JP3285680B2 - Plant culture vessel - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a container for culturing plants.
A seedling is formed by aseptically cultivating a plant seed or a plant tissue, and the seedling is transplanted and further aseptically cultured to grow to a fixed seedling. For such plant cultivation, a glass flask sealed with a cotton plug, aluminum foil, or the like is generally used. In recent years, relatively large plant culture vessels have also been used. The present invention relates to an improvement in such a plant culture vessel.

[0002]

2. Description of the Related Art Conventionally, a plant culturing vessel has a main body and a lid closely attached to the main body. The main body houses a growing basket of a plant, a support, and the like. Has also been proposed in which a top fertilizer is made available and a plant is aseptically cultured in a liquid medium loaded in the main body (JP-A-63-169983, JP-A-2-57131,
JP-A-2-154680). However, in these conventional plant culture vessels, for example, when transplanting seedlings on a clean bench and moving the plant culture vessel after transplantation to a clean room, or when changing the position of the plant culture vessel in a clean room, for example, And the liquid culture medium oscillates and undulates, and the liquid culture medium wets the stems and even the leaves of the transplanted seedlings. There is a disadvantage that they die.

[0003]

The problem to be solved by the present invention is that, in a conventional plant culture vessel, the liquid medium wets the stems and even the leaves of the seedlings when carrying them, so that the seedlings are poorly grown. Or die.

[0004]

SUMMARY OF THE INVENTION The present invention provides a container for culturing a plant in a liquid medium, comprising: a main body; a light-transmissive lid detachably attached to the main body; A liquid-permeable holder removably housed in the main body, a positioning stopper for the holder protruding from an inner peripheral bottom surface of the main body, a support for the seedlings housed in the holder, A ring-shaped edge protruding from the inner peripheral side surface of the main body below the surface, a liquid medium loaded into the main body below the edge, and an opening formed in the lid,
A breathable sterilizing filter that is in close contact with the opening, a topdressing pipe attached to the lid, and a liquid-permeable sterilizing filter interposed in the topdressing pipe are provided. And a stopper for preventing the holder from moving and displacing the liquid medium at the edge thereof.

[0005] A plant culture vessel according to the present invention includes a main body and a lid detachably attached to the main body. The lid is attached to the main body by attaching a lid to the main body, sealing both with adhesive tape, and forming a flange portion on both sides, and screwing or clamping the flange portion via packing. Although it is possible to make the cover detachably adhered, it is preferable that both the joints are formed in a male and female relationship, and the cover is detachably fitted to the body by pressing and fitting the cover to the body.

At least the lid, preferably also the body, is made of a light-transmitting rigid material. Both may be made of transparent or translucent glass, but those made of transparent or translucent plastics are preferable in terms of durability, light weight and economy, and among them, heat resistance that can be sterilized by autoclaving is preferred. The one made of polycarbonate is preferable in that it has.

[0007] A liquid-permeable holder is detachably housed in the main body. The holder is convenient for collectively storing the seedlings transplanted to the support into the main body, and removing the grown planted seedlings together with the support from the main body. The holder is made of a rigid material having liquid permeability. Such a holder may be a metal tray in which a plurality of holes are punched, but a metal mesh structure is preferable, and an aluminum or stainless steel mesh structure is particularly preferable.

[0008] A support is accommodated in the holder. The support is for supporting the transplanted seedlings. Examples of such a support include rock wool, ceramic wool, vermiculite, perlite, absorbent cotton, wood fiber, polyester fiber, and phenol resin foam. Rock wool is preferable, and plate-like rock wool is particularly preferable.

A ring-shaped edge protrudes below the surface of the support on the inner peripheral side surface of the main body. A holder is accommodated in the main body, a support is accommodated in the holder, and a ring-shaped edge protrudes from the inner peripheral side surface of the main body below the surface of the support. The liquid medium is loaded into the main body below the ring-shaped edge, and topped up, and absorbed through the root of the seedling transplanted to the support via the liquid-permeable holder and the support. Even when carrying the plant culture container in such a state, the ring-shaped edge suppresses the swinging and waving of the liquid medium, so that the liquid medium does not wet the stems and even the leaves of the seedlings transplanted to the support, Therefore, poor growth and withering of the seedlings can be prevented.

