JP3093124B2 - Biochemical / immune automatic analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic biochemical / immune analyzer for analyzing a sample by dispensing a reagent and a sample into a reaction cell while rotating a rotary reactor, stirring and reacting the sample.

[0002]

2. Description of the Related Art Used in clinical, chemical and pharmaceutical fields,
In a biochemical analyzer that analyzes a sample such as serum or urine, a sample such as serum and a predetermined reagent are mixed in a transparent reaction cell to cause a color reaction, and white light is irradiated from a light source. The transmitted light is separated by a spectroscope, and the absorbance of the dye is determined, and then the concentration of the substance to be measured is determined based on the absorbance.

FIG. 5 is a diagram showing an outline of a system configuration of a conventional biochemical analyzer. Reference numeral 11 denotes a cup transport unit, 12 denotes a sample pipette, 13 denotes a sampling valve, 14 denotes a switching valve, 15 denotes a cool box, and 16 denotes a cool box. Reagent pump, 17
Denotes a reagent switching valve, 18 denotes a rotary reactor, 19 denotes a detection unit, 20 denotes an analyzer, and 21 denotes a data processing unit.

In FIG. 5, a rotary reactor 18 holds 30 to 40 reaction cells on its circumference, and performs cleaning of the reaction cells and measurement of a cell blank while rotating in, for example, 12 seconds per step. It is. First, a certain amount (1 μl to 40 μl) of each reaction cell of the rotary reactor 18 is passed through the sample pipette 12 from the cup transport unit 11.
A sample (sample) such as serum or urine (about 1 liter) is weighed and guided to the sampling valve 13, from which the first reagent and the sample are dispensed, and the second reagent is also dispensed. In this manner, when a sample such as serum and a predetermined reagent are mixed in a transparent reaction cell using the rotary reactor 18 to cause a color reaction, the detection unit 19 emits white light from a light source. The transmitted light is split by a spectroscope. The measurement data of the detection unit 19 is converted from an analog signal to a digital signal by the analysis unit 20, processed by the data processing unit 21, stored in a memory, displayed on a CRT, or output from a printer.

[0005] In recent years, in addition to the conventional biochemical measurement items, the following measurement items and reagents that require stirring intensity have begun to be used. These include immunoturbidimetry items, unadjusted reagents for biochemistry, and academically compliant reagents with high substrate concentrations. In addition, due to differences in liquid properties due to the concentration of the surfactant and the like, items that result in poor measurement when the stirring is increased and items that cannot be measured accurately without increasing the stirring have been classified.

[0006]

However, by controlling the number of rotations and the up and down movement of the stirring with a stirring rod or a stirring blade, it is possible to perform the optimum stirring according to the measurement item and the reaction reagent. No biochemical / immune automatic analyzer has a function capable of selecting and controlling a plurality of stirring intensities preset for each item. For this reason, there is a problem that it is not possible to cope with measurement items and reagents that require various stirring intensities as described above.In addition, when the stirring intensity is fixed as in the conventional apparatus, depending on the measurement items and measurement reagents, However, there is a problem that poor stirring and excessive stirring are caused, and measurement with high accuracy and precision cannot be performed.

An object of the present invention is to solve the above-mentioned problems, and an object of the present invention is to provide an automatic biochemical / immune analyzer capable of easily selecting and adjusting the stirring intensity according to an analysis item or the like. Is what you do.

[0008]

SUMMARY OF THE INVENTION For this purpose, the present invention provides a biochemical method for dispensing a reagent and a sample into a reaction cell while rotating a rotary reactor, stirring and reacting the sample to analyze the sample.
The automatic immunoanalyzer includes a compressed air line connected to the reaction cell at the stirring position, and an electromagnetic valve for adjusting the amount of air supplied from the compressed air line to the reaction cell. It was configured to adjust the stirring intensity by adjusting the supply amount, and before the compressed air line was connected to the reaction cell, air was temporarily stored in the air reservoir of the line from the solenoid valve to the reaction cell, and the air was stored. The stirring is performed by supplying air.

