JPH02135088A - Agent for promoting proliferation of bifido-bacterium and suppressing proliferation of noxious enterobacter - Google Patents

Agent for promoting proliferation of bifido-bacterium and suppressing proliferation of noxious enterobacter

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Publication number
JPH02135088A
JPH02135088A JP63288928A JP28892888A JPH02135088A JP H02135088 A JPH02135088 A JP H02135088A JP 63288928 A JP63288928 A JP 63288928A JP 28892888 A JP28892888 A JP 28892888A JP H02135088 A JPH02135088 A JP H02135088A
Authority
JP
Japan
Prior art keywords
yugao
proliferation
syrup
growth
bifidobacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP63288928A
Other languages
Japanese (ja)
Other versions
JP2698128B2 (en
Inventor
Yasuhiko Kikuchi
菊池 靖彦
Takuro Yamaura
山浦 卓郎
Shinzo Nakazawa
中澤 進三
Minoru Uchida
実 内田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tokyo Tanabe Co Ltd
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Tokyo Tanabe Co Ltd
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Filing date
Publication date
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Priority to JP63288928A priority Critical patent/JP2698128B2/en
Publication of JPH02135088A publication Critical patent/JPH02135088A/en
Application granted granted Critical
Publication of JP2698128B2 publication Critical patent/JP2698128B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

PURPOSE:To obtain the subject agent effective in selectively and remarkably promoting the proliferation of bifidobacterium and suppressing the proliferation of noxious enterobacter, useful for the maintenance of health and producible at a low cost by using a syrupy substance extracted from the fruit of bottle gourd as an active component. CONSTITUTION:The objective agent is prepared by using a syrupy substance extracted from the fruit of bottle gourd as an active component. The syrupy substance is produced e.g., by extracting 1 pt.wt. of a dried bottle gourd fruit with 5-10 pts.wt. of an organic solvent, removing insoluble materials from the extract by filtration and concentrating the filtrate.

Description

【発明の詳細な説明】 産業上の利用分野 本発明は夕顔果実から抽出したシラップ状物質(以下、
夕顔シラップという。)を有効成分とするビフィドバク
テリウム菌(Btfidobacterium )の増
殖促進および腸内有害菌の増殖抑制剤に関する。
[Detailed Description of the Invention] Industrial Field of Application The present invention relates to a syrup-like substance (hereinafter referred to as
It is called Yugao syrup. The present invention relates to an agent for promoting the growth of Bifidobacterium and inhibiting the growth of harmful intestinal bacteria, which contains Btfidobacterium as an active ingredient.

従来の技術 夕顔はうり科の一年生つる草で、その果実は直径70セ
ンチ程の長円形になる。この果実の果肉を紐のように細
長くむいて乾燥したものが干瓢である。
Conventional technology Yugao is an annual vine belonging to the Cucurbitaceae family, and its fruit is oval in shape, about 70 centimeters in diameter. Ganhong is made by peeling the pulp of this fruit into long, string-like pieces and drying them.

ビフィドバクテリウム菌は病原性がなく、ヒトを始めと
して、主に哺乳動物の腸内に存在し、腸内腐敗生成物の
抑制、下痢、便秘症の改善などの生理的意義が認められ
ている有用な菌である。
Bifidobacterium is nonpathogenic and mainly exists in the intestines of mammals including humans, and has been recognized to have physiological significance such as suppressing intestinal putrefaction products and improving diarrhea and constipation. It is a useful bacteria.

近年、ビフィドバクテリウム菌の免疫力促進効果や抗生
物質を連続投与した時に起る菌交代症の防止効果、さら
には発癌性物質の抑制効果などが明らかになり、臨床へ
の応用が盛んに行われている(「微生物」、1巻、4号
、2頁、1987年、医学出版センター)。このように
、ごフィトバクテリウム菌は、ヒトを始めとする哺乳動
物の健康に深いかかわりを持ち、医療の分野において注
目されている。
In recent years, the immune system-promoting effect of Bifidobacterium bacteria, the prevention effect of bacterial replacement that occurs when antibiotics are continuously administered, and the effect of suppressing carcinogenic substances have been revealed, and clinical applications are increasing. (Microorganisms, Vol. 1, No. 4, p. 2, 1987, Medical Publishing Center). As described above, Phytobacterium is deeply involved in the health of mammals including humans, and is attracting attention in the medical field.

