JP3494954B2 - Cosmetic composition, and its pack and bath agent - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to Perilla frutescens Britton var. AcutaKudo which is a Labiatae family plant.
And the proteolytic enzyme papain in combination in the formulation belong to the technical field of cosmetic compositions.

[0002]

2. Description of the Related Art Makeup is performed by women after puberty equally, and there are various methods for maintaining beautiful skin, such as prevention and improvement of fine lines and prevention and improvement of spots. Being developed. In cosmetics, as in the case of pharmaceuticals, it is essential to identify the cause of body troubles that should be prevented or improved, and prevent the cause of the troubles and improve the trouble state by blending active ingredients. A cosmetic product that complies with the above is prepared, but it is also important to prevent fine wrinkles and spots from occurring on the skin. However, in the case of fine wrinkles and age spots, the causes of fine wrinkles and age spots that occur at a relatively early age other than the common cause of aging are not known, and the causes of acceleration other than UV exposure have not been identified. Cosmetics based on such an unstable formulation base cannot bring good results, and the pursuit of beautiful skin using conventional cosmetics unnecessarily raises anxiety and dissatisfaction of women of makeup age. Needless to say small wrinkles, it is due to the imbalance and hyperkeratosis of the keratinized keratin solution of the skin epidermis, but this is the result, although there are various causes, it is not possible to determine, and there is no definitive method for prevention or improvement. .

Conventionally, it has been known to internally or apply a retinoid-related substance in order to prevent fine wrinkles and spots. In addition, with regard to spots, the cause is not known more than fine wrinkles, and the effect of prevention and improvement cannot be expected in the conventional cosmetics, but since the spots are the diffusion of melanin pigment in the skin, it can be applied to cosmetics. It is also known to blend a raw material having the ability to suppress the tyrosinase enzyme involved in the formation of melanin pigment.

A cosmetic containing papain is disclosed in
-182415, JP 10-287528, JP 10-
No. 203994 can be presented, but these are
Papain is mixed with a large amount of purified water, and papain is hydrolyzed by contact with water, or is subjected to a temperature rising operation, and the protein degradability is lowered, which is different from the present invention as described below. This is Papain.

[0005]

However, although oral administration or application of the above-mentioned retinoid-related substances has some effects, these substances affect the differentiation of cells and are not preferred, including skin thinning. Side effects are accumulated and cannot be used regularly. That is, the proteolytic enzyme used as a symptomatic means for accelerating keratinization is easy to use because it brings about smoothness of the skin by the decomposition of the protein on the surface of the epidermis and a smooth tactile sensation after use. However, there has been a problem that beyond the physical removal of the epidermis to be disentangled, necessary proteins of the epidermis are also removed, which may lead to a decrease in defense ability against external stimuli and a decrease in immune function. In addition, cosmetics can be used to improve stains by continuing the use of a raw material that has the ability to suppress the tyrosinase enzyme involved in the formation of melanin pigment, that is, a raw material that has been confirmed to have anti-tyrosinase activity. However, there is a problem in that the remarkable effects such as reduction and prevention of enlargement of stains are not obtained. In general, age and site of appearance of spots vary from person to person, and the background of appearance of spots involves complicated factors inside and outside the body.Therefore, according to the greatest common divisor of the prior art, the frequency of melanin pigment accumulated in the epidermis is increased. There is no choice but to stop physical removal once.

The present invention has been made in view of the above-mentioned problems, and it is possible to efficiently remove keratinized parts of skin which are delayed in keratinization and to remove excessively necessary proteins of the epidermis by one prescription,
In addition, it is another object of the present invention to provide a cosmetic composition that exhibits anti-tyrosinase active substance's melanin pigment diffusion-preventing effect and can be expected to have beautiful skin effects without side effects such as reduced immune function.

