JP3487933B2 - Fluorescence observation device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [0001] BACKGROUND OF THE INVENTION 1. Field of the Invention
Irradiate the target site and observe the fluorescence image by the excitation light.
A fluorescent observation device. [0002] 2. Description of the Related Art In recent years, excitation light
Is irradiated, and the excitation light is directly generated from the living tissue.
Auto-fluorescence and the fluorescence of drugs injected into the body
It is detected as an image and the fluorescence image
Diagnose disease states (eg, disease type and invasion range)
Technology is being used to perform this fluorescence observation.
Have been developed. When a living tissue is irradiated with excitation light, the excitation
Fluorescence of a longer wavelength than light is generated. Fluorescent substance in living body
The quality is, for example, NADH (nicotinamide adenine)
Nucleotides), FMN (flavin mononucleotide)
C), pyridine nucleotides and the like. Recently, this
Between disease-causing endogenous substances that produce such fluorescence
Is becoming clearer, and the fluorescence of these is used to diagnose cancer and the like.
Is possible. As a fluorescent substance to be injected into a living body,
Is HpD (Hematoporphyrin), Photofrin, AL
A (δ-amino levulinic acid) and the like are used. this
These fluorescent agents have the ability to accumulate in cancer and the like.
The site of the disease can be diagnosed by injecting and observing the fluorescence.
In addition, a fluorescent substance is added to the monoclonal antibody,
There is also a method to accumulate fluorescent substance in the lesion by antibody reaction.
You. For example, laser light is used as excitation light.
Irradiating the living tissue with excitation light
Obtain a fluorescent image of the site. In the living tissue by this excitation light
A weak fluorescent light to generate a two-dimensional fluorescent image
Diagnosis and diagnosis. [0006] SUMMARY OF THE INVENTION Fluorescence observation as described above
Conventionally, the device is always constant from the light source for fluorescence observation
Of excitation light is emitted and irradiates the observation target site
It has become. For this reason, depending on the situation of the observation target site,
As a result, an appropriate amount of reflected light cannot be obtained, and
There is a possibility that a case where an observation image cannot be obtained may occur. [0007] The present invention has been made in view of these circumstances.
The amount of excitation light that is always appropriate for the observation target
To obtain the best observation image.
It is an object of the present invention to provide an efficient fluorescence observation device. [0008] Means for Solving the Problems The present inventionofFluorescence observation device
Irradiates excitation light to an observation target site of a living tissue to excite the excitation light.
In a fluorescence observation device that observes a fluorescent image caused by light emission,
A light source that generates the excitation light, and an observation target site of the excitation light.
Reflected light atFilter that removes the excitation light component
The fluorescent component of the reflected light through the filterLight receiving element to receive
And the amount of reflected light is detected based on the output of the light receiving element.
The amount of light emitted from the light source so that the output of the light receiving element becomes a predetermined amount.
Light quantity control means for adjusting theIt is characterized by the following. [0009] [0010] Embodiments of the present invention will be described below with reference to the drawings.
I do. 1 to 3 relate to a first embodiment of the present invention.
FIG. 1 is a configuration explanatory view showing a configuration of a main part of the fluorescence observation apparatus, and FIG.
2 shows the overall configuration of an example using an endoscope as a fluorescence observation device
FIG. 3 is an explanatory view showing the configuration, and FIG.
FIG. 4 is a characteristic diagram showing a spectrum of fluorescence. The fluorescence observation apparatus of this embodiment generates excitation light.
As a light source for fluorescence observation, for example, He-Cd (Helicopter)
Light source 1 having a laser light generating means.
Have. Configuration example using an endoscope as a fluorescence observation device
Is shown in FIG. The fluorescence observation device includes the light source 1 and the light source
1. A light source comprising:
A device 3 is provided. The light source device 3 is used for fluorescence observation.
Light guide cable 5 of endoscope 4 to be used is connected,
The incident end of the light guide 6 inserted into the cable is the light source 1
Are arranged at the light-emitting portion. The endoscope 4 transmits excitation light from the light source 1.
Light guide 6 and the fluorescent light
An image guide 7 for transmitting a light image is extended in the insertion portion.
It is composed. Excitation light is generated by the light guide 6
The light is transmitted to the distal end portion 8 of the endoscope insertion portion and illuminates the observation target site.
