JP3430940B2 - Foods and beverages combining isoflavones and peptides - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to foods and beverages in which isoflavones and peptides are combined.

[0002]

2. Description of the Related Art Osteoporosis has become a problem as a disease caused by a decrease in female hormone (estrogen) after menopause resulting in Ca (calcium. Osteoporosis is a kind of bone disease in which the bone density decreases due to the outflow of Ca. When suffering from this, a bone fracture is likely to occur even with a light impact. Elderly women have a particularly slow recovery,
Once a bone fracture occurs, in the worst case it can be bedridden. So recently, C which is the main component of bone
A functional material having an effect of promoting absorption of a or suppressing Ca outflow from bone has been developed. Of these, soy isoflavone, which is said to act like a female hormone in the body and prevents the loss of bone mass due to hormone loss in postmenopausal women, has been receiving particular attention.

In accordance with such trends, the number of foods and drinks that claim to strengthen bones is increasing. As an example, 1. Focusing on Ca, which is the main component of bone, and adding only Ca material 2. 1. A material that promotes the absorption of Ca (vitamin D, casein phosphopeptide, etc.) Items added to 3. 1. A material that suppresses the outflow of Ca from bone (soy isoflavone, etc.) Or 2. Added to 4. In order to balance the mineral balance related to Ca, 1. Through 3. Items added to 5. Vitamin K, which is said to promote bone formation, 1.
Through 4. Product development can be seen from the perspective of any of the items added to.

The above-mentioned bone-reinforced foods all have the idea that bone mass is maintained by promoting absorption and outflow of Ca, which is the main component of bone, and strengthening the bone itself to prevent bone diseases such as osteoporosis and further fracture. Is based on. However, for the purpose of preventing osteoporosis and further bone fracture, the above bone-reinforced food is insufficient in the following points. That is, (1) strengthening the bone itself requires not only supplementation, absorption promotion, and outflow suppression of Ca, but also supplementation of another main component, protein. (2) In the case of elderly people, 80% of the direct causes of bone fractures are due to falls, and this is due to weakness of the muscles of the legs and waist. Therefore, not only strengthening the bone itself, but also preventing fracture
It is also necessary to strengthen the muscles that surround the bone. To meet the above two points, it is necessary to supplement protein. Furthermore, as a protein source, a peptide obtained by hydrolyzing a protein is more preferable because it does not impose a burden on digestion and absorption even in an elderly person and is rapidly absorbed into bone and muscle.
That is, the following 6th category which is not included in the above 5 categories / viewpoints.
There is a background in which the above items are also desired from the above points (1) and (2). 6. It would be desirable to have isoflavone peptides for strengthening the bone itself and for strengthening the muscle that wraps the bone with peptides or the like.

A large number of patent publications relating to isoflavones or peptides have been published. As a major prior application, International Publication WO95 / 16362 discloses that an isoflavone compound containing a large amount of aglycone is obtained by decomposing the glycoside of the isoflavone compound in beans by koji mold. Further, in Japanese Patent Laid-Open No. 10-114653,
A composition for promoting bone formation and preventing a decrease in bone mineral content, characterized in that isoflavone is the main active ingredient, useful for the treatment and prevention of osteoporosis, bone fracture, etc., and a composition that can be used in foods or pharmaceuticals is obtained. Singing thing. On the other hand, Japanese Patent Application Laid-Open No. 09-216834 claims to provide foods, drinks, medicines or materials to which kininogen and / or a degradation product of kininogen is added as an active ingredient to promote the action of bone formation or bone resorption inhibitor. . However, these conventional techniques are different from the above-mentioned foods and beverages containing isoflavone and peptides, and further the problem.

[0006]

[Problems to be Solved by the Invention] From the viewpoints and categories described above, it is an object to provide foods and drinks that can help strengthen bones and strengthen muscles that widely surround the bones with foods and drinks that contain isoflavones and peptides.

