JPH09257796A - Analyser and analyzing method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To contrive accurate quantitative analysis by measuring the quantity of the transmitted light passing through a reaction container in analysis using magnetic particles to confirm the re-dispertion of manetic particles when a reagent containing a labelling substance is injected into the reaction container and detecting the residual liquid after suction in a magnetic particle washing process. SOLUTION: A first reagent containing magnetic particles and an analytical sample are injected into a reaction container. A magnet is set after a predetermined time to attract magnetic particles to the inner wall surface of the reaction container by magnetic force and the magnetic particles are washed by a magnetic particle washing mechanism 10. After washing, it is detected whether a washing soln. remains by a transmitted light quantity measuring device 19. If there is no washing soln., the magnet is reset and a second reagent containing a lagelling substance is injected to stirr the reaction container and the re- dispersion of magnetic particles is confirmed by the device 19. The magnet is set after a predetermined time to perform washing in the same way. After it is confirmed that no washing soln. remains by the device 19, a component to be analyzed is detected by a labelling substance detection mechanism 11.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an analyzer and an analytical method used when analytically testing a sample such as serum, and more particularly to an analytical device and an analytical instrument for quantitatively analyzing an analyte in a sample using magnetic particles. Regarding the method.

[0002]

2. Description of the Related Art As a method for analytically inspecting a sample such as serum, a method for quantitatively analyzing an analyte in a sample using magnetic particles has been known. This analysis method has a diameter of about 0.2 to 0.8 microns, preferably 0.4 to 0.6.
An insoluble carrier (hereinafter referred to as magnetic particles) in which a substance that causes an immune reaction (antigen-antibody reaction) with an analyte component in a sample is immobilized on magnetic particles of the order of microns is used for a certain period of time. React. After that, the magnetic particles are washed with a washing liquid to perform B / F separation (separate those bound to the magnetic particles and those not bound). At this time, a magnetic force is applied from the outside of the reaction container to collect the magnetic particles in one place in the reaction container so that the magnetic particles do not flow out of the reaction container during washing. Then, after this, the component to be analyzed which has reacted with the magnetic particles is reacted with an antibody or an antigen, a part of which has been labeled with an enzyme, a fluorescent substance or a chemiluminescent substance, and B / F separation is carried out again after a certain period of time. After that, a reaction depending on the labeled substance is performed, and the generated color development, fluorescence, and chemiluminescence are detected by the respective detection systems.

Thus, the reaction between the magnetic particles and the sample in the first step, the reaction between the immune complex on the magnetic particles and the labeled antibody or antigen in the second step, and so on.
The method of carrying out the reaction in stages is called a two-step sandwich method, and it is necessary to perform B / F separation twice during the process.

Further, the labeled antibody or antigen in the above is different from the antibody or antigen immobilized on magnetic particles in the site that reacts with the component to be analyzed in the sample, and does not react with the immobilized carrier or antigen. There is a method that does not require a second B / F separation using an antibody or an antigen, which is called a one-step sandwich method.

A conventional analyzer for automatically quantitatively analyzing a component to be analyzed in a sample using such an analysis method causes an immune reaction with the component to be analyzed in the sample in a translucent reaction container. A first reagent dispensing mechanism that dispenses magnetic particles as a first reagent, and the target that has washed the magnetic particles dispensed from the first reagent dispensing mechanism into a reaction container and reacted with the magnetic particles. A magnetic particle washing mechanism for removing components other than analytical components from the reaction vessel and a stirring mechanism for stirring the inside of the reaction vessel are provided, and when the washing of the magnetic particles is completed, the reaction is performed from the second reagent dispensing mechanism. The second reagent containing the labeling substance is dispensed into the container. Then, when a predetermined time has elapsed after the second reagent was dispensed into the reaction container, the labeled substance that reacted with the analyte was detected by an optical or chemical method to quantitatively analyze the analyte. It is configured.