While the reflected light of the fluorescence
It is transmitted to the hand side by the image guide 7 and the observation target part
Fluorescent images can be obtained. FIG. 1 shows a configuration relating to the light quantity control of the light source 1.
You. In the vicinity of the exit end of the image guide 7, the excitation light component is
Filter to remove (for example, 442nm cut filter etc.)
A filter for removing the wavelength band of 350 to 500 nm) 9
A light receiving element 10 including a photodiode or the like is provided.
Light reflected by the light receiving element 10 at the observation target site.
Are received. Light receiving element 10
Is connected to the light quantity control means 2 and
Therefore, based on the output of the light receiving element 10,
The amount of reflected light is detected. Light intensity control means 2
Is connected to the light source 1 and the light amount control means 2
A control signal is sent to the light source 1 according to the amount of reflected light, and the light receiving element
The output light amount of the light source 1 is controlled so that the output of the light source 10 becomes a predetermined amount.
I will control it. FIG. 1 shows an example of a specific arrangement of the light receiving element and the like.
As shown in FIG. To the eyepiece 11 on the proximal side of the insertion section of the endoscope 4
Is provided with the output end of the image guide 7 and the image guide
In the optical path of the reflected light transmitted by the
A spectroscopic element 12 is provided, and through the spectroscopic element 12
The filter 9 and the light receiving portion are located at positions where the reflected light can be received.
An element 10 is provided. The filter 9 and the light receiving element
The child 10 may be provided in the endoscope operation unit. Light reception
The element 10 receives the signal inserted through the light guide cable 5.
Connected to the light amount control means 2 of the light source device 3 via
I have. The eyepiece 11 of the endoscope has a turtle for fluorescence observation.
Camera 13 is connected to the camera for fluorescence observation.
A fluorescence image of the observation target site is captured by the
Obtain images. Fluorescence observation camera 13
Image processing apparatus 1 for processing an image signal of a fluorescent image
4, the monitor 15 is connected in order, and the fluorescence image processing device 14
The fluorescence observation image obtained by signal processing in
It is displayed. The fluorescence observation camera 13 includes an objective lens 1
6. Filter 17 for passing fluorescent components, Filter 17
Intensifier that amplifies the image transmitted through
(I.I.) 18, output of image intensifier 18
A CCD 19 is provided as an imaging device for capturing an image,
The imaging signal output from the CCD 19 is sent to the fluorescence image processing device 14.
It is sent and signal-processed. Fluorescence image using the fluorescence observation apparatus of this embodiment
When observing and diagnosing light, first,
The laser light as the excitation light is applied to the light guide 6 of the endoscope 4.
And irradiates the portion to be observed (subject) 20 with the light. Do
And the irradiated laser light is reflected at the observation target site 20.
Fluorescence that has a longer wavelength than the laser light
And enters the image guide 7 of the endoscope 4. This reflected light
Exits the image guide 7 through the image guide 7
Emitted from the end and received through the spectral element 12 and the filter 9.
It is incident on the optical element 10 and fired through an eyepiece.
The light enters the light observation camera 13. The light receiving element 10 receives the reflected light of the fluorescent light
An output signal corresponding to the light amount is output, and the light amount control unit 2
The output signal of the light receiving element 10 is read to determine the amount of reflected light.
Detected, and the value of the reflected light amount becomes a predetermined value, for example, always constant.
Control signal to control the output (irradiation light) of the light source
Output to adjust the amount of light emitted from the light source 1. On the other hand, observation by the fluorescence observation camera 13 is performed.
A fluorescent image of the target site is captured, and the fluorescent image processing device 14
And processes the imaging signal to obtain a fluorescence observation image on the monitor 15.
To be displayed. At this time, as the filter 17, for example, λ
1 = 480-520 nm bandpass filter and .lambda.2 = 63
Using a bandpass filter of 0 nm or more,
The fluorescent images in each band by inserting
Image. Visible area at the observation target site by excitation light
As shown in FIG. 3, the spectrum of the fluorescence in the
Is λ0, the intensity distribution of the wavelength band longer than λ0 is
Strong at normal sites, especially around λ1, weak at lesions
Become. Therefore, especially from the fluorescence intensity around λ1,
It is possible to distinguish from a lesion, and such a fluorescent image
Thus, a lesion such as cancer can be diagnosed. In the fluorescence image processing device 14, for example,
From the image signals of the fluorescent images of λ1 and λ2,
Calculate the ratio or difference of the fluorescence intensity
Generate a fluorescent image that can determine the properties of the weave. In this embodiment, the light reflected from the portion to be observed
Read the amount and always check the
Set the light source so that reflected light with a constant light amount (constant light amount) is obtained.
