JP4373025B2 - Egg cryopreservation tool and cylindrical member holding device - Google Patents

Egg cryopreservation tool and cylindrical member holding device Download PDF

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JP4373025B2
JP4373025B2 JP2001120225A JP2001120225A JP4373025B2 JP 4373025 B2 JP4373025 B2 JP 4373025B2 JP 2001120225 A JP2001120225 A JP 2001120225A JP 2001120225 A JP2001120225 A JP 2001120225A JP 4373025 B2 JP4373025 B2 JP 4373025B2
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cylindrical member
egg
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JP2002315573A (en
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太 井上
保 井上
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株式会社北里サプライ
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
    • A61D19/024—Tube-like containers, e.g. straws

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  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

【０００１】
【発明の属する技術分野】
本発明は、哺乳動物の卵子、胚などの卵を凍結保存する際に使用する卵凍結保存用具に関する。
【０００２】
【従来の技術】
哺乳動物胚の凍結保存は、特定の系統や品種の遺伝資源の保存を可能とする。また、絶滅の危機に瀕している動物種の維持にも有効である。さらに、ヒトの不妊治療においても有用である。
【０００３】
哺乳動物胚の凍結保存方法としては、特開２０００−１８９１５５公報において、哺乳動物胚または卵子を滅菌処理した凍結ストロー、凍結バイアルまたは凍結チューブ等の凍結保存用容器の内面に、これらの胚または卵子を包被するに充分な最少量のガラス化液で貼り付け、この凍結保存用容器を密封し、そしてこの容器を液体窒素に接触させて急速に冷却することが提案されている。そして、融解方法では、前記の方法で保存した凍結保存用容器を前記液体窒素から取り出し、容器の一端部を開口し、この容器内に３３〜３９℃の希釈液を直接注入し、胚または卵子の凍結を融解希釈するものである。この方法によれば、哺乳動物胚または卵子をウイルスや細菌による感染のおそれがなく高い生存率で保存および融解希釈することのできるという優れた効果を備えている。
【０００４】
【発明が解決しようとする課題】
しかし、凍結ストロー、凍結バイアルまたは凍結チューブ等の凍結保存用容器の内面に、胚または卵子などの卵を包被するに充分な最少量のガラス化液で貼り付ける作業が必ずしも容易ではなかった。
そこで、本発明の目的は、卵の凍結保存作業を確実かつ容易に行うことができる卵凍結保存用具を提供するものである。
【０００５】
【課題を解決するための手段】
上記の目的を達成するものは、液体窒素耐性材料により形成された本体部と、該本体部の一端に取り付けられ、可撓性かつ透明性かつ液体窒素耐性材料により形成された卵付着保持用ストリップと、該卵付着保持用ストリップを被包可能に前記本体部に着脱自在に取り付けられる一端が封鎖され、かつ液体窒素耐性材料により形成された筒状部材とからなる卵凍結保存用具である。
そして、前記本体部は、細長い平板状本体部であることが好ましい。また、前記卵付着保持用ストリップは、細長い平坦フィルム状の卵付着保持用ストリップであることが好ましい。また、前記筒状部材は、一端側内部に挿入された綿状体により通気可能に封止されていることが好ましい。さらに、前記本体部は、前記卵付着保持用ストリップの一端を保持するとともに前記筒状部材の他端内に侵入し係合する円柱状部を備えていることが好ましい。また、前記筒状部材は、他端部がテーパー状に拡径するテーパー部となっていることが好ましい。
【０００６】
また、上記目的を達成するものは、先端側に挟持部を備え、後端側に把持部を備える２つの保持部材からなり、該２つの保持部材の前記挟持部と前記把持部間において回動自在に軸支された鋏鉗子状の筒状部材保持器具であって、前記２つの保持部材の向かい合う挟持部には、細径の筒状部材を実質的に潰すことなく保持するために向かい合う一組の凹部が形成されている筒状部材保持器具である。
