JPS6058202B2 - Non-medical sterilizing composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to industrial products such as paints, wood and bamboo products, leather, pastes, etc., or industrial products necessary for our daily life, or non-medical products that control harmful microorganisms generated in the manufacturing process of these products. The present invention relates to a disinfectant composition for use in disinfectants.

Conventionally, non-medical sterilizing compositions for controlling microorganisms generated in industrial products or the process of manufacturing them include:
Mainly, organic solvents and organic tin agents were commonly used. However, concerns have arisen regarding the residual toxicity and stability of non-medical sterilizing compositions containing organometallic compounds as active ingredients during drug use. Organic chlorine compounds, quaternary ammonium salts, and the like have been used as alternative agents. However, recently, microbial disasters caused by bacteria, molds, and yeasts have been occurring frequently, and these drugs are not sufficiently effective against these, and there is a strong demand for the development of new drugs. In view of this demand, the present inventors have conducted intensive research to develop a disinfectant that is active against many bacteria and molds at low dosages, and have found that by using a mixture of known disinfectants, We have found that it is suitable for this purpose.

The present invention was made based on this knowledge,
It shows unexpected and excellent activity compared to when each of the conventionally known drugs is used alone.

Tetrachloroisophthalonitrile (hereinafter referred to as πN).

) was recently developed as an agricultural fungicide (Japanese Patent Publication No. 11358/1973), and has received high praise as a safe drug with almost no toxicity. However, TPN shows little therapeutic effect, ie, activity against already grown bacteria. In addition, as trihalogenated phenol compounds, 2,4,6-trichlorophenol (hereinafter referred to as 2,4,6-TCP), 2,4,5-trichlorophenol (hereinafter referred to as 2,4,6-TCP),
It is called 4.5-TCP. ), 2,4,6-tribromophenol (hereinafter referred to as 2,4,6-TBP).
), 2●5-dichloro-4-bromophenol (hereinafter 2
・It is called 5.4-DCB. ), 2.5-dichlororu6
- Bromophenol (hereinafter referred to as 2●5●6-DCB) and salts thereof are known, and these compounds have been used as agricultural soil treatment agents or industrial fungicides. However, these trihalogenated phenols and their salts have extremely high volatility, have poor preventive effects, and are unsatisfactory in terms of the durability of their medicinal efficacy. Furthermore, orthophenylphenol (hereinafter referred to as 0PP) or its salts is known as a highly safe preservative for citrus fruits that can be used as a food additive, and is generally active against various molds. However, its efficacy is slow and it is inferior to an industrial disinfectant, so it has not been put to practical use. However, the non-medical sterilizing composition of the present invention contains TPN or a trihalogenated phenolic compound or a salt thereof and OPP or a salt thereof as active ingredients.

That is, the present invention relates to TPN or the general formula (wherein M represents a hydrogen atom, an alkali metal atom, or an alkaline earth metal atom, and X represents a halogen atom).

) and 0PP or its alkali metal or alkaline earth metal salts as active ingredients, and are dispersed or dissolved in an appropriate carrier, or mixed with an appropriate solid carrier. However, if necessary, in addition to these, dispersants, suspension improvers, spreading agents, penetrants,
By adding wetting agents, stabilizers, etc., it is made into dosage forms such as wettable powders, powders, tablets, pastes, emulsions, and liquids. The non-medical sterilizing composition of the present invention may be applied directly or diluted to an appropriate concentration depending on the purpose of use. Furthermore, the non-medical sterilizing composition of the present invention may be used by blending other sterilizing agents, insecticides, deterioration inhibitors, etc. The trihalogenated phenols used in the present invention include the above-mentioned 2.4.
6-・TCPl2●4・5-TCPl2●4●6-TB
Pl2・4・5-TBPl2・5・4-DCBl2・5
●6-DCB and their sodium salts, potassium salts,
There are alkali metal salts or alkaline earth metal salts such as calcium salts. Active ingredient T in the non-medical sterilizing composition of the present invention
The mixing ratio of PN or trihalogenated phenol or its salts and 0PP or its salts varies depending on the purpose, but is usually TPN. (In the case of mixing 50PPs, the weight ratio is 1:0.1 to 10, preferably 1:0.5 to 3, and the weight ratio is 1:0.1 to 10, preferably 1:0.5 to 3.
When mixed with PP, 1:0.1-5, preferably 1
:0.2-2.

