JP3547173B2 - Mite propagation method - Google Patents
Mite propagation method Download PDFInfo
- Publication number
- JP3547173B2 JP3547173B2 JP17687594A JP17687594A JP3547173B2 JP 3547173 B2 JP3547173 B2 JP 3547173B2 JP 17687594 A JP17687594 A JP 17687594A JP 17687594 A JP17687594 A JP 17687594A JP 3547173 B2 JP3547173 B2 JP 3547173B2
- Authority
- JP
- Japan
- Prior art keywords
- present
- humidity
- mites
- temperature
- propagation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Description
【0001】
【産業上の利用分野】
本発明は果菜類の重要害虫であるアザミウマ類、ハダニ類などの天敵であるカブリダニ(Amblyseius cucumeris;以下A.c.という)を生物農薬として有効利用するための大量増殖の技術に関するものである。
【0002】
【従来の技術】
従来、高品質の果菜を収穫していくために、果菜に発生する害虫や病害に対して、数種の殺虫剤や殺菌剤が体系的に高頻度で散布されているが、抵抗性害虫の出現や耐性の獲得などによって薬剤の効果も年々低下しつつあるのが実情である。
【0003】
一方こうした大量の薬剤散布について、人体への影響や環境汚染を懸念する声も高く、減農薬や、天敵を有効利用しようとする方法が各方面で研究されている。古くから、害虫に寄生したり、害虫を捕食したりする天敵は数多く知られているが、実際実用化するために必要な安定供給体制ができているものは少なく、あっても方法は公表されていない。
【0004】
【発明が解決しようとする課題】
本発明は果菜類の重要害虫であるアザミウマ類、ハダニ類の防除に有効なA.c.の大量増殖の方法を提供するものである。
【0005】
【課題を解決するための手段】
本発明者らは、A.c.を効率的に短期間で大量増殖するにあたって、種々の餌の混合比率を設定してA.c.が初期値(初期のA.c.の頭数)の例えば10〜300倍に増える系を検索した結果、A.c.数,餌昆虫数,温度及び湿度を特定の範囲に設定し、培地量を少なくすることによって、飛躍的に効率良く高い増殖効果を得る事を見出だし、本発明を完成するに至った。すなわち本発明はA.c.の増殖方法において、該A.c.数1頭に対して餌としてのコナダニを10〜500頭与え、かつ温度を15〜35℃及び湿度を80〜99%RHに設定することを特徴とするA.c.の増殖方法を提供する。
【0006】
以下本発明を詳細に説明する。
【0007】
本発明で天敵として用いるA.c.は公知の種属であって、例えばカナダのApplied Bionomic社やオランダのKOPPERT社などで、市販されており、容易に入手できるものである。
【0008】
次に、本発明の増殖法に用いる、A.c.の餌となるべきコナダニとしては例えば、ケナガコナダニ、アシブトコナダニ、チビコナダニ、コウノホシカダニ、ムギコナダニがあげられるが、これらに限定されるものではない。
【0009】
次に本発明の増殖法においては、A.c.1頭に対して、餌として与えるコナダニは10〜500頭であるが、好ましくは20〜100頭である。
【0010】
本発明の増殖法においては、増殖の際その環境の温度、湿度を適宜選択することができ通常は温度が15〜35℃、湿度が80〜99%の範囲であるが、好ましくは温度が20〜30℃、湿度は90〜96%の範囲である。
【0011】
本発明の増殖方法は、種々の容器内で行うことができるが、その容器の形状は特に限定されずに用いることができる。例えば、500mlの円柱状のプラスチックボトル(広口)を用いた場合、好ましくは20〜40gの培地中A.c.10〜100頭に対し、餌としてのコナダニを例えば2000〜5000頭与え、前期の温度、湿度条件で増殖することにより、数週間後には15000〜25000頭のA.c.を得ることができる。培地としては、バ−ミキュライト、もみ殻、ふすま、乾燥酵母などがあげられる。
【0012】
本発明を実施するに際して重要なことは、何頭のA.c.を何時までに供給してほしいという需要家の条件に合わせることであって、そのためには本発明の範囲内でA.c.の頭数(初期の頭数)、温度、湿度を選択することにより需要家の希望する頭数を希望する時期に供給することができる。
【0013】
【実施例】
以下実施例により本発明を説明する。
【0014】
実施例 1
500ml容量のポリボトルにふすまと乾燥酵母を合わせて50g入れ、温度を30℃、湿度95%RHの条件に静置したところに、5000頭のケナガコナダニとA.c.100頭を加えると、2週間後に20000頭のA.c.を得る事ができた。
【0015】
実施例 2
500ml容量のポリボトルにバ−ミキュライトと乾燥酵母を合わせて50g入れ、温度20℃、湿度95%RHの条件に静置したところに、2000頭のケナガコナダニとA.c.100頭を加えると、6週間後に20000頭のA.c.を得る事ができた。
【0016】
実施例3
500ml容量のポリボトルにもみ殻と乾燥酵母を合わせて50g入れ、温度25℃、湿度95%RHの条件に静置したところに、500頭のケナガコナダニとA.c.10頭を加えると、4週間後に2000頭のA.c.を得る事ができた。
【0017】
【発明の効果】
本発明により、A.c.の個体数を飛躍的に増加することができる。[0001]
[Industrial applications]
TECHNICAL FIELD The present invention relates to a technique of mass propagation for effectively utilizing Amblyseius cucumeris (hereinafter referred to as Ac), which is a natural enemy such as thrips and spider mites, which are important pests of fruits and vegetables, as biological pesticides.