そして、前記筒状部材保持器具は、上記の卵凍結保存用具の前記筒状部材を保持するためのものである。そして、前記筒状部材保持器具は、前記筒状部材を保持した状態を維持するための２つの保持部材に設けられた保持状態維持機構を備えていることが好ましい。
【０００７】
【発明の実施の形態】
本発明の卵凍結保存用具を図面に示す実施例を用いて説明する。
図１は、本発明の実施例の卵凍結保存用具の正面図である。
図２は、図１に示した卵凍結用保存用具の筒状部材を本体部材より離脱させた状態の正面図である。図３は、図２に示した卵凍結用保存用具の本体部材の側面図である。図４は、図１に示した卵凍結保存用具の本体部材の先端部分および筒状部材の拡大正面図である。図５は、図１に示した卵凍結保存用具の本体部材の先端部分および筒状部材の拡大断面図である。
【０００８】
本発明の卵凍結保存用具１は、耐寒性材料により形成された本体部２と、本体部２の一端に取り付けられ、可撓性かつ透明性かつ液体窒素耐性材料により形成された卵付着保持用ストリップ３と、卵付着保持用ストリップ３を被包可能に本体部２に着脱自在に取り付けられる一端が封鎖され、かつ耐寒性材料により形成された筒状部材４とからなるものである。
本発明の卵凍結保存用具は、卵ガラス化保存用具、卵子または胚凍結保存用具、卵子または胚ガラス化保存用具と言い換えることができる。
本発明の卵凍結保存用具は、卵凍結保存用具本体部材１０と、これに着脱自在に取り付けられる筒状部材４とからなる。なお、筒状部材４を設けず、卵凍結保存用具本体部材１０を単独としても卵凍結保存用具として使用することもできる。
卵凍結保存用具本体部材１０は、耐寒性材料により形成された本体部２と、本体部２の一端に取り付けられ、可撓性かつ透明性かつ液体窒素耐性材料により形成された卵付着保持用ストリップ３とからなる。
【０００９】
この実施例の卵凍結保存用具本体部材１０では、本体部２は、細長い平板状本体部となっている。本体部の長さとしては、５０〜２００ｍｍ程度が好適である。また、平板状本体部の場合の幅としては、３〜１０ｍｍ程度が好適であり、厚さは、１〜３ｍｍ程度が好適である。そして、本体部材１０は、卵付着保持用ストリップ３の一端を保持するとともに筒状部材４の他端内に侵入し係合する円柱状部５を備えている。この円柱状部は、先端に向かってテーパー状に若干縮径している。円柱状部の外径としては、０．５〜３ｍｍ程度が好適である。そして、円柱状部５の先端には、図５に示すように、卵付着保持用ストリップ３の一端を収納するための軸方向に延びる凹部が形成されている。
本体部２は、ポリエステル（例えば、ポリエチレンテレフタレート、ポリブチレンテレフタレート）、ポリオレフィン（例えば、ポリエチレン、超高分子量ポリエチレン、ポリプロピレン、エチレン−プロピレン共重合体、エチレン−酢酸ビニル共重合体）、スチレン系樹脂（例えば、ポリスチレン、メタクリレート−スチレン共重合体、メタクリレート−ブチレン−スチレン共重合体）、ポリアミド（例えば、６ナイロン，６６ナイロン）、ポリサルホン、フッ素樹脂、ポリイミド等の耐寒性樹脂が使用され、特に、液体窒素の温度に耐え得るものが好ましい。
【００１０】
卵付着保持用ストリップ３は、可撓性かつ透明性かつ液体窒素耐性材料により形成されており、この実施例では、細長い平坦フィルム状のものとなっている。ストリップ３は、卵付着作業を容易にするために、可撓性かつ無色透明な平坦フィルム状のものが用いられている。卵付着保持用ストリップ３の露出する部分の長さは、１０〜３０ｍｍ程度、幅０．５〜２．０ｍｍ程度、厚さが０．０１〜０．４ｍｍ程度のものが用いられる。
そして、卵付着保持用ストリップ３は、他端部（基端部）が、上述した本体部２の凹部に収納され、接着剤により本体部２に固着されている。
卵付着保持用ストリップ３の形成材料としては、例えば、３−フッ化ポリエチレン、低密度ポリエチレン、中密度ポリエチレン、高密度ポリエチレン、ポリカーボネート、ナイロン、ポリスルホン、ポリエステル、ポリスチレン、ポリイミド、超高分子量ポリエチレン、エチレン−酢酸ビニル共重合体などの合成樹脂からなるフィルムあるいはそれらの積層体が挙げられる。特に、液体窒素耐性材料、言い換えれば、液体窒素に接触しても脆化しないものが使用される。
【００１１】
筒状部材４は、卵付着保持用ストリップ３を被包可能に本体部２に着脱自在に取り付けられる一端が封鎖され、かつ耐寒性材料により形成されたものである。特に、この実施例の筒状部材４は、一端側内部に挿入された綿状体１２により通気可能に封止されており、他端部がテーパー状に拡径するテーパー部となっている。そして、筒状部材４内に収納された綿状体１２は接着剤１３により固定されている。
筒状部材４は、卵付着保持用ストリップ３に接触することなく被包できる内径および長さを備えている。そして、筒状部材４がテーパー部を備えることにより、ストリップ３の筒状部材内への挿入を容易にしているとともに、本体部２の円柱状部分の筒状部材内への侵入も容易なものとしている。
