KR0144566B1 - Bifidobacteria growth promoter - Google Patents

Abstract

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Description

Bifidus Growth Promoters

The present invention relates to a bifidus bacterial growth promoting agent. In detail, the present invention relates to the promotion of bifidus bacteria growth containing pullulan and / or dextran as an active ingredient.

Bifidobacterium is a bacterium belonging to the Gram-positive, anaerobic bacilli knitting (偏性嫌氣性竿菌) as Bifidobacterium (Bifidobacterium 屬) of polymorphism. See Bergy's Manal of Systemic Bacteriology, first edition, pp. 1418-1434, Williams Williams, United States (1984)]. Bifidobacteria are not pathogenic, and while normally inhabit the large intestine of humans and animals form the intestinal microflora (腸內菌叢) together with other bacteria. While Captain Rich in animal intestinal microflora (腸內菌叢) together with other bacteria account for most of the bifidobacteria. After weaning foods become complicated, the rate of bifidus bacteria gradually decreases to about 10% in adults and sometimes not detected at all.

Bifidobacteria has been found to play an important role in promoting beauty and health maintenance by

(1) In the intestine, organic acids such as acetic acid and lactic acid are produced to lower the pH and inhibit the growth of various infectious bacteria.

(2) Suppresses the growth of bufe bacteria and inhibits the production of harmful substances such as ammonia, indole, cresol and paracresol, which are likely to occur during metabolism of amino acids and proteinaceous substances.

(3) Properly stimulate the intestines peristalsis (peristalsis) moderately ( 정장 作用 ) to work.

(4) We biosynthesize vitamins.

(5) treatment and prevention of hyperamonia, hepatic encephalopathy, etc.

These various functions led to the examination of methods for increasing the share of bifidus bacteria in the intestinal flora. For example, a method of ingesting bifidus itself has been attempted. However, in this method, even if the ratio of bifidus bacteria is increased, it is only a temporary phenomenon, and it is difficult to stably reproduce bifidus bacteria and it is difficult to maintain a high proportion of occupied bacteria in the enterobacterial layer.

Recently, a method of orally ingesting various sugars as an intestinal bifidus growth promoter has been proposed. For example, lactulose, raffinose, lactosucrose (or 0-β-D-galactosyl- (1 → 4) 0-α-D-glucosyl- (1 ← 2) -β A method of ingesting oligosaccharides such as -D-fructoside), fructooligosaccharide, galacto oligosaccharide and xylo oligosaccharide is also proposed. However, oligosaccharides not only have an unnecessary sweet taste, but also have a large individual difference in their intake and a narrow dosage range. And too little intake will not work, too much can cause diarrhea. In addition, these oligosaccharides are generally absorbent, unstable with heat, and poor in processability.

Meanwhile, there are proposals to use a high-molecular polysaccharide of tasteless (無味). For example, the use of hemicellulose, in particular, the use of a complex or complex polysaccharide (heteroglycan) in which arabinose and xylose derived from grains are combined is proposed. (Reference: Japanese Patent Laid-Open 63-165325). However, it is difficult to carry out this proposal because the complex polysaccharide has a complicated preparation method and a low yield.

And reports on the use of simple polysaccharides (homoglycan) in detail (Chemical Pharmaceutical Bulletin Vol. 26, No. 11, pp-3306-3311 (1978)). According to the results of comparative experiments on the effect of promoting the growth of bifidus bacteria using various simple polysaccharides, inulin, a type of homofuruktan, has a remarkable growth promoting effect, but homoglucan is a pullulan, In the text, none of the soluble starch or the like has any effect.

Although it is clear that homoglucan does not show the effect of promoting the growth of bifidus as described above, the present inventors focused on homoglucans which are harmless, harmless and stable, and searched for the effect of promoting the growth of bifidus in homoglucan. Therefore, the main purpose of the present invention is to establish a new bifidus growth promoter.

