KR20210114325A - Prebiotics comprising Pear Extract, Synbiotics Compositions comprising the Same and Uses thereof - Google Patents
Prebiotics comprising Pear Extract, Synbiotics Compositions comprising the Same and Uses thereof Download PDFInfo
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- KR20210114325A KR20210114325A KR1020200168612A KR20200168612A KR20210114325A KR 20210114325 A KR20210114325 A KR 20210114325A KR 1020200168612 A KR1020200168612 A KR 1020200168612A KR 20200168612 A KR20200168612 A KR 20200168612A KR 20210114325 A KR20210114325 A KR 20210114325A
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- pear
- present
- prebiotic
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Images
Classifications
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- A—HUMAN NECESSITIES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
Description
본 발명은 배 추출물을 유효성분으로 포함하는 프리바이오틱 조성물에 관한 것이다.The present invention relates to a prebiotic composition comprising a pear extract as an active ingredient.
인간을 포함하는 포유동물의 장 내에는 다양한 미생물이 서식하고 있으며 약 300 종류, 약 100조의 세균이 존재한다고 알려져 있다. 장 내에는 인체에 대한 유익균과 유해균이 일정한 비율로 균형을 이루고 있으며, 특히 유익균은 소화를 돕거나 유익한 물질을 생산하는 등의 건강을 유지하는 역할을 하기도 하지만, 유익균의 비율이 낮아지면 각종 질병의 원인이 되고 있다. It is known that various microorganisms inhabit the intestines of mammals including humans, and there are about 300 types of bacteria and about 100 trillion bacteria. In the intestine, beneficial bacteria and harmful bacteria for the human body are balanced in a certain ratio. being the cause
프로바이오틱스(probiotics)는 유익균인 유산균 제제 또는 유산균의 성분으로 제조되며, 유산균으로는 비피도박테리움속(Bifidobacterium genus)과 락토바실러스속(Lactobacillus genus) 등이 대표적이다. 프로바이오틱스 기능은 장내에서 병원균 증식을 억제하고, 장상피세포나 면역세포로 하여금 면역 능력을 조절하는 물질을 생성시키며, 병원균과 경합하여 병원균의 증식을 억제하고, 이차적으로는 항생제 치료나 외부 환경 변화에 대해 장내 세균총의 항상성을 유지하게 하는 것으로 알려져 있다.Probiotics are produced from lactic acid bacteria preparations or components of lactic acid bacteria, which are beneficial bacteria, and examples of lactic acid bacteria include Bifidobacterium genus and Lactobacillus genus. The probiotic function inhibits the growth of pathogens in the intestine, produces substances that regulate the immune ability of intestinal epithelial cells or immune cells, inhibits the growth of pathogens by competing with pathogens, and secondaryly prevents antibiotic treatment or changes in the external environment. It is known to maintain the homeostasis of the intestinal flora.
프로바이오틱스의 효과는 비피도박테리움속이나 락토바실러스속의 장내 유용 미생물 수를 증가시킴으로써 유해 미생물 수가 감소되면 장내 항상성 유지에 유용할 것이라는 사고를 근거로 다양한 실험을 통해 입증되어 왔다. 그러나 모든 프로바이오틱스가 항상 유용한 것은 아니며, 같은 속의 장내 세균이라 하더라도 종에 따라 임상 효과가 있기도 하고, 또는 아예 없는 경우도 있으며, 일부에서는 오히려 임상 경과를 악화시키기는 경우도 있는 것으로 알려져 있다.The effect of probiotics has been proven through various experiments based on the idea that if the number of harmful microorganisms decreases by increasing the number of useful microorganisms in the intestine of the genus Bifidobacterium or Lactobacillus, it will be useful in maintaining intestinal homeostasis. However, not all probiotics are always useful, and it is known that even intestinal bacteria of the same genus may have clinical effects depending on the species, or may have no clinical effect, and in some cases worsen the clinical course.
