CN103202285B - A kind of cell freezing save set - Google Patents
A kind of cell freezing save set Download PDFInfo
- Publication number
- CN103202285B CN103202285B CN201210006712.5A CN201210006712A CN103202285B CN 103202285 B CN103202285 B CN 103202285B CN 201210006712 A CN201210006712 A CN 201210006712A CN 103202285 B CN103202285 B CN 103202285B
- Authority
- CN
- China
- Prior art keywords
- protective casing
- loaded member
- core bar
- cell freezing
- save set
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000007710 freezing Methods 0.000 title claims abstract description 31
- 230000008014 freezing Effects 0.000 title claims abstract description 31
- 230000001681 protective effect Effects 0.000 claims abstract description 53
- 238000007789 sealing Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000006727 cell loss Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
Abstract
The present invention relates to cell freezing technical field, a kind of cell freezing save set is specifically provided.Its structure comprises protective casing, core bar, loaded member and the pendant part that hangs down; described protective casing one end is closed; the pendant part that hangs down is fixed on the blind end of protective casing; core bar is placed in protective casing; loaded member is fixed on core bar one end near protective casing blind end; be characterized in, described loaded member is sheet, has spacing hole in the middle part of loaded member.Compared with prior art, cell freezing save set of the present invention has easy to use, cell freezing success rate high.
Description
Technical field
The present invention relates to cell freezing technical field, a kind of cell freezing save set is specifically provided.
Background technology
Along with the development of the biotechnology such as supplementary reproduction, clone, the technology of glass freezing biological cell is widely used.
Vitrificated cryopreserration device under prior art is formed primarily of protective casing, core bar, loaded member and the pendant part that hangs down.On the one hand, described loaded member is generally the freezing shovel of the freezing hook of " J " type, ring-type nylon ring or sheet, has the following disadvantages: one in application process, and freezing liquid amount ratio is comparatively large, affects the freezing rate of cell; Two, the dielectric layer that freezing liquid is formed, easily because misoperation is broken, causes cell loss.On the other hand, before thawing, the core bar in protective casing not easily takes out, and uses inconvenience.
Summary of the invention
The present invention be directed to above-mentioned the deficiencies in the prior art, a kind of reasonable in design, easy to use, cell freezing save set that cell freezing success rate is high is provided.
The technical solution adopted for the present invention to solve the technical problems is: a kind of cell freezing save set; comprise protective casing, core bar, loaded member and the pendant part that hangs down; described protective casing one end is closed; the pendant part that hangs down is fixed on the blind end of protective casing; core bar is placed in protective casing, and loaded member is fixed on core bar one end near protective casing blind end, is characterized in; described loaded member is sheet, has spacing hole in the middle part of loaded member.
In precooling process, protective casing produces flexural deformation, and freezing storage device of the present invention further comprises support bar, and support bar can plug with protective casing.During precooling, support bar is inserted in protective casing, protective casing can be avoided to produce low temperature deformation.
Conveniently core bar takes out, and can arrange taking-up handle at core bar near the position of protective casing openend, and on protective casing, corresponding position of taking out handle is provided with otch graticule.
The cross section of described loaded member is preferably in " [" shape, to increase the intensity of loaded member.
The operation hand-held quality award from the ministry choosing of described core bar arranges anti-skidding tangent plane, to increase stability in operating process.
The openend of described protective casing preferably arranges sealing graticule, in order to the best seal location of alert protective casing.
Described protective casing preferably arranges security warning graticule near the position of loaded member, in order to the position of alert loaded member, avoids the cell loss of artificial touching in operating process and generation.
Compared to the prior art cell freezing save set of the present invention, has following outstanding beneficial effect:
(1) freezing liquid consumption is few, effectively can improve freezing rate, reinforced glass solidification;
(2) spacing hole has certain position-limiting action to biological cell, can avoid the cell loss because misoperation causes;
(3) after protective casing cuts by cut line position, directly can utilize and take out handle by core bar together with loaded member and cell taking-up, easy to use, operating efficiency is high.
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of cell freezing save set of the present invention;
Accompanying drawing 2 is A-A cutaway views of freezing storage device shown in Fig. 1;
Accompanying drawing 3 is B-B cutaway views of freezing storage device shown in Fig. 1.