In order to solve the above problems, the invention of claim 1 provides a mixture in which an extract of Lamiaceae perilla is uniformly mixed with a powder or oily liquid containing substantially no water. After harvesting , the amount of papaya fruits that are not ripe
The lactate latex is refined to obtain a tamper of 40,000 Pa.UN / g or more.
And papain having an activity of click decomposition, the Labiatae Perilla
0.1 to the mixture in the state of the extract before the drying treatment.
~ 5.0 % by weight, and the papain to the final product
It was mixed with 0.1-3.0 wt% Te is a cosmetic composition, characterized in. According to another aspect of the invention, after drying the mixture obtained by mixing the powder or oily liquid containing no extract substantially moisture Shisoka perilla uniformly, secretions taken from ripe before fruit papaya The latex is purified to 40,000 Pa.
Papain with a proteolytic active amount of UN / g or more
Then, in the state of the extract before the drying treatment of the Lamiaceae
0.1 to 5.0 % by weight with respect to the mixture , and the papain
Is a cosmetic composition containing 0.1 to 3.0 % by weight of the final product . The invention according to claim 3 is a mixture obtained by uniformly mixing a perilla extract and a powder or an oily liquid containing substantially no water .
After being dried, the secretory latex obtained from the fruit of the papaya before ripening is purified into papain having a proteolytic activity amount of 40,000 Pa.UN / g or more, and the above Lamiaceae
Soybeans in the state of the extract before the drying treatment is 0.
1 to 5.0 % by weight and the above papain to the final product
It consists of a cosmetic composition obtained by mixing 0.1 to 3.0 wt% Te is a bath agent characterized.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The present inventor obtains cosmetics containing papain, which is a proteolytic enzyme that efficiently removes keratinized parts with delayed keratolysis, but does not excessively remove necessary proteins of the epidermis, In addition, the inventors have intensively studied and studied the combined use of an anti-tyrosinase active substance with a component capable of expressing the melanin pigment diffusion preventing effect, but a cosmetic composition that requires a combination of papain and a perilla extract can be expected to have a beautiful skin effect. The present invention was devised by experimentally finding out that. This cosmetic composition removes hyperkeratinized epidermis, which is a small wrinkle phenomenon by papain, and improves the action efficiency of perilla extract by the removal, and can prevent and improve spots by one prescription. .

A preferred embodiment of the present invention will be described below. Papain used in this embodiment is a papaya (Carica pa).
paya L.) secreted latex taken from unripe fruit,
It is lyophilized by performing operations such as primary centrifugation (5000 rpm), filter press filtration, secondary centrifugation (10000 rpm), sterilization filtration, etc. It is a pure product containing only papain ingredients (almost 100% by weight) and does not contain other ingredients.Based on the quantitative method listed in the "Papain", the compositional ingredient standard for each cosmetic variety, the active amount is 40,000 Pa.UN / g or more. It had an active amount. The degree of drying of the papain was determined according to the "Determination of Drying Weight by General Cosmetics Standard Test Method 8. Drying Weight Loss Test Method". About 1 g of the papain of this example was precisely weighed for 1 hour at 105 ° C. When dried, the weight loss was less than 10.0% of the collected amount.

The perillas used in this example are perilla frutescens Britton var.acuta Kudo or the related plants (Labiatae) leaves and branch tips. The perilla extract used in the present invention is obtained by extracting these with a solvent,
Although the perilla extract used in this example conforms to the standard for the composition of each cosmetic variety, the perilla extract used in this example was prepared by immersing 50 kg of perilla leaves in 500 l of 80% diluted ethanol and heating to 40 ° C.
Dissolved and extracted perilla components by standing for 48 hours and concentrated to 70% and filtered. Perilla components are dissolved in water and ethanol, but perilla extract is dissolved in water and ethanol. Ingredients are perillaldehyde, tannin,
It contained d-limonene and rosmarinic acid. Especially, rosmarinic acid, which is said to have a whitening effect, was 2.1 mg / ml.

