JP2774882B2 - Whitening and anti-aging cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening and anti-aging cosmetic which has a whitening effect, smoothes the skin and keeps youthful skin.

[0002]

BACKGROUND OF THE INVENTION agrimony genus dicots network, Hanareben flower Amo, belongs to the rose family. In Japan, Agrimony (Agrimo
nia eupatoria) grows natively in Honshu, Shikoku, Kyushu and Ryukyu,
It is a perennial plant that can be found on mountains and roadsides. Outside Japan, it is distributed in the warm to temperate zones of Korea, Taiwan, Siberia, and the Himalayas.

[0003] This grass is called Senkaku-so and the whole grass is used. It is used for hemostasis, antidiarrhea, antiphlogistic, tonic, and for various bleeding symptoms such as hematemesis, bloody stool, hematuria, cramps, and dysentery. However, as a cosmetic, it is only used as a head cosmetic in JP-A-1-313414 and as a bactericidal composition in JP-A-60-184024.

[0004]

SUMMARY OF THE INVENTION The object of the present invention is to provide a skin-whitening and aging preservation of youthful skin from a readily available raw material, which is safe and smooth when applied to the skin, has a skin-whitening effect, and keeps youthful. An object of the present invention is to provide a preventive cosmetic.

[0005]

The inventors of the present invention have conducted tests on a number of plant extracts that are easily available, and as a result, it has been found that Sengakurusa is very useful as a cosmetic in terms of its whitening effect and skin aging prevention effect. The present invention was found to be effective, and the present invention was completed. That is, the present invention is a whitening and anti-aging cosmetic comprising a solvent extract of a whole plant belonging to the genus Rosewood.

The genus Agaricus is already sold as a crude drug, its dried whole plant is sold as a raw material for a medicinal product, and it is possible to easily collect the native one in Yamano, Japan. To make a cosmetic raw material, it is extracted with a hydrophilic organic solvent, but is generally extracted with ethanol, and further with methanol, butanol, acetone, or a mixture thereof. It is also possible to replace some of these organic solvents with water. Although the amount of water varies greatly depending on the type of hydrophilic organic solvent, in the case of ethanol, extraction with a solvent containing 50% or more of water reduces the whitening component. In addition,
In the case of water alone or ethanol, even if the water content is 50% or more, the whitening component is reduced, and there is no point in taking such an extraction method. You can choose.

This extract is used as a raw material for other cosmetics such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3-butylene glycol, and various chemicals. Add
Various dosage forms of cosmetics can be prepared. For example, a use form may be considered depending on the purpose, such as a lotion, a cream, an emulsion, a pack, and the like.

The use concentration of the extract of the present invention is naturally affected by the purpose of use and other ingredients, and is not particularly limited, but is usually 0.0001 to 5%. is there.

[0009]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, which should not be construed as limiting the present invention. First, production examples of the extract (dry solid) used in Examples and Comparative Examples will be described.

(Production Example 1) 200 ml of methanol was added to 20 g of dried cranes and the mixture was refluxed and cooled for 3 hours. This was filtered and freeze-dried. The yield was 1.98 g.

(Production Example 2) To 10 g of dried cranes, 300 ml of ethanol was added and extracted with a Soxhlet extractor. The extract was freeze-dried. The yield was 1.87 g.

(Example 1) Lotion (parts by weight) Methanol extract of Sengaku moss from Production Example 1 (dry product) 1.0 Ethanol 80.0 Purified water19.0  100.0

(Example 2) Cream (parts by weight) A. Squalane 20.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Ethanol extract of Sengaku grass of Production Example 2 (dry product)1.0  36.0 B.R. Purified water 55.9 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0 Glycerin 5.0 Methyl paraoxybenzoate0.1  64.0 A and B were respectively weighed, heated to 70 ° C., and A was added to B.
Is slowly added with stirring, and then slowly stirred.
Cooled to 0 ° C.

Comparative Example 1 Comparative Example 1 was obtained by replacing the dried extract of Example 1 with water.

Comparative Example 2 Comparative Example 2 was obtained by replacing the dried extract of Example 2 with water and using the B component.

The evaluation was carried out by measuring the tyrosinase activity inhibition rate and the effect of inhibiting active oxygen in Examples 1 and 2 and Comparative Examples 1 and 2 for a 1.0 wt% ethanol solution of the dried extract of Production Example 2. The lotion and cream No. 2 were evaluated by a panel sensory test.

(Tyrosinase activity inhibition rate) Mcllvaln buffer 0.9 ml, 1.66 m
After dissolving 1.0 ml of the M tyrosine solution and the extracted and dried product of Production Example 1 or 2 in ethanol, adding water, and removing the ethanol with an evaporator, 0.1 weight (dry product) / volume 1.0% liquid (sample liquid)
Was placed in a screw vial and heated in a thermostat bath at 37 ° C. for 5 minutes or more. 0.1 ml of tyrosinase solution (manufactured by Sigma, from mushrooms, 914 units / ml) was added,
The temperature was kept in a constant temperature water bath at 37 ° C., and the absorbance was measured at 475 nm after 10 minutes. As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B− (AP)} / B × 100 where A: absorbance of sample specimen B: absorbance of control P: absorbance of sample specimen by coloring (3 times dilution) The results are shown in Table 1. However, the final concentration is
1/3.

[0018]

[Table 1]

(Active Oxygen Suppression Concentration) There is 21% oxygen in the atmosphere, and without it, organisms (except anaerobic ones)
Cannot exist. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. Active oxygen oxidizes fatty acids to form peroxides. Phospholipids in biological membranes of living organisms also oxidize and cause damage. Moreover, it is said that the generated peroxide and active oxygen damage DNA and promote aging. It also affects the mechanism of melanin production from tyrosine and is involved in skin darkening. Suppressing this active oxygen is important for the skin, in other words, one of the important factors required for cosmetics. There are various methods for measuring the effect of suppressing active oxygen, and the following method was used in this case. ph7.8,50mM potassium phosphate buffer (1.3mM DETAPAC containing) 133ml 40 u ni t / ml of catalase of the above potassium phosphate buffer 5 ml 2 mM nitroblue tetrazolium above potassium phosphate buffer 5 ml 1.8 mM xanthine 17 ml 160 ml of the above potassium phosphate buffer 2.4 ml of the mixture of the above reagents, the lyophilized product of the extract of the above Preparation Example 1 or 2 dissolved in ethanol, water was added, and then ethanol was removed with an evaporator. 0.3 ml of a liquid (sample) adjusted to 0.1% by weight (dry matter) / volume%
In addition, xanthine oxidase (sample is water in advance,
When performing the experiment, adjust the above-mentioned potassium phosphate buffer so that the absorbance increases by about 0.02 per minute.)
Add 0.3 ml of the solution and immediately measure the absorbance (560 nm). (Measurement is divided into two to confirm linearity.) (Calculation formula) Inhibition rate = (AB) / A × 100 A: Change in absorbance per minute when water is used as a sample B: Sample Table 2 shows the result of conversion of the absorbance per minute into the 50% inhibitory concentration. However,
The final concentration is 1/9 of the concentration in this table .

[0020]

[Table 2]

(Usage Test) The face of each of 12 women was divided into left and right, one of which was used as an example and the other was used as a comparative example. Examples and Comparative Examples 1 and 2 were used in combination. The following table summarizes the results of the questionnaire with the following criteria. Example is very good 3 Example is considerably better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better -2 Comparative example Table 3 shows the total value of the -3 judgment points as a result.

[0022]

[Table 3]

[0023]

EFFECT OF THE INVENTION The whitening and anti-aging cosmetic composition of the present invention has a whitening effect on the skin, has an active oxygen suppressing effect, prevents aging of the skin, and suppresses fine wrinkles and rough skin by containing the extract of Senkaku grass. It has the effect of preventing and increasing the moist feeling. Since it has been used as a crude drug, its safety is guaranteed.

──────────────────────────────────────────────────続 き Continuation of the front page (72) Inventor Kenji Shimomura 3-16-32 Funae, Ise City, Mie Prefecture (72) Inventor Masami Nakamura 6-32, Ikegami-cho, Toba City, Mie Prefecture (56) References JP-A-57- 209208 (JP, A) (58) Fields investigated (Int. Cl. ⁶ , DB name) A61K 7/48 A61K 7/00

Claims (1)

(57) [Claims] 1. A plant belonging to the genus Rosewood, Echinacea
Water, methanol, butanol, acetone and mixtures thereof
Extract extracted with at least one solvent selected from the compounds
And whitening and anti-aging cosmetics.