The present inventors have reviewed the above report in detail, and any experiments in which homoglucans such as pullulan, dextran, soluble starch, and the like have no effect of promoting bifidus proliferation are by in vitro experiments, and the upper body ( invio), it is not clear what is. Thus, the present inventors conducted a field test using direct in vivo using highly stable pullulan, dextran, and soluble starch. Surprisingly, pullulan, soluble starch, and dextran are effective in promoting the growth of bifidus bacteria. In particular, it was confirmed that the effect of pullulan is remarkable, and developed a bifidus growth promoting agent containing the pullulan and / or dextran of the present invention as an active ingredient. In the field test, the bifidus growth promoter (i) preferentially propagates the bifidus bacteria in the intestine to produce organic acids, lower the pH, exhibit moderate effects of peristalsis, intestinal action, and (ii) conventional, dietary An increase in the amount of fecal matter, which was considered to be the action of the fiber (dietarg fiber), was confirmed, and it was found to have a characteristic of exhibiting a stronger dressing effect.

Pullulan and dextran used in the present invention, unlike oligosaccharides in the effect of promoting the growth of bifidus bacteria, individual differences are small, even if the intake is reduced relatively satisfactory effect, even if relatively large, there is no fear of causing diarrhea ideal bifidus It was confirmed that the bacterium promotes bacterial growth.

The bifidus bacteria growth promoter of the present invention contains pullulan and / or dextran and can promote the growth of bifidus bacteria in the intestine by oral ingestion or intubation feeding. The method for producing such accelerators contains pullulan or dextran itself and, if necessary, contains other materials suitable for pullulan or dextran to process solids, semi-solid or liquid bifidus growth promoters.

Other substances include, for example, nutrients such as amino acids, proteins, unsaturated fatty acids, lipids, sugars, vitamins and minerals; Edible extracts such as chlorella extract, yeast extract, mushroom extract, medicinal ginseng extract, aloe extract, grow up extract, seaweed extract, oyster extract, sauerkraut extract, and beef extract; Fluids such as fruit juice, vegetable juice, soybean milk milk, milk serum, yogurt and the like; Pastes such as sesame paste, chestnut paste, bread, dough, luxury starch, paste, gelatinized starch paste and pastry sweet pot; Useful microorganisms such as Bifidobacteria, Lactobacillus, and Yeast; Bifidus growth-promoting oligosaccharides such as lactulose, raffinose, lactosucrose, isomaltooligosaccharide, fructooligosaccharide, galactooligosaccharide and xyl oligosaccharide; Dietary fibers such as cereal bran, pectin, locast bean gum, polydextrose ^R , and the like; In addition, a gastrointestinal drug, a suiting agent, a stool excretion accelerator, etc. may be appropriately blended as necessary to increase the effect of the bifidus bacterium growth promoting agent of the present invention, and may further impart another effect.

In particular, one or more oligosaccharides of the bifidus growth promoting oligosaccharide may be advantageously mixed with pullulan and / or dextran in a range of about 0.1 to 10 times on a solid basis.

Further, by processing by, in addition to the various materials described above in the manufacture of bifidobacteria growth promoting agent of the present invention contains a properly seasoned claim (調味制), colorants flavors, stabilizers, extenders, such as intake of the consumer for the bifidobacteria growth promoting agent The palatability can be satisfied.

Both pullulan and dextran used in the present invention are known water-soluble and low-viscosity homoglucans, and their molecular weight is 2,000 to 5,000,000, usually about 10,000 to 2,000,000. In particular, pullulan is characterized by high salt resistance and excellent stability in salt solution, high adhesion and adhesion, and excellent film forming ability, moldability and water solubility.

In the present invention, the method of containing pullulan or dextran may include mixing, kneading, dissolving, dipping, spreading, coating, coating, powdering, injecting, solidifying, molding, and the like of pullulan and / or textran itself or a solution thereof. One or more known methods may be used as appropriate.