By the way, in such a conventional analyzer, if the magnetic particles are dispersed in the reaction container when the test liquid in the reaction container is sucked, the magnetic particles that have reacted with the component to be analyzed are together with the test liquid. It will be sucked and it will not be possible to obtain accurate test results. Therefore, conventionally, when aspirating the test liquid in the reaction container, magnets as magnetic reagent particle adsorbing means are set on both sides of the reaction container, and by adsorbing the magnetic particles to the inner surface of the reaction container with these magnets,
The magnetic particles that have reacted with the component to be analyzed are prevented from being sucked together with the test liquid. When the aspiration of the test solution is completed, the magnet is offset from the set position to the reset position by the magnet offset mechanism so that the magnetic particles are dispersed in the reaction vessel again, and after the second reagent is dispensed, the reaction is performed. The inside of the container was being stirred.

[0007]

However, in the above-described conventional analyzer, the magnet / offset mechanism does not work after the test solution in the reaction container is sucked in the magnetic particle washing step, or the stirring mechanism operates. If not performed, the magnetic particles remain adsorbed on the inner surface of the reaction container, so that even if the second reagent is dispensed into the reaction container in the next step, it is still contained in the second reagent. The reaction between the labeling substance and the component to be analyzed is not promoted, which may adversely affect the test result.

The present invention has been made in view of the above-mentioned problems, and its purpose is to determine whether magnetic particles are redispersed in a reaction container when a reagent containing a labeling substance is dispensed into the reaction container. It is intended to provide an analyzer that can be confirmed and thereby can accurately and quantitatively analyze an analyte component in a sample. Further, the present invention aims to provide an analysis method capable of accurately quantitatively analyzing an analyte component in a sample.

[0009]

The invention according to claim 1 is
In order to solve the above-mentioned problems, a test liquid in which an antigen-antibody reaction has occurred with a first liquid containing at least a sample and fine particles is
/ F is separated, and the second liquid is added to suspend the fine particles, and then the reaction or measurement is performed. In an analyzer, immediately after the addition of the second liquid or after a certain period of time, Suspension measuring means for measuring the degree of suspension, and a control means for judging the suitability of the analytical work based on the output of this suspension measuring means, at an appropriate time depending on the required reaction or measurement time. It is characterized by determining whether or not the fine particles are redispersed in the reaction container.

According to a second aspect of the present invention, in the first aspect of the invention, a test solution suction mechanism section for sucking the test solution in the reaction container and a cleaning solution dispensing mechanism for dispensing the cleaning solution in the reaction container. And a control unit for determining whether or not the test solution remains in the reaction container based on the output of the suspension measuring unit. .

According to a third aspect of the present invention, a test liquid in which an antigen-antibody reaction is caused by the first liquid containing at least the sample and the fine particles is subjected to B / F separation, and the second liquid is added to suspend the fine particles. In an analytical method in which the reaction or measurement is performed after
Immediately after the addition of the second liquid or after a certain period of time, a step of measuring the suspension degree of particles in the reaction vessel and a comparison between the output of the suspension degree measuring step and a set value are performed to judge the suitability of the analysis work. It is characterized in that it is determined whether or not the fine particles are redispersed in the reaction container at an appropriate time point according to a required reaction or measurement time.

[0012]

FIG. 1 is a block diagram showing an embodiment of the present invention.
This will be described with reference to FIG. FIG. 2 is a plan view of an analyzer according to an embodiment of the present invention. As shown in FIG. 2, a circular reaction table 2 is provided on a base 1 of the analyzer according to an embodiment of the present invention. Is rotatably provided.

The reaction table 2 is made of a metal material such as iron, and a plurality of reaction container holding holes 3 are provided at a peripheral portion of the reaction table 2 at regular intervals along the circumferential direction of the reaction table 2. Has been drilled. These reaction container holding holes 3 are for arranging a plurality of reaction containers at a constant pitch in the peripheral portion of the reaction table 2, and the reaction containers inserted and held in these reaction container holding holes 3 are translucent. It is formed of a material having (for example, glass, acrylic resin, etc.).

A motor 4 as a table driving means is installed on the upper surface of the base 1. This motor 4 drives the reaction table 2 to rotate at a constant pitch.
A timing belt 6 is stretched between a drive gear 5 attached to the rotary shaft 4 a of the motor 4 and a ring-shaped driven gear (not shown) attached to the lower surface of the reaction table 2.