The non-medical sterilizing composition of the present invention inhibits the growth of harmful microorganisms by mixing or surface-treating paints, wood and bamboo products, leather, pastes, paper products, textiles, wall materials, vinyl chloride products, etc. and is used to prevent quality deterioration. Also,
It can also be used to prevent microbial contamination during the manufacturing process of these products. The non-medical sterilizing composition of the present invention has the above-mentioned drawbacks, and by mixing the drugs at a certain blending ratio, the sterilizing effects of each drug can be simply added up. Due to the synergistic effect of increasing the number of types of organisms, various molds and bacteria that cause microbial disasters and which were difficult to control in the past can be controlled with a low dosage. It is something that can be done. Furthermore, it should be noted that it is active at extremely low concentrations against molds of the genus Trichoderma, genus Aspergillus, and genus Cladosporium, as well as bacteria of the genus Pseudomonas and Aerobacter, which are bacteria that cause microbial hazards in industrial products. This means that it can be controlled economically and at low cost. Next, the present invention will be explained in more detail with reference to test examples and examples, but the scope of the present invention is not limited by these in any way.

The single agent TPN used in the test. ．． OPPl2●4●6-T
CPl2●5●4-DCBl2・5・6-DCB,. C
The PP-Ca salt was formulated into a 5% emulsion, and the others were formulated into a 10% solution. Experimental Example 1 (Minimum inhibitory concentration test using agar dilution method) Each drug was mixed with sterile water at an active ingredient concentration of 6.25, 12. \2\5
Diluted to 0, 75, 100 ppm, 9C77! Dispense 2 cm into two Petri dishes.

One Petri dish contained 18 ml of acidic Waxmann's medium and the other Petri dish contained 18 ml of modified Waxmann's medium.
Dissolve and add by heating, mix thoroughly with drug dilution solution, and form a solidified plate. For filamentous fungi, pre-grown bacterial flora agar was prepared using
Place the punched pieces with a TWL Gork borer on acidic Waxman medium, and inoculate the bacteria by taking a platinum loop on the slant and streaking it onto the modified Waxman medium. did. 28℃, 48
After I Terama culture, the presence or absence of bacterial growth was observed, and the minimum growth-inhibiting concentration was determined.

(1) Acidic Waxman medium composition Peptone 5V glucose
10y1KH2P041'Mg
SO4O. 59 Powdered agar 15y water
1e Just before use, adjust the pH to 4.5 with 25% lactic acid.

1 (2) Modified Waxman medium composition peptone
10y glucose
5yKH2P041yMgS040.5y2 Powder agar 12f water
1e Adjust the pH to 7.5 with 1110NNaOH before sterilization3.

Test bacteria 0 Aerobacter aerogenes IAM-1102 (A
erObacteraerOgenes) () Bacillus subtilis IAM-1069 (Bacillus sl.
lbtilis)C) Escherichia coli NIHJ (EscherichiacOll))) Pseudomonas ●Aeruginosa IAM-1054(P
aeudOmOnasaeruginOsa) Mi) Aspergillus●niger ATCC-6275 (Asper?
11usni? r)・゛) Penicillium●Citrinium ATCC-9849
trinum) ;) Rhizopus nigrinchus IAM
-6070 (RhizOpusnigricansη
Pullularia pullulans IAMF-24 (Pullul
ariapullulans) 1) Trichoderma T-
1ATCC-9645(TrichOderma)J)
Geotrichum andeiduum
lcandidum) Test Example 2 (Wood Mildew Prevention Test) A piece of wood (red pine) was immersed in a diluted solution of each chemical for 10 minutes, lightly drained, and air-dried for 2 hours.

