縁に偏り、やはり凝集像の観察を行ないかたいも
のである。As the shape of the concave liquid reservoir provided in the inspection plate, a hemispherical shape, a hemispherical bottom shape, or a flat one as disclosed in Japanese Utility Model Application Publication No. 54-74593 has been proposed. In a hemispherical liquid reservoir, the center of the bottom is deep, so during the agglutination reaction, the aggregates are biased towards the center, making it difficult to observe the agglutination image, which is particularly disadvantageous for performing delicate agglutination reactions. Furthermore, even in a liquid reservoir with a flat bottom, it is not only difficult to mix the liquid uniformly, but also the liquid and aggregates are concentrated around the periphery of the bottom, making it difficult to observe the aggregate image.
また、上記のいずれの形状の場合も液体溜めの側壁に付
着した液か乾燥して生じる乾燥物が、検査プレー１−を
振盪中に再び液中に入ることにより、反応によって生じ
た凝集像と混在し、正確に凝集反応の判定を行ないにく
くなる。In addition, in any of the above shapes, if the liquid adhering to the side wall of the liquid reservoir or the dried matter that dries enters the liquid again while shaking the test plate 1-, it may cause an agglomerated image generated by the reaction. This makes it difficult to accurately determine the agglutination reaction.
〈発明か解決しようとする課題〉
従って、本発明の目的は一上記従来の検査プレーＩ・か
有する課題を解決し、液体溜め内の液体を均一に混合で
き、液体内に生じる凝集像か均一・に分散可能となり、
凝集像、特に微妙な凝集反応によって生じる凝集像の観
察および判定か容易に行なえる検査用プレー１−を提供
することにある。<Problem to be Solved by the Invention> Accordingly, an object of the present invention is to solve the above-mentioned problems of the conventional inspection plate, to be able to uniformly mix the liquid in the liquid reservoir, and to make it possible to uniformly mix the agglomerated image formed in the liquid.・It becomes possible to disperse into
It is an object of the present invention to provide an inspection plate 1- which allows easy observation and judgment of agglutinated images, especially agglutinated images generated by delicate agglutination reactions.
〈課題を解決するだめの手段〉
本発明は上記目的を達成するためになされたものてあっ
て、凹状の液体溜めの壁面形状を特定形状とすることに
よって、凝集像の観察および判定か極めて簡単となる検
査用プレートか提供できることを見い出し、本発明を完
成させるに至った。<Means for Solving the Problem> The present invention has been made to achieve the above-mentioned object, and by making the wall surface shape of the concave liquid reservoir into a specific shape, it is possible to observe and judge the agglomerated image extremely easily. The present inventors have discovered that it is possible to provide a test plate that satisfies the requirements of the present invention, and have completed the present invention.
即ち、本発明の振の検査用プレートは、凹状の液体溜め
を有する振盪検査用プレーＩにおいて、液体溜めが側壁
と下方に湾曲する底壁とから形成され、かつ両壁面の接
する部分に角かｌＩ：いことを特徴とするものである。That is, the shaking testing plate of the present invention is a shaking testing plate I having a concave liquid reservoir, in which the liquid reservoir is formed of a side wall and a bottom wall that curves downward, and a corner is formed in the part where both wall surfaces contact. lI: It is characterized by being ugly.
以下、本発明の振の検査用プレーＩ・を図面により具体
的に説明する。Hereinafter, the swing testing play I of the present invention will be explained in detail with reference to the drawings.
第１図は本発明の振盪検査用プレートに設りる凹状の液
体溜めの平面図であり、第２図は第１図の液体溜めをＡ
−Ａ ’線上で切断した際の断面図である。FIG. 1 is a plan view of a concave liquid reservoir provided in the shaking test plate of the present invention, and FIG. 2 is a plan view of the liquid reservoir in FIG.
- It is a sectional view when cut on the A' line.
