JP2813449B2 - Laboratory test plate - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a clinical test plate, and more particularly, to a clinical test plate that facilitates observation and determination of agglutination in an immunological agglutination test. .

<Prior art> In general, an immunological agglutination test is performed by using one or both of an antigen and an antibody that cause an immunological reaction, and mixing with a test solution such as a body fluid. An antigen-antibody reaction is performed to confirm the presence of the antibody or antigen by the presence or absence of the aggregated image. In such an agglutination reaction, various test reagents have been developed for fixing an antigen or antibody to a particulate carrier such as a latex to facilitate confirmation at the time of agglutination.

In clinical tests using such agglutination,
When the test solution and the test reagent are mixed, stirred and reacted,
A clinical test plate provided with a concave liquid reservoir is used, and an agglutination reaction is performed in the liquid reservoir.

Here, when the agglutination reaction is observed on the clinical test plate, it is important for a delicate judgment whether or not a slight agglutination reaction can be easily observed. When using a conventional opaque plate made of paper, hold the plate with both hands and shake it back and forth, right and left, observe the face close under the fluorescent light, and also use a transparent plate such as a plastic sheet. When used, an inspection plate is placed on an apparatus that is illuminated with a fluorescent lamp from underneath, such as an immune observation box, to observe.

Further, Japanese Utility Model Application Laid-Open No. 57-201950 discloses an inspection plate comprising a transparent plate as an upper molded product having a concave well and an opaque lower molded product which can be combined with and separated from the transparent plate. This plate facilitates observation with the naked eye and with a microscope by combining and separating the upper molded product and the lower molded product.

<Problems to be Solved by the Invention> As a clinical test plate, a plastic plate is preferable from the viewpoint of strength. Furthermore, Akira Kai
In the case of the plate disclosed in Japanese Patent Application Laid-Open No. 57-201950, observation with both the naked eye and a microscope is possible, and it is easy to determine the agglutination reaction.

However, the plate devised in the above publication has a flat bottom surface of the well, and is difficult to separate easily because the bottom surface of the well is in close contact with the lower molded product. Further, when the bottom surface of the well is flat, when a delicate agglutination reaction occurs, the flow of the liquid in the well may not be smooth, and it may be difficult to observe the agglutination image.

<Means for Solving the Problems> The present invention has been made in order to solve the problems of the above-mentioned conventional clinical test plate, and is intended for a clinical test in which a fine aggregate image can be easily observed with the naked eye and a microscope. It is intended to provide a plate.

That is, the clinical test plate of the present invention comprises a transparent plastic test plate in which a concave liquid reservoir having a curved bottom surface is formed, and an opaque plate removably fitted to a lower portion of the plate. The part is fitted without contacting the opaque plate.

 Hereinafter, the present invention will be specifically described with reference to the drawings.

FIG. 1 shows a transparent plastic inspection plate 1 before fitting, and an opaque plate 2 removably fitted below the plate.

The inspection plate 1 can be manufactured by heat molding a transparent plastic material, for example, polyester, polystyrene, polycarbonate, or the like. At least one concave liquid reservoir (well) 3 provided on the inspection plate 1 and having a curved bottom surface is formed when the plate is formed. In order to simultaneously inspect a plurality of subjects, it is preferable to form a plurality of subjects. When the inner surface of the liquid reservoir 3 is subjected to a hydrophilization treatment by an acid / alkali treatment method, a plasma irradiation treatment method, a surfactant addition method, a water-insoluble hydrophilic polymer coating method, or the like, the interface between the liquid and the liquid reservoir is contacted. The tension is reduced, which is effective when observing an aggregated image.

A fitting portion 4 used for fitting with the opaque plate 2 located below is formed at the edge of the inspection plate, and can be fitted with a fitting portion 4 ′ formed on the opaque plate 2. As shown in FIG. 2, the liquid reservoir 3 is fitted so that the bottom surface of the liquid reservoir 3 does not contact the sheet 2. The reason is unknown, but by fitting the bottom surface of the liquid reservoir 3 and the sheet 2 so as not to come into contact, a coagulated image can be clearly observed,
The judgment is also easy.

The opaque plate 2 is formed of a plastic material or a paper material into a shape that can be fitted to the inspection plate 1 at the edge. The opaque plate 2 is made of an opaque material,
It can be obtained by molding from a material containing a colorant such as black or white, or by producing a transparent material and then applying a colorant such as black or white.

3A to 3C show combinations of the inspection plate 1 and the opaque plate 2 in various shapes.

FIGS. 3 (a) and 3 (c) show an example in which the fitting portion 4 of the inspection plate 1 and the fitting portion 4 'of the opaque plate 2 are fitted, and FIG. An example in which the inspection plate 1 and the opaque plate 2 are fitted so as to be inserted into the folded portion is shown.

<Effect of the Invention> Since the clinical test plate of the present invention has the above-described configuration, when an opaque sheet is fitted to the test plate to cause an agglutination reaction, observation of the agglutination image with the naked eye,
The judgment is easy. When the opaque sheet is removed from the test plate and subjected to an agglutination reaction, the test plate is made of transparent plastic, so that measurement using a microscope can be performed. Since the bottom surface of the liquid reservoir of the test plate and the opaque sheet are not in close contact with each other, the operation of removing the opaque sheet becomes easy, and the handleability is excellent.
Further, when the inspection plate and the opaque sheet are fitted to each other to observe the aggregation image with the naked eye, the aggregation image can be clearly observed.

[Brief description of the drawings]

FIG. 1 is a cross-sectional view of a transparent plastic test plate before fitting and an opaque plate removably fitted to a lower portion thereof, which constitutes the clinical test plate of the present invention, and FIG. Sectional view of the combined state, FIG.
(C) is a sectional view of a combination of the inspection plate and the opaque plate in various shapes. 1 ... transparent plastic inspection plate 2 ... opaque plate 3 ... liquid reservoir 4,4 '... fitting part

──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int.Cl. ⁶ , DB name) G01N 33/543

Claims (1)

(57) [Claims] 1. A transparent plastic inspection plate having a concave liquid reservoir having a curved bottom surface, and an opaque plate removably fitted to a lower portion of the plate, wherein the liquid reservoir is in contact with the opaque plate. A clinical test plate that is fitted without fitting.