The method of using the bifidus bacteria growth promoter thus obtained can be appropriately selected depending on the type of bifidus bacteria growth promoter, the ingestion method, the intake amount, the increase in the amount of pullulan or dextran contained therein, the intake frequency and the like. In general, adults should consume about 0.1 to 100 g, preferably about 0.5 to 20 g, per day as the amount of pullulan and / or dextran per day.

Ingestion of the bifidus bacteria growth promoter of the present invention preferentially propagates bifidus bacteria in the intestine, particularly in the large intestine, thereby producing organic acids such as acetic acid and lactate and lowering the pH. In addition, it inhibits the growth of infectious bacteria and rot bacteria, and suppresses the generation of harmful substances that are likely to occur in metabolic processes such as amino acids and proteins. On the other hand, moderate stimulation of the intestines to properly promote peristalsis, as well as increase the amount of feces and significantly increase the effect of the suit to prevent constipation. Therefore, the bifidus growth promoting agent of the present invention can be advantageously used for the treatment and prevention of beauty, health maintenance, prevention of adult diseases, promotion of recovery after illness, hyperammonemia, hepatic brain disease and the like regardless of gender.

In addition, the bifidus bacterium growth promoting agent of the present invention exhibits its effects in livestock such as pigs, dogs and cats, poultry such as canaries, parakeets and chickens, and other breeding animals such as bees, silkworms and fish. As a result, the bifidus growth promoting agent of the present invention can be advantageously used for the purpose of preventing infection, preventing diarrhea, promoting non-bathing, promoting spawning of breeding animals, and suppressing odor of feces.

Next, the test will be described in detail.

Experiment

Effect of Homulglucan on the Effect of the Growth of Bifidus Bacteria

Eight subjects (mean age 33.4 years old, average weight 62.8 kg male) were fed 10 g of pullulan, dextran, or soluble starch, dissolved in hot soup once daily for lunch and continued for 14 days.

The experiment sequence was followed by a 14-day comparative test period after the pullulan intake experiment, followed by a dextran intake experiment, and then a soluble starch intake experiment. Fecal weight (g / day), fecal weight state change, fecal pH, total bacteria per gram of feces, percentage of bifidus in total bacteria, and relative changes in total bifidus before and after ingestion for 14 days The average value of 8 people was calculated.

Among them, the total bacterial count was measured according to a known method (see Tomotari Mitsuoka, A Color Atlas of Anaerobic Bacteria, pp-53-65, Sobunsha, Tokyo Japan (1984)). That is, the number of bacteria was measured with the determination of which bacteria group (genus) the cloned expression belongs to using 13 kinds of media except MIO medium. The number of bacteria in each cell group was the number of cells in the medium showing the highest number of fungi as the real cell count. The total number of bacteria in each of the bacterial groups thus obtained was defined as the total number of bacteria in the feces. The share (%) of bifidus bacteria to total bacteria was calculated by multiplying the number of bacteria of bifidus by the total number of bacteria.

The relative change in the total number of Bifidobacteria was calculated by multiplying the number of Bifidobacteria by the fecal weight per 1g of feces, and the number of Bifidobacteria after intake for 14 days with the number of Bifidobacteria before ingestion as 100 as a relative value. The experimental results are shown in each table. The first table shows the change in the fecal flora of the subject, and the second table shows the change in fecal weight, fecal pH, and total bifidus bacteria in the feces.

Note: The numerical value represents the logarithmic mean ± standard deviation of the number of bacteria in 1 g of feces.

Note: The number of bacteria indicates the logarithm (Log10) mean ± standard deviation of the number of bacteria in 1 g of feces, and the other values indicate the mean or average ± standard deviation.

As is clear from the results of Tables 1 and 2, ingestion of pullulan and dextran increases fecal weight excreted per day, unlike soluble starch, and the number of bifidus bacteria per 1g of feces It can be seen that the ratio of the number of bifidus bacteria to the total number of bacteria increases by about 1.5 to 2 times, about 2 to 4 times that of the total number of bacteria, and about 0.5 to 1.0 in fecal pH. It turned out to be. In particular, these effects were found to be remarkably superior to pullulan in any case. Accordingly, it has been found that pullulan and / or dextran not only function as a bifidus growth promoter but also have a function as a dietary fiber. Therefore, it can be advantageously used for the prevention and treatment of adult diseases such as beauty, health maintenance, hypertension, diabetes, myocardial infarction, malignant tumors, promotion of recovery, illness, hepatic encephalopathy.

Next, some examples of the present invention will be described with respect to the bifidus growth promoter for ingestion or cervical ingestion.

Example 1

Bifidus growth promoter for oral intake

) 5중량부 및 적당량의 물을 균일히 혼합하여 과립 성형기에서 과립 모양의 비피더스균 증식 촉진제를 제조하였다.100 parts by weight of corn bran fine powder, 100 parts by weight of pullulan having a molecular weight of about 3,000,000, oligosaccharide syrup (registered trademark of Hayashibara Co., Ltd., Japan)

5 parts by weight and an appropriate amount of water were uniformly mixed to prepare a granular bifidus growth promoter in a granulation machine.

The agent can be taken orally as it is, and may be made into tablets in a tablet press again as needed to facilitate oral ingestion.

In some cases, it can be dissolved or consumed in rice porridge, milk, oatmeal, soup, juice, etc., or it can be mixed with other raw materials in the cooking process to process various oral intakes such as health foods, medicines, and feeds. It can be advantageously used.

In addition to the effect of promoting the growth of bifidus bacteria and the dietary fiber effect, the present invention can be advantageously used to improve the maintenance of beauty health, prevention of adult diseases, improvement of illness, post-mortem recovery, hyperamnemia, hepatic encephalopathy.

In addition, it can be advantageously used for the prevention of infection of domestic animals such as livestock and poultry, prevention of diarrhea, promotion of fattening, and control of feces.

Example 2

Bifidus growth promoter for oral intake

) 100중량부, 분말당 40중량부, 쇼트닝 20중량부, 당에스테르 1.5중량부, 커피 분말 6중량부, 분유 3중량부, 젤라틴을 3배의 열수로 팽유시킨 것 4중량부, 이소말토올리고당 시럽 70중량부 및 물 15중량부를 미리 혼합한 후 니이더(kneader)로 반죽하고, 성형, 포장하여 커피맛을 가진 과자 형태의 비피더스균 증식 촉진제를 제조하였다.15 parts by weight of pullulan of molecular weight approximately 200,000, powder maltose (product of Hayashibara Japan, registered trademark SUNMALT

) 100 parts by weight, 40 parts by weight of powder, 20 parts by weight of shortening, 1.5 parts by weight of sugar ester, 6 parts by weight of coffee powder, 3 parts by weight of powdered milk, 4 parts by weight of gelatin in 3 times hot water, isomaltoligosaccharide 70 parts by weight of syrup and 15 parts by weight of water were mixed in advance, kneaded with a kneader, molded and packaged to prepare a bifidus bacterium growth promoter in the form of a confectionary having a coffee taste.

The present invention is a bifidus growth promoting agent for oral ingestion that can satisfy consumer's palatability, can exert not only the bifidus growth promoting effect but also the effect of dietary fiber and can be advantageously used for promoting beauty and health maintenance.

Example 3

Bifidus growth promoter for oral intake

) 220중량부, 덱스트란(분자량 약 500,000) 60중량부, 식용색소 적당량을 바닥이 평평한 솟에 넣고 물 210 중량부를 가하여 혼합하면서 가열하여 117℃까지 농축한후, 농축물에 꺼내어 여기에 수크로오스 분말 30중량부, 멘톨 0.1 중량부를 혼합하고 냉각성형, 포장하여 박하맛을 내는 추잉검 모양의 비피더스균 증식 촉진제를 제조하였다.Sucrose 220 parts by weight, scrambled starch syrup (product of Hayashibara Japan, registered trademark COUPLING SUGA

) 220 parts by weight, dextran (molecular weight: about 500,000), 60 parts by weight of food coloring are added to a flat bottom and 210 parts by weight of water are added to the mixture, heated and concentrated to 117 ° C. 30 parts by weight, 0.1 parts by weight of menthol were mixed, cooled, and packed to prepare a chewing gum-shaped bifidus bacterium growth accelerator that had a taste of mint.