Further, in FIG. 2, 7 is a sample dispensing mechanism for dispensing a sample into the reaction container held and inserted in the reaction container holding hole 3, and 8 is a reaction container similarly inserted and held in the reaction container holding hole 3. A first reagent dispensing mechanism for dispensing, as a first reagent, magnetic particles that cause an immunological reaction with a component to be analyzed in a sample, and 9 denotes a component to be analyzed in a reaction container that is also inserted and held in a reaction container holding hole 3. The second reagent dispensing mechanism 10 dispenses a labeling substance that causes an immune reaction as a second reagent, and 10 is an excess for removing the analyte component that has washed with the magnetic particles dispensed in the reaction container and reacted with the magnetic particles. A magnetic particle cleaning mechanism for removing components from the reaction container, 28 is a stirring mechanism for stirring the inside of the reaction container, and the magnetic particle cleaning mechanism 10 is, as shown in FIG.
It comprises a test solution suction mechanism section 10a for sucking the test solution in the reaction vessel and a wash solution dispensing mechanism section 10b for dispensing the wash solution in the reaction vessel.

Reference numeral 11 in FIG. 2 denotes a labeling substance detection mechanism for optically detecting a labeling substance that has reacted with the analyte (in this case, a substance that causes an immune reaction with the analyte is immobilized on a fluorescent substance). And the labeling substance detection mechanism 11 is
For example, it is composed of a light source section for irradiating the reaction container with excitation light, and a fluorescence intensity measuring section provided at a position opposed to the light source section with the reaction container in between.

FIG. 3 is a sectional view taken along the line AA in FIG. 2. As shown in FIG. 3, the reaction container 12 inserted into and held in the reaction container holding hole 3 of the reaction table 2 is located on both sides of the reaction container 12. Magnet holders 13 are provided between the reaction vessels 12 and the reaction vessels 12. These magnet holders 13
As shown in FIG. 4, the magnet holder 1 is supported by a fixed block 14 fixed to the lower surface of the reaction table 2 via a pivot shaft 15 so as to be vertically rotatable.
A magnet 16 serving as a magnetic reagent particle adsorbing means is held at the tip of the magnet 3.

The magnet 16 is for adsorbing magnetic particles to the inner wall surface of the reaction container, and the magnet 16 is provided by a magnet setting mechanism 17 (see FIG. 2) provided above the reaction table 2 as shown in FIG. A magnet reset provided from the position indicated by the solid line (hereinafter referred to as the reset position) to the position indicated by the chain double-dashed line in FIG. 4 (hereinafter referred to as the set position) and provided below the reaction table 2. The mechanism 18 (see FIG. 2) is designed to offset the set position to the reset position.

Further, reference numeral 19 in FIG. 2 is a transmitted light amount measurement as a suspension degree measuring means for irradiating the reaction container 12 in which the labeling substance is dispensed with light and measuring the transmitted light amount of the light transmitted through the reaction container 12. This device is a device, and the transmitted light amount measuring device 19 includes a light source unit 19 a for irradiating the reaction container 12 with light and a light source unit 19 a.
a and a photometric section 19b provided at positions facing each other with the reaction container 12 interposed therebetween.

The photometric unit 19b includes a light receiving element 20, and the signal output from the light receiving element 20 is amplified by an amplifier 21 as shown in FIG. 1 and further digitalized by an A / D converter (not shown). After being converted into a signal, it is supplied to a CPU (central processing unit) 24 as a control means via the input / output port 22 and the data bus 23a.

The CPU 24 includes the motor 4, the sample dispensing mechanism 7, the first reagent dispensing mechanism 8, the second reagent dispensing mechanism 9, the magnetic particle washing mechanism 10, the stirring mechanism 28, and the labeling substance detection mechanism 11 described above. , The magnet set mechanism 17 and the magnet reset mechanism 18 are driven and controlled according to a predetermined control sequence.
A storage device 25 connected via a data bus 23b and a storage device 25 stores, as a set value, the amount of light transmitted through the reaction container while the magnetic particles are redispersed in the reaction container.