ビフィドバクテリウム菌を腸内で増殖させる方法として
は、ビフィドバクテリウム菌を経口投与する直接法とビ
フィドバクテリウム菌に利用される物質(以下、ビフィ
ズスファクターという。)を投与してビフィドバクテリ
ウム菌を増殖させる間接法とがある。直接法ではビフィ
ドバクテリウム菌製剤の安定性が低い、投与後の菌の定
着率が低いなどの問題があり、最近は間接法が重視され
ている。
There are two ways to grow Bifidobacterium in the intestines: the direct method of orally administering Bifidobacterium, and the method of administering a substance used by Bifidobacterium (hereinafter referred to as Bifidobacterium). There is an indirect method of growing Fidobacterium. Direct methods have problems such as low stability of Bifidobacterium preparations and low bacterial colonization rates after administration, so indirect methods have recently been emphasized.

従来、ビフィズスファクターとしては、N−アセチルグ
ルコサミン、その誘導体、ニンジン抽出液(主にパンテ
ティンを含有)、ラクチュロース、フフィノース、スタ
キオース、マルトトリオース(「ビフィズス菌」、77
頁、1979年、株式会社ヤクルト本社)、フラクトオ
リゴ糖(「化学と生物」、21巻、291頁、1983
年、学会出版センター)、ガラクトオリゴ糖(特公昭5
8−20266号公報、特公昭61−46479号公報
、特開昭60−41449号公報)、イソマルトオリゴ
糖(日本栄養食料学会発表、1986年)、シクロデキ
ストリン(特開昭57−138385号公報)、コンニ
ャクマンナン(「理研腸内フローラ シンポジュウム■
、腸内フローラと栄養」、89頁、1983年、学会出
版センター)、豆乳(特公昭45−9822号公報、特
開昭51−142566号公報、特開昭55−8539
0号公報)、豆乳抽出物(特開昭59−17906号公
報)、非病原性大腸菌培養液の抽出液(特公昭50−1
3359号公報)などの種々の物質が知られている。
Conventionally, bifidus factors include N-acetylglucosamine, its derivatives, carrot extract (mainly containing pantethine), lactulose, fufinose, stachyose, maltotriose ("Bifidobacterium", 77
Page, 1979, Yakult Honsha Co., Ltd.), Fructooligosaccharides (Chemistry and Biology, Vol. 21, p. 291, 1983
(2013, Gakkai Publishing Center), Galactooligosaccharides (Special Publications, 1973)
8-20266, Japanese Patent Publication No. 61-46479, Japanese Patent Application Laid-open No. 60-41449), isomalto-oligosaccharide (published by the Japan Society of Nutrition and Food Science, 1986), cyclodextrin (Japanese Patent Publication No. 57-138385) , Konnyaku Mannan (“RIKEN Intestinal Flora Symposium■
, "Intestinal Flora and Nutrition", p. 89, 1983, Gakkai Publishing Center), soy milk (Japanese Patent Publication No. 45-9822, JP-A-51-142566, JP-A-55-8539)
0), soy milk extract (Japanese Patent Publication No. 59-17906), extract of non-pathogenic Escherichia coli culture solution (Japanese Patent Publication No. 59-17906),
Various substances are known, such as No. 3359).

従来、夕顔シラップがビフィドバクテリウム菌の増殖を
促進することおよび腸内有害菌の増殖を抑制することは
知られていない。
Conventionally, it has not been known that Yugao syrup promotes the growth of Bifidobacterium or inhibits the growth of harmful intestinal bacteria.

