JP3100300U - Solid state fermentation tank - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a tank for solid-state fermentation capable of reducing man-hours and cost of operation and improving the production of bacterial cells.
SOLUTION: The tank 1 includes a tank 1 and a lid 2, the tank 1 has an integrated outer tank 11 and an inner tank 12, and a back peripheral surface of the outer tank 11 and a front peripheral surface of the inner tank 12. A first space 13 is formed between the inner tank 12 and the inner space of the inner tank 12 is a second space 14, and a plurality of radially equidistant notches 113 are provided at a top edge of the outer tank 11. The lid 2 covers the top surface of the tank 1 from above, and a number of notches 21 corresponding to the notches 113 of the outer tank 11 are provided on the periphery of the lid 2. A plurality of viewing windows 22 for monitoring the second space 14 of the inner tank 12 are provided on the top surface.
[Selection diagram] Fig. 1

Description

The present invention relates to a tank for solid-state fermentation, and in particular, it is possible to reduce the number of man-hours required for the propagation of bacterial species in solid-state fermentation and the production of biologically active substances by an automated method, and to reduce the amount of cells produced. The present invention relates to a solid state fermentation tank that can be improved.

生物 Biologics are developed to combat pests to overcome the harm of traditional chemical pesticides to humans and the natural environment. The primary function of the biologic is to infect microorganisms with the pest and the pest dies from the infection, and the selected microorganism normally infects the target pest but does not harm the non-target organism.

However, there are many factors to consider in the research and development of biologics with potential, but successful commercialization depends on the following two factors. These include the development of technologies that can be mass-produced at low cost and the competitiveness of product prices. And the competitiveness of products is determined by the development of technologies that can be mass-produced at low cost.

Nomuraea rileyi was able to infect many pests in its natural environment and died after the pests were infected. According to the literature, Nomuraea rileyi can parasitize 30 kinds of pests, and the mortality against the night moths is particularly strong, and often causes the epidemic of the night moths. Of particular note, the Nomuraea rileyi has a 42-day surface half-life on the surface, which is considered to be a very potent biologic for the eradication of night moth pests. In the United States, the Nomuraea rileyi is applied to eliminate nymphs of the night moth family pests that harm soybeans, and the Nomuraea rileyi is used in a vast area using the Nomuraea rileyi. Caused a pest epidemic with significant effects.

However, the method of culturing Nomuraea rileyi generally involves first filling rice, starch, and other nutrients, such as a mixture of ground fish and yeast powder, into a space bag. After inoculating Nomuraea rileyi into a space bag and culturing it, supplying sufficient light to illuminate the sun and controlling the temperature and humidity of the space bag, the Nomuraea rileyi (Nomuraea rileyi) Can be cultured.

This has the following disadvantages.
(A) According to the above method, it is necessary to inoculate Nomuraea rileyi on each space bag, and it is necessary to duplicate the work, so that the number of man-hours is increased.
(B) Since mass production cannot be performed by an automated production method, if the required amount increases, the production amount cannot be kept in time.

The present invention has been made in view of the above problems, and its main purpose is to cover a lid on a tank, and to stir the culture material in an inner tank with a stirrer, and to determine a temperature, a humidity, a stirring speed and An object of the present invention is to provide a solid-state fermentation tank capable of reducing the number of man-hours and cost of operation and improving the production amount of bacterial cells by automatically adjusting the brightness and the amount of ventilation.

Claim 1 of the present application made in order to achieve the above object is a solid state fermentation tank including a tank and a lid, wherein the tank has an integrated outer tank and inner tank, and the outer tank A first space is formed between the back peripheral surface of the inner tank and the outer peripheral surface of the inner tank, the inside of the inner tank is a second space, and the top edge of the outer tank is radially equidistant. A plurality of notches are provided, fixed parts are pivotally provided below each notch, the outer tank has two inlets, and the end of each of the inlets inside the outer tank is provided. Is connected to the communication pipe separately in the first space and presents a cross shape, and each of the communication pipes is provided with a number of holes separately equidistant and provided outside the outer tank. A distribution pipe is connected between the other ends of the entrances, and the distribution pipe is provided with a steam pipe, a frozen water pipe, and a room temperature water pipe, and the lid is provided for the tank from above. A number of notches corresponding to the notches of the outer tank are provided on the peripheral edge of the lid, and a plurality of notches for monitoring the second space of the inner tank are provided on the top surface of the lid. A window is provided, and the lid is provided with a stirrer, a sampler, and an inoculator, and the gist is a tank for solid-state fermentation.

Please refer to FIG. 1 to FIG. The embodiment of the present invention is a solid state fermentation tank including a tank 1 and a lid 2.
The tank 1 has an integrated outer tank 11 and an inner tank 12, and a first space 13 is formed between a back peripheral surface of the outer tank 11 and a front peripheral surface of the inner tank 12, The inside of the inner tank 12 is a second space 14.

