JP2698128B2 - Bifidobacterium growth promotion and intestinal harmful bacteria growth inhibitor - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to the promotion of the growth of Bifidobacterium containing syrup-like substances (hereinafter, referred to as evening syrup) extracted from evening glory fruit as an active ingredient, and the intestinal growth. The present invention relates to a growth inhibitor for harmful bacteria.

Prior art Evening glory is an annual vine plant of the bollaceae, and its fruit is oval with a diameter of about 70 cm. The dried and dried pulp of the fruit is like a string.

Bifidobacterium is not pathogenic and exists mainly in the intestines of mammals, including humans, and has been shown to have physiological significance such as suppression of intestinal putrefaction products, diarrhea, and improvement of constipation. Is a useful fungus.

In recent years, the immunity-promoting effect of Bifidobacterium bacteria, the preventive effect of bacterial alternation caused by continuous administration of antibiotics, and the inhibitory effect of carcinogenic substances have been clarified. ("Microorganisms", Volume 1, 4
No. 2, page 1987, Medical Publishing Center). in this way,
Bifidobacterium is closely related to the health of mammals including humans, and is attracting attention in the medical field.

As a method of growing Bifidobacterium in the intestine, there are a direct method of orally administering Bifidobacterium and a method of administering a substance used for Bifidobacterium (hereinafter referred to as Bifidobacterium). There is an indirect method of growing fidobacteria. The direct method has problems such as low stability of the Bifidobacterium preparation and a low colonization rate of the bacteria after administration, and the recent indirect method has been emphasized.

Conventionally, as bifidogenic factors, N-acetylglucosamine, derivatives thereof, carrot extract (mainly containing pantethine), lactulose, raffinose, stachyose, maltotriose ("Bifidobacterium", 77
Page, 1979, Yakult Honsha Co., Ltd., fructooligosaccharides ("Chemistry and Biology", 21, 291 pages, 1983, Gakkai Shuppan Center), galactooligosaccharides (JP-B-58-20266, JP-B-61-46479) JP, JP-A-60-41449), isomaltooligosaccharides (published by the Japanese Society of Nutrition and Food Science, 19
1986), cyclodextrin (JP-A-57-138385), konjac mannan (“RIKEN intestinal flora symposium III, intestinal flora and nutrition”, p. 89, 1983)
Soy Milk (JP-B-45-9822, JP-A-51-142566, JP-A-55-85390), soymilk extract (JP-A-59-17906), Various substances such as an extract of a non-pathogenic Escherichia coli culture (Japanese Patent Publication No. 50-13359) are known.

Hitherto, it has not been known that evening syrup promotes the growth of Bifidobacterium and suppresses the growth of intestinal harmful bacteria.

PROBLEM TO BE SOLVED BY THE INVENTION Since the above-mentioned known bifidobacterium is used in vivo for other intestinal bacteria such as lactic acid bacteria, the growth effect of bifidobacteria is not sufficient, and the method for producing them is not sufficient. However, there are drawbacks such as complexity and cost. Therefore, if there is a sufficient bifidobacterium growth promoting effect and intestinal harmful bacterial growth inhibitory effect, and a bifidobacterium which can be easily produced, it is extremely useful in industry.

Means for Solving the Problems Some of the present inventors have already conducted intensive studies on the bifidobacterium, and as a result, the evening meal obtained by processing the evening fruit selectively and remarkably promoted the growth of Bifidobacterium bacteria. (Japanese Patent Application No. 62-123761). The present inventors have found that evening syrup has a higher effect of promoting the growth of bifidobacteria than evening food, and completed the present invention.

The bifidobacterium growth promoter of the present invention contains evening syrup as an active ingredient.

What is evening syrup? (1) Evening fruit, that is, squeezed juice obtained from fruit without skin, cotton or whole fruit,
(2) Evening food, that is, the evening flesh is strip-shaped, chip-shaped,
Extracts extracted from powdered dried matter using an organic solvent mixed with water and water such as alcohols and acetone at an arbitrary ratio. (3) These squeezed liquids or extracts are concentrated and sterilized. Was done or not done.

The sterilized growth promoter of the present invention has a vapor pressure of 0.2 to 0.4.
kg / cm ^2, steam sterilization or alcohol addition of sterilizing time 5-20 min amount 15~150g / kg (gourd dry matter) can be produced by alcohol sterilization sterilizing days 1-30 days. This sterilized growth promoter has a general viable cell count of 5 × 10 ³ / g or less,
Escherichia coli group and Salmonella are negative, water content is 8% or less, and it is extremely safe for food hygiene.

In the case of unsterilized dried evening product, use alcohols for extraction or at 100 ° C, 3 for water extraction.
Sterilization can be achieved by heat treatment for 0 minutes.