[0009]

According to the biochemical / immune automatic analyzer of the present invention, a compressed air line connected to a reaction cell at a stirring position is provided.
An electromagnetic valve for adjusting the amount of air supplied to the reaction cell from the compressed air line, and the stirring intensity is adjusted by adjusting the supply amount of air according to the opening and closing time of the electromagnetic valve. A plurality of stirring conditions can be set in advance for each analysis item, and a plurality of stirring conditions can be selected according to the analysis item.
In addition, before the compressed air line is connected to the reaction cell, air is temporarily stored in the air reservoir of the line from the solenoid valve to the reaction cell, and the stored air is used for stirring, so that the air in the air reservoir is discharged. The stirring intensity can be adjusted by the supply time, and the stirring intensity can be easily controlled.

[0010]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a view for explaining an embodiment of the automatic biochemical / immune analyzer according to the present invention, and FIG. 2 is a view for explaining the operation / timing of the automatic biochemical / immune analyzer according to the present invention. 1A to 1C are reaction cells, 2 is a rotor of a rotary reactor, 3 is a stator of a rotary reactor, 4 is a capillary, and 5 is a solenoid valve.

In FIG. 1, reaction cells 1A to 1C are held by a rotor 2 of a rotary reactor which relatively moves in a direction indicated by an arrow on a stator 3 of the rotary reactor as the rotary reactor rotates. A reagent and a sample are dispensed, and a second reagent is further dispensed. After the first reagent and the sample are dispensed, a second reagent is dispensed at a point, and compressed air is supplied at a point. Stirring. The electromagnetic valve 5 is connected to the compressed air line, and adjusts the amount of compressed air supplied at a point for stirring the reaction cells 1A to 1C. The stirring intensity is changed according to the opening and closing time of the solenoid valve 5. It is like that. The capillary 4 is connected to a line between the solenoid valve 5 and a connection point of the reaction cells 1A to 1C, and uses the line between the lines as an air reservoir.
The solenoid valve 5 is opened and closed at the position shown in FIG. 1 (a) to temporarily store compressed air, and this is supplied to the reaction cell at the position shown in FIG. 1 (b).

The operation of one cycle of the rotary reactor is, for example, from the position of the reaction cell 1A to the position of the reaction cell 1B, and takes, for example, 12 seconds. Take the position shown in (b).
The position shown in FIG. 1A is a position where the reaction cells 1A to 1C are not connected to the compressed air line of the solenoid valve 5, and the position shown in FIG. 1B is a reaction cell 1A to 1C.
C is a position at which the compressed air line of the solenoid valve 5 is connected to each other. If one cycle of operation is 12 seconds, the time shown in FIG. 2 is 0.0 seconds to 7.25 seconds, and 7.25 seconds.
An operation time of 1 second to 10.05 seconds and 10.05 seconds to 12.0 seconds is allocated.

In the biochemical / immune automatic analyzer according to the present invention, the stirring intensity is adjusted by changing the time for opening the solenoid valve 5 at the position shown in FIG.
That is, when the solenoid valve 5 is opened at the position shown in FIG. 1A, the amount of compressed air according to the time when the solenoid valve 5 is opened is changed to a line from the solenoid valve 5 to a point connected to the reaction cell. It is stored. Therefore, the reaction cell and the air reservoir line are connected at the timing when the position shown in FIG. 1B is reached, and the stored air is supplied to the reaction cell and the reaction solution is stirred.

FIG. 3 is a diagram showing a configuration example of a control system of the solenoid valve.
FIG. 4 is a diagram for explaining the flow of processing of the controller that controls the solenoid valve. In FIG. 3, the solenoid valve 5 is connected to the compressed air line shown in FIG.
The controller 7 adjusts the amount of compressed air to be supplied for stirring up to 1C, and is opened and closed by a driver 6, and a controller 7 controls the driver 6 according to the stirring intensity. In the controller 7, for example, stirring conditions are set for each analysis item from a plurality of stirring conditions (electromagnetic valve operating parameters) in the analysis condition table 71 in advance, and the analysis items are referred to by referring to the analysis condition table 71 when performing analysis. The driver 6 is controlled to open and close the electromagnetic valve 5 under the corresponding stirring conditions. As the solenoid valve operation parameters, for example, the start time, the number of operations, the ON time, and the like are set in the analysis condition table 71.
The start time is the elapsed time after the start of one cycle of operation, the number of operations is the number of times the solenoid valve is opened and closed, and the on time is the time during which the solenoid valve is open. Therefore,
In FIG. 2, when the solenoid valve is opened and closed at the position shown in FIG. 1A, the activation time is set within 7.25 seconds, and the operable time of the solenoid valve at this position is divided by the number of operations ( The time (equally divided) is defined as one operation time, and the ON time is set according to the time width. The stirring intensity is adjusted by the number of operations and the ON time.