As the action of perilla extract, anti-tyrosinase action, anti-inflammatory action, antibacterial action, anti-allergic action and the like are known, and all of them are preferable for maintaining beautiful skin, and if they are limited to the anti-tyrosinase action. , The activity of perilla extract is not necessarily high, it is about 47% of the apricot kernel extract of the same herbal medicine extract, which is about the same as the extract of Kumami and Clove, only 65% of the peach kernel extract, but with papain. It was found that the combination improves the action efficiency of the perilla extract, and that the anti-tyrosinase action can be utilized together with the known action, and the use of this as a cosmetic is the essence of the present invention. Incidentally, the base material of the cosmetic material applied to the present invention, before and after formulation, as long as there is no decrease in the protein degradability of papain, regardless of the dosage form, the amount of papain is 0.1 in the final product state. If it is less than weight%,
The effect cannot be expected, and when the amount is 3% by weight or more, the reaction of papain is excessive and the burden on the skin becomes large. Therefore, the amount of papain is preferably about 0.1 to 3.0% by weight in the final product. In addition, the perilla extract in the state of the extract before the drying treatment described below cannot be expected to have the above-mentioned actions and effects at 0.1% by weight or less, and due to a slight residual amount of the solvent contained in the perilla extract at 5.0% by weight or more. 0.1 to 5.0% by weight is preferable because it causes an irritating odor and decreases the protein degradability of papain.

Examples of the case where the cosmetic composition of the present invention is used in a pack are shown below. (1) Ingredient Ratio and Manufacturing Method The ingredient ratios of Examples 1 to 3 and Comparative Example 1 are as follows, and all conform to the standards and criteria stipulated in the cosmetic variety-specific permission criteria. [Example 1] Talc: 30.0 (same as below by weight) Glycerin: 8.0 Olive oil: 2.5 Polyoxyethylene sorbitan monolaurate (EO40) 1.0 Perilla extract (liquid) 1.0 Kaolin Remaining total 100.0 Mix the above components uniformly, remove excess water, and perform drying treatment so that water is not substantially contained, and then cool. In Example 1, 98% by weight of the mixture, which had been dried and allowed to cool, and slightly reduced, was uniformly mixed with 2.0% by weight of papain. This is because the perilla extract is basically dissolved in water and ethanol, but water and ethanol reduce the protein degradability of papain, so the mixture containing perilla extract is dried before mixing with papain. Then, by mixing with papain after making water and ethanol substantially not contained, the deterioration of protein degradability of papain was prevented. However, it is unavoidable that components other than papain absorb some amount of water in the air, but this is to prevent papain from coming into contact with water as much as possible. When the mixture was dried at 60 ° C. for 1 hour, the weight loss was 3.
It was 0% or less.

[Example 2] Talc: 30.0 (same as below by weight) Glycerin: 8.0 Olive oil: 2.5 Polyoxyethylene sorbitan monolaurate (EO40) 1.0 Perilla extract (liquid) ... 0.7 Kaolin Remaining total 100.0 In Example 2, 99% by weight of the mixture obtained by drying the above components in the same manner as in Example 1 was uniformly mixed with 1.0% by weight of papain.

[Example 3] Talc: 30.0 (same as below by weight) Glycerin: 8.0 Olive oil: 2.5 Polyoxyethylene sorbitan monolaurate (EO40) 1.0 Perilla extract (liquid) ... 0.7 Kaolin Remaining total 100.0 Example 3 is a mixture of 99.9% by weight of a mixture obtained by drying the above components in the same manner as in Example 1 and 0.1% by weight of papain.

[Comparative Example 1] Talc: 30.0 (same as below by weight) Glycerin: 8.0 Olive oil: 2.5 Polyoxyethylene sorbitan monolaurate (EO40) 1.0 Perilla extract (liquid) ... 0.7 Kaolin Remaining total 100.0 In Comparative Example 1, the above components were dried in the same manner as in Example 1, and papain was not mixed.