The extraction solvent is usually used in a volume of 5 to 10 times the weight of the dried evening product, and is extracted at room temperature or with heating while stirring, and the insoluble matter is removed by filtration to obtain a filtrate. The obtained filtrate is used as it is or after being concentrated and used as evening syrup. This can be sterilized by a membrane or sterilized by heating to make a more hygienic and safe syrup.

The evening syrup of the present invention may be used as a syrup as a single component, or may be a drink (content liquid) or a solid food to which an additive is added.

Examples of additives include sweet corn, carrot powder, corn fiber, apple fiber,
Fiber components such as pumpkin powder, alginic acid, guar gum, gum arabic, pectin, polydextrose, excipients such as lactose, dextrin, starch, sucrose, glucose, fructose, sorbitol, mannitol, reduced maltose, and sweet components such as maltitol, Nutritional supplements such as milk powder, meat extract, liver hydrolyzate, royal jelly, carrot extract, binders such as polyvinylpyrrolidone, hydroxypropylcellulose, sodium carboxymethylcellulose, citric acid, malic acid, hydrochloric acid, sodium citrate, etc. Buffer components, polyoxyethylene hydrogenated castor oil 60, polyoxyl stearate 40, surfactants such as glycerin fatty acid ester, polyethylene glycol, sucrose fatty acid ester, propylene glycol, stearic acid Lubricants such as magnesium, calcium stearate, Lubri wax, talc, synthetic aluminum silicate, ascorbic acid, riboflavin,
And its derivatives, pyridoxine phosphate, nicotinic acid amide, calcium pantothenate, biotin, thiamine hydrochloride and other vitamins, flavoring agents, coloring agents, stabilizers, preservatives, etc. to promote the growth of Bifidobacterium bacteria・
What is necessary is just to select and use suitably according to the shape and / or taste of an intestinal harmful bacteria growth inhibitor.

The bifidobacterium growth promotion / intestinal harmful bacteria growth inhibitor of the present invention has a sweet taste and can be eaten as it is.
It can be eaten after being processed into drinks, powders, granules, and tablets, or added to soup stock, confectionery, noodles, tofu, and the like.

Action The action of the bifidobacterium growth promotion / intestinal harmful bacteria growth inhibitor of the present invention will be described.

As a sample, the evening syrup produced by the same method as in Production Example 1, the evening granule produced by the same method as Production Example 2, or the sterilized evening syrup produced by the same method as Production Example 4 was used. A known bifid factor was used as a control.

Test 1 Using a Negishi agar medium (Bacteriological Journal of Japan, Vol. 13, pp. 519, 1958) as a basal medium for searching for bifid factors, adding 0.5% of the sample (syrup is equivalent to powder) to a 9 cm petri dish Bifidobacterium longum (Bifidobacterium lo
ngum, ATCC 15707). This was cultured at 37 ° C. for 21 hours, and the growth state of the bacterium was observed. Table 1 shows the results.

In the table, ± in the column of the growth state indicates that the growth state is good, + indicates that the growth state is better than ±, and the larger the number, the better the growth state .

As is evident from Table 1, the evening syrup showed a better bifidobacterium growth-promoting hardening than the conventional bifidobacterium factor and evening powder.

Test 2 Evening syrup and evening powder were added to a basic medium, Tomarelli modified medium (Journal of Biochemical Chemistry, 181: 879, 1949).
２2% (syrup was converted to powder), and Bifidobacterium longum (ATCC 15707) was cultured at 37 ° C. for 16 hours. After the culture, the culture solution was applied to a BL agar medium, cultured at 37 ° C. for 48 hours, and the number of bacteria was measured. Table 2 shows the results.

As is clear from Table 2, the evening syrup was 10 times higher than Bifidobacterium at a concentration of 0.05% (in terms of powder).
0.10 to 1.00% shows a growth promoting effect of 100 to 10000 times, and is more potent than evening powder.

Test 3 A sterilized evening syrup and evening powder were used as samples.

Each sample was added to a modified Tomarellii medium (syrup was converted to powder), and Bifidobacterium longum (ATCC 1
5707) was cultured at 37 ° C. for 16 hours. After the culture, the culture solution was applied to a BL agar medium, cultured at 37 ° C. for 48 hours, and the number of bacteria was measured. Table 3 shows the results.

As is clear from Table 3, the sterilized evening syrup significantly promoted the growth of Bifidobacterium bacteria more than evening powder and intestinal harmful bacteria such as enterococci, Pseudomonas aeruginosa, Escherichia coli and Welsh bacteria. Rather, it exhibits a growth inhibitory effect on these bacteria.