筒状部材４の形成材料としては、例えば、３−フッ化ポリエチレン、低密度ポリエチレン、中密度ポリエチレン、高密度ポリエチレン、ポリカーボネート、ナイロン、ポリスルホン、ポリエステル、ポリスチレン、ポリイミド、超高分子量ポリエチレン、エチレン−酢酸ビニル共重合体などの合成樹脂からなるフィルムあるいはそれらの積層体が挙げられる。特に、液体窒素耐性材料、言い換えれば、液体窒素に接触しても脆化しないものが好ましい。
【００１２】
次に、本発明の筒状部材保持器具について図面に示した実施例を用いて説明する。
図６は、本発明の筒状部材保持器具の正面図である。図７は、図６に示した筒状部材保持器具の解放状態の先端部分拡大図である。
本発明の筒状部材保持器具２０は、先端側に挟持部２５，２６を備え、後端側に把持部２３，２４を備える２つの保持部材２１，２２からなり、２つの保持部材２１，２２の挟持部２５，２６と把持部２３，２４間において回動自在に軸支された鋏鉗子状の筒状部材保持器具である。そして、２つの保持部材２１，２２の向かい合う挟持部２５，２６には、細径の筒状部材を実質的に潰すことなく保持するための向かい合う一組の凹部３１，３２が形成されている。
この筒状部材保持器具は、上述した卵凍結保存用具の筒状部材４を保持するために有効に使用される。
【００１３】
本発明の筒状部材保持器具２０は、２つの保持部材２１，２２からなり、それらは、ほぼ左右対称なものとなっている。保持部材２１は、先端側に挟持部２５を後端側に把持部２３を備えている。同様に、保持部材２２は、先端側に挟持部２６を後端側に把持部２４を備えている。挟持部２５，２６には、向かい合う刃部２７，２８と、挟持部２５，２６の先端部に設けられた向かい合う一対の凹部３１，３２を備えている。また、２つの保持部材２１，２２は、挟持部２５，２６と把持部２３，２４間において、軸２９により回動自在に軸支されている。そして、凹部３１，３２は、筒状部材保持器具２０の上記凹部３１，３２部分において、筒状部材４を挟持したとき、細径の筒状部材を実質的に潰すことなく保持できる大きさに形成されている。具体的には、凹部３１，３２により構成される円状開口部の内径が保持する筒状部材の外径より若干小さくなるように形成されている。
【００１４】
さらに、筒状部材保持器具は、図６および図８に示すように、筒状部材を保持した状態を維持するための２つの保持部材２１，２２に設けられた保持状態維持機構３５，３６を備えている。具体的には、２つの保持部材２１，２２の把持部２３，２４付近より他方の保持部材方向に延びる平板状突起部３５，３６を備えており、この平板状突起部の接触する内面には、図８に示すように、断面鋸刃状の段階的係合可能部が形成されている。このため、筒状部材保持器具２０の凹部部分において筒状部材を挟持した状態において、保持部材２１，２２の把持部２３，２４を近接する方向に押すと、保持状態維持機構３５，３６の断面鋸刃状部分が係合し、その状態を維持するようになっている。このため、この筒状部材保持器具２０では、筒状部材を保持状態の維持に特別な力を加えている必要がなく、筒状部材４をストリップ３を被包し、かつ、本体部２に取り付ける操作が容易である。また、保持状態維持機構３５，３６の係合は、横方向、言い換えれば、図８の上下方向に、２つの保持部材２１，２２を広げることにより解除することができる。
筒状部材保持器具は、ステンレス鋼、チタン合金などの金属により作製される。
【００１５】
次に、本発明の卵凍結保存用具１および筒状部材保持器具２０の使用方法について説明する。
この説明では、卵子を凍結保存する場合を例に取り説明する。
まず、ピペットの先端に卵子を採取し、卵子の細胞内液を平衡液に置換する作業を行い、さらに、細胞外液をガラス化液に置換する作業を行う。そして、顕微鏡下において、本発明の卵凍結保存用具１の卵付着保持用ストリップ３の先端部に卵子を少量のガラス化液とともに付着させ、卵子が付着したストリップ部分をあらかじめ準備しておいた液体窒素に浸漬し凍結（ガラス化）させる。そして、あらかじめ準備してある筒状部材４を保持しその状態が維持されている筒状部材保持器具を用いて、筒状部材４を本体部材１０に取り付け、筒状部材保持器具を取り外した後、収納容器（ケーン）内に、ガラス化した卵子を付着保持する卵凍結保存用具を収納した後、収納容器を液体窒素タンク内に入れ保存する。
【００１６】
【発明の効果】
本発明の卵凍結保存用具は、耐寒性材料により形成された本体部と、該本体部の一端に取り付けられ、可撓性かつ透明性かつ液体窒素耐性材料により形成された卵付着保持用ストリップと、該卵付着保持用ストリップを被包可能に前記本体部に着脱自在に取り付けられる一端が封鎖され、かつ耐寒性材料により形成された筒状部材とからなるものである。
卵付着保持用ストリップを備えることにより、卵子もしくは胚などの卵をガラス化液と共に付着保持することが容易であり、さらに、卵の液体窒素への浸漬作業も容易に行うことができる。
【図面の簡単な説明】