[0007]

According to the present invention, (1) isoflavone is added and isoflavone in dry weight is added.
Foods and drinks containing 0.4% or more of peptides
Fee, (2) isoflavone soybean hypocotyl, the dry heat heating products, walk
Is obtained from its water-soluble solvent extract or organic solvent extract.
(1) The food and beverage as described above, (3) Peptide isolated soybean protein with protease
The above (1) which is a hydrolyzed soybean peptide or
(2) Food and beverage, (4) Isoflavone used contains dry isoflavone.
1% by weight or more, the peptide content used is dry weight
The above protein content is 50% by weight or more
The food according to any one of (1) to (3), (5) Ca (calcium) -containing material, minerals, bitter
Promotes absorption of Ca or elution of Ca other than mines and isoflavones
Of the above (1) to (4), which contains a functional material having a suppressing effect
To provide the food and beverage according to any one of
It

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. The present invention relates to providing foods and beverages containing isoflavones and peptides.

First, the terms of the present invention will be explained. Any isoflavone may be used as long as it contains isoflavone and the like in which active ingredients such as isoflavones are not lost. Preferably, a high content of isoflavones and the like is preferable, and in particular, high content of soy hypocotyls and those whose flavor has been improved by heat treatment or isoflavones obtained by extracting them with an aqueous solvent such as water and hydrous alcohol are contained. An amount of processed soybean hypocotyl and the like is suitable. Isoflavone referred to in the present invention is the total amount of daidzin, genistin, daidzein, genistein, the quantitative determination of each component by reflux extraction of a certain amount of the sample and quantifying each component by high performance liquid chromatography, the total amount is calculated. To do. The peptide may be any plant or animal material as long as it contains a peptide that is a hydrolyzate of protein. It is preferable that the peptides have a high content, and a hydrolyzate of soybean protein is particularly preferable. In the quantification of the peptide referred to in the present invention, nitrogen is quantified by a known Kjeldahl method or a method similar thereto in a fixed amount of a test substance, and a nitrogen coefficient (6.25)
Calculate by multiplying by. Healthy foods are foods (all foods and drinks; however, pharmaceuticals and quasi-drugs are not included), supplemented with nutritional components, or sold as those suitable for special health uses. Says the food used for food.

Soybean-derived means soybean or a part thereof or classification, separation, pulverization, mixing, heating, extraction, hydrolysis, etc. thereof.
It refers to processing by selecting from refrigeration, freezing, drying, molding, etc., and combining. Soybean-derived isoflavones are, for example, Japanese Patent Publication No. 04-48417 (humidity control, heating, peeling, soybean hypocotyl collection), Japanese Patent Publication No. 05-33016 (alkaline solution immersion, roasting, soybean hypocotyl treatment method). , Special fair 07-
238089 (protein isolate containing a large amount of isoflavone compound and method for producing the same) and the like.
The peptide derived from soybean is, for example, JP-A-07-264.
No. 993 (Production method of soybean peptide mixture), JP-A-07-284369 (Peptide composition and production method thereof), JP-A-09-132592 (Peptide-bonded hydrolyzate production method and peptide bond used therefor) Water-decomposing agent), Japanese Patent Publication No. 09-507478 (manufacturing method of peptide product and obtained product). The dry weight is the weight% of the dry solid content by a general dry weight loss method (105 ° C. for 4 hours).

[0012] Isoflavone is a kind of phytoestrogen which exhibits the same action as estrogen (female hormone), and is said to prevent elution of calcium (Ca, hereinafter abbreviated) from bone. Soybean contains a large amount of isoflavone components, and exists in the form of glycosides such as daidzin, genistin, and gristin, aglycones from which sugars have been removed, and malonyl bodies in which a malonyl group is bound to glycosides. . Therefore, since soybean peptide also contains a very small amount of isoflavone, it is expected that the effect will be further enhanced by using this as a protein source. Japanese people take isoflavone 10 to 20 mg on average on an average daily basis, but it is said that it is desirable to additionally take 10 to 20 mg daily for the prevention of osteoporosis and the like.