The emitted light quantity is adjusted to a predetermined intensity. This allows you to
Always irradiate an appropriate amount of excitation light according to the target area
In the same good condition regardless of the observation site
Obtaining a fluorescent image by obtaining reflected light from the observation target site
A good fluorescence observation image so that the target diagnosis can be performed.
It is possible to obtain. FIG. 4 shows a fluorescence observation according to the second embodiment of the present invention.
FIG. 2 is a configuration explanatory diagram showing the entire configuration of the device. The second embodiment is based on the ordinary white illumination light.
Configuration of a fluorescence observation device that performs both endoscope observation and fluorescence observation
It is an example, and the light receiving element related to the light amount control is
Provided in the adapter inserted between the camera for fluorescence observation
Things. The eyepiece of the endoscope 4 has a normal endoscope observation.
Adapter that switches the optical path of the subject image for light and fluorescence observation
21 is attached, and for fluorescence observation through this adapter 21
The camera 13 is connected. Adapter 2
1 is a switching mirror 2 for switching an image obtained by the endoscope 4
2 is provided, and a normal endoscope is provided beside the switching mirror 22.
A CCD 23 for taking a mirror observation image is arranged. Further
Behind the switching mirror 22, the light is
A filter 9 and a light receiving element 10 are provided. Light receiving element 1
0 is inside the light source device 3 via a signal line inserted through the endoscope.
Output signal corresponding to the amount of reflected light.
No. is sent. The fluorescence observation camera 13 has a fluorescence observation
The image processing device for inspection 24 is a CCD 23 in the adapter 21.
Is connected to the normal observation image processing device 25, respectively.
The observation image generated by each image processing device is displayed on the display device 26.
It is displayed. When performing normal endoscope observation,
Illumination light is illuminated from the white light source to the observation target site and switched
By switching the mirror 22, the subject image obtained by the endoscope 4 is displayed.
The light is incident on the CCD 23 and imaged. And for normal observation
Normal observation by processing image signals in the image processing device 25
The image is displayed on the display device 26 as an image. On the other hand, when performing fluorescence observation, the light source 1
Excitation light is applied to the observation target site, and the switching mirror 22 is turned off.
Fluorescent observation of the fluorescent image of the subject obtained by the endoscope 4
The image is made incident on the camera 13. And fluorescence observation
The image signal is processed in the image processing device 24 for
It is displayed on the display device 26 as an observation image. At this time, observe
The light reflected from the target site is received by the light receiving element 10 and the first actual
In the same manner as in the embodiment, the light intensity of the light source 1
Control. As described above, the light receiving element related to the light quantity control is internal.
Not only inside the endoscope but also between the eyepiece and the fluorescence observation camera
It can also be provided on the adapter. Therefore, according to this embodiment,
If a new light-receiving element is installed in the eyepiece of the endoscope or the camera for fluorescence observation,
By attaching an adapter without attaching a child, white
Endoscope observation with color illumination light and fluorescence observation with excitation light
It is possible to switch and at this time the light receiving element
, The reflection of a predetermined amount of light is always performed as in the first embodiment.
You can adjust the amount of light emitted from the light source to obtain light.
A good fluorescence observation image can be obtained. FIG. 5 shows a fluorescence observation according to the third embodiment of the present invention.
FIG. 2 is a configuration explanatory diagram showing a configuration around a fluorescence observation camera of the apparatus.
is there. In the third embodiment, a light receiving element relating to light quantity control is used.
It is a configuration example provided in a fluorescence observation camera. A fluorescent observation camera connected to the eyepiece of the endoscope
In the camera 28, the spectroscopic element 12 behind the objective lens 16,
A filter 9 and a light receiving element 10 are provided, and a portion to be observed
It can receive the reflected light from. And
A filter 17 and an image intensity
A sifier 18 and a CCD 19 are provided to capture a fluorescent image.