【図１】図１は、本発明の実施例の卵凍結保存用具の正面図である。
【図２】図２は、図１に示した卵凍結用保存用具の筒状部材を本体部材より離脱させた状態の正面図である。
【図３】図３は、図２に示した卵凍結用保存用具の本体部材の側面図である。
【図４】図４は、図１に示した卵凍結保存用具の本体部材の先端部分および筒状部材の拡大正面図である。
【図５】図５は、図１に示した卵凍結保存用具の本体部材の先端部分および筒状部材の拡大断面図である。
【図６】図６は、本発明の筒状部材保持器具の正面図である。
【図７】図７は、図６に示した筒状部材保持器具の解放状態の先端部分拡大図である。
【図８】図８は、本発明の筒状部材保持器具の保持状態維持機構を説明するための説明図である。
【符号の説明】
１ 卵凍結保存用具
２ 本体部
３ 卵付着保持用ストリップ
４ 筒状部材[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an egg cryopreservation device for use in cryopreserving eggs such as mammalian eggs and embryos.
[0002]
[Prior art]
The cryopreservation of mammalian embryos allows the preservation of genetic resources of specific strains and varieties. It is also effective in maintaining endangered animal species. Furthermore, it is useful in the treatment of human infertility.
[0003]
As a method for cryopreserving mammalian embryos, in JP-A 2000-189155, these embryos or eggs are placed on the inner surface of a cryopreservation container such as a frozen straw, a frozen vial or a freezing tube in which mammalian embryos or eggs are sterilized. It has been proposed to paste with a minimum amount of vitrification solution sufficient to encapsulate, seal the cryopreservation container and allow the container to contact liquid nitrogen and cool rapidly. In the thawing method, the cryopreservation container stored by the above method is taken out from the liquid nitrogen, one end of the container is opened, and a diluted solution of 33 to 39 ° C. is directly injected into the container, and an embryo or egg Thaw and dilute the freeze. This method has an excellent effect that mammalian embryos or ova can be stored and melt-diluted with a high survival rate without the risk of infection by viruses or bacteria.
[0004]
[Problems to be solved by the invention]
However, it is not always easy to attach a vitrification solution with a minimum amount sufficient to encapsulate an egg such as an embryo or egg onto the inner surface of a cryopreservation container such as a freezing straw, a freezing vial or a freezing tube.
Therefore, an object of the present invention is to provide an egg cryopreservation tool that can reliably and easily perform an egg cryopreservation operation.