한편, 프로바이오틱스의 기능을 증진하기 위해 유익균이 장내 잘 정착시키기고 성장에 도움을 주기 위한 유산균의 먹이인 프리바이오틱스에 대한 연구가 보고되고 있다. 즉, 프리바이오틱스는 유익균이 장에서 정착해 생존하기 위한 일종의 먹이에 해당하는 것으로, 유익균에 먹이만 충분히 공급되는 경우 1마리가 하루만에 200억 마리로 증식한다. 최근에는 프로바이오틱스와 프리바이오틱스를 혼합한 제품인 신바이오틱스가 등장하였다.On the other hand, to enhance the function of probiotics, studies on prebiotics, which are food for lactic acid bacteria, are being reported to help beneficial bacteria to settle well in the intestine and to help them grow. In other words, prebiotics are a kind of food for beneficial bacteria to settle and survive in the intestine. Recently, synbiotics, a product that mixes probiotics and prebiotics, have emerged.
본 발명자들은 이와 같은 점을 감안하여 배 추출물이 유산균의 성장을 촉진하는 것을 확인함으로써, 본 발명을 완성하였다.The present inventors have completed the present invention by confirming that the pear extract promotes the growth of lactic acid bacteria in view of this point.
본 발명은 배 추출물이 장내 유익균용 배지에서 락토바실러스 애시도필러스(Lactobacillus acidophilus) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus) 균주의 성장을 촉진하는 것을 확인함으로써, 배 추출물을 유효성분으로 포함하는 프리바이오틱 조성물, 상기 프리바이오틱 조성물을 포함하는 신바이오틱 조성물, 및 상기 항의 신바이오틱 조성물을 포함하는 기능성 식품 조성물을 제공하는 것이다. The present invention confirms that the pear extract promotes the growth of Lactobacillus acidophilus and Streptococcus thermophilus strains in a medium for beneficial intestinal bacteria, thereby confirming that a free containing pear extract as an active ingredient It is to provide a biotic composition, a synbiotic composition comprising the prebiotic composition, and a functional food composition comprising the synbiotic composition of the above item.
본 발명은 배 추출물을 유효성분으로 포함하는 프리바이오틱 조성물을 제공한다. The present invention provides a prebiotic composition comprising a pear extract as an active ingredient.
또한, 본 발명은 상기 프리바이오틱 조성물을 포함하는 신바이오틱 조성물을 제공한다.In addition, the present invention provides a synbiotic composition comprising the prebiotic composition.
또한, 본 발명은 상기 신바이오틱 조성물을 포함하는 건강기능성 원료 조성물을 제공한다.In addition, the present invention provides a health functional raw material composition comprising the synbiotic composition.
또한, 본 발명은 상기 신바이오틱 조성물을 포함하는 사료 조성물을 제공한다.In addition, the present invention provides a feed composition comprising the synbiotic composition.
본 발명에 따르면, 배 추출물이 장내 유익균용 배지에서 락토바실러스 애시도필러스(Lactobacillus acidophilus) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus) 균주의 성장을 촉진하는 것을 확인함으로써, 배 추출물을 프리바이오틱 조성물로 제공될 수 있다.According to the present invention, by confirming that the pear extract promotes the growth of Lactobacillus acidophilus and Streptococcus thermophilus strains in the medium for beneficial intestinal bacteria, the pear extract is used as a prebiotic composition can be provided as
도 1은 본 발명에 따라 프리바오이틱 조성물을 제조하는 방법을 나타내는 그림이다.
도 2는 본 발명에 따른 프리바오이틱 조성물을 투여하였을 때의 프로바이오틱 유산균인 락토바실러스 애시도필러스(Lactobacillus acidophilus) 균주의 시간에 따른 성장 곡선을 나타내는 그래프이다.
도 3은 본 발명에 따른 프리바오이틱 조성물을 투여하였을 때의 프로바이오틱 유산균인 스트렙토코커스 써모필러스(Streptococcus thermophilus) 균주의 시간에 따른 성장 곡선을 나타내는 그래프이다.
도 4는 본 발명에 따른 프리바오이틱 조성물을 투여하였을 때의 프로바이오틱 유산균인 락토바실러스 애시도필러스(Lactobacillus acidophilus) 균주의 시간에 따른 성장 곡선을 프락토올리고당 및 갈락토올리고당과 비교한 그래프이다.
도 5는 배 추출물을 포함하는 프리바이오틱 조성물의 기능을 나타내는 그림이다.1 is a diagram showing a method for preparing a prebiotic composition according to the present invention.