[0012] Protein, like Ca, is the main component of bone, and since Ca strengthening alone is not sufficient to increase bone mass, it is also essential to take in protein at the same time. on the other hand,
Protein is a component of muscles, and in order to increase the amount of muscle that declines with age, protein intake is essential.

[0013] Peptides can be said to be processed proteins and have similarities to the effects of proteins, but are particularly excellent in the following points. That is, the absorption rate into the body is higher than that of normal proteins, and it is efficiently used for the synthesis of bone proteins and muscle proteins. Moreover, since the burden on the gastrointestinal tract due to digestion and absorption is small, it is very effective as a protein source that tends to be insufficient in elderly people and sick people. The daily requirement for protein is 60 g on average, but elderly people tend to have low intake of protein and have low digestion and absorption. Therefore, it can be expected that ingesting 5 g of a digestible peptide as a protein source further daily will be effective for muscle strengthening.

The present invention contains both isoflavones and peptides, and the isoflavone content of the dry weight of the peptides is
Foods and beverages with 0.2% or more. It is considered that ingestion of isoflavone and peptide at an appropriate ratio as described above will effectively supplement the shortage and help prevent bone fracture and the like in the elderly. The isoflavone content is preferably 0.2% or more and more preferably 0.4% or more by dry weight with respect to the peptide. Regarding the upper limit of 0.4% or more, it is necessary to set the% value of the isoflavone content to the peptide based on the types and intakes of foods and beverages that combine isoflavones and peptides, and the intake ratio in human daily nutritional requirements. Good.

The isoflavone-containing product of the present invention can be used, for example, from soybean hypocotyl and the like, and can also be used as a dry-heated product thereof, or a water-soluble solvent extract or organic solvent extract thereof, and their production methods are known. It is also possible to use The peptide-containing material of the present invention can use, for example, soybean peptide obtained by hydrolyzing isolated soybean protein with a protease,
The manufacturing method thereof may be a known method.

[0016]

EXAMPLES The effectiveness of the present invention will be shown below together with examples, but the technical idea of the present invention is not limited by these examples.

[0017]

Example 1 Effects of Bone Reinforcement and Muscle Reinforcement Using an ovariectomized model rat, the effect of suppressing bone loss caused by osteoporosis and the effect of muscle strengthening were examined by administration of food and drink containing peptide and isoflavone. . (Breeding method) Ovariectomized model rats (15 weeks old,
Pregnant weeks) were divided into a control group (C group) and an isoflavone peptide ingestion group (IP group), and 6 animals were grouped and raised for 4 weeks. Group C is a solid feed "MF" (water 7.9%, protein 23.3%, carbohydrates 57.7).
%, Manufactured by Oriental Yeast Co., Ltd. The same applies hereinafter) 2
0 g was orally administered daily. For the IP group, the same solid feed was treated with the peptide-containing and soybean-derived "High New-DC
5 "(water 6%, peptide 75%, isoflavone 0.0
7%, manufactured by Fuji Oil Co., Ltd. The same shall apply hereinafter) as a protein, and 3.5% by weight of soybean hypocotyl extract / isoflavone-containing material "Soyaflavone-E" (water content 5%, isoflavone content 4%; manufactured by Fuji Oil Co., Ltd .; hereinafter the same). Was added as isoflavone in an amount of 10 mg% by weight, and was orally administered daily in the same manner. Conditions other than feed are group C, IP
The group was the same. (Training Method and Measurement of Muscle Mass) In order to give a given exercise load per day to the rats, the rats were allowed to take a swimming exercise in a bathtub. The maximum swimming time was the time when the rat was unable to swim. After the breeding for 28 days, the weight of the gastrocnemius muscle (Hifukukin) and quadriceps muscle (Shitokin) of the rat was measured. (Measurement of Bone Density) The femur was extracted, its wet weight and the volume were measured with a pycnometer, and the bone density was calculated. The results of muscle weight measurement and bone density measurement are shown in Table 1.

