JPH02242667A - Proliferation accelerator for bacterium of genus bifidobacterium - Google Patents

Abstract

PURPOSE:To readily enable production of bifidus factor having proliferation promoting effects by drying active ingredients of dishcloth gourd, cucumber, watermelon, pumpkin, yam, taro, 'KONJAK' (devil's-tongue), etc., and processing the dried active ingredients into a desired shape. CONSTITUTION:Active ingredients of dishcloth gourd, cucumber, sweet melon, pumpkin, yam, taro, 'KONJAK', Japanese radish, carrot, tomato, green pepper, okra, Welsh onion, Chinese cabbage, bean sprouts and tangerine orange, can be dried by freeze-drying, drying in the sun, hot-drying, etc., directly used or powdered and processed alone or two or more thereof can be mixed and processed into a desired shape, such as powder, granule or tablet. A fiber ingredient, such as sweet corn, an excipient, such as lactose, a sweetening ingredient, such as white sugar, a nutrient supply agent, such as milk powder, additives, such as aromatic agent, binder or lubricant, may be added to the above- mentioned proliferation accelerator. Thereby, the aforementioned accelerator is extremely useful in aspects of health promotion, since powerful proliferation activity of bacteria belonging to the genus Bifidobacterium is exhibited. The accelerator contributes to development of food industry, etc.

Description

[Detailed Description of the Invention] Industrial Application Field The present invention consists of loofah, gourd, watermelon, pumpkin, yam, taro, konnyaku, radish, carrot, tomato, biman, okra, green onion, Chinese cabbage, bean sprout, and mandarin orange. The present invention relates to a Bifidobacterium growth promoter containing one or more of the group as an active ingredient.

Conventional technology Bifidobacterium is non-pathogenic and exists mainly in the intestines of mammals, including humans, and has physiological effects such as suppressing intestinal putrefaction products and improving diarrhea and constipation. It is a useful bacterium with recognized significance.

In recent years, the effects of Bifidobacterium on promoting immunity, preventing bacterial replacement that occurs when antibiotics are administered continuously, and suppressing carcinogenic substances have been revealed, and its clinical application has become popular. (Microorganisms, Vol. 1, No. 4, p. 2, 1987, Medical Publishing Center). As described above, Bifidobacterium is deeply involved in the health and well-being of mammals including humans, and is attracting attention in the medical field.

There are two methods for growing Bifidobacterium in the intestines: a direct method of orally administering Bifidobacterium, and a method of administering a substance used by Phytobacterium coum (hereinafter referred to as Bifidobacterium). There is an indirect method of growing Phytobacterium. Direct methods have problems such as low stability of Bifidobacterium preparations and low bacterial colonization rates after administration, so indirect methods have recently been emphasized.

Conventionally, bifidus factors include N-7 cedilgluconamine, its derivatives, carrot extract (mainly containing pantethine), lactulose, raffinose, stachyose, malto-1 to liose (``Bifidobacterium'', 7
7 pages, 1979, Yakult Honsha Co., Ltd.), fructooligosaccharides (Chemistry and Biology, vol. 21, p. 291, 198
Galactooligosaccharide (Japanese Patent Publication No. 58-20266, Japanese Patent Publication No. 61-46479, Japanese Patent Application Laid-open No. 60-41449), isomalto-oligosaccharide (Japanese Society for Nutrition and Food Science, 1986) , cyclodextrin (Japanese Unexamined Patent Publication No. 138385/1983), konnyaku mannan (RIKEN Intestinal Flora Symposium ■, Intestinal Flora and Nutrition, p. 89, 1983, Gakkai Publishing Center), soy milk (Special Publication No. 138385/1983) @Publication,
JP-A-51-142566@publication, JP-A-55-853
90@publication), soy milk extract (JP-A-59-17906M
Publication), Extract of non-pathogenic Escherichia coli culture (Special Publication 1972-
13359M) are known.

In addition, in recent years, it has been discovered that dried Yugao fruit has a strong effect of promoting the growth of Bifidobacterium (Japanese Unexamined Patent Publication No. 63-291579). ni-pku, radish, carrot,
It is not known that tomatoes, green peppers, okra, green onions, Chinese cabbage, bean sprouts, and mandarin oranges have the effect of promoting the growth of Bifidobacterium bacteria.

R 朋1fi N'et-*Problems to be solved Most of the known bifidus factors mentioned above are also used by other intestinal bacteria in vivo, so they do not have sufficient growth effects on Bifidobacterium, and Their manufacturing methods are complicated;
It has drawbacks such as being expensive. Therefore, a bifidus factor that has a sufficient effect of promoting the growth of bifidobacteria and can be easily produced would be extremely useful industrially.