Figure 2 is a graph showing the growth curve over time of the probiotic lactic acid bacteria, Lactobacillus acidophilus ( Lactobacillus acidophilus ) strain when the prebiotic composition according to the present invention is administered.
3 is a graph showing the growth curve over time of a Streptococcus thermophilus strain, a probiotic lactic acid bacterium, when the prebiotic composition according to the present invention is administered.
Figure 4 is a graph comparing the growth curve with time of the Lactobacillus acidophilus strain, a probiotic lactic acid bacterium, with fructooligosaccharide and galactooligosaccharide when the prebiotic composition according to the present invention is administered. am.
5 is a diagram showing the function of a prebiotic composition containing a pear extract.
본 명세서에서 사용되는 용어는 본 발명에서의 기능을 고려하면서 가능한 현재 널리 사용되는 일반적인 용어들을 선택하였으나, 이는 당 분야에 종사하는 기술자의 의도 또는 판례, 새로운 기술의 출현 등에 따라 달라질 수 있다. 또한, 특정한 경우는 출원인이 임의로 선정한 용어도 있으며, 이 경우 해당되는 발명의 설명 부분에서 상세히 그 의미를 기재할 것이다. 따라서 본 발명에서 사용되는 용어는 단순한 용어의 명칭이 아닌, 그 용어가 가지는 의미와 본 발명의 전반에 걸친 내용을 토대로 정의되어야 한다.The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.
다르게 정의되지 않는 한, 기술적이거나 과학적인 용어를 포함해서 여기서 사용되는 모든 용어들은 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 가지고 있다. 일반적으로 사용되는 사전에 정의되어 있는 것과 같은 용어들은 관련 기술의 문맥상 가지는 의미와 일치하는 의미를 가지는 것으로 해석되어야 하며, 본 출원에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.Unless defined otherwise, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not
수치 범위는 상기 범위에 정의된 수치를 포함한다. 본 명세서에 걸쳐 주어진 모든 최대의 수치 제한은 낮은 수치 제한이 명확히 쓰여져 있는 것처럼 모든 더 낮은 수치 제한을 포함한다. 본 명세서에 걸쳐 주어진 모든 최소의 수치 제한은 더 높은 수치 제한이 명확히 쓰여져 있는 것처럼 모든 더 높은 수치 제한을 포함한다. 본 명세서에 걸쳐 주어진 모든 수치 제한은 더 좁은 수치 제한이 명확히 쓰여져 있는 것처럼, 더 넓은 수치 범위 내의 더 좋은 모든 수치 범위를 포함할 것이다.Numerical ranges are inclusive of the values defined in that range. Every maximum numerical limitation given throughout this specification includes all lower numerical limitations as if the lower numerical limitation were expressly written. Every minimum numerical limitation given throughout this specification includes all higher numerical limitations as if the higher numerical limitation were expressly written. Any numerical limitation given throughout this specification shall include all numerical ranges within the broader numerical range, as if the narrower numerical limitation were expressly written.
이하, 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명은 배 추출물을 유효성분으로 포함하는 프리바이오틱 조성물을 제공한다.The present invention provides a prebiotic composition comprising a pear extract as an active ingredient.
상기 배는 배 나무의 열매로, 당분 10∼14％, 과육 100g에 칼륨 140∼170mg, 비타민C 3∼6mg이 함유되어 있어 껍질은 누르께한 빛을 띠고 과육은 희고 단단하면서 단맛이 나는 과일이다.The pear is a fruit of the pear tree, and contains 10-14% sugar, 140-170 mg of potassium, and 3-6 mg of vitamin C in 100 g of the pulp.
상기 배 추출물은 배 나무(Pyrus pyrifolia cv. Shingo 또는 Pyrus pyrifolia cv. Hwangkeumbae)의 껍질을 포함하는 열매 또는 열매 껍질을 추출 용매로 추출한 것일 수 있다. 상기 추출 용매는 20% 내지 90%의 에탄올일 수 있다.The pear extract is a pear tree ( Pyrus pyrifolia cv. Shingo or Pyrus pyrifolia cv. Hwangkeumbae) may be a fruit containing the peel or a fruit peel extracted with an extraction solvent. The extraction solvent may be 20% to 90% ethanol.