明が解決しようとする課題 前述の既知ビフィズスファクターは生体内で乳酸菌など
の他の腸内細菌にも利用されるので、ビフィドバクテリ
ウム菌の増殖効果が充分でなく、また、それらの製造方
法が繁雑である、高価であるなどの欠点がある。従って
、充分なビフィズス菌増殖促進効果、腸内有害菌増殖抑
制効果があり、しかも、容易に製造できるビフィズスフ
ァクターがあれば、産業上極めて有益である。
Problems that Ming is trying to solve The previously mentioned known bifidus factors are used by other intestinal bacteria such as lactic acid bacteria in vivo, so they do not have a sufficient growth effect on Bifidobacterium, and their production method It has drawbacks such as being complicated and expensive. Therefore, a bifidus factor that has a sufficient effect of promoting the growth of bifidobacteria and suppressing the growth of harmful intestinal bacteria and can be easily produced would be extremely useful industrially.

課題を解決するための手段 既に、本発明者らの一部はビフィズスファクターについ
て鋭意研究した結果、夕顔果実を加工した夕顔食品がビ
フィドバクテリウム菌の増殖を選択的に、かつ、顕著に
促進することを見出した(特願昭62−123761号
明細書)。今回、夕顔シラップに夕顔食品より高いビフ
ィズス菌増殖促進効果があることを見出し、本発明を完
成した。
Means for Solving the Problems Some of the present inventors have already conducted intensive research on the bifidus factor, and have found that Yugao food processed from Yugao fruit selectively and significantly promotes the growth of Bifidobacterium bacteria. (Japanese Patent Application No. 123761/1982). We have now completed the present invention by discovering that Yugao syrup has a higher effect on promoting the growth of bifidobacteria than Yugao food.

本発明のビフィドバクテリウム菌増殖促進剤は夕顔シラ
ップを有効成分とする。
The Bifidobacterium growth promoter of the present invention contains Yugao syrup as an active ingredient.

夕顔シラップとは(1)夕顔果実、すなわち、皮を除い
た果実、わたまたは果実全体から得られる搾り液、(2
)夕顔食品、すなわち、夕顔果肉を帯状、チップ状、粉
状にした乾燥物から水およびアルコール類、アセトン等
の水と任意の割合で混合する有機溶媒を用いて抽出した
抽出液、(3)それら搾り液または抽出液を濃縮したも
のであり、殺菌されたものまたはされていないものであ
る。
What is Yugao syrup? (1) Yugao fruit, i.e. the fruit with the skin removed, the juice obtained from the pith or the whole fruit; (2)
) Yugao food, that is, an extract extracted from dried Yugao fruit pulp in the form of strips, chips, or powder using an organic solvent mixed with water in any ratio such as water, alcohol, or acetone; (3) It is a concentrated juice or extract, and may or may not be sterilized.

本発明の、殺菌された増殖促進剤は、蒸気圧0.2〜0
.4kMCm2、殺菌時間5〜20分の蒸気殺菌または
アルコール添加量15〜150q/ka(夕顔乾燥物)
、殺菌日数1〜30日のアルコール殺菌により製造する
ことができる。この殺菌された増殖促進剤は一般生菌数
が5X103個/q以下、大腸菌群およびサルモネラが
陰性、水分含量が8%以下であり、食品衛生上極めて安
全である。
The sterilized growth promoter of the present invention has a vapor pressure of 0.2 to 0.
.. 4kMCm2, sterilization time 5-20 minutes steam sterilization or alcohol addition amount 15-150q/ka (Yugao dried product)
It can be produced by alcohol sterilization for 1 to 30 days. This sterilized growth promoter has a general viable cell count of 5×10 3 cells/q or less, is negative for coliform bacteria and salmonella, and has a water content of 8% or less, making it extremely safe in terms of food hygiene.

殺菌されていない夕顔乾燥物の場合、抽出時にアルコー
ル類を用いることにより、または水抽出では100℃、
30分間の加熱処理により殺菌することができる。
In the case of unsterilized dried Yugao, by using alcohol during extraction, or by water extraction at 100℃,
It can be sterilized by heat treatment for 30 minutes.

抽出溶媒は、通常、夕顔乾燥物重量の5〜10倍の容量
を用い、室温または加熱して、攪拌しながら抽出し、不
審物を濾過により除き、濾液を得る。
The extraction solvent is usually used in a volume 5 to 10 times the weight of the dried Yugao, extracted at room temperature or heated with stirring, and suspicious substances are removed by filtration to obtain a filtrate.

