JPH02135088A - Agent for promoting proliferation of bifido-bacterium and suppressing proliferation of noxious enterobacter - Google Patents

Abstract

PURPOSE:To obtain the subject agent effective in selectively and remarkably promoting the proliferation of bifidobacterium and suppressing the proliferation of noxious enterobacter, useful for the maintenance of health and producible at a low cost by using a syrupy substance extracted from the fruit of bottle gourd as an active component. CONSTITUTION:The objective agent is prepared by using a syrupy substance extracted from the fruit of bottle gourd as an active component. The syrupy substance is produced e.g., by extracting 1 pt.wt. of a dried bottle gourd fruit with 5-10 pts.wt. of an organic solvent, removing insoluble materials from the extract by filtration and concentrating the filtrate.

Description

[Detailed Description of the Invention] Industrial Field of Application The present invention relates to a syrup-like substance (hereinafter referred to as
It is called Yugao syrup. The present invention relates to an agent for promoting the growth of Bifidobacterium and inhibiting the growth of harmful intestinal bacteria, which contains Btfidobacterium as an active ingredient.

Conventional technology Yugao is an annual vine belonging to the Cucurbitaceae family, and its fruit is oval in shape, about 70 centimeters in diameter. Ganhong is made by peeling the pulp of this fruit into long, string-like pieces and drying them.

Bifidobacterium is nonpathogenic and mainly exists in the intestines of mammals including humans, and has been recognized to have physiological significance such as suppressing intestinal putrefaction products and improving diarrhea and constipation. It is a useful bacteria.

In recent years, the immune system-promoting effect of Bifidobacterium bacteria, the prevention effect of bacterial replacement that occurs when antibiotics are continuously administered, and the effect of suppressing carcinogenic substances have been revealed, and clinical applications are increasing. (Microorganisms, Vol. 1, No. 4, p. 2, 1987, Medical Publishing Center). As described above, Phytobacterium is deeply involved in the health of mammals including humans, and is attracting attention in the medical field.

There are two ways to grow Bifidobacterium in the intestines: the direct method of orally administering Bifidobacterium, and the method of administering a substance used by Bifidobacterium (hereinafter referred to as Bifidobacterium). There is an indirect method of growing Fidobacterium. Direct methods have problems such as low stability of Bifidobacterium preparations and low bacterial colonization rates after administration, so indirect methods have recently been emphasized.

Conventionally, bifidus factors include N-acetylglucosamine, its derivatives, carrot extract (mainly containing pantethine), lactulose, fufinose, stachyose, maltotriose ("Bifidobacterium", 77
Page, 1979, Yakult Honsha Co., Ltd.), Fructooligosaccharides (Chemistry and Biology, Vol. 21, p. 291, 1983
(2013, Gakkai Publishing Center), Galactooligosaccharides (Special Publications, 1973)
8-20266, Japanese Patent Publication No. 61-46479, Japanese Patent Application Laid-open No. 60-41449), isomalto-oligosaccharide (published by the Japan Society of Nutrition and Food Science, 1986), cyclodextrin (Japanese Patent Publication No. 57-138385) , Konnyaku Mannan (“RIKEN Intestinal Flora Symposium■
, "Intestinal Flora and Nutrition", p. 89, 1983, Gakkai Publishing Center), soy milk (Japanese Patent Publication No. 45-9822, JP-A-51-142566, JP-A-55-8539)
0), soy milk extract (Japanese Patent Publication No. 59-17906), extract of non-pathogenic Escherichia coli culture solution (Japanese Patent Publication No. 59-17906),
Various substances are known, such as No. 3359).

Conventionally, it has not been known that Yugao syrup promotes the growth of Bifidobacterium or inhibits the growth of harmful intestinal bacteria.

Problems that Ming is trying to solve The previously mentioned known bifidus factors are used by other intestinal bacteria such as lactic acid bacteria in vivo, so they do not have a sufficient growth effect on Bifidobacterium, and their production method It has drawbacks such as being complicated and expensive. Therefore, a bifidus factor that has a sufficient effect of promoting the growth of bifidobacteria and suppressing the growth of harmful intestinal bacteria and can be easily produced would be extremely useful industrially.

Means for Solving the Problems Some of the present inventors have already conducted intensive research on the bifidus factor, and have found that Yugao food processed from Yugao fruit selectively and significantly promotes the growth of Bifidobacterium bacteria. (Japanese Patent Application No. 123761/1982). We have now completed the present invention by discovering that Yugao syrup has a higher effect on promoting the growth of bifidobacteria than Yugao food.

The Bifidobacterium growth promoter of the present invention contains Yugao syrup as an active ingredient.

What is Yugao syrup? (1) Yugao fruit, i.e. the fruit with the skin removed, the juice obtained from the pith or the whole fruit; (2)
) Yugao food, that is, an extract extracted from dried Yugao fruit pulp in the form of strips, chips, or powder using an organic solvent mixed with water in any ratio such as water, alcohol, or acetone; (3) It is a concentrated juice or extract, and may or may not be sterilized.

