JP2592101B2 - Non-medical bactericidal composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to non-medical products for controlling harmful microorganisms generated in industrial or daily life products such as paints, wood and bamboo products, leather, glue and the like, or manufacturing processes thereof. The present invention relates to a disinfecting composition for use.

Conventionally, organic solvents and organotin agents have been widely used as non-medical germicidal compositions for controlling microorganisms generated in industrial products or in the process of producing them. However, the non-medical germicidal composition containing the organometallic compound as an active ingredient has been considered to have problems in residual toxicity and stability during drug use. Organic chlorinated compounds, quaternary ammonium salts, and the like have been used as alternative agents. However, recently, microbial disasters caused by bacteria, molds, and yeasts have frequently occurred, but these conventional drugs are not sufficiently effective, and there is a strong demand for the development of new drugs.

In view of such demands, the present inventors have conducted intensive studies to develop a non-medical bactericide having a low dose against many bacteria and fungi, and as a result, conventionally known as a bactericide alone, In addition, when used in combination with a specific compound that could not be sufficiently satisfied when used alone as a bactericide, a non-medical bactericidal composition having various excellent synergistic effects completely unpredictable from the efficacy of each compound alone Was found.

Thus, according to the present invention, there is provided a non-medical bactericide comprising (a) bitionol or phenticol or a salt thereof and (b) trihalogenated phenol or a salt thereof as an active ingredient.

The use of such a composition of the present invention provides an excellent synergistic bactericidal activity which is completely unpredictable due to the effects of using each of them alone.

In the present invention, (a) bithionol or fenticol is a compound represented by the following general formula (I).

[Wherein, M is selected from the group consisting of a hydrogen atom, an alkali metal and an alkaline earth metal, and X represents a halogen atom, and n represents an integer of 1 or 2. In the general formula (I), the case where n is 2 is bithionol, and the case where n is 1 is fuenchichlor.

The above-mentioned bithionol has been developed as a bactericide having an antibacterial action against bacteria and fungi, and is effective at inhibiting the growth of Staphylococcus aureus at a concentration of 1 / 100,000.
It is usually used in cosmetics, soaps, and shampoos, although it is usually used for the control of liver and intestinal dysfunctions and worms. Although it is prohibited to do so, it is still used as an antiseptic preservative for preventing the generation of algae, rubber products, and water-soluble polishing solutions.

Fuentichlor, on the other hand, was developed as an anti-infective antifungal agent for external use. In the present invention, bithionol and fuenchichlor may be used in combination.

On the other hand, in the present invention, the (b) trihalogenated phenol used in combination with the above-mentioned bitionol or phenticol is a compound represented by the following general formula (II).

[Wherein, M ′ is selected from the group consisting of a hydrogen atom, an alkali metal and an alkaline earth metal, and Y represents a halogen atom, but the three Ys may be the same or different. Specific examples of the above trihalogenated phenols include, for example, 2,4,6-trichlorophenol (hereinafter referred to as “2,4,6-TC
P "), 2,4,6-tribromophenol (hereinafter sometimes abbreviated as" 2,4,6-TBP "), 2,5
-Dichloro-4-bromophenol (hereinafter referred to as "2,5,4-DC
B "), 2,5-dichloro-6-bromophenol (hereinafter may be abbreviated as" 2,5,6-DCB "), and salts thereof. Can represent alkali metal salts or alkaline earth metal salts such as sodium, potassium or calcium salts.These trihalogenated phenols or salts thereof can be used for antifungal of water-based paint and slime control of papermaking. It is used as an antifungal agent for industrial use, an agricultural soil treatment agent, and a bactericidal and antifungal preservative for wood preservation, etc. However, trihalogenated phenol and its salts are remarkably high in volatility, so that the preventive effect is not maintained. There was a drawback in terms of utilization.

The non-medical germicidal composition of the present invention has a disadvantage as a single agent as described above and has a practical problem.
By making the composition in combination, not only the types of target microorganisms are expanded by simply adding the respective bactericidal effects,
Controls various molds and bacteria, which are microbes that caused microbial disasters and have been difficult to control, by using low doses, especially with control of 1/2 to 1/10 or less compared to the amount of single agent. An excellent synergistic effect was obtained.

Further, the composition of the present invention has an activity at a remarkably low concentration even against fungi of the genus Aspergillus and Trichoderma, which are microbial disaster-causing bacteria of industrial products, and can be controlled economically inexpensively and safely. It has an extremely excellent advantage.

In the composition of the present invention, (a) bithionol or fenticol or a salt thereof and (b) phenol trihalide or a salt thereof are in a ratio of 10: 1 to 1: 100 by weight, preferably 5: 1 to 1: 100. It is desirable to mix at a ratio of 20.