In FIG. 1, reference numeral 26 is a display device and 27 is a keyboard device. FIG. 5 is a flowchart for explaining the control sequence of the CPU 24.
The operation of the embodiment of the present invention will be described with reference to this drawing. As shown in FIG. 5, when the reaction container 12 is inserted and held in the reaction container holding hole 3 of the reaction table 2 and an inspection start command is input from the keyboard device 27 to the CPU 24,
The first reagent dispensing mechanism 8 is controlled by the control signal from the CPU 24.
Is activated, whereby the first sample dispensing mechanism 8 dispenses the first reagent containing magnetic particles into the reaction container 12 (step ST1). Then, when the first reagent is dispensed into the reaction container 12, the sample dispensing mechanism 7 is activated by the control signal from the CPU 24, whereby the sample is dispensed from the sample dispensing mechanism 7 into the reaction container 12. (Step ST2).

When a predetermined time has elapsed after the sample was dispensed from the sample dispensing mechanism 7 into the reaction container 12, the CPU 24 operates the magnet setting mechanism 17 (step ST
3). As a result, the magnet 16 is set from the reset position to the set position, and the magnetic force of the magnet 16 causes the magnetic particles contained in the first reagent to be adsorbed to the inner wall surface of the reaction container 12.

When the magnet 16 is set from the reset position to the set position, the CPU 24 operates the test liquid suction mechanism section 10a of the magnetic particle cleaning mechanism 10 (step S).
T4). As a result, when the test liquid dispensed into the reaction container 12 is removed by suction from the reaction container 12 excluding the magnetic particles, and the test liquid excluding the magnetic particles is removed by suction from the reaction container 12, a control signal from the CPU 24 Thus, the cleaning liquid dispensing mechanism unit 10b of the magnetic particle cleaning mechanism 10 is activated, and the cleaning liquid is dispensed from the cleaning liquid dispensing mechanism unit 10b into the reaction container 12 (step ST5). Then, when a predetermined time elapses after the cleaning liquid is dispensed into the reaction container 12, the test liquid suction mechanism unit 1 of the magnetic particle cleaning mechanism 10 is controlled by a control signal from the CPU 24.
0a is activated, and the cleaning liquid dispensed into the reaction container 12 is sucked by the test liquid suction mechanism 10a (step ST
6).

When the cleaning liquid dispensed into the reaction container 12 is sucked by the test liquid suction mechanism portion 10a, the light source portion 19a of the transmitted light amount measuring device 19 is turned on by the control signal from the CPU 24, and the reaction container is fed from the light source portion 19a. 12 is irradiated with light (step ST7). At this time, the light emitted from the light source unit 19a to the reaction container 12 passes through the reaction container 12 and enters the light receiving element 20 of the photometric unit 19b.
The transmitted light signal is output from 0 (step ST
8).

The transmitted light signal output from the light receiving element 20 is amplified by the amplifier 21 and then supplied to the CPU 24.
At this time, the CPU 24 compares the voltage level of the transmitted light signal output from the light receiving element 20 with the set value 1 (the transmitted light amount when the cleaning liquid is not contained in the reaction container 12) stored in the storage device 25 (step ST9). ).

Here, when the voltage level of the transmitted light signal is higher than the set value 1 stored in the storage device 25, CP
U24 determines that the cleaning liquid remains in the reaction container 12. That is, when the cleaning liquid does not remain in the reaction container 12, the amount of transmitted light that passes through the reaction container 12 decreases, so that the cleaning liquid dispensed into the reaction container 12 is sucked by the test liquid suction mechanism 10a and then the reaction container 12 is sucked. It is possible to detect whether or not the cleaning liquid remains in the reaction container 12 by measuring the amount of transmitted light that passes through the inside and comparing the photometric value with the set value stored in the storage device 25.

When it is determined that the cleaning liquid remains in the reaction container 12, the CPU 24 displays on the display device 26 that the cleaning of the magnetic particles performed in the corresponding reaction container 12 is insufficient (step ST24). ).

If the voltage level of the transmitted light signal output from the light receiving element 20 is lower than the set value, the CPU
24 determines that no cleaning liquid remains in the reaction container 12, and then activates the magnet reset mechanism 18 (step ST10). As a result, the magnet 16 is offset from the set position to the reset position, and the magnetic force of the magnet 16 causes the magnetic particles adsorbed on the inner wall surface of the reaction container 12 to start to be released into the reaction container 12.

