JPH0683662B2 - Plant culture vessel - Google Patents

Description

TECHNICAL FIELD The present invention relates to a plant incubator used for producing seedlings using a tissue culture method.

[Prior Art] The tissue culture method enables rapid mass production of plants, and
A large amount of seedlings necessary for commercial cultivation can be supplied from the mother stock of the book.

Most of the plants produced by the plant culture method are flowers and vegetables, but recently, this technology has also been used for vegetative propagation of fruit trees, timber, plantation crops, etc., and the market tends to expand further. It is in.

The growth process of tissue culture is based on establishment of a sterile culture system (stage I), growth of plants (stage II), and rooting and acclimation for soil transplantation (stage III). An example of this proliferation process is the procedure shown in the flowchart of FIG.

That is, first, a medium is prepared. The medium is prepared by mixing agar with nutrient components suitable for the target plant. Next, this medium is dispensed into a plurality of medium containers and stoppered with a cotton stopper, a rubber stopper with cotton, an aluminum foil or the like. Then, put the whole container in an autoclave and sterilize under high pressure. In this way, a sterile culture system is established (stage I).

This sterilized incubator is transferred to a clean bench, and the container is opened to transplant plant seeds or sterilized and sterilized plant tissue into the medium. Then, the stopper is plugged again and the incubator is placed in the clean room to start the culture. This culture is performed until the seeds become seedlings or until the seedlings are redifferentiated from the tissue (stage II).

Then, the seedlings are taken out from the incubator and acclimated to grow until planted seedlings (stage III).

This planting seedling is planted and grown as a cultivated plant. Thus, in tissue culture, all of stages I to III are carried out under aseptic conditions.

As the incubator used at this time, a glass container which is a pressure-resistant and heat-resistant container is used because it is necessary to sterilize by high pressure in an autoclave, and a stopper having a certain degree of air permeability is used as the stopper of the incubator. The reason why this plug has air permeability is to replenish the incubator with carbon dioxide gas required for photosynthesis during the growth process of the seedlings.

In this way, the incubator requires a vent for replenishing carbon dioxide gas, but in order to avoid microbial contamination during culturing, the glass container is as narrow as possible, such as an Erlenmeyer flask and a test tube. Is used.

In addition to the above-mentioned container, the incubator is made of a gas-permeable and light-permeable fluororesin film (made by Daikin Industries), and a stainless steel frame that forms the outer shell of the container is placed inside the bag and the opening of the bag is heat-welded. Then, a sealed container has been developed.

[Problems to be Solved by the Invention] However, since the glass container has a small culture area in the case of a test tube and has a narrow mouth in the case of an Erlenmeyer flask, the glass container has a small number of cells when transitioning to the stage III habituation of tissue culture. It is not easy to take out the plant from the Erlenmeyer flask, which not only deteriorates workability but also damages the young plant.

In addition, the above-mentioned glass container has a container opening stoppered with a cotton stopper, a rubber stopper with cotton, an aluminum foil or the like. However, since the effect of preventing microbial contamination at the opening is incomplete, culture stages I to All of III must be performed in a clean room, some equipment is required, and it is difficult to transfer to the outside during the culture.

Further, the container using the fluororesin film can have a wide design of the opening of the container, and can completely seal the opening, so that there are some merits.
There is a problem that the gas permeability is insufficient and the amount of carbon dioxide gas required for the growth of seedlings is insufficient to be introduced into the incubator.

The present invention has been made in view of the above-mentioned circumstances, and the purpose thereof is to easily take out seedlings during acclimation, effectively prevent microbial contamination, and sufficiently introduce carbon dioxide into the incubator. The present invention is to provide an inexpensive and disposable plant culture container that can be secured in the above.

[Means for Solving the Problems] In order to achieve the above-mentioned object, the plant culture container according to the present invention has a bottomed cylindrical shape made of a transparent plastic thin plate made of vinyl chloride resin or acrylic resin having a thickness of 0.2 to 1 mm. A medium container, a lid made of the plastic thin plate and closely attached to the opening so as to cover the opening of the container, and an outer wall of the medium container and at least one intake hole in each of the lids, It is characterized by comprising a micro filter fixed to the intake hole so as to cover the intake hole.