The composition of the present invention may contain the combination of the above two active ingredients, and may be in the form of being dispersed or dissolved in a carrier used for general sterilization and fungicide. Or a form mixed with a solid carrier. Also, if necessary, a spreading agent, a stabilizer, a dispersant,
Wetting agents, penetrants, suspending agents and the like can also be added. The dosage form of the composition may be any of a wettable powder, a powder, a tablet, an emulsion, a paste and the like.

The composition of the present invention may contain other fungicides, insecticides, and deterioration inhibitors.

The composition of the present invention can be applied directly as it is or diluted to an appropriate concentration depending on the object, purpose and form to be used.

Thus, the non-medical germicidal composition of the present invention can be used to generate harmful microorganisms by mixing or surface-treating paints, leather, wood and bamboo products, glues, woven and knitted products, paper products, wall materials, plastic products and the like. It is advantageously used to prevent the deterioration of the quality of these products because it prevents the growth of the products.

Hereinafter, the present invention will be described more specifically with reference to Examples, but the present invention is not limited thereto.

Minimum growth inhibitory concentration test by agar dilution method Experiment 1 Each drug is ethanol, and the active ingredient concentration is 4,8,10,15,20,25,30,40ppm, 2,4,6-TCP when the active ingredient concentration is bithionol.
In the case of, dilute to 5,10,20,30,40,50,60ppm and dispense 2cm to one 9cm Petri dish. Then, 10 ml of Sabouraud's medium having the following composition is heated and dissolved, and the mixture is thoroughly mixed with a drug diluent to form a solidified plate.

A spore solution was prepared on this by the method of JIS z-2911 and 10 ⁵
w / ml. Put it on agar with a microplater.
The spore solution was inoculated in 01 ml portions. After culturing at 28 ° C for one week, the presence or absence of growth of the bacteria was observed, and the minimum growth inhibitory concentration was determined. Some of the results are shown in Table 1 below.

Sabouraud medium composition Although the synergistic effect of the combined use of bitionol and 2,4,6-TCP is clear from Table 1 above in Experiment 1, the data of Experiment 1 are shown in FIGS.
(E). (A) to (E) in FIG. 1 correspond to those shown in the column of test bacteria shown in Table 1, respectively. In FIGS. 1A to 1E, the horizontal axis is 2,4,6-TCP
And the vertical axis represents the concentration of bitionol. In FIGS. 1A to 1E, the lowest growth inhibitory concentrations are shown below the dotted line, indicating that the combined use of bitionol and 2,4,6-TCP has a synergistic effect. Clearly shows.

Experiment 2 The experiment method was the same as in Experiment 1. However, instead of bitionol, use bitionol and fuenchichlor at 1:
Mix and use at a compounding ratio of 1, and use 1,2,5,10,
Experiments were performed at 20,50,100,200,400 ppm. Also 2,4,6-TCP
Was used at a similar concentration.

 Some of the results are shown in Table 2 below.

The results in Experiment 2 are shown in FIGS.
It was shown to. (A) to (F) in FIG. 2 correspond to (A) to (F) of the test bacteria in Table 2, respectively. All of them show that they have a synergistic effect.

Experiment 3 Each drug was ethanol and the active ingredient concentration was 1,2,5,10,20,5 in a mixture ratio of the concentration of the active ingredient bithionol + fuentichlor (1: 1).
0,100,200,400ppm, 2,4,6-TCP was also diluted to the same concentration, 9c
Dispense 2cm to one m Petri dish.

Then, 10 ml of the following standard medium is heated and dissolved, and the mixture is thoroughly mixed with a drug diluent to form a solidified plate.

Bacteria containing 10 ¹⁰ cells / ml in all cultures were inoculated with 0.01 ml of the bacteria on agar using a microplater.

After culturing at 37 ° C. for 48 hours, the presence or absence of growth of the bacteria was observed, and the minimum growth inhibitory concentration was determined.

Standard agar medium composition The results of the above experiment are shown in Table 3 below.

From the results, it can be understood that the effect of the present invention is also recognized against bacteria.

[Brief description of the drawings]

Fig. 1- (A)-Fig. 1- (E) and Fig. 2- (A)-
FIG. 2- (F) shows the relationship between the composition of the drug and the minimum inhibitory concentration obtained in Experiments 1 and 2 of the present invention.

Claims (3)

(57) [Claims] 1. A non-medical bactericidal composition comprising (a) bitionol or fenticol or a salt thereof, and (b) phenol trihalide or a salt thereof as active ingredients. 2. The non-medical germicidal composition according to claim 1, wherein said active ingredient is (a) bitionol or a salt thereof, and (b) trihalogenated phenol or a salt thereof. 3. The non-medical bactericidal composition according to claim 1, wherein said active ingredient is (a) fuenchichlor or a salt thereof, and (b) a phenol trihalide or a salt thereof.