It is possible. Light receiving element 10 in fluorescence observation camera 28
Is transmitted through the signal cable 29 extended from the camera.
Is connected to the light amount control means 2 in the light source device 3 and responds to the reflected light amount.
The same output signal is sent. In addition, light reception
The element 10 is provided with a fluorescent image processing device from the fluorescent observation camera 28.
The light amount control means of the light source device 3 is connected by a cable through the device 14.
It may be connected to the stage 2. As described above, the light receiving element for controlling the light amount is not
It can also be installed inside a light observation camera, in which case
However, as in the first embodiment, a predetermined amount of reflected light is always obtained.
Light intensity of the light source can be adjusted so that
A fluorescent observation image can be obtained. By the way, a fluorescence observation apparatus using an endoscope is required.
A camera for fluorescence observation on the eyepiece of the endoscope.
It is common to attach a fluorescent image. like this
The fluorescent observation camera connected to the endoscope is shown in FIG.
As described above, the objective optical system 52 passes through the fluorescent component in the camera 51.
Filter 53 and a CCD 55 as an image sensor
In addition, the fluorescence at the observation target site due to the excitation light is weak
Image is used as a means for amplifying the fluorescent image.
Intensifier (I.I.) 54 is arranged and configured
I have. As the image intensifier 54,
For example, cascade type II is generally used
However, such image intensifiers are large
Therefore, it is necessary to provide the image intensifier 54.
As a result, the fluorescence observation camera 51 becomes large and heavy.
Had become. For this reason, the fluorescence observation camera 51
When the camera is attached to the eyepiece 11 of the endoscope,
It can be grasped and operated by hand like usual endoscope observation
Difficult and requires support means to support the camera
I was That is, a fluorescence observation camera as shown in FIG.
A supporting member 56 such as an arm for supporting the portion 51
The main unit 57 or ceiling (not shown) or a bed for diagnosis
Attached to a part of the
It is possible to reduce the burden on the operator by supporting the la part
Had been. However, due to the support member, compared to the endoscope alone
Operability is inferior. In the following, the fluorescent image amplifying means is reduced in size and the fluorescent light is amplified.
Configuration of a fluorescence observation device capable of realizing a compact observation camera
Here is an example. FIGS. 7 and 8 show a compact fluorescent image amplifying means.
FIG. 7 relates to a first example of the configuration of a fluorescence observation apparatus,
FIG. 8 is a configuration explanatory view showing a schematic configuration around the inspection camera, and FIG.
Describes the schematic configuration and operation of the MCP as an optical image amplification means
It is an operation explanatory view for clarifying. When performing the fluorescence observation, the eyepiece 11 of the endoscope 4
Is connected to the objective lens 1
6. Filter 17 for passing fluorescent components, Filter 17
MCP (Micr) as amplifying means to amplify the image transmitted through
o Channel Plate, such as proximity focusing MCP-I.I.
32, CCD for capturing the output image of MCP32
19, and converts the imaging signal into an image for fluorescence observation.
The data is output to the image processing device 24. Viewing through the image guide of the endoscope 4
The reflected light from the target site is attached to the eyepiece 11
Through the objective lens 16 of the fluorescence observation camera 31
The excitation light component is removed by the filter 17 and the MCP 32
Is incident on. The MCP 32 is, as shown in FIG.
It has channels with a number of pores,
And a fluorescent screen 34 are provided. Incident on MCP32
The emitted light passes through the photocathode 33, and each channel of the MCP
Electrons are generated in the channel, and a predetermined voltage 35 is applied to the electrodes on both sides.
The light is amplified by the addition and emitted through the fluorescent screen 34.
You. Here, the light of the fluorescent image incident on the MCP 32 is 10
Amplified from 00 to 10000 times and incident on CCD 19
It is. With this MCP 32, the fluorescence of a weak observation target site is
The light image is amplified to a visible light image, and the CCD 19
Image. MCP32 is a general image intent.
Much smaller and cascaded I.I.
It has a luminous flux image magnification comparable to that of
Light of a desired intensity can be obtained from weak fluorescence. In the CCD 19, the fluorescent image is converted into an electric signal.
Is converted to an image processing signal for fluorescence observation 24 as an imaging signal.
Output, signal processed by image processing device and fluorescent to monitor
It is output as an observation image. Thus, the image is used as a means for amplifying the fluorescent image.