상기 프리바이오틱스는 미생물의 영양원이 되며, 본 발명에 따른 상기 프리바이오틱 조성물은 장내 유익균의 성장 및 증식 촉진할 수 있다.The prebiotics serve as a nutrient source for microorganisms, and the prebiotic composition according to the present invention can promote the growth and proliferation of beneficial intestinal bacteria.
상기 장내 유익균은 락토바실러스 속, 스트렙토코커스 속 또는 비피더스 속 균주일 수 있으며, 구체적으로, 락토바실러스 애시도필러스(Lactobacillus acidophilus) 또는 스트렙토코커스 써모필러스(Streptococcus thermophilus)일 수 있으나, 이에 제한되는 것은 아니다.The intestinal beneficial bacteria may be Lactobacillus genus, Streptococcus genus or Bifidos genus strains, specifically, Lactobacillus acidophilus or Streptococcus thermophilus ), but may be limited thereto. no.
또한, 본 발명은 상기 프리바이오틱 조성물을 포함하는 신바이오틱 조성물을 제공한다.In addition, the present invention provides a synbiotic composition comprising the prebiotic composition.
상기 신바이오틱 조성물은 경구 투여에 용이하도록 당업계의 통상적인 방법으로 캡슐, 비즈(beads), 음용할 수 있는 용액, 현탁액, 파우더, 과립, 정제, 발포제 등으로 제조될 수 있다.The synbiotic composition may be prepared into capsules, beads, edible solutions, suspensions, powders, granules, tablets, foaming agents, and the like by a conventional method in the art for easy oral administration.
또한, 본 발명은 상기 신바이오틱 조성물을 포함하는 기능성 식품 조성물을 제공한다.In addition, the present invention provides a functional food composition comprising the synbiotic composition.
본 발명은 통상적으로 이용되는 식품으로써 일반적으로 사용될 수 있다.The present invention can be generally used as a commonly used food product.
본 발명의 식품 조성물은 건강기능식품으로서 사용될 수 있다. 상기 "건강기능 식품"이라 함은 건강기능 식품에 관한 법률에 따른 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 및 가공한 식품을 의미하며, "기능성"이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻을 목적으로 섭취하는 것을 의미한다.The food composition of the present invention can be used as a health functional food. The term "health functional food" means a food manufactured and processed using raw materials or ingredients useful for the human body in accordance with the Health Functional Food Act, and "functionality" refers to the structure and function of the human body. It refers to ingestion for the purpose of obtaining useful effects for health purposes such as regulating nutrients or physiological effects.
본 발명의 식품 조성물은 통상의 식품 첨가물을 포함할 수 있으며, 상기 "식품 첨가물"로써 적합 여부는 다른 규정이 없는 한, 식품의약품안전처에 승인된 식품 첨가물 공전의 총칙 및 일반시험법 등에 따라 해당 품목에 관한 규격 및 기준에 의하여 판정한다.The food composition of the present invention may contain conventional food additives, and whether or not it is suitable as the "food additive" is determined according to the general rules and general test methods of food additives approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to the standards and standards related to the item.
상기 "식품 첨가물 공전"에 수재된 품목으로는 예를 들어, 케톤류, 글리신, 구연산칼륨, 니코틴산, 계피산 등의 화학적 합성물, 감색소, 감초추출물, 결정셀룰로오스, 고량색소, 구아검 등의 천연첨가물, L-글루타민산나트륨 제제, 면류첨가알칼리제, 보존료제제, 타르색소제제 등의 혼합제제류들을 들 수 있다.The items listed in the “Food Additives Code” include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; Mixed preparations such as sodium L-glutamate preparation, noodle-added alkali agent, preservative agent, and tar color agent can be mentioned.
본 발명의 식품 조성물은 정제, 캡슐, 분말, 과립, 액상, 환 등의 형태로 제조 및 가공할 수 있다.The food composition of the present invention may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like.