Medium solidified on a plate (composition: glucose 40y)
A piece of wood treated with a chemical was placed on 1 peptone, 10 y, 1 agar, 25 y, and 1 e) of water, and the following sample bacterial spore mixed suspension was spray inoculated onto this wood piece. While culturing at 28°C±2°C, the growth of mold on the wood chips was examined over time.

The growth status of mold was divided into the following five stages depending on the degree of reproduction.

-No growth of mold was observed.

±: Slightly observed on the side and surface of the wood piece.

+: Mildew growth is less than one-third of the sides and surface of the wood piece +:
Grows to more than one-third.

+FF: Grows on the entire surface.

In addition, three groups of tests were conducted for each drug.

Test bacteria (4) Aspergillus niger ATCC-6275 (B
) Penicillium citrininum ATCC-9849 (C
) Rhizopus ● Nigrinchus S. Nl32(D) Cladosporium ● Herbalum IAM. F-517 (Clad
OspOriunlherbarum) (E) Chaetomium gloposum ATCC-6205 (ChaetOmi
umglObOsunl) Test Example 3 (Test of antiseptic efficacy of water-based paint) 27 parts of emulsion containing 50% acrylic resin (same parts by weight), 8 parts of titanium white, 23 parts of kaolin, 18 parts of heavy calcium carbonate, carboxymethyl cellulose (2 % solution) 98 g of an acrylic emulsion paint consisting of 1 part and 4 parts of water was placed in a can for tin-plated paint, and the chemicals were added to a predetermined concentration and mixed for 3 minutes using a tabletop stirrer.

Next, we investigated Pseudomonas aeruginosa IAM-1054, Aerobacter aerogenes IAM-1102, which are bacteria that cause microbial hazards in paint, and Aspergillus flavus ATCC-9643 (.Aspergil), which is a filamentous fungus.
lusflavus), Cladosporium herbalum IAM, . A mixed suspension of F5l7 was added to the paint in an amount of 1 mt for inoculation. In the case of bacteria, the inoculum is 1 x 1 Cf per 1 m1.
In the case of filamentous fungi, the solution was diluted to 1 x 103 cells and added to 1 ml. A broth liquid medium was used to dilute the inoculum to promote the growth of microorganisms. After inoculation, the can is sealed and kept in a constant temperature chamber at 28°C. The antiseptic efficacy is determined by the number of bacteria or filamentous fungi in 1 ml of paint 10 and 30 days after inoculation, and the antifungal efficacy is determined by the number of bacteria or fungi in 1 ml of paint after 10 and 30 days of inoculation. When the can was opened to measure the number of bacteria, the state of mold growth on the surface of the paint was checked and judged.

In addition, in order to determine the sustained efficacy of the drug, when measuring the number of bacteria 10 days later, a new inoculation of bacteria was performed in the same manner as the first time. The results are shown in Table 3.

"Example Middle" indicates parts by weight.

Example 1 Emulsion TPN 5 parts 0 PP 5 parts Solpol (surfactant manufactured by Toho Chemical Industry Co., Ltd.) 7 parts xylene
(4) Part Hysol#
100 (aromatic solvent manufactured by Nippon Petrochemical Co., Ltd.) and 33 parts or more are mixed to form an emulsion.

Example 2 hydrating agent TPN3 prostitute 0PP5 parts Neoperex P

Claims (1)

[Scope of Claims] 1. A non-medical sterilizing composition characterized by containing tetrachloroisophthalonitrile or trihalogenated phenol or its salts, and orthophenylphenol or its salts as active ingredients. 2. The non-medical sterilizing composition according to claim 1, wherein the active ingredients are tetrachloroisophthalonitrile and orthophenylphenol or salts thereof. 3. The non-medical sterilizing composition according to claim 1, wherein the active ingredients are trihalogenated phenol or its salts and orthophenylphenol or its salts.