[0018] Table 1 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−                     Group C IP group Significant difference (p) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Gastrocnemius 0.96 ± 0.02 1.07 ± 0.02 <0.05 Quadriceps 1.37 ± 0.03 1.46 ± 0.02 <0.05 Bone density (g / ml) 1.31 ± 0.03 1.48 ± 0.04 <0.05 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− When p <0.05, there was a significant difference at a risk rate of 5%.

From the results shown in Table 1, the isoflavone and peptide ingested groups had significantly higher bone density and muscle mass than the control group, and the effects of bone strengthening and muscle strengthening were recognized.

[0020]

[Example 2] Beverage preparation and muscle-enhancing effect A raw material was prepared according to the composition shown in Table 2 and subjected to UHT sterilization to prepare a beverage according to the present invention.

[0021] Table 2 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Beverage containing both peptide and isoflavone (200g) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Peptide inclusion 6.7 g Soybean hypocotyl extract 0.25g 16.0 g sugar Grapefruit juice concentrate 1.2 g Acidulant 1.0 g Calcium lactate 0.8 g Vitamin D3 17I U Grapefruit flavor 0.1 g Water 172.15g −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−                     200.0 g in total −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−

The beverage contains 0.3% isoflavones based on the peptide. By ingesting 200g of this beverage, soybean peptide as a protein can be supplemented with 5.0g and isoflavone with 15mg, and further 100mg of calcium (1/6 of Japanese daily nutritional requirement) and vitamin D17IU (Japanese). It was set to be able to supplement 1/6 of the daily nutritional requirement. The exam is a member of university sports club
There were 0 panelists, and this beverage administration group (P group) and a control group (C which omitted peptide and isoflavone from this beverage)
It was divided into two groups (5 groups each). Each group of panelists decided to incorporate weight training 3 days a week, and both groups P and C were allowed to drink each beverage twice a day (once after practice and before bedtime) for 3 months. The muscular strength was measured by using "Power Max V" (manufactured by Combi Co., Ltd.) to perform full-power rowing for 10 seconds twice with a load intensity of 0.05 times the weight of the panelist, and finding the maximum number of revolutions at that time. It was Table 3 shows the measurement results of the maximum rotation speed of each group up to 3 months.

[0023] Table 3 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−   Comparison of the amount of change from the start of the maximum rotation speed of each group −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−                     P group C group Significant difference (p) −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Start 0.0 0.0 − 1 month later 11.0 3.0 <0.1 3 months later 19.0 9.0 <0.1 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− When p <0.1, there is a significant difference at a risk rate of 10%.

As shown in Table 3, when the maximum rotational speeds of the respective groups were compared, the P group significantly increased as compared with the C group. From this, it was confirmed that the food and drink containing soybean peptide and soybean isoflavone also had a muscle enhancing effect in humans.

[0025]

Example 3 Jelly Containing Peptide and Isoflavone A raw material was prepared in the composition shown in Table 4 to prepare 20 g / piece of jelly. Table 4 Jelly formulation (100 g) containing both peptide and isoflavone --------------------------------------. ------------------ Peptide-containing product 6.7 g Soybean hypocotyl extract 0.25 g Sugar 15. 0 g Grapefruit concentrated fruit juice 10.0 g Gelling agent 1.2 g Liqueur 1.5 g Grapefruit flavor 0.2 g Water 65.15 g ------------------------------------ ------------- Total 100.0 g ---------------------------------------- By ingesting 5 of this jelly, the same amount of peptide and isoflavone as in the beverages shown in Table 2 can be ingested. This jelly can be applied to a health food for the purpose of nutritional supplementation.

[0026]

Example 4 Cookie Containing Peptide and Isoflavone The raw materials were prepared according to the composition shown in Table 5, and 10 g / piece of cookie was prepared. Table 5 Cookie composition containing both peptide and isoflavone (100 g) --- ------------------------------------------- Peptide-containing product 4.7 g Soybean hypocotyl ground product 1.0 g Soft flour 45. 6 g Margarine 27.5 g White sugar 17.6 g Liquid egg 3.0 g Salt 0.6 g ------------------------------------- ----- Total 100.0 g ---------------------------------- Soybean hypocotyl crushed product used Was an isoflavone-containing material "Soyaflavone-RS" (water content 5%, isoflavone content 1%. Fuji Oil Co., Ltd .; hereinafter the same). By ingesting 10 of these cookies, 3.5 g of peptide and 13 mg of isoflavone can be ingested. This cookie can be applied to a health food for the purpose of nutritional supplementation.