得られた濾液をそのまままたは濃縮して夕顔シラップと
して用いる。これをメンプラン除菌または加熱滅菌して
、より衛生上安全なシラップとすることとができる。
The obtained filtrate is used as it is or after being concentrated as Yugao syrup. This can be sterilized or heat sterilized to produce a more hygienically safe syrup.

本発明の夕顔シラップは、シラップ単一成分のままで用
いることはもちろん、添加剤を加えたドリンク剤(内用
液剤)または固形の食品としてもよい。
The Yugao syrup of the present invention may be used as a single syrup component, or may be used as a drink (oral liquid) or solid food with additives added.

添加剤としては、例えば、スィートコーン、キャロット
パウダー、コーンファイバー、アップルファイバー、か
ぼちゃ粉末、アルギン酸、グアガム、アラビアガム、ペ
クチン、ポリデキストロスなどの繊維成分、乳糖、デキ
ストリン、でんぷんなどの賦形剤、白糖、グルコース、
果糖、ソルビトール、マンニトール、還元麦芽糖、マル
チトールなどの甘味成分、ミルクパウダー、肉エキス、
肝氷解物、ローヤルゼリー、ニンジン抽出物などの栄養
補給剤、ポリビニルピロリドン、ヒドロキシプロピルセ
ルロース、カルボキシメチルセルロースナトリウムなど
の結合剤、クエン酸、リンゴ酸、塩酸、クエン酸ナトリ
ウム、などのバッファー成分、ポリオキシエチレン硬化
ヒマシ油60、ステアリン酸ポリオキシル40、グリセ
リン脂肪酸エステル、ポリエチレングリコール、ショ糖
脂肪酸エステル、プロピレングリコールなどの界面活性
剤、ステアリン酸マグネシウム、ステアリン酸カルシウ
ム、ラブリ・ワックス、タルク、合成ケイ酸アルミニウ
ムなどの滑沢剤、アスコルビン酸、リボフラビン、およ
びその誘導体、リン酸ピリドキシン、ニコチン酸アミド
、パントテン酸カルシウム、ビオチン、塩酸チアミンな
どのビタミン剤、着香剤、着色剤、安定化剤、防腐剤な
どが挙げられ、ビフィドバクテリウム菌増殖促進・腸内
有害菌増殖抑制剤の形状および/または嗜好に応じて適
宜選択して使用すればよい。
Examples of additives include fiber components such as sweet corn, carrot powder, corn fiber, apple fiber, pumpkin powder, alginic acid, guar gum, gum arabic, pectin, and polydextrose; excipients such as lactose, dextrin, and starch; white sugar, glucose,
Sweet ingredients such as fructose, sorbitol, mannitol, reduced maltose, maltitol, milk powder, meat extract,
Nutritional supplements such as thawed liver, royal jelly, and carrot extract; binders such as polyvinylpyrrolidone, hydroxypropyl cellulose, and sodium carboxymethyl cellulose; buffer components such as citric acid, malic acid, hydrochloric acid, and sodium citrate; and polyoxyethylene. Surfactants such as hydrogenated castor oil 60, polyoxyl stearate 40, glycerin fatty acid ester, polyethylene glycol, sucrose fatty acid ester, propylene glycol, and lubricants such as magnesium stearate, calcium stearate, love wax, talc, and synthetic aluminum silicate. Examples include brighteners, vitamins such as ascorbic acid, riboflavin, and their derivatives, pyridoxine phosphate, nicotinic acid amide, calcium pantothenate, biotin, and thiamine hydrochloride, flavoring agents, coloring agents, stabilizers, and preservatives. , Bifidobacterium growth-promoting/intestinal harmful bacteria growth-inhibiting agent may be appropriately selected and used depending on the shape and/or preference.

