JP3905838B2 - 蛍光共鳴エネルギー転移（ｆｒｅｔ）測定用ダイペア - Google Patents
蛍光共鳴エネルギー転移（ｆｒｅｔ）測定用ダイペア Download PDFInfo
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- JP3905838B2 JP3905838B2 JP2002542878A JP2002542878A JP3905838B2 JP 3905838 B2 JP3905838 B2 JP 3905838B2 JP 2002542878 A JP2002542878 A JP 2002542878A JP 2002542878 A JP2002542878 A JP 2002542878A JP 3905838 B2 JP3905838 B2 JP 3905838B2
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- 238000005259 measurement Methods 0.000 title claims abstract description 52
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- -1 sulfo, carboxy Chemical group 0.000 claims description 15
- 238000012546 transfer Methods 0.000 claims description 15
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- 238000002372 labelling Methods 0.000 claims description 4
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 4
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
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- STWJKLMRMTWJEY-UHFFFAOYSA-N diphenyl 1,10-phenanthroline-4,7-disulfonate Chemical compound C=1C=NC(C2=NC=CC(=C2C=C2)S(=O)(=O)OC=3C=CC=CC=3)=C2C=1S(=O)(=O)OC1=CC=CC=C1 STWJKLMRMTWJEY-UHFFFAOYSA-N 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- IWBOPFCKHIJFMS-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl) ether Chemical compound NCCOCCOCCN IWBOPFCKHIJFMS-UHFFFAOYSA-N 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- 229910052741 iridium Inorganic materials 0.000 description 1
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
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- 150000007523 nucleic acids Chemical group 0.000 description 1
- 229910052762 osmium Inorganic materials 0.000 description 1
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
- 125000005010 perfluoroalkyl group Chemical group 0.000 description 1
- 230000010363 phase shift Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
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- WUAPFZMCVAUBPE-UHFFFAOYSA-N rhenium atom Chemical compound [Re] WUAPFZMCVAUBPE-UHFFFAOYSA-N 0.000 description 1
- 238000002821 scintillation proximity assay Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00124995 | 2000-11-16 | ||
| PCT/EP2001/013152 WO2002041001A1 (de) | 2000-11-16 | 2001-11-14 | Farbstoffpaare für fluoreszenz-resonanz-energie-transfer (fret) messungen |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004514139A JP2004514139A (ja) | 2004-05-13 |
| JP2004514139A5 JP2004514139A5 (enExample) | 2005-04-14 |
| JP3905838B2 true JP3905838B2 (ja) | 2007-04-18 |
Family
ID=8170391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002542878A Expired - Fee Related JP3905838B2 (ja) | 2000-11-16 | 2001-11-14 | 蛍光共鳴エネルギー転移（ｆｒｅｔ）測定用ダイペア |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6908769B2 (enExample) |
| EP (1) | EP1337851B1 (enExample) |
| JP (1) | JP3905838B2 (enExample) |
| AT (1) | ATE300050T1 (enExample) |
| AU (1) | AU2002221848A1 (enExample) |
| CA (1) | CA2426479C (enExample) |
| DE (1) | DE50106822D1 (enExample) |
| ES (1) | ES2245995T3 (enExample) |
| WO (1) | WO2002041001A1 (enExample) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE518913T1 (de) * | 2000-09-06 | 2011-08-15 | Evotec Ag | Verfahren zur gezielten markierung mittels affinitätsmarkierter detektionsmarker |
| AU2003251986A1 (en) * | 2002-07-17 | 2004-02-02 | U.S.Genomics, Inc. | Methods and compositions for analyzing polymers using chimeric tags |
| GB2421412B (en) * | 2003-02-26 | 2007-08-29 | Tomtom Bv | Navigation device with touch screen |
| CN100447145C (zh) * | 2003-08-18 | 2008-12-31 | 诺瓦提斯公司 | 适宜作为近红外显像剂的3h－吩嗪衍生物、其制备和用途 |
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| US5246867A (en) * | 1992-01-17 | 1993-09-21 | University Of Maryland At Baltimore | Determination and quantification of saccharides by luminescence lifetimes and energy transfer |
| US5622821A (en) * | 1994-06-29 | 1997-04-22 | The Regents Of The University Of California | Luminescent lanthanide chelates and methods of use |
| PT912766E (pt) * | 1996-06-04 | 2009-07-16 | Univ Utah Res Found | Monitorização da hibridização durante a pcr |
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| AU3487499A (en) | 1998-04-08 | 1999-10-25 | Regents Of The University Of California, The | Methods and reagents for targeting organic compounds to selected cellular locations |
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2001
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| ES2245995T3 (es) | 2006-02-01 |
| EP1337851A1 (de) | 2003-08-27 |
| ATE300050T1 (de) | 2005-08-15 |
| WO2002041001A1 (de) | 2002-05-23 |
| EP1337851B1 (de) | 2005-07-20 |
| US6908769B2 (en) | 2005-06-21 |
| DE50106822D1 (de) | 2005-08-25 |
| CA2426479C (en) | 2007-08-07 |
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