When the magnet 16 is offset from the set position to the reset position, the second reagent dispensing mechanism 9 is activated by the control signal from the CPU 24, and the second reagent dispensing mechanism 9 causes the labeling substance to enter the reaction container 12. The second reagent containing is dispensed (step ST11). Then, when the second reagent is dispensed into the reaction container 12, the CPU 24 operates the stirring mechanism 28 (step ST12). As a result, the inside of the reaction container 12 is stirred by the stirring mechanism 28, and when the stirring process by the stirring mechanism 28 is completed, the light source unit 19a of the transmitted light amount measuring device 19 is turned on by the control signal from the CPU 24, and the light from the light source unit 19a is emitted. The reaction container 12 is irradiated (step ST13). At this time, the light emitted from the light source unit 19a to the reaction container 12 passes through the reaction container 12 and enters the light receiving element 20 of the photometric unit 19b, and is output as a transmitted light signal from the light receiving element 20 (step ST
14).

The transmitted light signal output from the light receiving element 20 is amplified by the amplifier 21 and then supplied to the CPU 24.
At this time, the CPU 24 compares the voltage level of the transmitted light signal output from the light receiving element 20 with a set value 2 (a photometric value in a state where magnetic particles are not dispersed in the reaction container) stored in the storage device 25. (Step ST15).

When the voltage level of the transmitted light signal output from the light receiving element 20 is larger than the set value 2 stored in the storage device 25, the CPU 24 causes the magnetic particles to be redispersed in the reaction container 12. It is determined not to. That is, when the second reagent is dispensed into the reaction container 12 and stirred, the magnetic particles are not uniformly dispersed in the reaction container 12 and the reaction container 12
If the second reagent is dispensed into the reaction container 12, the amount of transmitted light that passes through the reaction container 12 increases if it remains collected near the inner wall of the reaction container 12. The amount of transmitted light is measured, and the photometric value is stored in the storage device 2
It is possible to detect whether or not the magnetic particles are redispersed in the reaction container 12 by comparing with the set value 2 stored in 5.

When it is determined that the magnetic particles are not redispersed in the reaction vessel 12, the CPU 24 displays on the display device 26 that the stirring by the stirring mechanism 28 is insufficient (step ST24).

Further, the voltage level of the transmitted light signal output from the light receiving element 20 is the set value 2 stored in the storage device 25.
If it is smaller, the CPU 24 determines that the magnetic particles are redispersed in the reaction container 12, and when a predetermined time has passed, the magnet setting mechanism 17 is operated next (step ST16). As a result, the magnet 16 is set from the reset position to the set position, and the magnetic force of the magnet 16 attracts the magnetic particles to the inner wall surface of the reaction container 12.

When the magnet 16 is set from the reset position to the set position, the CPU 24 operates the test liquid suction mechanism section 10a of the magnetic particle cleaning mechanism 10 (step S).
T17). As a result, the labeling substance dispensed into the reaction container 12 is removed except for the labeling substance reacted with the analyte.
When the labeling substance other than the labeling substance that has reacted with the analyte is aspirated and removed from the reaction container 12, the cleaning liquid dispensing mechanism unit 10b of the magnetic particle cleaning mechanism 10 is controlled by the control signal from the CPU 24. The cleaning liquid is dispensed into the reaction container 12 from the cleaning liquid dispensing mechanism 10b (step ST18). Then, when a predetermined time has elapsed after the cleaning liquid was dispensed into the reaction container 12, the test liquid suction mechanism section 10a of the magnetic particle cleaning mechanism 10 was activated by the control signal from the CPU 24, and the cleaning liquid dispensed into the reaction container 12 was activated. Is sucked by the test liquid suction mechanism section 10a (step ST1).
9) The cleaning solution dispensed into the reaction container 12 is the test solution suction mechanism section 10
When aspirated by a, the light source unit 19a of the transmitted light amount measuring device 19 is turned on by the control signal from the CPU 24, and the reaction container 12 is irradiated with light from the light source unit 19a (step ST20). At this time, from the light source unit 19a to the reaction container 12
The light radiated on the light is transmitted through the reaction container 12 and the photometric unit 19
It is incident on the light receiving element 20 of b and is output as a transmitted light signal from this light receiving element 20 (step ST21).