本発明のビフィドバクテリウム菌増殖促進・腸内有害菌
増殖抑制剤は甘味があり、そのまま食べられるほか、ド
リンク剤、粉状、顆粒状、錠剤の形に加工して食べるか
、スープの素、菓子、麺類、豆腐などに添加して食べる
ことができる。
The Bifidobacterium growth-promoting/intestinal harmful bacteria growth-inhibiting agent of the present invention has a sweet taste and can be eaten as it is, or processed and eaten in the form of drinks, powder, granules, tablets, or as a soup base. It can be added to sweets, noodles, tofu, etc.

作用 本発明のビフィドバクテリウム菌増殖促進・腸内有害菌
増殖抑制剤の作用について説明する。
Effects The effects of the Bifidobacterium growth-promoting/intestinal harmful bacteria growth-inhibiting agent of the present invention will be explained.

試料として、製造例1と同様の方法で製造した夕顔シラ
ップ、製造例2と同様の方法で製造した夕顔顆粒または
製造例4と同様の方法で製造した殺菌夕顔シラップを使
用した。対照として既知のビフィズスファクターを用い
た。
As samples, Yugao syrup produced in the same manner as in Production Example 1, Yugao granules produced in the same manner as in Production Example 2, or sterilized Yugao syrup produced in the same manner as in Production Example 4 were used. A known bifidus factor was used as a control.

試験1 ビフィズスファクター検索用基礎培地として板片寒天培
地(日本細菌学雑誌、13巻、519頁、1958年)
を用い、これに試料を0.5%添加しくシラップは粉末
換算量)、9cmシャーレにコロニー数が約50個とな
るようにビフィドバクテリウム ロングム(Bifid
obacterium  Ionaum 、 ATCC
15707)を塗布した。これを37℃で21時間培養
し、該菌の増殖状態を観察した。結果を表1に示す。
Test 1 Plate agar medium as a basic medium for bifidus factor search (Japanese Journal of Bacteriology, Vol. 13, p. 519, 1958)
Add 0.5% of the sample to this (syrup is equivalent to powder), and add Bifidobacterium longum (Bifidobacterium longum) to a 9cm petri dish so that the number of colonies is approximately 50.
obacterium Ionaum, ATCC
15707) was applied. This was cultured at 37°C for 21 hours, and the state of growth of the bacteria was observed. The results are shown in Table 1.

表1から明らかなように、夕顔シラップは従来のビフィ
ズスファクターおよび夕顔粉末よりも優れたビフィズス
菌増殖促進効果が認められた。
As is clear from Table 1, Yugao syrup was found to have a better effect of promoting the growth of bifidobacteria than conventional bifidus factors and Yugao powder.

試験2 夕顔シラップおよび夕顔粉末を基礎培地であるトマレリ
(TOmarelli )培地(ジャーナル オブバイ
オケミカル ケミストリー、181巻、879頁、19
49年)に0〜2%添加しくシラップは粉末換算)、ビ
フィドバクテリウム ロングム(^TCC15707)
を37℃で16時間培養した。培養後、培養液をBL寒
天培地に塗布し、37℃で48時間培養した後、菌数を
測定した。結果を表2に示す。
Test 2 Tomarelli medium (Journal of Biochemical Chemistry, vol. 181, p. 879, 19
Add 0 to 2% syrup to (49 years)), Bifidobacterium longum (^TCC15707)
was cultured at 37°C for 16 hours. After culturing, the culture solution was applied to a BL agar medium, and after culturing at 37°C for 48 hours, the number of bacteria was measured. The results are shown in Table 2.

(以下余白) 表中、増殖状態の欄の士は増殖状態が良好であることを
示し、十は士よりも増殖状態が良好であることを示し、
その数が多い程、増殖状態が良好であることを示す。
(Leaving space below) In the table, 〇 in the proliferation status column indicates that the proliferation status is good, and 〇 indicates that the proliferation status is better than 〇.
The larger the number, the better the proliferation state.

表2 各々の試料をトマレリ培地に添加しくシラップは粉末換
算)、ビフィドバクテリウム ロングム(ATCC15
707)を37°Cで16時間培養した。培養後、培養
液をBL寒天培地に塗布し、37℃で48時間培養した
後、菌数を測定した。結果を表3に示す。
Table 2 Each sample was added to Tomarelli medium (syrup is equivalent to powder), Bifidobacterium longum (ATCC15
707) was cultured at 37°C for 16 hours. After culturing, the culture solution was applied to a BL agar medium, and after culturing at 37°C for 48 hours, the number of bacteria was measured. The results are shown in Table 3.