(2) Test method Subjects were 12 women (ages 33 to 46) who had pigment spots on the face and were aware of hot flashes when facial massage was performed. It was divided into A group, B group, C group, and D group. Group A is the pack of Example 1, Group B is the pack of Example 2, C
Group is the pack of Example 3 and Group D is Comparative Example 1
Each of the pack agents was used. The pack was kneaded with an equal amount of lukewarm water before use and applied to the face for 5 minutes, and was applied once every morning for a period of 12 weeks. In the meantime, the use of other skin-care cosmetics, vitamins A, C, E, and female hormones were prohibited. The four groups were examined for changes in the acid-alkali neutralization ability of the skin, which is a barometer for maintaining skin health (beautiful skin), and the degree of improvement in the lightness of pigmented skin. In addition, a questionnaire survey was conducted on the feeling of hot flashes felt during facial massage. Specifically, deterioration of the acid-alkali neutralization ability of the skin, which is one phenomenon of reduced skin function, is caused by an acidic state (pH 3.
It can be judged by the delay in the time required to recover from 0) and the alkaline state (pH 8.0) to the original state. Therefore, before and after this test, the subject's cheek was used as the measurement site, and the change in color was observed using a pH indicator that changes color due to changes in the pH value, and the required time was measured. That is, the action / effect can be estimated by how much the required time is shortened.

The test solution was a McKilvein buffer solution.
pH3.0 and pH8.0 were prepared, and as an indicator, bromphenol blue was used for pH3.0 and bromthymol blue was used for pH8.0. Bromphenol blue turns yellow at pH 3.0, but turns blue at pH 4.6, and bromthymol blue is p
It turns blue at H8.0, but turns yellow at pH 6.0. The results are shown in Table 1 of FIG. 1 and FIG. 2 which is a graph of Table 1. Also,
The lightness of the skin of the pigment spot was compared by measuring the cheek region previously determined for each subject with a colorimeter (Minolta CR-300). The measurement was performed 3 times, and the average value for each group was used. The results are shown in Table 2 of FIG. The unit is a unitless comparative numerical value where black is 0 and white is 100, and the larger the numerical value, the closer to white.
Furthermore, regarding changes in hot flashes felt during facial massage, a questionnaire was given to the subjects, and the results are shown in Table 3 in FIG.

(3) Test Results and Discussion First, in Table 1 of FIG. 1, the time required for acid neutralization is 0.4 minutes in Comparative Example 1, which is almost the same as when the pack agent is not applied. The required time is surely shortened as in the third and second embodiments, and is shortened by one minute in the first embodiment. Similarly, the time required for alkali neutralization is 0.3 minutes in Comparative Example 1, which is almost the same as when the pack agent is not applied, whereas the time required for Example 3 and Example 2 is definitely shortened, and In Example 1, it is significantly shortened to 2.4 minutes. Next, as shown in Table 2 of FIG. 3, the lightness of the skin is also measured by a color difference meter (Minolta CR-
Although the result measured by (300) does not change in Comparative Example 1,
It can be seen that the use of Examples 1 to 3 clearly improves the lightness by using each Example as compared with Comparative Example 1. Further, as shown in Table 3 of FIG. 4, the hot flash feeling of the skin was not changed in Comparative Example 1, but there was almost no hot flash feeling after use in each Example, and a slight hot flash feeling was observed in the use of Example 3. There was only one subject who had the.

Next, examples in which the following cosmetic composition of the present invention is used as a bath agent will be explained. (1) Ingredient Ratio and Manufacturing Method The ingredient ratios of Examples 4 and 5 and Comparative Example 2 are as follows, and all conform to the standards and criteria stipulated in the cosmetic variety-specific permission criteria. [Example 4] Perilla extract (liquid) ····· 2.0 (same as below by weight%) Sodium hydrogen carbonate Remaining total 100.0 The above components are uniformly mixed to remove excess water, and substantially no water is contained. After performing the drying process as described above, it is allowed to cool. In Example 4, 99% by weight of the mixture, which had been dried and allowed to cool, and slightly reduced, was uniformly mixed with 1.0% by weight of papain. This is similar to Examples 1 to 3,
The perilla extract basically contains water and ethanol,
Since water and ethanol decrease the protein degradability of papain, the mixture containing the perilla extract is dried before mixing with papain, and components other than papain may absorb some water in the air. Inevitable, but to prevent papain from touching water as much as possible,
By mixing with papain after making water and ethanol substantially not contained, it is possible to prevent the degradation of the protein degradability of papain. The degree of drying in the drying treatment was such that when the mixture of this example was dried at 60 ° C. for 1 hour, the reduction amount was 2.0% or less of the collected amount.

