JPH06141832A - Production of anti-oxidative substance - Google Patents

Abstract

PURPOSE:To effectively utilize a waste solution, etc., discharged from a shaving process of dried fishes for flakes. CONSTITUTION:A waste solution discharged from a shaving process of dried fishes for flakes or a hot water extract of the dried fishes is washed with ether and then treated with acetone or alcohol to remove protein. The product is decolorized by active carbon and a material having anti-oxidant action is obtained. By employing this simple operation, a material having sufficient anti- oxidant activity is easily manufactured from the waste solution discharged from the shaving process of dried fishes and thus the waste solution is effectively utilized.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing an antioxidant substance, for example, a method for producing an antioxidant substance from a waste liquid discharged in a fish shaving cutting process.

[0002]

2. Description of the Related Art Conventionally, the manufacturing process of shaving fish clauses includes a step of steam sterilizing the fish clauses under high temperature and high pressure for the purpose of sterilizing and softening the fish clauses in order to facilitate sharpening. The waste liquid discharged from this steam sterilization process contains useful substances such as fat, protein, and umami components, although the concentration is low.

[0003]

However, the above waste liquid is discharged to the outside of the factory after being subjected to treatment such as denitrification. In some cases, this waste liquid is used as a seasoning liquid in food processing, but at present, the use of this waste liquid is limited to seasoning liquids, and from this waste liquid, physiologically active substances other than general nutrients such as fats can be found. There is no report that it was launched.

The inventor of the present invention has conducted repeated research to effectively utilize the waste liquid discharged in the manufacturing process of the shaving of fish clause, and as a result, found an antioxidant substance from the waste solution or the fish clause. The present invention has been completed in consideration of an efficient method for separating and recovering antioxidant substances.

[0005]

Means for Solving the Problems The method for producing an antioxidant according to the present invention comprises washing a waste liquid or a hot water extract of fish clauses with ether with a waste solution discharged in a fish shaving production step, After deproteinizing by treating with acetone or alcohol, it is decolorized with activated carbon.

[0006]

In the method for producing an antioxidant substance of the present invention, the waste liquid discharged in the step of producing fish shavings or the hot water extract of fish segment is washed with ether to remove fat-soluble substances, After deproteinizing with acetone or alcohols and decolorizing with activated carbon, an antioxidant substance having sufficient antioxidant activity is produced.

[0007]

EXAMPLES An example of the method for producing an antioxidant of the present invention will be described below.

As a raw material for producing an antioxidant substance, a waste liquid discharged in a process of steam sterilizing fish sections under high temperature and high pressure during a process of producing shavings of fish sections such as bonito and mackerel, Alternatively, a hot water extract of fish section can be used.

The waste liquid will be described below as a raw material.

First, this waste liquid is mixed with an approximately equal amount of ether (such as diethyl ether), and after vigorous stirring, the upper ether layer is removed and the waste liquid is washed. By this washing, fat-soluble components such as fat are removed from the waste liquid.

Next, about 9 times the amount of acetone or alcohol (eg ethanol) is added to this waste liquid to precipitate and remove high molecular compounds such as proteins. Then, acetone or alcohol is distilled off under reduced pressure with a rotary evaporator or the like. The aqueous solution obtained after treating the waste liquid with acetone or alcohol is colored brown.

Next, this colored aqueous solution is brought into contact with activated carbon to adsorb the coloring component to the activated carbon, thereby decolorizing the aqueous solution.

The aqueous solution thus obtained contains an antioxidant substance and can be used as an antioxidant substance as it is. However, if necessary, this aqueous solution is dried to a powder form. You may use it.

The present invention will be specifically described below with reference to examples and test examples.

In the process of manufacturing the dried bonito flakes of the Example, 10 liters of the waste liquid discharged when the bonito flakes were steam sterilized under high temperature and high pressure and 10 liters of diethyl ether were mixed and vigorously stirred for 15 minutes.

After stirring, the upper ether layer was removed. Next, 90 liters of acetone was added to the waste liquid from which the ether layer was removed, and the mixture was left standing for 24 hours.

After standing, the supernatant was separated from the precipitate by decantation, and the acetone in this supernatant was distilled off under reduced pressure with a rotary evaporator to obtain 8.5 liters of a brown aqueous solution.

Next, 200 g of activated carbon was added to this aqueous solution,
The mixture was slowly contacted with stirring for 3 hours.

After stirring, the aqueous solution was filtered, the filtrate was concentrated to about 0.5 liter and freeze-dried. As a result, 17 g of a powdery, pale yellow antioxidant substance was obtained.

Test Example The antioxidative activity of the antioxidative substances obtained in the above Examples was measured by the iron iron method.

That is, the aqueous solutions of the antioxidant substance (concentration: 0.01%, 0.02%, 0.03%) obtained in the above-mentioned examples were adjusted, and 2 ml of this antioxidant substance solution, 0.02
10 ml of M linoleic acid aqueous solution and 8 ml of 0.1 M phosphate buffer (pH 7.0) were mixed, and this mixture was incubated at 40 ° C. for 7 days.

As a control, a mixed solution containing no antioxidant and a control containing α-tocopherol instead of the antioxidant were similarly prepared and incubated.

After the incubation, 0.1 ml of the reaction solution was collected, to which 4.7 ml of 75% ethanol solution, 0.1 ml of 30% aqueous solution of rhodanammonium and 0.1 ml of 0.02M ferric chloride (3.5%) aqueous solution were added, and the mixture was allowed to stand at room temperature. After standing for 3 minutes, the absorbance at 500 nm was measured. The results are shown in Table 1.

[0024]

[Table 1] As is clear from Table 1, the antioxidant of the present invention is α-
It has antioxidant activity comparable to tocopherol.

[0025]

EFFECTS OF THE INVENTION According to the method for producing an antioxidant of the present invention, an antioxidant having sufficient antioxidant activity can be obtained by a simple operation such as extraction operation from waste liquid discharged in the production process of fish shavings. Oxidizing substances can be easily produced, and the waste liquid conventionally discharged outside the factory can be used very effectively. Further, since this antioxidant substance is obtained from edible natural products and does not contain any harmful synthetic substance, it can be safely used for food processing.

Claims (1)

[Claims] 1. A waste liquid or hot water extract of a fish clause discharged from a fish shaving production process is washed with ether and treated with acetone or alcohol to remove proteins,
A method for producing an antioxidant, which comprises decolorizing with activated carbon.