The ring-shaped edge and the holder are arranged so that the tip of the edge is substantially in contact with the outer peripheral side surface of the holder, and a stopper for positioning the holder is provided on the circumferential bottom surface of the main body. To protrude. This is because, when the plant culture container is carried, the holder is prevented from shifting and the rocking and waving of the liquid medium can be suppressed more reliably.

An opening is formed in the lid, and a gas permeable sterilizing filter is closely attached to the opening. For example, a disinfecting filter is attached to an opening of a wide mouth, and a peripheral portion of the disinfecting filter is sealed to a lid around the opening with an adhesive tape. A fertilizer pipe is attached to the lid, and a liquid-permeable sterilizing filter is interposed in the fertilizer pipe. This is because the necessary liquid medium can be top-fertilized as needed during the culture. Commercially available products (for example, Millipak (trade name) manufactured by Millipore Co., Ltd.) can be used as such a disinfecting filter. Also, a pipe for top fertilizer is divided, and a disinfecting filter is inserted between both flanges of the divided portion through packing. Can be used which is clamped by a clamp or the like.

As the liquid medium to be loaded into the main body and fertilized, a known medium can be used, but a sugar-free liquid medium is preferable. The sugar-free liquid medium is a liquid medium that does not contain saccharides such as sucrose, glucose, and fructose, and has the advantage of being less susceptible to bacterial contamination than a normal liquid medium containing saccharides. Such a sugar-free liquid medium can be prepared in the same manner as a normal liquid medium except that no saccharide is added (Japanese Patent Application Laid-Open No. 54-5 / 1979).
2787, JP-A-1-285117).

Among the sugar-free liquid media, pH 3.0 to 3.0
Those adjusted to 4.5 are preferred. In general, the pH of a liquid medium, including a sugar-free liquid medium, is adjusted to about 5.2 to 5.5. On the other hand, transplanting on a clean bench and culturing in a clean room do not mean that the clean bench or clean room is completely sterile. It is inevitable that various bacteria enter or enter the inside of the plant culture container, and even with a sugar-free liquid medium, the growth of such various bacteria cannot be reliably suppressed. A sugar-free liquid medium whose pH has been adjusted to 3.0 to 4.5 can reliably suppress the growth of mixed or invading germs. When the pH exceeds 4.5, the above-mentioned pH adjusting effect cannot be sufficiently obtained, and when the pH is less than 3.0, the growth of the seedlings is adversely affected.

Among the sugar-free liquid media, those containing an antibacterial agent are preferred. Such antibacterial agents include sodium hypochlorite, polylysine, penicillin, gentamicin and the like. The concentration in a sugar-free liquid medium is 0.1% in the case of sodium hypochlorite and polylysine.
-10 ppm, 10-100 pp for penicillin
m, in the case of gentamicin, the concentration is 1 to 100 ppm. This is the same reason as in the case of adjusting the pH described above.

In the present invention, the liquid medium to be loaded on the main body and fertilized is a sugar-free liquid medium,
Most preferably, H is adjusted to 3.0 to 4.5, and 0.1 to 10 ppm of sodium hypochlorite is contained as an antibacterial agent. Although contamination or invasion of various bacteria is particularly dangerous at the time of transplantation, sodium hypochlorite is effective in killing various bacteria that have entered or invaded at the time of transplantation,
In addition, pH adjustment can be effective to suppress the growth of mixed or invading bacteria after transplantation.

[0016]

A stopper for positioning the holder is provided on the inner bottom surface of the main body to prevent the holder from shifting when the plant culture container is carried, and a support housed in the main body together with the holder. A ring-shaped edge protrudes from the inner peripheral side surface of the main body below the surface of the body,
Since the margin suppresses rocking and waving of the liquid medium when carrying the plant culture container, the liquid medium does not wet the stems and leaves of the seedlings transplanted to the support, and therefore the poor growth of the seedlings And death can be prevented.

