JPH08271520A - Automatic biochemical immunological analyzer - Google Patents

Abstract

PURPOSE: To select and regulate the stirring power depending on the item to be analyzed. CONSTITUTION: The automatic analyzer comprises a compressed air line 4 coupled with reaction cells 1A-1C at a stirring position, and a solenoid valve 5 for regulating the quantity of air to be fed to the reaction cells 1A-1C from the compressed air line 4 wherein the air supply is regulated based on the open/close time of the solenoid valve 5 thus regulating the stirring power. Before the compressed air line 4 is coupled with the reaction cells 1A-1C, the air is stored temporarily in an air sump provided in the line between the solenoid valve 5 and the reaction cells 1A-1C, and the stored air is used for stirring. With such structure, the open/close time of the solenoid valve 5 can be preset foe each analytic item and a plurality of stirring conditions can be selected depending on the analytic item. Furthermore, the stirring power can be regulated based on the air supply time to the air sump and thus realizing easy control of the stirring power.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biochemical / immunoautomatic analyzer for analyzing a sample by dispensing a reagent and a sample into a reaction cell while rotating a rotary reactor, stirring and reacting them.

[0002]

2. Description of the Related Art Used in clinical, chemical and pharmaceutical fields,
In a biochemical analyzer that analyzes a sample such as serum or urine, a sample such as serum and a predetermined reagent are mixed in a transparent reaction cell to cause a color reaction, and white light is emitted from a light source. The transmitted light is dispersed by a spectroscope, the absorbance by the dye is determined, and then the concentration of the substance to be measured is determined based on the absorbance.

FIG. 5 is a diagram showing an outline of the system configuration of a conventional biochemical analyzer. 11 is a cup transport unit, 12 is a sample pipette, 13 is a sampling valve, 14 is a switching valve, 15 is a cool box, and 16 is Reagent pump, 17
Is a reagent switching valve, 18 is a rotary reactor, 19 is a detection unit, 20 is an analyzer, and 21 is a data processing unit.

In FIG. 5, a rotary reactor 18 holds 30 to 40 reaction cells on its circumference, and performs cleaning of the reaction cells and measurement of cell blanks while rotating in, for example, 1 step and 12 seconds. Is. For each reaction cell of the rotary reactor 18, first, a certain amount (1 μl to 40 μm) is passed from the cup transport unit 11 through the sample pipette 12.
A sample (specimen) such as serum or urine of about 1 liter) is weighed and guided to the sampling valve 13, from which the first reagent and the sample are dispensed, and the second reagent is also dispensed. In this way, when a sample such as serum and a predetermined reagent are mixed in a transparent reaction cell using the rotary reactor 18 to cause a color reaction, a white light is emitted from the light source in the detection unit 19. The transmitted light is separated by a spectroscope. The measurement data of the detection unit 19 is converted from an analog signal to a digital signal by the analysis unit 20, processed by the data processing unit 21, and the data is stored in the memory, displayed on the CRT, or output from the printer.

By the way, in recent years, in addition to the conventional biochemical measurement items, the following measurement items and reagents requiring stirring strength have begun to be used. These are immunoturbidimetric measurement items, non-adjusted reagents for biochemistry, academic conference-compliant reagents with high substrate concentrations, and the like. In addition, due to the difference in liquid properties due to the concentration of the surfactant and the like, there have been categorized items that result in poor measurement when stirring is increased and items that cannot be measured accurately without increasing stirring.

[0006]

However, the stirring speed and vertical movement of the stirring rod and stirring blade can be controlled by the stirring rod and the stirring blade to achieve optimum stirring according to the measurement item and reaction reagent. There is no biochemical / immunity automatic analyzer equipped with a function that allows selection and control of a plurality of stirring intensities set in advance for each item. Therefore, there is a problem that it is not possible to deal with the measurement items and reagents that require various stirring strengths as described above. Causes problems such as poor stirring and excessive stirring, which makes it impossible to perform highly accurate and precise measurement.

The present invention is to solve the above problems, and an object thereof is to provide an automatic biochemical / immunoanalyzer capable of easily selecting and adjusting the stirring intensity according to the analysis item or the like. To do.