(以下余白) 度 表2から明らかなように、夕顔シラップはビフィドバク
テリウム菌に対し、濃度0.05%(粉末換算)で10
倍、0.10〜1.00%で100〜i ooo。
(Left below) As is clear from Table 2, Yugao syrup has 10% effectiveness against Bifidobacterium at a concentration of 0.05% (powder equivalent).
times, 100 to i ooo at 0.10 to 1.00%.

倍の増殖促進効果を示し、夕顔粉末よりも強力である。It shows double the growth promoting effect and is more powerful than Yugao powder.

試験3 試料として殺菌夕顔シラップおよび夕顔粉末を用いた。Exam 3 Sterilized Yugao syrup and Yugao powder were used as samples.

表3から明らかなように、殺菌された夕顔シラップは、
夕顔粉末よりもビフィドバクテリウム菌の増殖を著るし
く促進し、腸球菌、緑膿菌、大腸菌、ウェルシュ菌など
の腸内有害菌には利用されず、むしろ、これらの菌に対
し増殖抑制効果を示す。
As is clear from Table 3, sterilized Yugao syrup is
It promotes the growth of Bifidobacterium more significantly than Yugao powder, and is not used by enterococcus, Pseudomonas aeruginosa, Escherichia coli, Clostridium perfringens, and other harmful intestinal bacteria, but rather inhibits the growth of these bacteria. Show effectiveness.

製造例1 夕顔の外皮を薄く剥ぎ、果肉を薄帯状にスライスし、1
日入日乾燥した後、70’Cで5時間乾燥して水分含量
的2.3%の帯状夕顔食品を得た。この乾燥物35k(
Jをパルベライザ−(線用鉄工所製)で粉砕して夕顔粉
末29k(Jを得た。
Production example 1 Peel the outer skin of Yugao thinly, slice the pulp into thin strips,
After drying in the sun, it was dried at 70'C for 5 hours to obtain a strip-shaped Yugao food with a moisture content of 2.3%. 35k of this dried material (
J was crushed with a pulverizer (manufactured by Line Iron Works) to obtain Yugao Powder 29k (J).

この粉末25kgを釜に入れ、エチルアルコール160
1と水401とを加え、約1時間、室温で攪拌してから
濾過した。濾液を50℃で減圧濃縮して、シラップ9.
 akgを得た。このシラップの水分を力ルフ、イッシ
ャー法で測定したところ、19.2%であった。−半生
菌数は34個/Ω、大腸菌群およびサルモネラは陰性で
あった。
Put 25 kg of this powder into a pot and add 160 ml of ethyl alcohol.
1 and 401 parts of water were added, stirred at room temperature for about 1 hour, and then filtered. The filtrate was concentrated under reduced pressure at 50°C to form a syrup.9.
I got akg. The moisture content of this syrup was measured by the Chiruff-Ischer method and found to be 19.2%. - The number of semi-viable bacteria was 34/Ω, and coliform bacteria and salmonella were negative.

製造例2 製造例1で得られた夕顔シラップ380 Qと結晶セル
ロース500Cl、とうもろこしでんぷん150gおよ
びヒドロキシプロピルセルロース30C1とを混合攪拌
機中で100分間混した後、熱風乾燥した。
Production Example 2 Yugao Syrup 380 Q obtained in Production Example 1, 500 Cl of crystalline cellulose, 150 g of corn starch and 30 Cl of hydroxypropyl cellulose were mixed in a mixer for 100 minutes, and then dried with hot air.

これを放冷後、オシレーター(菊水製作所製)で整粒し
て夕顔顆粒945qを得た。
After cooling this, it was sized with an oscillator (manufactured by Kikusui Seisakusho) to obtain Yugao granules 945q.