[Example 5] Perilla extract (liquid) ································ 2.0 Sodium hydrogencarbonate remaining total 100.0 In Example 5, 99.9% by weight of a mixture obtained by drying the above components in the same manner as in Example 4 was used. , 0.1% by weight of papain is uniformly mixed.

Comparative Example 2 Perilla extract (liquid) ···························· 2.0 Sodium bicarbonate remaining 100.0 Comparative Example 2 is the same as Example 4 except that the above components were dried. It is not mixed.

(2) Test Method Subjects were arbitrarily selected from 9 women (30 to 45 years old) who had pigment spots on the upper arm and were aware of hot flashes when facial massage was performed, and 3 women were divided into 3 groups. It was divided into E group, F group, and G group. The bath agent of Example 4 was used for the E group, the bath agent of Example 5 was used for the F group, and the bath agent of Comparative Example 2 was used for the G group. Immediately before bathing, the subject mixes 20 g of the bath agent with 180 to 200 liters of hot water in the bathtub, and
The bathing time was 18 minutes for more than a minute. In the meantime, the use of other skin-care cosmetics, vitamins A, C, E, and female hormones were prohibited. Skin health (Beautiful skin) for 3 groups
Changes in the acid-alkaline neutralizing ability of the skin, which is a barometer of maintenance, and the degree of improvement in the lightness of the pigmented skin were examined. In addition, a questionnaire survey was conducted on the feeling of hot flashes felt during facial massage. In order to investigate the deterioration of the acid-alkali neutralizing ability of the skin, the upper arm of the subject was used as the measurement site, and the test solution was prepared to have pH 3.0 and pH 8.0 using McKilvein buffer,
As an indicator, pH 3.0 is bromphenol blue, pH
Bromthymol blue was used for 8.0. Bromphenol blue turns yellow at pH 3.0 but turns blue at pH 4.6, and bromthymol blue turns blue at pH 8.0 but turns yellow at pH 6.0. Here, the deteriorated state of the acid-alkali neutralizing ability of the skin represents how the time required to recover from the acidic state (pH 3.0) and the alkaline state (pH 8.0) to the original state was shortened. The results are shown in Table 4 of FIG. 5, and FIG. 6 is a graph of Table 4.
In addition, the lightness of the skin of pigmented spots was compared by measuring the site of the upper arm that was previously determined for each subject by measuring with a colorimeter (Minolta CR-300) .Measurement was performed 3 times for each group. The average value for each is shown, and the test results are shown in Table 5 of FIG. Next, with respect to changes in the hot flashes felt when the facial massage was performed, a subject was surveyed and the results are shown in Table 6 in FIG.

(3) Test Results and Discussion First, in Table 4 of FIG. 5, the time required for acid neutralization is 0.4 minutes in Comparative Example 2, which is almost the same as when the bath agent is not used. The required time is surely shortened as in Examples 4 and 5, and in Example 4 it is shortened to 0.7 minutes. Similarly, the time required for alkali neutralization is 0.3 minutes in Comparative Example 2, which is almost the same as when the bath agent is not used, whereas the time required for Example 4 and Example 5 is certainly shortened. In Example 4, 1.5 minutes, which is somewhat inferior to the action of the pack agent, is certainly shortened. As shown in Table 5 of FIG. 7, the lightness of the skin was also measured by a color difference meter (Minolta CR-300) in Comparative Example 2, but there was no change, but the use of Examples 4 to 5 compared with Comparative Example 2. It can be seen that the brightness is clearly improved by the use of each example. Furthermore, as shown in Table 6 of FIG. 8, the hot flash feeling of the skin did not change in Comparative Example 2, but there was almost no hot flash feeling after use in each Example, and a slight hot flash feeling when using Example 5. 1 subject with
There were only people.