The transmitted light signal output from the light receiving element 20 is amplified by the amplifier 21 and then supplied to the CPU 24.
At this time, the CPU 24 compares the voltage level of the transmitted light signal output from the light receiving element 20 with the set value 1 (the amount of transmitted light when the cleaning liquid is not contained in the reaction container 12) stored in the storage device 25 (step ST22). ).

When the voltage level of the transmitted light signal output from the light receiving element 20 is larger than the set value 1 stored in the storage device 25, the CPU 24 determines that the cleaning liquid remains in the reaction container 12. To do. And the reaction container 12
When it is determined that the cleaning liquid remains inside, the CPU 24 displays on the display device 26 that the cleaning of the magnetic particles performed in the corresponding reaction container 12 is insufficient (step ST2).
4).

When the voltage level of the transmitted light signal output from the light receiving element 20 is lower than the set value, the CPU
No. 24 determines that no cleaning liquid remains in the reaction container 12. Then, the CPU 24 operates the labeling substance detection mechanism 11 to perform a quantitative analysis of the component to be analyzed which has reacted with the labeling substance (step ST23).

Therefore, in one embodiment of the present invention, the second
When a second reagent containing a labeling substance is dispensed from the reagent dispensing mechanism 9 into the reaction container 12, whether or not magnetic particles are redispersed in the reaction container 12 is detected by a signal from the light receiving element 20. Since the quantitative analysis of the component to be analyzed is performed only when it is determined that the magnetic reagent particles are redispersed in the reaction container 12, the component to be analyzed in the sample can be accurately quantitatively analyzed.

In the embodiment of the present invention, the light receiving element 2
When the voltage level of the signal output from 0 is larger than the set value 1 stored in the storage device 25, it is determined that the cleaning liquid remains in the reaction container 12, and therefore the test liquid is diluted by the remaining cleaning liquid. It is possible to eliminate the influence of the error or the B / F separation on the analysis result.

In the above-described embodiment of the present invention, if the magnetic particles are not redispersed in the reaction vessel 12, the CPU 2
When it is judged by No. 4, the fact is displayed on the display device 26. However, the fact that the magnetic particles are not redispersed in the reaction container 12 may be output from the printer, or the magnetic particles may not react. CPU2 if not re-dispersed in container 12
You may make it notify by buzzer when 4 determines.

In the above-described embodiment, the stirring mechanism 28 is used for stirring in the reaction vessel, but the stirring mechanism 2
If the stirring can be performed without using 8, the same effect as in the case of using the stirring mechanism 28 can be obtained.

In the above-described embodiment of the present invention, the amount of transmitted light when the cleaning liquid is not contained in the reaction container 12 is stored in the storage device 25 in advance as the first set value 1. Measure the amount of transmitted light before dispensing the first reagent and sample to 12 and set the measured value to the first set value 1
Alternatively, it may be stored in the storage device 25.

Further, in the above-described one embodiment of the present invention, the amount of transmitted light in the state where the magnetic particles are dispersed in the reaction container is stored in advance in the storage device 25 as the second set value 2. For example, the amount of transmitted light when only the first reagent is dispensed into the reaction container 12 is measured, and the measured value + α (a series of B /
The plus amount of the amount of transmitted light in case of a slight outflow during the F separation operation) may be stored in the storage device 25 as the second set value 2. However, in this case, since the set value is set to the state opposite to the set value in the embodiment of the present invention, the comparison determination in step ST5 of FIG. 5 is reversed.

In the above-described one embodiment of the present invention, the case where the present invention is applied to the analyzer for quantitatively analyzing the component to be analyzed in the sample on the basis of the analysis principle using the fluorescent substance as the labeling substance has been described. Is not limited to
Any analysis that performs redispersion can be applied. For example, an analyzer using a substance different from the labeling substance used in the above-described embodiment (Japanese Patent Laid-Open No. 53-1459).
No. 12, JP-A-47-18597, JP-A-4
No. 58157), and an analyzer for quantitatively analyzing the component to be analyzed in the sample using a substance that emits light or develops a color as a labeling substance (Japanese Patent Laid-Open No. 60-15965).
No. 1), and is also applied to an analyzer having an analysis principle different from that of the above-described embodiment (see JP-A-52-15815, JP-B-6-5989, and JP-A-6-160401). can do.