Production Example 1 The outer skin of the evening face was thinly peeled, and the pulp was sliced into a thin strip.
After drying for 1 day in the sun, dry at 70 ° C for 5 hours to obtain a water content of about
2.3% of zonal evening food was obtained. 35 kg of this dried product was pulverized with a pulverizer (manufactured by Hosokawa Iron Works) to obtain 29 kg of evening powder.

Put 25 kg of this powder in a kettle, 160 l of ethyl alcohol and water
40 l were added, and the mixture was stirred at room temperature for about 1 hour and filtered. The filtrate was concentrated under reduced pressure at 50 ° C. to obtain 9.8 kg of syrup. The water content of this syrup was 19.2% as measured by the Karl Fischer method. The number of general viable bacteria was 34 / g, and coliforms and Salmonella were negative.

Production Example 2 380 g of the evening syrup obtained in Production Example 1, 500 g of crystalline cellulose, 150 g of corn starch and 30 g of hydroxypropyl cellulose were mixed in a mixing stirrer for 10 minutes, and then dried with hot air. This was allowed to cool, and then granulated with an oscillator (manufactured by Kikusui Seisakusho) to obtain 945 g of evening face granules.

Production Example 3 After mixing 895 g of the evening powder granules obtained in Production Example 2 and 5 g of magnesium stearate in a mixing stirrer for 1 minute, the mixture was tableted to obtain 6800 tablets of 100 mg of evening meal tablets of 100 mg each.

Production Example 4 35 kg of band-shaped evening food obtained in the same manner as in Production Example 1 was 0.2 kg /
It was sterilized 20 minutes in a steam pressure of cm ^2, moisture content of about 2.5%
It was dried with hot air at 60 ° C until the temperature became. Then, the same operation as in Production Example 1 was performed to obtain 28 kg of sterilized powdery evening meal.

Put 25kg of this powder in a kettle and 160l of isopropyl alcohol
And 40 l of water, and the mixture was stirred at 40 ° C. for about 1 hour, and then filtered. The obtained filtrate was concentrated under reduced pressure at 50 ° C.
kg gained. The water content of this syrup was measured by the Karl Fischer method to be 15.2%. The general viable count is
6.3 × 10 ² cells / g, E. coli and Salmonella were negative.

Production Example 5 2.7 kg of ethanol was sprayed on 30 kg of the band-shaped evening glory food obtained in the same manner as in Production Example 1 (90 g / kg band-shaped evening glory food), sealed and allowed to stand at room temperature for 5 days to form a chip of about 2 cm. 70 ℃
For 4 hours to obtain 25 kg of evening face chips. Put this in a kettle, add 170 l of water and 30 l of ethanol and heat to 60 ° C,
After stirring for 1 hour, the chips were filtered. The filtrate was concentrated under reduced pressure at 50 ° C. to obtain 8.0 kg of syrup. The water content of this syrup was 16.0%, the number of general viable bacteria was 30 cells / g, and coliform bacteria and Salmonella were negative.

Production Example 6 After the outer skin of the evening face was peeled thinly, the whole pulp was sliced to a thickness of about 2 cm, pressed, and centrifuged (4000 G) to obtain an evening face squeezed liquid. 100 l of this squeezed liquid was concentrated at 50 ° C. under reduced pressure to obtain 4.3 kg of syrup. The water content of this syrup was 14.6% as measured by the Karl Fischer method. The number of general viable bacteria was 13 / g, and coliforms and Salmonella were negative.

Example 5 To 5 healthy adults, 2 g / day of evening powder produced in the same manner as in Production Example 1 for 7 days, and 4 g of evening powder granules produced in the same manner as in Production Example 7 for 7 days. Each was administered before meals. On day 7 of each administration, the feces of each individual were collected,
The numbers of Bifidobacterium, lactic acid bacteria and Escherichia coli cultured and grown on a BS medium were measured, and the average value was determined. Table 4 shows the results.

As is clear from Table 4, the evening powder and the evening granules were found to selectively and significantly promote the growth of Bifidobacterium in the intestine.

Effect of the Invention The inhibitor of the present invention for promoting the growth of Bifidobacterium bacteria and inhibiting the growth of intestinal harmful bacteria selectively and significantly promotes the growth of Bifidobacterium bacteria, and suppresses the growth of intestinal harmful bacteria. In addition to helping maintain health, it is also expected as a drug such as an immunostimulant or an anticancer drug. Further, the evening syrup of the present invention is effective in a smaller amount than conventional evening powder, and since it is dissolved in water, it can be easily added to drinks, liquid confectioneries, and foods.

Claims (1)

(57) [Claims] 1. A bifidobacterium growth promoter which comprises a syrup-like substance extracted from evening grape fruit as an active ingredient and suppresses the growth of intestinal harmful bacteria.