Means for Solving the Problems As a result of intensive research on the bifidus factor, the inventors found that loofah, gourd, watermelon, pumpkin, yam, taro, konjac, radish, carrot, 1 haemato, green pepper, okra, green onion, Chinese cabbage, and bean sprouts discovered that mandarin oranges have a Bifidobacterium growth-promoting effect similar to that of Yugao, and completed the present invention.

The loofah in the present invention refers to the fruit, vine, leaf, and petiole of the loofah, but preferably the loofah fruit.

Cucurbits as used in the present invention include cucurbit fruits, vines, sweets, and petioles, and preferably cucurbit fruits.

The term melon as used in the present invention refers to melon fruit, vines, leaves, and petioles, and preferably melon fruits, leaves, and petioles.

Watermelon as used in the present invention refers to watermelon fruits, vines, leaves, and petioles, preferably watermelon fruits.

Pumpkin seeds as used in the present invention include pumpkin fruits, vines, leaves, and petioles, but preferably pumpkin fruits.

The yam as used in the present invention is the root of the yam.

In the present invention, taro refers to the stems and leaves of taro.

Konnyaku in the present invention refers to the stems, leaves, and petioles of konnyaku.

The radish referred to in the present invention is the root of radish.

In the present invention, ginseng refers to ginseng roots, leaves, and petioles.
Preferably, carrot leaves and petioles are used.

Tomatoes, green peppers, and okra in the present invention are their respective fruits.

In the present invention, green onions, Chinese cabbage, and bean sprouts refer to the whole of each.

In the present invention, the term tangerine refers to a tangerine fruit, preferably the outer skin thereof.

The Phytobacterium growth promoter of the present invention can be prepared by drying the active ingredient by freeze-drying, sun-drying, heat-drying, etc., and use it as it is, powder it alone, or mix two or more kinds to form powders or granules. It can be processed into desired shapes such as drugs and tablets.

Among the Phytobacterium growth promoters of the present invention, fruits such as watermelons, melons, cucurbits, tomatoes, radish, vegetables such as Chinese cabbage, etc. are prepared by preparing fruit liquid or vegetable liquid by crushing, pressing filtration or centrifugation, and then Alternatively, after concentration, high-temperature instant sterilization or filtration sterilization can be performed, and each active ingredient can be used alone or in a mixture of two or more to form a liquid preparation. Furthermore, the squeezed lees after removing the liquid can be dried by the method described above to form powders, granules, and tablets.

Additives may be added to the Bifidobacterium growth promoter of the present invention, such as sweet corn, carrot powder, corn fiber, apple fiber, fiber components such as alginic acid, lactose,
Excipients such as starch, white sugar, maltose, sorbitol,
Sweet ingredients such as mannitol, nutritional supplements such as milk powder, meat extract, vitamins and calcium, aromatics,
Gum arabic powder, polyvinylpyrrolidone, hydroxypropyl cellulose striumium, calcium stearate, love wax,
Examples include lubricants such as talc, which may be appropriately selected and used depending on the shape of the Phytobacterium growth promoter and/or preference.

The Bifidobacterium growth promoter of the present invention can be used in confectionery, bread, noodles, tofu, hamburgers, fermentation holes, soft drinks,
May be added to other foods.

Among the Bifidobacterium growth promoters of the present invention, powders and granules may be added to miso soup base, soup base, furikake, etc., and can also be used as feed additives for livestock such as cows and pigs, and for dogs. It can also be used as a feed additive for cats, etc.

Example 1 After crushing 987 g of loofah fruits, the juice was made into juice using a blender, and the juice was freeze-dried. Next, the freeze-dried product was ground with a coffee mill to form powder 67Q with a water content of 7.7%.
I got it.

Example 2 Using 919 g of cucurbit fruit as a raw material, 71 g of powder with a moisture content of 5.6% was prepared in the same manner as in Example 1.

Example 3 Using 854 g of melon fruit as a raw material, 116 q of powder with a water content of 6.9% was prepared in the same manner as in Example 1.

Example 4 Using 459 g of melon leaves and petioles as raw materials, 62 g of powder with a moisture content of 4.6% was prepared in the same manner as in Example 1.

Example 5 Using watermelon fruit 2059C7 as a raw material, 148 g of powder with a water content of 6.7% was obtained in the same manner as in Example].

Example 6 Using 1106 g of pumpkin fruit as a raw material, the same procedure as in Example 1 was carried out to obtain 288 g of powder with a moisture content of 5.0%.