예를 들어, 캡슐 형태의 건강기능 식품 중 경질캡슐제는 통상의 경질캡슐에 본 발명에 따른 조성물을 부형제 등의 첨가제와 혼합 및 충진하여 제조할 수 있으며, 연질캡슐제는 본 발명에 따른 조성물을 부형제 등의 첨가제와 혼합하고 젤라틴 등 캡슐기제에 충진하여 제조할 수 있다. 상기 연질캡슐제는 필요에 따라 글리세린 또는 소르비톨 등의 가소제, 착색제, 보존제 등을 함유할 수 있다.For example, among the health functional foods in the form of capsules, hard capsules can be prepared by mixing and filling a conventional hard capsule with the composition according to the present invention with additives such as excipients, and the soft capsule contains the composition according to the present invention. It can be prepared by mixing with additives such as excipients and filling in capsule bases such as gelatin. The soft capsule formulation may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary.
상기 부형제, 결합제, 붕해제, 활택제, 교미제, 착향제 등에 대한 용어 정의는 당업계에 공지된 문헌에 기재된 것으로 그 기능 등이 동일 내지 유사한 것들을 포함한다. 상기 식품의 종류에는 특별한 제한이 없으며, 통상적인 의미에서의 건강기능식품을 모두 포함한다.The term definitions for the excipients, binders, disintegrants, lubricants, flavoring agents, and the like are described in documents known in the art and include those having the same or similar functions. There is no particular limitation on the type of food, and includes all health functional foods in the ordinary sense.
또한, 본 발명은 상기 신바이오틱 조성물을 포함하는 사료 조성물을 제공한다. In addition, the present invention provides a feed composition comprising the synbiotic composition.
본 발명은 사료에 동물의 면역기능 항진 및 항생제 투여로 인한 균교대증 등 부작용을 낮추기 위하여 프로바이오틱스 뿐만 아니라 유산균 성장 촉진을 위한 프리바이오틱스가 함께 혼합된 신바이오틱스를 포함하는 사료 조성물을 제공한다.The present invention provides a feed composition comprising synbiotics mixed with probiotics as well as prebiotics for promoting the growth of lactic acid bacteria in order to improve the immune function of animals and reduce side effects such as mycosis caused by the administration of antibiotics to the feed.
상기 사료는 가축이 섭취하고, 소화시키기 위한 또는 이에 적당한 임의의 천연 또는 인공 규정식, 한끼식 등 또는 상기 한끼식의 성분을 의미한다. 상기 사료는 사료 첨가제 또는 보조사료를 포함할 수 있다.The feed means any natural or artificial diet, meal meal, etc., or a component of the meal meal, intended for or suitable for ingestion and digestion by livestock. The feed may include a feed additive or a supplementary feed.
상기 사료의 종류는 특별히 제한되지 않으며, 당해 기술 분야에서 통상적으로 사용되는 사료를 사용할 수 있다. 상기 사료의 비제한적인 예로는, 곡물류, 근과류, 식품 가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류 또는 곡물 부산물류 등과 같은 식물성 사료: 단백질류, 무기물류, 유지류, 광물성류, 유지류, 단세포 단백질류, 동물성 플랑크톤류 또는 음식물 등과 같은 동물성 사료를 들 수 있다. 이들은 단독으로 사용되거나 2종 이상을 혼합하여 사용될 수 있다.The type of feed is not particularly limited, and feed commonly used in the art may be used. Non-limiting examples of the feed include plant feeds such as grains, root fruits, food processing by-products, algae, fibers, pharmaceutical by-products, oils and fats, starches, gourd or grain by-products: proteins, inorganics, and animal feeds such as oils and fats, minerals, oils and fats, unicellular proteins, zooplankton, or food. These may be used alone or may be used in combination of two or more.
이하, 본 발명의 이해를 돕기 위하여 실시예 및 실험예를 들어 상세하게 설명하기로 한다. 다만 하기의 실시예 및 실험예는 본 발명의 내용을 예시하는 것일 뿐 본 발명의 범위가 하기 실시예 및 실험예에 한정되는 것은 아니다. 본 발명의 실시예 및 실험예는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다.Hereinafter, examples and experimental examples will be described in detail to help the understanding of the present invention. However, the following Examples and Experimental Examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited to the following Examples and Experimental Examples. Examples and experimental examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.