[0005]
[Means for Solving the Problems]
What achieves the above object is a main body portion formed of a liquid nitrogen resistant material, and a strip for attaching and holding an egg attached to one end of the main body portion and formed of a flexible, transparent and liquid nitrogen resistant material. And an egg cryopreservation tool comprising a cylindrical member formed of a liquid nitrogen resistant material and sealed at one end, which is detachably attached to the main body so as to encapsulate the egg adhesion holding strip.
And it is preferable that the said main-body part is an elongate flat plate-shaped main-body part. Moreover, it is preferable that the said egg adhesion holding strip is an elongated flat film-like egg adhesion holding strip. Moreover, it is preferable that the said cylindrical member is sealed by the cotton-like body inserted in the one end side inside so that ventilation | gas_flowing is possible. Furthermore, it is preferable that the main body portion includes a columnar portion that holds one end of the egg adhesion holding strip and enters and engages with the other end of the cylindrical member. Moreover, it is preferable that the said cylindrical member is a taper part which an other end part expands in a taper shape.
[0006]
In addition, what achieves the above-mentioned object is composed of two holding members each having a holding part on the front end side and a holding part on the rear end side, and is rotated between the holding part and the holding part of the two holding members. A cylindrical forceps-like cylindrical member holding device that is pivotally supported, wherein the two holding members face each other in order to hold the thin cylindrical member without being substantially crushed. It is a cylindrical member holding device in which a set of concave portions is formed.
And the said cylindrical member holding instrument is for hold | maintaining the said cylindrical member of said egg cryopreservation tool. And it is preferable that the said cylindrical member holding fixture is provided with the holding state maintenance mechanism provided in the two holding members for maintaining the state which hold | maintained the said cylindrical member.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
The egg cryopreservation device of the present invention will be described with reference to the embodiments shown in the drawings.
FIG. 1 is a front view of an egg cryopreservation tool according to an embodiment of the present invention.
FIG. 2 is a front view of the egg freezing preservation tool shown in FIG. 1 in a state where the cylindrical member is detached from the main body member. FIG. 3 is a side view of the main body member of the egg freezing preservation tool shown in FIG. 2. FIG. 4 is an enlarged front view of the distal end portion of the main body member and the cylindrical member of the egg cryopreservation tool shown in FIG. FIG. 5 is an enlarged cross-sectional view of the distal end portion of the main body member and the cylindrical member of the egg cryopreservation tool shown in FIG.
[0008]
The egg cryopreservation tool 1 according to the present invention is a main body 2 formed of a cold resistant material, and attached to one end of the main body 2, and is for attaching and holding an egg formed of a flexible, transparent and liquid nitrogen resistant material. The strip 3 and the tubular member 4 formed of a cold-resistant material are sealed off at one end, which is detachably attached to the main body 2 so that the egg attachment holding strip 3 can be encapsulated.
The egg cryopreservation device of the present invention can be restated as an egg vitrification storage device, an egg or embryo cryopreservation device, an egg or embryo vitrification storage device.
The egg cryopreservation tool of the present invention comprises an egg cryopreservation tool body member 10 and a cylindrical member 4 that is detachably attached to the egg cryopreservation tool body member 10. In addition, the cylindrical member 4 is not provided, and the egg cryopreservation tool body member 10 can be used alone or as an egg cryopreservation tool.
The egg cryopreservation tool main body member 10 includes a main body portion 2 formed of a cold-resistant material, and a strip for attaching and holding an egg attached to one end of the main body portion 2 and formed of a flexible, transparent, liquid nitrogen-resistant material. It consists of three.
[0009]
In the egg cryopreservation tool main body member 10 of this embodiment, the main body 2 is an elongated flat plate-shaped main body. The length of the main body is preferably about 50 to 200 mm. Moreover, as a width | variety in the case of a plate-shaped main-body part, about 3-10 mm is suitable, and about 1-3 mm is suitable for thickness. The main body member 10 includes a columnar portion 5 that holds one end of the egg attachment holding strip 3 and enters and engages the other end of the cylindrical member 4. The cylindrical portion is slightly reduced in diameter in a tapered shape toward the tip. The outer diameter of the columnar part is preferably about 0.5 to 3 mm. And the recessed part extended in the axial direction for accommodating the end of the strip 3 for egg adhesion holding | maintenance is formed in the front-end | tip of the cylindrical part 5 as shown in FIG.