液体溜めｌは振盪検査用プレー１〜の平面に対して凹状
に形成されており、側壁２および底壁３からなるもので
ある。側壁２は第２図にて示されるように、傾斜面とす
ることか好ましい。側壁２か垂直向であると検査用プレ
ー１〜を成形する場合、成形型から成形後の検査用プレ
ー１を外しがたく、また凝集像の観察、判定性に影響を
及はし、正確な判定か行なえないことがある。従って、
好ましくは垂下方向に対して側壁２の角度を約１５〜４
５度の範囲とする。The liquid reservoir 1 is formed in a concave shape with respect to the plane of the shaking test plate 1 and consists of a side wall 2 and a bottom wall 3. It is preferable that the side wall 2 has an inclined surface as shown in FIG. If the side wall 2 is vertically oriented, when molding the inspection plates 1 to 1, it will be difficult to remove the inspection plates 1 after molding from the mold, and this will affect the observation and determination of the agglomerated image, making it difficult to accurately Sometimes it is not possible to make a judgment. Therefore,
Preferably, the angle of the side wall 2 with respect to the hanging direction is about 15 to 4
The range is 5 degrees.
本発明における底壁３ば平坦状てばなく、下方にやや湾
曲していることが必要である。均一・に液が混合し、ま
た底部中央に凝集塊が偏らないためには、液体溜め１の
開ロ直径力月Ｏ〜５０ｎ＋＋ｎの場合に湾曲の程度を１
０〜・８０Ｒ程度にすることが好ましい。In the present invention, it is necessary that the bottom wall 3 is not flat but slightly curved downward. In order to mix the liquid uniformly and to prevent aggregates from being concentrated in the center of the bottom, when the opening diameter of the liquid reservoir 1 is 0 to 50n++n, the degree of curvature must be set to 1.
It is preferable to set it to about 0 to 80R.
本発明においては上記側壁２および底壁３の接触部に角
がないごとが重要である。接触部に角があると液か均一
に混合されないだけてなく、凝集塊か底壁の周縁に偏り
、微妙な凝集反応によって生ずる凝集像の観察を行ない
にくくなる。従って、該接触部は０．３〜２Ｒ程度とす
ることが好ましい。In the present invention, it is important that the contact portion between the side wall 2 and the bottom wall 3 has no corners. If there are corners in the contact area, not only will the liquid not be mixed uniformly, but the agglomerates will be concentrated near the periphery of the bottom wall, making it difficult to observe the agglomerate image produced by the delicate aggregation reaction. Therefore, it is preferable that the contact portion has a radius of approximately 0.3 to 2R.
本発明の振盪検査用プレートを形成するための材料ば、
特に限定されないが、機械的強度および生産性の点から
はプラスチック、例えばポリニスチル、ポリスチレン、
ポリカーホ不−１・などを用いることが好ましく、射出
成形、Ｊｌｌｌ熱成形などの公知の成形方法によって成
形する。Materials for forming the shaking test plate of the present invention:
Although not particularly limited, from the viewpoint of mechanical strength and productivity, plastics such as polynystyl, polystyrene,
It is preferable to use polycarbonate, and molding is performed by a known molding method such as injection molding or Jllll thermoforming.
凹状の液体溜め１はその内面（壁面）を酸・アルカリ処
理法、プラスマ照射処理法、界面活性剤添加法、水不溶
性親水性高分子塗布法などの親水化処理を施すことが好
ましく、液体と液体溜め１との接触部の界面張力か減少
し、凝集像を観察する際に効果的である。The inner surface (wall surface) of the concave liquid reservoir 1 is preferably subjected to a hydrophilic treatment such as an acid/alkali treatment method, a plasma irradiation treatment method, a surfactant addition method, or a water-insoluble hydrophilic polymer coating method. The interfacial tension at the contact portion with the liquid reservoir 1 is reduced, which is effective in observing aggregate images.