[0002]
[Prior art]
Conventionally, in order to harvest high-quality fruits and vegetables, several insecticides and fungicides are systematically and frequently sprayed against pests and diseases that occur in fruits and vegetables. The fact is that the effects of drugs have been decreasing year by year due to the emergence and acquisition of tolerance.
[0003]
On the other hand, there has been a lot of concern about the impact on the human body and environmental pollution of such a large amount of spraying of chemicals, and methods for reducing the use of pesticides and effective use of natural enemies are being studied in various fields. Many natural enemies that parasitize or prey on pests have been known for a long time, but there are few that have a stable supply system necessary for practical use. Not.
[0004]
[Problems to be solved by the invention]
INDUSTRIAL APPLICABILITY The present invention provides an effective method for controlling thrips and spider mites, which are important pests of fruits and vegetables. c. The present invention provides a method for mass propagation of E. coli.
[0005]
[Means for Solving the Problems]
The present inventors have proposed A.I. c. In order to efficiently multiply large amounts of A. in a short period of time, the mixing ratio of various feeds was set to set A. c. As a result of searching for a system in which A. increases by, for example, 10 to 300 times the initial value (initial Ac number), A.I. c. By setting the number, the number of prey insects, the temperature and the humidity in a specific range, and reducing the amount of the culture medium, it has been found that a remarkably efficient and high growth effect can be obtained, and the present invention has been completed. That is, the present invention relates to A.I. c. The method for growing A. c. A. characterized by feeding 10 to 500 acarid mites as feed to several of them and setting the temperature at 15 to 35 ° C. and the humidity at 80 to 99% RH. c. Is provided.
[0006]
Hereinafter, the present invention will be described in detail.
[0007]
A. Used as a natural enemy in the present invention c. Is a known species, and is commercially available, for example, from Applied Bionomic in Canada or KOPPERT in the Netherlands, and is readily available.
[0008]
Next, A. c. Examples of the acarid mites to be used as bait include, but are not limited to, the mites Acarina, the red mites, the red mites, the red mites, and the red mites.
[0009]
Next, in the propagation method of the present invention, c. The number of acarid mites to be fed to one animal is 10 to 500, preferably 20 to 100.
[0010]
In the growth method of the present invention, the temperature and humidity of the environment can be appropriately selected during the growth, and the temperature is usually in the range of 15 to 35 ° C. and the humidity is in the range of 80 to 99%. -30 ° C, humidity ranges from 90-96%.
[0011]
The propagation method of the present invention can be performed in various containers, and the shape of the container can be used without any particular limitation. For example, when a 500 ml column-shaped plastic bottle (wide mouth) is used, A.p. c. For example, 2000 to 5,000 acarid mites are fed as feed to 10 to 100 animals, and after a few weeks, 15,000 to 25,000 A. c. Can be obtained. Examples of the medium include vermiculite, rice husk, bran, dried yeast and the like.
[0012]
What is important in practicing the present invention is how many A.D. c. Is required to meet the condition of the consumer who wants to be supplied by what time. c. By selecting the number of heads (initial head count), temperature and humidity, the number of heads desired by the customer can be supplied at the desired time.
[0013]
【Example】
Hereinafter, the present invention will be described with reference to examples.