실시예 1: 프리바이오틱스 조성물의 제조 1 (알코올 침지추출법)Example 1: Preparation of prebiotic composition 1 (alcohol immersion extraction method)
본 발명의 프리바이오틱스 조성물은 도 1에 나타난 바와 같이 한국산 황금배(Pyrus pyrifolia cv. Whanggeumbae)을 추출하여 사용하였다. 껍질을 포함하는 상태의 황금배를 정제수로 3회 세척 후, 6 내지 8 조각으로 절단하고 속심을 제거한 후, 녹즙기(Hurom Celeb, ㈜휴롬, 경남 김해시)로 착즙하여 배껍질과 배즙을 분리한 후 배껍질, 배즙, 그리고 배껍질과 배즙의 혼합액을 이용하여 각각 다음과 같이 액상 또는 과립형으로 제조하였다. As shown in FIG. 1, the prebiotic composition of the present invention was used by extracting Korean golden pears (Pyrus pyrifolia cv. Whanggeumbae). After washing golden pears in a state containing the
(1) 배껍질을 30% 에탄올에 침지하여 실온에서 3일간 추출하고, 55 μm bag filter 로 여과하여 여과액을 수득하였다. 수득된 여과액은 14.1 brix로 80℃, 1200 rpm 조건으로 농축하였다(액상제품).(1) The pear skin was immersed in 30% ethanol, extracted for 3 days at room temperature, and filtered through a 55 μm bag filter to obtain a filtrate. The obtained filtrate was concentrated with 14.1 brix at 80°C and 1200 rpm (liquid product).
과립으로 제조하기 위해서 농축액 고형분과 덱스트린을 혼합하되, 총 혼합물 대비 덱스트린이 70% 를 넘지 않도록 첨가하였다. 혼합물을 180℃ - 200℃ 에서 30초간 분무 건조 후, 최종 95℃에서 과립시료를 얻었다 (과립형 제품).In order to prepare granules, the concentrate solids and dextrin were mixed, but dextrin was added so as not to exceed 70% of the total mixture. After spray-drying the mixture at 180°C - 200°C for 30 seconds, a final granular sample was obtained at 95°C (granular product).
실시예 1의 방법에 대한 모식도는 도 1에 나타나있다.A schematic diagram of the method of Example 1 is shown in FIG. 1 .
실시예 2: 프리바이오틱스 조성물의 제조 2 (대량생산을 위한 알코올 침지추출법)Example 2: Preparation of prebiotic composition 2 (alcohol immersion extraction method for mass production)
본 발명의 프리바이오틱스 조성물의 제조방법은 실시예 1의 (1) 방법을 수정하여 대량생산을 위하고 또한 소량으로 섭취를 용이하게 하기 위하여 농축이 강화된 방법을 제공한다.The preparation method of the prebiotic composition of the present invention provides a method with enhanced concentration in order to facilitate mass production and ingestion in a small amount by modifying method (1) of Example 1.
본 발명의 프리바이오틱스 조성물은 한국산 신고배(Pyrus pyrifolia cv. Shingo)을 추출하여 사용하였다. 껍질을 포함하는 상태의 신고배를 정제수로 3회 세척 후, 6 내지 8 조각으로 절단하고 속심을 제거한 후, 착즙기(과채류 스크류, ㈜동방식품기계, 경남 양산시)로 착즙하여 배껍질과 배즙을 분리하였다.The prebiotic composition of the present invention was used by extracting Korean pear (Pyrus pyrifolia cv. Shingo). After washing the Shingo pear in a state containing the
배껍질을 30% 에탄올에 침지하여 실온에서 3일간 추출하고, 55 μm bag filter 로 여과하여 여과액을 수득하였다. 수득된 여과액은 9 brix로 80℃, 1200 rpm 조건하에 59 brix로 농축하였다.The pear skin was immersed in 30% ethanol, extracted for 3 days at room temperature, and filtered through a 55 μm bag filter to obtain a filtrate. The obtained filtrate was concentrated to 59 brix at 80° C. and 1200 rpm at 9 brix.