As in Example 2, the present invention is a bifidus growth promoting agent for oral ingestion that can satisfy consumer's palatability, and exhibits not only the bifidus growth promoting effect but also the effect of dietary fiber and can be used advantageously for promoting the maintenance of beauty health.

Example 4

Bifidus growth promoter for oral intake

1,000 parts by weight of water were heated to 70 ° C, and 100 parts by weight of rapid-set pectin (HMP) and 1,000 parts by weight of sucrose-bonded starch syrup were added. When the mixture starts to boil, add 8,900 parts by weight of sucrose-bonded starch syrup and 100 parts by weight of pullulan (molecular weight: about 10,000), heat it to 104 ° C, cool it to 95 ° C, and add an appropriate amount of lemon flavor and food coloring. Citric acid solution warmed to ℃ was added to pH 3.3, and then mixed, dispensed, cooled, and packaged to prepare a bifidus bacteria growth promoter in the form of lemon jelly.

The present agent exerts not only bifidus growth promoting effect but also dietary fiber effect, which can be advantageously used for the maintenance of beauty health.

Example 5

Bifidus growth promoter for oral intake

After sterilizing 10,000 parts by weight of skim milk at 80 ° C. for 20 minutes, it was cooled to 40 ° C., and 300 parts by weight of lactic acid bacteria as a starter were added thereto, followed by fermentation at 35 to 37 ° C. for 10 hours. Subsequently, after homogenization, 4,000 parts by weight of isomerized syrup, 2,000 parts by weight of sucrose, and 170 parts by weight of pullulan (molecular weight 20,000) were mixed and dissolved and maintained at 70 ° C. for sterilization.

After cooling, a small amount of fragrance was added and bottle packed to prepare a bifidus bacterium growth accelerator in the form of lactic beverage.

The present agent exerts not only bifidus bacteria growth promoting effect, but also a dietary fiber effect, and can reduce the intestinal pH to inhibit the growth of decayed bacteria to prevent constipation.

Example 6

Bifidus growth promoter for oral intake

10 parts by weight of pullulan (molecular weight: 100,000) was dissolved in 1,000 parts by weight of vegetable juice mainly made of tomato juice, and sterilized by heat sterilization according to a conventional method, thereby preparing a growth agent for bifidus bacteria in the form of juice.

It is an ideal health drink because it not only disperses vitamins and minerals possessed by vegetable juice, but also promotes bifidus bacteria growth and dietary fiber effect.

Example 7

Bifidus growth promoter for oral intake

25 parts by weight of pullulan (molecular weight: about 80,000), 25 parts by weight of lactosucrose, 1 part by weight of tricalcium phosphate, 1 part by weight of sucrose ester, and powdered fragrance, and the tableting machine is about 350 mg according to a conventional method. It was compressed into to prepare a bifidus growth promoter in the form of a tablet.

The agent is to take about 1 to 40 tablets per day, preferably about 2 to 20 tablets per day to exert the effect of promoting the growth of bifidus bacteria and dietary fiber effect.

Example 8

Bifidus growth promoter for oral intake

) 18중량부, 0.1 w/w% 비피더스균 함유 말토오스 분말 2중량부, 제3인산 칼슘 1중량부, 수크로오스 에스테르 1중량부 및 분말 향료 적당량을 균일하게 혼합한 후 통상의 방법에 따라 1정의 무게가 약 400mg되도록 타정기로 타정하여 정제형태의 비피더스균 증식 촉진제를 제조하였다.30 parts by weight of pullulan (molecular weight approximately 100,000), anhydrous crystalline malty top (product made by Shoji Hayashibara Japan, registered trademark MABIT)

) 18 parts by weight, 2 parts by weight of maltose powder containing 0.1 w / w% bifidus bacterium, 1 part by weight of tricalcium phosphate, 1 part by weight of sucrose ester, and an appropriate amount of powdered perfume, and then weight of 1 tablet according to a conventional method. Was compressed into a tableting machine so as to about 400mg to prepare a bifidus bacteria growth promoter in tablet form.

This agent is to take about 1 to 40 tablets, preferably about 2 to 20 tablets a day for adults to exert the effect of promoting the growth of B. f. Bacteria and dietary fiber.

Example 9

Bifidus growth promoter for oral intake

20 parts by weight of textran (molecular weight: about 90,000), 30 parts by weight of lactosucrose, 1 part by weight of tricalcium phosphate, 1 part by weight of sucrose ester, an appropriate amount of powdered food coloring, and a suitable amount of powder fragrance, according to a conventional method The tablets were tableted with a tableting machine so that the weight of one tablet was about 680 mg to prepare a bifidus bacterium growth promoter.

The agent is to take about 1 to 40 tablets, preferably about 2! 20 tablets per day for adults to exert the effect of promoting bifidus growth and dietary fiber.

Example 10

Bifidus bacteria growth accelerator for cervical intake

) 580중량부, 건조난황 190중량부, 탈지분유 209중량부, 풀룰란(분자량 약 50,000) 15중량부, 염화 나트륨 4.4중량부, 염화 칼슘 1.85중량부, 황산 마그네슘 4중량부, 티아민 0.01중량부, 아스코르브산 나트륨 0.1중량부, 비타민 E 아세테이트 0.6중량부 및 니코틴산아미드 0.04중량부로 이루어진 조성물을 조제하고, 이중 25g씩을 라미네이트 알루미늄제 작은 포대에 충전하고, 히이트 씰(heat seal)하여 사용할 때 용해 형태의 비피더스균 증식 촉진제를 제조하였다.Crystalline α-maltose (product of Hayashibara Japan, registered trademark FINETOSE

) 580 parts by weight, dried egg yolk 190 parts by weight, skim milk powder 209 parts by weight, 15 parts by weight of pullulan (molecular weight of about 50,000), sodium chloride 4.4 parts by weight, calcium chloride 1.85 parts by weight, magnesium sulfate 4 parts by weight, thiamine 0.01 parts by weight , 0.1 parts by weight of sodium ascorbate, 0.6 parts by weight of vitamin E acetate and 0.04 parts by weight of nicotinic acid amide. Bifidus bacteria growth promoter of was prepared.

The present agent does not require low temperature storage and is stable for a long time at room temperature, and has excellent solubility and dispersibility.

This drug is dissolved in 1 bag of about 150 ~ 300ml of hot water, and taken by nasal cavity, esophagus and stomach according to the landscape method, and it promotes the growth of bifidus bacteria and the effect of dietary fiber, and promotes the recovery of patients.

In particular, it promotes the growth of bifidus bacteria in the large intestine, and reduces the pH to suppress the production of harmful substances by decaying substances.

In addition, it is possible to increase constipation and predict constipation that is likely to occur in patients.

On the other hand, the present agent can be advantageously used as a bifidus growth promoting agent for oral or cerebral intake for not only humans but also livestock.

Example 11

Bifidus bacteria growth accelerator for cervical intake

16.5 parts by weight of crystalline α-maltose, 4.05 parts by weight of sucrose, 3.2 parts by weight of mandarin orange juice powder, 1.0 part by weight of dextran (molecular weight of about 40,000), 0.11 part by weight of citric acid, 0.02 part by weight of ascorbic acid and 0.1 part by weight of orange powder A negative composition was prepared, and 400 g of each was filled and sealed in a screw incision can to prepare a bifidus growth promoter in a dissolution form.

This agent is good in stability and solubility like Example 10.

The present agent is dissolved in about 100 g of hot water of about 100-150 ml and ingested to the patient by the cervical method similarly to Example 10. This intake promotes the recovery of patients by providing nutritional supplementation, bifidus growth and dietary fiber effects.

As described above, the present inventors newly discovered that pullulan and dextran have an effect of promoting bifidus growth in vivo. Provided are bifidus bacteria growth promoters containing pullulan and / or dextran as active ingredients of the present invention.

This bifidus growth promoting agent is orally ingested or taken in the cervix to preferentially propagate bifidus in the intestine, produce organic acids such as acetic acid and lactic acid, and lower the pH. Such oral or cervical ingestion inhibits the growth of infectious bacteria and rot bacteria, and suppresses the generation of harmful substances that are likely to occur in metabolic processes such as amino acids and proteins. In addition, the Bifidobacteria growth enhancer moderately stimulates the intestines to moderate peristlsis as well as increases the amount of feces, significantly improving the effect of dressing and preventing constipation.

Therefore, the growth promoting agent of the bifidus bacterium of the present invention is a cosmetic, maintenance and promotion of health, prevention of adult diseases such as hypertension, diabetes, myocardial infarction, malignant tumor, recovery of sickness and disease, hyperammonemia, hepatic brain disease, etc. It can be advantageously used for prevention.

In addition, the bifidus bacterium growth promoting agent of the present invention can be advantageously used for the purpose of promoting bifidus bacterium growth and preventing infection, diarrhea prevention, fattening, spawning, and fecal malodor control even in breeding animals such as leather and poultry.

Because pullulan and dextran are harmless, non-toxic, high in stability and high in industrial productivity, the bifidus growth promoter of the present invention has great significance in medicine, health food and feed industry.

One preferred embodiment of the present invention has been described by way of examples, but the present invention is not limited thereto. Various applications and modifications are possible from them, all of which are based on the gist of the present invention and therefore fall within the scope of the present invention.

Claims (14)

An enteric bifidus bacterium growth promoter of an animal, including humans, containing as an active ingredient pullulan, dextran, and one kind selected from the group consisting of these. The bifidus growth promoter according to claim 1, wherein the molecular weight of pullulan is 2,000 to 5,000,000. The bifidus growth promoter according to claim 1, wherein the dextran has a molecular weight of 2,000 to 5,000,000. 2. The bifidus growth promoter according to claim 1, wherein the dose is about 0.1-100 g per day, per adult, based on the amount of pullulan and / or dextran. The method of claim 1, wherein the promoter is a bifidus bacteria growth further comprising one selected from the group consisting of nutrients, vitamins, minerals, edible extracts, liquid foods, edible pastes, microorganisms, oligosaccharides, dietary fibers, medicines and mixtures thereof. accelerant. 6. The bifidus growth promoter according to claim 5, wherein the weight ratio of oligosaccharide to pullulan and / or dextran is about 0.1 to 10 based on dry solids. The method according to claim 5, wherein the oligosaccharide is lactose, raffinose, lactosucrose (or 0-β-D-galactosyl- (1 → 4) -0-α-D-glucosyl- (1 ← 2) -β-D-furoctoside). A bifidus growth promoting agent which is one kind selected from the group consisting of isomaltooligosaccharide, fructooligosaccharide, galactooligosaccharide and xyl oligosaccharide. The bifidus growth promoter according to claim 1, which is solid, semisolid or liquid. The bifidus growth promoter according to claim 1, which is used for oral administration or cervical administration. The Bifidobacterium growth promoting agent according to claim 1, wherein the Bifidobacterium bacterium is a Bifidobacterium strain. The bifidus growth promoting agent according to claim 1, which is used for maintaining and / or promoting health and beauty. The bifidus growth promoting agent according to claim 1, which is used for preventing adult diseases. The bifidus growth promoting agent according to claim 1, which is used to promote health recovery during or after illness. The bifidus growth promoter according to claim 1, which is used for the treatment and / or prevention of hyperammonia and hepatic encephalopathy.