The bottom of the outer tank 11 communicates with the first space 13 and has a leak hole 15 for leaking water in the first space 13. The opening and closing of the leak hole 15 is controlled by a valve 151, A plurality of overflow pipes 16 are provided in the vicinity of the top edge of the outer tank 11 for communicating with the first space 13 and discharging excess water in the first space 13. The opening and closing of the pipe 16 is jointly controlled by a valve (not shown).

At the top end of the outer tank 11, there is an annular groove 111, and a packing 112 for maintaining airtightness when the lid 2 is covered is fitted in the annular groove 111. A plurality of notches 113 which are radial and equidistant are provided on the edge, and fixed components 3 are pivotally provided below each notch 113. In this embodiment, the fixed components 3 are sleeves 32. A bolt 33 for pivoting is provided below the notch 113, and a ring 33 is provided on the opposite side of the sleeve 32 in the tightening direction.

The outer tank 11 has two inlets 4, and the end of each of the inlets 4 inside the outer tank 11 is connected to the communication pipe 41 separately in the first space 13 to form a cross shape. The communication pipe 41 is provided with a large number of holes 411 which are separately equidistant, and a distribution pipe 42 is connected between the other ends of the inlets 4 outside the outer tank 11. The distribution pipe 42 is provided with a steam pipe 43, a frozen water pipe 44, and a room temperature water pipe 45.

A thermometer 17 for sensing the temperature of the inner tank 12 is provided at the bottom of the second space 13 of the inner tank 12, and one end of the thermometer 17 extends to the outer edge of the outer tank 11. .
The lid 2 covers the top surface of the tank 1 from above, and a number of notches 21 corresponding to the notches 113 of the outer tank 11 are provided on the periphery of the lid 2, and the notches 21 The lid 2 is fixed to the tank 1 when the sleeve 32 is tightened after turning the bolt 31 of the fixed component 3 upward, corresponding to the fixed component 3. A plurality of viewing windows 22 for monitoring the second space 14 of the inner tank 12 are provided on the top surface of the lid 2.

As shown in FIGS. 3 and 4, a stirrer 5 is provided on the top surface of the lid 2. In this embodiment, the stirrer 5 has a first motor 51 and a second motor 56. The first motor 51 is fixed to the top surface of the lid 2, the second motor 56 is provided on a side surface of the first motor 51, and the first motor 51 is connected to the second space 14 of the inner tank 12. The indirect shaft 53 is provided at the bottom end of the rotational shaft 52, and horizontal shafts 54 are separately provided at both ends of the indirect shaft 53. 54, a plurality of blades 55 are separately provided, and the indirect shaft 53 is also connected to the second motor 56. By driving the first and second motors 51 and 56, the blade 55 is moved in the axial direction and the radial direction. And the contents of the second space 14 are stirred.

As shown in FIG. 5, a sampler 6 is provided on the lid 2. In this embodiment, the sampler 6 has hollow upper and lower sleeves 61 and 62, and one end of the lower sleeve 62. Is fixed to the lid 2, penetrates the bottom of the lid 2, the other end of the lower sleeve 62 is connected to the upper sleeve 61 by a connecting part 63, and among the upper and lower sleeves 61, 62, A sampling rod 64 is provided so as to extend and retract so as to be able to extend into the two spaces 14, and has a sampling port 641 for sampling at the bottom end of the sampling rod 64. A valve 65 and a steam joint 68 are provided at one end close to the component 63, and are close to the connection component 63 of the lower sleeve 62. At one end, a valve 66 and a steam joint 67 are provided. The valve 66 is provided to open and close the internal space of the front lower sleeve 62, and the steam joints 67 and 68 supply steam. It is provided to be introduced into the upper and lower sleeves 61 and 62 to kill bacteria.

As shown in FIG. 6, an inoculator 7 is provided on the lid 2. In this embodiment, the inoculator 7 has an inoculation tube 71, and one end of the inoculation tube 71 is A plurality of pipelines 72 (as shown in FIG. 7) penetrate the bottom of the lid 2 and distribute the bacterial species on average, are distributed evenly above the second space 14, on both sides of the inoculation tube 71. Are provided with steam tubes 73 for separately introducing steam into the inoculation tube 71 to kill bacteria. Each of the steam tubes 73 is separately controlled by a valve 731 and the lid of the inoculation tube 71 is provided. 2 is provided with a control valve 74 for controlling the opening and closing of the inoculation tube 71 and the pipeline 72 at the bottom of the lid 2. Covered by a protective lid 75 to protect the That.