비교예 1: 프리바이오틱스 조성물의 제조 3 (초고압 추출)Comparative Example 1: Preparation of prebiotic composition 3 (ultra-high pressure extraction)
껍질을 포함하는 상태의 황금배를 정제수로 3회 세척 후, 6 내지 8 조각으로 절단한 후, 진공포장용 비닐백에 넣어 진공포장기(SaengQ, (주) 생큐)로 진공포장한후 초고압기로 400 MPa (4000 bar) 또는 500 MPa (5000 bar)의 압력으로 분쇄한 후, 녹즙기(Hurom Celeb, (주)휴롬, 경남 김해시)로 배즙과 배껍질로 분리하였다.After washing the golden pear in the state containing the
분쇄된 배껍질을 30% 에탄올에 침지하여 실온에서 3일간 추출하고, 55 μm bag filter 로 여과하여 여과액을 수득하였다. rotary evaporator로 농축 후 60% 에탄올에 용해하였다.The pulverized pear skin was immersed in 30% ethanol, extracted for 3 days at room temperature, and filtered through a 55 μm bag filter to obtain a filtrate. After concentration with a rotary evaporator, it was dissolved in 60% ethanol.
비교예 2: 프리바이오틱스 조성물의 제조 4 (초임계 추출)Comparative Example 2: Preparation of prebiotic composition 4 (supercritical extraction)
껍질을 포함하는 상태의 황금배를 정제수로 3회 세척 후, 6 내지 8 조각으로 절단한 후, 동결건조 하였다. 건조 고형물을 350 bar, 50℃ 조건에서 CO2로 60 분 동안 추출한 후, 5% 에탄올을 첨가하여 120 분 동안 추출하였다. rotary evaporator로 농축 후 60% 에탄올에 용해하였다.After washing the golden pear in a state containing the
실험예 1: 배추출물이 프로바이오틱 유산균 성장에 미치는 영향Experimental Example 1: Effect of pear extract on growth of probiotic lactic acid bacteria
실시예의 배 추출물이 프리바이오틱스로서 장내 유익균의 증식에 미치는 영향을 평가하기 위해, 프로바이오틱 유산균인 락토바실러스 애시도필러스(Lactobacillus acidophilus) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)의 성장에 미치는 영향을 확인하였다.In order to evaluate the effect of the pear extract of Examples on the proliferation of beneficial intestinal bacteria as a prebiotic, probiotic lactic acid bacteria Lactobacillus acidophilus ( Lactobacillus acidophilus ) and Streptococcus thermophilus ( Streptococcus thermophilus ) On the growth of The effect was confirmed.
락토바실러스 애시도필러스(Lactobacillus acidophilus) 또는 스트렙토코커스 써모필러스(Streptococcus thermophilus) 균주를 MRS 배지에 배양하되, 실험군 배지에는 배 추출물을 1 mg/mL 첨가하였다. 다른 대조군에는 60% 에탄올만 MRS 배지에 첨가하였다. Lactobacillus acidophilus ( Lactobacillus acidophilus ) or Streptococcus thermophilus strains were cultured in MRS medium, and 1 mg/mL of pear extract was added to the experimental group medium. In the other control group, only 60% ethanol was added to the MRS medium.
각각의 배지에 균주를 600nm에서 흡광도(optical Density)가 0.2가 되도록 접종하고, 락토바실러스는 37℃에서, 스트렙토코커스 써모필러스는 42℃에서 정치 배양하였다. 5 시간 간격으로 OD600을 측정하여 각각의 균주의 성장 곡선으로 그래프화 하였다(도 2 및 3).In each medium, the strain was inoculated to have an absorbance (optical Density) of 0.2 at 600 nm, Lactobacillus at 37 ° C, and Streptococcus thermophilus at 42 ° C. OD600 was measured at 5-hour intervals and graphed as a growth curve of each strain ( FIGS. 2 and 3 ).
도 2 및 3에 나타난 바와 같이, 실시예 1의 배 추출물을 첨가한 배지에서 락토바실러스 애시도필러스(Lactobacillus acidophilus) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus) 균주 모두 성장곡선이 대조군들에 비해 확연하게 성장한 것으로 나타났다.As shown in Figures 2 and 3, in the medium to which the pear extract of Example 1 was added, both Lactobacillus acidophilus and Streptococcus thermophilus strains showed significant growth curves compared to the control groups. appeared to have grown significantly.
상기 결과는 실시예 1의 배 추출물이 장내 유익균인 프로바이오틱 균주의 성장을 촉진하는 효과가 있다는 것을 입증하였으며, 이러한 결과는 본 발명에 따른 배 추출물은 프리바이오틱스로 사용될 수 있다는 것을 시사한다.The above results demonstrated that the pear extract of Example 1 has an effect of promoting the growth of probiotic strains, which are beneficial intestinal bacteria, and these results suggest that the pear extract according to the present invention can be used as a prebiotic.