[0014]
Example 1
A total of 50 g of bran and dried yeast was put into a 500 ml plastic bottle, and the mixture was allowed to stand still at a temperature of 30 ° C. and a humidity of 95% RH. c. When 100 animals are added, 20,000 A.F. c. I was able to get.
[0015]
Example 2
A total of 50 g of vermiculite and dried yeast were placed in a 500 ml plastic bottle and allowed to stand at a temperature of 20 ° C. and a humidity of 95% RH. c. When 100 animals are added, 20,000 A.C. c. I was able to get.
[0016]
Example 3
A total of 50 g of rice husks and dried yeast were put into a 500 ml plastic bottle, and allowed to stand still at a temperature of 25 ° C. and a humidity of 95% RH. c. After 10 weeks, 2,000 A.F. c. I was able to get.
[0017]
【The invention's effect】
According to the present invention, c. Can be dramatically increased.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17687594A JP3547173B2 (en) | 1994-07-28 | 1994-07-28 | Mite propagation method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17687594A JP3547173B2 (en) | 1994-07-28 | 1994-07-28 | Mite propagation method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH0840814A JPH0840814A (en) | 1996-02-13 |
JP3547173B2 true JP3547173B2 (en) | 2004-07-28 |
Family
ID=16021312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17687594A Expired - Lifetime JP3547173B2 (en) | 1994-07-28 | 1994-07-28 | Mite propagation method |
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JP (1) | JP3547173B2 (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1316896C (en) * | 2002-02-24 | 2007-05-23 | 张艳璇 | Orange pest mite preventing and killing method using cucumber amblyseius as predatory mite |
ES2345491T3 (en) | 2004-12-31 | 2010-09-24 | Koppert B.V. | COMPOSITION OF ACAROS, USE OF THE SAME, METHOD TO DEVELOP A PHYTOSEIID PREDATORY ACARO, DEVELOPMENT SYSTEM TO CREATE SUCH PHYTOSEIID PREDATORY ACARO AND PROCEDURES FOR BIOLOGICAL CONTROL OF PESTS IN A CROP. |
KR20140060587A (en) | 2004-12-31 | 2014-05-20 | 코퍼트 비.브이. | Mite composition, use thereof, method for rearing the phytoseiid predatory mite amblyseius swirskii, rearing system for rearing said phytoseiid mite and methods for biological pest control on a crop |
EA022197B1 (en) | 2005-12-29 | 2015-11-30 | Копперт Б.В. | Composition for biological pest control on plants, use thereof, a container for rearing a phytoseiid predatory mite, and methods for rearing a phytoseiid predatory mite and for biological pest control on a crop |
JP5271363B2 (en) * | 2011-01-06 | 2013-08-21 | コッパート・ベスローテン・フェンノートシャップ | Tick composition, use thereof, method for breeding predatory mite Amblyseiusswirskii, breeding system for breeding the predatory mite mite, and biological pest control method for crops |
JP6397334B2 (en) * | 2012-01-04 | 2018-09-26 | コッパート・ベスローテン・フェンノートシャップ | Mite composition comprising a predatory mite and immobilized food in contact with a fungal inhibitor and methods and uses relating to the use of said composition |
FR2992148B1 (en) * | 2012-06-22 | 2019-12-27 | Centre International D'etudes Superieures En Sciences Agronomiques | COMPOSITION COMPRISING ARTHROPODS AND EGGS OF ASTIGMATES |
CN103583506B (en) * | 2013-09-29 | 2015-09-09 | 北京市植物保护站 | A kind of method utilizing Amblyseius persidolongispinosus to prevent and treat horticultural crop evil mite |
CN104472437A (en) * | 2014-11-20 | 2015-04-01 | 中国农业科学院柑桔研究所 | Indoor isolation feeder for spider mites |
CN111543399A (en) * | 2020-06-24 | 2020-08-18 | 潮州中天农业科技有限公司 | Simple breeding method of Amblyseius barkeri |
CN111543398A (en) * | 2020-06-24 | 2020-08-18 | 潮州中天农业科技有限公司 | Predatory mite and prey mite feeding method |
KR102516525B1 (en) * | 2020-08-31 | 2023-03-31 | 거창군 | Methods of mass rearing and storage about Megoura crassicauda Mordvilko for breeding predatory natural enemies. |
-
1994
- 1994-07-28 JP JP17687594A patent/JP3547173B2/en not_active Expired - Lifetime
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JPH0840814A (en) | 1996-02-13 |
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