[Operation] Since the bottomed cylindrical medium container and lid are thin plastic, the upper part of the container or the lid can be easily cut off when a young plant is taken out, and a wide opening can be opened. , Easy to take out when acclimatizing young plants.

By constructing the container with a transparent plastic thin plate made of vinyl chloride resin or acrylic resin with a thickness of 0.2 to 1 mm, the container expands or contracts according to the change of the internal pressure inside the container due to the temperature change, as if the container itself. Shows the state of breathing and promotes the introduction of carbon dioxide through the microfilter. In this way, since the outside air enters the container aseptically, the same environment as outside the container can be aseptically created inside the container.

The microfilter not only allows introduction of carbon dioxide gas into the container but also prevents microorganisms from entering the container and maintains the inside of the container in a sterile condition.

[Example] FIG. 1 shows a plant culture vessel 1 according to the present invention.

The culture container 1 is composed of a bottomed cylindrical medium container 2 and a lid 3 that covers the opening 2a of the medium container 2. The medium container 2 has a bottom portion 2b that is curved so as to be convex inward, and a base end side is continuously connected to the outer periphery of the bottom portion 2b so as to stand up in a slightly divergent shape, and an outer wall with an opening 2a at the upper end. 2c and a flange portion 2d having an L-shaped cross section, the base end side of which is connected to the outer periphery of the opening 2a and which is formed to project outward.

On the other hand, the lid body 3 has a gentle conical covering portion 3a that covers the opening 2a of the culture medium container 2 and a base end side of the outer periphery of the covering portion 3a, which is continuously formed so as to project downward. It has an open U-shaped flange portion 3b.

Further, the medium container 2 and the lid 3 are provided with suction / air absorption holes 4 at appropriate places. The intake holes 4 are for introducing carbon dioxide gas into the culture container 1. In this embodiment, one intake hole 4 is provided on each of the upper portion of the outer wall 2c of the culture medium container 2 and the top of the covering portion 3a of the lid 3. ing. In the present embodiment, the intake hole 4 is formed as a large hole having a diameter of 10 mm or more, and the water in the incubator 1 evaporates to dry the medium.

The culture medium container 2 and the lid 3 are molded from a transparent plastic material, such as vinyl chloride resin or acrylic resin, by a conventional resin molding machine so as to have a thickness of about 0.2 to 1 mm. Since such a synthetic resin material has the characteristics of γ-ray resistance and sterilization gas (ethylene oxide) resistance, sterilization treatment is possible with these.

The intake holes 4 of the culture medium container 2 and the lid 3 are microfilters 5.
Is covered with. This microfilter 5 is a culture container 1
It allows the introduction of carbon dioxide into the inside and prevents the introduction of microorganisms, and is composed of a synthetic resin thin plate having a large number of 0.1-0.2 micron holes formed therethrough. If the size of the through holes exceeds 0.2 microns, the function of preventing microbial contamination is significantly reduced, which is not preferable.
As the material of the microfilter 5, a plastic material having the same characteristics as the medium container 2 and the lid 3 described above is used. The microfilter 5 is attached to the air intake hole 4 after the culture medium container 2 and the lid 3 are molded. The tissue culture of a plant using the thus formed medium container 2 and lid 3 is performed as follows.

First, the culture medium container 2 and the lid 3 are placed in γ-ray irradiation and an ethylene oxide gas atmosphere and sterilized.
Thereafter, the medium container 2 and the lid 3 are transferred to a clean bench, and the medium 6 sterilized in advance in the bench is dispensed into the medium container 2 and the plant body 7 maintained in a sterile state in the medium 6 is dispensed. Transplant. The medium container 2 into which the plant 7 is transplanted in this way is sealed with the lid 3. This sealing is performed by fitting the flange portion 3b of the lid 3 into the flange portion 2d of the culture medium container 2 and bringing the flanges 2d and 3b into close contact with each other. The flanges 2d and 3b may be adhered to each other via an appropriate adhesive agent, or may be heat fusion or solvent fusion of the flanges 2d and 3d. In this way, the plant 7
The culture vessel 1 into which the bacterium was transplanted is placed in an environment in which temperature and light are obtained within a growth-appropriate range for the plant, and the culture is started. That is, the culture container 1 may be placed not only in a clean room but also in a normal greenhouse to start the culture.