When culturing the bacterial species using the embodiment of the present invention, it is necessary to perform a pre-operation, that is, a boiler, an air compressor, a controller, a chiller (including a heater), The controller (not shown) of a machine such as a dryer and a switch of a claim is turned on, and the valve 151 of the leak hole 15 of the tank 1 is opened to open the first space 13 of the outer tank 11 of the tank 1. After discharging the remaining water, the valve 151 of the leak hole 15 is closed, then the steam pipe 43 in the distribution pipe 42 is opened, and the steam is passed through the communication pipe 41 to the first space 13. Introduce and preheat.

Also, a fixing part 3 for fixing the lid 2 and the tank 1 is removed. When the fixing part 3 is removed, if the sleeve 32 is loosened directly, the bolt 31 can pivot downward so that the bolt 31 is detached from the notch 21 of the lid 2. 2 is opened, the prepared basic substance is placed in the second space 14 of the inner tank 12, and then the lid 2 is put back again, and the lid 2 and the tank 1 are locked by the fixing part 3, and After the pipe is connected, the valve 151 of the leak hole 15 is opened to discharge the condensed water generated during the preheating.

Immediately after the preparatory work, the sterilization work is started. First, the sterilization conditions such as the pressure in the first space 13 of the outer tank 11, the sterilization time, the sterilization temperature, and the time for entering the frozen water are set. Then, the automatic sterilizer (not shown) is turned on, and then the steam starts sterilizing the outer tank 11, the inner tank 12, the sampling device 6, the inoculator 7, and the like.
The basic substance is a mixture of yeast powder, molasses, crushed fish, water, and the like, which are suitable for grains. This base material provides high nutrient content to the inoculated fungal spores and allows spores to be produced in large quantities.

Thereafter, the first motor 51 of the stirring device 5 is started, and the first motor 51 drives the rotary shaft 52 to rotate the horizontal shaft 54 in the axial direction. Here, the second motor 56 also drives the indirect shaft 53. Then, the basic substance in the second space 14 of the inner tank 12 can be agitated on average, and the condition is monitored by the viewing window 22 in the lid 2. When the mixture is stirred to the average, the steaming process starts immediately. After setting the steaming time and the temperature, the steam pipe 43 in the distribution pipe 42 is automatically opened and the steam is injected into the first space 13. Then, the inner tank 12 is heated. When the steaming step is completed, the normal temperature water pipe 45 and the freezing water pipe 44 in the distribution pipe 42 automatically adjust the water input amount in order to maintain the culture environment of the basic substance in the second space 14 at a constant temperature. Then, the temperature of the second space 14 of the inner tank 12 is maintained.

Thereafter, in the inoculation step, first, the protective cover 75 of the inoculator 7 is opened, the flexible tube connected to the inoculation solution is quickly fitted into the inoculation tube 71, and the control valve 74 of the inoculation tube 71 is opened. The inoculum is injected from the inoculation tube 71, and the inoculum is evenly spread on the basic substance to be cultured by the pipeline 72. Then, the stirring device 5 is started to activate the inoculum and the basic substance to be cultured. Increase the contact area. When the inoculation process is completed, first, the control valve 74 is closed, the valve 731 of the steam pipe 73 is opened, and the inoculator 7 is sterilized so that the next inoculation or replenishment of the inoculation solution can be used. Is applied.

Thereafter, in the sampling step, after culturing for a certain period, a sample can be taken by the sampler 6, and when sampling, the sampling rod 64 extends into the basic substance in the second space 14 of the inner tank 12. So that the valves 65 and 66 of the upper and lower sleeves 61 and 62 are simultaneously opened, a sample is taken through the sampling port 641, and then the sampling rod 64 is pulled into the upper sleeve 61. By closing the valve 65 and opening the connecting part 63 so that the sample taken by the sampling rod 64 does not come into contact with the outside, the sampling rod 64 is moved together with the upper sleeve 61 into the lower part. Remove from the sleeve 62 and send it to the chemical analysis room to analyze the culture status of the bacterial species. .

According to the tank for solid-state fermentation of the present invention, the lid is firmly covered on the tank, and the culture material in the inner tank is stirred by the stirring device. By automatically adjusting the airflow and the amount of ventilation, it is possible to reduce the number of man-hours and cost of the operation, and to improve the production of bacterial cells.

FIG. 2 is an exploded perspective view showing a solid state fermentation tank according to an embodiment of the present invention. 1 is an external perspective view illustrating a solid state fermentation tank according to an embodiment of the present invention. FIG. 2 is a cross-sectional view illustrating a solid state fermentation tank according to an embodiment of the present invention. FIG. 2 is a top view showing the solid state fermentation tank according to the embodiment of the present invention. FIG. 2 is a side view showing a sampler of the solid state fermentation tank according to the embodiment of the present invention. FIG. 3 is a cross-sectional view illustrating the inoculator of the tank for solid-state fermentation according to an embodiment of the present invention. FIG. 2 is a top view showing a lid of the solid state fermentation tank according to the embodiment of the present invention.