The main body 2 is made of polyester (for example, polyethylene terephthalate, polybutylene terephthalate), polyolefin (for example, polyethylene, ultrahigh molecular weight polyethylene, polypropylene, ethylene-propylene copolymer, ethylene-vinyl acetate copolymer), styrene resin ( For example, cold-resistant resins such as polystyrene, methacrylate-styrene copolymer, methacrylate-butylene-styrene copolymer), polyamide (for example, 6 nylon, 66 nylon), polysulfone, fluororesin, polyimide, etc. are used. Those capable of withstanding the temperature of nitrogen are preferred.
[0010]
The egg adhesion holding strip 3 is formed of a flexible, transparent and liquid nitrogen resistant material. In this embodiment, the strip 3 is in the form of an elongated flat film. The strip 3 is a flat film that is flexible and colorless and transparent in order to facilitate the egg attachment work. The length of the exposed portion of the egg attachment holding strip 3 is about 10 to 30 mm, the width is about 0.5 to 2.0 mm, and the thickness is about 0.01 to 0.4 mm.
And the other end part (base end part) of the strip 3 for egg adhesion holding | maintenance is accommodated in the recessed part of the main-body part 2 mentioned above, and it adheres to the main-body part 2 with the adhesive agent.
Examples of the material for forming the egg attachment holding strip 3 include 3-fluorinated polyethylene, low density polyethylene, medium density polyethylene, high density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultrahigh molecular weight polyethylene, and ethylene. -A film made of a synthetic resin such as vinyl acetate copolymer or a laminate thereof. In particular, a liquid nitrogen resistant material, in other words, a material that does not become brittle when in contact with liquid nitrogen is used.
[0011]
The cylindrical member 4 is formed of a cold-resistant material and sealed at one end, which is detachably attached to the main body 2 so that the egg attachment holding strip 3 can be encapsulated. In particular, the tubular member 4 of this embodiment is sealed so as to be ventilated by a cotton-like body 12 inserted inside one end side, and the other end portion is a tapered portion whose diameter is increased in a tapered shape. And the cotton-like body 12 accommodated in the cylindrical member 4 is fixed by an adhesive 13.
The cylindrical member 4 has an inner diameter and a length that can be encapsulated without contacting the egg attachment holding strip 3. The cylindrical member 4 is provided with a tapered portion, so that the strip 3 can be easily inserted into the cylindrical member, and the cylindrical portion of the main body 2 can easily enter the cylindrical member. It is said.
As a forming material of the cylindrical member 4, for example, 3-fluorinated polyethylene, low density polyethylene, medium density polyethylene, high density polyethylene, polycarbonate, nylon, polysulfone, polyester, polystyrene, polyimide, ultrahigh molecular weight polyethylene, ethylene-acetic acid Examples thereof include a film made of a synthetic resin such as a vinyl copolymer or a laminate thereof. In particular, a liquid nitrogen resistant material, in other words, a material that does not become brittle when it comes into contact with liquid nitrogen is preferable.
[0012]
Next, the cylindrical member holding device of the present invention will be described with reference to the embodiments shown in the drawings.
FIG. 6 is a front view of the tubular member holding device of the present invention. FIG. 7 is a partially enlarged view of the distal end portion of the cylindrical member holding device shown in FIG. 6 in a released state.
The tubular member holding device 20 of the present invention includes two holding members 21 and 22 each having a holding portion 25 and 26 on the front end side and gripping portions 23 and 24 on the rear end side. This is a scissors forceps-like tubular member holding device that is pivotally supported between the holding portions 25 and 26 and the gripping portions 23 and 24. The opposing holding portions 25 and 26 of the two holding members 21 and 22 are formed with a pair of opposing recesses 31 and 32 for holding the small-diameter cylindrical member without being substantially crushed.
This cylindrical member holding device is effectively used for holding the cylindrical member 4 of the egg cryopreservation tool described above.