The inside of the culture container 1 is maintained aseptic, and external carbon dioxide gas is introduced into the inside through the microfilter 5. In addition, since the culture container 1 is made of a synthetic resin thin plate, the culture container 1 expands or contracts due to a change in internal pressure due to a temperature change in the culture process, and the container 1 is in a state of breathing. And the growth of the plant body 7 is promoted.

When the plant body 7 has grown into a seedling, the plantlet is taken out of the culture container 1 and the next acclimatization step is performed. After this acclimatization step, it is performed in the same manner as the conventional one.

To take out the seedlings from the culture container 1, the cover 3a of the lid 3 is cut out and opened to a size substantially equal to the opening 2a of the medium container 2. The excision of the covering portion 3a can be easily performed because the culture container 1 is made of a synthetic resin thin plate.

The opening of the culture container 1 opened in this way is wide,
The seedlings can be easily taken out without destroying the shape of the plant or damaging it.

In the present embodiment, the cover portion 3a of the lid body 3 is formed in a conical shape in order to prevent water droplets attached to the inside from falling due to its own weight and lowering the light transmittance due to water droplet attachment. The prevention of this water drop attachment can also be achieved by a drip-proof treatment by laser processing.

Further, the bottom 2b of the medium container 2 is curved so as to be convex inward in order to stabilize the containers 2 when they are stacked, and the sterilization process can be performed in the stacked state. is there.

The present invention is not limited to the embodiments described above, and various modifications can be made without departing from the spirit of the invention.

For example, the flange 2d provided on the medium container 2 and the lid 3
3b may be flat or may be a system in which the medium container and the lid are fitted to each other without providing these flanges.

[Effect of the Invention] In the plant culture container of the present invention, as described above, the opening of the medium container is sealed with a lid, and carbon dioxide gas is introduced into the container through a microfilter that prevents the introduction of microorganisms. Therefore, the plant can be cultured in a sterile state free from microbial contamination and in a sufficient carbon dioxide atmosphere.

Further, since the medium container of the present invention is a cylindrical container with a bottom, the opening is widened, and it is easy to take out the young plant at the time of acclimation and the workability is improved, and the shape of the plant is destroyed or damaged. Can be done without

Further, in the present invention, since the container is made of a synthetic resin thin plate, the amount of the resin is small, so that the container can be manufactured inexpensively to the extent that it can be disposable. It is possible to secure the sufficient amount of carbon dioxide gas in the container by facilitating the introduction of carbon dioxide gas through the microfilter by exhibiting the state that the container itself is breathing.

By sufficiently introducing carbon dioxide into the vessel in this manner, the following can be expected.

That is, under the condition that the effective photosynthetic radiation amount is not a limiting factor, the photosynthetic rate increases and the growth of seedlings is promoted. Increasing autotrophic growth of seedlings in this way enhances adaptability during acclimation and significantly reduces the failure rate.

When opening the plant culture container, the lid and the container are formed of a synthetic resin thin plate so that the plant culture container can be easily cut out, and the work of taking out the young plant at the time of acclimatization is further improved.

Further, when the sugar concentration is lowered at the time of rooting, the features of the present invention can be fully exerted. That is, when the sugar concentration is low,
The sugar concentration becomes 0 at the stage of re-differentiation and removal from the container, and the reproduction of mold and the like can be suppressed without sufficiently dropping the medium.

[Brief description of drawings]

FIG. 1 is a sectional view showing an embodiment of a plant culture container of the present invention,
FIG. 2 is a flow chart illustrating a conventional culture process for plant tissue culture. 1 ... Plant culture container, 2 ... Medium container, 3 ... Lid, 4
...... Intake hole, 5 ... Micro filter, 6 ... Medium, 7
...... Plants.

Claims (1)

[Claims] 1. A bottomed cylindrical culture medium container made of a transparent plastic thin plate made of vinyl chloride resin or acrylic resin having a thickness of 0.2 to 1 mm, and the opening made of the plastic thin plate so as to cover the opening of the container. And a microfilter that has at least one intake hole on each of the outer wall of the culture medium container and the cover and is fixed to the intake hole so as to cover the intake hole. A plant culture container characterized by: