JP4340734B2 - 細胞検出方法 - Google Patents

細胞検出方法 Download PDF

Info

Publication number
JP4340734B2
JP4340734B2 JP2001575233A JP2001575233A JP4340734B2 JP 4340734 B2 JP4340734 B2 JP 4340734B2 JP 2001575233 A JP2001575233 A JP 2001575233A JP 2001575233 A JP2001575233 A JP 2001575233A JP 4340734 B2 JP4340734 B2 JP 4340734B2
Authority
JP
Japan
Prior art keywords
nucleic acid
sample
life
cells
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2001575233A
Other languages
English (en)
Japanese (ja)
Other versions
JP2003530118A5 (enExample
JP2003530118A (ja
Inventor
ルディ、ノット
Original Assignee
マトフォルスク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by マトフォルスク filed Critical マトフォルスク
Publication of JP2003530118A publication Critical patent/JP2003530118A/ja
Publication of JP2003530118A5 publication Critical patent/JP2003530118A5/ja
Application granted granted Critical
Publication of JP4340734B2 publication Critical patent/JP4340734B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2001575233A 2000-04-10 2001-04-10 細胞検出方法 Expired - Lifetime JP4340734B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0008787.4 2000-04-10
GBGB0008787.4A GB0008787D0 (en) 2000-04-10 2000-04-10 A method of cell detection
PCT/GB2001/001619 WO2001077379A2 (en) 2000-04-10 2001-04-10 A method of cell detection

Publications (3)

Publication Number Publication Date
JP2003530118A JP2003530118A (ja) 2003-10-14
JP2003530118A5 JP2003530118A5 (enExample) 2005-06-09
JP4340734B2 true JP4340734B2 (ja) 2009-10-07

Family

ID=9889587

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001575233A Expired - Lifetime JP4340734B2 (ja) 2000-04-10 2001-04-10 細胞検出方法

Country Status (8)

Country Link
US (1) US7262009B2 (enExample)
EP (1) EP1272664A2 (enExample)
JP (1) JP4340734B2 (enExample)
AU (2) AU2001246712C1 (enExample)
CA (1) CA2405769A1 (enExample)
GB (1) GB0008787D0 (enExample)
NO (1) NO20024879L (enExample)
WO (1) WO2001077379A2 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1769081B1 (en) 2004-07-01 2016-01-20 Litron Laboratories Ltd. Method for enumerating mammalian cell micronuclei with an emphasis on differentially staining micronuclei and the chromatin of dead and dying cells
JP4127846B2 (ja) 2005-07-21 2008-07-30 国立大学法人九州大学 微生物検出法及び微生物検出キット
EP1992690B1 (en) 2006-02-17 2014-05-21 Morinaga Milk Industry Co., Ltd. Method for detection of microorganism and kit for detection of microorganism
PL2004843T3 (pl) * 2006-02-28 2015-12-31 Univ Montana State Zastosowanie pochodnych fenantrydyny dla rozróżniania komórek nienaruszonych i komórek z uszkodzoną błoną za pomocą technik molekularnych opartych o kwas nukleinowy
JP4113556B2 (ja) * 2006-12-04 2008-07-09 国立大学法人九州大学 核酸分解剤および核酸の分解方法
CA2666448C (en) * 2007-08-16 2013-09-10 Morinaga Milk Industry Co., Ltd. Method and kit for the detection of live microorganism cells in a sample
CN103820578B (zh) 2009-07-24 2015-06-17 森永乳业株式会社 用于微生物检测的方法及试剂盒
WO2011043737A1 (en) * 2009-10-05 2011-04-14 Nanyang Technological University Viability analysis of protozoa using polymerase chain reaction (pcr)
FR2974117B1 (fr) 2011-04-18 2018-05-11 Societe Des Eaux De Marseille Procede de quantification de bacteries vivantes dans un milieu liquide
US9709500B2 (en) 2012-05-02 2017-07-18 Charles River Laboratories, Inc. Optical method for detecting viable microorganisms in a cell sample
US8993260B2 (en) 2012-05-02 2015-03-31 Charles River Laboratories, Inc. Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher
EP2769204B1 (en) 2012-05-02 2016-02-17 Charles River Laboratories, Inc. Cell capture system and use thereof
DE102020103971B4 (de) * 2020-02-14 2022-02-24 Testo bioAnalytics GmbH Verfahren zum Nachweis von lebenden Mikroorganismen und ein fluidisches Kanalsystem

