JP2989676B2 - Decolorization and deodorization of yeast extract extraction residue - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for decolorizing and deodorizing a residue obtained by extracting yeast extract from yeast, which is mainly composed of yeast cell walls. Yeast extract, which is a useful substance extracted from brewer's yeast or other yeast, is extracted from yeast extract containing yeast cell walls as a main component, and can be used in various fields by purifying, decolorizing and deodorizing. According to the method of the present invention, since there is no possibility that the medicine remains, the method of the present invention is particularly effective in preventing the oxidation of foods, promoting the preservation of fragrance, preventing and improving the deterioration of aroma, or preventing the deterioration of foods and promoting storage. Can be used effectively.

[0002]

2. Description of the Related Art As a method for decolorizing and deodorizing a yeast extract extraction residue, for example, an alkali such as caustic soda and hydrogen peroxide as a bleaching agent are mixed with the yeast extract extraction residue and heated. Methods are known. However, decolorization by chemicals such as hydrogen peroxide
The deodorizing method is harmful due to the remaining of these chemicals, and has a problem in terms of safety for use in foods and the like, and also has a problem of oxidized odor and an insufficient oil adsorbing ability. .

[0003] The present invention solves these conventional drawbacks, has no harm due to residual medicine, has no problem in terms of safety in use in foods and the like, has no oxidizing odor, and has a low oil adsorption capacity. It is another object of the present invention to provide a method for decolorizing and deodorizing an excellent yeast extract extraction residue.

[0004]

That is, the present invention provides a method for treating a yeast extract, which is obtained by extracting a yeast extract from yeast and having a yeast cell wall as a main component, with an alkali and an acid.
1000-10000 ppm ozone gas concentration
Another object of the present invention is to provide a method for decolorizing and deodorizing a yeast extract extraction residue, which comprises performing ozone treatment for 20 minutes and treating with ethanol once or twice before and after the ozone treatment.

[0005] The yeast extract extraction residue used in the method of the present invention is obtained by extracting yeast extract from brewer's yeast or other yeast, and having a yeast cell wall as a main component. The extraction residue of the yeast extract may be dried after the extraction, or may be immediately after the extraction.

Next, in the method of the present invention, the yeast extract extraction residue as described above is treated with an alkali and an acid. Here, the alkali treatment may be performed using, for example, a sodium hydroxide solution of about 0.1 to 0.5 N or the like. Usually, the mixture is boiled under reflux for about 60 to 120 minutes after adding about 0.5N sodium hydroxide solution.

In the present invention, usually, after the alkali treatment, centrifugation and washing with water are performed, followed by acid treatment. Here, the acid treatment may be performed, for example, with a hydrochloric acid solution of about 0.1 to 0.5 N or the like. Usually, the mixture is refluxed and boiled for about 60 to 120 minutes after adding about 0.5N hydrochloric acid solution. Usually, centrifugation and washing are performed after the acid treatment.

As described above, the yeast extract extraction residue is treated with an alkali and an acid, and then treated with ozone. Normally, yeast cell walls and the like after extraction of yeast extract are oxidized and colored, but by focusing on the sterilization, deodorization, and decolorization effects of ozone gas, the yeast extract extraction residue is treated with alkali and acid, and then treated with ozone. Decolorization and deodorization can be performed in a short time.

This ozone treatment depends on conditions such as ozone gas concentration, reaction time, reaction treatment temperature, degree of coloration of the sample, etc., and it is very difficult to determine uniquely. However, it is usually 1000 to 20,000 ppm concentration, preferably 80
Using ozone gas having a concentration of 00 to 12000 ppm, 1
The coloration of organic substances and the like can be decolorized by performing the reaction for 120 to 120 minutes, preferably 3 to 60 minutes.

[0010] Specifically, for example, a sample to be purified and decolorized (residue from extraction of yeast extract) is placed in a glass container, and ozone gas is passed through a diffuser tube or the like, followed by bubbling. The waste ozone gas in the container may be decomposed and exhausted through an ozone killer such as activated carbon.

Further, in the present invention, it is necessary to perform ethanol treatment by adding ethanol once before or after the ozone treatment or twice in total before and after the ozone treatment. Here, if the ethanol treatment is not performed, an unpleasant odor due to the use of ozone may remain, which is not preferable. This ethanol treatment may be performed once or before or after the ozone treatment.
It may be performed many times, and even if it is performed many times (three times or more), not only is the decoloring / deodorizing effect not improved, but rather the oil adsorbing ability is undesirably reduced. Normally, ethanol may be added after the ozone treatment.

As described above, decolorization and deodorization of the yeast extract extraction residue can be performed. Usually, this is further freeze-dried, and the obtained white powder substance is used for various purposes. However, it may be white and mud-like before freeze-drying.