製造例3 製造例2で得られた夕顔顆粒895gとステアリン酸マ
グネシウム5qとを混合攪拌機中で1分間混合した後、
打錠して1錠100mgの夕顔錠剤6800錠を得た。
Production Example 3 After mixing 895 g of Yugao granules obtained in Production Example 2 and 5 q of magnesium stearate in a mixer for 1 minute,
The mixture was compressed to obtain 6,800 Yugao tablets each weighing 100 mg.

製造例4 製造例1と同様にして得た帯状夕顔食品35kqを0.
2kMCm2の蒸気圧下で20分間殺菌した後、水分含
量が約2.5%になるまで60℃で熱風乾燥した。
Production Example 4 35 kq of the strip-shaped Yugao food obtained in the same manner as Production Example 1 was mixed with 0.
After being sterilized for 20 minutes under a steam pressure of 2 kMCm2, it was dried with hot air at 60°C until the moisture content was about 2.5%.

ついで、これを製造例1と同様に操作して殺菌された粉
状の夕顔食品28kgを得た。
Then, this was operated in the same manner as in Production Example 1 to obtain 28 kg of sterilized powdered Yugao food.

この粉末25kgを釜に入れイソプロピルアルコル16
01と水401とを加えて、40℃で約1時間攪拌した
後、濾過した。得られた濾液を50’Cで減圧濃縮して
シラップ11.6kgを得た。このシラップの水分をカ
ールフィッシャー法により測定したところ、15.2%
であった。−半生菌数は6.3X102個/q、大腸菌
およびサルモネラは陰性であった。
Put 25kg of this powder into a pot and add 16g of isopropyl alcohol.
01 and water 401 were added, stirred at 40°C for about 1 hour, and then filtered. The obtained filtrate was concentrated under reduced pressure at 50'C to obtain 11.6 kg of syrup. The moisture content of this syrup was measured by the Karl Fischer method and was found to be 15.2%.
Met. - The semi-viable bacterial count was 6.3 x 102 cells/q, and E. coli and Salmonella were negative.

製造例5 製造例1と同様にして得た帯状夕顔食品3okgにエタ
ノール2.7kgを噴霧しく90q/ko帯状夕顔食品
)、密封して室温で5日間放置した後、約2cmのチッ
プ状とし、70’Cで4時間乾燥して夕顔チップ25に
!IIを得た。これを釜に入れ、水1701とエタノー
ル301とを加えて60℃に加温し、1時間攪拌してか
らチップを濾過した。濾液を50°Cで減圧濃縮してシ
ラップ8. okaを得た。このシラップの水分は16
,0%、−半生菌数は30個/q、大腸菌群およびサル
モネラは陰性であった。
Production Example 5 2.7 kg of ethanol was sprayed onto 3 kg of Yugao strips obtained in the same manner as in Production Example 1 (90q/ko Yugao strips), sealed and left at room temperature for 5 days, and then shaped into chips of about 2 cm. Dry at 70'C for 4 hours to get Yugao Chip 25! I got II. This was placed in a pot, 1701 parts of water and 30 parts of ethanol were added, heated to 60°C, stirred for 1 hour, and then the chips were filtered. The filtrate was concentrated under reduced pressure at 50°C to form a syrup.8. I got OK. The moisture content of this syrup is 16
,0%, -Semi-viable bacteria count was 30/q, coliform and salmonella were negative.

製造例6 タ顔の外皮を薄く剥ぎ、果肉全体を約2Cmの厚さに輪
切りにした後、圧搾し、遠心分離(4000G )して
夕顔搾り液を得た。この搾り液1001を50℃で減圧
濃縮してシラップ4.3kgを得た。このシラップの水
分をカールフィッシャー法で測定したところ、14,6
%であった。−半生菌数は13個/Ω、大腸菌群および
サルモネラは陰性であった。
Production Example 6 The outer skin of Ta-gao was peeled off, and the whole pulp was sliced into rings about 2 cm thick, then squeezed and centrifuged (4000G) to obtain Yu-gao juice. This squeezed liquid 1001 was concentrated under reduced pressure at 50°C to obtain 4.3 kg of syrup. When the moisture content of this syrup was measured using the Karl Fischer method, it was found to be 14.6
%Met. - The number of semi-viable bacteria was 13/Ω, and coliform and salmonella were negative.