Embodiment
Below in conjunction with the drawings and specific embodiments, the invention will be further described, but not as a limitation of the invention.
Provide a most preferred embodiment below:
A kind of cell freezing save set, is made up of protective casing 1, core bar 2, loaded member 3, vertical pendant part 4 and support bar 5.
Described protective casing 1 for one end close, the body of open at one end.
The pendant part 4 that hangs down is fixed on the blind end of protective casing 1, the wire netting larger for proportion or metal derby.
Described core bar 2 is placed in protective casing 1.In the loaded member 3 that core bar 2 is fixing near one end of protective casing 1 blind end, be fixed with taking-up handle 6 at core bar 2 near one end of protective casing 1 open end, the operation Handheld Division of core bar 2 is provided with anti-skidding tangent plane 11.
Described loaded member 3 is for cross section is in " the sheet of [" shape.Spacing hole 7 is had at the middle part of loaded member 3.
Protective casing 1 indicates sealing graticule 8, otch graticule 9 and security warning graticule 10 from openend successively to blind end.Sealing graticule 8 is located at the openend of protective casing 1, in order to the best seal location of alert protective casing 1; Otch graticule 9 is located at corresponding core bar on protective casing 1 and takes out the position of handle 6, and the best for alert protective casing 1 cuts position; Security warning graticule 10 is located at the position of protective casing 1 near loaded member 3, in order to the position of alert loaded member, avoids the cell loss of artificial touching in operating process and generation.
Described support bar 5 is stiff shaft, can plug with protective casing 1.
The using method of cell freezing save set of the present invention is as follows:
1, support bar 5 is put into protective casing 1, and protective casing 1 is put into liquid nitrogen bath precooling, for subsequent use;
2, the media fluid of cell there is is to put in the spacing hole 7 of loaded member 3 suction under the microscope;
3, the support bar in protective casing 1 is taken out, and the core bar 2 being placed with cell is put in protective casing 1;
4, the opening end of closed protective sleeve pipe 1, and protective casing 1 is put into liquid nitrogen container freezen protective.
When needing to take out frozen cell, utilize scissors (or other cutting implement) to be cut off by protective casing 1, hold taking-up handle 6 and core bar 2 is taken out together with cell.
Claims (5)
1. a cell freezing save set, comprise protective casing, core bar, loaded member and the pendant part that hangs down, described protective casing one end is closed; the pendant part that hangs down is fixed on the blind end of protective casing, and core bar is placed in protective casing, and loaded member is fixed on core bar one end near protective casing blind end; it is characterized in that
Described loaded member is sheet, has spacing hole in the middle part of loaded member, and the cross section of loaded member is in " [" shape;
Be provided with support bar, support bar can plug with protective casing.
2. cell freezing save set according to claim 1, is characterized in that, the operation Handheld Division of described core bar is provided with anti-skidding tangent plane.
3. cell freezing save set according to claim 1 and 2, is characterized in that, core bar is provided with taking-up handle near the position of protective casing openend, and on protective casing, corresponding position of taking out handle is provided with otch graticule.
4. cell freezing save set according to claim 1 and 2, is characterized in that, the openend of described protective casing is provided with sealing graticule.