Explanation of reference numerals

1 tank, 11 outer tank, 111 groove, 112 packing, 113 notch, 12 inner tank, 13 first space, 14 second space, 15 leak hole, 151 valve, 16 overflow pipe, 17 thermometer, 2 lid, 21 notch , 22 viewing window, 3 部品 fixed parts, 31 bolt, 32 sleeve, 33 ring, 4 entrance, 41 communication pipe, 411 hole, 42 pipe, 43 steam pipe, 44 freezing water pipe, 45 cold water pipe, 5 stirrer, 51 One motor, 52 rotary shaft, 53 indirect shaft, 54 horizontal shaft, 55 blade, 56 second motor, 6 sampling device, 61 upper sleeve, 62 lower sleeve, 63 connection parts, 64 sampling rod, 641 sampling port, 65, 66 Valve, 67, 68 steam joint, 7 inoculation , 71 inoculation tube 72 pipeline, 73 the steam pipe, 731 valve, 74 control valve, 75 a protective cover

Claims (7)

In a tank for solid state fermentation comprising a tank and a lid,
The tank has an integrated outer tank and an inner tank, and a first space is formed between a back peripheral surface of the outer tank and a front peripheral surface of the inner tank. It is two spaces, a plurality of notches which are radial and equidistant are provided at the top edge of the outer tank, and fixed parts are pivotally provided under each notch, and the outer tank is It has two entrances, and the end of each entrance inside the outer tank is connected to a communication pipe separately in the first space to form a cross shape, and each communication pipe is separately equidistant. A number of holes are provided, and a distribution pipe is connected between the other ends of the entrances outside the outer tank. The distribution pipe includes a steam pipe, a frozen water pipe, and a room temperature water pipe. And is provided
The lid covers the top surface of the tank from above, and a number of notches corresponding to the notches of the outer tank are provided on the periphery of the lid, and a second surface of the inner tank is provided on the top surface of the lid. A tank for solid-state fermentation, wherein a plurality of viewing windows for monitoring a space are provided, and the lid is provided with a stirring device, a sampling device, and an inoculation device. The fixing part is provided with a bolt for fastening with a sleeve under the notch, and a ring is provided on the opposite side of the fastening direction of the sleeve. 2. The solid state fermentation tank according to claim 1, wherein the lid is fixed to the tank when the tank is tightened. The bottom of the outer tank has a leak hole communicating with the first space, and the opening and closing of the leak hole is controlled by a valve, and the periphery near the top edge of the outer tank communicates with the first space. The solid state fermentation tank according to claim 1, wherein a plurality of overflow pipes are provided, and the opening and closing of each overflow pipe is jointly controlled by a valve. A thermometer for sensing a temperature of the inner tank is provided at a bottom of the second space of the inner tank, and one end of the thermometer extends to an outer edge of the outer tank. Item 4. The solid state fermentation tank according to Item 1. The stirrer has a first motor and a second motor, the first motor is fixed to the top surface of the lid, the second motor is provided on the side of the first motor, the first motor is A rotating shaft extending into the second space of the inner tank, an indirect shaft at a bottom end of the rotating shaft, and a horizontal shaft separately provided at both ends of the indirect shaft; A plurality of blades are separately provided on the horizontal axis, the indirect shaft is also connected to the second motor, and the blades are rotated in the axial direction and the radial direction by driving the first and second motors. The solid state fermentation tank according to claim 1, wherein the contents of the second space are agitated. The sampler has a hollow upper sleeve and a lower sleeve, one end of the lower sleeve is fixed to the lid and penetrates the bottom of the lid, and the other end of the lower sleeve is connected to the upper sleeve by a connecting part. A sampling rod is provided in the upper sleeve and the lower sleeve so as to extend and retract so as to be able to extend into the second space of the inner tank, and a sampling port is provided at a bottom end of the sampling rod. A valve and a steam joint are provided at one end of the upper sleeve adjacent to the connection part, and a valve and a steam joint are provided at one end of the lower sleeve adjacent to the connection part. The tank for solid-state fermentation according to claim 1, wherein: The inoculator has an inoculation tube, and one end of the inoculation tube is provided with a plurality of pipelines that penetrate the bottom of the lid and are distributed evenly on both sides of the inoculation tube. Each steam pipe is separately controlled by a valve, and a control valve is provided at an end of the inoculation tube close to the lid, and an upper end of the inoculation tube is covered with a protective lid. The solid state fermentation tank according to claim 1, wherein:

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