The sterilized growth promoter of the present invention has a vapor pressure of 0.2 to 0.
．． 4kMCm2, sterilization time 5-20 minutes steam sterilization or alcohol addition amount 15-150q/ka (Yugao dried product)
It can be produced by alcohol sterilization for 1 to 30 days. This sterilized growth promoter has a general viable cell count of 5×10 3 cells/q or less, is negative for coliform bacteria and salmonella, and has a water content of 8% or less, making it extremely safe in terms of food hygiene.

In the case of unsterilized dried Yugao, by using alcohol during extraction, or by water extraction at 100℃,
It can be sterilized by heat treatment for 30 minutes.

The extraction solvent is usually used in a volume 5 to 10 times the weight of the dried Yugao, extracted at room temperature or heated with stirring, and suspicious substances are removed by filtration to obtain a filtrate.

The obtained filtrate is used as it is or after being concentrated as Yugao syrup. This can be sterilized or heat sterilized to produce a more hygienically safe syrup.

The Yugao syrup of the present invention may be used as a single syrup component, or may be used as a drink (oral liquid) or solid food with additives added.

Examples of additives include fiber components such as sweet corn, carrot powder, corn fiber, apple fiber, pumpkin powder, alginic acid, guar gum, gum arabic, pectin, and polydextrose; excipients such as lactose, dextrin, and starch; white sugar, glucose,
Sweet ingredients such as fructose, sorbitol, mannitol, reduced maltose, maltitol, milk powder, meat extract,
Nutritional supplements such as thawed liver, royal jelly, and carrot extract; binders such as polyvinylpyrrolidone, hydroxypropyl cellulose, and sodium carboxymethyl cellulose; buffer components such as citric acid, malic acid, hydrochloric acid, and sodium citrate; and polyoxyethylene. Surfactants such as hydrogenated castor oil 60, polyoxyl stearate 40, glycerin fatty acid ester, polyethylene glycol, sucrose fatty acid ester, propylene glycol, and lubricants such as magnesium stearate, calcium stearate, love wax, talc, and synthetic aluminum silicate. Examples include brighteners, vitamins such as ascorbic acid, riboflavin, and their derivatives, pyridoxine phosphate, nicotinic acid amide, calcium pantothenate, biotin, and thiamine hydrochloride, flavoring agents, coloring agents, stabilizers, and preservatives. , Bifidobacterium growth-promoting/intestinal harmful bacteria growth-inhibiting agent may be appropriately selected and used depending on the shape and/or preference.

The Bifidobacterium growth-promoting/intestinal harmful bacteria growth-inhibiting agent of the present invention has a sweet taste and can be eaten as it is, or processed and eaten in the form of drinks, powder, granules, tablets, or as a soup base. It can be added to sweets, noodles, tofu, etc.

Effects The effects of the Bifidobacterium growth-promoting/intestinal harmful bacteria growth-inhibiting agent of the present invention will be explained.

As samples, Yugao syrup produced in the same manner as in Production Example 1, Yugao granules produced in the same manner as in Production Example 2, or sterilized Yugao syrup produced in the same manner as in Production Example 4 were used. A known bifidus factor was used as a control.

Test 1 Plate agar medium as a basic medium for bifidus factor search (Japanese Journal of Bacteriology, Vol. 13, p. 519, 1958)
Add 0.5% of the sample to this (syrup is equivalent to powder), and add Bifidobacterium longum (Bifidobacterium longum) to a 9cm petri dish so that the number of colonies is approximately 50.
obacterium Ionaum, ATCC
15707) was applied. This was cultured at 37°C for 21 hours, and the state of growth of the bacteria was observed. The results are shown in Table 1.

As is clear from Table 1, Yugao syrup was found to have a better effect of promoting the growth of bifidobacteria than conventional bifidus factors and Yugao powder.

Test 2 Tomarelli medium (Journal of Biochemical Chemistry, vol. 181, p. 879, 19
Add 0 to 2% syrup to (49 years)), Bifidobacterium longum (^TCC15707)
was cultured at 37°C for 16 hours. After culturing, the culture solution was applied to a BL agar medium, and after culturing at 37°C for 48 hours, the number of bacteria was measured. The results are shown in Table 2.

(Leaving space below) In the table, 〇 in the proliferation status column indicates that the proliferation status is good, and 〇 indicates that the proliferation status is better than 〇.
The larger the number, the better the proliferation state.

Table 2 Each sample was added to Tomarelli medium (syrup is equivalent to powder), Bifidobacterium longum (ATCC15
707) was cultured at 37°C for 16 hours. After culturing, the culture solution was applied to a BL agar medium, and after culturing at 37°C for 48 hours, the number of bacteria was measured. The results are shown in Table 3.

(Left below) As is clear from Table 2, Yugao syrup has 10% effectiveness against Bifidobacterium at a concentration of 0.05% (powder equivalent).
times, 100 to i ooo at 0.10 to 1.00%.

It shows double the growth promoting effect and is more powerful than Yugao powder.

Exam 3 Sterilized Yugao syrup and Yugao powder were used as samples.