5. cell freezing save set according to claim 1 and 2, is characterized in that, described protective casing is provided with security warning graticule near the position of loaded member.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210006712.5A CN103202285B (en) | 2012-01-11 | 2012-01-11 | A kind of cell freezing save set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210006712.5A CN103202285B (en) | 2012-01-11 | 2012-01-11 | A kind of cell freezing save set |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103202285A CN103202285A (en) | 2013-07-17 |
| CN103202285B true CN103202285B (en) | 2015-11-25 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210006712.5A Active CN103202285B (en) | 2012-01-11 | 2012-01-11 | A kind of cell freezing save set |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103202285B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396945A (en) * | 2014-12-03 | 2015-03-11 | 张丽红 | Spermatozoa cryopreservation and thawing method for detecting sperm DNA (Deoxyribonucleic Acid) integrity |
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| JP2001252293A (en) * | 2000-03-14 | 2001-09-18 | Hyogo Prefecture | Tool and method for vitrifying ovum or embryo |
| JP2002315573A (en) * | 2001-04-18 | 2002-10-29 | Kitazato Supply:Co Ltd | Tool for storing frozen egg and cylindrical member- retaining instrument |
| CN1774501A (en) * | 2003-04-15 | 2006-05-17 | 株式会社北里萨普利 | Implement and method for freezing and storing egg |
| CN101386814A (en) * | 2008-10-21 | 2009-03-18 | 北京大学第三医院 | Biological material vitrified frozen vector and preparation method thereof |
| CN100546721C (en) * | 2004-12-21 | 2009-10-07 | 麦吉尔大学 | The tool and method of operation developmental cells sample in process of cryopreservation |
| CN201617121U (en) * | 2010-03-18 | 2010-11-03 | 安徽农业大学 | Biological material vitrification carrier |
| EP2353383A2 (en) * | 2010-02-09 | 2011-08-10 | Enrique Criado Scholz | Closed ultra-rapid cell vitrification device and sealing procedure of the device |
| CN102204527A (en) * | 2011-04-15 | 2011-10-05 | 山东威高新生医疗器械有限公司 | Carrying tool for use in vitrification cryo-preservation of biomaterial |
| CN202095441U (en) * | 2011-04-25 | 2012-01-04 | 石家庄市第四医院 | Biological material vitrification freezing carrier |
| CN202436011U (en) * | 2012-01-11 | 2012-09-19 | 魏毓君 | Cell cryopreservation device |
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2012
- 2012-01-11 CN CN201210006712.5A patent/CN103202285B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001252293A (en) * | 2000-03-14 | 2001-09-18 | Hyogo Prefecture | Tool and method for vitrifying ovum or embryo |
| JP2002315573A (en) * | 2001-04-18 | 2002-10-29 | Kitazato Supply:Co Ltd | Tool for storing frozen egg and cylindrical member- retaining instrument |
| CN1774501A (en) * | 2003-04-15 | 2006-05-17 | 株式会社北里萨普利 | Implement and method for freezing and storing egg |
| CN100546721C (en) * | 2004-12-21 | 2009-10-07 | 麦吉尔大学 | The tool and method of operation developmental cells sample in process of cryopreservation |
| CN101386814A (en) * | 2008-10-21 | 2009-03-18 | 北京大学第三医院 | Biological material vitrified frozen vector and preparation method thereof |
| EP2353383A2 (en) * | 2010-02-09 | 2011-08-10 | Enrique Criado Scholz | Closed ultra-rapid cell vitrification device and sealing procedure of the device |
| CN201617121U (en) * | 2010-03-18 | 2010-11-03 | 安徽农业大学 | Biological material vitrification carrier |
| CN102204527A (en) * | 2011-04-15 | 2011-10-05 | 山东威高新生医疗器械有限公司 | Carrying tool for use in vitrification cryo-preservation of biomaterial |
| CN202095441U (en) * | 2011-04-25 | 2012-01-04 | 石家庄市第四医院 | Biological material vitrification freezing carrier |
| CN202436011U (en) * | 2012-01-11 | 2012-09-19 | 魏毓君 | Cell cryopreservation device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103202285A (en) | 2013-07-17 |
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Owner name: SHANDONG AIWEIFU BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: WEI YUJUN Effective date: 20150611 |
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Inventor after: Meng Ronghua Inventor before: Wei Yujun |
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Effective date of registration: 20161124 Address after: Fu Road, Licheng District, Shandong city of Ji'nan Province, No. 5 250108 Patentee after: Pacific Kangtai scientific instrument (Ji'nan) Co., Ltd. Address before: 250101, Ji'nan hi tech Zone, Shandong, No. Hi Road, building 1-101-527 Patentee before: SHANDONG AIWEIFU BIOTECHNOLOGY CO., LTD. |
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Denomination of invention: Cell cryopreservation apparatus Effective date of registration: 20200117 Granted publication date: 20151125 Pledgee: Qilu bank Limited by Share Ltd Ji'nan science and technology innovation center sub branch Pledgor: Pacific Kangtai scientific instrument (Ji'nan) Co., Ltd. Registration number: Y2020370000011 |
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Date of cancellation: 20220304 Granted publication date: 20151125 Pledgee: Qilu bank Limited by Share Ltd. Ji'nan science and technology innovation center sub branch Pledgor: TAIPINGYANG KANGTAI SCIENTIFIC APPARATUS (JINAN) CO.,LTD. Registration number: Y2020370000011 |