MCP in front of CCD instead of page intensifier
, The size of the fluorescent image amplification means can be reduced.
The fluorescence observation camera can be made smaller and lighter,
When the fluorescence observation camera is attached to the eyepiece of the endoscope,
Cameras for fluorescence observation and
It is possible to operate with the operation unit. Therefore, fireflies
Operability during light observation can be improved. FIG. 9 shows fluorescent light obtained by miniaturizing the fluorescent image amplifying means.
FIG. 9 is a schematic configuration diagram of the entire apparatus according to a second configuration example of the observation apparatus.
is there. In this embodiment, an electric power supply having a CCD at the tip of the insertion portion is used.
This is an example of an apparatus using the child endoscope 36, and the insertion of the endoscope 36 is performed.
A filter is provided on the rear side of the objective optical system
17, MCP 32 and CCD 19 are provided. this
The observation device 39 to which the endoscope 36 is connected emits excitation light.
Light source 1 to be generated and an MCP driving unit 4 for driving the MCP 32
0 and a fluorescent image processing device 14 that performs signal processing of the fluorescent image
The excitation light from the light source 1 is
Incident on the guide 6 and the fluorescent light obtained by the endoscope 36.
Signal processing of light image and output to monitor 15 as image signal
Then, a fluorescence observation image is displayed. As described above, the endoscope is inserted into the distal end portion of the insertion portion of the endoscope.
Filters, MCPs, CCDs, etc. can be provided.
In this case, the endoscope should be used to obtain a fluorescent image of the desired brightness.
This makes it possible to reduce the size of the device and improve operability.
Can be up. FIG. 10 shows a fluorescent image obtained by miniaturizing the fluorescent image amplifying means.
FIG. 7 is a schematic configuration diagram of an endoscope according to a third configuration example of the light observation device.
is there. This example is a modification of the second configuration example,
This is an example in which a CCD is provided in the operation unit on the hand side of the mirror. Introspection
An emission end of the image guide 7 is provided in the operation unit 42 of the mirror 41.
Filter 17, MCP 32, CCD 19
ing. As described above, the fill in the operation section of the endoscope is performed.
Data, MCP, CCD, etc. can be provided.
The fluorescent image of the desired brightness can be obtained with the endoscope.
In this way, the device can be downsized and operability can be further improved.
Can be. FIG. 11 shows a fluorescent image obtained by miniaturizing the fluorescent image amplifying means.
As a fourth configuration example of the light observation device, fluorescence observation and normal white
1 shows a configuration of an apparatus for performing endoscope observation using colored light or the like. The fluorescence observation apparatus of this embodiment performs fluorescence observation.
For fluorescence observation of He-Cd laser etc. that generates excitation light
The light source 61 emits illumination light for performing normal endoscopic observation.
Normally having a light source lamp 62 such as a generated xenon lamp
And a light source 63 for observation.
Connected to the light source adapter 64 for switching the light from the light source
Have been. The light source adapter 64 is connected to the light sources 61 and 63
Selectively switch the light to guide the light to the endoscope
To change the angle between the switching mirror 65 and the switching mirror 65
And a driver 66 for driving.
Of the light guide 5 are connected. The eyepiece 11 of the endoscope 4 has a fluorescent observation
Camera adapter 6 for switching the camera for normal observation
7 is attached, and through this camera adapter 67
Fluorescence observation camera 68 and normal observation camera 69 are connected
Have been. The camera adapter 67 is obtained from the endoscope 4.
Camera 68 for fluorescence observation or camera for normal observation
A switching mirror 70 for switching to lead to
And a driver 71 that drives to change the angle of the
Have. The fluorescent observation camera 68 is driven by a motor 72.
73 that passes through the fluorescent component that is rotationally driven
And an MCP 32 for amplifying the fluorescent image, and capturing the fluorescent image
Observation target part provided with endoscope 4 equipped with CCD74
Are picked up. The filter 7
3 cuts the excitation light of the fluorescence observation light source 61 and
At least one type that passes light in a band with a longer wavelength
, For example, the above-mentioned λ1 and λ2
Two filters are arranged to pass the light of
Formed by the motor 72 and rotated by the motor 72.
The light passes through the next two bands. The normal observation camera 69 has a CCD 75.