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0517154A1 (en) * 1991-06-06 1992-12-09 F. Hoffmann-La Roche Ag Method for detecting giardia
US5314805A (en) * 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
US5525471A (en) * 1994-10-12 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enzymatic degrading subtraction hybridization
WO1997024616A1 (fr) 1995-12-29 1997-07-10 Ishihara Sangyo Kaisha, Ltd. Procede de determination du taux de viabilite de cellules
US5780278A (en) * 1996-05-20 1998-07-14 Vanderbilt University IceA gene and related methods
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method

Also Published As

Publication number Publication date
US20030203374A1 (en) 2003-10-30
CA2405769A1 (en) 2001-10-18
EP1272664A2 (en) 2003-01-08
US7262009B2 (en) 2007-08-28
GB0008787D0 (en) 2000-05-31
AU2001246712B2 (en) 2006-10-19
AU4671201A (en) 2001-10-23
WO2001077379A3 (en) 2002-06-20
WO2001077379A2 (en) 2001-10-18
NO20024879D0 (no) 2002-10-09
NO20024879L (no) 2002-12-10
AU2001246712C1 (en) 2007-08-02
JP2003530118A (ja) 2003-10-14

Similar Documents

Publication Publication Date Title
Birch et al. A comparison of nucleic acid amplification techniques for the assessment of bacterial viability
Perry‐O’Keefe et al. Filter‐based PNA in situ hybridization for rapid detection, identification and enumeration of specific micro‐organisms
JP4340734B2 (ja) 細胞検出方法
US20220372558A1 (en) Method and Kit of Detecting the Absence of Micro-Organisms
EP3775254B1 (en) Microorganism separation and detection
JPS60501339A (ja) 生物を検出、同定又は定量する方法およびキット
CN112159854A (zh) 一种大肠杆菌O157:H7的CRISPR/Cas12a检测用引物组合物和检测方法
AU2001246712A1 (en) A method of cell detection
KR101609224B1 (ko) 핵산 변형 방법
Huang et al. Flow cytometry-based method facilitates optimization of PMA treatment condition for PMA-qPCR method
Giacomazzi et al. Comparison of three methods of DNA extraction from cold‐smoked salmon and impact of physical treatments
US11851705B2 (en) Microarray based multiplex pathogen analysis for plants, agriculture, food, and water
Petershofen et al. Detection of nucleic acid sequences from bacterial species with molecular genetic methods
EP1851325B1 (en) Method for detecting viable cells in a sample by using a virus
JP3507692B2 (ja) ヌクレオチド配列標的アナライトの検出用集合体並びに検出及び定量方法
WO2005083114A1 (en) Method, kit and system for enhanced nested pcr
Call et al. Using DNA suspension arrays to identify library-independent markers for bacterial source tracking
JP4765058B2 (ja) 核酸修飾前の検体の前処理方法
RU2738358C1 (ru) Набор олигонуклеотидных праймеров и флуоресцентно-меченых зондов и способ выявления ДНК возбудителей сапа и мелиоидоза методом ПЦР с детекцией продукта в режиме реального времени
WO2024025481A1 (en) A crispr rna for detection of burkholderia pseudomallei-associated genetic material in a biological sample and a method using the same
WO2024048755A1 (ja) 迅速無菌試験法
US20210164022A1 (en) Assay for detection of pathogenic leptospira strains
CN120418448A (zh) 用于测定生物来源样品中可萌发细胞的数量和/或浓度的方法
KR101773657B1 (ko) 산화칼슘을 이용한 시료 전처리 방법
JP2015119656A (ja) 核酸増幅法

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20040506

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20060725

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20061024

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20061031

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070124

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20081021

RD13 Notification of appointment of power of sub attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7433

Effective date: 20081226

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090121

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20081226

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20090512

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20090604

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120717

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Ref document number: 4340734

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120717

Year of fee payment: 3

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120717

Year of fee payment: 3

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130717

Year of fee payment: 4

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term