CA2405769A1 - A method of distinguishing between living and dead cells using viability probes - Google Patents
A method of distinguishing between living and dead cells using viability probes Download PDFInfo
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- CA2405769A1 CA2405769A1 CA002405769A CA2405769A CA2405769A1 CA 2405769 A1 CA2405769 A1 CA 2405769A1 CA 002405769 A CA002405769 A CA 002405769A CA 2405769 A CA2405769 A CA 2405769A CA 2405769 A1 CA2405769 A1 CA 2405769A1
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- Organic Chemistry (AREA)
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- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0008787.4 | 2000-04-10 | ||
| GBGB0008787.4A GB0008787D0 (en) | 2000-04-10 | 2000-04-10 | A method of cell detection |
| PCT/GB2001/001619 WO2001077379A2 (en) | 2000-04-10 | 2001-04-10 | A method of cell detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2405769A1 true CA2405769A1 (en) | 2001-10-18 |
Family
ID=9889587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002405769A Abandoned CA2405769A1 (en) | 2000-04-10 | 2001-04-10 | A method of distinguishing between living and dead cells using viability probes |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7262009B2 (enExample) |
| EP (1) | EP1272664A2 (enExample) |
| JP (1) | JP4340734B2 (enExample) |
| AU (2) | AU2001246712C1 (enExample) |
| CA (1) | CA2405769A1 (enExample) |
| GB (1) | GB0008787D0 (enExample) |
| NO (1) | NO20024879L (enExample) |
| WO (1) | WO2001077379A2 (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8993259B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Method of viability staining with membrane permeable fluorescent dye and membrane impermeable fluorescence quencher |
| US9709500B2 (en) | 2012-05-02 | 2017-07-18 | Charles River Laboratories, Inc. | Optical method for detecting viable microorganisms in a cell sample |
| US10324036B2 (en) | 2012-05-02 | 2019-06-18 | Charles River Laboratories, Inc. | Porous planar cell capture system |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1769081B1 (en) | 2004-07-01 | 2016-01-20 | Litron Laboratories Ltd. | Method for enumerating mammalian cell micronuclei with an emphasis on differentially staining micronuclei and the chromatin of dead and dying cells |
| JP4127846B2 (ja) | 2005-07-21 | 2008-07-30 | 国立大学法人九州大学 | 微生物検出法及び微生物検出キット |
| EP1992690B1 (en) | 2006-02-17 | 2014-05-21 | Morinaga Milk Industry Co., Ltd. | Method for detection of microorganism and kit for detection of microorganism |
| PL2004843T3 (pl) * | 2006-02-28 | 2015-12-31 | Univ Montana State | Zastosowanie pochodnych fenantrydyny dla rozróżniania komórek nienaruszonych i komórek z uszkodzoną błoną za pomocą technik molekularnych opartych o kwas nukleinowy |
| JP4113556B2 (ja) * | 2006-12-04 | 2008-07-09 | 国立大学法人九州大学 | 核酸分解剤および核酸の分解方法 |
| CA2666448C (en) * | 2007-08-16 | 2013-09-10 | Morinaga Milk Industry Co., Ltd. | Method and kit for the detection of live microorganism cells in a sample |
| CN103820578B (zh) | 2009-07-24 | 2015-06-17 | 森永乳业株式会社 | 用于微生物检测的方法及试剂盒 |
| WO2011043737A1 (en) * | 2009-10-05 | 2011-04-14 | Nanyang Technological University | Viability analysis of protozoa using polymerase chain reaction (pcr) |
| FR2974117B1 (fr) | 2011-04-18 | 2018-05-11 | Societe Des Eaux De Marseille | Procede de quantification de bacteries vivantes dans un milieu liquide |
| DE102020103971B4 (de) * | 2020-02-14 | 2022-02-24 | Testo bioAnalytics GmbH | Verfahren zum Nachweis von lebenden Mikroorganismen und ein fluidisches Kanalsystem |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0517154A1 (en) * | 1991-06-06 | 1992-12-09 | F. Hoffmann-La Roche Ag | Method for detecting giardia |
| US5314805A (en) * | 1991-10-28 | 1994-05-24 | Molecular Probes, Inc. | Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM |
| US5525471A (en) * | 1994-10-12 | 1996-06-11 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Enzymatic degrading subtraction hybridization |
| WO1997024616A1 (fr) | 1995-12-29 | 1997-07-10 | Ishihara Sangyo Kaisha, Ltd. | Procede de determination du taux de viabilite de cellules |
| US5780278A (en) * | 1996-05-20 | 1998-07-14 | Vanderbilt University | IceA gene and related methods |
| GB9709728D0 (en) | 1997-05-13 | 1997-07-02 | Dynal As | Single step method |
-
2000
- 2000-04-10 GB GBGB0008787.4A patent/GB0008787D0/en not_active Ceased
-
2001
- 2001-04-10 CA CA002405769A patent/CA2405769A1/en not_active Abandoned
- 2001-04-10 AU AU2001246712A patent/AU2001246712C1/en not_active Ceased
- 2001-04-10 AU AU4671201A patent/AU4671201A/xx active Pending
- 2001-04-10 US US10/257,493 patent/US7262009B2/en not_active Expired - Fee Related
- 2001-04-10 JP JP2001575233A patent/JP4340734B2/ja not_active Expired - Lifetime
- 2001-04-10 WO PCT/GB2001/001619 patent/WO2001077379A2/en not_active Ceased
- 2001-04-10 EP EP01919651A patent/EP1272664A2/en not_active Withdrawn
-
2002
- 2002-10-09 NO NO20024879A patent/NO20024879L/no unknown
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8993259B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Method of viability staining with membrane permeable fluorescent dye and membrane impermeable fluorescence quencher |
| US8993260B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher |
| US9709500B2 (en) | 2012-05-02 | 2017-07-18 | Charles River Laboratories, Inc. | Optical method for detecting viable microorganisms in a cell sample |
| US10324036B2 (en) | 2012-05-02 | 2019-06-18 | Charles River Laboratories, Inc. | Porous planar cell capture system |
| US10976258B2 (en) | 2012-05-02 | 2021-04-13 | Charles River Laboratories, Inc. | Porous planar cell capture system and method of use |
Also Published As
| Publication number | Publication date |
|---|---|
| US20030203374A1 (en) | 2003-10-30 |
| JP4340734B2 (ja) | 2009-10-07 |
| EP1272664A2 (en) | 2003-01-08 |
| US7262009B2 (en) | 2007-08-28 |
| GB0008787D0 (en) | 2000-05-31 |
| AU2001246712B2 (en) | 2006-10-19 |
| AU4671201A (en) | 2001-10-23 |
| WO2001077379A3 (en) | 2002-06-20 |
| WO2001077379A2 (en) | 2001-10-18 |
| NO20024879D0 (no) | 2002-10-09 |
| NO20024879L (no) | 2002-12-10 |
| AU2001246712C1 (en) | 2007-08-02 |
| JP2003530118A (ja) | 2003-10-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |
Effective date: 20121126 |