[0027]

Example 5 Tablet Containing Peptide and Isoflavone Raw materials were prepared according to the formulation shown in Table 6 to prepare tablets of 1 g / grain. Table 6 ------------------------------------------- Tablet formulation containing both peptide and isoflavone (100 g)- ------------------------------------------- Peptide-containing product 67.0 g Soybean hypocotyl extract 2.5 g Lactose 25. 5 g Calcium carbonate 2.0 g Emulsifier 3.0 g --------------------------------------------------------------------------------------- Total 100.0 g- ------------------------------------------------- By ingesting 10 tablets of the present tablet, 5.0 g of the peptide and It is possible to replenish 15 mg of isoflavones and 80 mg of calcium.
This tablet can be applied to health food for the purpose of nutritional supplementation.

[0028]

Example 6 Black Tea Beverage Containing Peptide and Isoflavone The raw materials were blended according to the formulation shown in Table 7 to prepare 100 g / cup of black tea beverage. Table 7 -------------------------------------------- A tea beverage formulation containing both peptide and isoflavone (100 g)- ----------------------------------------- Peptide-containing product 6.7 g Soybean hypocotyl extract 0.25 g Granulated black tea 0.60 g Whole milk powder 2.0 g Sugar 7.5 g Black tea flavor 0.03 g Water 82.92 g ------------------------------. ---------- Total 100.0 g ----------------------------- By ingesting 100 g, 5.0 g of peptide and 15 mg of isoflavone can be supplemented. This tea beverage can be applied to a health food for the purpose of nutritional supplementation.

From the results of Examples 1 and 2, the food or beverage containing both isoflavone and peptide has the effect of strengthening the bone itself by suppressing the decrease of the bone mass caused by osteoporosis, and further It was suggested that by strengthening the muscles that wrap around the body, it is possible to reduce the burden of excessive load on the bone and to expect a double effect of preventing fractures such as falls.

[0030]

INDUSTRIAL APPLICABILITY According to the present invention, foods and beverages containing both isoflavone and peptides were obtained. As a result, for example, soybean hypocotyl extract 0.2 with an isoflavone content of 4% by weight is obtained.
5 parts and soy peptide with a peptide content of 75% by weight 6.
7 parts were mixed with 200 parts of the beverage, and isoflavone 15 m
The beverage containing g and 5 g of peptide can provide a product that can be useful for strengthening bones and strengthening muscles that widely surround the bones, and can also contribute to an aging society.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI A23L 2/66 A23L 2/00 F (58) Fields investigated (Int.Cl. ⁷ , DB name) A23L 1/29-1 / 305 A23L 1/20 A23J 3/16-3/34

Claims (5)

(57) [Claims] 1. An isoflavone is added, and the isoflavone content is 0.4% or more based on the dry weight of the peptide, and the isoflavone- containing peptide is included.
Foods and beverages with a content of 2% by weight or more in terms of dry weight . 2. The food and beverage according to claim 1, wherein the isoflavone is obtained from soybean hypocotyl, its dry-heated product, or its water-soluble solvent extract or organic solvent extract. 3. The food and beverage according to claim 1 or 2, wherein the peptide is soybean peptide obtained by hydrolyzing isolated soybean protein with a protease. 4. The isoflavone used has a dry weight of isoflavone content of 1% by weight or more, and the peptide-containing substance used has a dry weight of protein content of 50% by weight or more. Listed food. 5. The method according to any one of claims 1 to 4, which contains a Ca (calcium) -containing material, minerals, vitamins, and functional materials other than isoflavone that have a Ca absorption promoting effect or a Ca elution suppressing effect. Food and drink.