[0017]

FIG. 1 is a longitudinal sectional view showing an embodiment of the present invention. A transparent polycarbonate lid 21 is attached to a transparent polycarbonate body 11. Body 11
A ring-shaped concave portion 12 is formed on the upper end surface of the base member, and a ring-shaped flange 13 is provided on the outer peripheral surface of the end portion. A ring-shaped convex portion 22 is projected from the lower end surface of the lid 21, and a ring-shaped flange 2 is formed on the outer peripheral surface of the end portion.
3 are protruded. The convex part 22 is a ring-shaped packing 3.
The flange 13 and the flange 23 are fastened by clamps 41 and 42.

The main body 11 has a holder 5 made of a net-like structure.
1 are stored, and the holder 51 is positioned between the stopper 14 and the stopper 15 projecting from the inner peripheral bottom surface of the main body 11. The holder 51 houses a support 61 made of a plate-like rock wool.

A ring-shaped edge 16 protrudes from the inner peripheral side surface of the main body 11 below the surface of the support 61, and the tip of the edge 16 is close to the outer peripheral side surface of the holder 51. The main body 11 is loaded with the sugar-free liquid medium A below the edge 16.

The lid 21 is provided with a wide-opening 24, and an air-permeable disinfecting filter 71 is attached to the opening 24.
Is sealed with the adhesive tape 72 around the lid 21 around the cover. The top fertilizer pipe 81 is attached to the lid 21 so as to face between the inner peripheral side surface of the main body 11 and the holder 51, and the top fertilizer pipe 81 is divided into a pipe member 82 and a pipe member 83. The piping member 82 and the piping member 83 are screwed at both flanges, and a liquid-permeable sterilizing filter 84 is held between the two flanges via a packing.

[0021]

As is apparent from the above description, the present invention described above can suppress the fluctuation and undulation of the liquid medium when carrying a relatively large plant culture container, and therefore can prevent such fluctuation and undulation. This has the effect of preventing poor growth and withering of the seedlings caused by this.

[Brief description of the drawings]

FIG. 1 is a longitudinal sectional view showing one embodiment of the present invention.

[Explanation of symbols]

11 ... body, 14, 15 ... stopper, 16 ...
-Edge, 21 ... lid, 31 ... packing, 41,
42 ... clamp, 51 ... holder, 61 ... support, 71, 84 ... sterilization filter, 81 ... topdressing pipe

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-2-57131 (JP, A) JP-A-2-291254 (JP, A) JP-A-63-169983 (JP, A) JP-A-63-169983 313577 (JP, A) JP-A 2-142138 (JP, U) JP-A 1-155399 (JP, U) Table 2-500003 (JP, A) (58) Fields surveyed (Int. Cl. ⁷ , DB name) A01H 4/00 C12M 3/00

Claims (7)

(57) [Claims] 1. A container for culturing a plant in a liquid medium, comprising a main body, a light-transmissive lid detachably attached to the main body, and a liquid passage removably housed in the main body. A holder, a stopper for positioning the holder protruding from the inner bottom surface of the main body, a support for the seedlings stored in the holder, and an inner periphery of the main body below the surface of the support. A ring-shaped edge protruding from the side surface, a liquid culture medium loaded in the main body below the edge, an opening formed in the lid, and an air-permeable filter tightly attached to the opening. Bacterial filter, comprising a topdressing pipe attached to the lid, and a liquid-permeable sterilization filter interposed in the topdressing pipe,
A plant culturing container characterized in that the stopper prevents the holder from slipping and moving at the time of carrying, and suppresses swinging and waving of the liquid medium at the edge. 2. The plant culture container according to claim 1, wherein the lid is made of transparent or translucent plastics. 3. The plant culture vessel according to claim 1, wherein the holder is a metal mesh structure, and the support is rock wool. 4. The liquid medium according to claim 1, wherein the liquid medium is a sugar-free liquid medium.
4. The container for plant culture according to any one of items 3 to 3. 5. The container for plant culture according to claim 4, wherein the sugar-free liquid medium is adjusted to pH 3.0 to 4.5. 6. The container for plant culture according to claim 4, wherein the sugar-free liquid medium contains an antibacterial agent. 7. The container for plant culture according to claim 6, comprising 0.1 to 10 ppm of sodium hypochlorite as an antibacterial agent.