[0008]

To this end, the present invention is a biochemical method for analyzing a sample by dispensing a reagent and a sample into a reaction cell while rotating a rotary reactor and stirring and reacting them.
In the automatic immune analyzer, a compressed air line connected to the reaction cell at the stirring position and a solenoid valve for adjusting the amount of air supplied from the compressed air line to the reaction cell are provided. The stirring strength was adjusted by adjusting the supply amount, and before the compressed air line was connected to the reaction cell, air was once accumulated in the air reservoir of the line from the solenoid valve to the reaction cell, and the accumulation was performed. It is characterized in that it is configured to perform stirring by supplying air.

[0009]

In the biochemical / immunoautomatic analyzer of the present invention, a compressed air line connected to the reaction cell at the stirring position,
An electromagnetic valve for adjusting the amount of air supplied to the reaction cell from the compressed air line is provided, and the stirring intensity is adjusted by adjusting the amount of air supplied by the opening / closing time of the electromagnetic valve. It is possible to preset for each analysis item and select a plurality of stirring conditions according to the analysis item.
Moreover, before the compressed air line is connected to the reaction cell, air is temporarily stored in the air reservoir of the line from the solenoid valve to the reaction cell, and the stored air is used for stirring, whereby the air to the air reservoir is The stirring intensity can be adjusted by the supply time, and the stirring intensity can be easily controlled.

[0010]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a diagram for explaining one embodiment of the biochemical / immunity automatic analyzer according to the present invention, and FIG. 2 is a diagram for explaining the operation / timing of the biochemical / immunity automatic analyzer according to the present invention. Yes, 1A to 1C are reaction cells, 2 is a rotor of a rotary reactor, 3 is a stator of a rotary reactor, 4 is a capillary, and 5 is a solenoid valve.

In FIG. 1, the reaction cells 1A to 1C are held by a rotor 2 of a rotary reactor which moves relative to a stator 3 of the rotary reactor in a direction of an arrow in the drawing as the rotary reactor rotates. The reagent and the sample are further dispensed, and the second reagent is further dispensed. After the first reagent and the sample are dispensed, the second reagent is dispensed at the point and the compressed air is supplied at the point. And stirred. The solenoid valve 5 is connected to a compressed air line and adjusts the amount of compressed air supplied at a point for stirring the reaction cells 1A to 1C, and the stirring intensity is changed depending on the opening / closing time of the solenoid valve 5. It was done like this. The capillary 4 is connected to the line between the solenoid valve 5 and the connection points of the reaction cells 1A to 1C, and the line between them is used as an air reservoir,
The electromagnetic valve 5 is opened and closed at the position shown in FIG. 1 (a) to temporarily store compressed air, which is then supplied to the reaction cell at the position shown in FIG. 1 (b).

The operation of one cycle of the rotary reactor is, for example, from the position of the reaction cell 1A to the position of the reaction cell 1B, and it takes, for example, 12 seconds, and in the middle thereof, FIG. 1 (a) and FIG. Take the position shown in (b).
The position shown in FIG. 1A is a position where the reaction cells 1A to 1C are not connected to the compressed air line of the solenoid valve 5, and the position shown in FIG. 1B is the reaction cells 1A to 1C.
C is a position where the compressed air line of the solenoid valve 5 is connected, and assuming that the operation of one cycle is 12 seconds, the time shown in FIG. 2 is 0.0 seconds to 7.25 seconds, 7.25 seconds.
The operation time is assigned from seconds to 10.05 seconds, 10.05 seconds to 12.0 seconds.

In the biochemical / immunity automatic analyzer according to the present invention, the stirring intensity is adjusted by changing the time for opening the solenoid valve 5 at the position shown in FIG. 1 (a).
That is, when the solenoid valve 5 is opened at the position shown in FIG. 1 (a), the amount of compressed air corresponding to the time when the solenoid valve 5 is opened is changed to a line from the solenoid valve 5 to a point connected to the reaction cell. It can be stored. Therefore, since the reaction cell and the air reservoir line are connected at the timing when the position shown in FIG. 1B is reached, the stored air is supplied to the reaction cell and the reaction liquid is stirred.