As described above, the cosmetic composition, the pack agent of the cosmetic composition, and the bath agent which require the combination of the perilla extract and papain of the embodiment of the present invention are papain which is a proteolytic enzyme. It was experimentally proved that, in addition to the skin-refining effect, the synergistic effect associated with the improvement of the action efficiency of perilla extract contributes to the health of the skin and contributes to the beautiful skin. Further, the test in the present invention is carried out by separately using the pack agent and the bath agent,
Although the action and effect were confirmed, it was confirmed by experiment that the pack agent and the bath agent can be used together, and the effect of the present invention can be more surely obtained by the combined use. Therefore,
Also in actual use, the combined use of multiple-dose type cosmetics is more effective.

It should be noted that the present invention is not limited to the above-described embodiment unless it impairs the features of the present invention.
It is needless to say that the cosmetic may be applied to a facial cleanser in addition to the pack agent and the bath agent, and the base material of the cosmetic may be any material as long as it does not substantially contain water. That is, as the bath agent, in the present example, the base material was only sodium hydrogencarbonate powder, but olive oil containing substantially no water may be added. Further, although the perilla extract of this example was a perilla family perilla, other perilla family perilla, for example, perilla may be used as long as it is a component equivalent to perilla.

[0026]

As described above, when the cosmetic composition of the present invention is used, in addition to the beautiful skin effect of papain, which is a proteolytic enzyme, and the synergistic effect of improving the action efficiency of perilla extract, the skin The effect of helping to improve the health of the skin and contributing to beautiful skin is obtained. In addition, one formulation effectively removes the keratinized part that delays the keratolysis of the skin, does not excessively remove the necessary protein of the epidermis, and in addition, expresses the melanin pigment diffusion-preventing effect of the anti-tyrosinase active substance, A skin beautifying effect without side effects such as a decrease in immune function can also be obtained.

[Brief description of drawings]

FIG. 1 is a graph in Table 1 showing changes in the acid-alkali neutralizing ability of skin by the pack of the present invention.

FIG. 2 is a graph of Table 1.

FIG. 3 is a diagram of Table 2 showing changes in skin lightness by a color difference meter using the pack agent of the present invention.

FIG. 4 is a diagram of Table 3 showing changes in “hot feeling” due to the pack agent of the present invention.

FIG. 5 is a chart in Table 4 showing changes in the acid-alkali neutralizing ability of skin by the bath agent of the present invention.

FIG. 6 is a graph of Table 4.

FIG. 7 is a diagram of Table 5 showing changes in skin lightness measured by a colorimeter using the bath agent of the present invention.

FIG. 8 is a chart of Table 6 showing changes in “hot feeling” due to the bath agent of the present invention.

Claims (3)

(57) [Claims] 1. A mixture of a Lamiaceae Lamiaceae extract and a powder or oily liquid containing substantially no water, which has been dried , and then dried, and then collected from unripe fruits of papaya. Minute
The lactate latex is refined to obtain a tamper of 40,000 Pa.UN / g or more.
And papain having an activity of click decomposition, the Labiatae Perilla
0.1 to the mixture in the state of the extract before the drying treatment.
~ 5.0 % by weight, and the papain to the final product
Cosmetic composition, characterized in that mixed with 0.1-3.0 wt% Te. 2. A mixture obtained by uniformly mixing an extract of Lamiaceae Lamiaceae and a powder or an oily liquid containing substantially no water, dried and then collected from unripe fruits of papaya. the secretions latex was purified with papain having an activity of 40000Pa.UN / g or more proteolytic, the Labiatae perilla
0.1 to the mixture in the state of the extract before the drying treatment.
~ 5.0 % by weight, and the papain to the final product
Pack agent characterized in that it consists of a cosmetic composition obtained by mixing 0.1 to 3.0 wt% Te. From wherein the Shisoka perilla extract substantially mixture was uniformly mixed was not a powder or oily liquid containing water <br/> drying process to, taken from ripe before fruit papaya the secretions latex was purified with papain having an activity of 40000Pa.UN / g or more proteolytic, the Labiatae perilla
0.1 to the mixture in the state of the extract before the drying treatment.
~ 5.0 % by weight, and the papain to the final product
Bath agent characterized by comprising the cosmetic composition obtained by mixing 0.1 to 3.0 wt% Te.