실험예 2: 배추출물과 프리바이오틱스 올리고당이 프로바이오틱 유산균 성장에 미치는 영향의 비교Experimental Example 2: Comparison of the effect of pear extract and prebiotic oligosaccharides on growth of probiotic lactic acid bacteria
실시예의 배 추출물이 프리바이오틱스로서 장내 유익균의 증식에 미치는 영향을 대표적인 프리바이오틱스인 프락토올리고당 및 갈락토올리고당과 비교평가하기 위해, 프로바이오틱 유산균인 락토바실러스 애시도필러스(Lactobacillus acidophilus)의 성장에 미치는 영향을 확인하였다.In order to compare and evaluate the effect of the pear extract of Example on the proliferation of beneficial intestinal bacteria as a prebiotic with fructooligosaccharide and galactooligosaccharide, which are representative prebiotics, the probiotic lactic acid bacteria Lactobacillus acidophilus ( Lactobacillus acidophilus ) was confirmed to have an effect on the growth of
락토바실러스 애시도필러스(Lactobacillus acidophilus) 균주를 MRS 배지에 배양하되, 실험군 배지에는 배 추출물, 프락토올리고당 또는 갈락토올리고당을 3 mg/mL 첨가하였다. Lactobacillus acidophilus ( Lactobacillus acidophilus ) The strain was cultured in MRS medium, and 3 mg/mL of pear extract, fructooligosaccharide or galactooligosaccharide was added to the experimental group medium.
각각의 배지에 균주를 600nm에서 흡광도(optical Density)가 0.2가 되도록 접종하고, 락토바실러스를 37℃에서 정치 배양하였다. 5 시간 간격으로 OD600을 측정하여 각각의 균주의 성장 곡선으로 그래프화 하였다(도 4).In each medium, the strain was inoculated to have an absorbance (optical Density) of 0.2 at 600 nm, and Lactobacillus was stationary cultured at 37°C. OD600 was measured at 5-hour intervals and graphed as a growth curve of each strain (FIG. 4).
도 4에 나타난 바와 같이, 실시예 2의 배 추출물을 첨가한 배지에서 락토바실러스 애시도필러스(Lactobacillus acidophilus) 균주의 성장곡선이 프락토올리고당 또는 갈락토올리고당을 첨가한 배지에서의 성장곡선과 거의 동등하게 성장한 것으로 나타났다. As shown in Figure 4, the growth curve of the Lactobacillus acidophilus strain in the medium to which the pear extract of Example 2 was added was almost the same as the growth curve in the medium to which fructooligosaccharide or galactooligosaccharide was added. appeared to grow equally.
상기 결과는 실시예 2의 배 추출물이 장내 유익균인 프로바이오틱 균주의 성장을 촉진하는 프리바이오틱스로써 식약처의 장건강 기능면에서 허가를 득한 제품인 프락토올리고당 및 갈락토올리고당과 거의 동등하다는 것을 입증하였으며, 배추출물은 위 당류와 달리 다음에 서술한 arbutin, chlorogenic acid 등 polyphenol 성 항산화물질을 포함한 다양한 항알러지성분, 숙취해독 성분 등이 들어있어서(도5) 본 발명에 따른 배 추출물은 프락토올리고당 및 갈락토올리고당보다 훨씬 건강기능성부분에서 우수한 프리바이오틱스로 사용될 수 있다는 것을 시사한다.The above results show that the pear extract of Example 2 is almost equivalent to fructooligosaccharide and galactooligosaccharide, which are products approved by the Ministry of Food and Drug Safety in terms of intestinal health function as prebiotics that promote the growth of probiotic strains, which are beneficial intestinal bacteria. The pear extract according to the present invention contains various anti-allergic ingredients, hangover detoxification ingredients, etc. including polyphenol antioxidants such as arbutin and chlorogenic acid, which are described below, unlike the above sugars (Fig. 5). It suggests that it can be used as a prebiotic that is superior to oligosaccharides and galactooligosaccharides in health functional areas.
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시 양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 즉, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다.As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, it is clear that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.
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