実施例 健康な成人5人に、製造例1と同様の方法で製造した夕
顔粉末を1日2qづつ7日間、および製造例2と同様の
方法で製造した夕顔顆粒を1日4qづつ7日間、それぞ
れ食前に投与した。各投与7日目に各人の糞便を採取し
、BS培地上で培養し、生育したビフィドバクテリウム
菌数、乳酸菌数および大腸菌数を測定し、その平均値を
求めた。
Example Five healthy adults were given 2 q/day of Yugao powder produced in the same manner as Production Example 1 for 7 days, and 4 q/day of Yugao granules produced in the same manner as Production Example 2 for 7 days. Each was administered before meals. On the 7th day after each administration, feces from each person was collected, cultured on BS medium, and the number of grown Bifidobacteria, lactic acid bacteria, and E. coli was measured, and the average value was determined.

結果を表4に示す。The results are shown in Table 4.

(以下余白) 表4 表中 A:夕顔粉末 B:夕顔顆粒 ビ菌:ビフィドバクテリウム菌 表4から明らかなように、夕顔粉末および夕顔顆粒は腸
内のビフィドバクテリウム菌の増殖を選択的に、かつ、
顕著に促進することが認められた。
(Margin below) Table 4 In the table A: Yugao powder B: Yugao granules Bacterium: Bifidobacterium As is clear from Table 4, Yugao powder and Yugao granules select the growth of Bifidobacterium in the intestines. specifically, and
It was found that this significantly promoted

内存害菌増殖抑制剤はごフィトバクテリウム菌の増殖を
選択的、かつ、顕著に促進し、腸内有害菌の増殖を抑制
するので、健康維持に役立つほか、免疫賦活剤、制癌剤
などの薬剤としても期待される。また、本発明の夕顔シ
ラップは、従来の夕顔粉末よりも少量で効果があり、ま
た、水に溶解するのでドリンク剤、液状の菓子類、食品
類への添加が容易である。
The growth inhibitor of endogenous harmful bacteria selectively and significantly promotes the growth of Phytobacterium bacteria and suppresses the growth of harmful intestinal bacteria, so it is useful for maintaining health, and is also used as an immunostimulant, anticancer agent, etc. is also expected. Moreover, the Yugao syrup of the present invention is more effective in a smaller amount than the conventional Yugao powder, and since it dissolves in water, it can be easily added to drinks, liquid sweets, and foods.

特許出願人 東京田辺製薬株式会社Patent applicant: Tokyo Tanabe Pharmaceutical Co., Ltd.

Claims (1)

【特許請求の範囲】[Claims] (1)夕顔果実から抽出したシラップ状物質を有効成分
とするビフィドバクテリウム菌の増殖促進および腸内有
害菌の増殖抑制剤。
(1) An agent for promoting the growth of Bifidobacterium and inhibiting the growth of harmful intestinal bacteria, the active ingredient being a syrup-like substance extracted from Yugao fruit.
JP63288928A 1988-11-17 1988-11-17 Bifidobacterium growth promotion and intestinal harmful bacteria growth inhibitor Expired - Lifetime JP2698128B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63288928A JP2698128B2 (en) 1988-11-17 1988-11-17 Bifidobacterium growth promotion and intestinal harmful bacteria growth inhibitor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP63288928A JP2698128B2 (en) 1988-11-17 1988-11-17 Bifidobacterium growth promotion and intestinal harmful bacteria growth inhibitor

Publications (2)

Publication Number Publication Date
JPH02135088A true JPH02135088A (en) 1990-05-23
JP2698128B2 JP2698128B2 (en) 1998-01-19

Family

ID=17736620

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Application Number Title Priority Date Filing Date
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Country Status (1)

Country Link
JP (1) JP2698128B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1972208A1 (en) 2007-03-16 2008-09-24 Kirin Holdings Kabushiki Kaisha Composition for improving intestinal microflora

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1972208A1 (en) 2007-03-16 2008-09-24 Kirin Holdings Kabushiki Kaisha Composition for improving intestinal microflora

Also Published As

Publication number Publication date
JP2698128B2 (en) 1998-01-19

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