本発明の振盪検査用プレートに設ける液体溜め１は、プ
レー！・成形時に少なくとも一個形成されるが、複数の
被検体を同時に検査するためには第３図に示すように、
複数個形成することが好ましい。また、液体溜め１の形
状は第２図に示すように、プレート上面から凹状とした
り、振盪検査用プレートを振盪した際に液体溜め１から
液体が溢れないようにするために、第４図に示すように
液体溜め１を取り囲んだ突出部４をプレート面上に形成
することもできる。The liquid reservoir 1 provided in the shaking test plate of the present invention is a PLAY!・At least one is formed during molding, but in order to test multiple objects at the same time, as shown in Figure 3,
It is preferable to form a plurality of them. In addition, the shape of the liquid reservoir 1 is concave from the top surface of the plate as shown in Figure 2, and the shape of the liquid reservoir 1 is as shown in Figure 4 to prevent the liquid from overflowing from the liquid reservoir 1 when the shaking test plate is shaken. As shown, a protrusion 4 surrounding the liquid reservoir 1 can also be formed on the plate surface.
なお、本発明の振盪検査用プレートは、凝集反応待に振
盪操作か必要であるので、手で持ちやすくするためにプ
レート周囲にリブを設けることが好ましい。Since the shaking test plate of the present invention requires a shaking operation during the agglutination reaction, it is preferable to provide ribs around the plate to make it easier to hold by hand.
〈発明の効果〉
本発明の１）客り尿検査用プレー１〜は以」二ように、
特定の形状を有する凹状の液体溜めを有しているので、
凝集反応によって生じる凝集像の観察、判定が容易であ
り、特に微妙な凝集反応において従来のものと比へて精
度良く判定できるという効果を奏する。また、上記形状
の液体溜めであるので、液か均一に混合でき、しかも振
の時に液が側壁に件名して生しる乾燥物の生成量が少な
いので、判定精度が向上する。<Effects of the Invention> 1) Customer urine test play 1 to 1 of the present invention are as follows:
Since it has a concave liquid reservoir with a specific shape,
It is easy to observe and judge the agglutination image produced by the agglutination reaction, and it has the effect of being able to judge particularly delicate agglutination reactions with higher precision than conventional methods. Further, since the liquid reservoir has the above-mentioned shape, the liquid can be mixed uniformly, and the amount of dry matter produced by the liquid on the side wall during shaking is small, so the determination accuracy is improved.
第１図は本発明の振盪検査用プレートに設ける液体溜め
の平面図、第２図は第１図の液体溜めをＡ Ａ’線ト
にて切断した断面図、第３図は本発明の振の検査用プレ
ートを一実例を示す平面図、第４図は他の実例の液体溜
め形状の断面図である。FIG. 1 is a plan view of a liquid reservoir provided in a shaking test plate of the present invention, FIG. 2 is a cross-sectional view of the liquid reservoir of FIG. FIG. 4 is a plan view showing one example of the inspection plate, and FIG. 4 is a sectional view of another example of the shape of a liquid reservoir.
Claims (1)
いて、液体溜めが側壁と下方に湾曲する底壁とから形成
され、かつ両壁面の接する部分に角がないことを特徴と
する振盪検査用プレート。(1) A shaking test plate having a concave liquid reservoir, characterized in that the liquid reservoir is formed from a side wall and a bottom wall that curves downward, and there is no corner at the part where both wall surfaces touch. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26204590A JP2862360B2 (en) | 1990-09-29 | 1990-09-29 | Shaking test plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26204590A JP2862360B2 (en) | 1990-09-29 | 1990-09-29 | Shaking test plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04138364A true JPH04138364A (en) | 1992-05-12 |
| JP2862360B2 JP2862360B2 (en) | 1999-03-03 |
Family
ID=17370266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP26204590A Expired - Fee Related JP2862360B2 (en) | 1990-09-29 | 1990-09-29 | Shaking test plate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2862360B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006133168A (en) * | 2004-11-09 | 2006-05-25 | Murazumi Kogyo Kk | Sample inspecting plate |
-
1990
- 1990-09-29 JP JP26204590A patent/JP2862360B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006133168A (en) * | 2004-11-09 | 2006-05-25 | Murazumi Kogyo Kk | Sample inspecting plate |
| JP4494167B2 (en) * | 2004-11-09 | 2010-06-30 | 村角工業株式会社 | Sample inspection plate |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2862360B2 (en) | 1999-03-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |