JP2902878B2 - Method for producing antioxidant substance - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a process for the preparation of an anti-oxidizing materials for the production of antioxidant from the hot water extract of the waste or smoked fish such discharged in the manufacturing process of cutting the fish section acids.

[0002]

Conventionally, in the manufacture process of the cutting of fish clause class
Then , there is a step of steam sterilizing the fish segments under high temperature and high pressure for the purpose of softening and sterilizing the fish segments to make them easy to cut . The waste liquid discharged from the steam sterilization process is low in concentration, but contains fat, protein, and umami components .
Containing soil of useful substances.

[0003]

SUMMARY OF THE INVENTION
Emissions from the steam sterilization process in the manufacturing process of fish cutlery
Waste liquid is discharged to the outside the plant after having been subjected to processing such as denitrification. In some cases, that have been used in food processing the waste as tone <br/> taste solution.

[0004] The present inventor has manufactured the antioxidant to efficiently separate and recover antioxidant from smoked fish such hot water extract sharpener waste or smoked fish such discharged in the manufacturing process of the Kezuribushi of
It shall be the object of the present invention to provide a method.

[0005]

According to the present invention, there is provided a method for producing an antioxidant substance, comprising the steps of: washing a wastewater or a hot-water extract of a fish segment, which is discharged in a production process of shaving fish segments, with ether; this
After treating the waste liquid or the hot water extract washed with ether with acetone or alcohol to remove proteins,
It is decolorized with activated carbon.

[0006]

According to the method for producing an antioxidant substance of the present invention, the waste liquid discharged in the production process of shaving fish knots or the hot water extract of fish knots is washed with ether to remove fat-soluble substances. Acetone or alcohol- soluble protein
After divided, and suction extraction separation difficult coloring component with activated carbon
By decolorized by wearing removed, efficiently producing antioxidant with sufficient antioxidant activity.

[0007]

EXAMPLE An example of the method for producing an antioxidant of the present invention will be described below.

[0008] As a raw material for the production of antioxidant, bonito, waste liquid discharged by the step of steam sterilizing the fish clause acids under high temperature and high pressure during the process of manufacturing the grinding of smoked fish such Sabanado, Alternatively, a hot water extract of fish fins can be used.

Hereinafter, the waste liquid will be described as a raw material.

First, the waste liquid is mixed with an approximately equivalent amount of ether (for example, diethyl ether, etc.) and stirred vigorously. Then, the upper ether layer is removed, and the waste liquid is washed. This washing removes fat-soluble components such as fats from the waste liquid.

Next, about 9 times the amount of acetone or alcohol (eg, ethanol) is added to the waste liquid to precipitate and remove high molecular compounds such as proteins. Then, acetone or alcohol is distilled off under reduced pressure using a rotary evaporator or the like. Incidentally, the aqueous solution obtained after acetone or alcohol treatment of the waste solution is colored brown.

Next, the colored aqueous solution is brought into contact with activated carbon, and the coloring component is adsorbed on the activated carbon to decolorize the aqueous solution.

The aqueous solution thus obtained contains an antioxidant and can be used as an antioxidant as it is. If necessary, the aqueous solution is dried to form a powder. May be used.

Hereinafter, the present invention will be specifically described with reference to Examples and Test Examples.

[0015] In the process of manufacturing the grinding of Example bonito, it was mixed with effluent 10 liters of diethyl ether 10 l discharged upon steam sterilization of dried bonito under high temperature and high pressure, and stirred vigorously for 15 min.

After this stirring, the upper ether layer was removed. Next, the waste solution to remove the ether layer, the addition of 90 l of acetone and allowed to stand for 24 hours.

After this standing, the supernatant was separated from the precipitate by decantation, and the acetone in the supernatant was distilled off under reduced pressure using a rotary evaporator to obtain 8.5 liters of a brown aqueous solution. Was.

Next, 200 g of activated carbon was added to the aqueous solution, and the mixture was slowly brought into contact with stirring for 3 hours.

[0019] After the stirring, the aqueous solution was filtered and the filtrate was concentrated to approximately 0.5 liters and freeze dried. As a result, 17 g of a powdery pale yellow antioxidant substance was obtained.

Test Example The antioxidant activity of the antioxidant obtained in the above example was measured by the iron-rodan method.

That is, the aqueous solution of the antioxidant obtained in the above example (concentration: 0.01%, 0.02%, 0.03%)
%) To adjust the respective aqueous solutions of these antioxidant 2
Milliliter, 10 ml of 0.02 M linoleic acid aqueous solution, and 0.1 M phosphate buffer (pH 7.0) 8
Ml were mixed respectively, the these mixed solution 40 ° C.
For 7 days each .

A mixture containing no antioxidant as a control and a mixture containing α-tocopherol instead of an antioxidant as a control were similarly prepared and incubated.

[0023] After this incubation, the reaction solution 0.1 ml was collected each minute, this 75% ethanol solution 4.7 ml, 30% rhodanide aqueous ammonium 0.1 ml and 0.02M ferric chloride hydrochloride (3.5%) their aqueous solution 0.1 ml
Each was added and left at room temperature for 3 minutes, and the absorbance at 500 nm was measured. The results are shown in Table 1.

[0024]

[Table 1] As apparent from the results shown in Table 1, the anti-oxidizing substances present invention has antioxidant activity comparable to α- tocopherol.

[0025]

According to the method for producing an antioxidant substance of the present invention, waste liquid or fish discharged in the production process of shaving fish segments
The hot water extract of knots is first washed with ether and
Remove water-soluble substances with acetone or alcohols
After removing the protein that is
To decolorize the pile colored components removed by adsorption, antioxidant efficiency with readily sufficient antioxidant activity by a simple operation
Well obtained, cut with a very effective utilization of the waste. Also,
This antioxidant is obtained from edible natural products and contains no harmful synthetic substances, so that it can be used safely in food processing.

Claims (1)

(57) [Claims] 1. A waste liquid or a hot water extract of a fish segment which is discharged in a manufacturing process of shaving fish segments is washed with ether, and the waste liquid or the hot water extract washed with the ether.
A method for producing an antioxidant substance, which comprises treating protein with acetone or alcohol to remove proteins, and then decolorizing with activated carbon.