As is clear from Table 3, sterilized Yugao syrup is
It promotes the growth of Bifidobacterium more significantly than Yugao powder, and is not used by enterococcus, Pseudomonas aeruginosa, Escherichia coli, Clostridium perfringens, and other harmful intestinal bacteria, but rather inhibits the growth of these bacteria. Show effectiveness.

Production example 1 Peel the outer skin of Yugao thinly, slice the pulp into thin strips,
After drying in the sun, it was dried at 70'C for 5 hours to obtain a strip-shaped Yugao food with a moisture content of 2.3%. 35k of this dried material (
J was crushed with a pulverizer (manufactured by Line Iron Works) to obtain Yugao Powder 29k (J).

Put 25 kg of this powder into a pot and add 160 ml of ethyl alcohol.
1 and 401 parts of water were added, stirred at room temperature for about 1 hour, and then filtered. The filtrate was concentrated under reduced pressure at 50°C to form a syrup.9.
I got akg. The moisture content of this syrup was measured by the Chiruff-Ischer method and found to be 19.2%. - The number of semi-viable bacteria was 34/Ω, and coliform bacteria and salmonella were negative.

Production Example 2 Yugao Syrup 380 Q obtained in Production Example 1, 500 Cl of crystalline cellulose, 150 g of corn starch and 30 Cl of hydroxypropyl cellulose were mixed in a mixer for 100 minutes, and then dried with hot air.

After cooling this, it was sized with an oscillator (manufactured by Kikusui Seisakusho) to obtain Yugao granules 945q.

Production Example 3 After mixing 895 g of Yugao granules obtained in Production Example 2 and 5 q of magnesium stearate in a mixer for 1 minute,
The mixture was compressed to obtain 6,800 Yugao tablets each weighing 100 mg.

Production Example 4 35 kq of the strip-shaped Yugao food obtained in the same manner as Production Example 1 was mixed with 0.
After being sterilized for 20 minutes under a steam pressure of 2 kMCm2, it was dried with hot air at 60°C until the moisture content was about 2.5%.

Then, this was operated in the same manner as in Production Example 1 to obtain 28 kg of sterilized powdered Yugao food.

Put 25kg of this powder into a pot and add 16g of isopropyl alcohol.
01 and water 401 were added, stirred at 40°C for about 1 hour, and then filtered. The obtained filtrate was concentrated under reduced pressure at 50'C to obtain 11.6 kg of syrup. The moisture content of this syrup was measured by the Karl Fischer method and was found to be 15.2%.
Met. - The semi-viable bacterial count was 6.3 x 102 cells/q, and E. coli and Salmonella were negative.

Production Example 5 2.7 kg of ethanol was sprayed onto 3 kg of Yugao strips obtained in the same manner as in Production Example 1 (90q/ko Yugao strips), sealed and left at room temperature for 5 days, and then shaped into chips of about 2 cm. Dry at 70'C for 4 hours to get Yugao Chip 25! I got II. This was placed in a pot, 1701 parts of water and 30 parts of ethanol were added, heated to 60°C, stirred for 1 hour, and then the chips were filtered. The filtrate was concentrated under reduced pressure at 50°C to form a syrup.8. I got OK. The moisture content of this syrup is 16
,0%, -Semi-viable bacteria count was 30/q, coliform and salmonella were negative.

Production Example 6 The outer skin of Ta-gao was peeled off, and the whole pulp was sliced into rings about 2 cm thick, then squeezed and centrifuged (4000G) to obtain Yu-gao juice. This squeezed liquid 1001 was concentrated under reduced pressure at 50°C to obtain 4.3 kg of syrup. When the moisture content of this syrup was measured using the Karl Fischer method, it was found to be 14.6
%Met. - The number of semi-viable bacteria was 13/Ω, and coliform and salmonella were negative.

Example Five healthy adults were given 2 q/day of Yugao powder produced in the same manner as Production Example 1 for 7 days, and 4 q/day of Yugao granules produced in the same manner as Production Example 2 for 7 days. Each was administered before meals. On the 7th day after each administration, feces from each person was collected, cultured on BS medium, and the number of grown Bifidobacteria, lactic acid bacteria, and E. coli was measured, and the average value was determined.

The results are shown in Table 4.

(Margin below) Table 4 In the table A: Yugao powder B: Yugao granules Bacterium: Bifidobacterium As is clear from Table 4, Yugao powder and Yugao granules select the growth of Bifidobacterium in the intestines. specifically, and
It was found that this significantly promoted

The growth inhibitor of endogenous harmful bacteria selectively and significantly promotes the growth of Phytobacterium bacteria and suppresses the growth of harmful intestinal bacteria, so it is useful for maintaining health, and is also used as an immunostimulant, anticancer agent, etc. is also expected. Moreover, the Yugao syrup of the present invention is more effective in a smaller amount than the conventional Yugao powder, and since it dissolves in water, it can be easily added to drinks, liquid sweets, and foods.

Patent applicant: Tokyo Tanabe Pharmaceutical Co., Ltd.

Claims (1)

[Claims] (1) An agent for promoting the growth of Bifidobacterium and inhibiting the growth of harmful intestinal bacteria, the active ingredient being a syrup-like substance extracted from Yugao fruit.