[0013]

Next, the present invention will be described in detail with reference to examples.

EXPERIMENTAL EXAMPLE 1 (Experiment for comparing effects with and without ozone treatment) Dry B yeast 10 which is a yeast extract extraction residue
After adding 1000 ml (milliliter) of water to 0 g,
Ozone treatment was performed for 8 hours using 8000 ppm ozone gas, and then centrifuged at 3000 rpm for 20 minutes.
A white powdery substance obtained by washing with water and freeze-drying was used as a sample (B) of the present invention.

With respect to the obtained sample, the volume ratio and the oil adsorption capacity were measured. The results are shown in Table 1. As a control, Table 1 shows the volume ratio and the oil adsorption capacity of the sample (A) (in the case of using alkali and hydrogen peroxide) in the chemical treatment section together with the sample in the untreated section. In the chemical treatment section, 1000 ml of 0.5N sodium hydroxide solution and 2% hydrogen peroxide were added to 20 g of dry B yeast, which was the extract residue of yeast extract, and the mixture was boiled under reflux for 120 minutes.
Centrifuged at 000 rpm for 20 minutes, washed with water, and then freeze-dried to obtain a powdery substance as sample (A). The volume when the same weight was weighed as a sample was compared, and the volume ratio is shown in Table 1 together with the oil adsorption capacity. The color tone (degree of bleaching) was determined by visual comparison.

[0016]

[Table 1]

The ozone-treated sample (B) had a slight off-flavor (oxidative smell) and was decolorized to almost white, but slightly pinkish. Therefore, in the following experiment, the ozone concentration was set to 10000 ppm, and the treatment was performed for 1 hour.

EXPERIMENTAL EXAMPLE 2 (Comparative experiment of the effect of pre-treatment on dry B yeast) To 20 g of dry B yeast, which is a residue of yeast extract, 1000 ml of 0.5 N sodium hydroxide solution was added, and After boiling under reflux for 1 minute, the mixture was spun down and washed with water at 3000 rpm for 20 minutes. Next, 1000 ml of 0.5N hydrochloric acid solution was added, and the mixture was boiled under reflux for 120 minutes, and then 3000 rpm
For 20 minutes. After performing the above pretreatment, ozone treatment was performed for 1 hour using an ozone gas having a concentration of 10,000 ppm, and then 20 minutes at 3000 rpm.
The sample was centrifuged, washed with water, and then freeze-dried to obtain a sample (L) of the present invention. About the obtained sample, the volume ratio and the oil adsorption capacity were measured. The results are shown in Table 2.

As a control, Table 2 shows the volume ratio and oil adsorption capacity of the following samples (J) and (K) together with the sample (A) (treated with a chemical solution). In addition,
Sample (J) was prepared by adding 2% hydrogen peroxide after the above pretreatment, boiling under reflux for 120 minutes, centrifuging and washing at 3000 rpm for 20 minutes, and then freeze-dried (ozone treatment). None). On the other hand, after the above pretreatment, 2% hydrogen peroxide was added to the sample (K), the mixture was boiled under reflux for 120 minutes, and then subjected to ozone treatment with 10000 ppm ozone gas for 1 hour, followed by 20 minutes at 3000 rpm. It was centrifuged and washed with water for 10 minutes and then freeze-dried.

Experimental Example 3 (Comparative experiment on the effect of pre-treatment on B yeast immediately after extraction of yeast extract)
First, to 20 g (as an anhydride) of the yeast B immediately after extraction of the yeast extract, 1000 ml of a 0.5 N sodium hydroxide solution was added, and the mixture was boiled under reflux for 120 minutes.
The mixture was spun down and washed with water at 0 rpm for 20 minutes. Next, 1000 ml of a 0.5N hydrochloric acid solution was added, and the mixture was boiled under reflux for 120 minutes, and then centrifuged and washed at 3000 rpm for 20 minutes. After performing the above pretreatment, 1000
After ozone treatment with ozone gas of 0 ppm concentration for 1 hour, centrifuged and washed with water at 3000 rpm for 20 minutes,
Subsequently, what was freeze-dried was used as sample (P) of the present invention. About the obtained sample (P), the volume ratio and the oil adsorption capacity were measured. The results are shown in Table 2.

As a control, the following sample (O),
The volume ratio and oil adsorption capacity of (M) and (N)
It is shown in the table. The sample (O) was prepared after the above pretreatment.
After adding 2% hydrogen peroxide and boiling for 120 minutes under reflux,
It was centrifuged at 3000 rpm for 20 minutes, washed with water, and then freeze-dried (no ozone treatment). The sample (M) was the same as the sample (P) except that the sample was directly subjected to ozone treatment without performing the pretreatment as described above. Further, after the above pretreatment, 2% hydrogen peroxide was added to the sample (N), and the mixture was boiled under reflux for 120 minutes.
After ozone treatment for 1 hour using ozone gas having a concentration of 000 ppm, the mixture was centrifuged and washed at 3000 rpm for 20 minutes, and then freeze-dried.