The illumination light such as a normal white light obtained by the endoscope 4.
Images of the observation target part (normal observation images) are now taken
ing. The fluorescence observation camera 68 has a fluorescence observation device 7
6 is connected so that the image pickup signal of the fluorescent image is processed.
It has become. The fluorescence observation device 76 has an output of the CCD 74.
Image processing of the force imaging signal as described above
A fluorescent image processing unit 77 for generating a signal and a motor 72 are driven.
Timing to control the timing of the filter 73 by dynamic control
And a switching controller 78. The camera 69 for normal observation has a camera control
Is connected to the CCU 79
Therefore, the image pickup signal output from the CCD 75 is subjected to signal processing and transmitted.
A normal observation image signal is generated. The fluorescence observation device 76 and the CCU 79 are
The fluorescence observation image signal is connected to the observation image control device 80.
The normal observation image signal is input separately.
You. The observation image control device 80 includes a light source adapter 64 and
Drive control of drivers 66 and 71 of camera adapter 67
To control the switching timing of the switching mirrors 65 and 70
Timing controller 81 and a timing controller
Fluorescence in synchronization with adapter switching control by controller 81
Video switching between observation image signal and normal observation image signal
And a switching circuit 82. To the observation image control device 80
Is connected to the foot switch 83, and this foot switch
83 and an image based on the image switching instruction from
The image signal can be switched. The image output terminal of the observation image control device 80
Is connected by the video switching circuit 82
The fluorescence observation image signal or the normal observation image signal
The fluorescence observation image or the normal observation image is input to the
It is displayed. When observing with the fluorescence observation apparatus of this embodiment
Command is issued by the foot switch 83 to switch the image.
And turn off the light source and camera with the adapters 64 and 67.
Alternatively, select fluorescence observation or normal observation. When performing fluorescence observation, the adapters 64 and 6
7, a fluorescence observation light source 61 and a fluorescence observation camera 6
Switch the optical path to the side 8 and take a fluorescent image, and then use a fluorescence observation device
Obtain the fluorescence observation image signal from 76 and observe the fluorescence on the monitor 84
Display an image. On the other hand, when viewing a normal endoscopic image,
In this case, the normal observation light source 63 is
And switching the optical path to the side of the camera 69 for normal observation,
The endoscope image is captured by the illumination light, and is normally viewed from the CCU 79.
Obtain an observation image signal and display a normal observation image on the monitor 84
You. With such a configuration, fluorescence observation and ordinary
Endoscope observation using white light or the like can be performed. This example
Fluorescence observation that performs fluorescence observation and normal endoscope observation like
In the system, the fluorescence image is increased by the fluorescence observation camera.
M instead of image intensifier as width means
By arranging the CP, the size and weight of the camera can be reduced.
Endoscope operation unit including adapter and camera
The surrounding area can be made smaller, and the operability during fluorescence observation can be improved.
Can be [Appendix] As described in detail above, the present invention
According to the aspect, the following configuration can be obtained.
That is, (1) Before irradiating the excitation light to the observation site of the living tissue
In a fluorescence observation device that observes a fluorescence image with the excitation light
A light source for generating the excitation light;
A light receiving element for receiving the reflected light at the elephant site;
The amount of reflected light is detected based on the output of the element, and the output of the
Light amount for adjusting the amount of light emitted from the light source so that is a predetermined amount
And a control unit. (2) The fluorescence observing device is provided with the excitation light
Guide transmitting the light to the tip end of the insertion section and the fluorescent image
Image guide to transmit the image to the eyepiece at hand
An endoscope for connecting the light receiving element to the endoscope.
The fluorescent light according to claim 1, wherein the fluorescent light is arranged in the eye.
Observation device. (3) The fluorescence observing device is provided with the excitation light
Observation turtle that captures a fluorescence image of the observation target site
Camera for the fluorescence observation
The fluorescence observation described in Supplementary Note 1, wherein the fluorescence observation is provided in
apparatus. (4) The fluorescence observing device is provided with the excitation light
Guide transmitting the light to the tip end of the insertion section and the fluorescent image
Image guide to transmit the image to the eyepiece at hand
Endoscope, and a fluorescence image of the observation target site by the excitation light.
A fluorescence observation camera for imaging, and an eyepiece and a front of the endoscope.