[0013]
The cylindrical member holding device 20 of the present invention includes two holding members 21 and 22, which are substantially symmetrical. The holding member 21 includes a clamping part 25 on the front end side and a gripping part 23 on the rear end side. Similarly, the holding member 22 includes a clamping part 26 on the front end side and a gripping part 24 on the rear end side. The sandwiching portions 25 and 26 are provided with facing blade portions 27 and 28 and a pair of facing recesses 31 and 32 provided at the tip portions of the sandwiching portions 25 and 26. The two holding members 21 and 22 are pivotally supported by a shaft 29 between the holding portions 25 and 26 and the gripping portions 23 and 24. And the recessed parts 31 and 32 are the magnitude | sizes which can hold | maintain, without crushing a small diameter cylindrical member, when the cylindrical member 4 is clamped in the said recessed part 31 and 32 part of the cylindrical member holding instrument 20. FIG. Is formed. Specifically, it is formed so that the inner diameter of the circular opening constituted by the recesses 31 and 32 is slightly smaller than the outer diameter of the cylindrical member held.
[0014]
Furthermore, as shown in FIGS. 6 and 8, the cylindrical member holding device includes holding state maintaining mechanisms 35 and 36 provided on the two holding members 21 and 22 for maintaining the state of holding the cylindrical member. I have. Specifically, plate-like protrusions 35 and 36 extending in the direction of the other holding member from the vicinity of the holding parts 23 and 24 of the two holding members 21 and 22 are provided. As shown in FIG. 8, a stepwise engageable portion having a sawtooth cross section is formed. For this reason, in a state where the cylindrical member is clamped in the recessed portion of the cylindrical member holding device 20, when the gripping portions 23, 24 of the holding members 21, 22 are pushed in the approaching direction, the cross sections of the holding state maintaining mechanisms 35, 36 The saw blade portion is engaged and maintained in that state. For this reason, in this cylindrical member holding device 20, it is not necessary to apply a special force to maintain the cylindrical member in the holding state, and the cylindrical member 4 is encapsulated with the strip 3, and Installation is easy. Further, the engagement of the holding state maintaining mechanisms 35 and 36 can be released by spreading the two holding members 21 and 22 in the horizontal direction, in other words, in the vertical direction in FIG.
The cylindrical member holding device is made of a metal such as stainless steel or titanium alloy.
[0015]
Next, the usage method of the egg cryopreservation tool 1 and the cylindrical member holding device 20 of the present invention will be described.
In this description, the case where an egg is stored frozen will be described as an example.
First, an ovum is collected at the tip of the pipette, an operation of replacing the ovum's intracellular solution with an equilibrium solution, and an operation of replacing the extracellular solution with a vitrification solution. Then, under the microscope, the ovum is attached to the tip of the egg adhesion holding strip 3 of the egg cryopreservation tool 1 of the present invention together with a small amount of vitrification solution, and the strip portion to which the ovum has adhered is prepared in advance. Immerse in nitrogen and freeze (vitrify). And after attaching the cylindrical member 4 to the main body member 10 using the cylindrical member holding fixture which hold | maintains the cylindrical member 4 prepared beforehand and the state is maintained, and after removing the cylindrical member holding fixture After storing the egg cryopreservation tool for adhering and holding the vitrified ovum in the storage container (cane), the storage container is stored in a liquid nitrogen tank.
[0016]
【The invention's effect】
The egg cryopreservation device of the present invention includes a main body portion formed of a cold-resistant material, and an egg adhesion holding strip attached to one end of the main body portion and formed of a flexible, transparent and liquid nitrogen-resistant material. One end of the egg attachment / removal strip detachably attached to the main body portion is sealed, and is formed of a cylindrical member formed of a cold-resistant material.
By providing the egg adhesion holding strip, it is easy to adhere and hold an egg such as an ovum or embryo together with the vitrification solution, and it is also possible to easily immerse the egg in liquid nitrogen.
[Brief description of the drawings]
FIG. 1 is a front view of an egg cryopreservation device according to an embodiment of the present invention.
2 is a front view of a state in which a cylindrical member of the egg freezing preservation tool shown in FIG. 1 is detached from a main body member. FIG.
FIG. 3 is a side view of a main body member of the egg freezing preservation tool shown in FIG. 2;
4 is an enlarged front view of a distal end portion of a main body member and a tubular member of the egg cryopreservation tool shown in FIG. 1. FIG.
FIG. 5 is an enlarged cross-sectional view of the distal end portion of the main body member and the cylindrical member of the egg cryopreservation tool shown in FIG.