[0022]

[Table 2]

[0023]

[Table 3]

From the results shown in Table 2, the sample of the present invention (L),
(P) was found to be superior to the control sample in volume ratio and oil adsorption capacity. Sample (B) in Table 1
(No pretreatment), the sample of the present invention (L),
(P) has a higher volume ratio than sample (B) (no pretreatment).
It was also found that the oil adsorption ability was excellent.

When the samples (L) and (P) of the present invention were compared, the sample (L) was superior. However, the following experiment 4 was performed because the ozone-treated sample had a slightly unpleasant odor.

Experimental Example 4 (Comparison experiment of effects when pretreatment and ethanol treatment were added) In the preparation method of sample (L) in Experimental Example 2, the same procedure was carried out except that ethanol treatment was further performed. Was. In addition, about the sample of this invention, before ozone treatment (sample (S))
Alternatively, at any time later (sample (R)), 99.
An ethanol treatment was performed by adding 1000 ml of 5% ethanol.

As a control, three times before and after the alkali treatment with sodium hydroxide and before and after the ozone treatment, 9 times
A sample (Q) that had been subjected to an ethanol treatment in which 1000 ml of 9.5% ethanol was added was used.
With respect to the obtained samples (Q), (R), and (S), a sensory test of fragrance and color tone was performed together with a test of a volume ratio and an oil adsorption ability. The results are shown in Table 3. In addition, a sensory test of fragrance and color tone was performed on the sample (A) (which had been treated with a chemical solution) together with a test of a volume ratio and an oil adsorption capacity. The results are shown in Table 3.

[0028]

[Table 4]

From the results shown in Table 3, the sample (R) of the present invention,
It can be seen that (S) is superior to the control sample (A) in terms of volume ratio and oil adsorption capacity, and also in terms of fragrance and color tone. In addition, the samples (R) and (S) of the present invention showed a volume ratio, a volume ratio, which was lower than that of the control sample (Q) which was subjected to ethanol treatment three times before and after the alkali treatment with sodium hydroxide and before and after the ozone treatment. It was found that the oil adsorption ability was excellent.

From the results in Tables 1 to 3, the yeast extract extraction residue was first treated with an alkali and an acid,
Ozone treatment with ozone gas having a concentration of 0 to 20,000 ppm for 1 to 120 minutes and treatment with ethanol once or twice before or after the ozone treatment do not cause harm due to the residual drug, such as food. There is no problem in terms of safety in the use for odor, and there is no oxidation smell,
It was found that a white powdery substance having excellent oil adsorption ability could be obtained.

[0031]

According to the method of the present invention, since ozone is used instead of hydrogen peroxide or the like in the purification treatment of the yeast extract extraction residue, the drug remains in the white powdered purified B yeast. There is no harm from foods, and there is no problem in terms of safety for use in foods. Moreover, according to the method of the present invention,
Decolorization, deodorization, and purification of the yeast extract extraction residue can be performed efficiently, and a purified B yeast having no oxidizing odor and excellent oil adsorption ability can be obtained.

Therefore, the purified B yeast obtained by the method of the present invention can be used as a substitute for a part of the batter of fried foods such as tempura, for example, in the case of foods, to prevent deterioration of the product, It can be used as a substitute for processing (eg, coating), etc. to prevent oxidation of products and deterioration of aroma.
Alternatively, it can be used for flavor improvement.

It can also be used in cosmetics-related applications, such as using it as a base for basic cosmetics or as a base for packs, or as a partial substitute for fat adsorbents or powders.

In addition, pulp and the like are partially substituted and used for paper and the like, used as an adsorbent for adsorbing oil and odor, and used as a wall material by adsorbing fragrance. It is also possible to improve the environment of the room or to use it as a deodorizing or masking material for toilets and the like.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-49-75777 (JP, A) JP-A-56-163657 (JP, A) JP-B-48-15621 (JP, B1) 504191 (JP, A) (58) Field surveyed (Int. Cl. ⁶ , DB name) A23L 1/27-1/28 A23L 1/015 A23L 3/3445

Claims (1)

(57) [Claims] Claims 1. A yeast extract extracted from yeast, a yeast extract extraction residue containing yeast cell walls as a main component, treated with an alkali and an acid, followed by ozone treatment with 1000 to 20,000 ppm ozone gas for 1 to 120 minutes,
A method for decoloring and deodorizing a yeast extract extraction residue, wherein the method is treated with ethanol once or twice before or after the ozone treatment.