FIG. 3 is a diagram showing a configuration example of a control system of a solenoid valve,
FIG. 4 is a diagram for explaining the processing flow of the controller that controls the solenoid valve. In FIG. 3, the solenoid valve 5 is connected to the compressed air line shown in FIG.
The controller 7 adjusts the amount of compressed air to be stirred for stirring up to 1C, and the controller 7 is opened and closed by the driver 6 and controls the driver 6 according to the stirring intensity. In the controller 7, for example, stirring conditions are set in advance in the analysis condition table 71 from a plurality of stirring conditions (solenoid valve operation parameters) for each analysis item, and the analysis condition table 71 is referred to when performing the analysis. The driver 6 is controlled to open and close the solenoid valve 5 under the corresponding stirring conditions. As the solenoid valve operation parameter, for example, the activation time, the number of operations, the on time, etc. are set in the analysis condition table 71.
The start-up time is the elapsed time after the start of one cycle of operation, the number of operations is the number of times the solenoid valve is opened and closed, and the on-time is the time when the solenoid valve is open. Therefore,
In FIG. 2, when the solenoid valve is opened and closed at the position shown in FIG. 1A, the starting time is set within 7.25 seconds and the operable time of the solenoid valve at this position is divided by the number of operations ( The time (equally divided) is set as one operation time, and the ON time is set by the time width. Then, the stirring intensity is adjusted by the number of operations and the ON time.

Next, the processing flow of the controller will be described. First, when the one-step movement of the rotary reactor (VRRRV) is started (step S11), the solenoid valve operation parameter is set by referring to the analysis condition table from the analysis item (step S12), and the number of operations is cleared to 0 ( Step S1
3). Next, it waits until the start time of the solenoid valve (step S14), and when it moves to the position shown in FIG. 1 (a) and reaches a predetermined time, the solenoid valve is opened for the set time of ON (step S15) and the operation is performed. The number of times is incremented by 1 (step S16). Then, it is checked whether or not the number of times of operation reaches a predetermined number of times (step S17). If the number of times of operation has not reached the predetermined number of times, after waiting for a time obtained by subtracting the set time of ON from one operation time (step S18) , Step S
Returning to 15, the same processing is repeatedly executed until the number of operations reaches a predetermined number.

The present invention is not limited to the above embodiment, but various modifications can be made. For example, in the above-described embodiment, the line from the solenoid valve to the air supply point is used as an air reservoir, and air is temporarily stored here before being supplied. However, the solenoid valve 5 is opened at the position shown in FIG. 1 (b). A compressed air line may be connected to the reaction cell, and compressed air may be supplied to the reaction cell as it is in proportion to the opening time of the solenoid valve 5 to stir the reaction liquid, or both stirring controls may be used together. You may. When the air is temporarily stored in the line and agitated, relatively weak agitation can be controlled, whereas when the compressed air line is connected to the reaction cell via an electromagnetic valve and agitated, strong agitation can be controlled. .

[0017]

As is apparent from the above description, according to the present invention, the opening and closing of the electromagnetic valve is controlled from the compressed air line to supply air to control the stirring strength, so that a plurality of stirring conditions can be analyzed. It can be selected according to the item. Moreover,
By temporarily storing the air for agitation in the air reservoir and then using it for agitation, the agitation intensity can be adjusted by the time of supplying the air to the air reservoir, and the agitation intensity can be easily controlled.

[Brief description of drawings]

FIG. 1 is a biochemical / immunoautomatic analyzer 1 according to the present invention.
It is a figure for explaining an example.

FIG. 2 is a diagram for explaining the operation / timing of the biochemical / immunity automatic analyzer according to the present invention.

FIG. 3 is a diagram showing a configuration example of a control system of a solenoid valve.

FIG. 4 is a diagram for explaining a processing flow of a controller that controls an electromagnetic valve.

FIG. 5 is a diagram showing an outline of a system configuration of a conventional biochemical analyzer.

[Explanation of symbols]

1A-1C ... Reaction cell, 2 ... Rotor of rotary reactor, 3 ...
Rotor Reactor Stator, 4 Capillary, 5 Solenoid Valve

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Miyoshi Tanabe 3-1-2 Musashino, Akishima-shi, Tokyo Nippon Electric Co., Ltd.

Claims (2)

[Claims] 1. An automatic biochemical / immunoanalyzer that dispenses a reagent and a sample into a reaction cell while rotating a rotary reactor, stirs and reacts to analyze the sample, and is connected to the reaction cell at a stirring position. A compressed air line and a solenoid valve for adjusting the amount of air supplied from the compressed air line to the reaction cell are provided, and the stirring intensity is adjusted by adjusting the amount of air supplied according to the opening / closing time of the solenoid valve. A biochemical / immunity automatic analyzer characterized in that 2. A structure in which air is temporarily stored in an air reservoir of a line from a solenoid valve to a reaction cell before the compressed air line is connected to the reaction cell, and stirring is performed by supplying the stored air. The biochemical / immunity automatic analyzer according to claim 1, wherein