Example 7 Using yam root 203q as a raw material, the same procedure as in Example 1 was carried out to obtain powder 54Q with a water content of 3.3%.

Example 8 Using 695q of taro stems as a raw material, 19 pieces of powder 4RJ with a moisture content of 7.8% were prepared in the same manner as in Example 1.

Example 9 Using konjac leaves and petiole 500C] as raw materials, the same procedure as in Example 1 was carried out to prepare powder 37C with a moisture content of 4.6% (q
is.

Example 10 Using radish root 370 CI as a raw material, the same procedure as in Example 1 was carried out to obtain powder 250 with a water content of 7.9%.

Example 11 Using 867 g of petioles of carrot cake as a raw material, the same procedure as in Example 1 was carried out to obtain 102 g of powder with a water content of 5.2%.

Example 12 Using 973q of tomato fruit as a raw material, the same procedure as in Example 1 was carried out to obtain 41 g of powder with a moisture content of 6.6%.

Example 13 Using 604 g of green pepper fruit as a raw material, 1 q of 43 g of powder with a moisture content of 5.5% was prepared in the same manner as in Example Go.

Example 14 Using 420 q of okra fruit as a raw material, the same procedure as in Example 1 was carried out to obtain 38 g of powder with a water content of 7.4%.

Example 15 Using 466 g of green onions as a raw material, 31 g of powder with a water content of 6.7% was prepared in the same manner as in Example 1.

Example 15 Using 696 g of Chinese cabbage as a raw material, the same procedure as in Example 1 was carried out to prepare powder 23Q with a moisture content of 5.5% (q).

Example 17 Using 1021 g of bean sprouts as a raw material, 1 q of 50 CJ of powder with a water content of 5.3% was prepared in the same manner as in Example 1.

Example 18 Using 230 g of mandarin orange peel as a raw material, the same procedure as in Example 1 was carried out to obtain 54 g of powder with a water content of 5.1%.

Test Example The Bifidobacterium growth-promoting activity of samples produced in the same manner as in Examples 1 to 18 was measured using the amount of acid produced as an indicator using the method described below.

1゜ Bacterial strain used Japanese freeze-drying research fracture Bifidobacterium rondum (Biridobacterium + n lonqum)
Bacteria powder, Bifidus 3 billion.

2. Culture method: Place 10 samples in a small test tube (16,5X 1651nffl).
011! O was weighed out and mixed with 4 ml of distilled water and 2 times the strength of Tomarei modified medium [Reports of
Re5search Laboratory
chnical Re5earch In5titut
e, Snow BrandMilk Product
s Co, Ltd, 72.1 (197?)] 5
After adding 5 m and attaching an aluminum cap, sterilize in an autoclave at 121°C for 10 minutes.

Next, 1 ml of a 1% ascorbic acid solution (pH 6.5) sterilized by filtration using a 0.3 μm membrane filter is added. Three bottles of this medium were prepared for each sample, and 0.1 ml of Bifidobacterium solution (diluted to 3000 Ce1ls/ml) was added to two bottles, and 0.1 ml of sterile distilled water was added to the remaining bottle as a blank test. , 37 by gas back method
The cells were cultured anaerobically at ℃ for 24 to 48 hours, and the amount of acid produced was measured. Control 1 is a non-additive medium, control 2 is Yugao fruit (
A culture medium to which dried gourd (Japanese gourd) powder was prepared was prepared and operated in the same manner.

3. Measurement of acid production amount 5 ml of the culture solution was weighed into an Erlenmeyer flask, and 0.5 ml was measured using phenolphthalene as an indicator. Titrated with 1N-Na011,
After subtracting the blank test value, the average value of the two samples was determined, and the alkali titer per 10 ml of culture solution was taken as the acid production amount. The results are shown in Table 1.

(Margins below) Table 1 Table 1 (Continued) As shown in Table 1, all of the bifidobacterium growth promoters of the present invention had the same strong bifidobacterium growth promoting activity as Yugao fruit (dried gourd) powder. .

The Bifidobacterium growth promoter of the present invention exhibits a strong Phytobacterium growth activity, and is therefore extremely useful in terms of health promotion.

In addition, the Bifidobacterium multiplication promoter of the present invention can be used in the food industry and This will greatly contribute to the development of agriculture.

Claims (1)

[Claims] (1) Loofah, gourd, watermelon, pumpkin, yam, taro,
Konjac, radish, carrot, tomato, green pepper, okra,
A Bifidobacterium growth promoter containing one or more of the group consisting of green onions, Chinese cabbage, bean sprouts, and mandarin oranges as an active ingredient.