In the above-mentioned one embodiment, the resuspension is carried out for the reaction, but it is needless to say that the present invention can be applied to the case where the resuspension is carried out for measuring the reaction result. .

Further, in the above-mentioned one embodiment, the analysis by the sandwich method was mentioned, but it is easy to appropriately change the competitive method. Further, in the case of chemiluminescence, the constitution shown in FIGS. 1 to 4 can be applied as it is, and in the case of colorimetric photometry, it is possible to confirm the remaining cleaning liquid and disperse the magnetic particles by the above-mentioned transmitted light amount measuring device 19. It can also be used to confirm whether or not it has been used.

[0048]

As described above, according to the first aspect of the present invention, it is possible to confirm whether or not the fine particles are redispersed in the reaction vessel, and to accurately quantify the component to be analyzed in the sample. An analyzer capable of analyzing can be provided.

According to the invention of claim 2, in addition to the effect of the invention of claim 1, it is possible to confirm whether or not the cleaning liquid remains in the reaction vessel, and it is possible to eliminate the influence of the cleaning liquid. According to the invention of Item 3, it is possible to provide an analysis method capable of accurately and quantitatively analyzing an analyte component in a sample.

[Brief description of drawings]

FIG. 1 is a block configuration diagram of an analyzer according to an embodiment of the present invention.

FIG. 2 is a plan view of the analyzer according to the same embodiment.

FIG. 3 is a sectional view taken along the line AA of FIG. 2;

FIG. 4 is a sectional view taken along the line BB of FIG. 3;

FIG. 5 is a flowchart for explaining the operation of the analyzer according to the embodiment of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Reaction table 4 ... Motor 7 ... Sample dispensing mechanism 8 ... 1st reagent dispensing mechanism 9 ... 2nd reagent dispensing mechanism 10 ... Magnetic particle washing mechanism 10a ... Test liquid suction mechanism part 10b ... Washing liquid dispensing mechanism Part 28 ... Stirring mechanism 16 ... Magnet 17 ... Magnet setting mechanism 18 ... Magnet reset mechanism 19 ... Transmitted light amount measuring device 19a ... Light source part 19b ... Photometric part 24 ... CPU 25 ... Storage device 26 ... Display device

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI technical display location G01N 35/02 G01N 35/02 Z

Claims (3)

[Claims] 1. A test liquid in which an antigen-antibody reaction has occurred with a first liquid containing at least a sample and fine particles is subjected to B / F separation, and a second liquid is added to suspend the fine particles, followed by reaction or measurement. In the analyzer for performing, the suspension degree measuring means for measuring the suspension degree of the particles in the reaction container immediately after the addition of the second liquid or after a fixed time, and the analysis based on the output of the suspension degree measuring means An analyzer comprising: a control means for judging the suitability of the work, and judging whether or not the fine particles are redispersed in the reaction container at an appropriate time point according to a required reaction or measurement time. . 2. A test solution suction mechanism section for sucking a test solution in the reaction vessel, and a cleaning solution dispensing mechanism section for dispensing a cleaning solution in the reaction vessel, wherein the control means comprises: The analyzer according to claim 1, wherein it is determined whether or not the test solution remains in the reaction container based on the output of the suspension measuring means. 3. A test liquid in which an antigen-antibody reaction has occurred with a first liquid containing at least a sample and fine particles is subjected to B / F separation, and a second liquid is added to suspend the fine particles, followed by reaction or measurement. In the analysis method of performing the step of measuring the degree of suspension of particles in the reaction vessel immediately after the addition of the second liquid or after a fixed time, comparing the output of the step of measuring the degree of suspension with a set value. And a step of making a determination as to whether or not the analysis work is appropriate, and determining whether or not the fine particles are redispersed in the reaction container at an appropriate time point according to the required reaction or measurement time. .