FIG. 6 is a front view of the cylindrical member holding device of the present invention.
FIG. 7 is an enlarged view of the distal end portion of the cylindrical member holding device shown in FIG. 6 in a released state.
FIG. 8 is an explanatory view for explaining a holding state maintaining mechanism of the cylindrical member holding device of the present invention.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Egg cryopreservation tool 2 Body part 3 Egg adhesion holding strip 4 Tubular member

Claims (8)

液体窒素耐性材料により形成された本体部と、該本体部の一端に取り付けられ、可撓性かつ透明性かつ液体窒素耐性材料により形成された卵付着保持用ストリップと、該卵付着保持用ストリップを被包可能に前記本体部に着脱自在に取り付けられる一端が封鎖され、かつ液体窒素耐性材料により形成された筒状部材とからなることを特徴とする卵凍結保存用具。A body portion formed by a liquid nitrogen-resistant material, attached to one end of the body portion, and egg deposition holding strip formed by a flexible and transparent and liquid nitrogen-resistant material, the egg deposition retaining strip An egg cryopreservation tool comprising: a cylindrical member formed of a liquid nitrogen-resistant material and having one end removably attached to the main body so as to be encapsulated. 前記本体部は、細長い平板状本体部である請求項１に記載の卵凍結保存用具。  The egg cryopreservation tool according to claim 1, wherein the main body is an elongated flat plate main body. 前記卵付着保持用ストリップは、細長い平坦フィルム状の卵付着保持用ストリップである請求項１または２に記載の卵凍結保存用具。  3. The egg cryopreservation device according to claim 1, wherein the egg adhesion holding strip is an elongated flat film-like egg adhesion holding strip. 前記筒状部材は、一端側内部に挿入された綿状体により通気可能に封止されている請求項１ないし３のいずれかに記載の卵凍結保存用具。  The egg cryopreservation tool according to any one of claims 1 to 3, wherein the cylindrical member is sealed so as to be ventilated by a cotton-like body inserted into one end side. 前記本体部は、前記卵付着保持用ストリップの一端を保持するとともに前記筒状部材の他端内に侵入し係合する円柱状部を備えている請求項１ないし４のいずれかに記載の卵凍結保存用具。  The egg according to any one of claims 1 to 4, wherein the main body portion includes a columnar portion that holds one end of the strip for attaching and holding the egg and enters and engages the other end of the cylindrical member. Cryopreservation tool. 前記筒状部材は、他端部がテーパー状に拡径するテーパー部となっている請求項１ないし５のいずれかに記載の卵凍結保存用具。  The egg cryopreservation tool according to any one of claims 1 to 5, wherein the tubular member has a tapered portion whose other end portion has a tapered diameter. 前記請求項１ないし６のいずれかに記載の卵凍結保存用具の前記筒状部材を保持するための筒状部材保持器具であって、該筒状部材保持器具は、先端側に挟持部を備え、後端側に把持部を備える２つの保持部材からなり、該２つの保持部材の前記挟持部と前記把持部間において回動自在に軸支された鋏鉗子状の筒状部材保持器具であって、前記２つの保持部材の向かい合う挟持部には、細径の筒状部材を実質的に潰すことなく保持するために向かい合う一組の凹部が形成されていることを特徴とする筒状部材保持器具。 A cylindrical member holding device for holding the cylindrical member of the egg cryopreservation device according to any one of claims 1 to 6, wherein the cylindrical member holding device includes a clamping portion on a distal end side. The scissors-shaped cylindrical member holding device is composed of two holding members each having a gripping portion on the rear end side, and is pivotally supported between the gripping portion and the gripping portion of the two holding members. A pair of concave portions facing each other to hold the narrow cylindrical member without being substantially crushed is formed in the sandwiching portions of the two holding members facing each other. Instruments. 前記筒状部材保持器具は、前記筒状部材を保持した状態を維持するための保持状態維持機構を備えている請求項７に記載の筒状部材保持器具。  The said cylindrical member holding fixture is a cylindrical member holding fixture of Claim 7 provided with the holding | maintenance state maintenance mechanism for maintaining the state which hold | maintained the said cylindrical member.
JP2001120225A 2001-04-18 2001-04-18 Egg cryopreservation tool and cylindrical member holding device Expired - Lifetime JP4373025B2 (en)

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