The endoscope is inserted between the fluorescent observation camera and the endoscope.
Fluorescence image by excitation light on the exit optical path of the image transmitted to the eye
And an adapter for switching between an optical image using white illumination light and
And the light receiving element is disposed in the adapter.
3. The fluorescence observation device according to claim 1, wherein: (5) Incident light is introduced into the optical path of the fluorescent image.
The splitting element is divided into two
Characterized in that the light receiving element is arranged behind the emitting surface.
2. The fluorescence observation device according to claim 1. (6) Excitation light is applied to the observation target site of the living tissue
Observation to observe the fluorescence image by the excitation light
A light source for generating the excitation light;
Imaging means for capturing a fluorescent image of a site to be observed by light;
MCP (microchip) as a means for amplifying the fluorescent image
An image amplifying element comprising a channel plate).
And a fluorescence observation device. (7) The imaging means includes a solid-state imaging device.
The solid-state image sensor has an image incident surface in front of it.
In addition 6, the image amplifying element is provided.
The fluorescence observation device according to the above. [0071] As described above, according to the present invention,
Always irradiate an appropriate amount of excitation light according to the target site
The optimal observation image can be obtained.
There is fruit.

BRIEF DESCRIPTION OF THE DRAWINGS FIGS. 1 to 3 relate to a first embodiment of the present invention,
FIG. 1 is a structural explanatory view showing a configuration of a main part of a fluorescence observation apparatus. FIG. 2 is a structural explanatory view showing an overall configuration of an example using an endoscope as a fluorescent observation apparatus. FIG. FIG. 4 is a characteristic diagram showing a spectrum of fluorescence. FIG. 4 is a configuration explanatory view showing an entire configuration of a fluorescence observation device according to a second embodiment of the present invention. FIG. 5 is a fluorescence observation of a fluorescence observation device according to a third embodiment of the present invention. FIG. 6 is an explanatory diagram showing a configuration example of a fluorescence observation device using an endoscope. FIG. 7 and FIG. 8 are miniaturized means for amplifying a fluorescent image. 7 relates to a first configuration example of a fluorescence observation apparatus, and FIG. 7 is a configuration explanatory view showing a schematic configuration around a camera for fluorescence observation. FIG. 8 is a diagram for explaining a schematic configuration and operation of an MCP as a fluorescence image amplification unit. [FIG. 9] A fluorescence observation apparatus in which the fluorescent image amplification means is miniaturized. FIG. 10 is a schematic configuration diagram of an entire apparatus according to a second configuration example. FIG. 10 is a schematic configuration diagram of an endoscope according to a third configuration example of a fluorescence observation device in which a fluorescence image amplifying unit is downsized. As a fourth configuration example of the fluorescence observation apparatus in which the amplification means is downsized, a configuration explanatory view showing the configuration of an apparatus for performing fluorescence observation and endoscope observation using ordinary white light or the like [Explanation of symbols] 1. Light source 2 light amount control means 3 light source device 4 endoscope 6 light guide 7 image guide 9 filter 10 light receiving element 13 fluorescence observation camera 14 fluorescence image processing device 20 observation target site

Continued on the front page (72) Inventor Masaya Yoshihara 2-43-2 Hatagaya, Shibuya-ku, Tokyo O-limpus Optical Industry Co., Ltd. (72) Inventor Masahiko Iida 2-43-2 Hatagaya, Shibuya-ku, Tokyo O-limpus Optics (72) Inventor Yasuhiro Ueda 2-43-2 Hatagaya, Shibuya-ku, Tokyo Olympus Optical Co., Ltd. (56) References JP-A-57-89842 (JP, A) (58) Field (Int.Cl. ⁷ , DB name) A61B 1/00-1/32 G01N 21/64

Claims (1)

(57) [Claim 1] In a fluorescence observation apparatus which irradiates excitation light to an observation target part of a living tissue and observes a fluorescent image by the excitation light, a light source which generates the excitation light; Excitation light component of reflected light at the observation target site of the excitation light
, A light-receiving element for receiving the fluorescent component of the reflected light via the filter, and a light source for detecting the amount of reflected light based on the output of the light-receiving element, so that the output of the light-receiving element becomes a predetermined amount. And a light amount control unit that adjusts the amount of emitted light of the fluorescence observation apparatus.