Next, the processing flow of the controller will be described. First, when one-step movement of the rotary reactor (VRRV) is started (step S11), the solenoid valve operation parameters are set by referring to the analysis condition table from the analysis item (step S12), and the number of times of operation is cleared to 0 (step S12). Step S1
3). Next, the system waits for the activation time of the solenoid valve (step S14), moves to the position shown in FIG. 1 (a), and when a predetermined time has elapsed, opens the solenoid valve for the set ON time (step S15), and operates. The number of times is incremented by one (step S16). Then, it is checked whether or not the number of operations has reached a predetermined number (step S17). If the number of operations has not reached the predetermined number, the operation waits for a time obtained by subtracting the ON setting time from one operation time (step S18). , Step S
Returning to step 15, the same processing is repeatedly executed until the number of operations reaches a predetermined number.

It should be noted that the present invention is not limited to the above-described embodiment, and various modifications are possible. For example, in the above-described embodiment, the line from the solenoid valve to the air supply point is used as an air reservoir, and air is temporarily stored here and then supplied, but the solenoid valve 5 is opened at the position shown in FIG. A compressed air line may be connected to the reaction cell, and the compressed air may be supplied to the reaction cell as it is in proportion to the opening time of the solenoid valve 5 to stir the reaction solution, or both stirring controls may be used together. May be. In the case where the air is temporarily stored in the line and stirring is performed, relatively weak stirring can be controlled. On the other hand, in the case where the compressed air line is connected to the reaction cell via an electromagnetic valve and the stirring is performed, strong stirring can be controlled. .

[0017]

As is clear from the above description, according to the present invention, since the air is supplied by controlling the opening and closing of the solenoid valve from the compressed air line and the stirring intensity is controlled, a plurality of stirring conditions can be analyzed. Can be selected according to the item. Moreover,
By temporarily storing the air for stirring in the air reservoir and then using the air for stirring, the stirring intensity can be adjusted by the air supply time to the air reservoir, and the stirring intensity can be easily controlled.

[Brief description of the drawings]

FIG. 1 shows an automatic biochemical / immune analyzer according to the present invention.
It is a figure for explaining an example.

FIG. 2 is a diagram for explaining the operation and timing of the automatic biochemical / immune analyzer according to the present invention.

FIG. 3 is a diagram illustrating a configuration example of a control system of a solenoid valve.

FIG. 4 is a diagram for explaining a processing flow of a controller that controls the solenoid valve.

FIG. 5 is a diagram showing an outline of a system configuration of a conventional biochemical analyzer.

[Explanation of symbols]

1A to 1C: reaction cell, 2: rotor of rotary reactor, 3 ...
Rotary reactor stator, 4 ... capillary, 5 ... solenoid valve

────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Miyoshi Tanabe 3-1-2 Musashino, Akishima-shi, Tokyo Japan Electronics Co., Ltd. (56) References JP-A-4-160366 (JP, A) (58) Survey Field (Int.Cl. ⁷ , DB name) G01N 35/00-35/10

Claims (2)

(57) [Claims] 1. A biochemical / immuno-automatic analyzer for dispensing a reagent and a sample into a reaction cell while rotating a rotary reactor and agitating and reacting to analyze the sample is connected to the reaction cell at a stirring position. A compressed air line, and an electromagnetic valve for adjusting the amount of air supplied to the reaction cell from the compressed air line, wherein the stirring intensity is adjusted by adjusting the supply amount of air by opening and closing time of the electromagnetic valve. Biochemical / immune automatic analyzer characterized by the following. 2. A structure in which air is temporarily stored in an air reservoir of a line from a solenoid valve to a reaction cell before the compressed air line is connected to the reaction cell, and stirring is performed by supplying the stored air. 2. The automatic biochemical / immune analyzer according to claim 1, wherein: