JP2698123B2 - Bifidobacterium growth promotion / intestinal harmful bacteria growth inhibitor - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to cotton of evening fruit (hereinafter referred to as cotton) or leaves, petiole and vine of evening meal (hereinafter referred to as leaf stem).
Containing Bifidobacterium as an active ingredient
erium) It relates to a growth promotion / intestinal harmful bacteria growth inhibitor.

Prior art Evening glory is an annual vine plant of the bollaceae, and its fruit is oval with a diameter of about 70 cm. The dried and dried pulp of the fruit is like a string. Cotton is the soft part of the center of the flesh of the evening grape fruit, which is discarded during the production of gourd.

Bifidobacterium is not pathogenic and exists mainly in the intestines of mammals, including humans, and has been shown to have physiological significance such as suppression of intestinal putrefaction products, diarrhea, and improvement of constipation. Is a useful fungus.

In recent years, the immunity-promoting effect of Bifidobacterium bacteria, the preventive effect of bacterial alternation caused by continuous administration of antibiotics, and the inhibitory effect of carcinogenic substances have been clarified. ("Microorganisms", Volume 1, 4
No. 2, p. 1987, Medical Publishing Sensor). in this way,
Bifidobacterium is closely related to the health of mammals including humans, and is attracting attention in the medical field.

As a method of growing Bifidobacterium in the intestine, there are a direct method of orally administering Bifidobacterium and a method of administering a substance used for Bifidobacterium (hereinafter referred to as Bifidobacterium). There is an indirect method of growing fidobacteria. The direct method has problems such as low stability of the Bifidobacterium preparation and a low colonization rate of the bacteria after administration. Recently, the indirect method has been emphasized.

Conventionally, as the bifidogenic factor, N-acetylglucosamine, a derivative thereof, a carrot extract (mainly containing pantethine), rastulose, raffinose, stachyose, maltotriose ("Bifidobacterium", 77
1979, Yakult Honsha Co., Ltd., fructooligosaccharides ("Chemistry and Biology", 21, 291 pages, 1983, Gakkai Shuppan Center), galactooligosaccharides (JP-B-58-20266, JP-B-61-46479). JP, JP-A-60-41449), isomaltooligosaccharides (Presentation of Japanese Society of Nutrition and Food, 19
1986), cyclodextrin (JP-A-57-138385), konjac mannan (“RIKEN intestinal flora symposium III, intestinal flora and nutrition”, p. 89, 1983)
Soy Milk (JP-B-45-9822, JP-A-51-142566, JP-A-55-85390), soymilk extract (JP-A-59-17906), Various substances such as an extract of a non-pathogenic Escherichia coli culture (Japanese Patent Publication No. 50-13359) are known.

Conventionally, it has not been known that the cotton or leaf stem of the evening face promotes the growth of Bifidobacterium and suppresses the growth of intestinal harmful bacteria.

PROBLEM TO BE SOLVED BY THE INVENTION Since the above-mentioned known bifidobacterium is utilized also for other intestinal bacteria in vivo, the growth effect of bifidobacterium is not sufficient, and the production method thereof is complicated. There are drawbacks such as certain and expensive. Therefore, a bifidobacterium having sufficient bifidobacterium growth promoting effect and intestinal harmful bacterial growth inhibitory effect, as well as being inexpensive and easily producible, is extremely useful in industry.

Means for Solving the Problems Already, some of the present inventors have conducted intensive studies on the bifidobacterium, and as a result, the evening meal obtained by processing the evening flesh selectively and remarkably promoted the growth of Bifidobacterium bacteria. (Japanese Patent Application No. 62-123761). The present inventors have found that the cotton or leaf stem of the evening face has the same effect of promoting the growth of bifidobacteria as the pulp, and also has the effect of suppressing the growth of harmful bacteria in the intestine, and completed the present invention.

The bifidobacterium growth promotion / intestinal harmful bacteria growth inhibitor of the present invention is a powder or granule of cotton or leaf stem of evening face,
It is processed into a desired shape, such as a tablet, and may or may not be sterilized.

The sterilized growth-promoting / intestinal harmful bacteria growth inhibitor of the present invention has a steam pressure of 0.2 to 0.4 kg / cm ² , and a sterilization time of 5 to 20 minutes by steam sterilization or an alcohol addition amount of 15 to 150 g / kg (evening face dried ), And can be manufactured by alcohol sterilization for 1 to 30 days of sterilization. The sterilized growth promoting / intestinal harmful bacteria growth inhibitor has a general viable cell count of 5 × 10 ³ cells / g or less, is negative for Escherichia coli and Salmonella, and has a water content of 8% or less,
Very safe for food hygiene.

Additives may be added to the growth-promoting / intestinal harmful bacteria growth inhibitor of the present invention. Examples of the additives include sweet corn, carrot powder, corn fiber, apple fiber, pumpkin powder, and fiber components such as alginic acid. , Lactose, excipients such as starch, sweeteners such as sucrose, maltose, sorbitol, mannitol, nutritional supplements such as milk powder, meat extract, vitamins, gum arabic powder, polyvinylpyrrolidone, hydroxypropylcellulose, sodium carboxymethylcellulose, etc. And a lubricant such as magnesium stearate, calcium stearate, lubri wax, talc, etc., and may be appropriately selected and used depending on the shape and / or taste of the foodstuff for promoting growth.

Of the growth promoting / intestinal harmful bacteria growth inhibitor of the present invention, powdery and granular forms may be added to soup ingredients, sprinkles or to confectionery, noodles, tofu, and other foods. In addition, as a feed additive for livestock such as cattle and pigs,
It can also be used as a feed additive for pets such as dogs and cats.

Action The action of the bifidobacterium growth promotion / intestinal harmful bacteria growth inhibitor of the present invention will be described.

As samples, evening powder cotton powder produced by the same method as in Production Example 1, evening powder leaf stem powder produced by the same method as in Production Example 6, sterilized evening powder cotton powder produced by the same method as in Production Example 5, and Production Example 10 A pasteurized evening stalk powder produced in the same manner as in Example 1 was used. A known bifid factor was used as a control.

Test 1 A Negishi agar medium (Bacteriological Journal of Japan, Vol. 13, p. 519, 1958) was used as a basal medium for searching for a bifidobacterial factor, and 0.5% of the sample was added to the medium. Bifidobacterium longum (ATCC 15707) was applied as described above. This was cultured at 37 ° C. for 48 hours, and the state of proliferation was observed.
Table 1 shows the results.

In the table, + in the column of the growth state indicates that the larger the number, the better the growth state.

As is clear from Table 1, the evening face cotton powder, the pasteurized evening face cotton powder, the evening face leaf stem powder and the pasteurized evening face leaf stem powder have the same effect as the evening face fruit powder in promoting the growth of bifidobacteria which is superior to the conventional bifidobacterium factor. Was.

Test 2 Evening cotton powder and evening leaf stem powder were converted from a base medium, Tomarelli modified medium (Journal of Biochemical Chemistry, 181: 879, 1949)
Was added, and Bifidobacterium longum (ATCC 15707) was cultured at 37 ° C. for 48 hours. After the culture, a culture solution of Bifidobacterium longum was applied to a BL agar medium, and cultured at 37 ° C. for 48 hours, and the number of cells in the medium was measured. Table 2 shows the results.

As is clear from Table 2, the evening cotton cotton powder and the evening leaf stem powder had a concentration of 0.10 to 2.
It shows a 1000-fold growth promoting effect at 00%.

Test 3 Evening cotton powder, evening leaf stem powder and evening meal flesh powder were used as samples.

Each sample was added to the Tomarelli modified medium, and Bifidobacterium and other enterobacteria were cultured at 37 ° C for 48 hours. After culturing, apply the culture solution to BL agar medium and
After culturing for 48 hours, the number of bacteria was measured. Table 3 shows the results.

As is clear from Table 3, evening face cotton powder and evening face leaf stem powder show a remarkable growth-promoting effect on bifidobacteria similarly to evening face pulp powder, while enterococci, Pseudomonas aeruginosa,
It is not used for intestinal harmful bacteria such as Escherichia coli and C. perfringens, but rather has a growth inhibitory effect on these bacteria.

Production Example 1 Evening cotton was dried for one day in the sun and then dried at 70 ° C. for 5 hours to obtain evening cotton having a water content of about 3%. 30 kg of this dried product
Was ground with a pulverizer (manufactured by Hosokawa Iron Works) to obtain 24 kg of evening cotton powder.

Production Example 2 After mixing 950 g of the powder obtained in Production Example 1 and 40 g of hydroxypropyl cellulose in a mixing stirrer for 10 minutes, purified water was added and kneaded, followed by hot-air drying. After allowing this to cool, it is sized with an oscillator (manufactured by Kikusui Seisakusho) and the evening cotton cotton granules 96
0 g was obtained.

Production Example 3 After mixing 495 g of the granules obtained in Production Example 2 and 5 g of magnesium stearate in a mixing stirrer for 10 minutes, the mixture was tableted to obtain 4400 evening tablet cotton tablets of 110 mg each.

Production Example 4 After 30 days of 30 kg of sun-dried cotton on the sun dried for 20 minutes under a vapor pressure of 0.2 kg / cm ² , the water content was reduced to 60% until the water content became about 3%.
It dried with hot air at ° C. Then, this was crushed to obtain 25 kg of sterilized evening cotton powder.

This powder had a general viable cell count of 3.4 × 10 ² cells / g and was negative for Escherichia coli and Salmonella.

Production Example 5 2.7 kg of ethanol was sprayed onto 30 kg of sun-dried cotton, which had been sun-dried for 1 day, sealed, allowed to stand at room temperature for 5 days, and then pulverized to obtain 26 kg of sterilized cotton powder.

This powder had a general viable cell count of 2.5 × 10 ² / g, and was negative for Escherichia coli and Salmonella.

Production Example 6 Evening leaf stems were dried for one day in the sun and then dried at 70 ° C. for 5 hours to obtain evening leaf stems having a water content of about 3%. 30 kg of this dried product
Was pulverized with a pulverizer (manufactured by Hosokawa Iron Works) to obtain 23 kg of evening face leaf stem powder.

Production Example 7 After mixing 950 g of the powder obtained in Production Example 6 and 40 g of hydroxypropyl cellulose in a mixing stirrer for 10 minutes, purified water was added and kneaded, followed by hot-air drying. After allowing it to cool, it is sized with an oscillator (manufactured by Kikusui Seisakusho) and the evening leaf stem granules 96
0 g was obtained.

Production Example 8 After mixing 495 g of the granules obtained in Production Example 3 and 5 g of magnesium stearate in a mixing stirrer for 10 minutes, the mixture was tableted to obtain 4400 tablets of 110 mg of evening globe leaf stem tablets.

Production Example 9 30 kg of evening sun leaf stems dried in the sun for one day were sterilized under a vapor pressure of 0.2 kg / cm ² for 20 minutes, and then 60 kg until the water content became about 3%.
It dried with hot air at ° C. Then, this was crushed to obtain 23 kg of sterilized evening stalk powder.

This powder had a general viable cell count of 6.3 × 10 ² cells / g, and was negative for Escherichia coli and Salmonella.

Production Example 10 2.7 kg of ethanol was sprayed onto 30 kg of the sun-dried evening globe leaf stem, sealed, allowed to stand at room temperature for 5 days, and then pulverized to obtain a sterilized evening grape leaf stem powder of 25 kg.

This powder had a general viable cell count of 3.1 × 10 ² / g, and was negative for Escherichia coli and Salmonella.

EXAMPLE For 5 healthy adults, evening ginger cotton powder produced by the same method as in Production Example 1 was 2 g / day for 7 days, and evening glow leaf stem powder produced by the same method as in Production Example 6 was given 2 g per day. 7 days,
Each was administered before meals. On day 7 of each administration, feces of each individual were collected, cultured on a BS medium, and the number of bifidobacteria, lactic acid bacteria, and Escherichia coli grown were measured, and the average value was obtained. Table 4 shows the results.

As is evident from Table 4, it was confirmed that the evening meal cotton powder and the evening leaf stem powder selectively and significantly promoted the growth of bifidobacteria in the intestine.

Effect of the Invention The agent for promoting the growth of bifidobacteria and the growth of harmful intestinal bacteria according to the present invention selectively and remarkably promote the growth of bifidobacteria, and suppress the growth of harmful bacteria in the intestine. In addition to helping maintain health, it is also expected as a drug such as an immunostimulant or an anticancer drug. Further, the present invention contributes to the development of agriculture because it enables effective use of evening face cotton and leaf stems, which have not been used until now.

Claims (2)

(57) [Claims] 1. An agent for promoting the growth of bifidobacteria, which comprises the cotton of evening fruit as an active ingredient and suppresses the growth of harmful intestinal bacteria. 2. An agent for promoting the growth of bifidobacteria, which comprises evening leaves, petiole and vine as active ingredients and suppresses the growth of intestinal harmful bacteria.